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Leaf microstructure and photosynthetic characteristicsof a rice midvein-deficient mutant dl-14 水稻中脉缺乏突变体dl-14的叶片微观结构和光合特性
IF 1.5 4区 生物学 Q4 PLANT SCIENCES Pub Date : 2022-07-18 DOI: 10.32615/bp.2022.007
G. Kang, N. Zhang, T. H. Tan, Z. Zhang, R. Wang, Lin-Tao Wu
of the light-adapted state; F m - maximal fluorescence; F m ' - maximal fluorescence yield of the light-adapted state; F v /F m - maximal quantum yield of PS II photochemistry; F v '/F m ' - efficiency of excitation capture of open PS II center in the light conditions; F s - fluorescence intensity at any time; NPQ - nonphotochemical quenching coefficient; PAR - photosynthetically active radiation; P N - net photosynthetic rate; PS - photosystem; q P - photochemical quenching coefficient; WUE - water use efficiency; Φ PSII - effective quantum yield of PS II photochemistry. Abstract Midvein is an important structure of the upright leaf of rice, and its normal development is essential to the formation of a common plant type of rice ( Oryza sativa L.). To reveal the effect of midvein deficiency on photosynthesis-related characteristics, leaf microstructure, and vein characteristics, the photosynthetic features between the midvein-deficient mutant dl-14 and wild-type Huanghuazhan plants were analyzed. The results indicated that the midvein area of the dl-4 mutant lacked large intercellular space and instead it was filled with mesophyll cells. Moreover, the vein density of the dl-14 mutant was significantly higher than that in cv. Huanghuazhan. Chlorophyll (Chl) a , Chl b , and carotenoid content were markedly elevated in dl-14 . In terms of photosynthetic characteristics, we observed that under high irradiance and high CO 2 concentration, the net photosynthetic rate of dl-14 plants was significantly higher than that of Huanghuazhan plants, but its water use efficiency was significantly lower. In addition, several major photosynthetic parameters, including characteristics of chlorophyll fluorescence (the efficiency of excitation capture of open PS II center, photochemical quenching, effective quantum yield of PS II photochemistry, and electron transfer rate) were significantly higher in dl-14 plants compared to Huanghuazhan plants, but the nonphotochemical quenching of dl-14 mutant was significantly lower than that of Huanghuazhan. These findings indicate that the dl-14 mutant has higher vein density, stronger photon conversion ability, and weaker radiation dissipation ability. This study can provide theoretical support for breeders to use the midvein-deficient mutant.
光适应状态;Fm——最大荧光;Fm’-光适应状态的最大荧光产率;Fv/Fm——PSⅡ光化学的最大量子产率;F v’/F m’——光照条件下开放PS II中心的激发捕获效率;Fs——任何时间的荧光强度;NPQ——非光化学猝灭系数;标准杆数——光合活性辐射;净光合速率;PS-光系统;q P——光化学猝灭系数;WUE——用水效率;ΦPSII——PSⅡ光化学的有效量子产率。摘要中脉是水稻直立叶的重要结构,其正常发育对普通株型水稻(Oryza sativa L.)的形成至关重要,分析了中脉缺失突变体dl-14与野生型黄花展植株的光合特性。结果表明,dl-4突变体的中脉区缺乏较大的细胞间隙,而是充满了叶肉细胞。此外,dl-14突变体的静脉密度显著高于黄花展。dl-14中叶绿素a、叶绿素b和类胡萝卜素含量显著升高。在光合特性方面,我们观察到,在高光照和高CO2浓度下,dl-14植物的净光合速率显著高于黄花展植物,但其水分利用效率显著较低。此外,与黄花展植物相比,dl-14植物的几个主要光合参数,包括叶绿素荧光特性(开放PS II中心的激发捕获效率、光化学猝灭、PS II光化学的有效量子产率和电子转移速率)显著更高,但dl-14突变体的非光化学猝灭显著低于黄花展突变体。这些发现表明,dl-14突变体具有更高的静脉密度、更强的光子转换能力和较弱的辐射耗散能力。本研究可为育种人员利用中脉缺陷突变体提供理论支持。
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引用次数: 0
Advances in the application of biosynthesis and metabolic engineering of flavonoids in plants 黄酮类化合物生物合成和代谢工程在植物中的应用进展
IF 1.5 4区 生物学 Q4 PLANT SCIENCES Pub Date : 2022-06-25 DOI: 10.32615/bp.2022.014
Y. Wang, Peng Li, L. Yao, Y. Shang, S. Liu, J. Meng, S. Zhang, H.H. Li
Flavonoids are secondary metabolites widely distributed in plants. They not only confer a wide spectrum of pigmentation to plant flowers but also protect plants from various biotic and abiotic stresses. Simultaneously, these compounds also offer health benefits to humans. Significant efforts have been made to correlate specific flavonoid production with biosynthetic pathway gene expression. Some structure genes and transcription factors that regulate the biosynthetic pathway have been identified. However, the diverse and complex control of flavonoid accumulation is still not well understood. In this mini-review, we summarized the improvement of flavonoids by genetic engineering from the aspects of flower colour, plant resistance, and benefits on the human diet. A perspective on flavonoid research in plants is provided.
