Biological Control最新文献

{"title":"Co-occurrence of entomopathogenic nematodes and earthworms enhances enduring biocontrol activity and microbial diversity in a naturalized plant-soil system","authors":"Maryam Chelkha ,&nbsp;Rubén Blanco-Pérez ,&nbsp;David Labarga ,&nbsp;María de Toro ,&nbsp;Jorge Dueñas-Hernani ,&nbsp;Kyle Wickings ,&nbsp;Raquel Campos-Herrera","doi":"10.1016/j.biocontrol.2024.105685","DOIUrl":"10.1016/j.biocontrol.2024.105685","url":null,"abstract":"<div><div>Soil ecosystems host diverse microorganisms and fauna essential for terrestrial processes, with earthworms (EWs) and entomopathogenic nematodes (EPNs) playing crucial roles. EWs enhance soil health by improving aeration, porosity, and nutrient cycling, while EPNs, such as <em>Steinernema</em> and <em>Heterorhabditis</em>, manage pests by killing insects. This study aimed to assess the impact of EWs and their derivatives (cutaneous excreta, CEx), alone or combined with EPNs, on soil–plant dynamics, hypothesizing that their co-occurrence would alter soil properties, bacterial communities, EPN virulence, and plant performance. Using tomato plants and field soil, the study investigated different treatments: control, EW (<em>Eisenia fetida</em>), EPN (<em>Steinernema feltiae</em>), CEx, and combinations of EPN-EW and EPN-CEx, at two and four weeks post-application. Assessments included plant growth, EPN infectivity, soil properties, and bacterial profiling via 16S rRNA gene sequencing. Results showed no significant impact on plant growth. However, EPN virulence decreased after 30 days when applied alone but was maintained or enhanced when combined with EW or CEx. Combined applications of EPNs and CEx reduced Mg and Ca contents, while organic matter increased in the EPN-EW treatment. Bacterial community changes were observed 30 days post-inoculation, with increased alpha diversity in co-applications of EPNs and EWs. The co-application of EPNs and EWs resulted in beneficial impacts on soil properties, EPN virulence, and bacterial diversity. Timing post-inoculation was crucial in assessing these effects, only detecting those changes after 30 days, suggesting the need for further extended research to understand the duration of these changes. This study highlights the intricate interactions between EWs, EPNs, and plant-soil systems, emphasizing their potential impact on plant growth, soil nutrient dynamics, and soil organisms, highlighting the importance of timing in evaluating these interactions.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"200 ","pages":"Article 105685"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143181295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One size does not fit all: Classical weed biological control across continents","authors":"Emily J. Le Falchier ,&nbsp;T. Telmadarrehei ,&nbsp;M.A. Rafter ,&nbsp;C.R. Minteer","doi":"10.1016/j.biocontrol.2024.105661","DOIUrl":"10.1016/j.biocontrol.2024.105661","url":null,"abstract":"<div><div>Pre-release quarantine testing of weed biological control agents is frequently initiated using simple no-choice tests. However, these fundamental host range tests are conservative and often do not realistically depict how potential biological control agents might interact with surrounding non-target plants in the field. If no-choice tests are the only method employed it can lead to the rejection of sufficiently host-specific agents. Ecological host range studies in areas where the biological control agent is native or already present can test the validity of laboratory findings with the advantage of evaluating long-range host-recognition behaviors of the insect. A weed biological control program targeting <em>Schinus terebinthifolia</em> Raddi (Anacardiaceae) was given permission to release <em>Pseudophilothrips ichini</em> (Hood) (Thysanoptera: Phlaeothripidae) in Florida, USA in 2019. Information from the program in the USA has provided insight for other countries with invasive Brazilian peppertree, such as Australia, which has several native non-target species within the Anacardiaceae family. To evaluate the risk of non-target attack by <em>P. ichini</em> on three Australian native non-target species (<em>Rhus taitensis</em>, <em>Pleiogynium timoriense</em>, and <em>Euroschinus falcatus</em>), we performed a two-phase open-field experiment to simulate the spillover risk of the biological control agent in both the presence and absence of their preferred host, <em>S. terebinthifolia</em>. Also included in the open-field experiment were <em>Schinus molle</em>, a close relative of <em>S. terebinthifolia</em> introduced in the USA and Australia, and the Hawaii native <em>Rhus sandwicensis.