Pub Date : 2023-03-10DOI: 10.14393/bj-v39n0a2023-64399
João Vitor Lopes-Ferreira, Jullyana Costa Machado, F. C. Dias, Luciana S Altoé, R. Vilela, S. D. Matta, Angelica de Oliveira Gomes, M. Gomes
Broad-spectrum antimicrobial doxycycline acts as an inhibitor of protein synthesis and it is widely used in the clinical treatment of various infections by microorganisms that are sensitive to the drug, as well as in animal feed. Its liposolubility guarantees its high tissue bioavailability, being associated with several biochemical changes in the organism and potentially adverse effects on reproduction. This study aims to evaluate the effects of the action of doxycycline on spermatogenesis to provide a complete analysis of the tubular and interstitial compartments and to identify possible changes in the testicular parenchyma. Adult male Wistar rats were divided into three groups: one control (water), and two treated with doxycycline at the doses of 10mg/kg and 30mg/kg, for 30 days. After euthanasia and sample processing, the following parameters were evaluated: a) tubular diameter and height of the seminiferous epithelium; b) volumetric proportions (%) and volumes (mL) of the components of the testicular parenchyma; c) counting testicular germ cell populations; d) evaluation of cell viability. The results of the comparative evaluation between the experimental groups demonstrated a significant increase in the diameter and area of the tubular lumen and a reduction in the count of spermatogonia in the experimental group that received doxycycline hyclate at a dose of 30mg/kg. In the same experimental group, an increase in the overall yield of spermatogenesis was found as a consequence of the increase in the mitotic index.
{"title":"Doxycycline hyclate may damage testicular germinal epithelium in Wistar rats","authors":"João Vitor Lopes-Ferreira, Jullyana Costa Machado, F. C. Dias, Luciana S Altoé, R. Vilela, S. D. Matta, Angelica de Oliveira Gomes, M. Gomes","doi":"10.14393/bj-v39n0a2023-64399","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-64399","url":null,"abstract":"Broad-spectrum antimicrobial doxycycline acts as an inhibitor of protein synthesis and it is widely used in the clinical treatment of various infections by microorganisms that are sensitive to the drug, as well as in animal feed. Its liposolubility guarantees its high tissue bioavailability, being associated with several biochemical changes in the organism and potentially adverse effects on reproduction. This study aims to evaluate the effects of the action of doxycycline on spermatogenesis to provide a complete analysis of the tubular and interstitial compartments and to identify possible changes in the testicular parenchyma. Adult male Wistar rats were divided into three groups: one control (water), and two treated with doxycycline at the doses of 10mg/kg and 30mg/kg, for 30 days. After euthanasia and sample processing, the following parameters were evaluated: a) tubular diameter and height of the seminiferous epithelium; b) volumetric proportions (%) and volumes (mL) of the components of the testicular parenchyma; c) counting testicular germ cell populations; d) evaluation of cell viability. The results of the comparative evaluation between the experimental groups demonstrated a significant increase in the diameter and area of the tubular lumen and a reduction in the count of spermatogonia in the experimental group that received doxycycline hyclate at a dose of 30mg/kg. In the same experimental group, an increase in the overall yield of spermatogenesis was found as a consequence of the increase in the mitotic index.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43438430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-10DOI: 10.14393/bj-v39n0a2023-65597
A. G. Guimarães, G. Ceccon, D. P. Capristo, O. H. Oliveira, M. Retore, Adriano dos Santos
Maize silage has been used as a forage reserve strategy for critical periods or continuous use in animal feed. However, new genotypes and their potential must be identified. Thus, this study aimed to evaluate the potential of maize genotypes for silage and grain in one off-season in the midwest region of Brazil, under limited water and frost, and select them for this dual purpose (silage and grain) using the GT Biplot tool. The experiment was performed at Embrapa Western Agriculture in the autumn-winter season of 2021 in Dourados, Mato Grosso do Sul, Brazil. The experimental design consisted of randomized blocks of six maize genotypes (BRS1010, KWS9606, 1P2224, 1Q2383, BRS3046, and CAPO) with five replications under no-tillage. Silage points were evaluated at harvest when the grain milk line was at ¾ and maize grains at the maturation stage (dry plant). The 1P2224 and 1Q2383 maize genotypes present silage (high green and dry biomass) and grain yield potential. The GT Biplot tool identified the 1P2224 genotype as superior and suitable for cultivation or as a parent in a breeding program in the midwest region of Brazil for silage and grain yield evaluations of one off-season under limited water and frost.
