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Dual release of daptomycin and BMP-2 from a composite of β-TCP ceramic and ADA gelatin. 从 β-TCP 陶瓷和 ADA 明胶的复合材料中双重释放达托霉素和 BMP-2。
IF 3.5 3区 生物学 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-06-03 DOI: 10.1186/s12896-024-00863-4
Lucas Ritschl, Pia Schilling, Annette Wittmer, Annerose Serr, Hagen Schmal, Michael Seidenstuecker

Background: Antibiotic-containing carrier systems are one option that offers the advantage of releasing active ingredients over a longer period of time. In vitro sustained drug release from a carrier system consisting of microporous β-TCP ceramic and alginate has been reported in previous works. Alginate dialdehyde (ADA) gelatin gel showed both better mechanical properties when loaded into a β-TCP ceramic and higher biodegradability than pure alginate.

Methods: Dual release of daptomycin and BMP-2 was measured on days 1, 2, 3, 6, 9, 14, 21, and 28 by HPLC and ELISA. After release, the microbial efficacy of the daptomycin was verified and the biocompatibility of the composite was tested in cell culture.

Results: Daptomycin and the model compound FITC protein A (n = 30) were released from the composite over 28 days. A Daptomycin release above the minimum inhibitory concentration (MIC) by day 9 and a burst release of 71.7 ± 5.9% were observed in the loaded ceramics. Low concentrations of BMP-2 were released from the loaded ceramics over 28 days.

背景:含抗生素的载体系统是一种可在较长时间内释放活性成分的选择。以前的研究曾报道过由微孔 β-TCP 陶瓷和海藻酸盐组成的载体系统的体外持续药物释放。与纯海藻酸盐相比,海藻酸盐二醛(ADA)凝胶在载入β-TCP陶瓷时显示出更好的机械性能和更高的生物降解性:在第 1、2、3、6、9、14、21 和 28 天,通过 HPLC 和 ELISA 测量达托霉素和 BMP-2 的双重释放。释放后,验证了达托霉素的微生物功效,并在细胞培养中测试了复合材料的生物相容性:结果:28 天内,达托霉素和模型化合物 FITC 蛋白 A(n = 30)从复合材料中释放出来。到第 9 天时,达托霉素的释放量超过了最低抑制浓度(MIC),负载陶瓷的猝灭释放量为 71.7 ± 5.9%。在 28 天内,负载陶瓷释放了低浓度的 BMP-2。
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引用次数: 0
Activating the healing process: three-dimensional culture of stem cells in Matrigel for tissue repair 激活愈合过程:干细胞在 Matrigel 中的三维培养用于组织修复
IF 3.5 3区 生物学 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-05-25 DOI: 10.1186/s12896-024-00862-5
Shukui Xu, Liru Zhao, Yinghui Li, Xiuge Gu, Ziyang Liu, Xing Han, Wenwen Li, Wensheng Ma
To establish a strategy for stem cell-related tissue regeneration therapy, human gingival mesenchymal stem cells (hGMSCs) were loaded with three-dimensional (3D) bioengineered Matrigel matrix scaffolds in high-cell density microtissues to promote local tissue restoration. The biological performance and stemness of hGMSCs under 3D culture conditions were investigated by viability and multidirectional differentiation analyses. A Sprague‒Dawley (SD) rat full-thickness buccal mucosa wound model was established, and hGMSCs/Matrigel were injected into the submucosa of the wound. Autologous stem cell proliferation and wound repair in local tissue were assessed by histomorphometry and immunohistochemical staining. Three-dimensional suspension culture can provide a more natural environment for extensions and contacts between hGMSCs, and the viability and adipogenic differentiation capacity of hGMSCs were significantly enhanced. An animal study showed that hGMSCs/Matrigel significantly accelerated soft tissue repair by promoting autologous stem cell proliferation and enhancing the generation of collagen fibers in local tissue. Three-dimensional cell culture with hydrogel scaffolds, such as Matrigel, can effectively improve the biological function and maintain the stemness of stem cells. The therapeutic efficacy of hGMSCs/Matrigel was confirmed, as these cells could effectively stimulate soft tissue repair to promote the healing process by activating the host microenvironment and autologous stem cells.
为了建立一种干细胞相关组织再生治疗策略,在高细胞密度微组织中将人牙龈间充质干细胞(hGMSCs)与三维(3D)生物工程Matrigel基质支架负载在一起,以促进局部组织修复。通过活力和多向分化分析,研究了三维培养条件下 hGMSCs 的生物学性能和干性。建立了Sprague-Dawley(SD)大鼠全厚颊粘膜伤口模型,并将hGMSCs/Matrigel注入伤口粘膜下。通过组织形态学和免疫组化染色评估自体干细胞在局部组织中的增殖和伤口修复情况。三维悬浮培养能为 hGMSCs 的延伸和接触提供更自然的环境,hGMSCs 的活力和成脂分化能力显著增强。一项动物实验表明,hGMSCs/Matrigel 可促进自体干细胞增殖,增强局部组织胶原纤维的生成,从而显著加速软组织修复。使用 Matrigel 等水凝胶支架进行三维细胞培养,可有效改善干细胞的生物功能并保持其干性。hGMSCs/Matrigel 的疗效已得到证实,这些细胞可通过激活宿主微环境和自体干细胞,有效刺激软组织修复,促进愈合过程。
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引用次数: 0
Co-overexpression of chitinase and β-1,3-glucanase significantly enhanced the resistance of Iranian wheat cultivars to Fusarium. 几丁质酶和β-1,3-葡聚糖酶的共重表达显著增强了伊朗小麦品种对镰刀菌的抗性。
IF 3.5 3区 生物学 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-05-24 DOI: 10.1186/s12896-024-00859-0
Negin Mohammadizadeh-Heydari, Masoud Tohidfar, Bahram Maleki Zanjani, Motahhareh Mohsenpour, Rahele Ghanbari Moheb Seraj, Keyvan Esmaeilzadeh-Salestani

