Biological Control最新文献

{"title":"Characterization of rhizosphere Streptomyces virginiae IRHB6 as a dual-agent for disease suppression and growth promotion in soybean","authors":"Hafsa Ashraf ,&nbsp;Yanru Zhou ,&nbsp;Dengqin Wei ,&nbsp;Yuze Li ,&nbsp;Feng Yang ,&nbsp;Xiaoling Wu ,&nbsp;Weiying Zeng ,&nbsp;Zudong Sun ,&nbsp;Taiwen Yong ,&nbsp;Xiaoli Chang","doi":"10.1016/j.biocontrol.2025.105925","DOIUrl":"10.1016/j.biocontrol.2025.105925","url":null,"abstract":"<div><div><em>Streptomyces</em> spp. are renowned for producing biologically active compounds and serving as biological control agents against plant diseases. In a previous study, <em>Streptomyces</em> strain IRHB6, isolated from the soybean rhizosphere, exhibited significant inhibitory activity against mycelial growth of <em>Fusarium oxysporum,</em> the causative agent of soybean root rot. To further characterize its biocontrol potential, we conducted comprehensive <em>in vitro</em> and <em>in vivo</em> assays to evaluate antagonistic effects on multiple phytopathogens, and assess plant growth-promoting capabilities. Our results showed that strain IRHB6 exhibited a colony morphology characterized by a wrinkled, rough surface texture and was identified as <em>Streptomyces virginiae</em> through multilocus sequence analysis (MLSA). The strain produced hydrolytic enzymes, siderophores, and indole-3-acetic acid (IAA), and exhibited phosphorus-solubilizing activity. A dual-cultural confrontation assay revealed that IRHB6 exhibited antagonistic traits against phytopathogens, particularly, <em>Fusarium</em> species responsible for soybean root rot and <em>Pseudomonas syringae</em> pv. <em>actinidiae</em> for kiwifruit canker. The induced expression of biosynthetic genes for siderophore pyoverdine (<em>pvdA</em>) as well as antibiotic polyketide (<em>T1PKS/NRPS-like</em> and <em>T2PKS</em>) further substantiates its capacity for producing bioactive compounds against phytopathogens. Furthermore, treatment with IRHB6 cell suspension significantly promoted plant growth. Critically, IRHB6 conferred substantial protective capabilities against <em>Fusarium</em> root rot. Based on these findings, <em>S. virginiae</em> IRHB6 represents a promising biocontrol agent for soil-borne disease as well as plant growth promotion.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"211 ","pages":"Article 105925"},"PeriodicalIF":3.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145615030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal volatile organic compounds from Burkholderia sp. BV6 control gray mold disease of cherry tomato","authors":"Yu Zhang ,&nbsp;Minhua Chen ,&nbsp;Jierong Sang ,&nbsp;Shuxin Wei ,&nbsp;Jiming Shen ,&nbsp;Fan Wu ,&nbsp;Shuai Yao ,&nbsp;Yan Huang ,&nbsp;Zhoukun Li ,&nbsp;Zhongli Cui ,&nbsp;Xianfeng Ye","doi":"10.1016/j.biocontrol.2025.105935","DOIUrl":"10.1016/j.biocontrol.2025.105935","url":null,"abstract":"<div><div><em>Botrytis cinerea</em> is a fungal pathogen that acts as a necrotroph, causing gray mold disease and affecting over 1400 plants. The biocontrol effect of microbial volatile organic compounds (VOCs) in controlling postharvest diseases has gradually gained significant attention as a research hotspot. This study evaluated the biocontrol effects of VOCs produced by <em>Burkholderia</em> sp. BV6 on <em>B. cinerea</em> in <em>vitro</em> and in <em>vivo</em>. The results showed that the strain BV6 volatiles suppressed the mycelial growth of <em>B. cinerea</em>. The antifungal effect of strain BV6 volatiles against <em>B. cinerea</em> was enhanced by optimizing the inoculation method and inoculation quantity of strain BV6. The volatiles of strain BV6 showed wide-spectrum antifungal activity against several plant pathogenic fungi. Solid-phase microextraction gas chromatography-mass spectrometry (SPME-GC/MS) analysis identified 24 volatile compounds, the most abundant of which (2-nonanol, 2-nonanone, 2-tridecanol, and 2-undecanol) showed dose-dependent antifungal activity. Among them, 2-nonanol exhibited the strongest antifungal activity toward <em>B. cinerea</em>, with a minimum inhibitory volume (MIV) of 1.5 μL/plate (equal to 21.43 μL/L). Strain BV6 and 2-nonanol reduced the lesion diameter and disease severity of of gray mold in cherry tomatoes caused by <em>B. cinerea</em>. These results suggest that fumigation with 2-nonanol or strain BV6 is a promising method for protecting cherry tomatoes against gray mold disease. Furthermore, this study demonstrated the antifungal efficacy of both strain BV6 and 2-nonanol against <em>B. cinerea</em> and their biocontrol potential for postharvest cherry tomatoes.