Pub Date : 2026-02-21DOI: 10.1186/s12896-026-01115-3
Huanglin Huo, Zhenhua Zhou, Rongxiang Guo, Jinyi Zhang, Menghan Li, Huicong Zeng, Dongyu Zhao, Roman P Terekhov, Peng Jiang, Qian Zhou, Bo Li
Diabetes wounds represent a significant clinical challenge, primarily attributed to their complex microenvironment characterized by persistent inflammation, heightened infection susceptibility, and impaired angiogenesis. To address these interconnected challenges, a multifunctional hydrogel dressing (designated EEP/PS) was constructed using pullulan, Brazilian propolis ethanolic extract, and polyvinyl alcohol. This biomaterial exhibits an interconnected porous structure, suitable mechanical properties, tissue adhesion, remarkable thermal stability, excellent sustained-release and swelling capacity. Notably, EEP/PS shows potent antioxidant capability and significantly enhances cell migration in hydrogen peroxide-induced oxidative injury models. Furthermore, the hydrogel downregulates the expression and secretion of key pro-inflammatory cytokines in LPS-stimulated macrophages, including tumor necrosis factor-α, interleukin-6, and interleu-kin-1β. The hydrogel also exhibits broad-spectrum antibacterial efficacy against common wound pathogens Escherichia coli and Staphylococcus aureus. In a diabetic rat model, EEP/PS accelerated wound closure by synergistically promoting reepithelialization, collagen deposition, angiogenesis while mitigating local inflammation. Additionally, EEP/PS exhibits an excellent hemostasis capacity and bio-compatibility, critical for clinical translation. Collectively, these findings highlight the translational potential of EEP/PS as a multifunctional biomaterial for diabetic wound care.
{"title":"Formulation and evaluation of hydrogel based on Brazilian propolis ethanol extract for promoting diabetic wound healing.","authors":"Huanglin Huo, Zhenhua Zhou, Rongxiang Guo, Jinyi Zhang, Menghan Li, Huicong Zeng, Dongyu Zhao, Roman P Terekhov, Peng Jiang, Qian Zhou, Bo Li","doi":"10.1186/s12896-026-01115-3","DOIUrl":"https://doi.org/10.1186/s12896-026-01115-3","url":null,"abstract":"<p><p>Diabetes wounds represent a significant clinical challenge, primarily attributed to their complex microenvironment characterized by persistent inflammation, heightened infection susceptibility, and impaired angiogenesis. To address these interconnected challenges, a multifunctional hydrogel dressing (designated EEP/PS) was constructed using pullulan, Brazilian propolis ethanolic extract, and polyvinyl alcohol. This biomaterial exhibits an interconnected porous structure, suitable mechanical properties, tissue adhesion, remarkable thermal stability, excellent sustained-release and swelling capacity. Notably, EEP/PS shows potent antioxidant capability and significantly enhances cell migration in hydrogen peroxide-induced oxidative injury models. Furthermore, the hydrogel downregulates the expression and secretion of key pro-inflammatory cytokines in LPS-stimulated macrophages, including tumor necrosis factor-α, interleukin-6, and interleu-kin-1β. The hydrogel also exhibits broad-spectrum antibacterial efficacy against common wound pathogens Escherichia coli and Staphylococcus aureus. In a diabetic rat model, EEP/PS accelerated wound closure by synergistically promoting reepithelialization, collagen deposition, angiogenesis while mitigating local inflammation. Additionally, EEP/PS exhibits an excellent hemostasis capacity and bio-compatibility, critical for clinical translation. Collectively, these findings highlight the translational potential of EEP/PS as a multifunctional biomaterial for diabetic wound care.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146775976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-21DOI: 10.1186/s12896-026-01117-1
Pfariso Maumela, Mahloro Hope Serepa-Dlamini
{"title":"In vitro and in silico analysis of the fungicidal potential of secondary metabolites from a bacterial endophyte against Botrytis cinerea.","authors":"Pfariso Maumela, Mahloro Hope Serepa-Dlamini","doi":"10.1186/s12896-026-01117-1","DOIUrl":"https://doi.org/10.1186/s12896-026-01117-1","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146776019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-21DOI: 10.1186/s12896-026-01104-6
O M O El-Maghraby, Kaoud Salama, M S Youssef, M Marwa Abdel-Kareem, A Randa Fathy
{"title":"Untargeted GC-MS metabolic profiling of Eurotium chevalieri AUMC 16390 (PX498623) reveals a putative steroidal metabolite with antibacterial and anti-inflammatory potential.","authors":"O M O El-Maghraby, Kaoud Salama, M S Youssef, M Marwa Abdel-Kareem, A Randa Fathy","doi":"10.1186/s12896-026-01104-6","DOIUrl":"10.1186/s12896-026-01104-6","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12934079/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146775958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-20DOI: 10.1186/s12896-026-01116-2
Valentin von Werz, Aleksander Szarzynski, Gregor Mattert, Sara Zigon-Branc, Werner Dammermann, Oliver Spadiut
{"title":"Cultivation strategy optimisation for NK-92 cell line expansion in static conditions.","authors":"Valentin von Werz, Aleksander Szarzynski, Gregor Mattert, Sara Zigon-Branc, Werner Dammermann, Oliver Spadiut","doi":"10.1186/s12896-026-01116-2","DOIUrl":"10.1186/s12896-026-01116-2","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12997954/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146224862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-16DOI: 10.1186/s12896-025-01098-7
Orion M Venero, Roland Ndeh, Andrew Pan, Pekka Patrikainen, Malin Eriksson, Amir Akhgari, Lauri Kakko, Daria Nedorezova, Osama Mohamed, Lia Thomson, Ann Bonde, Niina Kelanne, Eva-Mari Aro, Katarzyna P Adamala, Pauli Kallio
{"title":"CyanoConstruct: simple platform for cyanobacterial expression construct assembly and translational tuning.","authors":"Orion M Venero, Roland Ndeh, Andrew Pan, Pekka Patrikainen, Malin Eriksson, Amir Akhgari, Lauri Kakko, Daria Nedorezova, Osama Mohamed, Lia Thomson, Ann Bonde, Niina Kelanne, Eva-Mari Aro, Katarzyna P Adamala, Pauli Kallio","doi":"10.1186/s12896-025-01098-7","DOIUrl":"https://doi.org/10.1186/s12896-025-01098-7","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146206454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: The Escherichia coli expression system is widely used for recombinant protein production, but its utility is often limited by the formation of insoluble inclusion bodies. Although fusion tags can enhance solubility, their effectiveness varies unpredictably across different target proteins, and the optimal tag must typically be determined empirically.
