Biological Control最新文献_第6页

Presence of native and introduced hyperparasitoids raises concerns for the biocontrol of the planthopper Metcalfa pruinosa in Europe 在欧洲，原生和引进的超寄生物的存在引起了人们对飞虱的生物防治的关注

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biological Control

Pub Date : 2025-11-04 DOI: 10.1016/j.biocontrol.2025.105914

L. Mazzon , M. Falasco , E. Guerrieri , I. Martinez-Sañudo , L. Maretto , A. Pozzebon , F. Colombari

Metcalfa pruinosa (Hemiptera, Flatidae) is a Nearctic planthopper that has become an invasive pest in Europe since the late 1970s. It is highly polyphagous, and causes significant damage through sap-sucking, honeydew excretion, and the developmentof sooty mould. To counter its spread, the Nearctic parasitoid Neodryinus typhlocybae (Hymenoptera, Dryinidae) was introduced as a biological control agent. In this two-year study, the mortality of N. typhlocybae cocoons, was investigated. Analysis of over 4800 cocoons collected from 14 sites in northeastern Italy (the area of the parasitoid’s first introduction in Europe) revealed a high cumulative mortality (>70 %), primarily due to predation and hyperparasitism. Nine hyperparasitoid species were identified, with the native Pachyneuron muscarum and the Nearctic Cheiloneurus swezeyi emerging as dominant species. C. swezeyi, reported for the first time in Europe, is known as the primary antagonist of N. typhlocybae in its country of origin. Our study demonstrated a close synchronization with the life cycle of the primary parasitoid and molecular analyses revealed five haplotypes. Emergence patterns highlighted different life strategies between C. swezeyi and P. muscarum, suggesting potential ecological niche partitioning. The specificity of C. swezeyi raises concerns about the long-term efficacy of N. typhlocybae as a biological control agent of M. pruinosa. These findings underscore the complexity of multitrophic interactions in biological control systems and the need for a continuous monitoring of hyperparasitoid communities to assess their impact on M. pruinosa control. Further research is necessary to determine the extent of C. swezeyi distribution across Europe.

梅花飞虱（半翅目，飞虱科）是一种新北极飞虱，自20世纪70年代末以来已成为欧洲的入侵害虫。它是高度多食性的，通过吸取汁液，排出蜜露和发展煤烟霉菌造成重大损害。为了防止其蔓延，引进了新翅目干蝇科的拟寄生物——斑新蚜蝇（Nearctic nedrinus typhlocybae）。在为期两年的研究中，对斑孢乳杆菌茧的死亡率进行了调查。对意大利东北部（该寄生蜂首次传入欧洲的地区）14个地点收集的4800多个茧的分析显示，其累积死亡率很高（70%），主要是由于捕食和过度寄生。共鉴定出9种超寄生物，优势种为本地的Pachyneuron muscarum和Nearctic Cheiloneurus swezeyi。C. swezeyi在欧洲首次报道，在其原产国被认为是伤寒奈瑟菌的主要拮抗剂。我们的研究表明，这与初级拟寄生物的生命周期密切同步，分子分析显示了五种单倍型。出现模式突出了甜叶蝉和白腹弓形虫不同的生命策略，提示了潜在的生态位划分。瑞典梭菌的特异性引起了人们对伤寒奈瑟菌作为pruinosa菌的生物防治剂的长期有效性的关注。这些发现强调了生物防治系统中多营养相互作用的复杂性，以及持续监测超寄生虫群落以评估其对pruinosa控制的影响的必要性。需要进一步的研究来确定瑞典弧菌在欧洲的分布范围。

{"title":"Presence of native and introduced hyperparasitoids raises concerns for the biocontrol of the planthopper Metcalfa pruinosa in Europe","authors":"L. Mazzon , M. Falasco , E. Guerrieri , I. Martinez-Sañudo , L. Maretto , A. Pozzebon , F. Colombari","doi":"10.1016/j.biocontrol.2025.105914","DOIUrl":"10.1016/j.biocontrol.2025.105914","url":null,"abstract":"<div><div>Metcalfa pruinosa (Hemiptera, Flatidae) is a Nearctic planthopper that has become an invasive pest in Europe since the late 1970s. It is highly polyphagous, and causes significant damage through sap-sucking, honeydew excretion, and the developmentof sooty mould. To counter its spread, the Nearctic parasitoid Neodryinus typhlocybae (Hymenoptera, Dryinidae) was introduced as a biological control agent. In this two-year study, the mortality of N. typhlocybae cocoons, was investigated. Analysis of over 4800 cocoons collected from 14 sites in northeastern Italy (the area of the parasitoid’s first introduction in Europe) revealed a high cumulative mortality (>70 %), primarily due to predation and hyperparasitism. Nine hyperparasitoid species were identified, with the native Pachyneuron muscarum and the Nearctic Cheiloneurus swezeyi emerging as dominant species. C. swezeyi, reported for the first time in Europe, is known as the primary antagonist of N. typhlocybae in its country of origin. Our study demonstrated a close synchronization with the life cycle of the primary parasitoid and molecular analyses revealed five haplotypes. Emergence patterns highlighted different life strategies between C. swezeyi and P. muscarum, suggesting potential ecological niche partitioning. The specificity of C. swezeyi raises concerns about the long-term efficacy of N. typhlocybae as a biological control agent of M. pruinosa. These findings underscore the complexity of multitrophic interactions in biological control systems and the need for a continuous monitoring of hyperparasitoid communities to assess their impact on M. pruinosa control. Further research is necessary to determine the extent of C. swezeyi distribution across Europe.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"211 ","pages":"Article 105914"},"PeriodicalIF":3.4,"publicationDate":"2025-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145465225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A test of relative effects of chemotype and environment on biocontrol of invasive Melaleuca quinquenervia in Florida 佛州入侵型五叶白千层虫化学型与环境对其生物防治相对效应的研究

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biological Control

Pub Date : 2025-11-01 DOI: 10.1016/j.biocontrol.2025.105910

Blake Blaskowski , Melissa Smith , Andrea Carmona-Cortez , Anthony Garcia , Peter Stiling

Invasive Melaleuca quinquenervia consists of two different chemotypes, E-viridiflorol and E-nerolidol that differentiate geographically in Florida: E-nerolidol dominates on the east coast and E-viridiflorol is more common on the west coast. Both chemotypes are susceptible to attack from three introduced herbivorous biological control insects from Australia: the leaf-feeding weevil, Oxyops vitiosa, the sap sucking psyllid, Boreioglycaspis melaleucae, and the stem-galling midge, Lophodiplosis trifida. Boreioglycaspis melaleucae were collected from areas in Queensland, Australia supporting only the E-viridiflorol chemotype whereas O. vitiosa and L. trifida were collected more broadly in Australia, in areas supporting both chemotypes. Here, we use reciprocal transplants of E-nerolidol and E-viridiflorol from Florida’s east and west coasts, and censuses of naturally occurring trees on both coasts, to determine if biological control agents (a) prefer one chemotype over another or (b) prefer one coastal site over another. Results showed that there was no chemotype preference of O. vitiosa or L. trifida, but we observed a significant preference for E-viridiflorol by B. melaleucae. There were also differences in herbivore densities between east and west coasts that were likely caused by different environmental conditions. We suggest both consideration of chemotype preference as well as impact of environment when selecting biological control agents for field releases.