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"211 ","pages":"Article 105935"},"PeriodicalIF":3.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145680972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lecanicillium enhances kidney bean growth and confers resistance against Frankliniella occidentalis (Thysanoptera: Thripidae) infestation","authors":"Ye-Ming Zhou,&nbsp;Da-Yan Ma,&nbsp;Wen Xie,&nbsp;Ding-yin Li,&nbsp;Jun-Rui Zhi,&nbsp;Xiao Zou","doi":"10.1016/j.biocontrol.2025.105921","DOIUrl":"10.1016/j.biocontrol.2025.105921","url":null,"abstract":"<div><div><em>Frankliniella occidentalis</em> (Pergande) (Thysanoptera: Thripidae), also known as western flower thrips, is a globally distributed insect pest infesting a wide range of crops. We have previously identified <em>Lecanicillium attenuatum</em> and <em>L. cauligalbarum</em> as effective entomopathogenic fungi (EPF) against <em>F. occidentalis</em>. In addition, studies have reported that EPF can colonize plants, promoting plant growth and resisting pests. Therefore, this study aimed to investigate whether these two <em>Lecanicillium</em> species can colonize plants and confer sustained resistance against<!--> <em>F. occidentalis</em>. We found that treating kidney beans with 5 × 10⁷ conidia/mL <em>L. attenuatum</em> resulted in fungal colonization in all plant tissues. Significant enhancements in plant height, fresh weight, taproot biomass, and taproot length were observed after 20 d of treatment with <em>L. attenuatum</em> or <em>L. cauligalbarum.</em> Behavioral tests and developmental bioassays with <em>F. occidentalis</em> were performed to assess the effects of 15-day treatment with 5 × 10⁷ conidia/mL <em>L. attenuatum</em> or <em>L. cauligalbarum</em> on kidney bean plants. Behavioral tests indicated that <em>Lecanicillium</em>-inoculated plants repelled adult <em>F</em>. <em>occidentalis</em>, and developmental bioassays revealed that both <em>L. attenuatum</em> and <em>L. cauligalbarum</em> negatively affected the growth, development, and reproduction of <em>F. occidentalis</em>. Compared with newly eclosed nymphs that fed on control plants, those that fed on <em>L. attenuatum</em>- or <em>L. cauligalbarum</em>-inoculated plants had substantially shorter survival, with only 57.8 % or 53.3 % of the nymphs developing to the adult stage, respectively. Oviposition of female thrips reared on <em>L. attenuatum</em>- or <em>L. cauligalbarum</em>-inoculated plants was significantly lower (16 and 17 eggs/female, respectively) than that of those reared on uninoculated plants (93 eggs/female). These findings indicate that the endophytes <em>L. attenuatum</em> and <em>L. cauligalbarum</em> are potential biological agents for the control and management of <em>F. occidentalis</em>.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"211 ","pages":"Article 105921"},"PeriodicalIF":3.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145615029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating ensemble modeling into pre-emptive classical biological control: Assessing climate suitability and niche overlap of two natural enemies against Anoplophora chinensis (Coleoptera: Cerambycidae) in China","authors":"Liang Zhang,&nbsp;Chaokun Yang,&nbsp;Ping Wang,&nbsp;Guanglin Xie,&nbsp;Wenkai Wang","doi":"10.1016/j.biocontrol.2025.105932","DOIUrl":"10.1016/j.biocontrol.2025.105932","url":null,"abstract":"<div><div><em>Anoplophora chinensis</em> (Coleoptera: Cerambycidae) is an economically important wood-boring pest in China, posing serious threats to forest ecosystems, with its potential range expansion further