</em> The results suggest that <em>P. ichini</em> populations may spill over onto two non-target plants, <em>S. molle</em> and <em>R</em>. <em>taitensis</em>, in the absence of the target weed <em>S. terebinthifolia,</em> and could pose a risk to this native species in Australia. This work highlights the importance of international collaborations on invasive plant biocontrol programs.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"200 ","pages":"Article 105661"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143181461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Susceptibility of European Agrilus beetle species to the biocontrol agents of Emerald Ash Borer in the laboratory","authors":"Katy Reed ,&nbsp;Freya Cole ,&nbsp;Neil Audsley ,&nbsp;Anastasia Uglow ,&nbsp;Rachel Down ,&nbsp;Kerry Barnard ,&nbsp;Daegan Inward","doi":"10.1016/j.biocontrol.2024.105678","DOIUrl":"10.1016/j.biocontrol.2024.105678","url":null,"abstract":"<div><div>The emerald ash borer (EAB, <em>Agrilus planipennis</em> Fairmaire (Coleoptera: Buprestidae)<!--> <!-->is devastating ash trees in its invaded range of North America and spreading rapidly towards Western Europe from European Russia and Ukraine. To allow a rapid response when the beetle is detected, slow its spread and protect trees as part of a wider integrated pest management programme, pre-emptive biological control strategies that are suitable for Great Britain and the rest of Europe must urgently be developed. Three classical hymenopteran biological control agents have been mass-reared and released within North America to control EAB: the egg parasitoid <em>Oobius agrili</em> Zhang and Huang (Hymenoptera: Encyrtidae), and the larval parasitoids <em>Tetrastichus planipennisi</em> and <em>Spathius galinae</em> Belokobylskij &amp; Strazanac (Hymenoptera: Braconidae)<em>.</em> This study presents data on the risk these parasitoid wasps might pose to British <em>Agrilus</em> beetles. “No-choice” laboratory assays tested the susceptibility of <em>A. biguttatus</em> Fabricus eggs and larvae and <em>A. sulcicollis</em> Lacordaire and <em>A. convexicollis</em> Redtenbacher eggs to the parasitoids. <em>Oobius agrili, T. planipennisi</em> and <em>S. galinae</em> all attacked <em>A. biguttatus,</em> and offspring were produced. <em>Oobius agrili</em> also attacked the eggs of <em>A. convexicollis,</em> but not <em>A. sulcicollis</em>. This study is the first to show parasitism of a non-target species by <em>T. planipennisi.</em> Further work is needed to fully assess the non-target risk of these parasitoids for release using more ecologically relevant tests, such as ‘choice’, semi-field and chemical ecology assays on the attacked <em>Agrilus</em> species.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"200 ","pages":"Article 105678"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143179875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Subsequent chemical and foraging ecology preferences of Theocolax elegans (Westwood) (Hymenoptera: Pteromalidae) reared on two alternate stored product host insects","authors":"Jacqueline M. Maille ,&nbsp;Chloe E. Albin ,&nbsp;Rachel R. Harman ,&nbsp;Matthew C. Hetherington ,&nbsp;Sabita Ranabhat ,&nbsp;Jennifer Abshire ,&nbsp;Jaye Montgomery ,&nbsp;Ian M. Stoll ,&nbsp;Madison Lillich ,&nbsp;Samantha Gillette ,&nbsp;Daniel Brabec ,&nbsp;Kun Yan Zhu ,&nbsp;Erin D. Scully ,&nbsp;Alison R. Gerken ,&nbsp;William R. Morrison III","doi":"10.1016/j.biocontrol.2024.105665","DOIUrl":"10.1016/j.biocontrol.2024.105665","url":null,"abstract":"<div><div><em>Theocolax elegans</em> (Hymenoptera: Pteromalidae) is a potential postharvest biocontrol agent whose host range includes <em>Sitophilus oryzae</em> (Coleoptera: Curculionidae) and <em>Rhyzopertha dominica</em> (Coleoptera: Bostrichidae). Both host species are cosmopolitan and destructive pests of bulk wheat. In addition, either species could be used when mass rearing <em>T. elegans</em>. The Hopkins-Host Selection principle suggests the natal host environment (e.g., the habitat in which a wasp emerges from a pupa) may influence the semiochemicals an organism utilizes when foraging for oviposition sites. Thus, later efficacy may be impaired if important semiochemicals are lost from the foraging repertoire of <em>T. elegans</em>. In order to investigate the impact of natal host environment on the behavioral response of <em>T. elegans</em> to potential hosts, we reared <em>T. elegans</em> on either <em>S. oryzae</em> or <em>R. dominica</em> for multiple generations. We then evaluated the orientation and taxis of<!--> <em>T. elegans</em> <!-->to six treatments: <em>S. oryzae</em>,<!