侵袭性千层花由两种不同的化学型组成，E-viridiflorol和E-nerolidol在佛罗里达州的地理上存在差异：E-nerolidol在东海岸占主导地位，而E-viridiflorol在西海岸更常见。这两种化学型都容易受到从澳大利亚引进的三种草食性生物防治昆虫的攻击：食叶象鼻虫，vitiosa，吸液木虱，Boreioglycaspis melaleucae和茎瘿蠓，Lophodiplosis trifida。在澳大利亚昆士兰州地区采集到的千层北风甘油三酯仅支持E-viridiflorol化学型，而O. vitiosa和L. trifida在澳大利亚更广泛地采集到，在支持两种化学型的地区。在这里，我们使用来自佛罗里达州东西海岸的E-nerolidol和E-viridiflorol的相互移植，并对两个海岸的自然生长树木进行普查，以确定生物防治剂是否(a)偏爱一种化学型或(b)偏爱一个沿海地点而不是另一个。结果表明，白僵菌对紫僵菌和三叶草菌没有化学型偏好，但对e -绿双氟醇有明显的偏好。东西海岸的草食动物密度也存在差异，这可能是由不同的环境条件造成的。我们建议在选择田间释放的生物防治剂时，既要考虑化学型偏好，又要考虑环境的影响。

{"title":"A test of relative effects of chemotype and environment on biocontrol of invasive Melaleuca quinquenervia in Florida","authors":"Blake Blaskowski , Melissa Smith , Andrea Carmona-Cortez , Anthony Garcia , Peter Stiling","doi":"10.1016/j.biocontrol.2025.105910","DOIUrl":"10.1016/j.biocontrol.2025.105910","url":null,"abstract":"<div><div>Invasive Melaleuca quinquenervia consists of two different chemotypes, E-viridiflorol and E-nerolidol that differentiate geographically in Florida: E-nerolidol dominates on the east coast and E-viridiflorol is more common on the west coast. Both chemotypes are susceptible to attack from three introduced herbivorous biological control insects from Australia: the leaf-feeding weevil, Oxyops vitiosa, the sap sucking psyllid, Boreioglycaspis melaleucae, and the stem-galling midge, Lophodiplosis trifida. Boreioglycaspis melaleucae were collected from areas in Queensland, Australia supporting only the E-viridiflorol chemotype whereas O. vitiosa and L. trifida were collected more broadly in Australia, in areas supporting both chemotypes. Here, we use reciprocal transplants of E-nerolidol and E-viridiflorol from Florida’s east and west coasts, and censuses of naturally occurring trees on both coasts, to determine if biological control agents (a) prefer one chemotype over another or (b) prefer one coastal site over another. Results showed that there was no chemotype preference of O. vitiosa or L. trifida, but we observed a significant preference for E-viridiflorol by B. melaleucae. There were also differences in herbivore densities between east and west coasts that were likely caused by different environmental conditions. We suggest both consideration of chemotype preference as well as impact of environment when selecting biological control agents for field releases.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"210 ","pages":"Article 105910"},"PeriodicalIF":3.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145473511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rhizosphere microorganisms enhance the antiviral activity of the resistance inducer fTDP against tobacco mosaic virus 根际微生物增强了抗性诱导剂fTDP对烟草花叶病毒的抗病毒活性

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biological Control

Pub Date : 2025-11-01 DOI: 10.1016/j.biocontrol.2025.105909

Yueqi Peng , Wenyu Zhang , Wei Zhao , Huanran Zhang, Jianfeng Wang, Xu Wen, Yuting Qi, Banwu Tu, Liping Wu

Rhizosphere microorganisms can act synergistically with biocontrol agents to inhibit soil-borne plant diseases, however, their role in controlling leaf diseases remains unclear. They may, nonetheless, be key factors affecting the stability of biocontrol efficacy against leaf diseases. The resistance inducer fusion TEER-decreasing protein (fTDP) can activate salicylic acid-mediated systemic acquired resistance (SAR) to induce tobacco resistance to infection by the leaf pathogen tobacco mosaic virus (TMV). Here, using amplicon sequencing and quantitative reverse transcription PCR, we analyzed the effects of the rhizosphere microorganism on the antiviral function of fTDP. Simultaneously, a complex microbial consortium resistant to TMV was isolated and screened from the fTDP-treated group. The results showed that fTDP treatment alters soil physicochemical properties, restores the TMV-disrupted rhizosphere microbial community, and enriches beneficial bacterial genera. Furthermore, rhizosphere microorganisms enhanced the antiviral effect of fTDP. Eight bacterial strains isolated from soils after exogenous fTDP application induced plant resistant on TMV by improving the activity of tobacco defense enzymes and promoting plant growth. Six of these strains were successfully combined to construct a complex consortium, which together with fTDP amplified antiviral performance. In summary, fTDP induces changes in the structure of the rhizosphere microbial community, and these microorganisms participate in its antiviral activity. This study offers new insights into the mechanism by which biopesticides suppress foliar pathogens and also contributes to biosafety assessments.

根际微生物可与生物防治剂协同抑制植物土传病害，但其在防治叶片病害中的作用尚不清楚。然而，它们可能是影响叶片病生物防治效果稳定性的关键因素。抗性诱导剂融合teer降低蛋白（fTDP）可激活水杨酸介导的系统性获得性抗性（SAR），诱导烟草对叶病原菌烟草花叶病毒（TMV）的抗性。本文采用扩增子测序和定量反转录PCR技术，分析了根际微生物对fTDP抗病毒功能的影响。同时，从ftdp处理组中分离和筛选了一个复杂的TMV耐药微生物联合体。结果表明，fTDP处理改变了土壤理化性质，恢复了tmv破坏的根际微生物群落，丰富了有益菌属。此外，根际微生物增强了fTDP的抗病毒作用。从外源施用fTDP的土壤中分离出8株菌株，通过提高烟草防御酶活性和促进植株生长，诱导植株对TMV产生抗性。其中6株与fTDP成功结合构建了一个复杂的联盟，该联盟与fTDP一起增强了抗病毒性能。综上所述，fTDP诱导根际微生物群落结构的变化，这些微生物参与了其抗病毒活性。该研究为研究生物农药抑制叶面病原菌的作用机制提供了新的思路，也为生物安全性评价提供了依据。