玉米青贮已被用作关键时期或连续使用的饲料储备策略。然而，必须确定新的基因型及其潜力。因此，本研究旨在评估巴西中西部地区一个淡季在有限水和霜冻条件下青贮和谷物玉米基因型的潜力，并使用GT Biplot工具选择用于这种双重用途(青贮和谷物)的玉米。该实验于2021年秋冬季节在巴西南马托格罗索州杜拉多斯的Embrapa Western Agriculture进行。试验设计采用6个玉米基因型(BRS1010、KWS9606、1P2224、1Q2383、BRS3046和CAPO)随机区，5个重复，免耕。青贮点是在收获时谷物乳线为3 / 4时和玉米籽粒成熟期(干株)时进行评价的。1P2224和1Q2383玉米基因型具有青贮(高绿生物量和高干生物量)和籽粒产量潜力。GT Biplot工具鉴定出1P2224基因型优越，适合栽培或作为亲本在巴西中西部地区的育种计划中进行青贮和一个淡季在限水限霜条件下的粮食产量评估。
{"title":"Yield potential and selection of off-season maize for silage and grain using GT Biplot under limited water and frost","authors":"A. G. Guimarães, G. Ceccon, D. P. Capristo, O. H. Oliveira, M. Retore, Adriano dos Santos","doi":"10.14393/bj-v39n0a2023-65597","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-65597","url":null,"abstract":"Maize silage has been used as a forage reserve strategy for critical periods or continuous use in animal feed. However, new genotypes and their potential must be identified. Thus, this study aimed to evaluate the potential of maize genotypes for silage and grain in one off-season in the midwest region of Brazil, under limited water and frost, and select them for this dual purpose (silage and grain) using the GT Biplot tool. The experiment was performed at Embrapa Western Agriculture in the autumn-winter season of 2021 in Dourados, Mato Grosso do Sul, Brazil. The experimental design consisted of randomized blocks of six maize genotypes (BRS1010, KWS9606, 1P2224, 1Q2383, BRS3046, and CAPO) with five replications under no-tillage. Silage points were evaluated at harvest when the grain milk line was at ¾ and maize grains at the maturation stage (dry plant). The 1P2224 and 1Q2383 maize genotypes present silage (high green and dry biomass) and grain yield potential. The GT Biplot tool identified the 1P2224 genotype as superior and suitable for cultivation or as a parent in a breeding program in the midwest region of Brazil for silage and grain yield evaluations of one off-season under limited water and frost.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46168048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-10DOI: 10.14393/bj-v39n0a2023-65096
Alluanan Adelson do Nascimento Silva, J. M. D. Oliveira, F. C. Dias, R. N. Moraes, Elizabeth Neves de Melo, P. Soares, V. A. Júnior
The effects of systemic insulin administration at different concentrations on the testicular tissue of diabetic adult rats, induced by streptozotocin, are evaluated by the morphological analysis of spermatogenic process. Twenty-four adult male rats were divided into 1) Control Group: they received citrate buffer, by intraperitoneal injection; 2) Diabetic Group: induced by intraperitoneal injection of streptozotocin (60 mg. kg-1 of body weight); 3) Insulin 50%: induced diabetes treated with half of standard dosage of insulin; 4) Insulin 100%: induced diabetes treated with standard dose of insulin. After eight weeks, animals were weighted and anesthetized; testicles were removed and processed in resin. Body and testicular weight of diabetic rats decreased when compared to that of control. Parameters increased with insulin therapy. Testosterone levels were low in diabetic animals but rates recovered after insulin therapy. Nuclear diameter and volume of Leydig cells decreased in diabetic rats although they significantly increased after insulin therapy. Results showed that the administration of insulin in diabetic rats promoted a protective effect of testicular parenchyma, enhancing efficient recovery on testosterone levels and increase in daily sperm production.
