Biological Control最新文献_第2页

Biological control of Bacillus licheniformis PR2 against Fusarium oxysporum and growth promotion of Pinus strobus L. as an urban greening strategy 地衣芽孢杆菌PR2防治尖孢镰刀菌及促进油松生长的城市绿化策略

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biological Control

Pub Date : 2026-01-16 DOI: 10.1016/j.biocontrol.2026.105966

Jae-Hyun Moon, Henry B. Ajuna, Su-In Choi, Ju-Yeol Yun, Su-Yeon Lee, Vantha Choub, Young Sang Ahn

Eastern white pine (Pinus strobus L.) is an evergreen conifer with a beautiful rounded pyramidal canopy, growing up to 80 feet tall and 40 feet wide, with a lifespan of approximately 200 years, making it an excellent tree for urban greenery, shade, windbreak, living fence/hedge, as well as for crafts and outdoor Chrismas decorations worldwide. However, it is highly susceptible to notorious fungal pathogens such as Fusarium oxysporum at various of growth, from nursery seedlings to mature trees in the field, causing lasting effects such as growth retardation or death. Therefore, this study aimed to evaluate the potential of Bacillus licheniformis PR2 as an alternative to chemical fungicides and fertilizers in the management of Fusarium infections and the promotion of growth of P. strobus seedlings in the nursery. B. licheniformis PR2 produced fungal cell wall-degrading enzymes such as chitinase, β-1,3-glucanase and protease, which degraded chitin, β-glucan and glycoprotein polymers that constitute the cell wall of F. oxysporum. Consequently, the bacterial treatment inhibited spore germination and mycelial growth of F. oxysporum by 85.9% and 61.5%, respectively, at 100% concentration of the crude enzyme fraction. The nursery experiment, treatment of P. strobus seedlings with B. licheniformis PR2 culture increased the survival rate of seedlings by 2.3- and 2.1-fold compared to chemical fertilizer treatment and the control groups, respectively. In addition, B. licheniformis PR2 produced up to 108.9 mg/mL of indole-3-acetic acid (IAA), a plant hormone that promotes root growth and lateral root formation, thereby promoting the uptake of water and nutrients by seedlings. Consequently, treatment of P. strobus seedlings with the bacterial culture of B. licheniformis PR2 improved the root collar diameter, dry weight of shoots and roots compared to the chemical fertilizer treatment and control group, respectively. Therefore, this study demonstrates that B. licheniformis PR2 could be applied as an eco-friendly alternative to chemical fungicides and fertilizers in the sustainable development of urban green spaces, since it can effectively suppress fungal pathogens and promote seedling growth without leaving harmful environmental residues or posing a healthy risk to urban residents.

东方白松（Pinus strobus L.）是一种常绿针叶树，有一个美丽的圆形金字塔状树冠，长到80英尺高，40英尺宽，寿命约为200年，使其成为城市绿化，遮荫，防风林，生活围栏/树篱，以及世界各地的工艺品和户外圣诞装饰的绝佳树木。然而，它是高度敏感的臭名昭著的真菌病原体，如尖孢镰刀菌在不同的生长，从苗圃幼苗到成熟的树木在田间，造成持久的影响，如生长迟缓或死亡。因此，本研究旨在评价地衣芽孢杆菌PR2作为化学杀菌剂和化肥的替代品，在苗圃中防治镰刀菌感染和促进油菜幼苗生长方面的潜力。B. licheniformis PR2产生几丁质酶、β-1,3-葡聚糖酶和蛋白酶等真菌细胞壁降解酶，降解构成尖孢霉细胞壁的几丁质、β-葡聚糖和糖蛋白聚合物。结果表明，当粗酶组分浓度为100%时，细菌处理对尖孢镰刀菌孢子萌发和菌丝生长的抑制作用分别为85.9%和61.5%。苗圃试验中，地衣芽孢杆菌PR2培养物处理水杨树幼苗的成活率分别比化肥处理和对照组提高2.3倍和2.1倍。此外，B. licheniformis PR2产生高达108.9 mg/mL的吲哚-3-乙酸（IAA），这是一种促进根生长和侧根形成的植物激素，从而促进幼苗对水和养分的吸收。由此可见，与化肥处理和对照组相比，地衣芽孢杆菌PR2细菌培养处理的水杨树幼苗的根颈直径、茎干质量和根系干质量均有所提高。因此，本研究表明，地衣芽孢杆菌PR2可以作为化学杀菌剂和肥料的环保替代品，在城市绿地的可持续发展中应用，因为它可以有效地抑制真菌病原体，促进幼苗生长，而不会留下有害的环境残留物，也不会对城市居民造成健康风险。