黄酮类化合物是植物中广泛分布的次生代谢产物。它们不仅赋予植物花朵广泛的色素沉着,还保护植物免受各种生物和非生物胁迫。同时,这些化合物也对人类健康有益。已经做出了重大努力来将特定的类黄酮产生与生物合成途径基因表达联系起来。一些调节生物合成途径的结构基因和转录因子已被鉴定。然而,类黄酮积累的多样性和复杂性控制仍然没有得到很好的理解。在这篇综述中,我们从花色、植物抗性和对人类饮食的益处等方面总结了基因工程对黄酮类化合物的改良。对植物黄酮类化合物的研究进行了展望。
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引用次数: 3
Amino acid metabolism and expression of genes involved in nitrogen assimilation in common oranges cv. Valencia Late 普通橙子氨基酸代谢及氮同化相关基因的表达。瓦伦西亚晚
IF 1.5 4区 生物学 Q4 PLANT SCIENCES Pub Date : 2022-06-03 DOI: 10.32615/bp.2022.004
S. Fabroni, M. Amenta, S. Rapisarda, B. Torrisi, C. Licciardello
Numerous studies have been conducted to evaluate the impact of organic and conventional production systems on nutrients, antioxidant substances, and N-containing components in fruits and vegetables (Brandt and Molgaard 2001, Carbonaro and Mattera 2001, Rapisarda et al. 2005 and 2010, Del Amor et al. 2008, Esch et al. 2010, Rosen 2010, Camin et al. 2011, Chebrolu et al. 2012). Ecological and agronomic studies on the influence of fertilization on plant metabolism have shown that there is an inverse relationship in plants between the available N and the accumulation of defence-related secondary metabolites such as vitamin C (Brandt et al. 2011). It has been shown that changes in N status play an important role in the secondary metabolism of plants, in which nitrogen deficiency leads to a marked shift from the N-containing compounds to the production of carbon-rich defencerelated phenylpropanoids (Leser and Treutter 2005, Toor et al. 2006, Benbrook et al. 2008, Nguyen and Niemeyer
已经进行了大量研究来评估有机和传统生产系统对水果和蔬菜中营养素、抗氧化物质和含氮成分的影响(Brandt和Molgaard 2001,Carbonaro和Mattera 2001,Rapisarda等人2005和2010,Del Amor等人2008,Esch等人2010,Rosen 2010,Camin等人2011,Chebrolu等人2012)。关于施肥对植物代谢影响的生态学和农学研究表明,植物中的有效氮与防御相关次生代谢产物(如维生素C)的积累之间存在反比关系(Brandt等人,2011)。研究表明,氮状态的变化在植物的二次代谢中起着重要作用,在二次代谢过程中,氮缺乏导致从含氮化合物向产生富含碳的防御性苯丙素的显著转变(Leser和Treutter 2005,Toor等人2006,Benbrook等人2008,Nguyen和Niemeyer
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引用次数: 2
Expression of genes encoding terpenoid biosynthesis enzymes during leaf development of Eucalyptus camaldulensis 茶树叶片发育过程中萜类生物合成酶编码基因的表达
IF 1.5 4区 生物学 Q4 PLANT SCIENCES Pub Date : 2022-05-26 DOI: 10.32615/bp.2021.073
N. Zhan, L. Huang, Z. Wang, J. Zhang, Y. Xie, X. Shang, G. Liu, Z. Wu
- gas chromatography-mass spectrometry; GO - gene ontology; HMGCS - hydroxymethylglutaryl-CoA synthase; IPP - isoprene phosphate; KEGG - Kyoto encyclopedia of genes and genomes; ME-CPP - 2-C-methyl-D-erythritol- 2,4-cyclodiphosphate synthase; MEP - methylerythritol 4-phosphate; MVA - mevalonate; RIN - RNA integrity number; RPKM - reads per kb per million reads; TPS - terpene synthase; TFs - transcription factors. Abstract To reveal the regulation mechanism of terpenoid biosynthesis in the leaves of Eucalyptus camaldulensis , the content of volatiles in eucalyptus leaves and the transcriptome databases of young and mature leaves were analyzed. The results showed that