Pub Date : 2024-07-18DOI: 10.14393/bj-v40n0a2024-67453
Heder Asdrubal Montañez Valencia, H. Amaral, Jordana Mayra Nassar, D. S. Andrade
The aim of this study was to evaluate the contribution of inoculation with microorganisms, mainly rhizobia, on pea (Pisum sativum) production using a systematic literature review approach and a comparative analysis of grain yield to provide information to fill gaps in nontraditional regions of cultivation for this legume. A systematic search strategy was implemented, targeting papers published in scientific journals accessible through the Web of Science® (WoS) database spanning from January 1990 to April 2022. The search terms "Pisum sativum," "inoculation," and "strain" were used. The differences between the pea grain yields from plants inoculated with nitrogen-fixing microorganisms and those from noninoculated plants (control) were analyzed using thirteen field-scale studies. Overall, pea inoculation/coinoculation promoted a greater grain yield (3118 ±210 kg ha-1) than noninoculation (2338± 127 kg ha-1), showing the importance of biological nitrogen fixation for legume crop production. In the pursuit of reducing pea production costs, emphasis has been placed on inoculation, highlighting the importance of comprehending the symbiotic relationship between peas and Rhizobium. Furthermore, this research aimed to explore the interaction between Rhizobium and selected microorganisms known to enhance plant growth to identify optimal combinations to boost pea productivity.
本研究的目的是通过系统的文献综述方法和谷物产量比较分析，评估微生物（主要是根瘤菌）接种对豌豆（Pisum sativum）产量的贡献，以提供信息填补该豆科植物非传统种植地区的空白。我们采用了系统的搜索策略，目标是 1990 年 1 月至 2022 年 4 月期间可通过 Web of Science® (WoS) 数据库访问的科学期刊上发表的论文。搜索关键词为 "Pisum sativum"、"接种 "和 "菌株"。利用 13 项田间规模的研究分析了接种固氮微生物的植株与未接种植株（对照）的豌豆籽粒产量之间的差异。总体而言，豌豆接种/联合接种比不接种（2338± 127 kg ha-1）促进了更高的谷物产量（3118±210 kg ha-1），显示了生物固氮对豆科作物生产的重要性。为了降低豌豆的生产成本，重点放在了接种上，这凸显了理解豌豆与根瘤菌之间共生关系的重要性。此外，这项研究旨在探索根瘤菌与已知能促进植物生长的特定微生物之间的相互作用，以确定提高豌豆产量的最佳组合。
{"title":"Rhizobia inoculation increases pea grain yield: an overview and challenges","authors":"Heder Asdrubal Montañez Valencia, H. Amaral, Jordana Mayra Nassar, D. S. Andrade","doi":"10.14393/bj-v40n0a2024-67453","DOIUrl":"https://doi.org/10.14393/bj-v40n0a2024-67453","url":null,"abstract":"The aim of this study was to evaluate the contribution of inoculation with microorganisms, mainly rhizobia, on pea (Pisum sativum) production using a systematic literature review approach and a comparative analysis of grain yield to provide information to fill gaps in nontraditional regions of cultivation for this legume. A systematic search strategy was implemented, targeting papers published in scientific journals accessible through the Web of Science® (WoS) database spanning from January 1990 to April 2022. The search terms \"Pisum sativum,\" \"inoculation,\" and \"strain\" were used. The differences between the pea grain yields from plants inoculated with nitrogen-fixing microorganisms and those from noninoculated plants (control) were analyzed using thirteen field-scale studies. Overall, pea inoculation/coinoculation promoted a greater grain yield (3118 ±210 kg ha-1) than noninoculation (2338± 127 kg ha-1), showing the importance of biological nitrogen fixation for legume crop production. In the pursuit of reducing pea production costs, emphasis has been placed on inoculation, highlighting the importance of comprehending the symbiotic relationship between peas and Rhizobium. Furthermore, this research aimed to explore the interaction between Rhizobium and selected microorganisms known to enhance plant growth to identify optimal combinations to boost pea productivity.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141823961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Burdock (Arctium lappa L.), which belongs to the Compositae family, possesses significant medicinal and nutritional value. Burdock root is abundant in proteins and essential amino acids. In this study, proteins from burdock roots were extracted by alkali extraction and acid sedimentation, and the optimal conditions for protein extraction from burdock roots and protein characteristics were explored. The optimal conditions for protein extraction with a yield rate of 9.03% were 40°C extraction temperature, pH 8.0, a solid–liquid ratio of 1:25 (g/mL), and extraction time of 65 minutes. Single-factor and orthogonal tests on the extraction rate of burdock proteins under different conditions found that the extraction rate of protein from burdock roots were influenced by pH, extraction temperature, solid–liquid ratio, and extraction time, in this order. The solubility and gelation properties of burdock protein were similar to those of soybean protein; however, burdock protein demonstrated 1.2 times the oil absorption capacity and half the water retention capacity of soybean protein. Analysis of the amino acid contents of burdock protein revealed that burdock root contains significant essential and non-essential amino acids, with valine content being the highest.
