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Potential of rust-resistant arabica coffee cultivars for specialty coffee production 抗锈蚀阿拉比卡咖啡品种在特种咖啡生产中的潜力
IF 0.5 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-03-31 DOI: 10.14393/bj-v39n0a2023-66103
Tharyn Reichel, M. L. V. Resende, D. H. S. Nadaleti, Fábio de Oliveira Santos, C. E. Botelho
Rust is the main disease affecting Coffea arabica, the most economically important coffee species. The objective of this study was to analyze C. arabica cultivars with different levels of rust resistance, including bean size, raw bean appearance, final sensory scores (FSS), and aroma and taste nuances of the coffee cup. The experiment was designed in randomized blocks (RBD) with three replications and 20 treatments (cultivars), totaling 60 experimental plots. The rust-susceptible cultivars IPR 100, Rubi MG 1192, and Topázio MG 1190 were compared with 17 rust-resistant cultivars. Cultivars IPR 103, MGS Aranãs, and Saíra II presented the highest percentages of high sieves, highest scores of raw bean appearance, and low percentages of mocha-type beans. All cultivars had FSS above 82 and were classified as specialty coffees. The cultivars with the highest FSS (Arara and Catiguá MG2) showed a greater diversity of coffee cup aroma and flavor nuances. Rust-resistant Arabica coffee cultivars are promising for the physical quality of beans and have potential for the specialty coffee market.
铁锈是影响咖啡的主要疾病,咖啡是最重要的经济品种。本研究的目的是分析具有不同防锈水平的阿拉比卡品种,包括咖啡豆大小、生豆外观、最终感官评分(FSS)以及咖啡杯的香气和味道细微差别。本试验采用随机区组(RBD)设计,共有60个试验区,共有3个重复和20个处理(品种)。将易感锈品种IPR 100、Rubi MG 1192和Topázio MG 1190与17个抗病品种进行了比较。品种IPR 103、MGS Aranãs和Saíra II表现出最高的高筛百分比、最高的生豆外观分数和较低的摩卡类型豆百分比。所有品种的FSS均在82以上,属于特种咖啡品种。FSS最高的品种(Arara和CatiguáMG2)表现出更大的咖啡杯香气和风味细微差别的多样性。抗锈阿拉比卡咖啡品种在咖啡豆的物理质量方面很有前景,并有潜力进入特种咖啡市场。
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引用次数: 0
Productivity and mortality, of Fusarium oxysporum f. sp. passiflorae in passionfruit vines brs rubi do cerrado, grafted onto different rootstocks 将尖孢镰刀菌(Fusarium oxysporum f.sp.passiflorae)嫁接到不同砧木上的百香果藤(brs rubi do cerrado)的生产力和死亡率
IF 0.5 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-03-31 DOI: 10.14393/bj-v39n0a2023-61288
Luciano Gomes Ferreira, F. C. Juliatti, M. F. Moraes, L. Kobayasti, G. Roncatto, B. Juliatti, Risely Ferraz de Almeida, Carlos Antônio Távora Araújo
The use of scions grafted onto passionfruit vines has become an alternative to their cultivation in areas with a history of fusariosis. However, the combinations between the graft and rootstock can influence on the productivity and longevity of the passionfruit farm. The objective was to evaluate the productivity and mortality of the passionfruit cv BRS Rubi do Cerrado grafted onto three species of rootstock in Terra Nova do Norte-MT. The design was a randomized block with 4 treatments BRS Rubi do Cerrado (BRS-RC) seedling plant along with those grafted onto three species of rootstock: Passiflora gibertii, Passiflora alata; Passiflora nitida. The scions were grafted through a cleft graft and the planting performed after 70 days. The evaluation was made of the total number and weight of the fruits, along with productivity and mortality of plants. Results showed that the BRS-RC, grafted onto P. gibertii and P. nitida, presented the best performance for weight of fruits, number of fruits and productivity. The cultivar that was grafted onto different rootstocks presented higher productivity in regards to the seedling plant. The BRS-RC, grafted onto P. nitida, presented a zero-mortality rate over the 16.5 months of cultivation. The rootstocks P. nitida and P. gibertii were superior to those of P. alata in reducing mortality in the passionfruit vine by Fusarium oxysporum f. sp. passiflorae. The confirmation was made of the pathogen Fusarium oxysporum f. passiflorae isolated in the experimental area in scions of the BRS-RC inoculated at 70 days of age.
