This study aimed to investigate effects of shenkang injection on angiogenesis in mice with renal fibrosis. C57BL/6J mice after unilateral ureteral obstruction were administrated with different doses of shenkang injection for two weeks. Losartan-administrated mice and Sham-operation mice were as positive and negative controls, respectively. Renal function and vasculature changes were observed, and expressions of VEGF and VEGFR2 were detected. Shenkang injection reduced serum levels of urea nitrogen, creatinine and cystatin C. Shenkang injection alleviated the loss of microvessels, decreased the deposition of collagen fibers, and increased expressions of VEGF and VEGFR2 in the obstructed kidney. These findings indicate that shenkang injection alleviates renal dysfunction and fibrotic changes in mice with renal fibrosis by regulating renal angiogenesis. Video Clip of Methodology: Renal Vasculature by Angiography: 6 min 41 sec: Full Screen Alternate
{"title":"Effects of shenkang injection on renal angiogenesis in mice with unilateral ureteral obstruction","authors":"Yajing Pan, Lu Zhang, Tonghua Liu, Lili Wu","doi":"10.3329/bjp.v15i1.42052","DOIUrl":"https://doi.org/10.3329/bjp.v15i1.42052","url":null,"abstract":"This study aimed to investigate effects of shenkang injection on angiogenesis in mice with renal fibrosis. C57BL/6J mice after unilateral ureteral obstruction were administrated with different doses of shenkang injection for two weeks. Losartan-administrated mice and Sham-operation mice were as positive and negative controls, respectively. Renal function and vasculature changes were observed, and expressions of VEGF and VEGFR2 were detected. Shenkang injection reduced serum levels of urea nitrogen, creatinine and cystatin C. Shenkang injection alleviated the loss of microvessels, decreased the deposition of collagen fibers, and increased expressions of VEGF and VEGFR2 in the obstructed kidney. These findings indicate that shenkang injection alleviates renal dysfunction and fibrotic changes in mice with renal fibrosis by regulating renal angiogenesis. \u0000Video Clip of Methodology: \u0000Renal Vasculature by Angiography: 6 min 41 sec: Full Screen Alternate","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2020-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/bjp.v15i1.42052","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49444861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ali Semerci, Dilek Inceçayir, V. Mammadova, A. Hoş, K. Tunç
The antibacterial and antifungal activities of the bulb and flower of Allium staticiforme and Allium subhirsutum were investigated. In addition, DPPH radical scavenging activity and total phenolic contents were determined. The results show that methanolic extracts of A. staticiforme and A. subhirsutum had antifungal activities against Candida albicans, together with a less activity level against Escherichia coli, Staphylococcus epidermidis, S. aureus, Enterecoccus faecalis, Salmonella typhimurium and Pseudomanas aeruginosa. The total phenolic contents of A. staticiforme leaf and bulb were determined as 17 and 2.4 mg of GAE/100 g, respectively. The IC50 of methanolic extracts of A. staticiforme and A. subhirsutum were also determined. In conclusion, both A. staticiforme and A. subhirsutum have antifungal activities with weak antibacterial activities. These plants have DPPH radical scavenging activities.
{"title":"Antimicrobial activities of Allium staticiforme and Allium subhirsutum","authors":"Ali Semerci, Dilek Inceçayir, V. Mammadova, A. Hoş, K. Tunç","doi":"10.3329/bjp.v15i1.42373","DOIUrl":"https://doi.org/10.3329/bjp.v15i1.42373","url":null,"abstract":"The antibacterial and antifungal activities of the bulb and flower of Allium staticiforme and Allium subhirsutum were investigated. In addition, DPPH radical scavenging activity and total phenolic contents were determined. The results show that methanolic extracts of A. staticiforme and A. subhirsutum had antifungal activities against Candida albicans, together with a less activity level against Escherichia coli, Staphylococcus epidermidis, S. aureus, Enterecoccus faecalis, Salmonella typhimurium and Pseudomanas aeruginosa. The total phenolic contents of A. staticiforme leaf and bulb were determined as 17 and 2.4 mg of GAE/100 g, respectively. The IC50 of methanolic extracts of A. staticiforme and A. subhirsutum were also determined. In conclusion, both A. staticiforme and A. subhirsutum have antifungal activities with weak antibacterial activities. These plants have DPPH radical scavenging activities.","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2020-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/bjp.v15i1.42373","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46788358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Arisha Taj Mahaboob Batcha, A. Wadhwani, Gowri Subramaniam
The present study evaluates the antiviral activity of banana lectin (BanLec) against herpes simplex virus type 1 and 2 (HSV-1 and HSV-2). Lectin was isolated from the ripen pulp of bananas (Musa paradisiaca). The study showed that lectin exhibited hemagglutination activity towards human erythrocytes A, B, AB and O group. The molecular weight of BanLec using SDS gel-electrophoresis was found to be 14,000-30,000 Da. Cytotoxicity of BanLec on the Vero cell lines showed an inhibitory concentration of 172.7 µg/mL. BanLec was virucidal and showed no cytotoxicity at the concentration tested. The lectin showed a dose-dependent antiviral activities, inhibiting HSV-1 by 16.0 µg/mL with selectivity index 10.8 and HSV-2 inhibition by 67.7 µg/mL with selectivity index 2.6. These results corroborate that BanLec could be a rich source of potential antiviral compound for HSV-1 when compared to HSV-2.