{"title":"Rhizosphere microorganisms enhance the antiviral activity of the resistance inducer fTDP against tobacco mosaic virus","authors":"Yueqi Peng , Wenyu Zhang , Wei Zhao , Huanran Zhang, Jianfeng Wang, Xu Wen, Yuting Qi, Banwu Tu, Liping Wu","doi":"10.1016/j.biocontrol.2025.105909","DOIUrl":"10.1016/j.biocontrol.2025.105909","url":null,"abstract":"<div><div>Rhizosphere microorganisms can act synergistically with biocontrol agents to inhibit soil-borne plant diseases, however, their role in controlling leaf diseases remains unclear. They may, nonetheless, be key factors affecting the stability of biocontrol efficacy against leaf diseases. The resistance inducer fusion TEER-decreasing protein (fTDP) can activate salicylic acid-mediated systemic acquired resistance (SAR) to induce tobacco resistance to infection by the leaf pathogen tobacco mosaic virus (TMV). Here, using amplicon sequencing and quantitative reverse transcription PCR, we analyzed the effects of the rhizosphere microorganism on the antiviral function of fTDP. Simultaneously, a complex microbial consortium resistant to TMV was isolated and screened from the fTDP-treated group. The results showed that fTDP treatment alters soil physicochemical properties, restores the TMV-disrupted rhizosphere microbial community, and enriches beneficial bacterial genera. Furthermore, rhizosphere microorganisms enhanced the antiviral effect of fTDP. Eight bacterial strains isolated from soils after exogenous fTDP application induced plant resistant on TMV by improving the activity of tobacco defense enzymes and promoting plant growth. Six of these strains were successfully combined to construct a complex consortium, which together with fTDP amplified antiviral performance. In summary, fTDP induces changes in the structure of the rhizosphere microbial community, and these microorganisms participate in its antiviral activity. This study offers new insights into the mechanism by which biopesticides suppress foliar pathogens and also contributes to biosafety assessments.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"210 ","pages":"Article 105909"},"PeriodicalIF":3.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145473512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Several flowering plant species enhance parasitoid performance without benefiting host pests: Implications for conservation biological control in maize agroecosystems 几种开花植物可以提高寄主害虫的寄生性，但对寄主害虫没有好处：玉米农业生态系统保护生物防治的意义

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biological Control

Pub Date : 2025-11-01 DOI: 10.1016/j.biocontrol.2025.105907

Yuanyuan Miao , Xinyi Zhai , Huihui Chen , Jiayin Xie , Min Jin , Yanhui Lu , Zhongxian Lu , Pingyang Zhu

Suitable nectary plants could supply natural enemies for essential nutrients that improve survival, reproduction, and pest-control efficiency. This study evaluated six candidate nectary plants (Brassica napus, Cosmos sulphureus, Cnidium monnieri, Gypsophila paniculata, Kalanchoe blossfeldiana, and Ocimum basilicum) for their potential biological control of six key parasitic natural enemies (Trichogramma chilonis, Trichogramma dendrolimi, Trichogramma japonicum, Trichogramma ostriniae, Microplitis mediator, and Macrocentrus cingulum) and their impacts on four lepidopteran pest species (Ostrinia furnacalis, Spodoptera exigua, Spodoptera frugiperda, and Helicoverpa armigera) in maize. Results showed that B. napus flowers significantly extended the longevity of T. chilonis, M. cingulum females, and both male and female of T. japonicum, while also enhancing the fecundity of T. dendrolimi and T. japonicum. Cosmos sulphureus prolonged the longevity of T. ostriniae males and both sexes of T. japonicum. Cnidium monnieri significantly increased the longevity of all tested parasitoids, including female T. ostriniae, and boosted the fecundity of T. chilonis, T. dendrolimi, and T. japonicum. Ocimum basilicum enhanced the longevity of all tested parasitoids, regardless of sex, and promoted fecundity in T. chilonis, T. dendrolimi, and T. japonicum. Importantly, C. monnieri had no impact on pest longevity. Ocimum basilicum only increased longevity in male S. exigua, with no other significant effects on pest reproduction or survival. These findings indicate that C. monnieri and O. basilicum are promising nectary plants for integration into maize cropping systems to enhance biological control of insect pest.

适宜的蜜糖植物可以为天敌提供必需的营养物质，提高其生存、繁殖和防治害虫的效率。摘要本研究评价了6种候选蜜源植物（甘蓝型油菜、硫芥、蛇耳草、吉菲兰、褐花楸和罗勒木）对6种主要寄生性天敌（赤眼蜂、树突赤眼蜂、日本赤眼蜂、玉米螟赤眼蜂、中介性小黑眼蜂和环心大赤眼蜂）的生物防治潜力，以及对4种鳞翅目害虫（玉米螟、黑眼蜂、黑眼蜂、黑眼蜂、黑眼蜂、黑眼蜂）的生物防治效果。玉米穗夜蛾和棉铃虫。结果表明，甘蓝花能显著延长黄颡鱼、带带绦虫雌虫和日本绦虫雌雄体的寿命，同时也能提高树突绦虫和日本绦虫的繁殖力。硫硫宇宙素延长了雄性玉米螟和雌雄日本螟的寿命。蛇床子显著提高了所有被试拟寄生虫的寿命，包括雌性ostriniae，并提高了chilonis， stendrolimi和japonicum的繁殖力。basilicum提高了所有被试寄生蜂（不分性别）的寿命，并促进了chilonis、stendrolimi和japonicum的繁殖力。重要的是，白僵菌对害虫寿命没有影响。basilicum只是增加了雄鼠的寿命，对害虫的繁殖和存活没有显著影响。这些结果表明，monnieri和O. basilicum是一种很有前途的蜜源植物，可以整合到玉米种植系统中，以加强害虫的生物防治。

{"title":"Several flowering plant species enhance parasitoid performance without benefiting host pests: Implications for conservation biological control in maize agroecosystems","authors":"Yuanyuan Miao , Xinyi Zhai , Huihui Chen , Jiayin Xie , Min Jin , Yanhui Lu , Zhongxian Lu , Pingyang Zhu","doi":"10.1016/j.biocontrol.2025.105907","DOIUrl":"10.1016/j.biocontrol.2025.105907","url":null,"abstract":"<div><div>Suitable nectary plants could supply natural enemies for essential nutrients that improve survival, reproduction, and pest-control efficiency. This study evaluated six candidate nectary plants (Brassica napus, Cosmos sulphureus, Cnidium monnieri, Gypsophila paniculata, Kalanchoe blossfeldiana, and Ocimum basilicum) for their potential biological control of six key parasitic natural enemies (Trichogramma chilonis, Trichogramma dendrolimi, Trichogramma japonicum, Trichogramma ostriniae, Microplitis mediator, and Macrocentrus cingulum) and their impacts on four lepidopteran pest species (Ostrinia furnacalis, Spodoptera exigua, Spodoptera frugiperda, and Helicoverpa armigera) in maize. Results showed that B. napus flowers significantly extended the longevity of T. chilonis, M. cingulum females, and both male and female of T. japonicum, while also enhancing the fecundity of T. dendrolimi and T. japonicum. Cosmos sulphureus prolonged the longevity of T. ostriniae males and both sexes of T. japonicum. Cnidium monnieri significantly increased the longevity of all tested parasitoids, including female T. ostriniae, and boosted the fecundity of T. chilonis, T. dendrolimi, and T. japonicum. Ocimum basilicum enhanced the longevity of all tested parasitoids, regardless of sex, and promoted fecundity in T. chilonis, T. dendrolimi, and T. japonicum. Importantly, C. monnieri had no impact on pest longevity. Ocimum basilicum only increased longevity in male S. exigua, with no other significant effects on pest reproduction or survival. These findings indicate that C. monnieri and O. basilicum are promising nectary plants for integration into maize cropping systems to enhance biological control of insect pest.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"210 ","pages":"Article 105907"},"PeriodicalIF":3.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145412584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and genome analysis of marine Streptomyces albidoflavus S20 for the biological control of tobacco black shank 海洋褐黄链霉菌S20生物防治烟草黑胫病的鉴定与基因组分析