Fusarium head blight (FHB) is a devastating fungal disease affecting different cereals, particularly wheat, and poses a serious threat to global wheat production. Chitinases and β-glucanases are two important proteins involved in lysing fungal cell walls by targeting essential macromolecular components, including chitin and β-glucan micro fibrils. In our experiment, a transgenic wheat (Triticum aestivum) was generated by introducing chitinase and glucanase genes using Biolistic technique and Recombinant pBI121 plasmid (pBI-ChiGlu (-)). This plasmid contained chitinase and glucanase genes as well as nptII gene as a selectable marker. The expression of chitinase and glucanase was individually controlled by CaMV35S promoter and Nos terminator. Immature embryo explants from five Iranian cultivars (Arta, Moghan, Sisun, Gascogen and A-Line) were excised from seeds and cultured on callus induction medium to generate embryonic calluses. Embryogenic calluses with light cream color and brittle texture were selected and bombarded using gold nanoparticles coated with the recombinant pBI-ChiGlu plasmid. Bombarded calluses initially were transferred to selective callus induction medium, and later, they were transfferd to selective regeneration medium. The selective agent was kanamycin at a concentration of 25 mg/l in both media. Among five studied cultivars, A-Line showed the highest transformation percentage (4.8%), followed by the Sisun, Gascogen and Arta in descending order. PCR and Southern blot analysis confirmed the integration of genes into the genome of wheat cultivars. Furthermore, in an in-vitro assay, the growth of Fusarium graminearum was significantly inhibited by using 200 μg of leaf protein extract from transgenic plants. According to our results, the transgenic plants (T1) showed the resistance against Fusarium when were compared to the non-transgenic plants. All transgenic plants showed normal fertility and no abnormal response was observed in their growth and development.