{"title":"The effectiveness of insulin therapy in the functional and morphological recovery of leydig cells in rats submitted to Diabetes mellitus","authors":"Alluanan Adelson do Nascimento Silva, J. M. D. Oliveira, F. C. Dias, R. N. Moraes, Elizabeth Neves de Melo, P. Soares, V. A. Júnior","doi":"10.14393/bj-v39n0a2023-65096","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-65096","url":null,"abstract":"The effects of systemic insulin administration at different concentrations on the testicular tissue of diabetic adult rats, induced by streptozotocin, are evaluated by the morphological analysis of spermatogenic process. Twenty-four adult male rats were divided into 1) Control Group: they received citrate buffer, by intraperitoneal injection; 2) Diabetic Group: induced by intraperitoneal injection of streptozotocin (60 mg. kg-1 of body weight); 3) Insulin 50%: induced diabetes treated with half of standard dosage of insulin; 4) Insulin 100%: induced diabetes treated with standard dose of insulin. After eight weeks, animals were weighted and anesthetized; testicles were removed and processed in resin. Body and testicular weight of diabetic rats decreased when compared to that of control. Parameters increased with insulin therapy. Testosterone levels were low in diabetic animals but rates recovered after insulin therapy. Nuclear diameter and volume of Leydig cells decreased in diabetic rats although they significantly increased after insulin therapy. Results showed that the administration of insulin in diabetic rats promoted a protective effect of testicular parenchyma, enhancing efficient recovery on testosterone levels and increase in daily sperm production.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48424670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-03DOI: 10.14393/bj-v39n0a2023-62577
Jailson do Nascimento Silva, Amanda Camila dos Santos Linhares, D. A. Palmieri, Marcones Ferreira Costa, G. H. Sá, M. Gomes, M. F. Santos, L. Feitoza, Sérgio Emílio Dos Santos Valente
Molecular markers are important tools in the characterization of plant genetic diversity and can provide support for conservation strategies for endangered populations. The different molecular techniques involve the evaluation of many individuals; therefore, it is crucial to have fast, efficient, and inexpensive methods for DNA extraction. Given the importance of the Aroeira (Myracrodruon urundeuva Fr. All.) it is pertinent to optimize a protocol that allows the obtainment of intact and pure DNA, aiming to assist conservation strategies for this species that is threatened with extinction. Thus, this study aimed to compare five DNA extraction methods: Dellaporta et al. (1983), Doyle and Doyle (1987) modified, Ferreira and Grattapaglia (1995), Romano and Brasileiro (2015), and Khanuja et al. (1999) and optimize the most efficient protocol for M. urundeuva. The modified DNA extraction protocol proposed by Doyle and Doyle (1987), using 100 mg of leaf tissue and 6 µl of β-mercaptoethanol was the protocol that presented the sharpest bands after DNA electrophoresis and after the reactions of amplification employing Polymerase Chain Reaction (PCR). Therefore, it is suggested to use the protocol described by Doyle and Doyle (1987) modified for the extraction of DNA from young M. urundeuva leaves to carry out techniques involving molecular markers.
