Pub Date : 2022-06-30DOI: 10.22487/bioceb.v16i1.15833
E. Mursyida, Fifi Candita, M. Faisal, Deinike Wanita Marwan
Diarrhea is a potential extraordinary event that can cause death. The most common microbes that cause diarrhea in developing countries are Rotavirus and Escherichia coli. One of the treatment of diarrhea is by giving antibiotics. However, the use of antibiotics is known to disrupt the balance of normal gastrointestinal flora, thereby changing the composition of the microbiota. Probiotics can be given to balance the normal flora during or after treatment with antibiotics. Lactic acid bacteria (LAB) is one of the probiotics that can inhibit the growth of pathogenic microbes and is commonly found in fermented foods. This study aimed to isolate LAB from tempoyak made from kampar durian, Riau Province, Indonesia and test its antimicrobial activity against Escherichia coli. The study was initiated by isolating LAB from tempoyak using the multilevel dilution method, followed by characterizing LAB by colony and cell morphology, and testing its antimicrobial activity against Escherichia coli using the agar well diffusion method. The antimicrobial activity test results were analyzed using one-way ANOVA and Bonferroni follow-up test. The results of LAB characterization showed that four LAB isolates had different colony morphology, including spherical Gram-positive bacteria and catalase-negative bacteria. The results of the antimicrobial activity test showed that LAB1 isolates had the highest ability to inhibit Escherichia coli with an average inhibition zone diameter of 15.21 mm and the lowest inhibition zone was found in LAB 4 isolates, which was 10.80 mm. The results of the one way ANOVA test showed a significant difference between the four LAB isolates in inhibiting Escherichia coli with P value <0.05. In the Bonferroni test, there were significant differences between LAB 1 and LAB 4 isolates. It can be concluded that LAB isolated from tempoyak has the potential to be a source of probiotics.
{"title":"ISOLATION OF LACTIC ACID BACTERIA FROM TEMPOYAK AND ITS ANTIMICROBIAL ACTIVITY ON THE Escherichia coli","authors":"E. Mursyida, Fifi Candita, M. Faisal, Deinike Wanita Marwan","doi":"10.22487/bioceb.v16i1.15833","DOIUrl":"https://doi.org/10.22487/bioceb.v16i1.15833","url":null,"abstract":"Diarrhea is a potential extraordinary event that can cause death. The most common microbes that cause diarrhea in developing countries are Rotavirus and Escherichia coli. One of the treatment of diarrhea is by giving antibiotics. However, the use of antibiotics is known to disrupt the balance of normal gastrointestinal flora, thereby changing the composition of the microbiota. Probiotics can be given to balance the normal flora during or after treatment with antibiotics. Lactic acid bacteria (LAB) is one of the probiotics that can inhibit the growth of pathogenic microbes and is commonly found in fermented foods. This study aimed to isolate LAB from tempoyak made from kampar durian, Riau Province, Indonesia and test its antimicrobial activity against Escherichia coli. The study was initiated by isolating LAB from tempoyak using the multilevel dilution method, followed by characterizing LAB by colony and cell morphology, and testing its antimicrobial activity against Escherichia coli using the agar well diffusion method. The antimicrobial activity test results were analyzed using one-way ANOVA and Bonferroni follow-up test. The results of LAB characterization showed that four LAB isolates had different colony morphology, including spherical Gram-positive bacteria and catalase-negative bacteria. The results of the antimicrobial activity test showed that LAB1 isolates had the highest ability to inhibit Escherichia coli with an average inhibition zone diameter of 15.21 mm and the lowest inhibition zone was found in LAB 4 isolates, which was 10.80 mm. The results of the one way ANOVA test showed a significant difference between the four LAB isolates in inhibiting Escherichia coli with P value <0.05. In the Bonferroni test, there were significant differences between LAB 1 and LAB 4 isolates. It can be concluded that LAB isolated from tempoyak has the potential to be a source of probiotics.","PeriodicalId":8780,"journal":{"name":"Biocelebes","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80596889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-30DOI: 10.22487/bioceb.v16i1.15832
