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Antimicrobial effects of surface pre-reacted glass-ionomer (S-PRG) eluate against oral microcosm biofilm. 表面预反应玻璃-离子体(S-PRG)洗脱液对口腔微宇宙生物膜的抗菌效果。
IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-01 Epub Date: 2024-06-30 DOI: 10.1080/08927014.2024.2371817
Maíra Terra Garcia, Andressa Mayumi Namba, Paulo Henrique Fonseca do Carmo, Lara Luise Castro Pedroso, Patrícia Michele Nagai de Lima, Juliana Caparroz Gonçale, Juliana Campos Junqueira

This study investigated the antimicrobial activity of surface pre-reacted glass ionomer eluate (S-PRG) against oral microcosm biofilms collected from the oral cavity of patients. Dental biofilm samples were collected from three volunteers to form microcosm biofilms in vitro. Initially, screening tests were carried out to determine the biofilm treatment conditions with S-PRG eluate. The effects of a daily treatment for 5 min using three microcosm biofilms from different patients was then evaluated. For this, biofilms were formed on tooth enamel specimens for 120 h. Biofilms treated with 100% S-PRG for 5 min per day for 5 days showed a reduction in the number of total microorganisms, streptococci and mutans streptococci. SEM images confirmed a reduction in the biofilm after treatment. Furthermore, S-PRG also reduced lactic acid production. It was concluded that S-PRG eluate reduced the microbial load and lactic acid production in oral microcosm biofilms, reinforcing its promising use as a mouthwash agent.

本研究调查了表面预反应玻璃离聚物洗脱液(S-PRG)对从患者口腔收集的口腔微宇宙生物膜的抗菌活性。从三名志愿者身上采集了牙科生物膜样本,在体外形成微宇宙生物膜。首先进行筛选试验,以确定使用 S-PRG 洗脱液处理生物膜的条件。然后评估了使用来自不同患者的三个微宇宙生物膜每天进行 5 分钟处理的效果。用 100% S-PRG 处理生物膜 5 天,每天 5 分钟,结果显示总微生物数、链球菌数和变异链球菌数都有所减少。扫描电子显微镜图像证实,生物膜在处理后有所减少。此外,S-PRG 还能减少乳酸的产生。结论是,S-PRG 洗脱液减少了口腔微生态生物膜中的微生物量和乳酸的产生,从而增强了其作为漱口水制剂的应用前景。
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引用次数: 0
Characterizing the development of biofilm in polyethylene pipes in the non-chlorinated Danish drinking-water distribution system 确定丹麦无氯饮用水输水系统聚乙烯管道中生物膜的发展特征
IF 2.7 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-05-02 DOI: 10.1080/08927014.2024.2343839
Ditte A. Søborg, Bo Højris, Kurt Brinkmann, Michael R. Pedersen, Torben L. Skovhus
In newly commissioned drinking-water polyethylene (PE) pipes, biofilm develops on the inner pipe surface. The microbial community composition from colonization to the establishment of mature biofil...
在新投入使用的饮用水聚乙烯(PE)管道中,管道内表面会形成生物膜。从定植到形成成熟生物膜的微生物群落组成...
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引用次数: 0
Artificial-intelligence-model to optimize biocide dosing in seawater-cooled industrial process applications considering environmental, technical, energetic, and economic aspects. 从环境、技术、能源和经济等方面考虑,建立人工智能模型,优化海水冷却工业流程应用中的杀菌剂剂量。
IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-05-01 Epub Date: 2024-06-10 DOI: 10.1080/08927014.2024.2363241
Sergio García, David Boullosa-Falces, David S Sanz, Alfredo Trueba, Miguel Angel Gomez-Solaetxe

This research introduces an Artificial Intelligence (AI) based model designed to concurrently optimize energy supply management, biocide dosing, and maintenance scheduling for heat exchangers. This optimization considers energetic, technical, economic, and environmental considerations. The impact of biofilm on heat exchangers is assessed, revealing a 41% reduction in thermal efficiency and a 113% increase in flow frictional resistance of the fluid compared to the initial state. Consequently, the pump's power consumption, required to maintain hydraulic conditions, rises by 9%. The newly developed AI model detects the point at which the heat exchanger's performance begins to decline due to accumulating dirt, marking day 44 of experimentation as the threshold to commence the antifouling biocide dosing. Leveraging this AI model to monitor heat exchanger efficiency represents an innovative approach to optimizing antifouling biocide dosing and reduce the environmental impact stemming from industrial plants.