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引用次数: 0

Development and validation of a screening method for actinomycetes from Protaetia brevitarsis larval frass with biocontrol potential 具有生物防治潜力的白弧菌幼虫放线菌筛选方法的建立与验证

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biological Control

Pub Date : 2026-01-14 DOI: 10.1016/j.biocontrol.2026.105967

Qi Peng , Lida Zhang , Chang Qi , Lili Geng , Hanxiang Wu , Wensheng Xiang , Jie Zhang , Xiangjing Wang , Changlong Shu

This study aimed to establish an efficient screening method for identifying biocontrol strains effective against soil-borne pathogens of plants. To achieve this objective, we developed a quantitatively-controlled potted plant testing (QC-PPT) system by optimizing cultivation devices, growth substrates, pathogen inoculation methods, and quantitative evaluation of root infection. This design effectively confines the roots and facilitates uniform pathogen infection. The optimal inoculation timing was determined to be between the 10th and 16th day of growth. Furthermore, a substrate composed of vermiculite with 4% organic compost was identified as ideal, supporting vigorous peanut growth while allowing sufficient pathogen infection required for reliable biocontrol evaluation. Using this system, seven strains with strong antagonistic effects against Sclerotium rolfsii were isolated from White-spotted Flower Chafer (WSFC, Protaetia brevitarsis) larval frass. In vitro assays showed that strain X13 inhibited Sclerotium rolfsii growth by 44.56%, while strain X15 performed more excellently in the QC-PPT system: it increased peanut root dry weight by 32.8% and reduced root lesion area by 51.2% compared to the control group. Genomic sequencing data revealed that the superior strain X15 possesses the most diverse set of secondary metabolite biosynthetic gene clusters. Collectively, strain X15 is a promising candidate for biopesticide development, and the QC-PPT system we established can be extended to other crop-soil-borne pathogen systems. This study not only provides an effective biocontrol resource for peanut southern blight but also facilitates the development of sustainable disease management strategies in agriculture.

本研究旨在建立一种有效筛选植物土传病原菌生物防治菌株的方法。为了实现这一目标，我们通过优化培养设备、生长基质、病原菌接种方法和根系侵染的定量评价，开发了定量控制盆栽植物检测（QC-PPT）系统。这种设计有效地限制了根系，促进了病原体的均匀感染。确定最佳接种时间为生长第10 ~ 16天。此外，由蛭石和4%有机堆肥组成的基质被认为是理想的，既能支持花生的强劲生长，又能让足够的病原体感染，从而进行可靠的生物防治评价。利用该系统从白斑花（WSFC, Protaetia brevitarsis）幼虫中分离到7株对罗氏菌核菌具有较强拮抗作用的菌株。体外实验表明，菌株X13对罗氏菌核菌生长的抑制作用为44.56%，而菌株X15在QC-PPT体系中的表现更为优异，与对照组相比，菌株X13使花生根系干重增加32.8%，根系损伤面积减少51.2%。基因组测序数据显示，优势菌株X15具有最多样化的次生代谢物生物合成基因簇。综上所述，菌株X15是一个很有前景的生物农药开发候选菌株，我们建立的QC-PPT系统可以推广到其他作物-土壤传播的病原体系统。本研究不仅为花生南枯病提供了有效的生物防治资源，而且为制定可持续的农业病害管理策略提供了依据。

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引用次数: 0

Pleiotropic regulatory function of two component system CbrAB in rhizosphere bacterium Pseudomonas protegens FD6 双组分系统CbrAB对根际假单胞菌蛋白FD6的多效调控作用