Ayu Natalia BR Tarigan, Eddy Nurtjahya, T Alief Aththorick
Tumbuhan berkhasiat obat sudah sejak lama dimanfaatkan oleh masyarakat Etnis Karo, Kecamatan Sinembah Tanjung Muda Hulu sebagai alternatif pencegahan dan penyembuhan penyakit. Penggunaan obat tradisional sebagai alternatif pengobatan lebih banyak dibanding pengobatan secara medis. Penelitian ini bertujuan untuk mengetahui pengetahuan, kepercayaan akan khasiat obat, jenis tumbuhan, bagian tumbuhan yang digunakan, jenis ramuan, cara pengolahan, dan nilai index of cultural significance (ICS) bahan baku yang digunakan. Penelitian ini menggunakan metode wawancara semistruktur untuk responden umum, dan wawancara bebas untuk informan kunci. Dari hasil penelitian, responden dengan kategori usia dewasa hingga tua memiliki pengetahuan dan kepercayaan yang lebih tinggi dalam penggunaan obat tradisional. Sebelas jenis ramuan obat tradisional diperoleh baik yang disajikan secara tunggal dan campuran yang berasal dari 36 jenis tumbuhan dari 21 famili. Bagian tumbuhan paling sering digunakan adalah daun, dan nilai ICS tertinggi (178) adalah jahe. Tumbuhan obat diramu dengan direbus, digiling, atau dikeringkan.
{"title":"OBAT TRADISIONAL PENINGKAT IMUNITAS TUBUH ETNIS KARO, KECAMATAN SINEMBAH TANJUNG MUDA HULU","authors":"Ayu Natalia BR Tarigan, Eddy Nurtjahya, T Alief Aththorick","doi":"10.22487/bioceb.v16i1.15832","DOIUrl":"https://doi.org/10.22487/bioceb.v16i1.15832","url":null,"abstract":"Tumbuhan berkhasiat obat sudah sejak lama dimanfaatkan oleh masyarakat Etnis Karo, Kecamatan Sinembah Tanjung Muda Hulu sebagai alternatif pencegahan dan penyembuhan penyakit. Penggunaan obat tradisional sebagai alternatif pengobatan lebih banyak dibanding pengobatan secara medis. Penelitian ini bertujuan untuk mengetahui pengetahuan, kepercayaan akan khasiat obat, jenis tumbuhan, bagian tumbuhan yang digunakan, jenis ramuan, cara pengolahan, dan nilai index of cultural significance (ICS) bahan baku yang digunakan. Penelitian ini menggunakan metode wawancara semistruktur untuk responden umum, dan wawancara bebas untuk informan kunci. Dari hasil penelitian, responden dengan kategori usia dewasa hingga tua memiliki pengetahuan dan kepercayaan yang lebih tinggi dalam penggunaan obat tradisional. Sebelas jenis ramuan obat tradisional diperoleh baik yang disajikan secara tunggal dan campuran yang berasal dari 36 jenis tumbuhan dari 21 famili. Bagian tumbuhan paling sering digunakan adalah daun, dan nilai ICS tertinggi (178) adalah jahe. Tumbuhan obat diramu dengan direbus, digiling, atau dikeringkan.","PeriodicalId":8780,"journal":{"name":"Biocelebes","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85603271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-30DOI: 10.22487/bioceb.v16i1.15664
Emilia Juliyanti Bria
Desa Dualaus di Kecamatan Kakuluk Mesak Kabupaten Belu dikenal sebagai Desa industri kerajinan anyaman yang menghasilkan produk beragam dan bermanfaat bagi kehidupan masyarakat. Informasi tentang penggunaan tumbuhan sebagai bahan anyaman masih sangat terbatas. Penelitian ini bertujuan untuk mengetahui jenis dan bagian tumbuhan yang digunakan, proses pembuatan kerajinan anyaman dan produk kerajinan anyaman serta kegunaannya yang dihasilkan di Desa Dualaus Kecamatan Kakuluk Mesak Kabupaten Belu. Metode yang digunakan dalam penelitian ini adalah survei eksploratif dan wawancara. Hasil penelitian menunjukan bahwa jenis tumbuhan yang digunakan sebagai bahan baku kerajinan anyaman adalah pandan hutan berduri (Pandanus tectorius Sol.), lontar (Borassus flabellifer Linn.) dan gewang (Corypha utan Lamk.). Bagian tumbuhan yang digunakan adalah daun dan lidi. Produk anyaman yang dihasilkan sebanyak 15 jenis yang dimanfaatkan sebagai peralatan rumah tangga, upacara adat, aksesoris dan fashion serta perlengkapan sekolah.
