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Production of mycelium-based biomaterial using various substrates 利用各种基质生产菌丝体基生物材料
Pub Date : 2023-09-10 DOI: 10.6001/biologija.2023.69.3.3
Sanmugavel Saravana, Irma Ražanskė, Vykintas Baublys, Vaida Tubelytė
This experiment was carried out to create a biomaterial with mycelium as its main component and binding part. The main object of this research was mycelium of Pleurotus ostreatus. Various substrates were used for mycelium growing: buckwheat husks, onion husks, garlic husks, coconut husk substrate, coffee grounds, birch sawdust, wheat grains, ground wheat, hemp husks, rock wool, and polystyrene. These different substrates and materials were mixed with the mycelium culture and grown for at least three months, with growth monitored weekly. The blocks of grown biomaterial were dried and tested for properties such as water absorption, moisture content, and biodegradation. The conducted studies showed that mycelium can successfully colonise and grow on different substrates, forming biomaterials with different properties. The choice of substrate influenced the physical characteristics of the obtained biomaterials. Mycelium can be used as a sustainable alternative for the production of various biomaterials. The results of this study add to the knowledge in the field of mycelium-based biomaterials and open the way for further research and development of this promising technology.
本实验旨在制备一种以菌丝为主要成分和结合部分的生物材料。本研究以平菇菌丝体为主要研究对象。用于菌丝体生长的基质有:荞麦壳、洋葱壳、大蒜壳、椰子壳、咖啡渣、桦木锯末、小麦粒、磨碎的小麦、大麻壳、岩棉和聚苯乙烯。将这些不同的基质和材料与菌丝培养液混合,培养至少三个月,每周监测生长情况。将生长的生物材料块干燥并测试其吸水性、含水率和生物降解性等性能。所进行的研究表明,菌丝体可以成功地在不同的基质上定植和生长,形成具有不同性质的生物材料。底物的选择影响所得生物材料的物理特性。菌丝体可以作为一种可持续的替代品,用于生产各种生物材料。本研究的结果增加了菌丝体生物材料领域的知识,并为进一步研究和开发这一有前途的技术开辟了道路。
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引用次数: 0
Strategies to alleviate the unusual effects of climate change on crop production: a thirsty and warm future, low crop quality. A review 缓解气候变化对作物生产异常影响的策略:干旱和温暖的未来,低质量的作物。回顾
Pub Date : 2023-06-27 DOI: 10.6001/biologija.2023.69.2.1
M. Janmohammadi, N. Sabaghnia
Climate change is one of the most common challenges in semiarid regions, which are affected by such constraints as drought, heat stress, and decreased soil fertility. The most important consequences of climate change – rising temperature, decreasing rainfall, and deteriorating quality of some crops – are studied in detail in this review. The main future climate scenarios in semiarid regions include decreased precipitation with highly irregular and unpredictable distribution patterns, with most rainfall concentrated over winter months, prolonged dry spells, early heat stress, exacerbation of edaphic constraints such as reduced soil moisture content, reduced soil organic matter, reduced soil biodiversity, reduced critical nutrient availability, and, ultimately, fewer crop yields. Due to the moisture and soil constraints caused by climate change, the cropping system, crop succession, and soil tillage techniques will need a major overhaul, and this requires modifying soil management and fertilisation, design of some new agro-climatic zoning, including neglected and underutilised crop species in rotations, optimised sowing time windows, expanded irrigation, design of multi-functional cropping systems, conservation agriculture, some changes in agricultural equipment, and the use of climate-smart agriculture practices. Rising carbon dioxide concentrations will decrease nitrogen metabolism by reducing photorespiration, and this reduces the protein content of the grain and worsens the quality of the staple crops. Selection of climate-smart varieties, application of agro-inputs at the right time, right place, and right amount (precision agriculture), and the use of leading technologies such as nano for designing the smart fertiliser should be considered to overcome the adverse effects of climate anomalies.