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biological Control

Pub Date : 2026-01-10 DOI: 10.1016/j.biocontrol.2026.105958

Jieyu Hu, Yinyin Liu, Cheng Wang, Tao Wu, Qingxia Zhang

Pseudomonas protegens FD6 is an effective biocontrol agent that confers protection against tomato bacterial wilt disease caused by Ralstonia solanacearum. In a two-component system, CbrAB regulated different physiological functions to adapt to diverse environments in pseudomonads and Azotobacter spp.. Here, we explored the global regulatory function of CbrAB on biocontrol-associated traits in P. protegens FD6. CbrAB is involved in several functions, such as antibiotic production, motility, biofilm formation and stress response. Mutation in CbrAB system leads to a loss of the ability to control tomato bacterial wilt and growth defects in LB broth during the logarithmic phase. Additionally, CbrAB might regulate 2,4-diacetylphloroglucinol (2,4-DAPG) and pyoluteorin (PLT) production. The luminescence of luciferase reporter system data suggested that CbrA positively modulates the transcription of the antibiotic synthesis genes pltL and phlA. CbrB differentially regulates the expression of pltL and phlA at the transcriptional level, which is consistent with the results of the quantitative analysis of antibiotics. In this study, we also show that cbrA and cbrB contribute to the efficient colonization of tomato roots by P. protegens FD6. Compared with the wild type, both cbrA and cbrB mutants were also less competitive on tomato roots. We further revealed cbrB positively regulated transcription of algD and flgB, responsible for alginate synthesis and swimming motility, respectively, and sRNAs crcY and crcZ by directly binding to their upstream sequences. Overall, CbrAB in P. protegens contributes to its biological control activity, persistence during root colonization and adaptation to complicated environments.

假单胞菌蛋白FD6是防治番茄青枯病的有效生物防治剂。在双组分系统中，CbrAB调节假单胞菌和固氮细菌的不同生理功能以适应不同的环境。在此，我们探索了CbrAB对P. protegens FD6生物防治相关性状的全球调控功能。CbrAB参与多种功能，如抗生素的产生、运动、生物膜的形成和应激反应。在对数期，brab系统突变导致对番茄青枯病和生长缺陷的控制能力丧失。此外，CbrAB可能调节2,4-二乙酰间苯三酚（2,4- dapg）和pyoluteorin （PLT）的产生。荧光素酶报告系统的发光数据表明，CbrA正调节抗生素合成基因pltL和phlA的转录。CbrB在转录水平上差异调控pltL和phlA的表达，这与抗生素的定量分析结果一致。在这项研究中，我们还发现cbrA和cbrB有助于P. protegens FD6在番茄根部的有效定植。与野生型相比，cbrA和cbrB突变体在番茄根部的竞争能力也较弱。我们进一步发现，cbrB通过直接结合sRNAs crcY和crcZ上游序列，正调控algD和flgB（分别负责海藻酸盐合成和游泳运动）的转录。综上所述，P. protegens中的CbrAB对其生物控制活性、根定殖过程中的持久性以及对复杂环境的适应都有重要作用。

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引用次数: 0

Screening biocontrol agents based on pathogen-induced rhizosphere microbiome modulation and synergistic defense response of cotton 基于病原菌诱导的棉花根际微生物群调节和协同防御反应筛选生物防治剂

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biological Control

Pub Date : 2026-01-05 DOI: 10.1016/j.biocontrol.2026.105959

Xiaopan Du , Chao Zhou , Lulu Wang , Yanyan Dang , Bo Gao , Genlin Zhang

Control of cotton Verticillium wilt still faces significant challenge due to the frequent genetic variation and persistent survival of Verticillium dahliae. Here, we developed a method to reconstruct rhizosphere microbiome based on the “cry for help” hypothesis of pathogen-induced rhizosphere microbiome modulation and to fast isolate antagonistic microorganisms. The drench-inoculated cotton with V. dahliae V592 triggered to enrich soil bacterial species and induced the enrichment of beneficial bacteria in cotton rhizosphere, in which Bacillus amyloliquefaciens E2 with 76.47 % inhibition rate against V. dahliae V592 was obtained. The pot experiment confirmed the ultra-high colonization of B. amyloliquefaciens E2 in cotton root (7.92 × 10⁶ CFU/g) and stems (3.91 × 10⁶ CFU/g). This strain resulted in 66.67 % of control efficacy on cotton Verticillium wilt and reduced the disease index by 45.83 (from 68.75 to 22.92). The application of B. amyloliquefaciens E2 efficiently inhibited colonization of V. dahliae V592 in cotton root, promoted cotton growth, induced ROS burst and callose deposition in cotton leaves, enhanced root vigor (70.72 %), and upregulated the defense-related gene (POD, SOD, PAL, PPO and PR10) expression (3.5-fold to 9.8-fold), thus demonstrating synergistically induced defense responses of cotton plants. The present study provides an effective application of ’cry for help’ concept for fast screening biocontrol agents, and the obtained B. amyloliquefaciens E2 shows the potential for application.