{"title":"ETNOBOTANI KERAJINAN ANYAMAN DI DESA DAULAUS KABUPATEN BELU NUSA TENGGARA TIMUR INDONESIA","authors":"Emilia Juliyanti Bria","doi":"10.22487/bioceb.v16i1.15664","DOIUrl":"https://doi.org/10.22487/bioceb.v16i1.15664","url":null,"abstract":"Desa Dualaus di Kecamatan Kakuluk Mesak Kabupaten Belu dikenal sebagai Desa industri kerajinan anyaman yang menghasilkan produk beragam dan bermanfaat bagi kehidupan masyarakat. Informasi tentang penggunaan tumbuhan sebagai bahan anyaman masih sangat terbatas. Penelitian ini bertujuan untuk mengetahui jenis dan bagian tumbuhan yang digunakan, proses pembuatan kerajinan anyaman dan produk kerajinan anyaman serta kegunaannya yang dihasilkan di Desa Dualaus Kecamatan Kakuluk Mesak Kabupaten Belu. Metode yang digunakan dalam penelitian ini adalah survei eksploratif dan wawancara. Hasil penelitian menunjukan bahwa jenis tumbuhan yang digunakan sebagai bahan baku kerajinan anyaman adalah pandan hutan berduri (Pandanus tectorius Sol.), lontar (Borassus flabellifer Linn.) dan gewang (Corypha utan Lamk.). Bagian tumbuhan yang digunakan adalah daun dan lidi. Produk anyaman yang dihasilkan sebanyak 15 jenis yang dimanfaatkan sebagai peralatan rumah tangga, upacara adat, aksesoris dan fashion serta perlengkapan sekolah.","PeriodicalId":8780,"journal":{"name":"Biocelebes","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74366460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Corn straw and coffee bean waste as a medium and source of nutrients needed for the growth of mycelium white oyster mushroom (P. ostreatus). This research has been carried out at the Microbiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Tadulako University. The purpose of this study was to determine the growth of mycelium and the characteristics of white oyster mushroom (P. ostreatus) in the formulation of corn straw and coffee bean waste media. This study was designed in a completely randomized design (CRD), consisting of seven treatments and three replications, namely P1 (100% corn straw powder), P2 (95% corn straw powder + 5% coffee bean waste), P3 (90% corn straw powder + 10% coffee bean waste), P4 (85% corn straw powder + 15% coffee bean waste), P5 (80% corn straw powder + 20% coffee bean waste), P6 (75% corn straw powder + 25% coffee bean waste), P7 (70% sawdust + 20% rice bran + 10% corn flour as a positive control). The results showed that corn straw and coffee waste which were used for growth media of white oyster mushroom mycelium (P.ostreatus) could be used as basic media because mycelium could grow well in all treatments. The growth rate of white oyster mushroom mycelium in P3 treatment enjoyed very fast growth and P6 treatment showed very slow growth. The highest number of colonies was in treatment P3 6.40x1011CFU/grand the lowest number of colonies was in treatment P6 2.66x1011CFU/g. Treatment P3 takes a shorter time to fill the culture bottles, which is 30 days, while P6 takes a longer time to fill the culture bottles, which is 48 days.