{"title":"Optimized method for dna extraction and PCR amplification in aroeira tree","authors":"Jailson do Nascimento Silva, Amanda Camila dos Santos Linhares, D. A. Palmieri, Marcones Ferreira Costa, G. H. Sá, M. Gomes, M. F. Santos, L. Feitoza, Sérgio Emílio Dos Santos Valente","doi":"10.14393/bj-v39n0a2023-62577","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-62577","url":null,"abstract":"Molecular markers are important tools in the characterization of plant genetic diversity and can provide support for conservation strategies for endangered populations. The different molecular techniques involve the evaluation of many individuals; therefore, it is crucial to have fast, efficient, and inexpensive methods for DNA extraction. Given the importance of the Aroeira (Myracrodruon urundeuva Fr. All.) it is pertinent to optimize a protocol that allows the obtainment of intact and pure DNA, aiming to assist conservation strategies for this species that is threatened with extinction. Thus, this study aimed to compare five DNA extraction methods: Dellaporta et al. (1983), Doyle and Doyle (1987) modified, Ferreira and Grattapaglia (1995), Romano and Brasileiro (2015), and Khanuja et al. (1999) and optimize the most efficient protocol for M. urundeuva. The modified DNA extraction protocol proposed by Doyle and Doyle (1987), using 100 mg of leaf tissue and 6 µl of β-mercaptoethanol was the protocol that presented the sharpest bands after DNA electrophoresis and after the reactions of amplification employing Polymerase Chain Reaction (PCR). Therefore, it is suggested to use the protocol described by Doyle and Doyle (1987) modified for the extraction of DNA from young M. urundeuva leaves to carry out techniques involving molecular markers.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44597548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-24DOI: 10.14393/bj-v39n0a2023-63353
D. Agostinetto, Daniela Tessaro, M. F. Schmitz, M. Martins, L. Vargas, A. Ulguim
Resistance to acetolactate synthase (ALS) inhibitors have increased recently in South Brazil where the major weeds of flooded rice (barnyardgrass and weedy rice) have evolved resistance to imazapyr+imazapic. The aim of this research was to evaluate a growth medium for tissue regeneration of tillers in barnyardgrass, as well as an agar-based bioassays test (also from tillers) to detect susceptible and resistant biotypes of weedy rice and barnyardgrass to imazapyr+imazapic in vitro. Greenhouse experiments were conducted to detect ALS-resistant (R) and susceptible (S) weedy rice and barnyardgrass biotypes, and bioassays were carried out to evaluate an adequate growth medium for barnyardgrass tiller regeneration and determine the concentration of herbicide to distinguish R and S plants. The culture medium that provided a suitable barnyardgrass growth was MS 50% with the addition of benzylamino-purine. The tissue regeneration in vitro with the growth medium containing imazapyr+imazapic allowed to discriminate between R and S barnyardgrass and weedy rice plants. The concentration required for satisfactory control of susceptible barnyardgrass and weedy rice explants grown in vitro was 0.9 μM and 1.3 μM of imazapyr+imazapic herbicide, respectively. The bioassay in vitro using tiller regeneration provides an opportunity to predict effectively imazapyr+imazapic resistance in barnyardgrass and weedy rice.