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biological Control

Pub Date : 2025-10-29 DOI: 10.1016/j.biocontrol.2025.105908

Yayue Wang , Fangfang Jia , Ruifang Song , Chong Chen , Tuanwei Pu , Yueqi Xu , Dong Chang , Xuguang Wang , Jianhua Li , Yikuan Fan

Tobacco black shank, a destructive soil-borne disease caused by Phytophthora nicotianae, poses serious threats to global tobacco production. To address this threat, a marine sponge-derived actinomycete, designated strain S20, was isolated and demonstrated potent antagonistic activity against this pathogen. In plate confrontation assays, strain S20 exhibited 90.3 % inhibition of P. nicotianae, and achieved disease control efficiency of 92.1 % and 90.4 % in pot and field trials, respectively. Morphological and molecular characterization identified the strain as Streptomyces albidoflavus. Whole-genome analysis revealed a circular chromosome of 6.73 Mb with 73.12 % GC content, harboring 5,761 protein-coding genes, along with a 218.7 kb plasmid containing 70.47 % GC and 170 open reading frames. AntiSMASH identified numerous BGCs putatively encoding compounds with antifungal, plant growth-promoting, and stress resilience activities. Functional assays showed that strain S20 produced extracellular chitinase, cellulase, β-1,3-glucanase, and protease, in addition to displaying plant-beneficial traits such as nitrogen fixation, phosphate solubilization, siderophore production, and indole-3-acetic acid biosynthesis. Furthermore, treatment with S20 significantly enhanced tobacco seed germination and root elongation, while also increasing the soluble protein content and α-amylase activity. Collectively, our results establish S. albidoflavus S20 as a promising multifunctional biocontrol agent that controls tobacco black shank via direct antagonism and growth promotion, representing a sustainable and effective biocontrol agent.

烟草黑胫病是由烟草疫霉（Phytophthora nicotianae）引起的一种破坏性土传病害，对全球烟草生产构成严重威胁。为了解决这一威胁，分离出一种海洋海绵来源的放线菌，命名为菌株S20，并显示出对该病原体的有效拮抗活性。在平板对抗试验中，菌株S20对烟草疫病菌的抑制率为90.3%，在盆栽和田间试验中分别达到92.1%和90.4%。形态和分子特征鉴定该菌株为白黄链霉菌。全基因组分析显示，该环状染色体全长6.73 Mb， GC含量为73.12%，包含5761个蛋白质编码基因，质粒全长218.7 kb， GC含量为70.47%，开放阅读框为170个。AntiSMASH鉴定出许多BGCs，推测其编码的化合物具有抗真菌、促进植物生长和抗逆性活性。功能分析表明，菌株S20具有胞外几丁质酶、纤维素酶、β-1,3-葡聚糖酶和蛋白酶的活性，并表现出固氮、增磷、产铁和吲哚-3-乙酸等对植物有益的性状。此外，S20处理显著提高了烟草种子发芽率和根伸长，提高了可溶性蛋白含量和α-淀粉酶活性。综上所述，我们的研究结果表明，S. albidoflavus S20是一种很有前途的多功能生物防治剂，通过直接拮抗和促进生长来防治烟草黑胫病，是一种可持续有效的生物防治剂。

{"title":"Characterization and genome analysis of marine Streptomyces albidoflavus S20 for the biological control of tobacco black shank","authors":"Yayue Wang , Fangfang Jia , Ruifang Song , Chong Chen , Tuanwei Pu , Yueqi Xu , Dong Chang , Xuguang Wang , Jianhua Li , Yikuan Fan","doi":"10.1016/j.biocontrol.2025.105908","DOIUrl":"10.1016/j.biocontrol.2025.105908","url":null,"abstract":"<div><div>Tobacco black shank, a destructive soil-borne disease caused by P. nicotianae, and achieved disease control efficiency of 92.1 % and 90.4 % in pot and field trials, respectively. Morphological and molecular characterization identified the strain as S. albidoflavus <!-->S20 as a promising multifunctional biocontrol agent that controls tobacco black shank via direct antagonism and growth promotion, representing a sustainable and effective biocontrol agent.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"211 ","pages":"Article 105908"},"PeriodicalIF":3.4,"publicationDate":"2025-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145435392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Xenorhabdus and Photorhabdus (Morganellaceae) on volatile composition of “Tempranillo” must and on Saccharomyces cerevisiae during alcoholic fermentation Morganellaceae病原菌Xenorhabdus和光habdus对“Tempranillo”must挥发性成分及酒精发酵过程中酿酒酵母的影响

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biological Control

Pub Date : 2025-10-25 DOI: 10.1016/j.biocontrol.2025.105906

Ignacio Vicente-Díez, Mar Vilanova, Pilar Morales, Ramón González, Raquel Campos-Herrera

Viticulture is a global activity producing over eighty million tonnes of grapes annually, 50 % used in winemaking. Traditional viticultural practices still rely on hazardous pesticides, posing environmental and health risks. Recently, Xenorhabdus and Photorhabdus, symbiotic bacteria of entomopathogenic nematodes, have arisen as eco-friendly solutions for pest and disease management in vineyards. However, whether their application can impact the quality of grapes and their microbiota remains unexplored. To unravel the possible impact, we investigated the effect of (i) their direct application on grapes’ volatile composition and (ii) their combination with Saccharomyces cerevisiae in must fermentation. First, we applied unfiltered ferments (UFs) of Xenorhabdus nematophila and Photorhabdus laumondii subsp. laumondii to grapes of Vitis vinifera var. “Tempranillo”. The volatile composition of the grapes was evaluated using solid-phase and gas chromatography-mass spectrometry (SPE-GC/MS). Second, microfermentations using two S. cerevisiae in reconstituted must media were supplemented (1:10) with the treatments (i) cell-free supernatants (CFS), (ii) UFs, and (iii) free-bacterial from X. nematophila and P. laumondii. No significant alterations in the total free volatile fraction were observed, although specific free volatiles (phenyl ethyl alcohol, hexanal, and (E)-2-hexenal) exhibited significant variation under specific treatments. In the glycosidically bound fraction, phenylacetic acid concentrations increased markedly with X. nematophila UFs. In the fermentations, the treatments had no significant impact on CO₂ production kinetics and only slightly affected some fermentation parameters, underscoring their compatibility with yeast-driven fermentation processes. These findings suggest that Xenorhabdus and Photorhabdus can be integrated into vineyard pest management strategies without adversely affecting winemaking parameters or grape aroma quality.