{"title":"Study on extraction conditions and characteristics of protein from burdock roots","authors":"XiuMei Gong, Fei Chang, Ximin Yao, Peng Leng, Yuqing Miao, GuiZhi Zhang","doi":"10.14393/bj-v40n0a2024-70783","DOIUrl":"https://doi.org/10.14393/bj-v40n0a2024-70783","url":null,"abstract":"Burdock (Arctium lappa L.), which belongs to the Compositae family, possesses significant medicinal and nutritional value. Burdock root is abundant in proteins and essential amino acids. In this study, proteins from burdock roots were extracted by alkali extraction and acid sedimentation, and the optimal conditions for protein extraction from burdock roots and protein characteristics were explored. The optimal conditions for protein extraction with a yield rate of 9.03% were 40°C extraction temperature, pH 8.0, a solid–liquid ratio of 1:25 (g/mL), and extraction time of 65 minutes. Single-factor and orthogonal tests on the extraction rate of burdock proteins under different conditions found that the extraction rate of protein from burdock roots were influenced by pH, extraction temperature, solid–liquid ratio, and extraction time, in this order. The solubility and gelation properties of burdock protein were similar to those of soybean protein; however, burdock protein demonstrated 1.2 times the oil absorption capacity and half the water retention capacity of soybean protein. Analysis of the amino acid contents of burdock protein revealed that burdock root contains significant essential and non-essential amino acids, with valine content being the highest.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141828295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-17DOI: 10.14393/bj-v40n0a2024-66942
Lais Renata Almeida Cezário, G. Ambrosano, G. Ambrosano, Anderson Taíra, R. Possobon, M. C. Meneghim, K. L. Cortellazzi
This cross-sectional study aimed to assess whether levels of anxiety, perceived stress, and self-perception of happiness during the Covid-19 pandemic were lower among Tai Chi (TC) practitioners. An online questionnaire was applied from September 2020 to November 2021 through email list and instant messaging application "WhatsApp". In total 368 individuals, 341 of whom did not practice TC, coming from a university and sports academies and 27 TC practitioners from the Brazilian Society of TC and Oriental Culture were included. Sociodemographic, mental health, habits related to physical activity and the State Anxiety Inventory (STAI), Perceived Stress Scale (PSS14) and the Andrews Faces Scale were collected to assess level of state-anxiety, perceived-stress, and self-perception of happiness, respectively. Each independent variable with the outcome variables (severe anxiety level, severe stress level, moderate/severe stress level and self-perception of happiness) were analyzed for simple and multiple logistic regression model. Individuals who do not practice TC are more likely to have a severe level of anxiety (OR=3.55; CI95%CI: 1.37-9.20) and a moderate/severe level of stress (OR=2.60; 95%CI: 1.07-6.35), p<0.05. TC practitioners are more likely to perceive themselves as happy than non-practitioners (OR=3.18; 95%CI: 1.16-8.71), p<0.05. Do not practice regular physical activity (OR=2.88; 95%CI:1.50-4.34); (OR=1.7; 95%CI: 95%: 1.09-2.74) and being young were associated with high levels of stress, p<0.05. The TC practice during the pandemic promoted lower level of perceived stress, state anxiety and greater self-perception of happiness, showing the importance of TC practice and maintaining the frequency of physical activity during the pandemic.