气候变化是半干旱地区最常见的挑战之一,这些地区受到干旱、热应激和土壤肥力下降等制约因素的影响。本文详细研究了气候变化最重要的后果——气温上升、降雨量减少和某些作物品质恶化。未来半干旱地区的主要气候情景包括降水减少,且分布模式高度不规则和不可预测,大部分降雨集中在冬季,干旱期延长,热胁迫提前,土壤水分含量降低、土壤有机质减少、土壤生物多样性减少、关键养分有效性降低等土壤约束加剧,最终导致作物产量下降。由于气候变化造成的水分和土壤限制,种植制度、作物演代和土壤耕作技术将需要进行重大改革,这需要修改土壤管理和施肥,设计一些新的农业气候区划,包括轮作中被忽视和未充分利用的作物品种,优化播种时间窗口,扩大灌溉,设计多功能种植系统,保护性农业,对农业设备进行一些改变,以及采用气候智能型农业做法。二氧化碳浓度升高会通过减少光呼吸作用而降低氮素代谢,从而降低籽粒蛋白质含量,使主粮作物品质恶化。为了克服气候异常的不利影响,应该考虑选择气候智能型品种,在正确的时间、地点和数量上应用农业投入物(精准农业),以及使用纳米等领先技术来设计智能肥料。
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引用次数: 0
The second heat wave leads to changes in the efficiency of alfalfa’s photosynthetic energy use 第二次热浪导致紫花苜蓿光合能量利用效率的变化
Pub Date : 2023-06-27 DOI: 10.6001/biologija.2023.69.2.2
I. Januškaitienė
Extreme climatic events, which have become more common, more intense, and more frequent, threatencrop productivity and food security. The aim of this study was to investigate the response of chlorophyll a fluorescence parameters of Medicago sativa L. under regulated environment and recurrent heat waves stress effect. Plants were grown in pots. Two four-day heat waves (35/28°C day/night temperature) were simulated simultaneously with drought (10% soil moisture). Each four-day heat wave was followed by a five-day recovery period. Measurements of chlorophyll a fluorescence parameters were taken on the last, fourth, day of the exposure of each heat wave and after each recovery period. Results of this study showed that both the first and second heat waves significantly reduced the quantum yield of PSII photochemistry. During the second heat wave, the negative effects persisted, but were already weaker. The same pattern of change wasfound for the performance index (PIABS). PIABS decreased by 54.0% and 46.8% during the first and second heat waves, respectively, compared to controls (p < 0.05). Despite the increase in absorbed and trapped energy due to the effect of the heat waves, the electron transport rate from QA to QB in the plants exposed to heat waves was not followed by an increase. The above-mentioned changes in the viability of the photosystem may have been caused by a decrease in the density of the active reaction centers (RC/CSo) and an increase in the amount of dissociated energy (DIo/CSo). After the second recovery period, the RC/CSo in heat and drought stressed plants was equal to that of control plants, which resulted in lower energy waste in the form of heat (DIo/CSo).
极端气候事件变得更加普遍、更加剧烈和频繁,威胁着生产力和粮食安全。研究了紫花苜蓿(Medicago sativa L.)叶绿素a荧光参数在调控环境和反复热浪胁迫下的响应。植物在花盆里种植。同时模拟了两个为期四天的热浪(35/28°C昼夜温度)和干旱(10%土壤湿度)。每四天的热浪之后是五天的恢复期。在每个热浪暴露的最后、第4天和每个恢复期后测量叶绿素a荧光参数。研究结果表明,第一次和第二次热波均显著降低了PSII光化学的量子产率。在第二次热浪中,负面影响持续存在,但已经减弱。在绩效指数(PIABS)中也发现了同样的变化模式。在第一次和第二次热浪中,PIABS分别比对照组下降了54.0%和46.8% (p < 0.05)。尽管由于热浪的影响,吸收和捕获的能量有所增加,但暴露在热浪下的植物从QA到QB的电子传递率并没有随之增加。上述光系统活力的变化可能是由于活性反应中心(RC/CSo)密度的降低和解离能(DIo/CSo)的增加引起的。第2个恢复期后,热干旱胁迫植物的RC/CSo与对照植物相当,导致热量形式的能量浪费(DIo/CSo)减少。
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引用次数: 0
The impact of sex, age, and season on the prevalence of Helicobacter pylori infection in Lithuania 性别、年龄和季节对立陶宛幽门螺杆菌感染率的影响
Pub Date : 2023-06-27 DOI: 10.6001/biologija.2023.69.2.4
I. Lipatova, Ieva Kulnickaitė, Živilė Jacikė, Ieva Korsakovė
The aim of this study was to identify the prevalence of H. pylori infection in adult patients in Kaunas, Lithuania, and to assess the dependence of H. pylori infection on sex, age, and seasonality. A total of 5799 patients were tested for the presence of H. pylori by ELISA. Three different tests were used: IgA and IgG antibodies against H. pylori from blood serum and antigens of H. pylori from faeces. The results revealed that the frequency of H. pylori infection varied depending on the test (31.8% for IgG, 16.7% for IgA, and 22.9% for antigens test). No significant association between sex, season, and the prevalence of H. pylori was observed. However, our findings indicate a significant increase in the prevalence of H. pylori infection with age. This study is the first report about the impact of sex, age, and season on the prevalence of H. pylori infection in Lithuania.