由于大丽花黄萎病的遗传变异频繁和持续存在，棉花黄萎病的防治仍面临重大挑战。本研究基于病原菌诱导根际微生物群调节的“呼救”假说，提出了一种重建根际微生物群并快速分离拮抗微生物的方法。用大丽花弧菌V592对棉花进行水培后，可诱导棉花根际有益菌的富集，其中解淀粉芽孢杆菌E2对大丽花弧菌V592的抑制率为76.47%。盆栽试验证实解淀粉芽孢杆菌E2在棉花根（7.92 × 106 CFU/g）和茎（3.91 × 106 CFU/g）上具有超高定植量。该菌株对棉花黄萎病的防效为66.67%，病指数由68.75降至22.92，降低45.83。解淀粉芽孢杆菌E2有效抑制大丽弧菌V592在棉花根部的定殖，促进棉花生长，诱导棉花叶片ROS爆发和钙质层沉积，增强根系活力（70.72%），上调防御相关基因（POD、SOD、PAL、PPO和PR10）表达（3.5 ~ 9.8倍），显示出协同诱导棉花植株的防御反应。本研究为快速筛选生物防治剂提供了“呼救”概念的有效应用，获得的解淀粉芽孢杆菌E2具有应用潜力。

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引用次数: 0

Management of Bemisia tabaci MEAM1 (Hemiptera: Aleyrodidae) on poinsettia using a papaya banker plant system for the predator Delphastus pallidus (Coleoptera: Coccinellidae) 利用木瓜银行植物系统对一品红上的烟粉虱MEAM1（半翅目：粉虱科）进行治理

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biological Control

Pub Date : 2026-01-01 DOI: 10.1016/j.biocontrol.2025.105952

Young-gyun Park , Erich N. Schoeller , Muhammad Z. Ahmed , Cindy L. McKenzie , Lance S. Osborne

The sweet potato whitefly, Bemisia tabaci Middle East-Asia Minor 1 (MEAM1), is a major global pest of greenhouse crops, causing significant economic losses through feeding damage, honeydew production, and virus transmission. Delphastus pallidus, a specialist predator of whiteflies, has shown potential as an effective biological control agent against B. tabaci. This study evaluated the efficacy of a papaya banker plant system in supporting D. pallidus for B. tabaci MEAM1 control in poinsettias. A three-year greenhouse experiment was conducted with four treatments: a papaya banker plant system with D. pallidus, direct release of D. pallidus without a banker plant, chemical insecticide application, and an untreated control. The densities of whitefly and predator were monitored weekly. Results showed that the papaya banker plant system sustained D. pallidus populations more effectively than a direct release and increased the duration of whitefly suppression. In some instances, the banker plant treatment exhibited greater B. tabaci control efficiency than the insecticide application. These findings suggest that the papaya banker plant system promotes D. pallidus establishment, enhancing biological control of B. tabaci MEAM1. Future studies examining whether this banker plant system can be applied to other crops for controlling B. tabaci as well as other invasive whitefly species and associated crops are necessary to determine the extent of its utility for growers. Additionally, studying the enhancement of predator production in banker plant systems and identifying the optimal ratio of banker plants to target crop plants would likely further improve biological control using the papaya banker plant system.

甘薯粉虱，又称烟粉虱中东小亚细亚1号（Bemisia tabaci Middle - East-Asia Minor 1, MEAM1），是全球温室作物的主要害虫，通过食性损害、产蜜和病毒传播造成重大经济损失。白粉虱是白蝇的专门捕食者，作为一种有效的生物防治剂，白粉虱已显示出潜力。本研究评价了番木瓜银行植株系统对白僵菌防治一品红烟粉虱MEAM1的效果。在为期3年的温室试验中，采用四种处理方法进行了试验，分别是：带苍白菌的番木瓜银行苗系统、不带银行苗直接释放苍白菌、施用化学杀虫剂和未经处理的对照。每周监测白蝇和捕食者密度。结果表明，与直接放生相比，番木瓜银行种植系统能更有效地维持白僵虱种群，并延长了对白僵虱的抑制时间。在某些情况下，植物处理表现出比施用杀虫剂更大的烟粉虱防治效果。这些结果表明，番木瓜银行植株体系促进了白僵菌的建立，增强了对烟粉虱MEAM1的生物防治。未来有必要研究这种银行植物系统是否可以应用于其他作物来控制烟粉虱以及其他入侵白蝇物种和相关作物，以确定其对种植者的效用程度。此外，研究银行植物系统中捕食者产量的提高，确定银行植物与目标作物的最佳比例，可能会进一步提高木瓜银行植物系统的生物防治效果。