镰刀菌头孢疫病(FHB)是一种毁灭性真菌疾病,影响不同谷物,尤其是小麦,对全球小麦生产构成严重威胁。几丁质酶和β-葡聚糖酶是两种重要的蛋白质,它们以几丁质和β-葡聚糖微纤维等重要的大分子成分为靶标,参与裂解真菌细胞壁。在我们的实验中,使用生物技术和重组 pBI121 质粒(pBI-ChiGlu (-))引入几丁质酶和葡聚糖酶基因,产生了转基因小麦(Triticum aestivum)。该质粒含有几丁质酶和葡聚糖酶基因以及作为可选择标记的 nptII 基因。几丁质酶和葡聚糖酶的表达分别由 CaMV35S 启动子和 Nos 终止子控制。从五个伊朗栽培品种(Arta、Moghan、Sisun、Gascogen 和 A-Line)的种子上切除未成熟胚外植体,并在胼胝体诱导培养基上培养以产生胚性胼胝体。选取颜色为浅奶油色、质地较脆的胚胎茧,使用涂有重组 pBI-ChiGlu 质粒的金纳米粒子进行轰击。轰炸后的胼胝体先转移到选择性胼胝体诱导培养基上,然后再转移到选择性再生培养基上。两种培养基中的卡那霉素浓度均为 25 毫克/升。在研究的五个栽培品种中,A-Line 的转化率最高(4.8%),其次依次是 Sisun、Gascogen 和 Arta。聚合酶链式反应和 Southern 印迹分析证实了小麦栽培品种基因组中的整合基因。此外,在体外试验中,使用 200 μg 的转基因植株叶蛋白提取物可显著抑制禾本科镰刀菌的生长。结果表明,与非转基因植物相比,转基因植物(T1)对镰刀菌具有抗性。所有转基因植株的生育力均正常,生长发育也未出现异常反应。
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引用次数: 0
A new mRNA structure prediction based approach to identifying improved signal peptides for bone morphogenetic protein 2. 一种基于 mRNA 结构预测的新方法,用于识别骨形态发生蛋白 2 的改良信号肽。
IF 3.5 3区 生物学 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-05-23 DOI: 10.1186/s12896-024-00858-1
Piers Wilkinson, Brian Jackson, Hazel Fermor, Robert Davies

Background: Signal peptide (SP) engineering has proven able to improve production of many proteins yet is a laborious process that still relies on trial and error. mRNA structure around the translational start site is important in translation initiation and has rarely been considered in this context, with recent improvements in in silico mRNA structure potentially rendering it a useful predictive tool for SP selection. Here we attempt to create a method to systematically screen candidate signal peptide sequences in silico based on both their nucleotide and amino acid sequences. Several recently released computational tools were used to predict signal peptide activity (SignalP), localization target (DeepLoc) and predicted mRNA structure (MXFold2). The method was tested with Bone Morphogenetic Protein 2 (BMP2), an osteogenic growth factor used clinically for bone regeneration. It was hoped more effective BMP2 SPs could improve BMP2-based gene therapies and reduce the cost of recombinant BMP2 production.

Results: Amino acid sequence analysis indicated 2,611 SPs from the TGF-β superfamily were predicted to function when attached to BMP2. mRNA structure prediction indicated structures at the translational start site were likely highly variable. The five sequences with the most accessible translational start sites, a codon optimized BMP2 SP variant and the well-established hIL2 SP sequence were taken forward to in vitro testing. The top five candidates showed non-significant improvements in BMP2 secretion in HEK293T cells. All showed reductions in secretion versus the native sequence in C2C12 cells, with several showing large and significant decreases. None of the tested sequences were able to increase alkaline phosphatase activity above background in C2C12s. The codon optimized control sequence and hIL2 SP showed reasonable activity in HEK293T but very poor activity in C2C12.

Conclusions: These results support the use of peptide sequence based in silico tools for basic predictions around signal peptide activity in a synthetic biology context. However, mRNA structure prediction requires improvement before it can produce reliable predictions for this application. The poor activity of the codon optimized BMP2 SP variant in C2C12 emphasizes the importance of codon choice, mRNA structure, and cellular context for SP activity.