{"title":"In vitro antiviral activity of BanLec against herpes simplex viruses type 1 and 2","authors":"Arisha Taj Mahaboob Batcha, A. Wadhwani, Gowri Subramaniam","doi":"10.3329/bjp.v15i1.42320","DOIUrl":"https://doi.org/10.3329/bjp.v15i1.42320","url":null,"abstract":"The present study evaluates the antiviral activity of banana lectin (BanLec) against herpes simplex virus type 1 and 2 (HSV-1 and HSV-2). Lectin was isolated from the ripen pulp of bananas (Musa paradisiaca). The study showed that lectin exhibited hemagglutination activity towards human erythrocytes A, B, AB and O group. The molecular weight of BanLec using SDS gel-electrophoresis was found to be 14,000-30,000 Da. Cytotoxicity of BanLec on the Vero cell lines showed an inhibitory concentration of 172.7 µg/mL. BanLec was virucidal and showed no cytotoxicity at the concentration tested. The lectin showed a dose-dependent antiviral activities, inhibiting HSV-1 by 16.0 µg/mL with selectivity index 10.8 and HSV-2 inhibition by 67.7 µg/mL with selectivity index 2.6. These results corroborate that BanLec could be a rich source of potential antiviral compound for HSV-1 when compared to HSV-2.","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2020-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/bjp.v15i1.42320","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49572920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The study was designed to see the effects of tetracycline and clotrimazole on the altered skin flora (Aspergillus and Enterobacter species) in 38 patients with palmar arsenical keratosis. The skin swab and scrapping samples were collected. Tetracycline, clotrimazole and their combination were given as ointment for 3 months. Clotrimazole and tetracycline were found to inhibit the growth of Aspergillus spp. and Enterobacter spp. in vitro. A pH-dependent inhibition of the growth of microorganisms in presence of these antimicrobials was observed, which was highest at pH 8. The percentage reduction of keratotic nodular size was 32.9, 66.1, 61.1 and 32.5 in the groups treated with placebo, tetracycline, clotrimazole and the combination of both, respectively. But none of the interventions was proved statistically significant. No remarkable adverse effect was reported. In conclusion, clotrimazole or tetracycline inhibits the growth of Aspergillus spp. or Enterobacter spp. in vitro. However, there was no statistically significant clinical improvement of the palmar arsenical keratosis.