intensified by climate change. To address the ecological and economic threats posed by <em>A. chinensis</em>, this study combined ecological niche modeling and species distribution modeling (SDM) to evaluate the biocontrol potential of two key natural enemies, <em>Dastarcus helophoroides</em> (Coleoptera: Bothrideridae) and <em>Sclerodermus sichuanensis</em> (Hymenoptera: Bethylidae). Meanwhile, niche overlap analysis was performed to quantify the ecological niche similarity between these natural enemies and the target pest. The results demonstrate that under the current climate, eastern, northern, and central China are key regions where the suitable habitats of both natural enemies (<em>D. helophoroides and S. sichuanensis</em>) exhibit significant spatial overlap with the distribution range of <em>A. chinensis</em>. However, climate change indicates a potential contraction in the suitable ranges of both natural enemies, in contrast to an expansion in the distribution of <em>A. chinensis</em>. Further analysis identified that bioclimate, vegetation indices, topography, solar radiation, and human activities are the critical environmental factors jointly driving the distribution patterns of <em>A. chinensis</em> and its natural enemies. Additionally, niche overlap analysis indicated that the ecological niches of both natural enemies are highly similar to that of <em>A. chinensis</em>. This study provides new insights into optimizing natural enemy release areas and enhancing classical biological control efficiency, while providing scientific guidance for integrated forest pest management and ecosystem conservation.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"211 ","pages":"Article 105932"},"PeriodicalIF":3.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocontrol potential of root rot in Polygonatum sibiricum by Bacillus velezensis Y24 and its genome analysis","authors":"J.Y. Li ,&nbsp;J. Zhang ,&nbsp;Z.B. Cai ,&nbsp;X.Y. Li ,&nbsp;T. Lou ,&nbsp;X. Zhou ,&nbsp;F.Y. Shen ,&nbsp;L.S. Jiang ,&nbsp;W. Hu ,&nbsp;X. Chen ,&nbsp;L. Sun","doi":"10.1016/j.biocontrol.2025.105924","DOIUrl":"10.1016/j.biocontrol.2025.105924","url":null,"abstract":"<div><div><em>Polygonatum sibiricum</em> root rot, caused by <em>Fusarium oxysporum</em>, is a severe soil-borne disease in <em>P. sibiricum</em> cultivation. In this study, a high-efficiency biocontrol bacterium, <em>Bacillus velezensis</em> Y24, was isolated from healthy <em>P. sibiricum</em> plants, and its biocontrol potential was evaluated. Strain Y24 exhibited an in vitro inhibition rate of 70.63 % against <em>F. oxysporum</em>, while its cell-free supernatant (CFS) showed an inhibition rate of 50.90 %. The EC₅₀ of lipopeptides (LPs) against <em>F. oxysporum</em> was 0.134 mg/mL. After treatment with 0.2 mg/mL LPs, the hyphae of <em>F. oxysporum</em> showed swelling, deformation, and fragmentation, with conidial germination significantly inhibited. The colonization ability assessment indicated that strain Y24 exhibits a strong biofilm-forming capacity, with the most prominent biofilm-forming ability in MSGG medium. Analysis of its whole-genome sequencing using antiSMASH revealed that strain Y24 contains 13 biosynthetic gene clusters (BGCs) for antimicrobial peptide synthesis, among which 9 BGCs show high similarity to known functional gene clusters, including Bacillaene, Fengycin, and Difficidin. Moreover, the genome of strain Y24 also has the potential to synthesize various extracellular proteases and cellulases. In pot experiments, the control efficacy of strain Y24 against <em>P. sibiricum</em> root rot reached 81.51 %. This study its key antifungal substances, laying a solid research foundation for green control of <em>P. sibiricum</em> root rot.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"211 ","pages":"Article 105924"},"PeriodicalIF":3.