{"title":"PERTUMBUHAN MISELIUM JAMUR TIRAM PUTIH (Pleurotus ostreatus) PADA MEDIA FORMULA JERAMI JAGUNG DAN LIMBAH BIJI KOPI","authors":"Umrah Umrah, Eny Yuniati, Amiruddin Kasim, Kirana Kirana","doi":"10.22487/bioceb.v16i1.15950","DOIUrl":"https://doi.org/10.22487/bioceb.v16i1.15950","url":null,"abstract":"Corn straw and coffee bean waste as a medium and source of nutrients needed for the growth of mycelium white oyster mushroom (P. ostreatus). This research has been carried out at the Microbiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Tadulako University. The purpose of this study was to determine the growth of mycelium and the characteristics of white oyster mushroom (P. ostreatus) in the formulation of corn straw and coffee bean waste media. This study was designed in a completely randomized design (CRD), consisting of seven treatments and three replications, namely P1 (100% corn straw powder), P2 (95% corn straw powder + 5% coffee bean waste), P3 (90% corn straw powder + 10% coffee bean waste), P4 (85% corn straw powder + 15% coffee bean waste), P5 (80% corn straw powder + 20% coffee bean waste), P6 (75% corn straw powder + 25% coffee bean waste), P7 (70% sawdust + 20% rice bran + 10% corn flour as a positive control). The results showed that corn straw and coffee waste which were used for growth media of white oyster mushroom mycelium (P.ostreatus) could be used as basic media because mycelium could grow well in all treatments. The growth rate of white oyster mushroom mycelium in P3 treatment enjoyed very fast growth and P6 treatment showed very slow growth. The highest number of colonies was in treatment P3 6.40x1011CFU/grand the lowest number of colonies was in treatment P6 2.66x1011CFU/g. Treatment P3 takes a shorter time to fill the culture bottles, which is 30 days, while P6 takes a longer time to fill the culture bottles, which is 48 days.","PeriodicalId":8780,"journal":{"name":"Biocelebes","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75875004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-13DOI: 10.22487/bioceb.v15i2.15574
Yosea Adinata
Rotan mempunyai manfaat sebagai bahan baku kerajinan seperti keranjang, caping, tikar, nyiru, sapu, dan alat pengikat. Hutan Danau Selogan memiliki lingkungan yang cocok sebagai tempat tumbuhnya tanaman rotan. Penelitian tentang “inventarisasi rotan di hutan lindung danau selogan Kapuas Hulu”, Desa Ujung Said, Kecamatan Jongkong, Kabupaten Kapuas Hulu, Kalimantan Barat, telah dilaksanakan, penelitian ini bertujuan untuk mengetahui jenis-jenis rotan yang ada di hutan lindung Danau Selogan. Metode penelitian ini adalah deskriptif. Sampel diambil menggunakan teknik purposive sampling dengan metode jelajah. Berdasarkan hasil penelitian ditemukan 6 jenis tumbuhan rotan, yaitu Korthalsia rigida Blume, Calamus pasphalantus Beccari, Daemonorops sabut Beccari, Korthalsia junghuhnii Blume, Calamus caesius Blume, dan Korthalsia echinometra Beccari.
{"title":"INVENTARISASI ROTAN DI HUTAN LINDUNG DANAU SELOGAN KAPUAS HULU KALIMANTAN BARAT","authors":"Yosea Adinata","doi":"10.22487/bioceb.v15i2.15574","DOIUrl":"https://doi.org/10.22487/bioceb.v15i2.15574","url":null,"abstract":"Rotan mempunyai manfaat sebagai bahan baku kerajinan seperti keranjang, caping, tikar, nyiru, sapu, dan alat pengikat. Hutan Danau Selogan memiliki lingkungan yang cocok sebagai tempat tumbuhnya tanaman rotan. Penelitian tentang “inventarisasi rotan di hutan lindung danau selogan Kapuas Hulu”, Desa Ujung Said, Kecamatan Jongkong, Kabupaten Kapuas Hulu, Kalimantan Barat, telah dilaksanakan, penelitian ini bertujuan untuk mengetahui jenis-jenis rotan yang ada di hutan lindung Danau Selogan. Metode penelitian ini adalah deskriptif. Sampel diambil menggunakan teknik purposive sampling dengan metode jelajah. Berdasarkan hasil penelitian ditemukan 6 jenis tumbuhan rotan, yaitu Korthalsia rigida Blume, Calamus pasphalantus Beccari, Daemonorops sabut Beccari, Korthalsia junghuhnii Blume, Calamus caesius Blume, dan Korthalsia echinometra Beccari.","PeriodicalId":8780,"journal":{"name":"Biocelebes","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77186313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-13DOI: 10.22487/bioceb.v15i2.15607