{"title":"Effect of Tai Chi on adult mental health during the pandemic and factors associated: a cross-sectional study","authors":"Lais Renata Almeida Cezário, G. Ambrosano, G. Ambrosano, Anderson Taíra, R. Possobon, M. C. Meneghim, K. L. Cortellazzi","doi":"10.14393/bj-v40n0a2024-66942","DOIUrl":"https://doi.org/10.14393/bj-v40n0a2024-66942","url":null,"abstract":"This cross-sectional study aimed to assess whether levels of anxiety, perceived stress, and self-perception of happiness during the Covid-19 pandemic were lower among Tai Chi (TC) practitioners. An online questionnaire was applied from September 2020 to November 2021 through email list and instant messaging application \"WhatsApp\". In total 368 individuals, 341 of whom did not practice TC, coming from a university and sports academies and 27 TC practitioners from the Brazilian Society of TC and Oriental Culture were included. Sociodemographic, mental health, habits related to physical activity and the State Anxiety Inventory (STAI), Perceived Stress Scale (PSS14) and the Andrews Faces Scale were collected to assess level of state-anxiety, perceived-stress, and self-perception of happiness, respectively. Each independent variable with the outcome variables (severe anxiety level, severe stress level, moderate/severe stress level and self-perception of happiness) were analyzed for simple and multiple logistic regression model. Individuals who do not practice TC are more likely to have a severe level of anxiety (OR=3.55; CI95%CI: 1.37-9.20) and a moderate/severe level of stress (OR=2.60; 95%CI: 1.07-6.35), p<0.05. TC practitioners are more likely to perceive themselves as happy than non-practitioners (OR=3.18; 95%CI: 1.16-8.71), p<0.05. Do not practice regular physical activity (OR=2.88; 95%CI:1.50-4.34); (OR=1.7; 95%CI: 95%: 1.09-2.74) and being young were associated with high levels of stress, p<0.05. The TC practice during the pandemic promoted lower level of perceived stress, state anxiety and greater self-perception of happiness, showing the importance of TC practice and maintaining the frequency of physical activity during the pandemic.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141828718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-17DOI: 10.14393/bj-v40n0a2024-69905
Cemal Yazir, N. Sultanoglu, M. Guvenir, Ulas Hurdoganoglu, H. Yavuz, K. Suer
Fitness centers offer an opportunity for investigating the indirect transmission of pathogens. Many people with varying levels of personal hygiene share sports equipment where direct surface-to-skin contact occurs. This study aimed to investigate the bacterial load and fungal and methicillin-resistant Staphylococcus aureus (MRSA) contamination on predetermined sports equipment in fitness centers in Northern Cyprus. Additionally, volunteer personal trainers and gym members were screened to detect whether they were carriers of MRSA. Samples were collected from six fitness centers on sports equipment. MRSA carrier status was investigated for personal trainers (n=10) and gym members (n=100). The study used culture-dependent techniques and used SPSS 20 software for statistical analysis. There was no statistically significant difference between fungal growth on the sports equipment, and Aspergillus spp. were predominant. However, one of the fitness centers had a statistically significant difference in fungal growth compared to the others (p<0.005). There was no significant difference in the bacterial load among the sports equipment, but there was a significant difference among the fitness centers (p<0.009). Among all tested individuals, only 2.7% (3/110) were MRSA-positive, all of whom were gym members. No MRSA was detected on any of the equipment. Despite the increasing incidence of community-acquired MRSA infections, the fitness centers in this study did not appear to be significant sources of staphylococcal or fungal infections. However, the detection of MRSA carriers among gym members suggests that the spread of MRSA between individuals in gyms is still possible.
{"title":"Infectious disease agent surveillance in fitness centers in northern cyprus: is methicillin-resistant Staphylococcus aureus (mrsa) infection a threat?","authors":"Cemal Yazir, N. Sultanoglu, M. Guvenir, Ulas Hurdoganoglu, H. Yavuz, K. Suer","doi":"10.14393/bj-v40n0a2024-69905","DOIUrl":"https://doi.org/10.14393/bj-v40n0a2024-69905","url":null,"abstract":"Fitness centers offer an opportunity for investigating the indirect transmission of pathogens. Many people with varying levels of personal hygiene share sports equipment where direct surface-to-skin contact occurs. This study aimed to investigate the bacterial load and fungal and methicillin-resistant Staphylococcus aureus (MRSA) contamination on predetermined sports equipment in fitness centers in Northern Cyprus. Additionally, volunteer personal trainers and gym members were screened to detect whether they were carriers of MRSA. Samples were collected from six fitness centers on sports equipment. MRSA carrier status was investigated for personal trainers (n=10) and gym members (n=100). The study used culture-dependent techniques and used SPSS 20 software for statistical analysis. There was no statistically significant difference between fungal growth on the sports equipment, and Aspergillus spp. were predominant. However, one of the fitness centers had a statistically significant difference in fungal growth compared to the others (p<0.005). There was no significant difference in the bacterial load among the sports equipment, but there was a significant difference among the fitness centers (p<0.009). Among all tested individuals, only 2.7% (3/110) were MRSA-positive, all of whom were gym members. No MRSA was detected on any of the equipment. Despite the increasing incidence of community-acquired MRSA infections, the fitness centers in this study did not appear to be significant sources of staphylococcal or fungal infections. However, the detection of MRSA carriers among gym members suggests that the spread of MRSA between individuals in gyms is still possible.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141828271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-17DOI: 10.14393/bj-v40n0a2024-71135