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145615095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of a new Streptomyces species QH1-20 against pepper anthracnose, and integrated transcriptomic and metabolomic analyses reveal its biocontrol mechanisms","authors":"Zhengnan Li,&nbsp;Lei Zhang,&nbsp;Yisen Wu,&nbsp;Bowen Fan,&nbsp;Pingping Sun","doi":"10.1016/j.biocontrol.2025.105931","DOIUrl":"10.1016/j.biocontrol.2025.105931","url":null,"abstract":"<div><div>Pepper anthracnose is one of the most destructive disease in both the pre- and postharvest of pepper. It is traditionally managed by chemical fungicides, the isolation, and application of the environmental- and human health friendly biological agents for controlling this disease remains underexplored. Utilizing genome sequencing and comparative phylogenetic analysis, a novel <em>Streptomyces</em> species, named as <em>Streptomyces</em> sp. QH1-20, was identified in this work. QH1-20 demonstrated potent antagonistic activity against pepper anthracnose (achieving 98% control efficacy <em>in vivo</em>), and exhibited a broad spectrum of antagonism against other 10 test plant pathogens. QH1-20 enhanced the activity of defense-related enzymes in pepper, and significantly upregulated the expression of peroxisome-associated genes. Integrated transcriptomic and metabolomic revealed that QH1-20 upregulated genes involved in multiple pathways, including tryptophan synthase, phenylpropanoid biosynthesis, biotin metabolism, flavonoid, flavone and flavonol biosynthesis, sesquiterpenoid and terpenoid pathways. Additionally, it modulated the production of compounds such as kievitone, tryptophan, zerumbone, momilactone A and other terpenoid or diterpenoid compounds. Conjoint omics analyses further demonstrated that QH1-20 induced resistance in pepper fruit against anthracnose by converting the linoleic acid to arachidonic acid, which potentially initiate plant defense mechanisms or inhibit reactive oxygen species (ROS) production. These findings provide a comprehensive understanding of the molecular mechanisms by which QH1-20 inhibits postharvest anthracnose in peppers.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"211 ","pages":"Article 105931"},"PeriodicalIF":3.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145680970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome mining and metabolite profiling uncover the biocontrol and plant growth-promoting capacities of Bacillus velezensis TCS001","authors":"Hong-Zhen Jiang ,&nbsp;Lu-Yao Liu ,&nbsp;Ran-Di Yang,&nbsp;Jie Chen,&nbsp;Jing Jin","doi":"10.1016/j.biocontrol.2025.105928","DOIUrl":"10.1016/j.biocontrol.2025.105928","url":null,"abstract":"<div><div><em>Bacillus velezensis</em> TCS001, isolated from Bohai Sea sediments, is a beneficial microbial strain exhibiting both plant growth-promoting properties and antagonistic activity against plant pathogens, with particularly efficacy against <em>Botrytis cinerea</em>. However, the whole genome of TCS001 remains unclear, thus restricting its potential applications. To address this gap, the whole genome of TCS001 has been sequenced and annotated to elucidate the molecular mechanisms underlying its plant growth promoting and antimicrobial activities. The genome analysis revealed that TCS001 comprises a single circular chromosome of 4,124,134 bp, possessing a GC content of 46.18 %. Moreover, 4243 protein-coding genes were predicted. Through genome mining, 13 BGCs encoding secondary metabolites with predicted roles were identified, including members of the surfactin, fengycin, and macrolactin families. Utilizing UHPLC–MS/MS technologies, a total of<!--> <!-->2,277 metabolites in positive ion mode<!--> <!-->and<!--> <!-->1,462 metabolites in negative ion mode<!--> <!-->were identified across the CFS and CLE of TCS001, encompassing compounds such as bergenin, gentiopicroside, mycophenolic acid, indole-3-butyric acid, gibberellin A89, A39, A125, arbutin, esculetin, gallic acid, protocatechuic acid, ponicidin, bruceine D,and other metabolites with antimicrobial, growth promoting, antioxidant, and antitumor properties. Three iturin analogues, namely iturin A2, A3, A4, were purified from the CLE. At 50 mg/L, iturin A2, A3, and A4 all showed above 70 % inhibition of <em>B</em>. <em>cinerea</em> mycelial growth, with iturin A4 (77.48 %) being more effective than A2 (72.82 %) and A3 (74.43 %). Transcriptomic analysis revealed that iturin A4 modulated the expression of genes involved in secondary metabolite biosynthesis, amino acid metabolism, and ABC transporters. These findings further elucidated the genomic basis for its ability to promote plant growth and inhibit pathogen activity.