接穗嫁接到西番莲葡萄藤上的使用已成为在有镰刀病历史的地区种植的一种替代方法。嫁接与砧木的组合会影响西番莲农场的生产力和寿命。目的是评价北特拉诺瓦三种砧木嫁接西番槐的产量和死亡率。设计为随机区组,采用4种处理BRS Rubi do Cerrado (BRS- rc)苗木以及嫁接到3种砧木上的苗木:西番莲(Passiflora gibertii)、西番莲(Passiflora alata);西番莲nitida。通过裂接嫁接接穗,70天后进行种植。评价了果实的总数量和重量,以及植株的生产力和死亡率。结果表明,BRS-RC嫁接在赤杨和黑檀上的单果重、单果数和产量表现最佳。嫁接到不同砧木上的品种在幼苗方面表现出更高的生产力。嫁接到乳螺菌上的BRS-RC在16.5个月的培养过程中死亡率为零。在降低西番莲枯萎病对西番莲植株的致死率方面,乳香和赤霉素砧木优于alata砧木。在接种70日龄的BRS-RC接穗中,证实了在试验区分离到的passiflora oxysporum f.。
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引用次数: 0
Does the mycotoxin ingestion by beef heifers on feedlot change the productive parameters? 肉牛在饲养场摄入霉菌毒素是否会改变生产参数?
IF 0.5 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-03-31 DOI: 10.14393/bj-v39n0a2023-64329
Danielle de Oliveira Pião, M. Mello, M. M. D. Barbero, A. L. Silva, A. M. Moura, R. P. Barbero
In intensive beef cattle production systems, silage, corn, soy bean, and their coproducts are commonly used as feed. However, these ingredients are highly susceptible to contamination by fungi and mycotoxins, which may lead to immunological challenges and reduce animal production. The aim of the present study was to evaluate the effects of mycotoxin contamination of diet on intake, digestibility, and performance of heifers. Twenty non-pregnant (Nellore) heifers (age, >18 months; initial body weight, 348±30 kg) were used and randomly distributed in two treatments: (1) control (non-contaminated diet) and (2) zearalenone-contaminated diet (300 ppb). The diet comprised 70% corn silage and 30% concentrate. Individual dry matter intake and digestibility were estimated using external and internal markers. Heifer body weight was evaluated every week without fasting to calculate performance. The experimental design was completely randomized. Each animal was considered one experimental unit. Assumptions were tested for variance analyses (error normality, independence of errors, and homogeneity of variances) (p<0.05). There were no differences in dry matter intake (p=0.96) and digestibility (p=0.62). Performance (kg/day) did not vary as a function of zearalenone ingestion (p=0.68). Therefore, contamination of diet with 300 ppb zearalenone did not affect the intake, digestibility, and performance of feedlot-finished heifers.
