Artificial Cells, Nanomedicine, and Biotechnology最新文献

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IF 4.5 3区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Artificial Cells, Nanomedicine, and Biotechnology

Pub Date : 2021-12-01 DOI: 10.1080/21691401.2021.1918483

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IF 5.8 3区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Artificial Cells, Nanomedicine, and Biotechnology

Pub Date : 2021-12-01 DOI: 10.1080/21691401.2021.1951991

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IF 5.8 3区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Artificial Cells, Nanomedicine, and Biotechnology

Pub Date : 2021-12-01 DOI: 10.1080/21691401.2021.1951955

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IF 5.8 3区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Artificial Cells, Nanomedicine, and Biotechnology

Pub Date : 2021-12-01 DOI: 10.1080/21691401.2021.1951984

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IF 5.8 3区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Artificial Cells, Nanomedicine, and Biotechnology

Pub Date : 2021-12-01 DOI: 10.1080/21691401.2021.1951969

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Synthesis of silver nanoparticles using Ziziphus nummularia leaf extract and evaluation of their antimicrobial, antioxidant, cytotoxic and genotoxic potential (4-in-1 system). 利用菰叶提取物合成银纳米粒子，并评估其抗菌、抗氧化、细胞毒性和基因毒性潜力（四合一系统）。

IF 4.5 3区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Artificial Cells, Nanomedicine, and Biotechnology

Pub Date : 2021-12-01 DOI: 10.1080/21691401.2021.1903478

Hemali Padalia, Sumitra Chanda

This study reports the synthesis of silver nanoparticles (AgNPs) from silver nitrate by leaf extract of a medicinal plant Ziziphus nummularia. The leaf extract acts as a reducing and stabilizing agent for the formation of nanoparticles. The green synthesized AgNPs were characterized by ultraviolet-visible (UV-vis) spectroscopy, Fourier transform infrared (FITR) spectroscopy, Thermogravimetric analysis (TGA), X-ray diffraction (XRD), transmission electron microscopy (TEM) analysis and evaluated their antimicrobial, antioxidant, cytotoxic and genotoxic potential. The UV-Vis spectroscopy showed a characteristic absorption peak at 430 nm due to surface plasma resonance. TEM analysis showed that synthesized AgNPs were spherical and oval with an average size of 25.96 nm. AgNPs showed effective antimicrobial activity (lowest MIC-0.625 µg/mL against Escherichia coli), synergistic antimicrobial activity (lowest ΣFIC 0.09 with chlormaphenicol against Corynebacterium rubrum) and antibiofilm activity. AgNPs showed strong DPPH activity with IC₅₀ - 520 µg/mL and ABTS activity IC₅₀ - 55 µg/mL and reducing capacity assessment. In vitro cytotoxic effect was evaluated by MTT assay against HeLa cells, breast cells and fibroblast cells. Genotoxic effect was evaluated by comet assay. AgNPs displayed dose-dependent cytotoxic and genotoxic effect. Our findings indicated that synthesized AgNPs could be considered as multifunctional and have great potential for use in biomedical applications.HighlightsSilver nanoparticles were synthesized using leaf extract of Ziziphus nummulariaCharacterization was done by various spectral techniquesAntimicrobial efficacy was demonstrated against an array of bacteriaAgNPs exhibited significant cytotoxic effect against HeLa cell lineAgNPs showed cytotoxicity and genotoxicity in a dose-dependent manner.

本研究报告了利用一种药用植物酸枣仁（Ziziphus nummularia）的叶提取物从硝酸银中合成银纳米粒子（AgNPs）的过程。叶提取物是形成纳米粒子的还原剂和稳定剂。通过紫外可见光谱（UV-vis）、傅立叶变换红外光谱（FITR）、热重分析（TGA）、X 射线衍射（XRD）、透射电子显微镜（TEM）分析，对绿色合成的 AgNPs 进行了表征，并评估了其抗菌、抗氧化、细胞毒性和遗传毒性潜力。紫外可见光谱显示，由于表面等离子体共振，在 430 纳米处出现了特征吸收峰。TEM 分析表明，合成的 AgNPs 呈球形和椭圆形，平均尺寸为 25.96 nm。AgNPs 显示出有效的抗菌活性（对大肠杆菌的最低 MIC 值为 0.625 µg/mL）、协同抗菌活性（与氯苯酚一起对红醋栗棒状杆菌的最低 ΣFIC 值为 0.09）和抗生物膜活性。AgNPs 显示出很强的 DPPH 活性（IC50 - 520 µg/mL）和 ABTS 活性（IC50 - 55 µg/mL），并进行了还原能力评估。体外细胞毒性效应是通过 MTT 法对 HeLa 细胞、乳腺细胞和成纤维细胞进行评估的。基因毒性效应通过彗星试验进行评估。AgNPs 显示出了剂量依赖性的细胞毒性和基因毒性效应。研究结果表明，合成的 AgNPs 具有多功能性，在生物医学应用中具有巨大的潜力。亮点利用菰叶提取物合成了银纳米粒子，并通过各种光谱技术对其进行了表征。

