Pub Date : 2023-09-04DOI: 10.1080/21691401.2023.2255635
Yanchun Gao, Ruihong Wang, Lin Liu, Shitao Feng, Xiaozhi Xi, Wengong Yu, Yuchao Gu, Ye Wang
Helicobacter pylori (H. pylori) is recognized as a pathogen associated with several gastrointestinal diseases. The current treatments exhibit numerous drawbacks, including antibiotic resistance. H. pylori can adhere to and colonize the gastric mucosa through H. pylori adhesin A (HpaA), and antibodies against HpaA may be an effective therapeutic approach. The variable domain of immunoglobulin new antigen receptor (VNAR) is a novel type of single-domain antibody with a small size, good stability, and easy manufacturability. This study isolated VNARs against HpaA from an immune shark VNAR phage display library. The VNARs can bind both recombinant and native HpaA proteins. The VNARs, 2A2 and 3D6, showed high binding affinities to HpaA with different epitopes. Furthermore, homodimeric bivalent VNARs, biNb-2A2 and biNb-3D6, were constructed to enhance the binding affinity. The biNb-2A2 and biNb-3D6 had excellent stability at gastrointestinal pH conditions. Finally, a sandwich ELISA assay was developed to quantify the HpaA protein using BiNb-2A2 as the capture antibody and BiNb-3D6 as the detection antibody. This study provides a potential foundation for novel alternative approaches to treatment or diagnostics applications of H. pylori infection.
幽门螺杆菌(Helicobacter pylori, H. pylori)被认为是一种与多种胃肠道疾病相关的病原体。目前的治疗方法有许多缺点,包括抗生素耐药性。幽门螺杆菌可通过幽门螺杆菌粘连素A (HpaA)粘附胃黏膜并定植,抗HpaA抗体可能是治疗幽门螺杆菌的有效途径。免疫球蛋白新抗原受体可变结构域(variable domain of immunoglobulin new antigen receptor, VNAR)是一种体积小、稳定性好、易于制造的新型单域抗体。本研究从免疫鲨鱼VNAR噬菌体展示文库中分离出抗HpaA的VNAR。vnas可以结合重组蛋白和天然HpaA蛋白。vnas 2A2和3D6对不同表位的HpaA具有较高的结合亲和力。此外,构建了二价同源二聚体vnas biNb-2A2和biNb-3D6,以增强其结合亲和力。biNb-2A2和biNb-3D6在胃肠道pH条件下具有良好的稳定性。最后,以BiNb-2A2为捕获抗体,BiNb-3D6为检测抗体,建立夹心ELISA法定量HpaA蛋白。本研究为幽门螺杆菌感染的治疗或诊断提供了新的替代方法的潜在基础。
{"title":"Identification and characterization of shark VNARs targeting the <i>Helicobacter pylori</i> adhesin HpaA.","authors":"Yanchun Gao, Ruihong Wang, Lin Liu, Shitao Feng, Xiaozhi Xi, Wengong Yu, Yuchao Gu, Ye Wang","doi":"10.1080/21691401.2023.2255635","DOIUrl":"https://doi.org/10.1080/21691401.2023.2255635","url":null,"abstract":"<p><p><i>Helicobacter pylori</i> (<i>H. pylori</i>) is recognized as a pathogen associated with several gastrointestinal diseases. The current treatments exhibit numerous drawbacks, including antibiotic resistance. <i>H. pylori</i> can adhere to and colonize the gastric mucosa through <i>H. pylori</i> adhesin A (HpaA), and antibodies against HpaA may be an effective therapeutic approach. The variable domain of immunoglobulin new antigen receptor (VNAR) is a novel type of single-domain antibody with a small size, good stability, and easy manufacturability. This study isolated VNARs against HpaA from an immune shark VNAR phage display library. The VNARs can bind both recombinant and native HpaA proteins. The VNARs, 2A2 and 3D6, showed high binding affinities to HpaA with different epitopes. Furthermore, homodimeric bivalent VNARs, biNb-2A2 and biNb-3D6, were constructed to enhance the binding affinity. The biNb-2A2 and biNb-3D6 had excellent stability at gastrointestinal pH conditions. Finally, a sandwich ELISA assay was developed to quantify the HpaA protein using BiNb-2A2 as the capture antibody and BiNb-3D6 as the detection antibody. This study provides a potential foundation for novel alternative approaches to treatment or diagnostics applications of <i>H. pylori</i> infection.</p>","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":null,"pages":null},"PeriodicalIF":5.8,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10211558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-22DOI: 10.1080/21691401.2023.2179062
Sanjay, Anshul Sharma, Hae-Jeung Lee
Abstract