E. camaldulensis contains 92 and 89 kinds of volatile substances in the young and mature leaves, respectively. Among them, the content of 1,8-cineole, β-pinene, and other substances was significantly different in young and mature leaves. A total of 99 802 unigenes were obtained from the transcriptome database of young and mature leaves of E. camaldulensis and 18 441 genes displayed obviously differential expressions during both developmental stages. There were 6 982 up-regulated unigenes and 11 461 down-regulated unigenes in the young leaf stage compared to the mature leaf stage. The key genes for terpenoid biosynthesis, including limonene synthase-10, limonene synthase-11, myrcene synthase-1, α- pinene synthase-2, and 1,8-cineole synthase-2 , were selected for further analysis to explore the mechanism of gene regulation and genetic transformation. The expressions of key genes were validated by RT-qPCR, and their expressions were consistent with RNA-seq data. WRKY, MYB, NAC , and bHLH transcription factors (TFs) displayed important regulatory effects on the above key genes. Thus, a regulatory network model of terpenoid biosynthesis was constructed using target genes and TFs during leaf development in E. camaldulensis . These results provide theoretical evidence for understanding the terpenoid biosynthesis in plants and reference for terpenoids utilization by genetic engineering methods in E. camaldulensis .
-气相色谱-质谱法;GO -基因本体;羟甲基戊二酰辅酶a合成酶;IPP -磷酸异戊二烯;KEGG -京都基因和基因组百科全书;ME-CPP - 2- c -甲基- d -赤藓糖醇- 2,4-环二磷酸合成酶;MEP - 4-磷酸甲基赤藓糖醇;MVA -甲羟戊酸;RIN - RNA完整性数;RPKM——每百万读每kb的读数;TPS -萜烯合成酶;转录因子。摘要为揭示茶树叶片萜类生物合成的调控机制,对桉树叶片挥发物含量及幼叶和成熟叶转录组数据库进行了分析。结果表明,山梨树幼叶和成熟叶中挥发物含量分别为92种和89种。其中,1,8-桉树脑、β-蒎烯等物质的含量在幼叶和成熟叶中差异显著。从茶树幼叶和成熟叶转录组数据库中共获得99 802个基因,其中18 441个基因在两个发育阶段表现出明显的差异表达。与成熟叶期相比,幼叶期有6 982个上调基因,11 461个下调基因。选择萜类生物合成的关键基因柠檬烯合成酶-10、柠檬烯合成酶-11、月桂烯合成酶-1、α-蒎烯合成酶-2和1,8-桉树脑合成酶-2进行进一步分析,探讨基因调控和遗传转化的机制。通过RT-qPCR验证了关键基因的表达,其表达与RNA-seq数据一致。WRKY、MYB、NAC和bHLH转录因子对上述关键基因均有重要的调控作用。因此,利用靶基因和TFs构建了茶树叶片发育过程中萜类生物合成的调控网络模型。这些结果为了解植物中萜类化合物的生物合成过程提供了理论依据,并为利用基因工程方法利用山核桃中的萜类化合物提供了参考。
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引用次数: 0
Effects of high growth-medium temperature under controlled conditions on characteristics of tomato leaves 控制条件下高生长培养基温度对番茄叶片特性的影响
IF 1.5 4区 生物学 Q4 PLANT SCIENCES Pub Date : 2022-05-11 DOI: 10.32615/bp.2021.070
J. Salem, S. Smiti, M. Petřivalský
PRX peroxidase; ROS reactive oxygen species; SOD superoxide dismutase; TBA thiobarbituric acid; TCA trichloroacetic acid. Abstract High temperatures have become a major threat that seriously affects crop growth and yield. The present work aimed to investigate the acclimation process in adjusting plant responses to high root temperatures. Tomato ( Solanum lycopersicum L., cv. Micro-Tom) during the flowering time was subjected to heat treatments (day/night temperatures at the root level of 40 or 45 °C for 4 d) while control plants were maintained at 25 °C, and the heat-stress treatment effects were analysed