背景:翻译起始位点周围的 mRNA 结构对翻译起始非常重要,但在这种情况下却很少被考虑,而最近对 mRNA 结构的硅学改进有可能使其成为选择信号肽的有用预测工具。在此,我们尝试创建一种方法,根据候选信号肽的核苷酸序列和氨基酸序列对其进行系统的硅学筛选。我们使用了几种最近发布的计算工具来预测信号肽活性(SignalP)、定位目标(DeepLoc)和预测的 mRNA 结构(MXFold2)。该方法用骨形态发生蛋白 2(BMP2)进行了测试,BMP2 是一种临床上用于骨再生的成骨生长因子。希望更有效的 BMP2 SPs 能改善基于 BMP2 的基因疗法,并降低重组 BMP2 的生产成本:氨基酸序列分析表明,有 2,611 个 TGF-β 超家族的 SP 与 BMP2 连接后可发挥作用。体外测试采用了五个最容易进入翻译起始位点的序列、一个经过密码子优化的 BMP2 SP 变体和一个成熟的 hIL2 SP 序列。前五名候选序列在 HEK293T 细胞中的 BMP2 分泌方面均无明显改善。与原生序列相比,所有候选序列在 C2C12 细胞中的分泌量都有所下降,其中几个序列的分泌量下降幅度大且显著。没有一个测试序列能使 C2C12 细胞中的碱性磷酸酶活性高于背景值。经过密码子优化的对照序列和 hIL2 SP 在 HEK293T 中显示出合理的活性,但在 C2C12 中活性很低:这些结果支持在合成生物学背景下使用基于肽序列的硅学工具对信号肽活性进行基本预测。然而,mRNA 结构预测需要改进,才能为这一应用提供可靠的预测结果。经过密码子优化的 BMP2 SP 变体在 C2C12 中的活性很差,这强调了密码子选择、mRNA 结构和细胞环境对 SP 活性的重要性。
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引用次数: 0
Correction: Transcriptomic and targeted metabolomic analyses provide insights into the flavonoids biosynthesis in the flowers of Lonicera macranthoides. 更正:转录组和靶向代谢组分析有助于深入了解忍冬花中黄酮类化合物的生物合成。
IF 3.5 3区 生物学 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-05-20 DOI: 10.1186/s12896-024-00860-7
Ling Ling Lv, Li Yun Li, Long Qian Xiao, Jian Hui Pi
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引用次数: 0
Nutritional composition, lipid profile and stability, antioxidant activities and sensory evaluation of pasta enriched by linseed flour and linseed oil. 亚麻籽粉和亚麻籽油富集面食的营养成分、脂质特征和稳定性、抗氧化活性及感官评价
IF 3.5 3区 生物学 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-05-15 DOI: 10.1186/s12896-024-00841-w
Zahra Amri, Amira Mnari Bhouri, Madiha Dhibi, Mohamed Hammami, Sonia Hammami, Beligh Mechri

Pasta assortments fortified with high quality foods are a modern nutritional trends. This study, explored the effects of fortification with linseed flour (LF) and linseed oil (LO) on durum wheat pasta characteristics. Wheat flour semolina was replaced with 5%, 10% and 15% of LF or 1%, 2.5% and 5% of LO. Control pasta CP (without LF or LO addition), LF-enriched pasta LFP 5%, LFP 10% and LFP 15% and LO-enriched pasta LOP 1%, LOP 2.5% and LOP 5% was compared for the proteins, fat and phenolic contents and fatty acids (FA) profile. Impact on lipid oxidation and sensory evaluation were also determined. Fortification of pasta with LF improved significantly (p < 0.05) the contents of protein, fat and phenolic compared to CP whereas the enrichment of pasta with LO resulted in a significant increase (p < 0.05) in the content of fat and a significant decrease in protein and phenolic contents. All the formulations decreased the saturated FA percent and increased the polyunsaturated FA percent with enhancement of omega-3 FA content. Antioxidant activity measured by FRAP and DPPH assays was improved after the fortification. For lipid oxidation, the replacement of semolina by LF or LO promoted an increase (p < 0.05) on TBARS values in level-dependent manner. Regarding sensory evaluation, the two types of fortification did not affect the taste; flavor and aroma of cooked pasta, but LOP 5% showed the highest score of the overall acceptability. The results recommended the possibility of producing pasta supplemented with LF or LO (even at a level of 15% and 5% respectively) as a functional food.

强化优质食品的面食是现代营养的一种趋势。本研究探讨了强化亚麻籽粉(LF)和亚麻籽油(LO)对硬质小麦面特性的影响。小麦粉中分别添加 5%、10% 和 15%的亚麻籽粉或 1%、2.5% 和 5%的亚麻籽油。比较了对照面 CP(未添加 LF 或 LO)、LF 富集面 LFP 5%、LFP 10%和 LFP 15%,以及 LO 富集面 LOP 1%、LOP 2.5%和 LOP 5%的蛋白质、脂肪、酚类含量和脂肪酸(FA)概况。此外,还测定了对脂质氧化和感官评价的影响。添加 LF 的意大利面显著提高了蛋白质含量(p
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引用次数: 0
A model approach to show that monocytes can enter microporous β-TCP ceramics. 单核细胞可进入微孔β-TCP 陶瓷的模型方法。
IF 3.5 3区 生物学 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-05-15 DOI: 10.1186/s12896-024-00857-2
Marco Waldmann, Marc Bohner, Long-Quan R V Le, Anna Baghnavi, Bianca Riedel, Michael Seidenstuecker