--> <em>R. dominica,</em> damaged grain + <em>S. oryzae</em>, damaged grain + <em>R. dominica</em>, damaged grain + insects from the natal environment, or an undamaged control. We found <em>T. elegans</em> reared on <em>R. dominica</em> most preferred damaged grain from <em>R. dominica</em> in a four-way olfactometer, which was 4.2-fold more often chosen than <em>S. oryzae</em> individuals alone. Treatments containing <em>R. dominica</em> were differentiated from others based on headspace volatiles while <em>S. oryzae</em>-infested grain generally overlapped with uninfested grain. Both rearing host and subsequent foraging host affected efficacy of <em>T. elegans</em> released in a pilot-scale elevator. Wasps appeared most effective in suppressing grain damage by 35–38 % when reared on <em>R. dominica</em> and foraging for <em>R. dominica</em> compared to <em>S. oryzae-</em>reared wasps that only reduced damage by 1–18 %. <em>Sitophilus oryzae</em>-reared wasps only effectively foraged on hosts up to 0.5 m, while <em>R. dominica</em>-reared wasps foraged successfully up to 4 m. Overall, we found that the natal host and chemical cues significantly affected taxis and foraging by <em>T. elegans</em>, suggesting that careful attention should be paid to the mass rearing procedure for this parasitoid.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"200 ","pages":"Article 105665"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143181459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Projected range overlap between the predator Teretrius nigrescens and the invasive stored product pest Prostephanus truncatus expands under climate change","authors":"Rachel R. Harman,&nbsp;William R. Morrison III,&nbsp;Alison R. Gerken","doi":"10.1016/j.biocontrol.2024.105682","DOIUrl":"10.1016/j.biocontrol.2024.105682","url":null,"abstract":"<div><div>The larger grain borer, <em>Prostephanus truncatus</em> (Horn) (Coleoptera: Bostrichidae), is a forest-dwelling destructive pest of stored corn and cassava native to Central America and invasive in Africa. Its native predator, <em>Teretrius nigrescens</em> Lewis (Coleoptera: Histeridae), was released in Africa as a biocontrol agent with some success. In the native range, the predator does control the pest, which has likely limited the pest’s movement northward into the United States. However, responses to future changes in climate may impact the ranges of insect species differently and, consequently, may alter the efficacy of biological control. Thus, we (1) used bioclimatic variables and known occurrences to model the potential distribution of <em>T. nigrescens</em> and <em>P. truncatus</em> in its native and introduced ranges, (2) projected future potential distribution out to the years 2050 and 2070 under low and high climate change scenarios (representative concentration pathways (RCP) 2.6 and 8.5, respectively), and (3) compared the ranges and important bioclimatic variables between the species. We used the platform Wallace, which employs an optimized maximum entropy (MaxEnt) model. Highly suitable areas (&gt;75 %) of <em>T. nigrescens</em> current distribution totaled 5.5 % and 8.2 % for the Americas and Africa, respectively. Areas of high suitability are estimated to double (RCP2.6) and to triple (RCP8.5) by 2050 and then increase again 1.3 times by 2070 under RCP8.5. Projected <em>T. nigrescens</em> distributions are much smaller in area and percentage expansion than prey. The overlap of current highly suitable distributions is lower in the native (4.5 %) than the African range (22.2 %); however, the overlap is expected to increase by 86 % in the Americas and 63 % in Africa under the 2070 RCP8.5. Bioclimatic variables important to the models show that low temperatures and variability in temperature and humidity limit the biocontrol agent’s distribution to a greater degree than the prey.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"200 ","pages":"Article 105682"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143181275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trait diversity and spider community composition are associated with lower herbivory in young forest plantations","authors":"Warbota Khum ,&nbsp;Ondřej Košulič ,&nbsp;Radek Michalko","doi":"10.1016/j.biocontrol.2024.105666","DOIUrl":"10.1016/j.biocontrol.2024.105666","url":null,"abstract":"<div><div>Spiders constitute a numerically dominant group of generalist predators in forest ecosystems, but their biocontrol function in forest plantations is not well understood. The biocontrol potential of spiders may depend upon interaction among pest type, forest type, season, trait composition, and spider