{"title":"Quick bioassay test from tillers for detecting ALS herbicide resistance of weedy rice and barnyardgrass","authors":"D. Agostinetto, Daniela Tessaro, M. F. Schmitz, M. Martins, L. Vargas, A. Ulguim","doi":"10.14393/bj-v39n0a2023-63353","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-63353","url":null,"abstract":"Resistance to acetolactate synthase (ALS) inhibitors have increased recently in South Brazil where the major weeds of flooded rice (barnyardgrass and weedy rice) have evolved resistance to imazapyr+imazapic. The aim of this research was to evaluate a growth medium for tissue regeneration of tillers in barnyardgrass, as well as an agar-based bioassays test (also from tillers) to detect susceptible and resistant biotypes of weedy rice and barnyardgrass to imazapyr+imazapic in vitro. Greenhouse experiments were conducted to detect ALS-resistant (R) and susceptible (S) weedy rice and barnyardgrass biotypes, and bioassays were carried out to evaluate an adequate growth medium for barnyardgrass tiller regeneration and determine the concentration of herbicide to distinguish R and S plants. The culture medium that provided a suitable barnyardgrass growth was MS 50% with the addition of benzylamino-purine. The tissue regeneration in vitro with the growth medium containing imazapyr+imazapic allowed to discriminate between R and S barnyardgrass and weedy rice plants. The concentration required for satisfactory control of susceptible barnyardgrass and weedy rice explants grown in vitro was 0.9 μM and 1.3 μM of imazapyr+imazapic herbicide, respectively. The bioassay in vitro using tiller regeneration provides an opportunity to predict effectively imazapyr+imazapic resistance in barnyardgrass and weedy rice.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47826844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-24DOI: 10.14393/bj-v39n0a2023-63062
R. L. Ferraz, P. S. Costa, Guilherme Felix Dias, José Oscar da Rocha Silva, P. R. A. Viégas, A. D. S. Medeiros, José Dantas Neto, A. S. Melo
Weeds negatively influence agricultural production. However, those losses depend on weed specie, its time of emergence, and period of interference on agricultural crops. Synthetic herbicides are commonly used to control these plants species; however, they may cause damage to the environment, human beings and animals health, and this problem justify the need to develop alternative bioherbicides. To evaluate the allelopathic potential of Ricinus communis (Castor bean) and light spectrum variation on the emergence and growth of Cyperus rotundus L., a trial was carried out in a protected environment with 15% of brightness reduction at the Center for Agricultural and Environmental Sciences at the Paraíba State University. Four aqueous extract concentrations of R. communis leaves were tested (0, 5, 10, and 15%) and four light spectrums variations (white, purple, blue, and red lights). Variables such as emergence, length, dry matter accumulation and growth rates of shoots and root of C. rotundus seedlings were assessed. Data were analyzed by normality test, analysis of variance, polynomial regression, and averages test. Soot and root emergence, length, and dry matter accumulation of C. rotundus seedlings were reduced due to the allelopathy caused by R. communis aqueous extract leaves (15% concentration) and under purple or red light spectrum radiation.
{"title":"Allelopathy of Ricinus communis and light spectrum variation decrease emergence and growth of Cyperus rotundus","authors":"R. L. Ferraz, P. S. Costa, Guilherme Felix Dias, José Oscar da Rocha Silva, P. R. A. Viégas, A. D. S. Medeiros, José Dantas Neto, A. S. Melo","doi":"10.14393/bj-v39n0a2023-63062","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-63062","url":null,"abstract":"Weeds negatively influence agricultural production. However, those losses depend on weed specie, its time of emergence, and period of interference on agricultural crops. Synthetic herbicides are commonly used to control these plants species; however, they may cause damage to the environment, human beings and animals health, and this problem justify the need to develop alternative bioherbicides. To evaluate the allelopathic potential of Ricinus communis (Castor bean) and light spectrum variation on the emergence and growth of Cyperus rotundus L., a trial was carried out in a protected environment with 15% of brightness reduction at the Center for Agricultural and Environmental Sciences at the Paraíba State University. Four aqueous extract concentrations of R. communis leaves were tested (0, 5, 10, and 15%) and four light spectrums variations (white, purple, blue, and red lights). Variables such as emergence, length, dry matter accumulation and growth rates of shoots and root of C. rotundus seedlings were assessed. Data were analyzed by normality test, analysis of variance, polynomial regression, and averages test. Soot and root emergence, length, and dry matter accumulation of C. rotundus seedlings were reduced due to the allelopathy caused by R. communis aqueous extract leaves (15% concentration) and under purple or red light spectrum radiation.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45068718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-24DOI: 10.14393/bj-v39n0a2023-63174
A. Krisnawati, M. M. Adie, R. Krisdiana, Y. Prayogo, R. Soehendi, Y. Baliadi
Whiteflies are a severe threat to soybean production in the tropics. This study aimed to evaluate the soybean resistance level of the whitefly Bemisia tabaci in controlled and uncontrolled environments that is associated with plant age, damage intensity, and trichome density. The research was conducted under two conditions: non-sprayed (NS) and sprayed (SP). This study used 50 soybean genotypes arranged in a randomized block design with three replicates. The whitefly population was derived from natural infestations. The results showed that the highest wild population of B. tabaci occurred at 40 days after planting (DAP), i.e., 126.08 adults/plant in the NS environment and 22.57 adults/plant in the SP environment. The peak damage intensity occurred at 50 DAP, 20.71% in the NS environment, and 17.15% in the SP environment. In the NS environment, there were six resistant genotypes (including the resistant control G100H), 25 moderate, and 19 susceptible genotypes. In the SP environment, 19 genotypes were resistant, 22 genotypes were moderate, and nine genotypes were susceptible, respectively. Six soybean genotypes showed consistent resistance to B. tabaci in NS and SP environments. The low density of leaf trichomes in soybean may influence the high resistance to B. tabaci. The resistant genotypes identified in this study could be utilized in breeding programs for B. tabaci resistance.