β-TCP ceramics are versatile bone substitute materials and show many interactions with cells of the monocyte-macrophage-lineage. The possibility of monocytes entering microporous β-TCP ceramics has however not yet been researched. In this study, we used a model approach to investigate whether monocytes might enter β-TCP, providing a possible explanation for the origin of CD68-positive osteoclast-like giant cells found in earlier works.We used flow chambers to unidirectionally load BC, PRP, or PPP into slice models of either 2 mm or 6 mm β-TCP. Immunofluorescence for CD68 and live/dead staining was performed after the loading process.Our results show that monocytes were present in a relevant number of PRP and BC slices representing the inside of our 2 mm slice model and also present on the actual inside of our 6 mm model. For PPP, monocytes were not found beyond the surface in either model.Our results indicate the possibility of a new and so far neglected constituent in β-TCP degradation, perhaps causing the process of ceramic degradation also starting from inside the ceramics as opposed to the current understanding. We also demonstrated flow chambers as a possible new in vitro model for interactions between blood and β-TCP.

β-TCP 陶瓷是一种多功能骨替代材料,可与单核-巨噬细胞系细胞发生多种相互作用。然而,单核细胞进入微孔 β-TCP 陶瓷的可能性尚未得到研究。在本研究中,我们使用模型方法研究单核细胞是否可能进入 β-TCP,为早期研究中发现的 CD68 阳性破骨细胞样巨细胞的来源提供了可能的解释。我们的结果表明,在代表 2 毫米切片模型内部的 PRP 和 BC 切片中存在一定数量的单核细胞,在 6 毫米切片模型的实际内部也存在单核细胞。我们的研究结果表明,在 β-TCP 降解过程中可能存在一种迄今为止被忽视的新成分,它可能导致陶瓷降解过程从陶瓷内部开始,而不是目前所理解的那样。我们还展示了流动室作为血液与 β-TCP 之间相互作用的一种可能的新体外模型。
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引用次数: 0
In vitro assessment of the effect of magnetic fields on efficacy of biosynthesized selenium nanoparticles by Alborzia kermanshahica. 磁场对 Alborzia kermanshahica 生物合成硒纳米粒子功效的体外评估。
IF 3.5 3区 生物学 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-05-09 DOI: 10.1186/s12896-024-00855-4
Melika Eydelkhani, Shadi Kiabi, Bahareh Nowruzi

Cyanobacteria represent a rich resource of a wide array of unique bioactive compounds that are proving to be potent sources of anticancer drugs. Selenium nanoparticles (SeNPs) have shown an increasing potential as major therapeutic platforms and led to the production of higher levels of ROS that can present desirable anticancer properties. Chitosan-SeNPs have also presented antitumor properties against hepatic cancer cell lines, especially the Cht-NP (Chitosan-NPs), promoting ROS generation and mitochondria dysfunction. It is proposed that magnetic fields can add new dimensions to nanoparticle applications. Hence, in this study, the biosynthesis of SeNPs using Alborzia kermanshahica and chitosan (CS) as stabilizers has been developed. The SeNPs synthesis was performed at different cyanobacterial cultivation conditions, including control (without magnetic field) and magnetic fields of 30 mT and 60 mT. The SeNPs were characterized by uv-visible spectroscopy, Fourier-transform infrared spectroscopy (FT-IR), Dynamic light scattering (DLS), zeta potential, and TEM. In addition, the antibacterial activity, inhibition of bacterial growth, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC), as well as the antifungal activity and cytotoxicity of SeNPs, were performed. The results of uv-visible spectrometry, DLS, and zeta potential showed that 60 mT had the highest value regarding the adsorption, size, and stabilization in compared to the control. FTIR spectroscopy results showed consistent spectra, but the increased intensity of peaks indicates an increase in bond number after exposure to 30 mT and 60 mT. The results of the antibacterial activity and the inhibition zone diameter of synthesized nanoparticles showed that Staphylococcus aureus was more sensitive to nanoparticles produced under 60 mT. Se-NPs produced by Alborzia kermanshahica cultured under a 60 mT magnetic field exhibit potent antimicrobial and anticancer properties, making them a promising natural agent for use in the pharmaceutical and biomedical industries.

