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Honeyberry-derived carbon quantum dots ameliorate LPS-induced neuroinflammation and oxidative stress through Nrf2/HO-1 signalling in HMC3 cells 蜂蜜衍生的碳量子点通过Nrf2/HO-1信号传导改善lps诱导的HMC3细胞的神经炎症和氧化应激
IF 5.8 3区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2023-02-22 DOI: 10.1080/21691401.2023.2179062
Sanjay, Anshul Sharma, Hae-Jeung Lee

Abstract

Carbon quantum dots (CQDs) were synthesized from blue honeysuckle (Lonicera caerulea) berry fruit extracts using a well-known, cost-effective, and environmental friendly hydrothermal process. The material was characterized using UV-vis spectroscopy, photoluminescence (PL), XPS, and TEM studies. The as-synthesized carbon dots exhibit excellent PL properties, with a quantum yield of ∼35.92%. CQDs vary in size from ∼2 nm to 9 nm. This study established the neuroprotective effects of CQDs against lipopolysaccharide (LPS)-induced human microglial cell model. LPS was found to induce cytotoxicity, reactive oxygen species, and pro-inflammatory cytokines interleukin (IL)-1β, IL-6, and tumour necrosis factor-α) and downregulated enzymatic antioxidants such as nuclear factor-erythroid factor 2-related factor 2 (Nrf2), superoxide dismutase, catalase, haem oxygenase (HO)-1, HO-2, and glutathione peroxidase, while CQDs treatment reversed LPS induced cytotoxicity, induced anti-inflammatory cytokines (IL-4, IL-10, and transforming growth factor β) and induce enzymatic antioxidants both at transcriptional and translational levels. The study suggested the potential role of CQDs prepared from Lonicera caerulea, as anti-inflammatory and antioxidative agents in neuroinflammatory and neurodegenerative diseases. In addition, CQDs could be exploited in various biomedical applications such as biosensing, drug delivery and tissue engineering.