{"title":"Differential levels of soybean resistance to the whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) under controlled and uncontrolled environments are associated with plant age, damage intensity, and trichome density","authors":"A. Krisnawati, M. M. Adie, R. Krisdiana, Y. Prayogo, R. Soehendi, Y. Baliadi","doi":"10.14393/bj-v39n0a2023-63174","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-63174","url":null,"abstract":"Whiteflies are a severe threat to soybean production in the tropics. This study aimed to evaluate the soybean resistance level of the whitefly Bemisia tabaci in controlled and uncontrolled environments that is associated with plant age, damage intensity, and trichome density. The research was conducted under two conditions: non-sprayed (NS) and sprayed (SP). This study used 50 soybean genotypes arranged in a randomized block design with three replicates. The whitefly population was derived from natural infestations. The results showed that the highest wild population of B. tabaci occurred at 40 days after planting (DAP), i.e., 126.08 adults/plant in the NS environment and 22.57 adults/plant in the SP environment. The peak damage intensity occurred at 50 DAP, 20.71% in the NS environment, and 17.15% in the SP environment. In the NS environment, there were six resistant genotypes (including the resistant control G100H), 25 moderate, and 19 susceptible genotypes. In the SP environment, 19 genotypes were resistant, 22 genotypes were moderate, and nine genotypes were susceptible, respectively. Six soybean genotypes showed consistent resistance to B. tabaci in NS and SP environments. The low density of leaf trichomes in soybean may influence the high resistance to B. tabaci. The resistant genotypes identified in this study could be utilized in breeding programs for B. tabaci resistance.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45463294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-24DOI: 10.14393/bj-v39n0a2023-65615
Laura Vitória Rizzatto, M. D. Di Domênico, Kauê Collares, J. D. de Carli, P. Corazza
This article aims to associate patient-reported pain intensity in the immediate seven days after dental implant surgery with surgical factors, sex and patient age. The sample was composed of 108 patients from a dental school in southern Brazilian, between 2018 and 2020. The variables torque, pre- and postoperative medication, healing of first and second intention, gender, age, number of quadrants, number of implants and type of surgery were related to the outcome pain. Pain was reported every day by the patient until the seventh day after surgery, using a visual analogic scale (VAS). Univariate Poisson regression models were used to assess the relationship among pain and the factors. Rate ratios were obtained with 95% of confidence intervals. Overall pain was reported as moderate/intense (VAS: 3-10) by 30.56% of patients, mild pain (VAS: 1-3) by 55.56%, and no pain symptoms were reported by 13.89% of patients. Individuals which the implants were installed with high torques (50-80 N) showed more pain (p=0.03) compared to patients which the implants were installed with regular torque (30-45 N). The other factors evaluated were not significant. The torque was the most relevant factor related to pain among the evaluated by the study. High torque generates more postoperative pain than lower torque. The factors gender, age, number of operated quadrants, number of installed implants, type of surgery and pre and postoperative analgesic did not interfere in the patient's pain during the first week after surgery.