Umrah Umrah
Penelitian ini perlu di lakukan karena pada limbah tandan kosong kelapa sawit dan dedak padi merupakan sumber nutrisi yang bernilai ekonomi tinggi yang dapat dimanfaatkan sebagai media pertumbuhan jamur tiram putih (Pleurotus ostreatus). Tujuan penelitian ini adalah (1) mengetahui pertumbuhan miselium jamur tiram putih (P. ostreatus) pada substrat limbah tandan kosong kelapa sawit dan dedak padi; (2) mengetahui karakteristik pertumbuhan jamur tiram putih (P. ostreatus) pada perlakuan jenis substrat yang berbeda. Penelitian ini telah di lakukan dalam rancangan acak lengkap (RAL) yang terdiri tujuh perlakuan dan tiga kali pengulangan. Susunan perlakuan merupakan perbandingan antara tandan kosong kelapa sawit : dedak padi, yakni P1 (50% : 50%), P2 (45% : 55%), P3 (40% : 60%), P4 (35% : 65%). P5 (30% : 70%), P6 (25% : 75%), P7 (serbuk geragaji 70%, dedak padi 20%, tepung jagung 10%). Adapun hasil penelitian menunjukkan laju pertumbuhan miselium tercepat pada perlakuan P1 yaitu 89 mm dan terendah pada perlakuan P5 yaitu 80 mm. Jumlah koloni tertinggi pada perlakuan P5 yaitu 1,4x1012 CFU/g dan terendah pada perlakuan P4 yaitu 0,4x1012 CFU/g. Waktu inkubasi paling cepat pada perlakuan P4 yaitu 28 hari dan terendah pada perlakuan P7 yaitu 33 hari.
{"title":"POTENSI EKONOMI LIMBAH TANDAN KOSONG KELAPA SAWIT DAN DEDAK PADI SEBAGAI MEDIA TUMBUH MISELIUM JAMUR TIRAM PUTIH (Pleurotus ostreatus)","authors":"Umrah Umrah","doi":"10.22487/bioceb.v15i2.15607","DOIUrl":"https://doi.org/10.22487/bioceb.v15i2.15607","url":null,"abstract":"Penelitian ini perlu di lakukan karena pada limbah tandan kosong kelapa sawit dan dedak padi merupakan sumber nutrisi yang bernilai ekonomi tinggi yang dapat dimanfaatkan sebagai media pertumbuhan jamur tiram putih (Pleurotus ostreatus). Tujuan penelitian ini adalah (1) mengetahui pertumbuhan miselium jamur tiram putih (P. ostreatus) pada substrat limbah tandan kosong kelapa sawit dan dedak padi; (2) mengetahui karakteristik pertumbuhan jamur tiram putih (P. ostreatus) pada perlakuan jenis substrat yang berbeda. Penelitian ini telah di lakukan dalam rancangan acak lengkap (RAL) yang terdiri tujuh perlakuan dan tiga kali pengulangan. Susunan perlakuan merupakan perbandingan antara tandan kosong kelapa sawit : dedak padi, yakni P1 (50% : 50%), P2 (45% : 55%), P3 (40% : 60%), P4 (35% : 65%). P5 (30% : 70%), P6 (25% : 75%), P7 (serbuk geragaji 70%, dedak padi 20%, tepung jagung 10%). Adapun hasil penelitian menunjukkan laju pertumbuhan miselium tercepat pada perlakuan P1 yaitu 89 mm dan terendah pada perlakuan P5 yaitu 80 mm. Jumlah koloni tertinggi pada perlakuan P5 yaitu 1,4x1012 CFU/g dan terendah pada perlakuan P4 yaitu 0,4x1012 CFU/g. Waktu inkubasi paling cepat pada perlakuan P4 yaitu 28 hari dan terendah pada perlakuan P7 yaitu 33 hari.","PeriodicalId":8780,"journal":{"name":"Biocelebes","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80907464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-13DOI: 10.22487/bioceb.v15i2.15782
Apri Salfiani, Asri Pirade Paserang