{"title":"Effects of tetracycline and clotrimazole ointments in the treatment of palmar arsenical keratosis","authors":"A. Ferdous, M. Misbahuddin, Abu Naser Ibne Sattar","doi":"10.3329/bjp.v15i1.45249","DOIUrl":"https://doi.org/10.3329/bjp.v15i1.45249","url":null,"abstract":"The study was designed to see the effects of tetracycline and clotrimazole on the altered skin flora (Aspergillus and Enterobacter species) in 38 patients with palmar arsenical keratosis. The skin swab and scrapping samples were collected. Tetracycline, clotrimazole and their combination were given as ointment for 3 months. Clotrimazole and tetracycline were found to inhibit the growth of Aspergillus spp. and Enterobacter spp. in vitro. A pH-dependent inhibition of the growth of microorganisms in presence of these antimicrobials was observed, which was highest at pH 8. The percentage reduction of keratotic nodular size was 32.9, 66.1, 61.1 and 32.5 in the groups treated with placebo, tetracycline, clotrimazole and the combination of both, respectively. But none of the interventions was proved statistically significant. No remarkable adverse effect was reported. In conclusion, clotrimazole or tetracycline inhibits the growth of Aspergillus spp. or Enterobacter spp. in vitro. However, there was no statistically significant clinical improvement of the palmar arsenical keratosis.","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2020-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/bjp.v15i1.45249","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41695407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Sundar, L. Srikanth, P. S. Manognya, S. Yuvaraja, Sathiavelu Arunachalam
{"title":"In vitro antibacterial activity of Dracaena victoria leaf extract","authors":"R. Sundar, L. Srikanth, P. S. Manognya, S. Yuvaraja, Sathiavelu Arunachalam","doi":"10.3329/bjp.v14i4.43807","DOIUrl":"https://doi.org/10.3329/bjp.v14i4.43807","url":null,"abstract":"","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2019-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/bjp.v14i4.43807","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45887949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Improved mass screening of medicinal plants for antibacterial activity by Leaf Disc antibacterial assay","authors":"K. Keekan, S. Padmaraj","doi":"10.3329/bjp.v14i4.42657","DOIUrl":"https://doi.org/10.3329/bjp.v14i4.42657","url":null,"abstract":"","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2019-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/bjp.v14i4.42657","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45728685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kenza Bezza, Zineb El Gabbas, Jawad Laadraoui, M. Laaradia, Sara Oufquir, A. Chait
The present study evaluates the anticonvulsant activity of the roots of Anacyclus pyrethrum using pilocarpine-induced experimental model of epilepsy in rat, and to determine its possible anticonvulsant mechanism. Ethanol extract (200 and 400 mg/kg) or alkylamides (25 and 50 mg/kg) was administered orally 45 min before the injection of pilocarpine-induced (400 mg/kg) seizures. The possible anticonvulsant mechanism was investigated by testing the effect of atropine (2 mL/kg) and scopolamine (1 mg/kg). The scoring of seizure severity, seizures time latency, duration of total seizures and percentage of mortality protection were recorded. Ethanol extract and alkylamides prolonged the time of onset seizure and decreased the duration of seizures compared to control group (p<0.001). The seizure protection was 100%. The co-administered of ethanol extract of A. pyrethrum and alkylamides with atropine completely abolished the pilocarpine-induced seizures. Video Clip of Methodology: Anticonvulsant effect: 6 min 7 sec: Full Screen Alternate
{"title":"Ameliorative potential of Anacyclus pyrethrum extract in generalized seizures in rat: Possible cholinergic mediated mechanism","authors":"Kenza Bezza, Zineb El Gabbas, Jawad Laadraoui, M. Laaradia, Sara Oufquir, A. Chait","doi":"10.3329/bjp.v14i4.40537","DOIUrl":"https://doi.org/10.3329/bjp.v14i4.40537","url":null,"abstract":"The present study evaluates the anticonvulsant activity of the roots of Anacyclus pyrethrum using pilocarpine-induced experimental model of epilepsy in rat, and to determine its possible anticonvulsant mechanism. Ethanol extract (200 and 400 mg/kg) or alkylamides (25 and 50 mg/kg) was administered orally 45 min before the injection of pilocarpine-induced (400 mg/kg) seizures. The possible anticonvulsant mechanism was investigated by testing the effect of atropine (2 mL/kg) and scopolamine (1 mg/kg). The scoring of seizure severity, seizures time latency, duration of total seizures and percentage of mortality protection were recorded. Ethanol extract and alkylamides prolonged the time of onset seizure and decreased the duration of seizures compared to control group (p<0.001). The seizure protection was 100%. The co-administered of ethanol extract of A. pyrethrum and alkylamides with atropine completely abolished the pilocarpine-induced seizures. \u0000Video Clip of Methodology: \u0000Anticonvulsant effect: 6 min 7 sec: Full Screen Alternate","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2019-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/bjp.v14i4.40537","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45294739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pozdnyakov Dmitry, Nygaryan A. Syranusch, V. Andrey, A. Sosnovskaya, Cherechkova I. Elisaveta
