BMC Biotechnology最新文献

The current study aims to assess the efficiency of different irradiated potassium sources as alternatives to ethrel in enhancing the fruit quality and storability of Ruby grapevines. This investigation was conducted on seven- year- old Ruby Seedless grape cultivars that were sprayed as follows: control (water); ethrel at 150 ppm; potassium citrate at 2000 and 4000 ppm; irradiated potassium citrate at 750 and 1500 ppm; potassium silicate at 2000 and 4000 ppm and irradiated potassium silicate at 750 and 1500 ppm. The data revealed that all the potassium sprays used significantly increased cluster weight, berry weight, and berry firmness compared with those in the control and ethrel groups. Moreover, the total soluble solids, sugars and anthocyanin contents increased with decreasing acidity% compared with those of the control group. Storability data revealed that all potassium sprays decreased weight loss, decay, firmness loss, and shattering during the storage period compared with those of the control and ethrel groups. Additionally, the total soluble solids, sugars and anthocyanin contents increased with decreasing acidity compared with those of the control. In conclusion, potassium sprays, especially irradiated potassium silicate, can be a good alternative to ethrel for improving the physical and chemical properties of Ruby Seedless grapes and increasing their storability by improving firmness and reducing weight loss, decay, and shattering.

This study aims to evaluate the effectiveness of a new alternative biofungicidal product in controlling the fungal disease agent, Fusarium oxysporum f. sp. cepae, which causes considerable losses during onion storage. In the first stage of this study, silver nanoparticles derived from the microorganism (Phoma herbarum) were biosynthesized using the green synthesis method in nanotechnology. Characterisation tests of the nano product, which was synthesized through the biosynthesis process, confirmed that it is a silver-based nano product with size dimensions ranging from 4 to 40 nm. In the second phase of the study, the fungicidal efficacy of the synthesized silver nanoparticle product, at concentrations ranging from 2.5 to 500 ppm, was investigated against pathogenic fungi. Its effectiveness was evaluated under both in vitro and in vivo conditions. In the in vitro study conducted in petri dishes, the highest mycelial growth, 87.66 ± 0.57 mm, was observed in the control group. Following the application of 2.5 and 5 ppm silver nanoparticles, the mycelium diameter measured 86.66 ± 1.15 mm. However, as the concentration of silver nanoparticles increased, a significant reduction in mycelial growth was observed. At 200 ppm, the mycelial diameter decreased to 10.66 ± 1.15 mm; at 500 ppm, no mycelial growth was observed. In the in vivo study conducted on onion bulbs, the fungicidal activity of silver nanoparticles was evaluated by measuring the disease inhibition rates after the application of silver nanoparticles on the bulbs. When disease inhibition rates are compared in onion bulbs, the dose-dependent rot diameters are as follows: positive control(50.20 ± 0.20 mm); 10 ppm (34.60 ± 0.50 mm); 25 ppm (27.60 ± 0.30 mm); 50 ppm (19.00 ± 0.38); 100 ppm (12.00 ± 0.40); 200 ppm (6.80 ± 0.31 mm) and 500 ppm (1.20 ± 0.17 mm). In addition to these results, different concentrations of silver nanoparticles demonstrated a dose-dependent reduction in disease incidence, ranging from 28.89% to 94.42%. When evaluated collectively, the results indicated that silver nanoparticles synthesized through the green synthesis method exhibited varying degrees of fungicidal activity under both in vitro and in vivo conditions, with effectiveness dependent on the concentration applied. Consequently, the synthesized silver nanoparticles product has demonstrated considerable potential as an alternative solution for effectively managing Fusarium oxysporum f. sp. cepae a significant threat to stored onion bulbs.

Background: Fruit peels as a lignocellulosic biomass are rich in sugars and nutrients, thus making them favorable medium for yeast growth and various materials production. This study showed the potential of fuit peels hydrolysate (FPH) as a substrate for single-cell protein (SCP) and bioethanol production, depending on substrate concentration, pH, oxygen availability.

Results: The highest specific growth rate (0.50 ± 0.01 h^- 1) was observed at pH 6.5 in non-diluted hydrolysates, which corresponds to ~ 250.0 g L^- 1 carbohydrates and 0.2 g L^- 1 total nitrogen concentration. The maximal protein production yield (~ 50% by dry weight) was recorded during 24 h cultivation of S. cerevisiae ATCC 13,007 under oxygen-limited conditions at pH 6.5, while the maximal ethanol production (109.00 ± 4.00 g L^- 1) was observed at 6 h for S. cerevisiae ATCC 9804 and ATCC 13,007 starins under aerobic conditions. The maximal fermentation efficiency (~ 99%) was observed at 24 h cultivation of S. cerevisiae ATCC 13,007 in 2-fold diluted hydrolysate under oxygen-limited conditions at pH 6.5, while the same strain exhibited the highest carbon conversion efficiency (CCE) (~ 56%) during aerobic cultivation in 2-fold diluted hydrolysate at pH 3.0.

Conclusion: Thus, FPH can serve as a favorable substrate for SCP and bioethanol production using S. cerevisiae strains ATCC 13,007 and ATCC 9804. Nitrogen supplementation and combination with fruit and vegetable processing industries could further enhance SCP production and process efficiency which aligns with circular economic strategy.