in the tomato leaves. The results showed a reduction in the content of chlorophylls a and b as well as chlorophyll a / b ratio at both high temperatures. Further, the increase in the amount of malondialdehyde as an indicator of lipid peroxidation was greater at 45 °C. The leaf content of hydrogen peroxide was induced in tomato plants subjected to 45 °C whereas it was markedly decreased in plants maintained at 40 °C as compared to control plants. Antioxidant enzymes showed higher activity in tomatoes treated at 45 °C compared to those treated at 40 °C. Moreover, the highest amount of antioxidants such as carotenoids and ascorbate in tomato plants were found at a temperature of 45 °C. Collectively, we provide evidence that physiological and biochemical components can be altered depending on the heat level, exposure time, and developmental stage. The interaction of root and shoot under high temperatures must be further characterized in terms of understanding the challenging climate changes.
PRX过氧化物酶;ROS活性氧;SOD超氧化物歧化酶;硫代巴比妥酸;TCA三氯乙酸。摘要高温已成为严重影响作物生长和产量的主要威胁。本工作旨在研究植物对高温根系反应的适应过程。番茄(Solanum lycopersicum L.,cv.Micro-Tom)在开花期间接受热处理(昼夜温度为40或45°C,持续4天),而对照植物则保持在25°C,并分析番茄叶片中的热胁迫处理效果。结果表明,在两种高温下,叶绿素a和b的含量以及叶绿素a/b的比例都有所降低。此外,作为脂质过氧化指标的丙二醛含量在45°C时增加更大。与对照植物相比,在45°C的番茄植株中,过氧化氢的叶片含量被诱导,而在40°C的植株中,过氧化物的叶片含量显著降低。与40°C处理的番茄相比,45°C处理番茄的抗氧化酶活性更高。此外,番茄植物中抗氧化剂(如类胡萝卜素和抗坏血酸)的含量在45°C的温度下最高。总之,我们提供的证据表明,生理和生化成分可以根据热量水平、暴露时间和发育阶段而改变。在理解具有挑战性的气候变化方面,必须进一步表征高温下根系和地上部的相互作用。
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引用次数: 0
In vivo assessment of salinity stress tolerance in transgenic Arabidopsis plants expressing Solanum tuberosum D200 gene 表达龙葵D200基因的转基因拟南芥耐盐性体内评价
IF 1.5 4区 生物学 Q4 PLANT SCIENCES Pub Date : 2022-04-25 DOI: 10.32615/bp.2021.072
M. Gururani
Transgenic Arabidopsis plants expressing a potato D200 gene encoding a hypothetical protein were subjected to salinity stress and assessed for their tolerance. The D200 Arabidopsis lines exhibited increased chlorophyll content, improved stomatal conductance, less electrolyte leakage, lower accumulation of malondialdehyde (MDA), and a higher amount of proline compared to the wild type (WT) plants under salinity stress. The gene expression analysis revealed that D200 plants accumulated a significantly higher amount of mRNA transcripts of genes encoding three major antioxidant enzymes ascorbate peroxidase (APX), catalase (CAT), and superoxide dismutase (SOD). Chlorophyll a fluorescence kinetics analyses showed the D200 plants were more efficient in terms of primary photochemistry of photosystem II and performance indices. Furthermore, the quantum yields and efficiencies that represent the critical steps of photosynthetic light reactions were analyzed and it was found that D200 plants were photosynthetically more active than the WT plants under salt stress conditions. Overall, these findings suggest that the D200 gene is a potential candidate gene for developing stress-resilient crops in future.