Xiaoguang Chen, Q. Lv, Haonan Li, Zhe Wang
It has been reported that dodecanoic acid (DDA) exerts anticancer effects on cancers of the reproductive system and digestive system. However, its role in liver cancer and its potential mechanism have rarely been defined. Therefore, in this study, Hepa 1-6 liver cancer cells were incubated with different DDA concentrations (0.1, 0.3, 0.5, 1, 2, 4 mM) for 24, 48 and 72h, and the optimal DDA concentration was determined via a cell viability test. Apoptosis and cell cycle distribution were determined by flow cytometry. SOD activity, mitochondrial membrane potential (MMP), ATP, GSH and ROS levels were measured by commercial assay kits; Bcl-2, Bax and Caspase-3 protein levels were analyzed by western blot. The results showed that 0.5 mM DDA decreased cell viability in a time-dependent manner, so this concentration was used to investigate how DDA leads to Hepa 1-6 cell apoptosis. After treatment with DDA, a significant, time-dependent increase in the cell apoptotic rate was detected despite the accumulation of S-phase cells. The increased ROS levels and decreased GSH levels and SOD activity in DDA-treated cells indicated the occurrence of oxidative stress. Mitochondrial dysfunction was evidenced by a decreased MMP and reduced ATP levels. Cell apoptotic death via the mitochondrial pathway was indicated by a reduced Bcl-2/Bax ratio and increased caspase-3 protein levels. It can be concluded that DDA can effectively trigger liver cancer cell death by inducing oxidative stress and disrupting mitochondrial function. These findings provide new insight into the potential mechanism of action of DDA in liver cancer.
{"title":"Dodecanoic acid induces oxidative stress-mediated death in liver cancer cells through the mitochondrial pathway","authors":"Xiaoguang Chen, Q. Lv, Haonan Li, Zhe Wang","doi":"10.14393/bj-v40n0a2024-71135","DOIUrl":"https://doi.org/10.14393/bj-v40n0a2024-71135","url":null,"abstract":"It has been reported that dodecanoic acid (DDA) exerts anticancer effects on cancers of the reproductive system and digestive system. However, its role in liver cancer and its potential mechanism have rarely been defined. Therefore, in this study, Hepa 1-6 liver cancer cells were incubated with different DDA concentrations (0.1, 0.3, 0.5, 1, 2, 4 mM) for 24, 48 and 72h, and the optimal DDA concentration was determined via a cell viability test. Apoptosis and cell cycle distribution were determined by flow cytometry. SOD activity, mitochondrial membrane potential (MMP), ATP, GSH and ROS levels were measured by commercial assay kits; Bcl-2, Bax and Caspase-3 protein levels were analyzed by western blot. The results showed that 0.5 mM DDA decreased cell viability in a time-dependent manner, so this concentration was used to investigate how DDA leads to Hepa 1-6 cell apoptosis. After treatment with DDA, a significant, time-dependent increase in the cell apoptotic rate was detected despite the accumulation of S-phase cells. The increased ROS levels and decreased GSH levels and SOD activity in DDA-treated cells indicated the occurrence of oxidative stress. Mitochondrial dysfunction was evidenced by a decreased MMP and reduced ATP levels. Cell apoptotic death via the mitochondrial pathway was indicated by a reduced Bcl-2/Bax ratio and increased caspase-3 protein levels. It can be concluded that DDA can effectively trigger liver cancer cell death by inducing oxidative stress and disrupting mitochondrial function. These findings provide new insight into the potential mechanism of action of DDA in liver cancer.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141829645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-17DOI: 10.14393/bj-v40n0a2024-70492
D. R. C. Cruz, Natasha Ohanny da Costa Monteiro, Izabely Vitória Lucas Ferreira, Victórya Gabrielly Manzan Souza, Juracy Barroso Neto, M. A. Silva, A. Nascente
Multifunctional phosphate solubilizing microorganisms can contribute to reducing phosphorus doses without affecting the grain yield of crops. The aim of this work was to evaluate agricultural production systems involving soybean-maize and soybean-rice successions, inoculation of beneficial microorganisms and application of phosphorus doses with a view to sustainable intensification of agriculture and soil health and fertility in the Cerrados region. The experimental design was a randomized block design in a 2x4 factorial scheme with four replications. The treatments were composed of the combination of two phosphorus doses, 50% (45 kg ha-1 of P2O5) and 100% (90 kg ha-1 of P2O5) of the recommended dose with four uses of multifunctional microorganisms: 1. BRM 32111 (Burkholderia sp.), 2. BRM 32114 (Serratia marcescens), 3. co-inoculation (BRM 32111 + BRM 32114), and 4. control (no application of microorganisms). The microorganisms provided significant increases in the 100-grain weight and grain yield of soybeans, dry matter and nutrient accumulation of rice and maize, reduction of phytopathogenic fungus propagules, and increased accumulation of nutrients and activity of the enzymes Betaglicosidase and Arilsulfatase in the soil. Applying 50% phosphorus reduced the 100-grain weight and grain yield of soybean, dry matter and nutrient accumulation of rice, propagules of Trichoderma spp., and the nutrients in the soil. The soybean-maize succession showed higher levels of Arylsulfatase than the soybean-rice succession. The highest soybean yields were obtained by applying BRM 32114 with 50% and co-inoculation with 100% phosphorus.