葡萄种植是一项全球性的活动，每年生产8000多万吨葡萄，其中50%用于酿酒。传统的葡萄栽培方法仍然依赖有害的农药，造成环境和健康风险。最近，昆虫病原线虫的共生细菌Xenorhabdus和光habdus已经成为葡萄园病虫害管理的环保解决方案。然而，它们的应用是否会影响葡萄的质量和它们的微生物群仍未研究。为了揭示可能的影响，我们研究了(i)它们直接施用对葡萄挥发性成分的影响以及（ii）它们与酿酒酵母在must发酵中的结合。首先，我们使用了嗜线虫Xenorhabdus nematophilus和laumondii Photorhabdus subsp未经过滤的发酵（UFs）。葡萄品种“丹魄”的葡萄。采用固相和气相色谱-质谱（SPE-GC/MS）对葡萄的挥发性成分进行分析。其次，在重组菌培养基中使用两种酿酒酵母进行微发酵，以1:10的比例补充(i)无细胞上清（CFS），（ii） UFs，和（iii）嗜线虫X.和劳蒙地P.的无细菌。虽然特定的游离挥发物（苯基乙醇、己醛和(E)-2-己烯醛）在特定处理下表现出显著的变化，但总的游离挥发物部分没有观察到显著的变化。在糖苷结合部分中，苯乙酸浓度随着嗜线虫线虫UFs的增加而显著增加。在发酵过程中，这些处理对CO2生产动力学没有显著影响，仅对一些发酵参数有轻微影响，强调了它们与酵母驱动的发酵过程的兼容性。这些研究结果表明，在不影响酿酒参数或葡萄香气品质的情况下，可以将Xenorhabdus和Photorhabdus整合到葡萄园害虫管理策略中。

{"title":"Effects of Xenorhabdus and Photorhabdus (Morganellaceae) on volatile composition of “Tempranillo” must and on Saccharomyces cerevisiae during alcoholic fermentation","authors":"Ignacio Vicente-Díez, Mar Vilanova, Pilar Morales, Ramón González, Raquel Campos-Herrera","doi":"10.1016/j.biocontrol.2025.105906","DOIUrl":"10.1016/j.biocontrol.2025.105906","url":null,"abstract":"<div><div>Viticulture is a global activity producing over eighty million tonnes of grapes annually, 50 % used in winemaking. Traditional viticultural practices still rely on hazardous pesticides, posing environmental and health risks. Recently, Xenorhabdus and Photorhabdus, symbiotic bacteria of entomopathogenic nematodes, have arisen as eco-friendly solutions for pest and disease management in vineyards. However, whether their application can impact the quality of grapes and their microbiota remains unexplored. To unravel the possible impact, we investigated the effect of (i) their direct application on grapes’ volatile composition and (ii) their combination with Saccharomyces cerevisiae in must fermentation. First, we applied unfiltered ferments (UFs) of Xenorhabdus nematophila and Photorhabdus laumondii subsp. laumondii to grapes of Vitis vinifera var. “Tempranillo”. The volatile composition of the grapes was evaluated using solid-phase and gas chromatography-mass spectrometry (SPE-GC/MS). Second, microfermentations using two S. cerevisiae in reconstituted must media were supplemented (1:10) with the treatments (i) cell-free supernatants (CFS), (ii) UFs, and (iii) free-bacterial from X. nematophila and P. laumondii. No significant alterations in the total free volatile fraction were observed, although specific free volatiles (phenyl ethyl alcohol, hexanal, and (E)-2-hexenal) exhibited significant variation under specific treatments. In the glycosidically bound fraction, phenylacetic acid concentrations increased markedly with X. nematophila UFs. In the fermentations, the treatments had no significant impact on CO2 production kinetics and only slightly affected some fermentation parameters, underscoring their compatibility with yeast-driven fermentation processes. These findings suggest that Xenorhabdus and Photorhabdus can be integrated into vineyard pest management strategies without adversely affecting winemaking parameters or grape aroma quality.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"211 ","pages":"Article 105906"},"PeriodicalIF":3.4,"publicationDate":"2025-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145465229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aphid control using natural enemies: Lessons for fine-tuning banana-aphid management 利用天敌控制蚜虫：香蕉蚜虫管理微调的经验教训

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biological Control

Pub Date : 2025-10-22 DOI: 10.1016/j.biocontrol.2025.105905

Christian Adjalla , Bonaventure A. Omondi , Geofrey Ogwal , Walter Ocimati , Peter Wasswa , Guy Blomme

Aphids are serious crop pests and vectors of plant-pathogenic viruses. Natural enemies (parasitoids, pathogens, and predators) can be effective aphid control agents. Vector control can achieve the sustainable management of vector-borne diseases. However, this strategy is under-explored for Banana Bunchy Top Disease (BBTD). This study conducted a systematic review of available literature on aphid control using natural enemies. It has highlighted key opportunities for exploiting and fine-tuning banana-aphid and BBTD control using natural enemies. Relevant literature was identified using PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analysis) guidelines. The search used Scopus and Web of Science databases, employing specific search terms related to aphids and their natural enemies. Data was extracted from 276 publications. A keyword network analysis highlights aphid-parasitoid interactions, aphid-predator interactions, and agroecological aspects of aphid management using natural enemies as key research themes. The analysis also shows natural enemies to have been successfully deployed using classical, augmentative, and conservation strategies. The paper describes their effects on aphids, and the influence of management practices, plant traits, and plant diversity. It also presents examples of agroecological manipulations, within and around fields, and within the broader landscape affecting natural enemies., The mediating effects of crops and agroecological conditions could be explored for controlling banana aphids.