蓝藻拥有丰富的资源,含有大量独特的生物活性化合物,被证明是抗癌药物的有效来源。硒纳米粒子(SeNPs)作为主要的治疗平台显示出越来越大的潜力,并导致产生更高水平的 ROS,从而呈现出理想的抗癌特性。壳聚糖-SeNPs 对肝癌细胞株也具有抗肿瘤特性,尤其是 Cht-NP(壳聚糖-NPs),可促进 ROS 生成和线粒体功能障碍。有人提出,磁场可以为纳米粒子的应用增加新的维度。因此,在本研究中,我们开发了一种以柯曼沙希子(Alborzia kermanshahica)和壳聚糖(CS)为稳定剂的 SeNPs 生物合成方法。SeNPs 的合成是在不同的蓝藻培养条件下进行的,包括对照(无磁场)以及 30 mT 和 60 mT 的磁场。通过紫外可见光谱、傅立叶变换红外光谱(FT-IR)、动态光散射(DLS)、ZETA 电位和 TEM 对 SeNPs 进行了表征。此外,还测定了 SeNPs 的抗菌活性、抑制细菌生长的能力、最低抑菌浓度(MIC)和最低杀菌浓度(MBC),以及抗真菌活性和细胞毒性。紫外可见光谱、DLS 和 zeta 电位的结果表明,与对照组相比,60 mT 在吸附、尺寸和稳定性方面的值最高。傅立叶变换红外光谱结果显示出一致的光谱,但峰值强度的增加表明在暴露于 30 mT 和 60 mT 后键数有所增加。合成纳米粒子的抗菌活性和抑菌区直径结果表明,金黄色葡萄球菌对在 60 mT 下产生的纳米粒子更敏感。在 60 mT 磁场下培养的柯曼夏鲍藻所产生的 Se-NPs 具有很强的抗菌和抗癌特性,因此是一种很有希望用于制药和生物医学行业的天然药物。
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引用次数: 0
ECM-mimetic, NSAIDs loaded thermo-responsive, immunomodulatory hydrogel for rheumatoid arthritis treatment. 用于治疗类风湿性关节炎的模拟 ECM、负载非甾体抗炎药的热响应免疫调节水凝胶。
IF 3.5 3区 生物学 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-05-09 DOI: 10.1186/s12896-024-00856-3
Dipesh Kumar Shah, Sumanta Ghosh, Namdev More, Mounika Choppadandi, Mukty Sinha, Sarath Babu Srivalliputtur, Ravichandiran Velayutham, Govinda Kapusetti

Background: Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease, and it leads to irreversible inflammation in intra-articular joints. Current treatment approaches for RA include non-steroidal anti-inflammatory drugs (NSAIDs), disease-modifying anti-rheumatic drugs (DMARDs), corticosteroids, and biological agents. To overcome the drug-associated toxicity of conventional therapy and transdermal tissue barrier, an injectable NSAID-loaded hydrogel system was developed and explored its efficacy.

Results: The surface morphology and porosity of the hydrogels indicate that they mimic the natural ECM, which is greatly beneficial for tissue healing. Further, NSAIDs, i.e., diclofenac sodium, were loaded into the hydrogel, and the in vitro drug release pattern was found to be burst release for 24 h and subsequently sustainable release of 50% drug up to 10 days. The DPPH assay revealed that the hydrogels have good radical scavenging activity. The biocompatibility study carried out by MTT assay proved good biocompatibility and anti-inflammatory activity of the hydrogels was carried out by gene expression study in RAW 264.7 cells, which indicate the downregulation of several key inflammatory genes such as COX-2, TNF-α & 18s.

Conclusion: In summary, the proposed ECM-mimetic, thermo-sensitive in situ hydrogels may be utilized for intra-articular inflammation modulation and can be beneficial by reducing the frequency of medication and providing optimum lubrication at intra-articular joints.