{"title":"Pain factors related to dental implant surgery: A 7-day observational clinical study","authors":"Laura Vitória Rizzatto, M. D. Di Domênico, Kauê Collares, J. D. de Carli, P. Corazza","doi":"10.14393/bj-v39n0a2023-65615","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-65615","url":null,"abstract":"This article aims to associate patient-reported pain intensity in the immediate seven days after dental implant surgery with surgical factors, sex and patient age. The sample was composed of 108 patients from a dental school in southern Brazilian, between 2018 and 2020. The variables torque, pre- and postoperative medication, healing of first and second intention, gender, age, number of quadrants, number of implants and type of surgery were related to the outcome pain. Pain was reported every day by the patient until the seventh day after surgery, using a visual analogic scale (VAS). Univariate Poisson regression models were used to assess the relationship among pain and the factors. Rate ratios were obtained with 95% of confidence intervals. Overall pain was reported as moderate/intense (VAS: 3-10) by 30.56% of patients, mild pain (VAS: 1-3) by 55.56%, and no pain symptoms were reported by 13.89% of patients. Individuals which the implants were installed with high torques (50-80 N) showed more pain (p=0.03) compared to patients which the implants were installed with regular torque (30-45 N). The other factors evaluated were not significant. The torque was the most relevant factor related to pain among the evaluated by the study. High torque generates more postoperative pain than lower torque. The factors gender, age, number of operated quadrants, number of installed implants, type of surgery and pre and postoperative analgesic did not interfere in the patient's pain during the first week after surgery.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46809268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-24DOI: 10.14393/bj-v39n0a2023-65305
Suhyeon Park, Ji-soo Park, Sang-Kyu Park
Ezetimibe is an approved drug for lowering plasma LDL (low-density lipoprotein) level via inhibition of cholesterol absorption. Derivatives of ezetimibe reduce inflammatory response and oxidative stress. In the present study, we investigated the effect of dietary supplementation with ezetimibe in response to environmental stressors and found that ezetimibe increases resistance to oxidative stress and ultraviolet irradiation. Ezetimibe also significantly extended lifespan accompanying reduced fertility, which is a common trade-off for longevity in C. elegans. Cellular level of reactive oxygen species was increased and the expression of stress-responsive genes, hsp-16.2 and sod-3, was induced by dietary supplementation with ezetimibe, suggesting a hormetic effect on oxidative stress response and lifespan. Ezetimibe also significantly prevented amyloid beta-induced toxicity and completely reversed increased mortality by high-glucose diet. Nuclear localization of DAF-16 required for the prevention of amyloid beta-induced toxicity was enhanced by ezetimibe supplementation. Lifespan assay using known long-lived mutants, age-1, clk-1, and eat-2, revealed that lifespan extension by ezetimibe specifically overlapped with that of eat-2 mutants, which are genetic models of dietary restriction. Effect of ezetimibe on lifespan of worms fed with diluted bacteria suggested that ezetimibe mimics the effect of dietary restriction on lifespan. These findings suggest that ezetimibe exhibits anti-oxidative and anti-aging effects through hormesis and works as a dietary-restriction mimetic on lifespan extension.