摘要以蓝金银花(Lonicera caerulea)浆果提取物为原料,采用水热法合成碳量子点(CQDs)。利用紫外可见光谱、光致发光(PL)、XPS和TEM对材料进行了表征。合成的碳点具有优异的PL性能,量子产率为~ 35.92%。cqd的尺寸从~ 2nm到9nm不等。本研究建立了CQDs对脂多糖(LPS)诱导的人小胶质细胞模型的神经保护作用。研究发现,LPS可诱导细胞毒性、活性氧、促炎细胞因子白介素(IL)-1β、IL-6和肿瘤坏死因子-α,并下调核因子-红因子-2相关因子2 (Nrf2)、超氧化物歧化酶、过氧化氢酶、血红素加氧酶(HO)-1、HO-2和谷胱甘肽过氧化物酶等酶促抗氧化剂,而CQDs可逆转LPS诱导的细胞毒性、诱导抗炎细胞因子(IL-4、IL-10、IL-10)。和转化生长因子β),并在转录和翻译水平诱导酶抗氧化剂。提示金银花CQDs在神经炎症和神经退行性疾病中具有抗炎和抗氧化作用。此外,CQDs还可用于生物传感、药物传递和组织工程等多种生物医学应用。
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引用次数: 1
Statement of Retraction 撤回声明
IF 5.8 3区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-12-31 DOI: 10.1080/21691401.2022.2060567
N. Asadi, N. Annabi, E. Mostafavi, Maryam Anzabi, Rovshan Khalilov, Siamak Saghfi, Masoud Mehrizadeh, A. Akbarzadeh
Nahideh Asadi, Nasim Annabi, Ebrahim Mostafavi, Maryam Anzabi, Rovshan Khalilov, Siamak Saghfi, Masoud Mehrizadeh & Abolfazl Akbarzadeh (2018) Synthesis, characterization and in vitro evaluation of magnetic nanoparticles modified with PCL–PEG–PCL for controlled delivery of 5FU. Artificial Cells, Nanomedicine, and Biotechnology, 46(sup1), 938–945, DOI: https:// doi.org/10.1080/21691401.2018.1439839
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引用次数: 0
Statement of Retraction 撤回声明
IF 5.8 3区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-12-31 DOI: 10.1080/21691401.2022.2060561
Eommolbanin Ebrahimi, Amir Ahmad Khandaghi, F. Valipour, Soraia Babaie, Fatemeh Asghari, Soheila Motaali, E. Abbasi, A. Akbarzadeh, S. Davaran
The reused images have been described as originating from a new study with no reference to the previous studies. We contacted the corresponding authors, and they acknowledged the similarities in the data published. As this error directly impacts the reported results and conclusions, the Editor and Publisher have agreed to retract the article to ensure correction of the scholarly record. The corresponding author has been informed.
重复使用的图像被描述为来自一项新的研究,没有参考以前的研究。我们联系了通讯作者,他们承认发表的数据有相似之处。由于这个错误直接影响了报告的结果和结论,编辑和出版商同意撤回文章,以确保学术记录的纠正。已通知通讯作者。
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引用次数: 0
Statement of Retraction 撤回声明
IF 5.8 3区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-12-31 DOI: 10.1080/21691401.2022.2060559
Eommolbanin Ebrahimi, A. Akbarzadeh, E. Abbasi, Amir Ahmad Khandaghi, Farhad Abasalizadeh, S. Davaran
Figure 5 appears to have been duplicated with Figure 6 from Ebrahimi et al., 2014 (https://doi.org/10.3109/21691401. 2014.968822). Figure 6 appears to have been duplicated with Figure 7 from Ebrahimi et al., 2014 (https://doi.org/10.3109/21691401. 2014.968822) Figure 6 appears to have been duplicated with Figure 11 from Akbarzadeh et al., 2012 (https://doi.org/10.2147/ IJN.S24326) Figure 7 appears to have been duplicated with Figure 8 from Ebrahimi et al., 2014 (https://doi.org/10.3109/21691401. 2014.968822).
图5似乎与Ebrahimi等人2014年的图6重复(https://doi.org/10.3109/21691401)。2014.968822)。图6似乎与Ebrahimi等人2014年的图7重复(https://doi.org/10.3109/21691401)。2014.968822)图6似乎与Akbarzadeh等人,2012年的图11 (https://doi.org/10.2147/ ijs . s24326)图7似乎与Ebrahimi等人,2014年的图8 (https://doi.org/10.3109/21691401)重复。2014.968822)。
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引用次数: 0
Statement of Retraction 撤回声明
IF 5.8 3区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-12-31 DOI: 10.1080/21691401.2022.2054518
Linlin Wang, Xiaonan Zhao, Ye Wang
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引用次数: 0
Statement of Retraction 撤回声明
IF 5.8 3区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-12-31 DOI: 10.1080/21691401.2022.2054515
Sen Wei, Jinghao Liu, Xin Li, Xing Liu
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引用次数: 0
Oxidative stress and histopathological changes in several organs of mice injected with biogenic silver nanoparticles. 生物源性纳米银注射小鼠多个器官的氧化应激和组织病理学改变。
IF 5.8 3区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-12-01 DOI: 10.1080/21691401.2022.2149931
Shushanik Kazaryan, Lilit Farsiyan, Juleta Tumoyan, Gayane Kirakosyan, Naira Ayvazyan, Hrachik Gasparyan, Sona Buloyan, Lilit Arshakyan, Ara Kirakosyan, Ashkhen Hovhannisyan