在集约型肉牛生产系统中,青贮饲料、玉米、大豆及其副产品通常用作饲料。然而,这些成分极易受到真菌和真菌毒素的污染,这可能会导致免疫挑战并减少动物产量。本研究的目的是评估日粮中霉菌毒素污染对小母牛采食量、消化率和生产性能的影响。使用20只未怀孕(Nellore)小母牛(年龄>18个月;初始体重348±30kg),并将其随机分为两个处理:(1)对照组(无污染饮食)和(2)玉米赤霉烯酮污染饮食(300ppb)。日粮包括70%的玉米青贮饲料和30%的浓缩饲料。个体干物质摄入量和消化率使用外部和内部标记进行估计。在不禁食的情况下,每周对小母牛的体重进行评估,以计算其表现。实验设计完全随机。每只动物被视为一个实验单位。对方差分析的假设进行了检验(误差正态性、误差独立性和方差均匀性)(p<0.05)。干物质摄入量(p=0.96)和消化率(p=0.62)没有差异。性能(kg/d)与玉米赤霉烯酮摄入量无关(p=0.68),以及饲养场成品小母牛的性能。
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引用次数: 0
Antibacterial activity of Mangifera indica seed extracts combined with common antibiotics against multidrug-resistant Pseudomonas aeruginosa and Acinetobacter baumannii isolates 芒果籽提取物联合常用抗生素对多重耐药铜绿假单胞菌和鲍曼不动杆菌的抑菌活性
IF 0.5 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-03-31 DOI: 10.14393/bj-v39n0a2023-66760
Shadi Zeraatkar, Maedeh Tahan, Omefarveh Rostami, Alireza Neshani, Hadi Farsiani, Arezou Shahsavari, H. Safdari, Mahdi Hosseini Bafghi
In this project, we employed ethanolic (EMI) and aqueous (AMI) extracts of mango (Mangifera indica L., Anacardiaceae) fruit seeds as a modulator of antibiotic resistance against multidrug-resistant (MDR) Pseudomonas aeruginosa and Acinetobacter baumannii to evaluate natural compounds isolated from by-products or waste of edible plants. We also investigated the effect of these extracts alone and in combination with standard classes of antibiotics in the desired strains. M. indica seeds were processed and exploited using ethanol and water. The minimum inhibitory concentrations (MICs) of clinical isolates were examined against EMI and AMI extracts, followed by seven antibiotics of ceftazidime, ciprofloxacin, penicillin, amikacin, meropenem, ampicillin, and colistin. The checkerboard method evaluated the synergistic action between mango kernel extract (EMI) and seven antibiotics. EMI extract significantly revealed antimicrobial properties against MDR A. baumannii and P. aeruginosa with synergistic effects with the applied antibiotics. The considerable antibacterial efficacy of ethanolic extract of M. indica seeds can have great curative value as antibacterial drugs against infections caused by MDR P.aeruginosa and A. baumannii.
在本项目中,我们利用芒果(Mangifera indica L., anac心科)果实种子的乙醇(EMI)和水(AMI)提取物作为对多药耐药(MDR)铜绿假单胞菌和鲍曼不动杆菌的耐药性调节剂,对从食用植物副产品或废物中分离的天然化合物进行了评价。我们还研究了这些提取物单独使用和与标准类别抗生素联合使用的效果。利用乙醇和水对籼稻种子进行加工和开发。临床分离菌株对EMI和AMI提取物的最低抑菌浓度(mic),其次是头孢他啶、环丙沙星、青霉素、阿米卡星、美罗培南、氨苄西林和粘菌素7种抗生素。采用棋盘法评价芒果仁提取物(EMI)与7种抗生素的协同作用。EMI提取物对耐多药鲍曼杆菌和铜绿假单胞菌具有显著的抗菌作用,并与应用抗生素具有协同效应。籼米种子乙醇提取物具有较强的抗菌作用,作为耐多药铜绿假单胞菌和鲍曼假单胞菌感染的抗菌药物具有很大的治疗价值。
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引用次数: 0
Quality of raw bulk-tank milk produced in northeast Brazil 巴西东北部生产的原料散装罐牛奶的质量
IF 0.5 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-03-31 DOI: 10.14393/bj-v39n0a2023-59825
V. M. D. Araújo, S. Barbosa, A. H. Rangel, Luís H F Borba, Juliana Oliveira, Â. Batista
The objective of this study was to evaluate the seasonal influence on the chemical composition, somatic cell count (SCC), and total bacterial count (TBC) of raw bulk-tank milk in northeastern Brazilian states. Data were obtained from milk samples from tanks collected monthly by industries registered with the Federal Inspection Service. According to normative instruction #62 (IN-62), two validity periods were considered. The highest recorded averages for chemical composition were between May and July. The mean fat content varied from 3.51% to 3.69%, and the protein content ranged from 3.07% to 3.17%. The averages of SCC ranged from 4.66 to 4.90 × 1,000 cells/ml, with the highest being recorded in July. At the same time, the TBC ranged from 2.34 to 2.53 cfu/ml. The highest TBC was recorded in March. The mean values of fat, protein, defatted dry extract, SCCs, and TBC were influenced by the months of the year. The means for these variables decreased in periods when Brazilian legislation was more severe. However, the SCC and TBC averages found in this study were still high, considering the quality of raw milk production. SCC and TBC presence still did not comply with the limits established by the legislation.