本研究介绍了一种基于人工智能(AI)的模型,旨在同时优化热交换器的能源供应管理、杀菌剂剂量和维护调度。这种优化考虑了能源、技术、经济和环境因素。通过评估生物膜对热交换器的影响,发现与初始状态相比,热效率降低了 41%,流体的流动摩擦阻力增加了 113%。因此,维持水力条件所需的泵功率消耗上升了 9%。新开发的人工智能模型可以检测到热交换器的性能因污垢积累而开始下降的时间点,并将实验的第 44 天作为开始添加防污杀菌剂的临界点。利用这一人工智能模型来监控热交换器的效率,是优化防污杀菌剂剂量和减少工业厂房对环境影响的创新方法。
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引用次数: 0
Bacterial adhesion and corrosion behavior of different pure metals induced by sulfate reducing bacteria. 硫酸盐还原菌诱导的不同纯金属的细菌粘附和腐蚀行为。
IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-05-01 Epub Date: 2024-06-05 DOI: 10.1080/08927014.2024.2357308
Huihai Wan, Guoqing Wang, Tiansui Zhang, Zixuan Xu, Hongfang Liu

The corrosion behaviors of four pure metals (Fe, Ni, Mo and Cr) in the presence of sulfate reducing bacteria (SRB) were investigated in enriched artificial seawater (EASW) after 14-day incubation. Metal Fe and metal Ni experienced weight losses of 1.96 mg cm-2 and 1.26 mg cm-2, respectively. In contrast, metal Mo and metal Cr exhibited minimal weight losses, with values of only 0.05 mg cm-2 and 0.03 mg cm-2, respectively. In comparison to Mo (2.2 × 106 cells cm-2) or Cr (1.4 × 106 cells cm-2) surface, the sessile cell counts on Fe (4.0 × 107 cells cm-2) or Ni (3.1 × 107 cells cm-2) surface was higher.

在富集人工海水(EASW)中培养 14 天后,研究了四种纯金属(铁、镍、钼和铬)在硫酸盐还原菌(SRB)存在下的腐蚀行为。金属铁和金属镍的重量损失分别为 1.96 毫克/厘米-2 和 1.26 毫克/厘米-2。相比之下,金属钼和金属铬的重量损失极小,分别仅为 0.05 毫克/厘米-2 和 0.03 毫克/厘米-2。与 Mo(2.2 × 106 个细胞 cm-2)或 Cr(1.4 × 106 个细胞 cm-2)表面相比,Fe(4.0 × 107 个细胞 cm-2)或 Ni(3.1 × 107 个细胞 cm-2)表面的无柄细胞数较高。
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引用次数: 0
Effective antibacterial agents in modern wound dressings: a review. 现代伤口敷料中的有效抗菌剂:综述。
IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-05-01 Epub Date: 2024-06-05 DOI: 10.1080/08927014.2024.2358913
Sadaf Torabi, Sayed Ali Hassanzadeh-Tabrizi

Wound infections are a significant concern in healthcare, leading to long healing times. Traditional approaches for managing wound infections rely heavily on systemic antibiotics, which are associated with the emergence of antibiotic-resistant bacteria. Therefore, the development of alternative antibacterial materials for wound care has gained considerable attention. In today's world, new generations of wound dressing are commonly used to heal wounds. These new dressings keep the wound and the area around it moist to improve wound healing. However, this moist environment can also foster an environment that is favorable for the growth of bacteria. Excessive antibiotic use poses a significant threat to human health and causes bacterial resistance, so new-generation wound dressings must be designed and developed to reduce the risk of infection. Wound dressings using antimicrobial compounds minimize wound bacterial colonization, making them the best way to avoid open wound infection. We aim to provide readers with a comprehensive understanding of the latest advancements in antibacterial materials for wound management.

伤口感染是医疗保健领域的一个重大问题,它导致伤口愈合时间过长。处理伤口感染的传统方法严重依赖全身性抗生素,而这与抗生素耐药菌的出现有关。因此,用于伤口护理的替代抗菌材料的开发备受关注。在当今世界,新一代伤口敷料被普遍用于伤口愈合。这些新型敷料能使伤口及其周围保持湿润,从而改善伤口愈合。然而,这种潮湿的环境也会滋生有利于细菌生长的环境。过度使用抗生素会对人类健康造成严重威胁,并导致细菌产生抗药性,因此必须设计和开发新一代伤口敷料,以降低感染风险。使用抗菌化合物的伤口敷料能最大限度地减少伤口细菌定植,是避免开放性伤口感染的最佳方法。我们旨在让读者全面了解用于伤口管理的抗菌材料的最新进展。
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引用次数: 0
Biodegradable ultrafiltration membrane enhanced with anti-biofouling agent from Anacardium occidentale extract. 使用西洋金合欢提取物中的抗生物污垢剂增强的可生物降解超滤膜。
IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-05-01 Epub Date: 2024-06-05 DOI: 10.1080/08927014.2024.2357309
Vania Septa Anggraeni, Hoong Chern Lee, Pei Sean Goh, Putu Doddy Sutrisna, Eric Wei Chiang Chan, Chen Wai Wong