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Statement of Retraction. 撤回声明。

IF 5.8 3区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Artificial Cells, Nanomedicine, and Biotechnology

Pub Date : 2021-12-01 DOI: 10.1080/21691401.2021.1899438

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Expression of Concern. 表达关心。

IF 5.8 3区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Artificial Cells, Nanomedicine, and Biotechnology

Pub Date : 2021-12-01 DOI: 10.1080/21691401.2021.1899409

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Expression of Concern. 表达关心。

IF 5.8 3区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Artificial Cells, Nanomedicine, and Biotechnology

Pub Date : 2021-12-01 DOI: 10.1080/21691401.2021.1899384

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Targeting regulation of the tumour microenvironment induces apoptosis of breast cancer cells by an affinity hemoperfusion adsorbent. 通过亲和血液灌流吸附剂靶向调节肿瘤微环境诱导乳腺癌细胞凋亡

IF 4.5 3区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Artificial Cells, Nanomedicine, and Biotechnology

Pub Date : 2021-12-01 DOI: 10.1080/21691401.2021.1902337

Lichun Wang, Jian Chen, Yamin Chai, Wenyan Han, Jie Shen, Nan Li, Jinyan Lu, Yunzheng Du, Zhuang Liu, Yameng Yu, Jingzhe Dong, Lailiang Ou

The cytokine network of tumour microenvironment (TME) plays an important role in cancer growth and progression. The current work aims to provide a new strategy for cancer therapy based on the targeted regulation of cytokines in the TME. Here, heparin-coupled polyvinyl alcohol (PVA-H) microspheres have been developed as an adsorbent for selectively remove tumour-induced immunosuppressive cytokines, such as vascular endothelial growth factor (VEGF) and transforming growth factor-beta (TGF-β), but not tumour necrosis factor-alpha (TNF-α) which has an immune-stimulating effect and can inhibit tumour growth. The proliferation and apoptosis of breast cancer cells after perfusion were tested by cell viability assays, flow cytometry analysis and mRNA microarray assays. Results showed that the PVA-H microspheres efficiently absorbed the majority of VEGF (74.39%) and TGF-β (86.39%), but much less TNF-α (4.16%). The regulation of the cytokines had remarkable anti-proliferative and pro-apoptotic effects on breast cancer cells, which was further confirmed from the change of mRNA expression levels. Thus, targeting regulatory pathways within the TME by an affinity adsorbent that selectively depletes immunosuppressive cytokines is potentially a new and promising strategy for cancer therapy.

肿瘤微环境（TME）的细胞因子网络在癌症的生长和进展中发挥着重要作用。目前的工作旨在提供一种基于靶向调节肿瘤微环境细胞因子的癌症治疗新策略。肝素偶联聚乙烯醇（PVA-H）微球被开发成一种吸附剂，可选择性地去除肿瘤诱导的免疫抑制细胞因子，如血管内皮生长因子（VEGF）和转化生长因子-β（TGF-β），但不包括具有免疫刺激作用并能抑制肿瘤生长的肿瘤坏死因子-α（TNF-α）。通过细胞活力测定、流式细胞仪分析和 mRNA 微阵列检测了灌注后乳腺癌细胞的增殖和凋亡情况。结果表明，PVA-H 微球能有效吸收大部分 VEGF（74.39%）和 TGF-β（86.39%），但 TNF-α（4.16%）的吸收率较低。细胞因子的调节对乳腺癌细胞具有显著的抗增殖和促凋亡作用，这一点从 mRNA 表达水平的变化中得到了进一步证实。因此，通过亲和吸附剂选择性地去除免疫抑制细胞因子来靶向TME内的调控通路，可能是一种有前途的癌症治疗新策略。

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