本研究的目的是评估季节对巴西东北部各州原料散装罐牛奶的化学成分、体细胞计数(SCC)和细菌总数(TBC)的影响。数据来自在联邦检验局注册的行业每月收集的牛奶样本。根据规范指令#62 (IN-62),考虑了两个有效期。化学成分的最高记录平均值出现在5月至7月之间。平均脂肪含量为3.51% ~ 3.69%,蛋白质含量为3.07% ~ 3.17%。SCC平均值为4.66 ~ 4.90 × 1000细胞/ml, 7月最高。TBC为2.34 ~ 2.53 cfu/ml。最高的TBC记录是在3月份。脂肪、蛋白质、脱脂干提取物、SCCs和TBC的平均值受月份的影响。在巴西立法较为严格的时期,这些变量的平均数减少了。然而,考虑到原料奶的质量,本研究中发现的SCC和TBC平均值仍然很高。SCC和TBC的存在仍然不符合立法规定的限制。
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引用次数: 0
Tifton 85 grass performance following chemical and organic fertilizers 施用化学和有机肥料后的蒂夫顿85草的生长性能
IF 0.5 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-03-31 DOI: 10.14393/bj-v39n0a2023-63730
Naiara Taís Alves da Silva, A. Teixeira, L. Gonçalves, D. Ottoni, L. Cavalcanti, Thaís Figueiredo Pereira, Flávia Cristina de Oliveira e Silva, D. Jayme
The Tifton 85 grass offers high yield and nutritional value. It is demanding in soil fertility and highly responsive to fertilization. However, the response to fertilization can be affected by the source, dose, and installment of fertilizers, as well as environmental conditions. Evaluations of the joint application of chemical and organic fertilizers are scarce. Therefore, this study aimed to evaluate the effect of chemical and organic fertilization on agronomic characteristics, mineral use efficiencies, and bromatological composition of Tifton 85 grass, and determine fertilization strategies to optimize the yield and nutritional value of Tifton 85 grass. The findings were influenced by grass cuttings. The highest values for height and accumulated dry matter productivity were obtained with the highest fertilizer doses. The leaf/stem ratio was not affected by fertilization. The highest mineral use efficiencies were obtained with 200 and 400 kg of N/ha/year of NPK 20-00-20 formulation and up to 10 t/ha/year of poultry litter (PL), and these values decreased when applying high PL doses. The crude protein content increased, while the contents of neutral detergent fiber and acid detergent fiber decreased. The protein fractions and in vitro dry matter digestibility were not affected by fertilization. The potassium content increased with high fertilizer doses. Applying 350 to 400 kg of N/ha/year of NPK 20-00-20 formulation and 25 to 30 t/ha/year of PL promoted the highest accumulated dry matter productivity. Applying 200 to 400 kg of N/ha/year of NPK 20-00-20 formulation and 10/ha/year of PL is recommended for optimizing mineral use efficiencies. As for bromatological composition, increasing the doses of both fertilizers was beneficial in reducing fiber contents, but only organic fertilization increased crude protein content.