表达马铃薯D200基因编码一种假设蛋白的转基因拟南芥植株经受了盐度胁迫,并评估了它们的耐受性。与野生型(WT)相比,D200拟南芥品系在盐胁迫下叶绿素含量增加,气孔导度改善,电解质泄漏减少,丙二醛(MDA)积累减少,脯氨酸含量增加。基因表达分析显示,D200植株的抗坏血酸过氧化物酶(APX)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)三种主要抗氧化酶基因mRNA转录量显著增加。叶绿素a荧光动力学分析表明,D200植株在光系统II初级光化学和性能指标方面效率更高。此外,对代表光合光反应关键步骤的量子产率和效率进行了分析,发现在盐胁迫条件下,D200植株的光合活性高于WT植株。总之,这些发现表明,D200基因是未来培育抗逆性作物的潜在候选基因。
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引用次数: 2
Genome-wide identification and expression analysis of the AhTrx family genes in peanut 花生AhTrx家族基因的全基因组鉴定与表达分析
IF 1.5 4区 生物学 Q4 PLANT SCIENCES Pub Date : 2022-04-16 DOI: 10.32615/bp.2021.077
X. Li, G. Su, A. Ntambiyukuri, B. Tong, J. Zhan, A. Q. Wang, D. Xiao, L-F. He
Thioredoxins (Trx) are small multifunctional redox proteins that contain thioredoxin conserved domain and active site WCXXC. The Trx family has an important role in multiple processes, including electron transport, seed germination, redox regulation, biotic and abiotic stresses resistance, etc . Although Trx genes have been extensively characterized in some plants, they have not been reported in peanut until now. The identification of AhTrx genes provides potential candidate genes for studying their effects and regulatory mechanisms in peanut ( Arachis hypogaea L.) growth and development, especially under aluminium (Al) stress. It is also helpful to further analyze the Al resistance pathway in plants. Seventy AhTrx genes were identified using a genome-wide search method and conservative domain analysis. Then the basic physicochemical properties, phylogenetic relationship, gene structure, chromosomal localization, and promoter prediction were studied by the bioinformatic methods. Furthermore, the expressions of AhTrx genes under different Al treatment times in two peanut cultivars were tested using a real-time quantitative polymerase chain reaction. Seventy AhTrx genes were identified and characterized. Phylogenetic tree analysis showed that all AhTrx members could be classified into 9 groups with different conserved domains. Motif 1 was found to exist in every sequence, with an active site. Furthermore, the gene structures showed that the AhTrx family was complicated and changeable during evolution. The chromosomal localization indicated that the distribution and density of the Trx family on 20 peanut chromosomes were uneven. Predictive promoter analysis indicated that AhTrx proteins might play a role in phytohormones synthesis and stress response. Finally, the expression patterns of the AhTrx genes showed that every gene was differently expressed under Al treatment in different peanut cultivars, some were obvious, others had no significant difference, some were at a high level, while others were at a low level. This study systematically identifies the Trx gene family in peanut, providing some candidates for further study on its effects and regulatory mechanism under Al stress in peanut.