{"title":"Ezetimibe increases resistance to oxidative stress and extends lifespan mimicking dietary restriction in Caenorhabditis elegans","authors":"Suhyeon Park, Ji-soo Park, Sang-Kyu Park","doi":"10.14393/bj-v39n0a2023-65305","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-65305","url":null,"abstract":"Ezetimibe is an approved drug for lowering plasma LDL (low-density lipoprotein) level via inhibition of cholesterol absorption. Derivatives of ezetimibe reduce inflammatory response and oxidative stress. In the present study, we investigated the effect of dietary supplementation with ezetimibe in response to environmental stressors and found that ezetimibe increases resistance to oxidative stress and ultraviolet irradiation. Ezetimibe also significantly extended lifespan accompanying reduced fertility, which is a common trade-off for longevity in C. elegans. Cellular level of reactive oxygen species was increased and the expression of stress-responsive genes, hsp-16.2 and sod-3, was induced by dietary supplementation with ezetimibe, suggesting a hormetic effect on oxidative stress response and lifespan. Ezetimibe also significantly prevented amyloid beta-induced toxicity and completely reversed increased mortality by high-glucose diet. Nuclear localization of DAF-16 required for the prevention of amyloid beta-induced toxicity was enhanced by ezetimibe supplementation. Lifespan assay using known long-lived mutants, age-1, clk-1, and eat-2, revealed that lifespan extension by ezetimibe specifically overlapped with that of eat-2 mutants, which are genetic models of dietary restriction. Effect of ezetimibe on lifespan of worms fed with diluted bacteria suggested that ezetimibe mimics the effect of dietary restriction on lifespan. These findings suggest that ezetimibe exhibits anti-oxidative and anti-aging effects through hormesis and works as a dietary-restriction mimetic on lifespan extension.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41747386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-24DOI: 10.14393/bj-v39n0a2023-65634
Tiago Lima DO Nascimento, J. M. R. Barros, G. M. Oliveira, Camila Barbosa Dos Santos, T. V. Voltolini, R. P. Antônio, F. Angelotti
Climate changes can influence the genetic diversity of forage plants, which may contribute to the improvement and development of new species. Therefore, this research aimed to evaluate the influence of temperature increase on the genetic diversity of Macroptilium accessions based on morphoagronomic descriptors. The experiment was carried out in a growth chamber in a 2×16 factorial arrangement (temperature regimes x Macroptilium accessions), with the temperatures consisting of T1 (20–26–33 °C) and T2 (24.8–30.8–37.8 °C) and 16 accessions. Eleven morphoagronomic descriptors allowed estimating the diversity among accessions. The measurements of genetic dissimilarity enabled us to observe the genetic distance between the studied materials, standing out the accessions T1.M3 and T2.S4 as the most divergent (446.01). The morphoagronomic descriptors percentage of leaves and stem diameter were the most efficient for estimating the diversity between access. Genetic variability points to the adaptation of Macroptilium accessions in the climate change scenario. The accessions more divergent can be explored in genetic breeding programs for the species aiming at the expansion of genetic variability as an adaptation mechanism to heat stress.
{"title":"Genetic diversity of Macroptilium accessions considering the increase in air temperature","authors":"Tiago Lima DO Nascimento, J. M. R. Barros, G. M. Oliveira, Camila Barbosa Dos Santos, T. V. Voltolini, R. P. Antônio, F. Angelotti","doi":"10.14393/bj-v39n0a2023-65634","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-65634","url":null,"abstract":"Climate changes can influence the genetic diversity of forage plants, which may contribute to the improvement and development of new species. Therefore, this research aimed to evaluate the influence of temperature increase on the genetic diversity of Macroptilium accessions based on morphoagronomic descriptors. The experiment was carried out in a growth chamber in a 2×16 factorial arrangement (temperature regimes x Macroptilium accessions), with the temperatures consisting of T1 (20–26–33 °C) and T2 (24.8–30.8–37.8 °C) and 16 accessions. Eleven morphoagronomic descriptors allowed estimating the diversity among accessions. The measurements of genetic dissimilarity enabled us to observe the genetic distance between the studied materials, standing out the accessions T1.M3 and T2.S4 as the most divergent (446.01). The morphoagronomic descriptors percentage of leaves and stem diameter were the most efficient for estimating the diversity between access. Genetic variability points to the adaptation of Macroptilium accessions in the climate change scenario. The accessions more divergent can be explored in genetic breeding programs for the species aiming at the expansion of genetic variability as an adaptation mechanism to heat stress.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46335904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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