背景:类风湿性关节炎(RA)是一种慢性炎症性自身免疫疾病,会导致关节内出现不可逆的炎症。目前治疗类风湿性关节炎的方法包括非甾体抗炎药(NSAIDs)、改善病情抗风湿药(DMARDs)、皮质类固醇激素和生物制剂。为了克服传统疗法的药物毒性和透皮组织屏障,研究人员开发了一种可注射的装载非甾体抗炎药的水凝胶系统,并探讨了其疗效:结果:水凝胶的表面形态和孔隙率表明,它们模拟了天然 ECM,这对组织愈合大有裨益。此外,将非甾体抗炎药(即双氯芬酸钠)载入水凝胶,发现体外药物释放模式为 24 小时内猝灭释放,随后持续释放 50%的药物达 10 天。DPPH 试验表明,水凝胶具有良好的自由基清除活性。在 RAW 264.7 细胞中进行的基因表达研究表明,水凝胶具有良好的生物相容性:总之,所提出的模拟 ECM 的热敏性原位水凝胶可用于关节内炎症调节,并能减少用药次数,为关节内关节提供最佳润滑。
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引用次数: 0
High-performance internal circulation anaerobic granular sludge reactor for cattle slaughterhouse wastewater treatment and simultaneous biogas production. 用于牛屠宰场废水处理和同步沼气生产的高性能内循环厌氧颗粒污泥反应器。
IF 3.5 3区 生物学 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-05-08 DOI: 10.1186/s12896-024-00849-2
Mohamed Saad Hellal, Hala M El-Kamah, Hala Salah Doma

This research investigates the efficacy of a high-performance pilot-scale Internal Circulation Anaerobic Reactor inoculated with Granular Sludge (ICAGSR) for treating cattle slaughterhouse wastewater while concurrently generating biogas. The primary objective is to assess the efficiency and performance of ICAGSR in terms of organic pollutant removal and biogas production using granular anaerobic sludge. The research methodology entails operating the ICAGSR system under ambient conditions and systematically varying key parameters, including different Hydraulic Retention Times (HRTs) (24, 12, and 8 h) and Organic Loading Rates (OLRs) (3.3, 6.14, and 12.83 kg COD/m³. d). The study focuses on evaluating pollutants' removal and biogas production rates. Results reveal that the ICAGSR system achieves exceptional removal efficiency for organic pollutants, with Chemical Oxygen Demand (COD) removal exceeding 74%, 67%, and 68% at HRTs of 24, 12, and 8 h, respectively. Furthermore, the system demonstrates stable and sustainable biogas production, maintaining average methane contents of 80%, 76%, and 72% throughout the experimental period. The successful operation of the ICAGSR system underscores its potential as a viable technology for treating cattle slaughterhouse wastewater and generating renewable biogas. In conclusion, this study contributes to wastewater treatment and renewable energy production by providing a comprehensive analysis of the ICAGSR system's hydrodynamic properties. The research enhances our understanding of the system's performance optimization under varying conditions, emphasizing the benefits of utilizing ICAGSR reactors with granular sludge as an effective and sustainable approach. Identifying current gaps, future research directions aim to further refine and broaden the application of ICAGSR technology in wastewater treatment and renewable energy initiatives.

本研究调查了接种颗粒污泥的高性能中试规模内循环厌氧反应器(ICAGSR)在处理牛屠宰场废水并同时产生沼气方面的功效。主要目的是评估 ICAGSR 在利用颗粒状厌氧污泥去除有机污染物和产生沼气方面的效率和性能。研究方法是在环境条件下运行 ICAGSR 系统,并系统地改变关键参数,包括不同的水力停留时间 (HRT)(24、12 和 8 小时)和有机负荷率 (OLR)(3.3、6.14 和 12.83 kg COD/m³. d)。研究重点是评估污染物去除率和沼气生产率。结果表明,ICAGSR 系统对有机污染物的去除效率极高,在 24、12 和 8 小时的 HRT 条件下,化学需氧量 (COD) 的去除率分别超过 74%、67% 和 68%。此外,该系统还表现出稳定和可持续的沼气生产,在整个实验期间,平均甲烷含量分别保持在 80%、76% 和 72%。ICAGSR 系统的成功运行凸显了其作为处理牛屠宰场废水和产生可再生沼气的可行技术的潜力。总之,本研究通过对 ICAGSR 系统的流体动力学特性进行全面分析,为废水处理和可再生能源生产做出了贡献。这项研究加深了我们对该系统在不同条件下性能优化的理解,强调了利用带有颗粒污泥的 ICAGSR 反应器作为一种有效和可持续方法的益处。通过找出目前存在的差距,未来的研究方向旨在进一步完善和扩大 ICAGSR 技术在废水处理和可再生能源计划中的应用。
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