本研究的目的是确定立陶宛考纳斯成年患者幽门螺杆菌感染的患病率,并评估幽门螺杆菌感染对性别、年龄和季节性的依赖。采用ELISA法对5799例患者进行幽门螺杆菌检测。采用三种不同的检测方法:血清中针对幽门螺杆菌的IgA和IgG抗体和粪便中幽门螺杆菌抗原。结果显示,不同检测方法幽门螺杆菌感染的频率不同(IgG检测31.8%,IgA检测16.7%,抗原检测22.9%)。性别、季节和幽门螺杆菌患病率之间没有明显的联系。然而,我们的研究结果表明,幽门螺杆菌感染的患病率随着年龄的增长而显著增加。这项研究是关于立陶宛性别、年龄和季节对幽门螺杆菌感染流行率影响的第一份报告。
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引用次数: 0
Influence of organic agricultural production on the condition of surface water 有机农业生产对地表水状况的影响
Pub Date : 2023-06-27 DOI: 10.6001/biologija.2023.69.2.3
Rasa Žolynaitė, S. Pocius, L. Česonienė
Agriculture and its methods of operation constantly influence the quality of water of surface water bodies. It was established in 2021 that in Lithuania, 63% of water bodies of the river and 64% of the lake categories are classified as risk water bodies and do not meet the good condition criteria. One of the main factors leading to the deterioration of the condition of surface water bodies is the continuing and increasing diffuse pollution, mainly from agricultural activities, together with pollution from agriculture; also, the condition in some water bodies deteriorates because of concentrated pollution, persistent effects of hydromorphological changes in surface water bodies, natural processes, and changing climatic conditions. This study aimed to determine the degree at which the ecological method of agricultural production affects the nearby surface water bodies. The obtained results show that the larger the area of organic farms from the entire area of the nutrient basin of a lake, the lower the values of biochemical oxygen consumption, total phosphorus, phosphate phosphorus, nitrate nitrogen, and total nitrogen concentrations. The ecological method of agricultural production has a positive effect on the condition of surface water bodies.
农业及其经营方式不断地影响着地表水的水质。立陶宛于2021年确定,63%的河流水体和64%的湖泊类别被列为风险水体,不符合良好条件标准。导致地表水状况恶化的主要因素之一是持续和增加的以农业活动为主的弥漫性污染,以及农业污染;此外,由于污染的集中、地表水形态变化的持续影响、自然过程和气候条件的变化,一些水体的状况恶化。本研究旨在确定生态农业生产方式对附近地表水水体的影响程度。结果表明:湖泊营养源流域面积越大,生化耗氧量、总磷、磷酸磷、硝态氮和总氮浓度越低;生态农业生产方式对地表水水体状况有积极影响。
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引用次数: 0
Effect of rainfall on the structure and population densities of birds breeding in a suburb of Windhoek, Namibia 降雨对纳米比亚温得和克郊区鸟类繁殖结构和种群密度的影响
Pub Date : 2023-03-23 DOI: 10.6001/biologija.2023.69.1.1
G. Kopij
A bird community was quantified in the suburb of Olympia (200 ha) in the city of Windhoek, Namibia. Two years (2014 and 2020) were selected to examine the impact of rainfall and sub- urb development on species diversity and population densities of the selected bird species in this community. The total amount of rainfall in Windhoek in 2014 was much higher than in 2020 (475 mm and 275 mm, respectively). In total, 36 breeding bird species were recorded in the study area in 2014 and 2020: 27 spe- cies in 2014 and 31 species in 2020. The number of species com- mon in 2014 and 2020 was 17. The Sörensen’s Similarity Index was therefore rather low (0.59). In overall, population density of 25 selected bird species was much higher in 2020 than in 2014. Population densities of the Laughing Dove, the Southern Masked Weaver, and Chestnut-vented Warbler in 2014 and 2020 were statistically different, whereas population densities of the Red- headed Finch were similar in both years compared. The breeding bird community in Olympia suburb does not ap- pear to be stable over years, both in regard to species composition and population densities of particular species. This instability may be linked to constant property development of this suburb and around it. Olympia suburb borders on the periphery of the city, which is a savanna slowly transformed into another suburb. Un- der drought conditions, some species from these surroundings may be attracted to the suburbs by water and food. Hence, con- trary to expectation, densities of some species in the suburbs in the year with low rainfall are higher.