Provision of Vanilla (Vanilla planifolia Andrews) with traditional cultivation often has problems, including the availability of unhealthy seeds due to disease caused by Fusarium oxysporum. This disease can thwart vanilla plantations up to 85% because the pathogen can infect all parts of the vanilla plant making it difficult to control efforts. Alternative efforts were made to overcome this problem, namely through the initiation process in tissue culture by combining the hormones IAA (indole-3-acetic acid) and BAP (6-benzylaminopurine). This study aims to determine the effect and the maximum concentration of the combination of IAA and BAP on the initiation of vanilla plants. This study was conducted based on a completely randomized design (CRD) with 5 treatments and 3 repetitions. This treatment consisted of: A (MS + 0.1 ppm IAA + 1 ppm BAP), B (MS + 0.2 ppm IAA + 1 ppm BAP), C (MS + 0.3 ppm IAA + 1 ppm BAP), D (MS + 0.4 ppm IAA + 1 ppm BAP), and E (MS + 0.5 ppm IAA + 1 ppm BAP). Observations were made after 40 days from the planting process. The results showed that the concentration of the combination of IAA and BAP can affect the growth and organogenesis of the initiation of vanilla plants (Vanilla planifolia Andrews). Treatment C (MS + 0.3 ppm IAA + 1 ppm BAP) was the best concentration from this study, with average values: number of shoots (1), shoot length (1.73 cm), number of roots (1), and number of leaves (1.33).
{"title":"PENGARUH KOMBINASI IAA (Indole-3-Acetic Acid) DAN BAP (6-Benzylaminopurine) TERHADAP INISIASI TANAMAN VANILI (Vanilla planifolia Andrews)","authors":"Apri Salfiani, Asri Pirade Paserang","doi":"10.22487/bioceb.v15i2.15782","DOIUrl":"https://doi.org/10.22487/bioceb.v15i2.15782","url":null,"abstract":"Provision of Vanilla (Vanilla planifolia Andrews) with traditional cultivation often has problems, including the availability of unhealthy seeds due to disease caused by Fusarium oxysporum. This disease can thwart vanilla plantations up to 85% because the pathogen can infect all parts of the vanilla plant making it difficult to control efforts. Alternative efforts were made to overcome this problem, namely through the initiation process in tissue culture by combining the hormones IAA (indole-3-acetic acid) and BAP (6-benzylaminopurine). This study aims to determine the effect and the maximum concentration of the combination of IAA and BAP on the initiation of vanilla plants. This study was conducted based on a completely randomized design (CRD) with 5 treatments and 3 repetitions. This treatment consisted of: A (MS + 0.1 ppm IAA + 1 ppm BAP), B (MS + 0.2 ppm IAA + 1 ppm BAP), C (MS + 0.3 ppm IAA + 1 ppm BAP), D (MS + 0.4 ppm IAA + 1 ppm BAP), and E (MS + 0.5 ppm IAA + 1 ppm BAP). Observations were made after 40 days from the planting process. The results showed that the concentration of the combination of IAA and BAP can affect the growth and organogenesis of the initiation of vanilla plants (Vanilla planifolia Andrews). Treatment C (MS + 0.3 ppm IAA + 1 ppm BAP) was the best concentration from this study, with average values: number of shoots (1), shoot length (1.73 cm), number of roots (1), and number of leaves (1.33).","PeriodicalId":8780,"journal":{"name":"Biocelebes","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76384805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biofertilizer can be used to replace chemical fertilizer so that soil quality is maintained and soil pollution can be avoided. The study aimed to determine the effect of biofertilizers with different compositions of microorganisms on the growth of maize plants. The research was carried out in a greenhouse. Maize plants were fertilized by two kinds of biofertilizers with different compositions of microorganisms. The first biofertilizer contained Azotobacter sp, Azospirillum sp, Rhizobium sp, Trichoderma sp, and Lactobacillus sp, and the second biofertilizer contained Strenotrophomonas sp. and Paenibacillus polymyxa. As controls, maize plants were fertilized by sterilized those biofertilizers. The results showed that the second biofertilizer increased the dry weight of the maize plant. Meanwhile, the first biofertilizer did not increase it. Several things must be considered in the application of biofertilizers, such as the composition of microorganisms, type of plant, level of fertilization, and the method of applying fertilizer to the plant.