蒂夫顿85草产量高,营养价值高。它对土壤肥力要求高,对施肥反应灵敏。然而,对施肥的反应可能受到肥料的来源、剂量和施用以及环境条件的影响。对化肥和有机肥联合施用的评价很少。因此,本研究旨在评价化学和有机施肥对Tifton 85草农艺性状、矿物利用效率和土壤成分的影响,并确定施肥策略,以优化Tifton 85草的产量和营养价值。这些发现受到了割草的影响。高和累积干物质生产力随施肥量的增加而增加。叶片/茎比不受施肥影响。施用200和400公斤N/公顷/年的氮磷钾20-00-20配方以及高达10吨/公顷/年的家禽垃圾(PL)时,矿物质利用效率最高,当施用高PL剂量时,这些数值下降。粗蛋白质含量升高,中性洗涤纤维和酸性洗涤纤维含量降低。蛋白质组分和体外干物质消化率不受受精的影响。钾肥用量越大,钾含量越高。施用氮磷钾20-00-20配方350 ~ 400 kg /公顷/年和施用有机肥25 ~ 30 t/公顷/年可促进最高的累积干物质生产力。建议施用200至400公斤N/公顷/年的氮磷钾20-00-20配方和10/公顷/年的PL以优化矿物利用效率。在土壤成分方面,增加两种肥料的用量均有利于降低纤维含量,但只有有机肥能提高粗蛋白质含量。
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引用次数: 0
Preparation and characterization of steam and CO2 activated carbon from Brazil nut shell 巴西坚果壳蒸汽活性炭和CO2活性炭的制备及表征
IF 0.5 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-03-31 DOI: 10.14393/bj-v39n0a2023-64438
Pryscila Machado de Castro, Stela Regina Ferrarini, Michelle Ferreira da Silva Rimoli, A. A. Merlo, R. M. Nogueira, E. M. Pires
Activated carbon is a porous material with several industrial applications. In this research, brazil nut shell was used to produce activated carbon, a promising raw material based on its low cost and high quality. Also, nut processing generates an extensive amount of waste, requiring proper disposal. The carbon was produced at temperatures of 600, 700, and 800 °C and activated by physical method using steam or CO2. Yield was calculated, and the activated carbon was analyzed according to the following physical-chemical parameters: water content, volatile material, fixed carbon, ash, apparent density, and pH. Infrared spectroscopy and BET adsorption isotherms were also performed. The fixed carbon contents of the carbons produced at 700 and 800 °C remained above 84%. The pH values characterized them as basic. The surface areas of the carbon obtained at 800 °C were 397 ± 8 m2 g–1 and 325 ± 7 m2 g–1 in those which were activated by steam and CO2, respectively.
活性炭是一种多孔材料,具有多种工业用途。本研究以巴西坚果壳为原料,以其低成本、高质量的特点制备活性炭。此外,坚果加工产生大量的废物,需要适当的处理。碳在600、700和800°C的温度下产生,并通过蒸汽或二氧化碳的物理方法活化。计算产率,根据含水量、挥发性物质、固定碳、灰分、表观密度、ph等理化参数对活性炭进行分析,并进行红外光谱分析和BET吸附等温线分析。在700℃和800℃生产的碳的固定碳含量保持在84%以上。pH值表明它们是碱性的。水蒸气活化和CO2活化的活性炭在800℃时得到的比表面积分别为397±8 m2 g-1和325±7 m2 g-1。
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引用次数: 0
Proposal of a non-linear model to adjust in vitro gas production at different incubation times 提出一种非线性模型来调整不同孵育时间的体外产气量
IF 0.5 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-03-31 DOI: 10.14393/bj-v39n0a2023-63017
A. L. B. D. Santos, T. A. Ferreira, C. Brito, G. R. Moreira, Frank Gomes-Silva, J. Jale, R. B. Reis, Leonardo Andrade Leite, P. G. Pimentel
This work aims to propose a new model named Gompertz-Von Bertalanffy bicompartmental (GVB), a combination of the models Gompertz and Von Bertalanffy. The GVB models is applied to fit the kinetic curve of cumulative gas production (CGP) of four foods (SS – sunflower silage; CS – corn silage; and the mixtures 340SS – 660 gkg-1 of corn silage and 340 gkg-1 of sunflower silage; and 660SS – 340 gkg-1 of corn silage and 660 gkg-1 of sunflower silage). The GVB fit is compared to models Logistic-Von Bertalanffy bicompartmental (LVB) and bicompartmental logistic (BL). All the process studied employed the semi-automatic “in vitro” technique of producing gases used in ruminant nutrition. The gas production readout was performed at times 2, 4, 6, 8, 10, 12, 15, 19, 24, 30, 48, 72, and 96 h. The data generated were used to estimate the models’ parameters by the least squared method with the iterative Gauss-Newton process. The data fit quality of the models was verified using the adjusted coefficient of determination criterion (), mean residual square (MRS), Akaike information criterion (AIC), and mean absolute deviation (MAD). Among the analyzed models, the LVB model presented the best quality of fit evaluators for CS. In contrast, the GVB model showed better quality of fit to describe CGP over time for 340SS, 660SS, and SS, presenting the highest values of () and the lowest values of MSR, AIC, and MAD.