Our research focuses on developing environmentally friendly biodegradable ultrafiltration (UF) membranes for small-scale water purification in areas lacking infrastructure or during emergencies. To address biofouling challenges without resorting to harmful chemicals, we incorporate bio-based extracts, such as methyl gallate from A. occidentale leaves, a Malaysian ulam herb, known for its quorum sensing inhibition (QSI) properties. The methyl gallate enriched extract was purified by solvent partitioning and integrated into cellulose-based UF membranes (0 to 7.5% w w-1) through phase inversion technique. The resulting membranes exhibited enhanced anti-organic fouling and anti-biofouling properties, with flux recovery ratio (FRR) of 87.84 ± 2.00% against bovine serum albumin and FRRs of 76.67 ± 1.89% and 69.57 ± 1.77% against E. coli and S. aureus, respectively. The CA/MG-5 membrane showed a 224% improvement in pure water flux (PWF) compared to the neat CA membrane. Our innovative approach significantly improves PWF, presenting an environmentally friendly method for biofouling prevention in UF membrane applications.

我们的研究重点是开发环境友好型生物可降解超滤膜(UF),用于缺乏基础设施地区或紧急情况下的小规模水净化。为了在不使用有害化学物质的情况下解决生物污垢问题,我们采用了生物基提取物,例如从一种马来西亚乌兰草中提取的没食子酸甲酯。富含没食子酸甲酯的提取物通过溶剂分区法纯化,并通过相反转技术整合到纤维素基超滤膜(0 至 7.5% w w-1)中。得到的膜具有更强的抗有机物堵塞和抗生物堵塞性能,对牛血清白蛋白的通量恢复比(FRR)为 87.84 ± 2.00%,对大肠杆菌和金黄色葡萄球菌的通量恢复比(FRR)分别为 76.67 ± 1.89% 和 69.57 ± 1.77%。与纯 CA 膜相比,CA/MG-5 膜的纯水通量(PWF)提高了 224%。我们的创新方法大大提高了纯水通量,为超滤膜应用中的生物污垢预防提供了一种环境友好型方法。
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引用次数: 0
The removal of pathogenic bacteria and dissolved organic matter from freshwater using microporous membranes: insights into biofilm formation and fouling reversibility 利用微孔膜去除淡水中的病原菌和溶解有机物:对生物膜形成和污垢可逆性的认识
IF 2.7 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-04-19 DOI: 10.1080/08927014.2024.2339438
Phumlile P. Mamba, Titus A. M. Msagati, Bhekie B. Mamba, Machawe M. Motsa, Thabo T. I. Nkambule
Pathogenic bacteria in drinking-water pose a health risk to consumers, as they compromise the quality of portable water. Chemical disinfection of water containing dissolved organic matter (DOM) cau...
饮用水中的致病细菌对消费者的健康构成威胁,因为它们会破坏饮用水的质量。对含有溶解有机物 (DOM) 的水进行化学消毒会导致细菌繁殖。
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引用次数: 0
Exploring the antibiofilm effects on Escherichia coli biofilm associated with colon cancer and anticancer activities on HCT116 cell line of bee products 探索蜂产品对结肠癌相关大肠杆菌生物膜的抗菌作用以及对 HCT116 细胞系的抗癌活性
IF 2.7 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-04-07 DOI: 10.1080/08927014.2024.2338106
Ayşegül Akkoyunlu, Görkem Dülger
The association between dysbiotic microbiota biofilm and colon cancer has recently begun to attract attention. In the study, the apitherapeutic effects of bee products (honey, bee venom, royal jell...
最近,菌群失调微生物群生物膜与结肠癌之间的关系开始引起人们的关注。在这项研究中,蜂产品(蜂蜜、蜂毒、蜂王浆和蜂胶)对结肠癌的抗肿瘤作用得到了证实。
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引用次数: 0
Characterization of organic fouling on thermal bubble-driven micro-pumps. 热气泡驱动微型泵上有机污垢的表征。
IF 2.7 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-03-01 Epub Date: 2024-05-24 DOI: 10.1080/08927014.2024.2353034
Brandon Hayes, Cillian Murphy, Janeth Marquez Rubio, Daimean Solis, Kaushik Jayaram, Robert MacCurdy