{"title":"PENGARUH PUPUK HAYATI DENGAN KOMPOSISI MIKROORGANISME YANG BERBEDA TERHADAP PERTUMBUHAN TANAMAN","authors":"Amalia Puji` Cahyani, Asri Pirade Paserang, Meryany Ananda, Wahyu Harso","doi":"10.22487/bioceb.v15i2.15781","DOIUrl":"https://doi.org/10.22487/bioceb.v15i2.15781","url":null,"abstract":"Biofertilizer can be used to replace chemical fertilizer so that soil quality is maintained and soil pollution can be avoided. The study aimed to determine the effect of biofertilizers with different compositions of microorganisms on the growth of maize plants. The research was carried out in a greenhouse. Maize plants were fertilized by two kinds of biofertilizers with different compositions of microorganisms. The first biofertilizer contained Azotobacter sp, Azospirillum sp, Rhizobium sp, Trichoderma sp, and Lactobacillus sp, and the second biofertilizer contained Strenotrophomonas sp. and Paenibacillus polymyxa. As controls, maize plants were fertilized by sterilized those biofertilizers. The results showed that the second biofertilizer increased the dry weight of the maize plant. Meanwhile, the first biofertilizer did not increase it. Several things must be considered in the application of biofertilizers, such as the composition of microorganisms, type of plant, level of fertilization, and the method of applying fertilizer to the plant.","PeriodicalId":8780,"journal":{"name":"Biocelebes","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90073210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-13DOI: 10.22487/bioceb.v15i2.15654
S. Enal, Amirudin Kasim, Musdalifah Nurdin, Andi Tandra Tellu
The purpose of this study was to obtain information about the types of plants used by the Kaili Da’a tribe in life cycle ceremonies that are still preserved for generations in Uwemanje village, Kinovaro district, Sigi district. The research population is all people who live in Uwemanje Village, Kinivaro District, which are spread over 4 hamlets. The research method uses survey research with the help of interview questionnaires. Interviews were conducted on key respondents and general respondents. Key respondents were selected by purposive sampling as much as 10% of the population, namely the people who were considered to be the most knowledgeable about the ins and outs of this ceremony, such as Sando, traditional leaders, village heads and village priests. Meanwhile, general respondents are people who are recommended by key respondents with a record that they are at least 30 years old and are married or have a family. The data and information in this study were processed descriptively. Types of plants/plants used in life cycle ceremonies consist of 21 species, namely: rice (Oryza sativa L.), coconut (Cocos nucifera L.), banana (Musa paradisiaca L.), cocor duck (Kalanchoe pinata L.), areca nut (Areca catechu L.), turmeric (Curcuma domestica L.), nail paci (Lawsonia enermis L.), bogenvil (Boganvillea glabra L.), pandanus (Pandanus amarylifolius L.), jatropha (Jatropha curcas), forest basil ( Ocimum sanctum L.), moringa (Moringa oleifera L.), shallot (Allium cepa L.), Bengal grass (Eleusine indica L.), hibiscus (Hibiscus tiliaceus L.), peacock flower (Caesalpinia pulcherima L.), betel (Piper betle L.), keluwih (Artocarpus camansi L.), jasmine (Jasminum sambac L.), palm sugar (Arenga pinnata), and red sugar cane (Saccharum officinarum L.). The percentage of utilization of plant parts that are most directly utilized is the leaves which amount to 14 with a percentage of 65.66%, flowers amount to 4 to 16.67%, fruit to reach 3 to 12.5%. The least used parts are rhizomes, seeds, tubers, roots and stems with a percentage of 5.17%.