蚜虫是严重的农作物害虫和植物病原病毒的传播媒介。天敌（拟寄生虫、病原菌和捕食者）是防治蚜虫的有效药剂。病媒控制可以实现对病媒传播疾病的可持续管理。然而，这种策略对香蕉束顶病（BBTD）的研究还不够充分。本研究对利用天敌防治蚜虫的文献进行了系统的综述。它强调了利用天敌开发和微调香蕉蚜虫和BBTD控制的关键机会。使用PRISMA（系统评价和荟萃分析首选报告项目）指南确定相关文献。搜索使用了Scopus和Web of Science数据库，使用了与蚜虫及其天敌相关的特定搜索词。数据摘自276份出版物。关键词网络分析强调了蚜虫与拟寄生虫的相互作用，蚜虫与捕食者的相互作用，以及以天敌为主要研究主题的蚜虫管理的农业生态方面。分析还显示，利用经典策略、增强策略和保护策略成功地部署了天敌。本文描述了它们对蚜虫的影响，以及管理措施、植物性状和植物多样性的影响。它还介绍了在农田内部和周围以及在影响天敌的更广泛的景观范围内进行农业生态操纵的例子。探讨作物和农业生态条件对香蕉蚜虫防治的调节作用。

{"title":"Aphid control using natural enemies: Lessons for fine-tuning banana-aphid management","authors":"Christian Adjalla , Bonaventure A. Omondi , Geofrey Ogwal , Walter Ocimati , Peter Wasswa , Guy Blomme","doi":"10.1016/j.biocontrol.2025.105905","DOIUrl":"10.1016/j.biocontrol.2025.105905","url":null,"abstract":"<div><div>Aphids are serious crop pests and vectors of plant-pathogenic viruses. Natural enemies (parasitoids, pathogens, and predators) can be effective aphid control agents. Vector control can achieve the sustainable management of vector-borne diseases. However, this strategy is under-explored for Banana Bunchy Top Disease (BBTD). This study conducted a systematic review of available literature on aphid control using natural enemies. It has highlighted key opportunities for exploiting and fine-tuning banana-aphid and BBTD control using natural enemies. Relevant literature was identified using PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analysis) guidelines. The search used Scopus and Web of Science databases, employing specific search terms related to aphids and their natural enemies. Data was extracted from 276 publications. A keyword network analysis highlights aphid-parasitoid interactions, aphid-predator interactions, and agroecological aspects of aphid management using natural enemies as key research themes. The analysis also shows natural enemies to have been successfully deployed using classical, augmentative, and conservation strategies. The paper describes their effects on aphids, and the influence of management practices, plant traits, and plant diversity. It also presents examples of agroecological manipulations, within and around fields, and within the broader landscape affecting natural enemies., The mediating effects of crops and agroecological conditions could be explored for controlling banana aphids.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"210 ","pages":"Article 105905"},"PeriodicalIF":3.4,"publicationDate":"2025-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145358136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and biocontrol of Planococcus citri Risso (Hemiptera: Pseudococcidae), a new pest of licorice (Glycyrrhiza uralensis Fisch.) using cuticle-degrading enzymes of Bacillus velezensis CE 100 利用velezensis芽孢杆菌表皮降解酶对甘草新害虫柑橘平球菌（半翅目：拟虫科）的鉴定及防制

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biological Control

Pub Date : 2025-10-20 DOI: 10.1016/j.biocontrol.2025.105904

Ju-Yeol Yun , Jae-Hyun Moon , Henry B. Ajuna , Sang-Jae Won , Choub Vantha , Su-In Choi , Su-Yeon Lee , Iksoo Kim , Tae-Joon Kim , Young Sang Ahn

Licorice is a valuable medicinal and industrial crop, but is highly prone to pest damage, especially to polyphagous sap-sucking mealybugs (Hemiptera) insect pests. This study reports for the first the identification citrus mealybugs as insect pests of tremendous economic importance in licorice production. The insect pests collected were identified as Planococcus citri based on analysis of the morphological features and DNA barcode sequencing of mitochondrial Cytochrome Oxidase subunit I (COI) gene. Due to the hazardous effects of chemical insecticides, this study investigated the entomopathogenic potential Bacillus velezensis CE 100 against P. citri. The bacterium produced cuticle-degrading enzymes: lipase, chitinase and protease in a pattern corresponding with cell growth. The maximum lipase, chitinase and protease activity was 32.7 unit/mL, 96.5 unit/mL and 35.7 unit/mL, respectively, which corresponded to the highest cell growth of 25.6 × 10⁷ colony-forming units, observed after 6 days of incubation. Treatment of P. citri with bacterial culture caused the hydrolysis of the cuticular wax layer, chitin laminae and structural proteins of P. citri, resulting in 80.0 % mortality within 6 h after treatment. The control and PB media treatment did not cause insect mortality. The scanning electron microscopy analysis of the treated insects revealed severe cuticle degradation, with deep cracks extending from the epicuticle to the procuticle, and the erosion of the wax layer and broken setae. This study identified P. citri as an insect pest of economic importance in licorice cultivation, for the first time in the world. It also demonstrates the biopesticides potential of B. velezensis CE 100 against P. citri, through the lipolytic, chitinolytic and proteolytic activities, which could enhance licorice production and quality.

甘草是一种有价值的药用和工业作物，但极易受到害虫的危害，特别是多食吸汁粉蚧（半翅目）害虫。本研究首次报道了柑橘粉蚧在甘草生产中具有重要经济意义的害虫鉴定。根据线粒体细胞色素氧化酶亚基I （Cytochrome Oxidase亚基I， COI）基因的形态特征分析和DNA条形码测序，鉴定为柑橘平球菌（Planococcus citri）。由于化学杀虫剂的危害作用，本研究对柑桔芽孢杆菌（Bacillus velezensis CE 100）进行了昆虫病原学研究。细菌产生角质层降解酶：脂肪酶、几丁质酶和蛋白酶，其模式与细胞生长相对应。脂肪酶、几丁质酶和蛋白酶的最高活性分别为32.7、96.5和35.7单位/mL，孵育6 d后，细胞生长达到25.6 × 107个集落形成单位。用细菌培养法处理柑橘，可导致柑橘表皮蜡层、几丁质层和结构蛋白水解，处理后6 h内死亡率达80.0%。对照和PB培养基处理均未引起昆虫死亡。扫描电镜分析显示，处理过的昆虫表皮严重退化，从表皮外皮延伸到外皮有深裂纹，蜡层被侵蚀，刚毛断裂。本研究在世界上首次将柑橘假单胞虫确定为具有重要经济价值的甘草种植害虫。通过对甘草的脂解、几丁质解和蛋白解活性，证明了该菌株对柑橘柑橘的生物农药潜力，可提高甘草的产量和品质。