Carbon quantum dots (CQDs) were synthesized from blue honeysuckle (Lonicera caerulea) berry fruit extracts using a well-known, cost-effective, and environmental friendly hydrothermal process. The material was characterized using UV-vis spectroscopy, photoluminescence (PL), XPS, and TEM studies. The as-synthesized carbon dots exhibit excellent PL properties, with a quantum yield of ∼35.92%. CQDs vary in size from ∼2 nm to 9 nm. This study established the neuroprotective effects of CQDs against lipopolysaccharide (LPS)-induced human microglial cell model. LPS was found to induce cytotoxicity, reactive oxygen species, and pro-inflammatory cytokines interleukin (IL)-1β, IL-6, and tumour necrosis factor-α) and downregulated enzymatic antioxidants such as nuclear factor-erythroid factor 2-related factor 2 (Nrf2), superoxide dismutase, catalase, haem oxygenase (HO)-1, HO-2, and glutathione peroxidase, while CQDs treatment reversed LPS induced cytotoxicity, induced anti-inflammatory cytokines (IL-4, IL-10, and transforming growth factor β) and induce enzymatic antioxidants both at transcriptional and translational levels. The study suggested the potential role of CQDs prepared from Lonicera caerulea, as anti-inflammatory and antioxidative agents in neuroinflammatory and neurodegenerative diseases. In addition, CQDs could be exploited in various biomedical applications such as biosensing, drug delivery and tissue engineering.
{"title":"Honeyberry-derived carbon quantum dots ameliorate LPS-induced neuroinflammation and oxidative stress through Nrf2/HO-1 signalling in HMC3 cells","authors":"Sanjay, Anshul Sharma, Hae-Jeung Lee","doi":"10.1080/21691401.2023.2179062","DOIUrl":"https://doi.org/10.1080/21691401.2023.2179062","url":null,"abstract":"<p><b>Abstract</b></p><p>Carbon quantum dots (CQDs) were synthesized from blue honeysuckle (<i>Lonicera caerulea</i>) berry fruit extracts using a well-known, cost-effective, and environmental friendly hydrothermal process. The material was characterized using UV-vis spectroscopy, photoluminescence (PL), XPS, and TEM studies. The as-synthesized carbon dots exhibit excellent PL properties, with a quantum yield of ∼35.92%. CQDs vary in size from ∼2 nm to 9 nm. This study established the neuroprotective effects of CQDs against lipopolysaccharide (LPS)-induced human microglial cell model. LPS was found to induce cytotoxicity, reactive oxygen species, and pro-inflammatory cytokines interleukin (IL)-1β, IL-6, and tumour necrosis factor-α) and downregulated enzymatic antioxidants such as nuclear factor-erythroid factor 2-related factor 2 (Nrf2), superoxide dismutase, catalase, haem oxygenase (HO)-1, HO-2, and glutathione peroxidase, while CQDs treatment reversed LPS induced cytotoxicity, induced anti-inflammatory cytokines (IL-4, IL-10, and transforming growth factor β) and induce enzymatic antioxidants both at transcriptional and translational levels. The study suggested the potential role of CQDs prepared from <i>Lonicera caerulea</i>, as anti-inflammatory and antioxidative agents in neuroinflammatory and neurodegenerative diseases. In addition, CQDs could be exploited in various biomedical applications such as biosensing, drug delivery and tissue engineering.</p>","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":null,"pages":null},"PeriodicalIF":5.8,"publicationDate":"2023-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138526999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-31DOI: 10.1080/21691401.2022.2060561
Eommolbanin Ebrahimi, Amir Ahmad Khandaghi, F. Valipour, Soraia Babaie, Fatemeh Asghari, Soheila Motaali, E. Abbasi, A. Akbarzadeh, S. Davaran
The reused images have been described as originating from a new study with no reference to the previous studies. We contacted the corresponding authors, and they acknowledged the similarities in the data published. As this error directly impacts the reported results and conclusions, the Editor and Publisher have agreed to retract the article to ensure correction of the scholarly record. The corresponding author has been informed.