Results: We developed a standardized set of pX fusion tag vector system for the high-throughput screening of soluble protein expression in E. coli. This system consists of eight medium-sized, TEV-cleavable fusion tags (ArsC, Crr, DsbA, Ecotin, MsyB, SlyD, Snut, and YjgD) cloned into a standardized pET-28b(+) backbone. We systematically evaluated the impact of these tags on the solubility and function of three model proteins (eGFP, EcFabG, and Mals) and six proteins known to be challenging to express in E. coli (PulA, NodE, FabF1XL, FabF2XL, FabZXL, and FabGXL). Our results demonstrated that the efficacy of each tag was highly protein-dependent. Notably, tags such as MsyB and Snut dramatically increased the soluble proportion of eGFP from 15% to over 85%, while the SlyD tag significantly enhanced both the solubility and activity of Mals. For several difficult-to-express proteins, soluble expression was only achieved with specific tags, highlighting the critical importance of tag selection.
Conclusions: Our study presents a versatile and efficient parallel cloning and screening system for the rapid production of soluble recombinant proteins. By enabling parallel screening of multiple fusion partners, this system facilitates the identification of optimal conditions for enhancing protein solubility and function, thereby addressing a key bottleneck in recombinant protein applications.
{"title":"Application of a novel fusion tag system for enhanced soluble expression of recombinant proteins in Escherichia coli.","authors":"Li-Zhen Luo, Jian-Tao Cai, Zi-Ying Tan, Yu-Qing Chen, Zhe Hu, Hai-Hong Wang, Wen-Bin Zhang, Jin-Cheng Ma","doi":"10.1186/s12896-026-01109-1","DOIUrl":"10.1186/s12896-026-01109-1","url":null,"abstract":"<p><strong>Background: </strong>The Escherichia coli expression system is widely used for recombinant protein production, but its utility is often limited by the formation of insoluble inclusion bodies. Although fusion tags can enhance solubility, their effectiveness varies unpredictably across different target proteins, and the optimal tag must typically be determined empirically.</p><p><strong>Results: </strong>We developed a standardized set of pX fusion tag vector system for the high-throughput screening of soluble protein expression in E. coli. This system consists of eight medium-sized, TEV-cleavable fusion tags (ArsC, Crr, DsbA, Ecotin, MsyB, SlyD, Snut, and YjgD) cloned into a standardized pET-28b(+) backbone. We systematically evaluated the impact of these tags on the solubility and function of three model proteins (eGFP, EcFabG, and Mals) and six proteins known to be challenging to express in E. coli (PulA, NodE, FabF1XL, FabF2XL, FabZXL, and FabGXL). Our results demonstrated that the efficacy of each tag was highly protein-dependent. Notably, tags such as MsyB and Snut dramatically increased the soluble proportion of eGFP from 15% to over 85%, while the SlyD tag significantly enhanced both the solubility and activity of Mals. For several difficult-to-express proteins, soluble expression was only achieved with specific tags, highlighting the critical importance of tag selection.</p><p><strong>Conclusions: </strong>Our study presents a versatile and efficient parallel cloning and screening system for the rapid production of soluble recombinant proteins. By enabling parallel screening of multiple fusion partners, this system facilitates the identification of optimal conditions for enhancing protein solubility and function, thereby addressing a key bottleneck in recombinant protein applications.</p>","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13005367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146177396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-12DOI: 10.1186/s12896-025-01073-2
Sakine Sokhtanlo, Nasrin Moshtaghi, Abdolreza Bagheri, Ahmad Sharifi, Sonia Jodeir
{"title":"Wheat transcriptome changes in response to the black stem rust pathogen (Puccinia graminis f. sp. tritici).","authors":"Sakine Sokhtanlo, Nasrin Moshtaghi, Abdolreza Bagheri, Ahmad Sharifi, Sonia Jodeir","doi":"10.1186/s12896-025-01073-2","DOIUrl":"10.1186/s12896-025-01073-2","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13005552/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146177582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-10DOI: 10.1186/s12896-026-01106-4
Shiyu Zhang, Fan Du, Ye Liao, Chuyu Zhang, Meixia Zhang
{"title":"Forsythiaside A inhibits the progression of intrahepatic cholangiocarcinoma partially via suppressing oxidative stress-induced hepatic stellate cell activation and macrophage polarization.","authors":"Shiyu Zhang, Fan Du, Ye Liao, Chuyu Zhang, Meixia Zhang","doi":"10.1186/s12896-026-01106-4","DOIUrl":"10.1186/s12896-026-01106-4","url":null,"abstract":"","PeriodicalId":8905,"journal":{"name":"BMC Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12983751/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146155926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号