在纳米比亚温得和克市奥林匹亚郊区(200公顷)对一个鸟类群落进行了量化。选择2014年和2020年,研究了降雨和城郊发展对该群落鸟类物种多样性和种群密度的影响。2014年温得和克的总降雨量远高于2020年(分别为475毫米和275毫米)。2014年和2020年研究区共记录到36种繁殖鸟类,2014年27种,2020年31种。2014年和2020年共有种数为17种。因此,Sörensen的相似指数很低(0.59)。总体而言,2020年25种鸟类种群密度明显高于2014年。2014年和2020年,笑鸽、南方蒙面织布鸟和板栗莺的种群密度有统计学差异,而红头雀的种群密度在2014年和2020年是相似的。奥林匹亚郊区的繁殖鸟类群落在物种组成和特定物种的种群密度方面,多年来似乎并不稳定。这种不稳定可能与这个郊区及其周围不断发展的房地产有关。奥林匹亚郊区毗邻城市的外围,这是一片稀树草原慢慢转变成另一个郊区。在干旱条件下,这些环境中的一些物种可能会被水和食物吸引到郊区。因此,与预期相反,在降雨量少的年份,郊区某些物种的密度更高。
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引用次数: 0
Monitoring of training activity as a determinant of people’s health in the COVID-19 pandemic crisis 监测培训活动,将其作为COVID-19大流行危机中人们健康的决定因素
Pub Date : 2023-03-23 DOI: 10.6001/biologija.2023.69.1.2
Olena Tomarevska, Z. Boiarska, O. Poliakov
Reliable determination of metabolic and physical activity pa- rameters and influences of blood pressure indicators is relevant in the development of rehabilitation training programmes for people of all ages, especially in the context of the COVID-19 pan- demic and the aging of the working population. The purpose of the study was to determine reliable parameters that were shaping general health during the COVID-19 pandemic crisis in the age- related aspect. This study used the results of comprehensive health monitoring data of 603 adults aged 20–89 from the Human Health Passport online tool (Ukraine) for the period of 2020–2022. On- line health monitoring showed the consequences of mild forms of COVID-19 disease. The main indicators informing about this effect were the laboratory-method indicator for confirming a viral disease and the number of cases of mild forms of the dis- ease. The indirect influence of this factor on various indicators of health, physical activity, metabolism, indicators of blood pressure compensation should also be considered. As for the age aspect, the state of health had a higher influence on the indicators of body mass index (BMI), blood pressure and its correction with drugs, the daily walking distance and exercises performed, and a danger- ous loss of appetite. A noticeable drop in body weight and loss of appetite are reliable factors in the incidence of falls and decline in health. The leading factors shaping the health of the active popu- lation in mild forms of COVID-19 are physical activity (7.77%), metabolism (5.91%), and the blood pressure level (5.21%).