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引用次数: 0

Evaluating astigmatid mites as alternative diets for Orius laevigatus: Effects on life history parameters 评估散光螨作为留叶螟的替代饲料：对生活史参数的影响

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biological Control

Pub Date : 2026-01-01 DOI: 10.1016/j.biocontrol.2025.105956

Amador Rodríguez-Gómez , Ana Belén Abelaira , María del Carmen Reche , Virginia Balanza , Pablo Bielza

Integrated pest management (IPM) in greenhouse cultivation has benefited significantly from the use of predators such as Orius laevigatus. This beneficial insect, a natural enemy of thrips, can feed on pollen, facilitating preventive releases in flowering crops. However, in the absence of quality pollen, a supplementary food source is required to facilitate establishment. This study compared the performance of different populations of O. laevigatus, including wild and commercial populations and selected strains adapted to suboptimal pollen diets (1POL and 2POL), all fed with various species of astigmatid mites, a more economical alternative to traditional foods like Ephestia kuehniella eggs and Artemia cysts. The selected strains of O. laevigatus exhibited higher survival during nymphal development and greater fecundity when fed astigmatid mites compared to commercial populations. Among the astigmatid mites, Acarus siro was the best-performing diet, supporting high survival and acceptable development; however, it did not match the fecundity obtained with Ephestia kuehniella eggs. Acarus siro therefore represents a cost-effective supplementary or maintenance food, particularly suitable for sustaining populations in crops and for reducing reliance on Ephestia, especially when using genetically selected O. laevigatus strains that show enhanced performance on suboptimal diets.

温室栽培中有害生物综合治理（IPM）受益于大褐螟（Orius laevigatus）等天敌的使用。这种有益的昆虫是蓟马的天敌，可以以花粉为食，促进开花作物的预防性释放。然而，在缺乏优质花粉的情况下，需要补充食物来源以促进建立。本研究比较了不同种群的laevigatus的表现，包括野生种群和商业种群，以及适应次优花粉饮食（1POL和2POL）的精选菌株，所有菌株都以不同种类的散光螨为食，散光螨是一种比传统食物（如Ephestia kuehniella卵和Artemia cysts）更经济的替代品。与商业种群相比，所选菌株在若虫发育过程中表现出更高的存活率和更大的繁殖力。在散光螨中，小粉螨表现最佳，成活率高，发育良好；但与库氏Ephestia kuehniella卵的繁殖力不匹配。因此，小黄粉蜱是一种具有成本效益的补充或维持食物，特别适用于维持作物种群和减少对艾弗蒂虫的依赖，特别是当使用遗传选择的艾弗蒂虫菌株时，这种菌株在次优饮食中表现出更高的性能。

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引用次数: 0

Using drip irrigation to distribute entomopathogenic nematodes for the control of spotted wing drosophila 滴灌法分布昆虫病原线虫防治斑翅果蝇

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biological Control

Pub Date : 2026-01-01 DOI: 10.1016/j.biocontrol.2025.105951

Isabel B. Lee-Park , Jana C. Lee , Amanda K. Hodson , Fatemeh Ganjisaffar , Dominick D. Skabeikis , Frank G. Zalom

Laboratory studies suggest that entomopathogenic nematodes (EPNs) will successfully infect Drosophila suzukii, spotted-wing drosophila (SWD), a global pest of fruits including cherries, raspberries, blueberries, blackberries, and strawberries. We conducted a series of studies to determine if the EPN, Steinernema carpocapsae, can be delivered through commercial drip irrigation systems that often contain screens, and whether these applications are effective against SWD larvae/pupae. Laboratory studies confirmed that commercially sourced S. carpocapsae can indeed infect SWD pupae and teneral adults and a sieve-shaker study determined that >50 % of EPN infective juveniles (IJs) successfully moved through mesh screen filters with 0.125–0.4 mm openings. In a lathe house semi-field study, EPNs passed through drip tubing with 1 gph button emitters and grower standard drip tape with built-in 0.125 mm mesh filters and successfully infected SWD pupae. Two on-farm field studies confirmed EPNs were able to successfully move through drip tubing and infect SWD and waxworms (positive control) held in mesh sachets under raspberry vines or blueberry bushes. In the raspberry field study, distance from the injection site had little to no effect on EPN distribution. Results indicate that while EPN efficacy is influenced somewhat by irrigation specifications, such as filter mesh size, emitter type and spacing, drip systems with 120-mesh screens or with larger can successfully deliver nematodes resulting in SWD infection in berry cropping systems.