本工作旨在提出一种新的模型,命名为Gompertz-Von-Bertalanffy双腔(GVB),该模型是Gompertz和Von-Bertalanffy模型的组合。GVB模型用于拟合四种食物(SS–向日葵青贮饲料;CS–玉米青贮饲料;以及玉米青贮饲料340SS–660 gkg-1和向日葵青贮饲料340 gkg-1的混合物;玉米青贮饲料660SS–340 gkg-2和向日葵青贮料660 gkg-1)的累积产气(CGP)动力学曲线。将GVB拟合与模型Logistic-Von-Bertalanffy双腔(LVB)和双腔Logistic(BL)进行比较。所有研究的过程都采用了半自动的“体外”技术来生产反刍动物营养中使用的气体。在第2、4、6、8、10、12、15、19、24、30、48、72和96小时进行天然气产量读数。生成的数据用于通过最小二乘法和迭代高斯-牛顿过程估计模型的参数。使用调整后的决定系数准则()、均残差平方(MRS)、Akaike信息准则(AIC)和平均绝对偏差(MAD)验证了模型的数据拟合质量。在所分析的模型中,LVB模型为CS提供了最佳的拟合质量评估器。相反,GVB模型在描述340SS、660SS和SS随时间变化的CGP时显示出更好的拟合质量,呈现出()的最高值和MSR、AIC和MAD的最低值。
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引用次数: 0
Anatomical, morphogenic and structural characteristics of Xaraés palisade grass under grazing 放牧条件下沙拉萨草的解剖、形态及结构特征
IF 0.5 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-03-31 DOI: 10.14393/bj-v39n0a2023-60937
Kelen Cristina Basso, L. Galzerano, W. L. Silva, A. Ruggieri, R. Reis
This study evaluated under grazing intensities and periods of the year: leaf anatomy of Urochloa brizantha cv. Xaraés and its correlation with morphogenetic and structural characteristics, and leaves degradation after in situ incubation. Treatments were four grazing intensities (GI) defined by the pasture residuals leaf area index (rLAI 0.8, 1.3, 1.8, and 2.3) with three replications in a completely randomized design. Cows grazed in a rotational stocking with pastures regrowth period determined by 95% light interception. Leaves showed a higher proportion of sclerenchyma (2.64%) in pastures under lower GI and in the dry season (2.42%). Pastures managed under higher GI showed lower number of expanded leaves (2.58), lower number of lives leaves (3.45), and lower leaf senescence rate (0.05 cm tiller−1 d−1). Positive correlation was observed between leaf elongation rate and adaxial epidermis and vascular tissues. rLAI 1.8 and 2.3 provided greater residues after in situ leaf incubation at times 12, 48, 72, and 96 h compared to rLAI 0.8 and 1.3. rLAI and period of the year had little influence on leaf anatomy of the Xaraés managed under 95% LI, and leaf anatomy is correlated with the morphogenetic and structural pasture characteristics. Pastures managed under lower GI show more residues after leaves incubation in rumen.