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"211 ","pages":"Article 105928"},"PeriodicalIF":3.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145615100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenology and spatial modeling of native and exotic parasitoids for biological control of fall armyworm (Spodoptera frugiperda) in Africa under changing climate conditions","authors":"Marian Adan ,&nbsp;Henri E.Z. Tonnang ,&nbsp;Klaus Greve ,&nbsp;Christian Borgemeister ,&nbsp;Georg Goergen","doi":"10.1016/j.biocontrol.2025.105929","DOIUrl":"10.1016/j.biocontrol.2025.105929","url":null,"abstract":"<div><div>The fall armyworm (<em>Spodoptera frugiperda</em>) poses a significant threat to African agriculture, particularly maize crops, necessitating sustainable pest management solutions. This study evaluates the phenology and effectiveness of both native (<em>Chelonus bifoveolatus</em>, <em>Cotesia icipe</em>, and <em>Charops</em> sp.) and exotic (<em>Chelonus insularis and Cotesia marginiventris</em> parasitoids species as biological control agents under various temperature regimes and future climate scenarios. Utilizing the Insect Life Cycle Modeling (ILCYM) software, the developmental stages, mortality rates, fecundity, life table parameters, and spatial dynamics of these parasitoids were analyzed across a temperature range of 20 °C to 32 °C. The results reveal that optimal development and minimal mortality for these parasitoids generally occur between 25 °C and 30 °C. Native species like <em>Cotesia icipe</em> and <em>Chelonus bifoveolatus</em> demonstrated robust adaptability and higher reproductive rates under these conditions. In contrast, <em>Chelonus insularis</em>, an exotic species, exhibited a narrower temperature tolerance but showed potential for increased reproductive success under future climate scenarios, particularly in southern and eastern Africa. Spatial modeling highlighted that <em>Cotesia icipe</em> exhibited high oviposition rates in East Africa, while <em>C. insularis</em> showed similar potential across central and southern Africa, making these regions prime candidates for targeted biological control interventions. Conversely, regions in southern Africa displayed higher senescence rates for certain parasitoids, such as <em>Cotesia icipe</em>, suggesting potential limitations in their long-term effectiveness in these areas. Climate change projections under Representative Concentration Pathway (RCP) scenarios 2.6 and 8.5 predict significant shifts in the distribution, activity, and establishment risk of both native and exotic parasitoids by 2050 and 2070. Native species like <em>Cotesia icipe</em> and <em>Charops</em> sp. are expected to maintain or expand their effectiveness across regions, while <em>C. insularis</em> may become increasingly important in new areas as climatic conditions evolve.</div><div>This research provides critical insights into the phenology and spatial ecology of parasitoid species, emphasizing their role in integrated pest management necessary under climate change. The findings underscore the importance of selecting climate-resilient parasitoids for sustainable biological control programs across diverse African agro-ecological zones.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"211 ","pages":"Article 105929"},"PeriodicalIF":3.