实验室研究表明，昆虫病原线虫（EPNs）将成功感染铃木果蝇、斑翼果蝇（SWD），这是一种全球性的水果害虫，包括樱桃、覆盆子、蓝莓、黑莓和草莓。我们进行了一系列的研究，以确定EPN， steinerma carpocapsae，是否可以通过通常含有筛网的商业滴灌系统输送，以及这些应用是否对SWD幼虫/蛹有效。实验室研究证实，商业来源的S. carpocapsae确实可以感染SWD蛹和一般成虫，一项筛分器研究确定，50%的EPN感染幼虫（IJs）成功通过开口为0.125-0.4毫米的筛网过滤器。在车床房半现场研究中，EPNs通过带有1 gph按钮发射器的滴管和内置0.125 mm网状过滤器的种植者标准滴管，成功感染了SWD蛹。两项农场实地研究证实，epn能够成功地通过滴管传播，感染树莓藤或蓝莓灌木下网眼袋装的SWD和蜡虫（阳性对照）。在覆盆子的田间研究中，距注射部位的距离对EPN的分布几乎没有影响。结果表明，虽然EPN的有效性在一定程度上受到灌溉规格的影响，如过滤器尺寸、发射器类型和间距，但120目或更大的滴灌系统可以成功地输送线虫，导致浆果种植系统中的SWD感染。

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引用次数: 0

Streptomyces yinggelingensis sp. nov. YGL11-2: A novel biocontrol agent with dual activity against banana soil-borne and postharvest fungal diseases YGL11-2：一种新型香蕉土传真菌和采后真菌双重防治剂

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biological Control

Pub Date : 2026-01-01 DOI: 10.1016/j.biocontrol.2025.105947

Chunting Li , Xixi Ou , Dengfeng Qi , Wei Wang , Guopeng Zhu , Yufeng Chen , Miaoyi Zhang , Yankun Zhao , Yongzan Wei , Junting Feng , Kai Li , Dengbo Zhou , Jianghui Xie

Banana production is severely threatened by both Fusarium wilt (caused by Fusarium oxysporum f. sp. cubense tropical race 4, Foc TR4) and anthracnose (caused by Colletotrichum musae), which are devastating soil-borne and postharvest diseases, respectively. Biocontrol using antagonistic microorganisms offers an eco-friendly alternative for managing the concurrent occurrence of soil-borne and postharvest pathogens. In this study, strain YGL11-2 was isolated and characterized as a novel species of Streptomyces through polyphasic taxonomy, for which we propose the name Streptomyces yinggelingensis sp.nov. Genomic analysis of this strain revealed 48 secondary metabolite gene clusters, including compounds such as Ectoine and Anantin C, which exhibit potential antifungal activity. Strain YGL11-2 demonstrated dual antagonistic activity against Foc TR4 and C. musae, with half-maximal effective concentrations (EC₅₀) values of 12.01 mg/L and 25.31 mg/L, respectively. Regarding antifungal mechanisms, strain YGL11-2 extracts disrupted fungal cell membrane structures, caused hyphal and spore wrinkling and breakage, and inhibited spore germination. Pot experiments confirmed that banana seedlings treated with strain YGL11-2 extracts showed a reduced Foc TR4 disease index, achieving a control efficacy of 41.94 %, while significantly promoting root growth (+10.55 cm) and biomass accumulation (+46.40 %). In fruit experiments, bananas treated with strain YGL11-2 exhibited an anthracnose inhibition rate of up to 72.96 %, along with improved quality and delayed ripening. This study is the first to discover a novel Streptomyces species capable of cross-type control of both soil-borne and postharvest diseases, offering a breakthrough solution for controlling both types of banana diseases.