{"title":"Multifunctional microorganisms and phosphorus dosages in soybean-maize and soybean-rice successions under no-till systems in the cerrado","authors":"D. R. C. Cruz, Natasha Ohanny da Costa Monteiro, Izabely Vitória Lucas Ferreira, Victórya Gabrielly Manzan Souza, Juracy Barroso Neto, M. A. Silva, A. Nascente","doi":"10.14393/bj-v40n0a2024-70492","DOIUrl":"https://doi.org/10.14393/bj-v40n0a2024-70492","url":null,"abstract":"Multifunctional phosphate solubilizing microorganisms can contribute to reducing phosphorus doses without affecting the grain yield of crops. The aim of this work was to evaluate agricultural production systems involving soybean-maize and soybean-rice successions, inoculation of beneficial microorganisms and application of phosphorus doses with a view to sustainable intensification of agriculture and soil health and fertility in the Cerrados region. The experimental design was a randomized block design in a 2x4 factorial scheme with four replications. The treatments were composed of the combination of two phosphorus doses, 50% (45 kg ha-1 of P2O5) and 100% (90 kg ha-1 of P2O5) of the recommended dose with four uses of multifunctional microorganisms: 1. BRM 32111 (Burkholderia sp.), 2. BRM 32114 (Serratia marcescens), 3. co-inoculation (BRM 32111 + BRM 32114), and 4. control (no application of microorganisms). The microorganisms provided significant increases in the 100-grain weight and grain yield of soybeans, dry matter and nutrient accumulation of rice and maize, reduction of phytopathogenic fungus propagules, and increased accumulation of nutrients and activity of the enzymes Betaglicosidase and Arilsulfatase in the soil. Applying 50% phosphorus reduced the 100-grain weight and grain yield of soybean, dry matter and nutrient accumulation of rice, propagules of Trichoderma spp., and the nutrients in the soil. The soybean-maize succession showed higher levels of Arylsulfatase than the soybean-rice succession. The highest soybean yields were obtained by applying BRM 32114 with 50% and co-inoculation with 100% phosphorus.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141830502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-17DOI: 10.14393/bj-v40n0a2024-72612
Gabriela Mayara Oliveira Nascimento, Wender Batista de Souza, G. Camargos, M. Munhoz, Eduardo Henrique da Silva, A. Paleari
Bilateral Balanced Occlusion (BBO) and Canine Guidance (CG) are occlusal schemes that have been successfully used in complete dentures (CDs). The objective of this study was to evaluate the effects of CG compared to BBO on satisfaction, oral health-related quality of life (OHRQoL) and masticatory performance in CDs wearers with severe resorbed alveolar ridge by means of a crossover trial. Sixteen edentulous patients with severe resorbed ridges were treated with new maxillary and mandibular CDs. After adaptation period, they were randomly divided into two groups according to a sequence of occlusal schemes: BBO for 30 days followed by CG for 30 days or, CG for 30 days followed by BBO for 30 days. Patient satisfaction with their dentures, OHRQoL and masticatory performance were assessed immediately 30 days after of using each occlusal scheme. The results of satisfaction and quality of life were using Wilcoxon test, and data regarding masticatory performance were analyzed through paired t-test (α = .05). The occlusal scheme used in complete dentures had no significant influence on general patient satisfaction (p = 0.06), overall quality of life (p = 0.09), or masticatory performance of patients (p = 0.182). Both BBO and CG can be considered as suitable for oral rehabilitation with CDs in patients with severe resorbed alveolar ridges. However, CG may be considered as a scheme of choice by clinicians due to its greater simplicity and shorter execution time.