The widespread use of silver nanoparticles (AgNPs) requires a study of their safety. The aim of the present study was to assess the levels of oxidative stress markers and histopathological changes in the experimental model of sarcoma S-180 of outbred mice caused by biogenic AgNPs. AgNPs were synthesized using 50% ethanol extract of Ocimum araratum leaves that was standardized for rosmarinic acid content. The effects of AgNPs were tested on chemiluminescence (ChL), malonic dialdehyde (MDA) content and activity of superoxide dismutase (SOD) in healthy and experimental model of sarcoma S-180 mice. It was shown that, under the influence of AgNPs, the intensity of ChL decreased, in contrast with control groups (with the exception of the hepatocytes of animals with transplanted sarcoma). The presence of AgNPs leads to the decrease of MDA in the tissues of healthy mice and to a slight increase of MDA content in the tumour and kidney tissues. AgNPs neutralize the activity of SOD in kidney tissue samples in animals with transplanted sarcoma, and in tumour tissue, they reduce SOD activity by three times. The results of the histological analysis indicate that AgNPs not only cause the destruction of tumour tissue but also lead to structural changes in hepatocytes and nephrons, which can affect the function of these organs. AgNPs are potential agents for antitumor therapy. Future studies are needed using biocompatible non-toxic NPs that meet the requirement for these drugs.

银纳米粒子(AgNPs)的广泛使用需要对其安全性进行研究。本研究的目的是评估生物源性AgNPs引起的远交种小鼠S-180肉瘤实验模型的氧化应激标志物水平和组织病理学变化。以迷迭香酸含量为标准的香木香叶50%乙醇提取物为原料合成AgNPs。研究AgNPs对S-180肉瘤健康小鼠和实验小鼠化学发光(ChL)、丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性的影响。结果表明,在AgNPs的影响下,与对照组相比,ChL的强度降低(移植肉瘤动物的肝细胞除外)。AgNPs的存在导致健康小鼠组织中MDA含量降低,肿瘤组织和肾脏组织中MDA含量略有增加。AgNPs中和移植肉瘤动物肾组织样品中SOD的活性,在肿瘤组织中,它们使SOD活性降低三倍。组织学分析结果表明,AgNPs不仅会破坏肿瘤组织,还会导致肝细胞和肾单位的结构改变,从而影响这些器官的功能。AgNPs是抗肿瘤治疗的潜在药物。未来的研究需要使用符合这些药物要求的生物相容性无毒NPs。
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引用次数: 1
Potential applications of PEGylated green gold nanoparticles in cyclophosphamide-induced cystitis 聚乙二醇化绿金纳米颗粒在环磷酰胺性膀胱炎中的潜在应用
IF 5.8 3区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-12-01 DOI: 10.1080/21691401.2022.2078340
Bushra Shal, Safa Amanat, A. Khan, You Jeong Lee, H. Ali, F. Din, Youmie Park, Salman Khan
Abstract We investigated the effect of green tea extract PEGylated gold nanoparticles (P-AuNPs) making use of its targeted and sustained drug delivery against cyclophosphamide (CYP)-induced cystitis. AuNPs were synthesized by reduction reaction of gold salts with green tea extract following the concept of green synthesis. Mostly spherical-shaped P-AuNPs were synthesized with an average size of 14.3 ± 3.3 nm. Pre-treatment with P-AuNPs (1, 10 mg/kg, i.p.) before CYP (150 mg/kg, i.p.) challenge suggested its uroprotective properties. P-AuNPs significantly reversed all pain-like behaviours and toxicities produced by CYP resulting in a decreased aspartate aminotransferase, alanine aminotransferase, C-reactive protein, and creatinine level. P-AuNPs increased anti-oxidant system by increasing the level of reduced glutathione, glutathione-S-transferase, catalase and superoxide dismutase, and reduced nitric oxide production in bladder tissue. Additionally, it attenuated hypokalaemia and hyponatremia, along with a decrease in Evans blue content in bladder tissue and peritoneal cavity. CYP-induced bladder tissue damage observed by macroscopic and histological findings were remarkably attenuated by P-AuNPs, along with reduced fibrosis of collagen fibre in bladder smooth muscles shown by Masson’s trichrome staining. Additionally, alterations in hematological parameters and clinical scoring were also prevented by P-AuNPs suggesting its uroprotective effect.
摘要:我们研究了绿茶提取物聚乙二醇化金纳米颗粒(P-AuNPs)利用其靶向和持续给药治疗环磷酰胺(CYP)诱导的膀胱炎的作用。采用绿色合成的思路,将金盐与绿茶提取物还原反应合成AuNPs。合成的P-AuNPs多为球形,平均尺寸为14.3±3.3 nm。在注射CYP (150 mg/kg, i.p.)之前,先用P-AuNPs (1,10 mg/kg, i.p.)进行预处理,表明其具有泌尿保护作用。P-AuNPs显著逆转了CYP产生的所有疼痛样行为和毒性,导致天冬氨酸转氨酶、丙氨酸转氨酶、c反应蛋白和肌酐水平下降。P-AuNPs通过增加膀胱组织还原性谷胱甘肽、谷胱甘肽- s转移酶、过氧化氢酶和超氧化物歧化酶的水平和减少一氧化氮的产生来增强抗氧化系统。此外,它还能减轻低钾血症和低钠血症,同时减少膀胱组织和腹膜腔中的埃文斯蓝含量。P-AuNPs可显著减轻cyp诱导的膀胱组织损伤,马松三色染色显示膀胱平滑肌胶原纤维纤维化减轻。此外,P-AuNPs还可以预防血液学参数和临床评分的改变,这表明其具有尿保护作用。
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引用次数: 2
Antibacterial, antioxidant, and haemolytic potential of silver nanoparticles biosynthesized using roots extract of Cannabis sativa plant. 用大麻根提取物生物合成纳米银的抗菌、抗氧化和溶血潜能。
IF 5.8 3区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-12-01 DOI: 10.1080/21691401.2022.2149543
Suman Suman, Lacy Loveleen, Meena Bhandari, Asad Syed, Ali H Bahkali, Romila Manchanda, Surendra Nimesh