香蕉生产受到枯萎病（由cubense热带小种4号Fusarium oxysporum f. sp. cubense热带小种4，Foc TR4引起）和炭疽病（由炭疽杆菌引起）的严重威胁，这两种疾病分别是破坏性的土传和采后疾病。使用拮抗微生物的生物防治为管理土壤传播和采后病原体的同时发生提供了一种生态友好的选择。本研究分离到菌株YGL11-2，通过多相分类学鉴定为链霉菌新种，并将其命名为Streptomyces yinggelingensis sp.nov。基因组分析显示该菌株有48个次生代谢物基因簇，其中包括具有潜在抗真菌活性的化合物，如Ectoine和Anantin C。菌株YGL11-2对Foc TR4和C. musae具有双重拮抗活性，半最大有效浓度（EC50）分别为12.01 mg/L和25.31 mg/L。在抗真菌机制方面，菌株YGL11-2提取物破坏真菌细胞膜结构，导致菌丝和孢子起皱和断裂，抑制孢子萌发。盆栽试验证实，菌株YGL11-2提取物可降低香蕉幼苗Foc - TR4病害指数，防治效果达41.94%，同时显著促进根系生长（+10.55 cm）和生物量积累（+ 46.40%）。在水果试验中，菌株YGL11-2对香蕉的炭疽病抑制率高达72.96%，并改善了香蕉的品质和延迟成熟。本研究首次发现了一种新型链霉菌，能够跨型控制土传和采后病害，为控制两种香蕉病害提供了突破性的解决方案。

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引用次数: 0

Use of agricultural bioinputs and chemical Inputs: Implications for earwig diversity and pest control in soybean fields 农业生物投入品和化学投入品的使用：对大豆田土蜈蚣多样性和病虫害防治的影响

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biological Control

Pub Date : 2026-01-01 DOI: 10.1016/j.biocontrol.2025.105936

Flávia Fagundes de Paula , Gleice Ane Souza Gonçalves , Nícholas Ferreira Camargo , Gilmar da Silva Nunes , Davi de Lacerda Ramos , André Rangel Nascimento , Eliana Maria Gouveia Fontes

Earwigs are omnivorous predators that feed on a wide range of arthropod pests, including caterpillars, thrips, aphids, and fruit fly larvae, and thus play a critical role in the biological control of pests of economically important crops such as soybeans. The growing adoption of bioinputs as alternatives to synthetic chemical pesticides represents a promising strategy to reduce agrochemical dependence, enhance crop productivity, and support ecosystem-based pest regulation. This study aimed to assess earwig diversity in 19 soybean fields distributed along 11 farms in the state of Goiás, Brazil, subjected to varying levels of bioinput and chemical input application, analyze the population dynamics of earwigs and of their primary prey species, noctuid caterpillars and thrips, and investigate the relationship between the use of bioinputs and chemical inputs and earwig populations. Euborellia annulipes (Lucas) was the most abundant species. A significant negative correlation between earwig abundance and noctuid caterpillar pests and thrips underscores their role as effective biological control agents. Earwig abundance was positively correlated with the use of bioinsecticides and chemical pesticides with lower environmental hazard potential, and negatively affected by highly hazardous insecticides, fungicides, and herbicides. These findings confirm the role of earwigs in integrated pest management and the importance of adopting environmentally conscious agricultural practices that preserve biodiversity and support sustainable crop production.

土蜈蚣是一种杂食性捕食者，以多种节肢动物害虫为食，包括毛虫、蓟马、蚜虫和果蝇幼虫，因此在大豆等重要经济作物的害虫生物防治中起着关键作用。越来越多地采用生物投入品作为合成化学农药的替代品，是减少农用化学品依赖、提高作物生产力和支持基于生态系统的有害生物调控的一种有希望的战略。以巴西Goiás州11个农场19块大豆田为研究对象，对不同生物投入量和化学投入量下地蜈蚣的多样性进行了评价，分析了地蜈蚣及其主要猎物夜蛾毛虫和蓟马的种群动态，探讨了生物投入量和化学投入量与地蜈蚣种群的关系。环带桉叶（Euborellia annulpes, Lucas）是最丰富的种。土蜈蚣丰度与夜蛾害虫和蓟马呈显著负相关，表明其是一种有效的生物防治剂。土蜈蚣丰度与环境危害潜势较低的生物杀虫剂和化学杀虫剂的使用呈正相关，与高危害杀虫剂、杀菌剂和除草剂的使用呈负相关。这些发现证实了蠼螋在病虫害综合治理中的作用，以及采用具有环保意识的农业做法以保护生物多样性和支持可持续作物生产的重要性。