可靠地确定代谢和体力活动参数以及血压指标的影响,对于制定针对所有年龄段人群的康复训练方案具有重要意义,特别是在2019冠状病毒病大流行和劳动人口老龄化的背景下。本研究的目的是确定在COVID-19大流行危机期间与年龄相关方面影响总体健康状况的可靠参数。本研究使用了2020-2022年期间603名20-89岁成年人的综合健康监测数据,这些数据来自人类健康护照在线工具(乌克兰)。在线健康监测显示了轻度COVID-19疾病的后果。反映这种影响的主要指标是确认病毒性疾病的实验室方法指标和轻度疾病的病例数。还应考虑这一因素对健康、体力活动、新陈代谢、血压代偿等各项指标的间接影响。至于年龄方面,健康状况对身体质量指数(BMI)、血压及其药物校正、每日步行距离和锻炼以及食欲危险下降等指标的影响较大。体重明显下降和食欲不振是导致跌倒和健康状况下降的可靠因素。影响轻度COVID-19活跃人群健康状况的主要因素是身体活动(7.77%)、新陈代谢(5.91%)和血压水平(5.21%)。
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引用次数: 0
Composition, distribution, and diversity of macrophytes and benthic macroinvertebrate communities in Eleyele Lake, southwestern Nigeria 尼日利亚西南部Eleyele湖大型植物和底栖大型无脊椎动物群落的组成、分布和多样性
Pub Date : 2022-12-13 DOI: 10.6001/biologija.v68i4.4836
Adedolapo Abeke Ayoade, Halimah Adewumi Adeyemi
Many human activities that have an effect on water quality and aquatic health are increasing along Eleyele Lake, which is a vital resource for domestic water supply and fishery in Ibadan, southwest Nigeria. This study provides information on the composition of macrophytes and benthic macroinvertebrates (which are bioindicators) in association with physicochemical parameters in Eleyele Lake. Water, macrophytes, and benthic macroinvertebrates were collected from four stations monthly from March to August 2021 and analysed using standard methods. The relationship between the measured physicochemical variables, macrophytes, and the abundance of benthic macroinvertebrate was explored using canonical correspondence analysis (CCA). The encountered macrophytes represented 19 species (11 emergent, six floating and two submerged) in 16 genera and 12 families. The most abundant species was Ipomea aquatica (25.83%) in station 2 and Sacciolepsis africana (19.19%) in station 1. A total of 282 benthic macroinvertebrate individuals belonging to three phyla, four classes, 11 genera, and 11 species were encountered. Pollution indicator species including Melanoides tuberculata, Biomphalaria pfeifferi and Chironomus larvae observed in all the sampling stations were the dominant species. The CCA indicated that the abundance of macrophytes correlated with depth and nutrient factors (mostly phosphate). The result of the CCA showed the abundance of Chironomus larvae being patterned by dissolved oxygen and nutrient factors (phosphate, nitrate, and sulphate). These further confirmed the polluted status of Eleyele Lake, especially by nutrient enrichment. The dominant macrophytes and benthic macroinvertebrates in Eleyele Lake are pollution-tolerant species and the lake has been impacted by nutrient enrichment.
Eleyele湖是尼日利亚西南部伊巴丹市家庭供水和渔业的重要资源,在Eleyele湖沿岸,对水质和水生健康产生影响的许多人类活动正在增加。本研究提供了Eleyele湖大型植物和底栖大型无脊椎动物组成(生物指标)与理化参数的关系。从2021年3月至8月,每月从四个站点收集水、大型植物和底栖大型无脊椎动物,并使用标准方法进行分析。利用典型对应分析(canonical correspondence analysis, CCA)探讨了测量的理化变量、大型植物与底栖大型无脊椎动物丰度之间的关系。所发现的大型植物包括12科16属19种(11种是水生植物,6种是浮生植物,2种是水下植物)。2号站和1号站分别以水蜜桃(25.83%)和非洲Sacciolepsis(19.19%)丰富。共捕获大型底栖无脊椎动物282只,隶属于3门4纲11属11种。各监测站的污染指示种均为优势种,其中结核黑素、菲费生物phalaria和Chironomus幼虫为优势种。CCA表明,植物丰度与深度和养分因子(主要是磷酸盐)相关。CCA的结果表明,螯合虫幼虫的丰度受溶解氧和营养因子(磷酸盐、硝酸盐和硫酸盐)的影响。这些进一步证实了eleyelle湖的污染状况,特别是营养物的富集。Eleyele湖的优势大型植物和底栖大型无脊椎动物是耐污染的物种,湖泊受到营养物富集的影响。
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引用次数: 1
Assessment of the microbial contamination of stairwell door handles in blocks of flats 公寓楼楼梯间门把手的微生物污染评估
Pub Date : 2022-12-13 DOI: 10.6001/biologija.v68i4.4837
Žaneta Maželienė, Deimantė Rutkauskaitė, A. Aleksandravičienė
The stairwell door handles in blocks of flats are the objects that every resident and guests touch at least several times a day. For this reason, many microorganisms collect on them, which can cause infections of varying severity. Therefore, it is very important to observe proper hand hygiene and disinfection of frequently touched surfaces on the stairwells of blocks of flats. Research samples were taken in the months of October-November in 2021 and December-January in 2022, in the morning (between 7:00 and 8:00) and in the evening (between 19:00 and 20:00), from the stairwells of the blocks of flats in the city of Kaunas. One-hundred samples were taken in the autumn season (50 in the morning and 50 in the evening) and 100 samples in the winter season (50 in the morning and 50 in the evening). The research sample consisted of 200 samples; four analytes were analysed in each sample: Enteroccocus spp., Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus. The stairwell door handles of multi-apartment buildings were contaminated with Enterococcus spp. the most and with S. aureus bacteria the least. Slightly higher microbial contamination of multi-apartment stairwells was observed in the evening. Microbial contamination of multi-apartment stairwell door handles was observed to be higher in the autumn season than in the winter season.