硫氧还蛋白(Thioredoxins, Trx)是一种含有硫氧还蛋白保守结构域和活性位点WCXXC的小型多功能氧化还原蛋白。Trx家族在电子传递、种子萌发、氧化还原调控、生物和非生物胁迫抗性等多个过程中发挥重要作用。Trx基因在一些植物中已被广泛鉴定,但在花生中尚未见报道。AhTrx基因的鉴定为研究其在花生(Arachis hypogaea L.)生长发育特别是铝胁迫下的作用和调控机制提供了潜在的候选基因。这也有助于进一步分析植物抗铝途径。使用全基因组搜索方法和保守结构域分析鉴定了70个AhTrx基因。然后用生物信息学方法对其基本理化性质、系统发育关系、基因结构、染色体定位和启动子预测进行了研究。利用实时定量聚合酶链反应技术检测了两个花生品种在不同铝处理时间下AhTrx基因的表达。鉴定鉴定了70个AhTrx基因。系统进化树分析表明,所有AhTrx成员可分为9个具有不同保守结构域的类群。Motif 1在每个序列中都存在,并有一个活性位点。此外,基因结构表明AhTrx家族在进化过程中是复杂多变的。染色体定位表明,Trx家族在花生20条染色体上的分布和密度不均匀。预测启动子分析表明,AhTrx蛋白可能在植物激素合成和胁迫反应中发挥作用。AhTrx基因的表达谱表明,不同花生品种在Al处理下各基因的表达量存在差异,有的表达量显著,有的表达量不显著,有的表达量高,有的表达量低。本研究系统鉴定了花生中Trx基因家族,为进一步研究其在铝胁迫下对花生的作用及调控机制提供了候选基因。
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引用次数: 0
Regulation of StTCP15 gene expression and tuber dormancy characteristics of potato by gibberellic acid, abscisic acid, and low temperature 赤霉酸、脱落酸和低温对马铃薯StTCP15基因表达和块茎休眠特性的调控
IF 1.5 4区 生物学 Q4 PLANT SCIENCES Pub Date : 2022-04-07 DOI: 10.32615/bp.2021.065
Y. Che, Yueyue Liao, Xue Fu, Y. Yue, N. Zhang, H. Si
Potato ( Solanum tuberosum L.) cv. Eshu 10 was used to investigate the effects of exogenous gibberellic acid (GA 3 ), abscisic acid (ABA), and low-temperature stress on changes of hormone content, expression patterns of StTCP15 gene, and tuber dormancy characteristics. Under GA 3 treatment and low-temperature stress, tuber dormancy was broken in about one week sooner compared with the control group, but ABA treatment did not significantly promote the breaking of tuber dormancy. The results of hormone determination using liquid chromatography-mass spectrometry (LC-MS/ MS) showed that the content of ABA in tubers treated with GA 3 or low-temperature stress was lower than in the control group, and it was higher than in the control group under ABA treatment. The GA 3 content of tubers was higher than in the control group under GA 3 treatment and lower under low-temperature stress. During dormancy, the ABA content continued to increase and GA 3 content fluctuated, ABA content rapidly decreased and GA 3 content rapidly increased when the dormancy was breaking, and both ABA content and GA 3 content increased during germination. The results from the assay of real-time quantitative PCR showed that the expression of the StTCP15 gene was continuously increased during the dormant period in all groups, and the expression of the StTCP15 gene was the highest at the time of dormancy release. The expression of the StTCP15 gene was increased about 15 times on the 7 th d under low-temperature stress and was restored at room temperature. Thus, the StTCP15 gene can respond to GA 3 , ABA, and low-temperature stress and may be involved in the release of potato tuber dormancy.