{"title":"Impact of occlusal schemes on prognosis of conventional complete dentures in patients with severely resorbed alveolar ridges","authors":"Gabriela Mayara Oliveira Nascimento, Wender Batista de Souza, G. Camargos, M. Munhoz, Eduardo Henrique da Silva, A. Paleari","doi":"10.14393/bj-v40n0a2024-72612","DOIUrl":"https://doi.org/10.14393/bj-v40n0a2024-72612","url":null,"abstract":"Bilateral Balanced Occlusion (BBO) and Canine Guidance (CG) are occlusal schemes that have been successfully used in complete dentures (CDs). The objective of this study was to evaluate the effects of CG compared to BBO on satisfaction, oral health-related quality of life (OHRQoL) and masticatory performance in CDs wearers with severe resorbed alveolar ridge by means of a crossover trial. Sixteen edentulous patients with severe resorbed ridges were treated with new maxillary and mandibular CDs. After adaptation period, they were randomly divided into two groups according to a sequence of occlusal schemes: BBO for 30 days followed by CG for 30 days or, CG for 30 days followed by BBO for 30 days. Patient satisfaction with their dentures, OHRQoL and masticatory performance were assessed immediately 30 days after of using each occlusal scheme. The results of satisfaction and quality of life were using Wilcoxon test, and data regarding masticatory performance were analyzed through paired t-test (α = .05). The occlusal scheme used in complete dentures had no significant influence on general patient satisfaction (p = 0.06), overall quality of life (p = 0.09), or masticatory performance of patients (p = 0.182). Both BBO and CG can be considered as suitable for oral rehabilitation with CDs in patients with severe resorbed alveolar ridges. However, CG may be considered as a scheme of choice by clinicians due to its greater simplicity and shorter execution time.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141830550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-17DOI: 10.14393/bj-v40n0a2024-67413
Luiza Monteiro Böer, Isabela Cinquini Junqueira, Thais Cardoso do Nascimento, A. Guilarde, V. Feres, K. C. Alcântara
The literature describes monocyte-lymphocyte (MLR), neutrophil-lymphocyte (NLR), and platelet-lymphocyte (PLR) ratios as prognostic biomarkers. However, in the case of dengue infection, patient clinical management and nonspecific laboratory tests determine the prognosis. Therefore, this study analyzed MLR, NLR, and PLR as prognostic biomarkers of dengue infection. Our study was based on a clinical cohort of dengue patients in Brazil between 2012 and 2013. From 193 patients, 164 (85.0%) were classified as dengue fever (DF), 19 (9.8%) as dengue hemorrhagic fever (DHF), and 10 (5.2%) as intermediate DF/DHF. DHF cases were significantly associated with MLR > 0.13 (OR: 5.72, 95% CI: 1.28-25.60, p < 0.05) and PLR ≤ 80.68 (OR: 4.26; 95% CI 1.60-11.33; p < 0.05). Our results suggest that MLR increase, and PLR decrease indicate a higher likelihood of worsening the clinical status.