{"title":"Statement of Retraction","authors":"Eommolbanin Ebrahimi, Amir Ahmad Khandaghi, F. Valipour, Soraia Babaie, Fatemeh Asghari, Soheila Motaali, E. Abbasi, A. Akbarzadeh, S. Davaran","doi":"10.1080/21691401.2022.2060561","DOIUrl":"https://doi.org/10.1080/21691401.2022.2060561","url":null,"abstract":"The reused images have been described as originating from a new study with no reference to the previous studies. We contacted the corresponding authors, and they acknowledged the similarities in the data published. As this error directly impacts the reported results and conclusions, the Editor and Publisher have agreed to retract the article to ensure correction of the scholarly record. The corresponding author has been informed.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":null,"pages":null},"PeriodicalIF":5.8,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72905606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-31DOI: 10.1080/21691401.2022.2060559
Eommolbanin Ebrahimi, A. Akbarzadeh, E. Abbasi, Amir Ahmad Khandaghi, Farhad Abasalizadeh, S. Davaran
Figure 5 appears to have been duplicated with Figure 6 from Ebrahimi et al., 2014 (https://doi.org/10.3109/21691401. 2014.968822). Figure 6 appears to have been duplicated with Figure 7 from Ebrahimi et al., 2014 (https://doi.org/10.3109/21691401. 2014.968822) Figure 6 appears to have been duplicated with Figure 11 from Akbarzadeh et al., 2012 (https://doi.org/10.2147/ IJN.S24326) Figure 7 appears to have been duplicated with Figure 8 from Ebrahimi et al., 2014 (https://doi.org/10.3109/21691401. 2014.968822).
{"title":"Statement of Retraction","authors":"Eommolbanin Ebrahimi, A. Akbarzadeh, E. Abbasi, Amir Ahmad Khandaghi, Farhad Abasalizadeh, S. Davaran","doi":"10.1080/21691401.2022.2060559","DOIUrl":"https://doi.org/10.1080/21691401.2022.2060559","url":null,"abstract":"Figure 5 appears to have been duplicated with Figure 6 from Ebrahimi et al., 2014 (https://doi.org/10.3109/21691401. 2014.968822). Figure 6 appears to have been duplicated with Figure 7 from Ebrahimi et al., 2014 (https://doi.org/10.3109/21691401. 2014.968822) Figure 6 appears to have been duplicated with Figure 11 from Akbarzadeh et al., 2012 (https://doi.org/10.2147/ IJN.S24326) Figure 7 appears to have been duplicated with Figure 8 from Ebrahimi et al., 2014 (https://doi.org/10.3109/21691401. 2014.968822).","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":null,"pages":null},"PeriodicalIF":5.8,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78788246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-31DOI: 10.1080/21691401.2022.2054518
Linlin Wang, Xiaonan Zhao, Ye Wang
{"title":"Statement of Retraction","authors":"Linlin Wang, Xiaonan Zhao, Ye Wang","doi":"10.1080/21691401.2022.2054518","DOIUrl":"https://doi.org/10.1080/21691401.2022.2054518","url":null,"abstract":"","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":null,"pages":null},"PeriodicalIF":5.8,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88104941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-01DOI: 10.1080/21691401.2022.2027428
Chie Okuda, Hiromi Sakai