{"title":"Identification and biocontrol of Planococcus citri Risso (Hemiptera: Pseudococcidae), a new pest of licorice (Glycyrrhiza uralensis Fisch.) using cuticle-degrading enzymes of Bacillus velezensis CE 100","authors":"Ju-Yeol Yun , Jae-Hyun Moon , Henry B. Ajuna , Sang-Jae Won , Choub Vantha , Su-In Choi , Su-Yeon Lee , Iksoo Kim , Tae-Joon Kim , Young Sang Ahn","doi":"10.1016/j.biocontrol.2025.105904","DOIUrl":"10.1016/j.biocontrol.2025.105904","url":null,"abstract":"<div><div>Licorice is a valuable medicinal and industrial crop, but is highly prone to pest damage, especially to polyphagous sap-sucking mealybugs (Hemiptera) insect pests. This study reports for the first the identification citrus mealybugs as insect pests of tremendous economic importance in licorice production. The insect pests collected were identified as Planococcus citri based on analysis of the morphological features and DNA barcode sequencing of mitochondrial Cytochrome Oxidase subunit I (COI) gene. Due to the hazardous effects of chemical insecticides, this study investigated the entomopathogenic potential Bacillus velezensis CE 100 against P. citri. The bacterium produced cuticle-degrading enzymes: lipase, chitinase and protease in a pattern corresponding with cell growth. The maximum lipase, chitinase and protease activity was 32.7 unit/mL, 96.5 unit/mL and 35.7 unit/mL, respectively, which corresponded to the highest cell growth of 25.6 × 107 colony-forming units, observed after 6 days of incubation. Treatment of P. citri with bacterial culture caused the hydrolysis of the cuticular wax layer, chitin laminae and structural proteins of P. citri, resulting in 80.0 % mortality within 6 h after treatment. The control and PB media treatment did not cause insect mortality. The scanning electron microscopy analysis of the treated insects revealed severe cuticle degradation, with deep cracks extending from the epicuticle to the procuticle, and the erosion of the wax layer and broken setae. This study identified P. citri as an insect pest of economic importance in licorice cultivation, for the first time in the world. It also demonstrates the biopesticides potential of B. velezensis CE 100 against P. citri, through the lipolytic, chitinolytic and proteolytic activities, which could enhance licorice production and quality.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"210 ","pages":"Article 105904"},"PeriodicalIF":3.4,"publicationDate":"2025-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145358144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacillus Bsu02 and Bha48 increase wax gourd resistance to Fusarium proliferatum via multiple mechanisms 芽孢杆菌Bsu02和Bha48通过多种机制增强冬瓜对增殖镰刀菌的抗性

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biological Control

Pub Date : 2025-10-16 DOI: 10.1016/j.biocontrol.2025.105902

Huifang Zhang , Liyuan Qi , Sisheng Wang , Menghan Zhang , Zhen Liu , Yibo Zhang , Qingdi Yan , Cunjie Li , Junchang Li , Fuli Zhang

Wax gourd (Benincasa hispida) wilt has caused significant economic losses. In this study, a pathogen causing wax gourd wilt was isolated from diseased wax gourd plant and identified as Fusarium proliferatum based on morphological characteristics and molecular analysis. In order to control the disease, 62 Bacillus strains were isolated from rhizosphere soil of 6 plant species, with inhibition efficiency ranging from 21.81 % to 81.40 % at 6 days in the dual culture test. And the strains Bsu02 and Bha48 were selected for further investigation. The volatile organic compounds (VOCs) of Bsu02 and Bha48 inhibited mycelial growth of F. proliferatum with inhibition efficiency of 55.61 % and 38.05 % at 7 days, respectively. Pretreatment with either individual Bacillus strains (Bsu02 or Bha48) or their combination (Bcom) reduced wilt disease index of wax gourd seedlings, with control efficacy of 47.51 %, 44.57 %, and 37.04 %, respectively. Additionally, pretreatments with Bsu02, Bha48, and Bcom increased the activities of peroxidase (POD), phenylalanine ammonia lyase (PAL), and polyphenol oxidase (PPO) by more than 0.40-fold in wax gourd plant. These Bacillus pretreatments also upregulated the expression of pathogenesis-related (PR) genes (PR5, PR6, and PR12). Furthermore, Bacillus application significantly improved plant growth parameters, including plant height, leaf area, shoot fresh weight, and root dry weight, as well as photosynthetic parameters. Moreover, Bsu02, Bha48, and Bcom changed community compositions of rhizosphere microorganisms and increased alpha diversity of bacterial and fungal communities. In summary, Bsu02, Bha48, and Bcom increased wax gourd seedling resistance to wax gourd wilt caused by F. proliferatum via multiple mechanisms.

冬瓜（Benincasa hispida）枯萎造成重大经济损失。本研究从冬瓜病株中分离到一种引起冬瓜枯萎病的病原菌，通过形态特征和分子分析鉴定其为增生镰刀菌（Fusarium proliferatum）。为了防治该病，从6种植物根际土壤中分离出62株芽孢杆菌，在双培养试验中，6 d的抑制率为21.81% ~ 81.40%。选择菌株Bsu02和Bha48进行进一步研究。Bsu02和Bha48的挥发性有机化合物（VOCs）在7 d时对增芽霉菌丝生长的抑制率分别为55.61%和38.05%。单独处理芽孢杆菌Bsu02或Bha48或其组合处理（Bcom）均可降低冬瓜幼苗枯萎病指数，防治效果分别为47.51%、44.57%和37.04%。Bsu02、Bha48和Bcom预处理可使冬瓜过氧化物酶（POD）、苯丙氨酸解氨酶（PAL）和多酚氧化酶（PPO）活性提高0.40倍以上。这些芽孢杆菌预处理还上调了致病相关基因（PR5、PR6和PR12）的表达。此外，施用芽孢杆菌显著改善了植株的生长参数，包括株高、叶面积、茎部鲜重和根干重以及光合参数。Bsu02、Bha48和Bcom改变了根际微生物群落组成，增加了细菌和真菌群落的α多样性。综上所述，Bsu02、Bha48和Bcom通过多种机制增强了冬瓜幼苗对增芽枯叶病的抗性。