{"title":"Monocyte-lymphocyte, neutrophil-lymphocyte, and platelet-lymphocyte ratios as inflammatory biomarkers of clinical dengue severity","authors":"Luiza Monteiro Böer, Isabela Cinquini Junqueira, Thais Cardoso do Nascimento, A. Guilarde, V. Feres, K. C. Alcântara","doi":"10.14393/bj-v40n0a2024-67413","DOIUrl":"https://doi.org/10.14393/bj-v40n0a2024-67413","url":null,"abstract":"The literature describes monocyte-lymphocyte (MLR), neutrophil-lymphocyte (NLR), and platelet-lymphocyte (PLR) ratios as prognostic biomarkers. However, in the case of dengue infection, patient clinical management and nonspecific laboratory tests determine the prognosis. Therefore, this study analyzed MLR, NLR, and PLR as prognostic biomarkers of dengue infection. Our study was based on a clinical cohort of dengue patients in Brazil between 2012 and 2013. From 193 patients, 164 (85.0%) were classified as dengue fever (DF), 19 (9.8%) as dengue hemorrhagic fever (DHF), and 10 (5.2%) as intermediate DF/DHF. DHF cases were significantly associated with MLR > 0.13 (OR: 5.72, 95% CI: 1.28-25.60, p < 0.05) and PLR ≤ 80.68 (OR: 4.26; 95% CI 1.60-11.33; p < 0.05). Our results suggest that MLR increase, and PLR decrease indicate a higher likelihood of worsening the clinical status.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141828387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-17DOI: 10.14393/bj-v40n0a2024-71926
Dandara Maria Clara do Rosario Barbosa, F. Rocha, C. Costa, W. M. Nascimento, Edson Hydu Mizobutsi, H. C. R. Catão
This research investigated the reaction of chickpea genotypes to Fusarium oxysporum CML 2878, seed health quality, and seed treatment with Carbendazim + Thiram fungicide. The roots of 15-day-old chickpea seedlings were injured, immersed in fungal suspension (4.5 x 107 conidia/mL), and transplanted. Thirty days later, F. oxysporum incidence in the genotypes, pathogenicity, and fresh mass and length of the root and shoot were evaluated. The conducted seed health test followed the incubation methodology on a paper substrate. The seed health and sand emergence tests used a factorial arrangement with two seed treatments (treated with fungicide and untreated) and nine genotypes. Total emergence and the number of normal and abnormal plants were evaluated. All genotypes were susceptible to F. oxysporum CML 2878, thus yellowing and browning the leaves and reducing root development. The primary fungi associated with the seeds of the studied genotypes were Aspergillus sp., Fusarium sp., Rhizopus sp., and Penicillium sp. The fungicide was highly efficient in fungal control but reduced emergence, weight, seedling height, and the number of normal seedlings in the genotypes. This study demonstrated that the evaluated genotypes were susceptible to F. oxysporum CML 2878 and Fusarium sp., spread by seeds. Although fungicidal control was efficient for Fusarium, it may interfere with chickpea germination and vigor.
本研究调查了鹰嘴豆基因型对 Fusarium oxysporum CML 2878 的反应、种子健康质量以及使用多菌灵 + Thiram 杀菌剂处理种子的情况。将 15 天大的鹰嘴豆幼苗的根部弄伤，浸入真菌悬浮液（4.5 x 107 分生孢子/毫升）中，然后移植。30 天后，对不同基因型的 F. oxysporum 发生率、致病性、根和芽的新鲜质量和长度进行评估。种子健康试验采用的是在纸基质上培养的方法。种子健康和沙土萌发试验采用因子排列法，有两种种子处理方法（用杀真菌剂处理和未处理）和九种基因型。对总出苗率以及正常和异常植株的数量进行了评估。所有基因型都对 F. oxysporum CML 2878 易感，因此叶片变黄、变褐，根系发育减弱。与所研究基因型种子相关的主要真菌有曲霉菌属、镰刀菌属、根霉菌属和青霉菌属。杀菌剂对真菌的控制效率很高，但会降低基因型的出苗率、重量、苗高和正常苗数。这项研究表明，所评估的基因型对通过种子传播的 F. oxysporum CML 2878 和 Fusarium sp.虽然杀真菌剂对镰刀菌有效，但可能会影响鹰嘴豆的发芽和活力。
{"title":"Genotypic reaction of chickpea to Fusarium oxysporum and seed sanitary quality","authors":"Dandara Maria Clara do Rosario Barbosa, F. Rocha, C. Costa, W. M. Nascimento, Edson Hydu Mizobutsi, H. C. R. Catão","doi":"10.14393/bj-v40n0a2024-71926","DOIUrl":"https://doi.org/10.14393/bj-v40n0a2024-71926","url":null,"abstract":"This research investigated the reaction of chickpea genotypes to Fusarium oxysporum CML 2878, seed health quality, and seed treatment with Carbendazim + Thiram fungicide. The roots of 15-day-old chickpea seedlings were injured, immersed in fungal suspension (4.5 x 107 conidia/mL), and transplanted. Thirty days later, F. oxysporum incidence in the genotypes, pathogenicity, and fresh mass and length of the root and shoot were evaluated. The conducted seed health test followed the incubation methodology on a paper substrate. The seed health and sand emergence tests used a factorial arrangement with two seed treatments (treated with fungicide and untreated) and nine genotypes. Total emergence and the number of normal and abnormal plants were evaluated. All genotypes were susceptible to F. oxysporum CML 2878, thus yellowing and browning the leaves and reducing root development. The primary fungi associated with the seeds of the studied genotypes were Aspergillus sp., Fusarium sp., Rhizopus sp., and Penicillium sp. The fungicide was highly efficient in fungal control but reduced emergence, weight, seedling height, and the number of normal seedlings in the genotypes. This study demonstrated that the evaluated genotypes were susceptible to F. oxysporum CML 2878 and Fusarium sp., spread by seeds. Although fungicidal control was efficient for Fusarium, it may interfere with chickpea germination and vigor.