Carbon monoxide (CO) is a toxic gas that causes neuropathy. However, CO is endogenously produced in small amounts showing various beneficial effects. We hypothesized that CO-bound haemoglobin-vesicle (HbV) administration would reduce cerebral ischaemia-reperfusion injury without causing neuropathy. Three experiments were conducted. First, rats were exposed to CO inhalation to create a CO-poisoning group, and they were sacrificed on 0, 7, 14, and 21 days after CO exposure. Histopathologically, hippocampal damage was prominent at 14 days. Second, the rats were administered with CO-HbV equivalent to 50 or 25% of circulating blood volume (CO-HbV50 or CO-HbV25 group). Rats were sacrificed 14 days after administration. Third, rats put into haemorrhagic shock by 50% of circulating blood withdrawal were resuscitated using saline, autologous blood, and CO-HbV. They were sacrificed 14 days after resuscitation. Hippocampal damage assessment clarified that almost no necrotic cells were observed in the CO-HbV50 group. Necrotic cells in the CO-HbV25 group were comparable to those found for the control group. In rats resuscitated from haemorrhagic shock, the hippocampal damage in the group using CO-HbV was the mildest. Administration of CO-HbV did not lead to marked hippocampal damage. Furthermore, CO-HbV was effective at preventing cerebral ischaemia-reperfusion injury after haemorrhagic shock.
{"title":"Effect of carbon monoxide administration using haemoglobin-vesicles on the hippocampal tissue.","authors":"Chie Okuda, Hiromi Sakai","doi":"10.1080/21691401.2022.2027428","DOIUrl":"https://doi.org/10.1080/21691401.2022.2027428","url":null,"abstract":"<p><p>Carbon monoxide (CO) is a toxic gas that causes neuropathy. However, CO is endogenously produced in small amounts showing various beneficial effects. We hypothesized that CO-bound haemoglobin-vesicle (HbV) administration would reduce cerebral ischaemia-reperfusion injury without causing neuropathy. Three experiments were conducted. First, rats were exposed to CO inhalation to create a CO-poisoning group, and they were sacrificed on 0, 7, 14, and 21 days after CO exposure. Histopathologically, hippocampal damage was prominent at 14 days. Second, the rats were administered with CO-HbV equivalent to 50 or 25% of circulating blood volume (CO-HbV50 or CO-HbV25 group). Rats were sacrificed 14 days after administration. Third, rats put into haemorrhagic shock by 50% of circulating blood withdrawal were resuscitated using saline, autologous blood, and CO-HbV. They were sacrificed 14 days after resuscitation. Hippocampal damage assessment clarified that almost no necrotic cells were observed in the CO-HbV50 group. Necrotic cells in the CO-HbV25 group were comparable to those found for the control group. In rats resuscitated from haemorrhagic shock, the hippocampal damage in the group using CO-HbV was the mildest. Administration of CO-HbV did not lead to marked hippocampal damage. Furthermore, CO-HbV was effective at preventing cerebral ischaemia-reperfusion injury after haemorrhagic shock.</p>","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":null,"pages":null},"PeriodicalIF":5.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39863122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-01DOI: 10.1080/21691401.2022.2103237
{"title":"Expression of Concern.","authors":"","doi":"10.1080/21691401.2022.2103237","DOIUrl":"https://doi.org/10.1080/21691401.2022.2103237","url":null,"abstract":"","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":null,"pages":null},"PeriodicalIF":5.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40565713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-01DOI: 10.1080/21691401.2022.2103238
{"title":"Expression of Concern.","authors":"","doi":"10.1080/21691401.2022.2103238","DOIUrl":"https://doi.org/10.1080/21691401.2022.2103238","url":null,"abstract":"","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":null,"pages":null},"PeriodicalIF":5.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40641059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}