Thermal bubble-driven micro-pumps are an upcoming micro-actuator technology that can be directly integrated into micro/mesofluidic channels, have no moving parts, and leverage existing mass production fabrication approaches. These micro-pumps consist of a high-power micro-resistor that boils fluid in microseconds to create a high-pressure vapor bubble which performs mechanical work. As such, these micro-pumps hold great promise for micro/mesofluidic systems such as lab-on-a-chip technologies. However, to date, no current work has studied the interaction of these micro-pumps with biofluids such as blood and protein-rich fluids. In this study, the effects of organic fouling due to egg albumin and bovine whole blood are characterized using stroboscopic high-speed imaging and a custom deep learning neural network based on transfer learning of RESNET-18. It was found that the growth of a fouling film inhibited vapor bubble formation. A new metric to quantify the extent of fouling was proposed using the decrease in vapor bubble area as a function of the number of micro-pump firing events. Fouling due to egg albumin and bovine whole blood was found to significantly degrade pump performance as well as the lifetime of thermal bubble-driven micro-pumps to less than 104 firings, which may necessitate the use of protective thin film coatings to prevent the buildup of a fouling layer.

热气泡驱动微型泵是一种即将问世的微型执行器技术,可直接集成到微型/介流体通道中,没有活动部件,并可利用现有的大规模生产制造方法。这些微型泵由一个大功率微电阻器组成,它能在微秒内沸腾流体,产生高压蒸汽泡,从而执行机械功。因此,这些微型泵在微型/介流体系统(如片上实验室技术)中大有可为。然而,迄今为止,还没有研究过这些微型泵与血液和富含蛋白质的流体等生物流体之间的相互作用。在本研究中,利用频闪高速成像技术和基于 RESNET-18 迁移学习的定制深度学习神经网络,对鸡蛋白蛋白和牛全血造成的有机污垢的影响进行了表征。研究发现,污垢膜的生长抑制了气泡的形成。利用汽泡面积的减少与微泵点火次数的函数关系,提出了量化污垢程度的新指标。研究发现,鸡蛋白蛋白和牛全血造成的污垢会显著降低泵的性能,并使热气泡驱动微型泵的使用寿命缩短到 104 次以下,因此有必要使用保护性薄膜涂层来防止污垢层的形成。
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引用次数: 0
Small sea with high traffic - what is the biofouling potential of commercial ships in the Baltic Sea. 交通繁忙的小海域--波罗的海商船的生物污损潜力有多大。
IF 2.7 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-03-01 Epub Date: 2024-05-14 DOI: 10.1080/08927014.2024.2353025
Joanna Hegele-Drywa, Monika Normant-Saremba, Dagmara Wójcik-Fudalewska

Despite the Baltic Sea being one of the most intensive shipping regions in the world the potential magnitude of the biofouled hulls in this region is unknown. This study estimated the biofouling load to Baltic Sea Region (BSR) based on the wetted surface area (WSA) method with regard to country, ship type and donor bioregion. WSA flux reached 656 km2, of which 86% was associated with ships operating inside and 14% was WSA flux brought by ships from outside of the Baltic Sea. Most of the WSA was transported to Swedish, Finnish and Danish ports as well. The highest WSA flux was assigned to roll-on/roll-off, passenger and general cargo ships. The high biofouling potential in BSR indicates a potential high risk to the environment and, therefore there is an urgent need for appropriate guidelines to be introduced into daily use by the commercial shipping community.

尽管波罗的海是世界上航运最密集的地区之一,但该地区生物污损船体的潜在规模尚不清楚。本研究根据国家、船舶类型和捐献生物区域,采用湿表面积(WSA)方法估算了波罗的海地区(BSR)的生物污损负荷。湿表面积通量达到 656 平方公里,其中 86% 与在波罗的海内部作业的船只有关,14% 是船只从波罗的海外部带来的湿表面积通量。大部分 WSA 也被运往瑞典、芬兰和丹麦的港口。WSA 流量最大的是滚装船、客船和杂货船。波罗的海沿岸地区的生物污损潜力很高,这表明可能会对环境造成很大风险,因此迫切需要制定适当的准则,供商业航运界日常使用。
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引用次数: 0
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Biofouling
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