公寓楼里的楼梯井门把手是每个住户和客人每天至少要碰几次的东西。由于这个原因,许多微生物聚集在它们上面,这可能导致不同程度的感染。因此,保持适当的手卫生,并对经常接触的楼梯间表面进行消毒,是非常重要的。研究样本于2021年10月至11月和2022年12月至1月,分别于早上(7:00至8:00)和晚上(19:00至20:00)在考纳斯市公寓楼的楼梯井中采集。秋季采集100份样本(早上50份,晚上50份),冬季采集100份样本(早上50份,晚上50份)。研究样本由200个样本组成;每个样品分析了四种分析物:肠球菌、铜绿假单胞菌、大肠杆菌和金黄色葡萄球菌。居民楼楼梯间门把手受肠球菌污染最多,金黄色葡萄球菌污染最少。多套公寓楼梯间的微生物污染在晚间稍高。多套公寓楼梯间门把手的微生物污染在秋季高于冬季。
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引用次数: 0
An in silico study into the bioacaricide power of the Algerian argan tree against Varroa destructor 阿尔及利亚摩洛哥坚果树对瓦螨的生物杀螨力的计算机研究
Pub Date : 2022-12-13 DOI: 10.6001/biologija.v68i4.4839
Amina Belhadji, I. Abdelli, F. Hassani, Sohayb Bekkal Brikci, Sarra Ghalem, Hafiz Muzzammel Rehman, Réda Kechairi
Varroa destructor is the parasitic mite of the honeybee, Apis mellifera. It presents a major threat to the health of bees and to the quality and quantity of honey. Varroa destructor have increased their resistance to acaricides; consequently, the mites or their vector viruses become more virulent. Infested colonies, commonly referred to as ‘mite bombs’, facilitate the dispersal of mites and transmission of disease to stronger and healthier colonies. Acaricides are the most used means of control, although the use of these chemical products has a negative impact on the health of bees, the quality of honey, human health, and the environment. The argan tree of the genus Argania is a tropical tree of the Sapotaceae family. This plant is very important economically. The argan tree is the source of biologically active and edible oil. In this context, we aim to test the bioacaricide power of the argan tree to fight the V. destructor by molecular modelling methods.
瓦螨是蜜蜂的寄生螨。它对蜜蜂的健康以及蜂蜜的质量和数量构成重大威胁。瓦roa破坏者提高了对杀螨剂的抵抗力;因此,螨虫或它们的载体病毒变得更有毒性。被感染的蜂群,通常被称为“螨虫炸弹”,有助于螨虫的扩散和疾病的传播,使蜂群更强壮、更健康。杀螨剂是最常用的控制手段,尽管这些化学产品的使用对蜜蜂的健康、蜂蜜的质量、人类健康和环境都有负面影响。摩洛哥坚果树属的摩洛哥坚果树是仙人掌科的一种热带树木。这种植物在经济上很重要。摩洛哥坚果树是生物活性和食用油的来源。在这种情况下,我们的目的是通过分子模拟方法来测试摩洛哥坚果树的生物杀螨能力。
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引用次数: 0
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Biologija
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