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引用次数: 0

Comparing autodissemination, soil inoculation and their combination as modes of application of Metarhizium anisopliae ICIPE 69 on the melon fly infesting courgette in Kenya under semi-field conditions 比较半田条件下绿僵菌ICIPE 69对肯尼亚西葫芦瓜蝇的自传播、土壤接种及其组合施用方式

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biological Control

Pub Date : 2026-01-01 DOI: 10.1016/j.biocontrol.2025.105955

Rehemah Gwokyalya , Thomas Dubois , Susan K. Onsongo , Joseph A. Odhiambo , Komivi Senyo Akutse , Samira A. Mohamed

The melon fly Zeugodacus cucurbitae (Coquillett) (Diptera: Tephritidae) is a significant pest of cucurbit crops, responsible for substantial yield losses and economic damage across Asia and Sub-Saharan Africa. Control of this pest currently relies heavily on synthetic insecticides, which are associated with environmental and human health risks, and contribute to the development of insecticide resistance. Entomopathogenic fungi offer a promising eco-friendly alternative, yet effectiveness of delivery methods remains a critical limitation in their widespread adoption. This study evaluated the efficacy of three application modes of Metarhizium anisopliae (Mechnikov) Sorokin (Hypocreales: Clavicipitaceae) isolate ICIPE 69, autodissemination devices (AD), soil inoculation (soil), and their combination (AD + soil), for control of Z. cucurbitae in courgette Cucurbita pepo L. (Cucurbitales: Cucurbitaceae) cultivated in field cages. Experiments were conducted in triplicate for two seasons i.e., April to August 2021 (season one) and October 2021 to February 2022 (season two). Infestation with 400 Z. cucurbitae (1: 1 male: female) adults and application of treatments was conducted at the onset of flowering. Fungal conidia acquisition of flies, conidial persistence in soil, Z. cucurbitae population dynamics and C. pepo yield were assessed. Flies in the AD treatment acquired the highest number of conidia (13.17 ± 0.89 conidia/fly) while those in the soil treatment acquired the lowest (0.87 ± 0.27 conidia/fly). AD + soil treatment significantly reduced Z. cucurbitae puparial recovery and suppressed adult fly populations more effectively than the individual treatments. The highest marketable yield was recorded in the AD + soil treated field cages while lowest yield was recorded in the control. These findings underscore the potential of combining entomopathogenic fungus delivery strategies to improve biological control of Z. cucurbitae and enhance crop productivity. Further field evaluations for compatibility with other biological control interventions and economic analyses are recommended to facilitate uptake among farmers.

瓜蝇（双翅目：瓜蝇科）是危害瓜类作物的重要害虫，在亚洲和撒哈拉以南非洲地区造成大量产量损失和经济损失。目前对这种害虫的控制严重依赖于合成杀虫剂，这与环境和人类健康风险有关，并有助于杀虫剂耐药性的发展。昆虫病原真菌提供了一个很有前途的生态友好的替代品，但递送方法的有效性仍然是其广泛采用的关键限制。本研究评价了绿僵菌（Mechnikov） Sorokin （Hypocreales: Clavicipitaceae）分离株ICIPE 69、自传播装置（AD）、土壤接种（土壤）及其组合（AD +土壤）3种施用模式对田间网箱栽培的西葫芦（Cucurbita pepo L.，葫芦科：葫芦科）中黄瓜绿僵菌的防治效果。实验分三次进行，分别为2021年4月至8月（第一季）和2021年10月至2022年2月（第二季）。在开花开始时，用400只葫芦巴成虫（雌雄比例为1:1）侵染和施药。评价了蝇类真菌分生孢子的获取、分生孢子在土壤中的持久性、葫芦小蠊种群动态和葫芦小蠊产量。AD处理的分生孢子数最多（13.17±0.89个/只），土壤处理的分生孢子数最少（0.87±0.27个/只）。AD +土壤处理比单独处理更有效地降低了葫芦田蝇蛹的恢复和抑制成蝇种群。AD +土壤处理的田间笼的可售产量最高，而对照的可售产量最低。这些发现强调了结合昆虫病原真菌递送策略来改善葫芦小蠹的生物防治和提高作物生产力的潜力。建议进一步实地评价与其他生物防治干预措施的兼容性，并进行经济分析，以促进农民吸收。

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引用次数: 0