马铃薯(Solanum tuberosum L.)以木薯10号为研究对象,研究了外源赤霉素(gga3)、脱落酸(ABA)和低温胁迫对块茎激素含量、StTCP15基因表达模式和块茎休眠特性的影响。在GA 3处理和低温胁迫下,块茎休眠比对照组提前1周左右打破,但ABA处理对块茎休眠的促进作用不显著。液相色谱-质谱联用(LC-MS/ MS)测定结果表明,GA 3和低温胁迫处理的块茎中ABA含量低于对照组,而ABA处理的块茎中ABA含量高于对照组。甘油三酯处理后块茎中甘油三酯含量高于对照组,低温胁迫下甘油三酯含量低于对照组。休眠期间ABA含量持续增加,GA - 3含量波动,休眠打破时ABA含量迅速下降,GA - 3含量迅速上升,萌发期间ABA含量和GA - 3含量均增加。实时荧光定量PCR检测结果显示,各组在休眠期间StTCP15基因的表达量不断增加,休眠解除时StTCP15基因的表达量最高。StTCP15基因在低温胁迫第7天表达量增加约15倍,在室温下恢复。因此,StTCP15基因可以响应GA - 3、ABA和低温胁迫,并可能参与马铃薯块茎休眠的解除。
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引用次数: 1
Selection and validation of reference genes for RT-qPCR analysis in the pericarp of Litchi chinensis 荔枝果皮RT-qPCR内参基因的选择与验证
IF 1.5 4区 生物学 Q4 PLANT SCIENCES Pub Date : 2022-04-07 DOI: 10.32615/bp.2021.066
F. Li, J. Sun, J. L. Men, H. Li, G. Wang, S. Wang, J. Wang
GAGA-25 - GATA transcription factor 25; HDAC9 - histone deacetylase 9; HLM2B - histone-lysine_N-methyltransferase 2B; NtaA - N(alpha)-acetyltransferase 16, NatA auxiliary; pbP - peroxisome biogenesis protein 22-like; RFU1 - RING finger ubiquitin ligase; RT-qPCR reverse transcription qPCR; RUB1 - ubiquitin-NEDD8-like protein RUB1; STAM - Stam binding; TL-OEMC - translocon at the outer membrane of chloroplasts 64; UPF3 - UPF3 regulator of nonsense transcripts homolog UPF3; V - variation. Abstract Real-time reverse transcription quantitative PCR (RT-qPCR) is an important tool for gene expression analysis. Suitable reference genes are the basis of accurate and reliable RT-qPCR results. Litchi ( Litchi chinensis Sonn.) is a commercially important tropical and subtropical fruit, but rapid pericarp browning is a substantial negative impact on its commercial use. Reference gene validation could help in the screening for genes involved in the browning mechanism. We assessed 15 new candidate reference genes from litchi transcriptome to determine stable reference genes for RT-qPCR analysis of pericarps from different cultivars, with differing postharvest storage, and under pathogenic stress. Ct values, geNorm , Normfinder , and RefFinder algorithms, were used to identify genes with the most stable transcription. GAGA-25 was the gene with the most stable transcription for comparing different varieties of the fresh pericarp. HDAC9 was the gene with the most stable transcription for postharvest pericarp. STAM was the gene with the most stable transcription for inoculated pericarp. Of the candidate reference genes, GAGA-25 was the most stable reference gene across the complete sample set. This study evaluated reference gene stability for RT-qPCR in litchi pericarp. This work provides a foundation for using qPCR to study gene function and molecular mechanism studies of litchi pericarp browning.