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145680969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of horizontal transmission of Cordyceps javanica in managing Drosophila suzukii populations","authors":"Ricardo Alberto Toledo-Hernández ,&nbsp;Rodrigo Lasa ,&nbsp;Pablo Montoya ,&nbsp;Pablo Liedo ,&nbsp;Mónica Pulido ,&nbsp;Jorge Toledo","doi":"10.1016/j.biocontrol.2025.105927","DOIUrl":"10.1016/j.biocontrol.2025.105927","url":null,"abstract":"<div><div><em>Drosophila suzukii</em> (Matsumura) is a globally distributed pest of soft fruit crops with significant economic impact. This study evaluated the horizontal transmission of conidia of the entomopathogen, <em>Cordyceps javanica,</em> among <em>D. suzukii</em> adults under laboratory and semi-field conditions. Adults of both sexes acquired 1.5–1.8 × 10⁸ conidia per adult when self-inoculated using a felt tube device. No significant differences in the mean number of matings per cage or the duration of mating were observed between non-inoculated adults (Control) or adults inoculated with viable conidia. A mean 1.2–6.0 × 10⁶ conidia per adult were successfully transferred during the first mating event. Inoculated adults exhibited high mortality rates (87–93 %), which exceeded those observed in conspecifics that acquired conidia during the first mating (57–75 %). Conidia transfer and fungal-induced mortality decreased significantly after the second mating event of males. Infected females in the first mating experienced a moderate reduction in fecundity (53 to 93 eggs/female) compared to non-infected females (130 eggs/female), but fertility remained unaffected (&gt;96 % egg hatch rate). Non-inoculated males that were exposed to inoculated males, can acquire conidia due the male-male interactions, but those males exhibited minimal transmission of conidia to females during their first mating, resulting in negligible fungal-induced mortality to females (&lt;2%). Similar mating rates between inoculated and non-inoculated males were observed in semi-field experiments, but low fungal-induced mortality was recorded under these conditions. Our findings reveal some limitations that can compromise the effectiveness of the use of this entomopathogen with a “lure and infect” approach to control <em>D. suzukii</em>.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"211 ","pages":"Article 105927"},"PeriodicalIF":3.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145615099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling Nicotiana tabacum L. growth promotion and Fusarium root rot biocontrol by endophytic Bacillus spp. from different hosts","authors":"Zerui Feng ,&nbsp;Ruidong Fan ,&nbsp;Yunsheng Wang ,&nbsp;Jing Jing ,&nbsp;Hao Ouyang ,&nbsp;Shaobo Wang ,&nbsp;Tomislav Cernava ,&nbsp;Xiaoyulong Chen","doi":"10.1016/j.biocontrol.2025.105930","DOIUrl":"10.1016/j.biocontrol.2025.105930","url":null,"abstract":"<div><div>Members of the genus <em>Fusarium</em> are considered to be highly prevalent plant pathogens globally. Utilizing biological control methods is an eco-friendly and cost-effective strategy to manage and mitigate the spread of plant diseases. In this study, 326 bacterial strains were isolated from 20 different hosts, and four selected isolates with high antagonistic activity against <em>F. oxysporum</em> B-9-1 were subsequently assessed in detail. Molecular identification revealed that these strains belong to <em>Bacillus</em> spp. Physiological assays indicated that all strains can produce indole-3-acetic acid (IAA), phosphate solubilization, protease, amylase, and cellulase. However, only two strains, <em>B. subtilis</em> A2M2177 and <em>B. subtilis</em> A2M2295, formed hydrolytic halos attributable to siderophore production. Plant growth promotion experiments showed that <em>Nicotiana tabacum</em> L. seedlings inoculated with <em>B. subtilis</em> A2M2177 and <em>B. subtilis</em> A2M2295 exhibited significantly better growth parameters, including plant height, leaf area, stem width, fresh weight, dry weight, and chlorophyll content. In addition, <em>B. subtilis</em> A2M2177 and <em>B. subtilis</em> A2M295 significantly reduced the occurrence and severity of tobacco root rot in greenhouse experiments. Overall, the results indicate a high potential for the use of <em>B. subtilis</em> A2M2177 and <em>B. subtilis</em> A2M2295 as biocontrol agents and plant growth promoters.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"212 ","pages":"Article 105930"},"PeriodicalIF":3.4,"publicationDate":"2025-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145788956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}