{"title":"Bacillus Bsu02 and Bha48 increase wax gourd resistance to Fusarium proliferatum via multiple mechanisms","authors":"Huifang Zhang , Liyuan Qi , Sisheng Wang , Menghan Zhang , Zhen Liu , Yibo Zhang , Qingdi Yan , Cunjie Li , Junchang Li , Fuli Zhang","doi":"10.1016/j.biocontrol.2025.105902","DOIUrl":"10.1016/j.biocontrol.2025.105902","url":null,"abstract":"<div><div>Wax gourd (Benincasa hispida) wilt has caused significant economic losses. In this study, a pathogen causing wax gourd wilt was isolated from diseased wax gourd plant and identified as Fusarium proliferatum based on morphological characteristics and molecular analysis. In order to control the disease, 62 Bacillus strains were isolated from rhizosphere soil of 6 plant species, with inhibition efficiency ranging from 21.81 % to 81.40 % at 6 days in the dual culture test. And the strains Bsu02 and Bha48 were selected for further investigation. The volatile organic compounds (VOCs) of Bsu02 and Bha48 inhibited mycelial growth of F. proliferatum with inhibition efficiency of 55.61 % and 38.05 % at 7 days, respectively. Pretreatment with either individual Bacillus strains (Bsu02 or Bha48) or their combination (Bcom) reduced wilt disease index of wax gourd seedlings, with control efficacy of 47.51 %, 44.57 %, and 37.04 %, respectively. Additionally, pretreatments with Bsu02, Bha48, and Bcom increased the activities of peroxidase (POD), phenylalanine ammonia lyase (PAL), and polyphenol oxidase (PPO) by more than 0.40-fold in wax gourd plant. These Bacillus pretreatments also upregulated the expression of pathogenesis-related (PR) genes (PR5, PR6, and PR12). Furthermore, Bacillus application significantly improved plant growth parameters, including plant height, leaf area, shoot fresh weight, and root dry weight, as well as photosynthetic parameters. Moreover, Bsu02, Bha48, and Bcom changed community compositions of rhizosphere microorganisms and increased alpha diversity of bacterial and fungal communities. In summary, Bsu02, Bha48, and Bcom increased wax gourd seedling resistance to wax gourd wilt caused by F. proliferatum via multiple mechanisms.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"210 ","pages":"Article 105902"},"PeriodicalIF":3.4,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145333193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Moth plant Araujia sericifera, an exotic smothering vine in New Zealand: Surveys for candidate biocontrol agents in South America, agent prioritization and safety testing 新西兰的一种外来窒息藤蔓蛾类植物：南美候选生物防治剂的调查、药剂优先级和安全性试验

IF 3.4 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biological Control

Pub Date : 2025-10-14 DOI: 10.1016/j.biocontrol.2025.105901

Quentin Paynter , Freda E. Anderson , Jane Barton , María Virginia Bianchinotti , Angela Bownes , Diego Carpintero , Soledad Delgado , Rolf Delhey , A.Hugh Gourlay , Lynley Hayes , Mirta Kiehr , Zane McGrath , Gustavo H. Ramírez , Daniel Testoni , C.B. Villamil , Soledad C. Villamil

Surveys in the native range of moth plant Araujia sericifera revealed a rich natural enemy biota attacking the plant, including damaging primary pathogens and root-boring and seed-feeding arthropod guilds, contrasting with surveys in New Zealand (NZ) that found only generalist defoliating and sucking arthropod herbivores and two mildly pathogenic fungi. A chrysomelid beetle with root boring larvae Freudeita cupripennis, a fruit fly Anastrepha nigrotaenia, and a defoliating rust fungus Puccinia araujiae were prioritised as potential biocontrol agents. Host-range testing indicated that all three species are sufficiently host-specific to be released in NZ. Approval was obtained to release an Argentinian population of P. araujiae and F. cupripennis, collected in Uruguay. Importation of P. araujiae into NZ was deferred until issues associated with the implementation of the Convention on Biological Diversity and associated development of Access and Benefit Sharing legislation in Argentina were overcome. It is hoped that this will proceed soon. Freudeita cupripennis was first released in 2019 and is established in NZ. An application to release A. nigrotaenia sourced from Uruguay was submitted in 2023. However, several other Anastrepha species are pests of fruit crops and some of NZ’s major fresh produce trading partners require declarations stating Anastrepha spp. are absent from NZ produce. This has delayed approval for the release of A. nigrotaenia as exporters will no longer be able to declare that the genus Anastrepha is absent from NZ if A. nigrotaenia is released to control moth plant. Consequently, trade agreements will require amendments to accommodate this.

在新西兰的调查中，仅发现了两种轻度致病真菌，而在新西兰的调查中，发现了一种丰富的天敌生物群攻击该植物，包括破坏性的初级病原体和蛀根取食的节肢动物群落。研究结果表明，具有蛀根幼虫的金体甲虫、黑斑果蝇和去叶锈菌具有潜在的生物防治潜力。宿主范围测试表明，这三个物种都具有足够的宿主特异性，可以在新西兰释放。已获批准释放在乌拉圭收集的阿根廷种群araujiae和F. cupripennis。在阿根廷克服了与执行《生物多样性公约》以及与获取和利益分享立法相关的发展有关的问题之前，推迟了向新西兰进口巴劳贾的工作。希望这将很快进行。Freudeita cupripennis于2019年首次发布，并在新西兰建立。于2023年提交了一份释放来自乌拉圭的黑棘球绦虫的申请。然而，其他几种Anastrepha是水果作物的害虫，新西兰一些主要的新鲜农产品贸易伙伴要求声明新西兰农产品中没有Anastrepha。这推迟了黑带绦虫的放行批准，因为如果黑带绦虫被释放用于控制蛾类植物，出口商将不再能够宣布Anastrepha属不在新西兰。因此，贸易协定将需要修改以适应这一点。

{"title":"Moth plant Araujia sericifera, an exotic smothering vine in New Zealand: Surveys for candidate biocontrol agents in South America, agent prioritization and safety testing","authors":"Quentin Paynter , Freda E. Anderson , Jane Barton , María Virginia Bianchinotti , Angela Bownes , Diego Carpintero , Soledad Delgado , Rolf Delhey , A.Hugh Gourlay , Lynley Hayes , Mirta Kiehr , Zane McGrath , Gustavo H. Ramírez , Daniel Testoni , C.B. Villamil , Soledad C. Villamil","doi":"10.1016/j.biocontrol.2025.105901","DOIUrl":"10.1016/j.biocontrol.2025.105901","url":null,"abstract":"<div><div>Surveys in the native range of moth plant Araujia sericifera revealed a rich natural enemy biota attacking the plant, including damaging primary pathogens and root-boring and seed-feeding arthropod guilds, contrasting with surveys in New Zealand (NZ) that found only generalist defoliating and sucking arthropod herbivores and two mildly pathogenic fungi. A chrysomelid beetle with root boring larvae Freudeita cupripennis, a fruit fly Anastrepha nigrotaenia, and a defoliating rust fungus Puccinia araujiae were prioritised as potential biocontrol agents. Host-range testing indicated that all three species are sufficiently host-specific to be released in NZ. Approval was obtained to release an Argentinian population of P. araujiae and F. cupripennis, collected in Uruguay. Importation of P. araujiae into NZ was deferred until issues associated with the implementation of the Convention on Biological Diversity and associated development of Access and Benefit Sharing legislation in Argentina were overcome. It is hoped that this will proceed soon. Freudeita cupripennis was first released in 2019 and is established in NZ. An application to release A. nigrotaenia sourced from Uruguay was submitted in 2023. However, several other Anastrepha species are pests of fruit crops and some of NZ’s major fresh produce trading partners require declarations stating Anastrepha spp. are absent from NZ produce. This has delayed approval for the release of A. nigrotaenia as exporters will no longer be able to declare that the genus Anastrepha is absent from NZ if A. nigrotaenia is released to control moth plant. Consequently, trade agreements will require amendments to accommodate this.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"210 ","pages":"Article 105901"},"PeriodicalIF":3.4,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145333191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0