In this study, Cannabis sativa roots extract has been employed for the biosynthesis of silver nanoparticles (AgNPs). The appearance of reddish-brown colour followed by absorption peak of AgNPs at 408 nm through UV-vis spectrophotometry suggested biosynthesis of AgNPs. The size of the particles ranged from 90-113 nm, confirmed using DLS and TEM along with zeta potential of -25.3 mV. The FTIR provided information regarding the phytochemical capping. The study was further elaborated for determining AgNPs antibacterial, antioxidant, and cellular toxicity using MIC, DPPH, MTT, and haemolytic assays, respectively. The AgNPs were significantly effective against Staphylococcus aureus (Gram-positive), as compared to that of Pseudomonas aeruginosa, Klebsiella pneumoniae, and Escherichia coli (Gram-negative). AgNPs also exhibited remarkable antioxidant potential wherein 58.01 ± 0.09% free radical scavenging was observed at a concentration of 100 µg/ml. AgNPs revealed lower cytotoxicity where cell viability was observed to be 52.38 ± 0.6% at a very high concentration of 500 µg/ml in HEK 293 cells. Further, very low toxicity was seen in RBCs i.e. 6.47 ± 0.04% at a high concentration of 200 µg/ml. Thus, the current study beholds anticipation that Cannabis sativa ethanolic root extract-mediated AgNPs may play a vital role in therapeutic.