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141831247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-03DOI: 10.14393/bj-v40n0a2024-70910
Ebru Beyzi, M. Külahcı, Nebahat Aytuna Çerçi
Bikaverin is a reddish pigment produced by different fungi species (Mycogone jaapii, Verticillium agaricinum, Beauveria bassiana, Paecilomyces fumosoroseus, Polyporus sulphureus), mainly of the Fusarium genus. Due to its pigment feature, bikaverin can be used as a dye in various fields in the industry. However, it is extremely important to study the mutagenic/genotoxic effects, cytotoxic effects and antimicrobial properties of bikaverin for application of industrial areas. In the study, the mutagenic, cytotoxic and antimicrobial effects of bikaverin were investigated. The mutagenic effect of bikaverin was studied with the Ames test. Salmonella typhimurium TA97a, TA98, TA100, TA102 and TA1535 strains were used in the test. Five different doses of bikaverin (0.075, 0.1, 0.2, 0.3 and 0.5 μg/plate) were tested against strains. It was determined that there was no mutagenic effect of bikaverin. The cytotoxicity of bikaverin was evaluated by MTT test on L929 fibroblast cell line. Bikaverin demonstrated no cytotoxic effect on L929 fibroblast cell line, according to cell viability calculations that showed >73% for all concentrations (1, 0.5, 0.4, 0.3, 0.2, 0.1, 0.075, 0.05, 0.025, 0.01, 0.005 and 0.001 μg/mL) examined. Bikaverin's IC50 value was determined to be 1.79±0.51 g/mL. The antimicrobial activity of the bikaverin was evaluated by using the microdilution method. Bikaverin was found to have antimicrobial effects on Methicillin resistant Staphylococcus aureus, Vancomycin resistant Enterococcus faecalis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Candida albicans and Candida krusei, as MIC values ranged from 1.25 -5 µg/ mL.
{"title":"Investigation of the mutagenic, cytotoxic and antimicrobial effect of bikaverin mycotoxin","authors":"Ebru Beyzi, M. Külahcı, Nebahat Aytuna Çerçi","doi":"10.14393/bj-v40n0a2024-70910","DOIUrl":"https://doi.org/10.14393/bj-v40n0a2024-70910","url":null,"abstract":"Bikaverin is a reddish pigment produced by different fungi species (Mycogone jaapii, Verticillium agaricinum, Beauveria bassiana, Paecilomyces fumosoroseus, Polyporus sulphureus), mainly of the Fusarium genus. Due to its pigment feature, bikaverin can be used as a dye in various fields in the industry. However, it is extremely important to study the mutagenic/genotoxic effects, cytotoxic effects and antimicrobial properties of bikaverin for application of industrial areas. In the study, the mutagenic, cytotoxic and antimicrobial effects of bikaverin were investigated. The mutagenic effect of bikaverin was studied with the Ames test. Salmonella typhimurium TA97a, TA98, TA100, TA102 and TA1535 strains were used in the test. Five different doses of bikaverin (0.075, 0.1, 0.2, 0.3 and 0.5 μg/plate) were tested against strains. It was determined that there was no mutagenic effect of bikaverin. The cytotoxicity of bikaverin was evaluated by MTT test on L929 fibroblast cell line. Bikaverin demonstrated no cytotoxic effect on L929 fibroblast cell line, according to cell viability calculations that showed >73% for all concentrations (1, 0.5, 0.4, 0.3, 0.2, 0.1, 0.075, 0.05, 0.025, 0.01, 0.005 and 0.001 μg/mL) examined. Bikaverin's IC50 value was determined to be 1.79±0.51 g/mL. The antimicrobial activity of the bikaverin was evaluated by using the microdilution method. Bikaverin was found to have antimicrobial effects on Methicillin resistant Staphylococcus aureus, Vancomycin resistant Enterococcus faecalis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Candida albicans and Candida krusei, as MIC values ranged from 1.25 -5 µg/ mL.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140747379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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