community diversity. Using a correlative approach, we addressed this gap in knowledge in young (10–15 years) oak (<em>Quercus</em> spp.) and ash (<em>Fraxinus</em> spp.) forest plantations. We sampled (N = 103 samples) foliage-dwelling arthropods and leaves during late spring and autumn. We then measured traits of spiders (body size and hunting strategy) and examined two indicators of herbivory (leaf damage and leaf dry biomass). In oak plantations, abundances of Hemiptera negatively correlated with functional diversity of spiders consistently during the two seasons. Abundances of caterpillars negatively correlated with abundances of ambushers and marginally with abundances of orb-web building spiders during late spring. Abundances of herbivorous Coleoptera negatively correlated with functional evenness of spiders in autumn. Moreover, herbivory negatively correlated with abundances of Space-web builders and functional diversity of spiders in spring but with mean spider body size in autumn. In ash plantations, herbivory negatively correlated with spider abundances in autumn. Our findings provide indirect evidence that foliage-dwelling spiders can be useful for the biological control of forest pests. The biocontrol potential of spiders seems to depend on both functional diversity and identity. Therefore, forest pest management should focus not only on spider community composition but also functional trait diversity of spiders.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"200 ","pages":"Article 105666"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143181460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predation behaviors of Pterostichus melanarius adults on larvae of the soybean gall midge, Resseliella maxima","authors":"Sarah C. von Gries,&nbsp;Amelia R.I. Lindsey,&nbsp;Robert L. Koch","doi":"10.1016/j.biocontrol.2024.105679","DOIUrl":"10.1016/j.biocontrol.2024.105679","url":null,"abstract":"<div><div>Soybean gall midge, <em>Resseliella maxima</em> Gagné (Diptera: Cecidomyiidae), is a pest of soybean that causes significant yield losses. <em>Pterostichus melanarius</em> (Illiger) (Coleoptera: Carabidae) has been reported as the most abundant predator of <em>R. maxima</em> in the field and feeds on <em>R. maxima</em> in the laboratory. Understanding the feeding behaviors of <em>P. melanarius</em> is crucial to determining the potential of this predator as a biological control agent for <em>R. maxima</em>. We conducted laboratory-based experiments on the functional response and prey preference of <em>P. melanarius</em>. The functional response study was performed with densities of <em>R. maxima</em> ranging from 1 to 60 larvae per Petri dish. <em>Pterostichus melanarius</em> exhibited a Type I (i.e., linear) functional response after 1 hr with an estimated handling time (T_h) and attack rate (a) of 0.0054 ± 0.0055 (SE) and 0.7076 ± 0.1339 (SE), respectively. The prey preference study was performed with three different ratios of <em>R. maxima</em> larvae and soybean aphids, <em>Aphis glycines</em> Matsumura (Hemiptera: Aphididae) offered as prey. Results indicated that <em>P. melanarius</em> consumed greater ratios of <em>R. maxima</em> to <em>A. glycines</em> than were offered. Overall, the results show that <em>P. melanarius</em> is a voracious predator of <em>R. maxima</em> and prefers <em>R. maxima</em> over <em>A. glycines</em>. These feeding behaviors of <em>P. melanarius</em> suggest promise for biological control of <em>R. maxima</em> by this predator.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"200 ","pages":"Article 105679"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143181336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combining two parasitoids (Asecodes hispinarum and Tetrastichus brontispae) for biological control of coconut leaf beetle","authors":"Qikai Zhang ,&nbsp;Bin Jiao ,&nbsp;Hui Lu ,&nbsp;Jihong Tang ,&nbsp;Xu Zhang ,&nbsp;Baoqian Lyu","doi":"10.1016/j.biocontrol.2024.105688","DOIUrl":"10.1016/j.biocontrol.2024.105688","url":null,"abstract":"<div><div>The coconut leaf beetle (CLB), <em>Brontispa longissima</em>, is a major threat to coconut production worldwide, causing widespread and important losses throughout the tropics. Two eulophid parasitoids, larval parasitoid <em>Asecodes hispinarum</em> and pupal parasitoid <em>Tetrastichus brontispae</em>, are the dominant natural enemies of CLB. However, the combined use of these species for biocontrol has not been systematically explored. We analyzed the interactions of <em>A. hispinarum</em> and <em>T. brontispae</em> and evaluated possible deployment strategies by determining the outcomes of releases under