在这项研究中,大麻根提取物已被用于银纳米粒子(AgNPs)的生物合成。紫外-可见分光光度法测定AgNPs在408 nm处出现吸收峰,呈红褐色,表明AgNPs是生物合成的。经DLS和TEM测定,颗粒尺寸在90 ~ 113 nm之间,zeta电位为-25.3 mV。FTIR提供了有关植物化学封顶的信息。该研究进一步阐述了AgNPs的抗菌、抗氧化和细胞毒性,分别使用MIC、DPPH、MTT和溶血试验。与铜绿假单胞菌、肺炎克雷伯菌和大肠杆菌(革兰氏阴性)相比,AgNPs对金黄色葡萄球菌(革兰氏阳性)明显有效。AgNPs还表现出显著的抗氧化能力,当浓度为100 μ g/ml时,其自由基清除率为58.01±0.09%。AgNPs表现出较低的细胞毒性,在500µg/ml的极高浓度下,HEK 293细胞的细胞活力为52.38±0.6%。此外,在高浓度200µg/ml时,对红细胞的毒性非常低,为6.47±0.04%。因此,目前的研究可以预见,大麻乙醇根提取物介导的AgNPs可能在治疗中发挥重要作用。
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引用次数: 2
Treatment-related adverse events of PD-1/PD-L1 inhibitors combined with CTLA-4 inhibitors in clinical trials: a meta-analysis. 临床试验中PD-1/PD-L1抑制剂联合CTLA-4抑制剂治疗相关不良事件:一项荟萃分析
IF 5.8 3区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-12-01 DOI: 10.1080/21691401.2022.2131354
Ze Mi, Yunshu Zhang, Zhichao Feng, Jiahao Liu, Jianmin Wu, Hongpei Tan, Xiaoqian Ma, Zhenguo Liu, Pengfei Rong

Aim: PD-1/PD-L1 inhibitors in combination with CTLA-4 inhibitors are being tested in a number of ongoing clinical trials. As a result, it is critical to fully comprehend the toxicity characteristics of adverse events in combination therapy. This study aims to extensively compare the incidences and ORs of treatment-related adverse events between two combination strategies.

Methods: The eligible articles were searched from PubMed, EMBASE and Cochrane databases for studies published between 1 January 2010 and 1 May 2021, investigating PD-1/PD-L1 inhibitors plus CTLA-4 inhibitor-based combined clinical therapies. The mean incidences and pooled ORs of all-grade and grade 3 or higher adverse events were calculated by random-effects model using Stata 12.1. Heterogeneity between studies was assessed with I2 statistics and Chi square-based Q statistic. The overall risk of bias was assessed by Review Manager 5.3.

Results: A total of 26 eligible studies of 3607 patients were selected; 2852 patients developed at least one all-grade adverse event. PD-L1 inhibitors plus CTLA-4 inhibitors regimen (incidence 0.67, 95% CI: 0.57-0.77) had marked advantage over PD-1 inhibitors plus CTLA-4 inhibitors regimen (incidence 0.89, 95% CI: 0.86-0.93).

Conclusion: PD-L1 inhibitors plus CTLA-4 inhibitors shows better safety in treatment-related adverse events than PD-1 inhibitors plus CTLA-4 inhibitors.

目的:PD-1/PD-L1抑制剂联合CTLA-4抑制剂正在一些正在进行的临床试验中进行测试。因此,充分了解联合治疗中不良事件的毒性特征是至关重要的。本研究旨在广泛比较两种联合治疗策略的治疗相关不良事件的发生率和or。方法:从PubMed、EMBASE和Cochrane数据库中检索2010年1月1日至2021年5月1日发表的研究,研究PD-1/PD-L1抑制剂加CTLA-4抑制剂联合临床治疗。采用Stata 12.1随机效应模型计算所有级别和3级及以上不良事件的平均发生率和合并or。采用I2统计量和基于卡方的Q统计量评估研究间的异质性。总体偏倚风险由Review Manager 5.3进行评估。结果:共纳入26项符合条件的研究,共纳入3607例患者;2852例患者出现至少一次全级别不良事件。PD-L1抑制剂+ CTLA-4抑制剂方案(发生率0.67,95% CI: 0.57-0.77)明显优于PD-1抑制剂+ CTLA-4抑制剂方案(发生率0.89,95% CI: 0.86-0.93)。结论:PD-L1抑制剂联合CTLA-4抑制剂治疗相关不良事件的安全性优于PD-1抑制剂联合CTLA-4抑制剂。
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引用次数: 2
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Artificial Cells, Nanomedicine, and Biotechnology
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