GAGA-25 - GATA转录因子25;HDAC9 -组蛋白去乙酰化酶9;HLM2B -组蛋白赖氨酸n -甲基转移酶2B;NtaA - N(α)-乙酰转移酶16;pbP -过氧化物酶体生物发生蛋白22样;RFU1 -环指泛素连接酶;反转录qPCR;RUB1 -泛素- nedd8样蛋白RUB1;斯塔姆-斯塔姆装订;TL-OEMC -叶绿体外膜易位64;UPF3 -无义转录物同源物UPF3的UPF3调控因子;V -变异。实时反转录定量PCR (RT-qPCR)是基因表达分析的重要工具。合适的内参基因是RT-qPCR结果准确可靠的基础。荔枝(Litchi chinensis Sonn.)是一种重要的热带和亚热带商业水果,但果皮的快速褐变对其商业用途产生了重大的负面影响。参考基因验证有助于筛选参与褐变机制的基因。我们从荔枝转录组中筛选了15个新的候选内参基因,以确定不同品种、不同采后贮藏和不同致病胁迫下果皮RT-qPCR分析的稳定内参基因。Ct值、geNorm、Normfinder和RefFinder算法用于鉴定转录最稳定的基因。GAGA-25是比较不同品种新鲜果皮转录最稳定的基因。HDAC9是采后果皮转录最稳定的基因。STAM基因是接种果皮转录最稳定的基因。在候选内参基因中,GAGA-25是整个样本集中最稳定的内参基因。本研究对荔枝果皮RT-qPCR内参基因的稳定性进行了评价。本研究为利用qPCR技术研究荔枝果皮褐变的基因功能和分子机制奠定了基础。
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引用次数: 2
Novel Approaches for the Treatment of Patients with Richter's Syndrome. 治疗里希特综合征患者的新方法。
IF 3.8 4区 生物学 Q4 PLANT SCIENCES Pub Date : 2022-04-01 Epub Date: 2022-03-16 DOI: 10.1007/s11864-022-00973-1
Andrea Iannello, Silvia Deaglio, Tiziana Vaisitti

Opinion statement: In the last 10-15 years, the way to treat cancers has dramatically changed towards precision medicine approaches. These treatment options are mainly based on selective targeting against signaling pathways critical for or detrimentally activated in cancer cells in cancer cells, as well as exploiting molecules that are specifically expressed on neoplastic cells, also known as tumor-associated antigens. These considerations hold true also in the hematological field where a plethora of novel targeted agents have reached patients' bedside, significantly improving clinical responses. Chronic lymphocytic leukemia (CLL) is an example of how targeted therapies, such as BTK, PI3K, or Bcl-2 inhibitors as well as anti-CD20 antibodies, have improved patients' management, even when adopted as frontline treatment. However, these advancements do not apply to Richter's syndrome (RS), the transformation of CLL into a very aggressive and fatal lymphoma, occurring in 2-10% of patients. RS is usually a fast-growing lymphoma of the diffuse large B cell or the Hodgkin's variant, with a dismal prognosis. Despite advancements in depicting and understanding the genetic background of RS and its pathogenesis, no significant clinical results have been registered. In the last couple of years, several studies have started to investigate the impact of novel drugs or drug combinations and some of them have opened for clinical trials, currently in phase I or II, whose results will be soon available. This review will present an overview of current and most recent therapeutic options in RS, discussing also how results coming from xenograft models may help in designing and identifying novel treatment opportunities to overcome the lack of effective therapies.

意见陈述:在过去的 10-15 年间,治疗癌症的方式发生了巨大变化,逐渐转向精准医疗方法。这些治疗方案主要基于选择性靶向对癌细胞至关重要或在癌细胞中被有害激活的信号通路,以及利用肿瘤细胞上特异表达的分子(也称为肿瘤相关抗原)。这些考虑因素在血液病领域也同样适用,在该领域,大量新型靶向药物已进入患者床旁,显著改善了临床反应。慢性淋巴细胞白血病(CLL)就是一个例子,它的靶向疗法(如 BTK、PI3K 或 Bcl-2 抑制剂以及抗 CD20 抗体)改善了患者的治疗,甚至被作为一线治疗手段。然而,这些进展并不适用于里克特综合征(RS),里克特综合征是一种由 CLL 转化而来的侵袭性极强的致命淋巴瘤,发生率为 2-10%。RS通常是一种快速生长的弥漫大B细胞淋巴瘤或霍奇金变异型淋巴瘤,预后极差。尽管在描述和理解RS的遗传背景及其发病机制方面取得了进展,但临床结果并不显著。最近几年,一些研究开始调查新型药物或药物组合的影响,其中一些已经开始进行临床试验,目前正处于第一或第二阶段,很快就会有结果。本综述将概述目前和最新的 RS 治疗方案,并讨论异种移植模型的结果如何有助于设计和确定新的治疗机会,以克服缺乏有效疗法的问题。
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引用次数: 0
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Biologia Plantarum
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