本研究评估了不同放牧强度和不同放牧时段下的毛斑草叶片解剖结构。xarasamys及其与形态发生、结构特征及原位培养后叶片降解的关系。采用完全随机设计,采用牧场残叶面积指数(rLAI) 0.8、1.3、1.8和2.3定义的4个放牧强度(GI), 3个重复处理。奶牛在轮换放养中放牧,牧场再生期由95%的光拦截决定。低GI牧草叶片中厚壁组织的比例(2.64%)高于旱季(2.42%)。在高GI条件下,牧草的展开叶数(2.58)、活叶数(3.45)和叶片衰老率(0.05 cm分蘖−1 d−1)均较低。叶片伸长率与近轴表皮和维管组织呈显著正相关。与rLAI 0.8和1.3相比,rLAI 1.8和2.3在叶片原位孵育12、48、72和96 h后提供了更多的残留物。在95% LI条件下,叶面积和季节对沙沙茅叶片解剖结构影响不大,叶片解剖结构与形态发生和结构特征相关。在低GI条件下管理的牧场,瘤胃中叶片孵化后的残留物较多。
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引用次数: 0
Inhibitory effects of diarsenic trioxide (As2O3) on hepatocellular carcinoma cells exerted by regulation of promyelocytic leukemia protein levels 三氧化二砷通过调节早幼粒细胞白血病蛋白水平对肝癌细胞的抑制作用
IF 0.5 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-03-31 DOI: 10.14393/bj-v39n0a2023-63086
Guowu Zhang, Wen Wang, Yukai Jin, Shi Jin, Lei Mi, Xiaowen Song, He Li, J. Liao
Previous Chinese research revealed that diarsenic trioxide (As2O3) inhibits acute promyelocytic leukemia (PML) cell proliferation and initiates apoptosis through degradation of the PML-retinoic acid receptor protein. This study was to analyse whether As2O3 also had an effect on hepatocellular carcinoma (HCC) cells. As2O3 effects on various HCC cell lines and primary HCC cells were investigated in time and dose series, including measurements of cell growth, PML mRNA and protein expression, xenografted tumor formation, and the self-renewal Oct4 and hepatocyte marker expressions in mouse model xenografts or cells treated with PML siRNA. The results were analyzed by immunocytochemistry, quantitative reverse transcription PCR and western blotting as well as indocyanine green and Periodic Acid Schiff staining. As2O3 inhibited HCC cell and HCC cell-derived xenograft tumor formation in a time-dependent manner and reduced PML protein expression in HCC cells, but had limited effects on PML mRNA levels in cell nuclei. The HCC cell line HuH7 treated with As2O3 showed a decreased expression of alpha-fetoprotein and increased expression and transcription of mature hepatocyte markers, indicating differentiation of HCC cells into hepatocytes. Cytokeratin 18 protein and mRNA levels as well as tyrosine aminotransferase and apolipoprotein B mRNA transcriptions were enhanced by As2O3 as were the numbers of indocyanine green and Periodic Acid Schiff stained cells. In addition, As2O3 downregulated the expression of Oct4. In conclusion, since As2O3 inhibited HCC cell proliferation and HCC cell-derived xenograft tumor formation it is suggested that an appropriate concentration of As2O3 might be a promising therapy to treat HCC.
中国先前的研究表明,三氧化二砷(As2O3)通过降解急性早幼粒细胞白血病(PML)视黄酸受体蛋白来抑制细胞增殖并引发细胞凋亡。本研究旨在分析As2O3是否对肝细胞癌(HCC)细胞也有影响。以时间和剂量序列研究了As2O3对各种HCC细胞系和原代HCC细胞的影响,包括在小鼠模型异种移植物或用PML-siRNA处理的细胞中测量细胞生长、PML-mRNA和蛋白表达、异种移植物肿瘤形成以及自我更新的Oct4和肝细胞标志物表达。免疫细胞化学、定量逆转录聚合酶链式反应、蛋白质印迹、吲哚青绿和碘酸希夫染色对结果进行分析。As2O3以时间依赖的方式抑制HCC细胞和HCC细胞衍生的异种移植物肿瘤的形成,并降低HCC细胞中PML蛋白的表达,但对细胞核中PML mRNA水平的影响有限。用As2O3处理的HCC细胞系HuH7显示出甲胎蛋白的表达降低,成熟肝细胞标志物的表达和转录增加,表明HCC细胞分化为肝细胞。As2O3增强了细胞角蛋白18蛋白和mRNA水平以及酪氨酸氨基转移酶和载脂蛋白B mRNA转录,吲哚青绿和周期酸Schiff染色的细胞数量也增强了。此外,As2O3下调了Oct4的表达。总之,由于As2O3抑制HCC细胞增殖和HCC细胞衍生的异种移植物肿瘤形成,因此建议适当浓度的As2O3可能是治疗HCC的一种有前途的治疗方法。
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