realistic agricultural conditions. Our laboratory studies showed that the concurrent release of the two parasitoid species did not reduce either species lifespan or reproductive output, and it significantly enhanced the impact on CLB. A 3:1 ratio of <em>A. hispinarum</em> to <em>T. brontispae</em>, a 10:1 parasitoid-to-pest ratio, and a regimen of five consecutive monthly releases proved to be the most effective strategy in the laboratory. When this combined release approach was used in field trials over a two-year period, there were significant reductions in pest densities and higher parasitism rates than single-species releases. This study highlights the potential of integrating multiple parasitoid species to improve biological control programs.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"200 ","pages":"Article 105688"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143181273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Persistence of the entomopathogenic nematode Steinernema feltiae in a novel capsule formulation","authors":"Jermaine D. Perier ,&nbsp;Shaohui Wu ,&nbsp;Steven P. Arthurs ,&nbsp;Michael D. Toews ,&nbsp;David I. Shapiro-Ilan","doi":"10.1016/j.biocontrol.2024.105684","DOIUrl":"10.1016/j.biocontrol.2024.105684","url":null,"abstract":"<div><div>The post-application persistence of entomopathogenic nematodes (EPNs) depends on environmental conditions, which affect their efficacy as biocontrol agents in the field. This study evaluated the persistence of the EPN <em>Steinernema feltiae</em> in a novel hydrogel/emulsion capsule formulation under laboratory and field conditions. The new formulation was compared to the traditional aqueous application, using the baiting method with <em>Tenebrio molitor</em> larvae. In laboratory experiments, the capsule formulation of <em>S. feltiae</em> ENO2 strain was compared with aqueous applications of ENO2 and SN strain at 23 °C for 0, 7, 14, 21, and 28 days after treatment (DAT) in soil cups. The ENO2 capsule formulation persisted longer than ENO2 aqueous, while SN aqueous also persisted better than the ENO2 aqueous application but was not different from the ENO2 treatment. In field trials, the capsule formulation (applied sub-surface) persisted longer than the aqueous application (applied on the surface) of ENO2 when applied in recommended methods; however, there were no differences between formulations when applied in-furrow (sub-surface) or on the soil surface. Comparing the application methods, the sub-surface application improved the persistence of capsules over the surface application while it did not affect the performance of the aqueous application. Overall, these findings suggest the capsule formulation buried in the soil improves EPN persistence.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"200 ","pages":"Article 105684"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143181277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradation of aflatoxin B1 by a novel mined aldo–keto reductase from Meyerozyma guilliermondii AF01","authors":"Wan Zhang ,&nbsp;Xiaojiao Chang ,&nbsp;Yangying Han ,&nbsp;Tiantian Li ,&nbsp;Jianpeng Dou ,&nbsp;Wen Du ,&nbsp;Wenfu Wu ,&nbsp;Wei Wang ,&nbsp;Zhongjie Zhang ,&nbsp;Changpo Sun","doi":"10.1016/j.biocontrol.2024.105676","DOIUrl":"10.1016/j.biocontrol.2024.105676","url":null,"abstract":"<div><div>Biocontrol is an effective technology for managing mycotoxin contamination in food, and the improvement of its application depends largely on revealing the degradation mechanisms at the molecular level. Research in this area is much less than that on the screening of degrading strains. In a previous study, <em>Meyerozyma guilliermondii</em> AF01 was confirmed to exert degradation and adsorption effects on aflatoxin B₁ (AFB₁). In this study, a potential degradation gene, <em>MG2-4</em>, was mined using a combination of bioinformatics and chemical approaches. The gene was heterologously expressed in <em>Escherichia coli</em> Rosetta DE3, and the recombinant protein, Mg aldo–keto reductase (AKR), reacted with AFB₁ <em>in vitro</em>. Moreover, MgAKR rapidly removed AFB₁. The degradation product was identified as aflatoxicol using ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry, which is the same as the degradation product of the AF01 strain. This study reveals that <em>MG2-4</em> is the key AFB₁-degrading enzyme gene in the AF01 strain and lays the foundation for improving AFB₁ removal using the AF01 strain.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"200 ","pages":"Article 105676"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143181276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}