The study was devoted to see the effect of ethylmethylhydroxypyridine succinate, acetylcysteine and choline alphoscerate on the mitochondrial function under condition of experimental cerebral ischemia in rat. An integrated approach was used with the assessment of changes in the respirometric and antiapoptotic functions of mitochondria. It was found that the application of the objects under study contributed to a decrease in the intensity of glycolysis and the restoration of aerobic glucose metabolism with an increase in ATP synthesis, as well as a decrease in the dissociation of reactions in mitochondrial complexes I, II, IV and V. In addition, the introduction of the studied drugs reduced the intensity of caspase-dependent and caspase-independent apoptosis reactions, which, together with the restoration of mitochondrial respiratory function, contributed to the decrease in the size of the necrosis zone of the brain. Video Clip of Methodology: Rat middle cerebral artery occlusion: 8 min 7 sec Full Screen Alternate
{"title":"Ethylmethylhydroxypyridine succinate, acetylcysteine and choline alphoscerate improve mitochondrial function under condition of cerebral ischemia in rat","authors":"Pozdnyakov Dmitry, Nygaryan A. Syranusch, V. Andrey, A. Sosnovskaya, Cherechkova I. Elisaveta","doi":"10.3329/bjp.v14i3.40977","DOIUrl":"https://doi.org/10.3329/bjp.v14i3.40977","url":null,"abstract":"The study was devoted to see the effect of ethylmethylhydroxypyridine succinate, acetylcysteine and choline alphoscerate on the mitochondrial function under condition of experimental cerebral ischemia in rat. An integrated approach was used with the assessment of changes in the respirometric and antiapoptotic functions of mitochondria. It was found that the application of the objects under study contributed to a decrease in the intensity of glycolysis and the restoration of aerobic glucose metabolism with an increase in ATP synthesis, as well as a decrease in the dissociation of reactions in mitochondrial complexes I, II, IV and V. In addition, the introduction of the studied drugs reduced the intensity of caspase-dependent and caspase-independent apoptosis reactions, which, together with the restoration of mitochondrial respiratory function, contributed to the decrease in the size of the necrosis zone of the brain. \u0000Video Clip of Methodology: \u0000Rat middle cerebral artery occlusion: 8 min 7 sec Full Screen Alternate","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2019-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/bjp.v14i3.40977","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44439074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Muhammad Abdur Rehman Shah, R. Khan, Mushtaq Ahmed
Phytochemical screening, cytotoxic activity, total phenolic content, antioxidant and anti-diabetic activities of the Sorghum halepense methanolic extract and its different fractions were evaluated. Methanolic extract and its various fractions revealed the presence of reducing sugars, tannins, steroids, glycosides, flavonoids in the methanolic extract where they were absent in n-hexane fraction except flavonoids. Gums and saponins were absent in all the samples. Methanolic extract indicated the highest cytotoxic (80.7 ± 1.3%) and anti-diabetic (62.5%) activities. The maximum total phenolic contents (28.7 ± 1.4 mg/mL) were found in the chloroform fraction. An aqueous fraction expressed the highest antioxidant activity 74.1 and 97.1% free radical scavenging properties in DPPH and ABTS assays respectively whereas, in the case of H2O2, methanolic extract indicated maximum (36.9%) activity. In conclusion, the extract of aerial parts of S. halepense is a source of compounds against cancer, diabetic and free radical associated disorders.
{"title":"Phytochemical analysis, cytotoxic, antioxidant and anti-diabetic activities of the aerial parts of Sorghum halepense","authors":"Muhammad Abdur Rehman Shah, R. Khan, Mushtaq Ahmed","doi":"10.3329/BJP.V14I3.40292","DOIUrl":"https://doi.org/10.3329/BJP.V14I3.40292","url":null,"abstract":"Phytochemical screening, cytotoxic activity, total phenolic content, antioxidant and anti-diabetic activities of the Sorghum halepense methanolic extract and its different fractions were evaluated. Methanolic extract and its various fractions revealed the presence of reducing sugars, tannins, steroids, glycosides, flavonoids in the methanolic extract where they were absent in n-hexane fraction except flavonoids. Gums and saponins were absent in all the samples. Methanolic extract indicated the highest cytotoxic (80.7 ± 1.3%) and anti-diabetic (62.5%) activities. The maximum total phenolic contents (28.7 ± 1.4 mg/mL) were found in the chloroform fraction. An aqueous fraction expressed the highest antioxidant activity 74.1 and 97.1% free radical scavenging properties in DPPH and ABTS assays respectively whereas, in the case of H2O2, methanolic extract indicated maximum (36.9%) activity. In conclusion, the extract of aerial parts of S. halepense is a source of compounds against cancer, diabetic and free radical associated disorders.","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2019-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/BJP.V14I3.40292","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43030277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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