{"title":"IDENTIFICATION OF PLANT UTILIZATION IN THE LIFE CYCLE CEREMONY OF THE KAILI DA'A TRIBE IN UWEMANJE VILLAGE, KINOVARO DISTRICT, SIGI REGENCY","authors":"S. Enal, Amirudin Kasim, Musdalifah Nurdin, Andi Tandra Tellu","doi":"10.22487/bioceb.v15i2.15654","DOIUrl":"https://doi.org/10.22487/bioceb.v15i2.15654","url":null,"abstract":"The purpose of this study was to obtain information about the types of plants used by the Kaili Da’a tribe in life cycle ceremonies that are still preserved for generations in Uwemanje village, Kinovaro district, Sigi district. The research population is all people who live in Uwemanje Village, Kinivaro District, which are spread over 4 hamlets. The research method uses survey research with the help of interview questionnaires. Interviews were conducted on key respondents and general respondents. Key respondents were selected by purposive sampling as much as 10% of the population, namely the people who were considered to be the most knowledgeable about the ins and outs of this ceremony, such as Sando, traditional leaders, village heads and village priests. Meanwhile, general respondents are people who are recommended by key respondents with a record that they are at least 30 years old and are married or have a family. The data and information in this study were processed descriptively. Types of plants/plants used in life cycle ceremonies consist of 21 species, namely: rice (Oryza sativa L.), coconut (Cocos nucifera L.), banana (Musa paradisiaca L.), cocor duck (Kalanchoe pinata L.), areca nut (Areca catechu L.), turmeric (Curcuma domestica L.), nail paci (Lawsonia enermis L.), bogenvil (Boganvillea glabra L.), pandanus (Pandanus amarylifolius L.), jatropha (Jatropha curcas), forest basil ( Ocimum sanctum L.), moringa (Moringa oleifera L.), shallot (Allium cepa L.), Bengal grass (Eleusine indica L.), hibiscus (Hibiscus tiliaceus L.), peacock flower (Caesalpinia pulcherima L.), betel (Piper betle L.), keluwih (Artocarpus camansi L.), jasmine (Jasminum sambac L.), palm sugar (Arenga pinnata), and red sugar cane (Saccharum officinarum L.). The percentage of utilization of plant parts that are most directly utilized is the leaves which amount to 14 with a percentage of 65.66%, flowers amount to 4 to 16.67%, fruit to reach 3 to 12.5%. The least used parts are rhizomes, seeds, tubers, roots and stems with a percentage of 5.17%.","PeriodicalId":8780,"journal":{"name":"Biocelebes","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90118760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-13DOI: 10.22487/bioceb.v15i2.15777
M. Guli
The immune sistem is a way of the body’s defense sistem to save the host from the invasion of outside pathogen. Based on how respon to disease, that differentiated into two immune system are innate and adaptive system. Because it an cant throgh the stomach, these pathogenic bacteria go to the small intestin as a site infection. In the intestine, V. cholerae bactesia adhere and colonize and invasion to intestinal epihelial cells. Protection mechanism to V. cholerae are the natural defense presence of tick mucosa on the surface of epithelial cells can inhibit pathogene to adhere tointestinal epithelial cells. One anothet defense namely innate immune system did by phagocytic cells to attac pathogen agent and adaptive immune system involves IgA to opsonization so that can increase intestinal mucosal immune system
{"title":"RESPON IMUN HOSPES TERHADAP INFEKSI Vibrio cholerae","authors":"M. Guli","doi":"10.22487/bioceb.v15i2.15777","DOIUrl":"https://doi.org/10.22487/bioceb.v15i2.15777","url":null,"abstract":"The immune sistem is a way of the body’s defense sistem to save the host from the invasion of outside pathogen. Based on how respon to disease, that differentiated into two immune system are innate and adaptive system. Because it an cant throgh the stomach, these pathogenic bacteria go to the small intestin as a site infection. In the intestine, V. cholerae bactesia adhere and colonize and invasion to intestinal epihelial cells. Protection mechanism to V. cholerae are the natural defense presence of tick mucosa on the surface of epithelial cells can inhibit pathogene to adhere tointestinal epithelial cells. One anothet defense namely innate immune system did by phagocytic cells to attac pathogen agent and adaptive immune system involves IgA to opsonization so that can increase intestinal mucosal immune system","PeriodicalId":8780,"journal":{"name":"Biocelebes","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86029358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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