Pub Date : 2023-02-03DOI: 10.14393/bj-v39n0a2023-63133
Yang Yang, Y. Fu, Qiupu Yang, Chen Li
Tillering onion is a herbaceous plant belonging to the Liliaceae family. We cloned the cDNAs of the actin gene (AcACT, GenBank: MF919598) of tillering onion using rapid amplification of the cDNA ends. The full-length cDNA of AcACT was 1,357 bp long with an open reading frame of 1,131 bp encoding 376 amino acids. The amino acid sequence of AcACT shared > 96% similarity with the amino acid sequences of other ACTs and was found (by means of phylogenetic tree analysis) to be closely related to those of Ananas comosus and Papaver somniferum. AcACT expressions showed no significant differences (p > 0.01) in two cultivars L-SH and L-SY over three growth periods and under suitable conditions, low temperature, and short-day conditions. In addition, AcACT was used as an internal reference gene to analyse the expression of the alliinase gene (AcALL). AcALL expression trends in the roots, stems and leaves were consistent with those of diallyl disulphide and diallyl trisulphide. Thus, AcACT is highly conserved and can be used as a suitable internal reference gene when analysing gene expression in tillering onion.
{"title":"Cloning the full-length CDNA of actin gene and analysing alliinase gene expression in tillering onion","authors":"Yang Yang, Y. Fu, Qiupu Yang, Chen Li","doi":"10.14393/bj-v39n0a2023-63133","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-63133","url":null,"abstract":"Tillering onion is a herbaceous plant belonging to the Liliaceae family. We cloned the cDNAs of the actin gene (AcACT, GenBank: MF919598) of tillering onion using rapid amplification of the cDNA ends. The full-length cDNA of AcACT was 1,357 bp long with an open reading frame of 1,131 bp encoding 376 amino acids. The amino acid sequence of AcACT shared > 96% similarity with the amino acid sequences of other ACTs and was found (by means of phylogenetic tree analysis) to be closely related to those of Ananas comosus and Papaver somniferum. AcACT expressions showed no significant differences (p > 0.01) in two cultivars L-SH and L-SY over three growth periods and under suitable conditions, low temperature, and short-day conditions. In addition, AcACT was used as an internal reference gene to analyse the expression of the alliinase gene (AcALL). AcALL expression trends in the roots, stems and leaves were consistent with those of diallyl disulphide and diallyl trisulphide. Thus, AcACT is highly conserved and can be used as a suitable internal reference gene when analysing gene expression in tillering onion.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43130660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-27DOI: 10.14393/bj-v39n0a2023-62323
Frank Bruno Vieira de Sousa, Á. Cruz, Daniela Francisca Soares, C. Cunha, J. Holzbach
Clitoria guianensis and Ouratea spectabilis, found in the Brazilian Cerrado, are used in folk medicine, despite the few chemical and biological studies reported in the literature. The present study aims to investigate the toxicity and effect of extracts from both species on the microcrustacean Artemia salina, and to determine the chemical composition of the hexane extract of O. spectabilis leaves and the EtOAc fraction of C. guianensis leaves. Kaempferitrin, a flavonoid isolated from of the EtOAc fraction of C. guianensis leaves, was identified by chemical analysis. Analysis of the hexane extract of O. spectabilis leaves using gas chromatography-mass spectrometry (GC-MS) suggested the presence of twenty-five known substances. The Hex, EtOAc, and EtOH crude extracts of C. guianensis leaves exhibited high and moderate toxicity against Artemia salina, with median lethal dose values (LD50) of 43.7, 25.4, and 233.4 mg.L−1, respectively. The acetone extract of O. spectabilis leaves showed moderate toxicity against Artemia salina with an LD50 value of 115.13 mg.L−1.
{"title":"Chemical constituents and in vivo preliminary evaluation of the toxicological activity of Ouratea spectabilis (OCHNACEAE) and Clitoria guianensis (Fabaceae) leaves","authors":"Frank Bruno Vieira de Sousa, Á. Cruz, Daniela Francisca Soares, C. Cunha, J. Holzbach","doi":"10.14393/bj-v39n0a2023-62323","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-62323","url":null,"abstract":"Clitoria guianensis and Ouratea spectabilis, found in the Brazilian Cerrado, are used in folk medicine, despite the few chemical and biological studies reported in the literature. The present study aims to investigate the toxicity and effect of extracts from both species on the microcrustacean Artemia salina, and to determine the chemical composition of the hexane extract of O. spectabilis leaves and the EtOAc fraction of C. guianensis leaves. Kaempferitrin, a flavonoid isolated from of the EtOAc fraction of C. guianensis leaves, was identified by chemical analysis. Analysis of the hexane extract of O. spectabilis leaves using gas chromatography-mass spectrometry (GC-MS) suggested the presence of twenty-five known substances. The Hex, EtOAc, and EtOH crude extracts of C. guianensis leaves exhibited high and moderate toxicity against Artemia salina, with median lethal dose values (LD50) of 43.7, 25.4, and 233.4 mg.L−1, respectively. The acetone extract of O. spectabilis leaves showed moderate toxicity against Artemia salina with an LD50 value of 115.13 mg.L−1.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46336379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-27DOI: 10.14393/bj-v39n0a2023-62426
Aliya Riaz, Sana Ahmad, Ayesha Siddiqui, F. Jabeen, Farah Tariq, Shah Ali Ul Qader
1,4-alpha-D-glucan glucanohydrolase is among the most widely used commercial hydrolytic enzymes acting randomly on the glycosidic linkages of starch resulting in its saccharification and liquefaction. Its applicability in different industries can be improved by enhancing its stability and reusability. Therefore, in the present study attempts have been made to enhance the industrial applicability of 1,4-alpha-D-glucan glucanohydrolase from Bacillus subtilis KIBGE-HAR by adapting immobilization technology. The study developed mechanically stable, enzyme containing gel-frameworks using two support matrices including agar-agar, a natural polysaccharide and polyacrylamide gel, a synthetic organic polymer. These catalytic gel-scaffolds were compared with each other in terms of kinetics and stability of entrapped 1,4-α-D-glucan glucanohydrolase. In case of polyacrylamide gel, Km value for immobilized enzyme increased to 7.95 mg/mL, while immobilization in agar-agar resulted in decreased Km value i.e 0.277 mg/mL as compared to free enzyme. It was found that immobilized enzyme showed maximum activity at 70 °C in both the supports as compared to free enzyme having maximum activity at 60 °C. Immobilized 1,4-α-D-glucan glucanohydrolase exhibited no change in optimal pH 7.0 before and after entrapment in polyacrylamide gel and agar-agar. The enzyme containing gel-scaffold was found suitable for repeated batches of starch liquefaction in industrial processes. Agar-agar entrapped 1,4-α-D-glucanglucanohydrolase was capable to degrade starch up to seven repeated operational cycles whereas polyacrylamide entrapped enzyme conserved its activity up to sixth operational cycle.
1,4- α - d -葡聚糖葡聚糖水解酶是应用最广泛的商业水解酶之一，它随机作用于淀粉的糖苷键，导致淀粉的糖化和液化。通过增强其稳定性和可重用性，可以提高其在不同行业的适用性。因此，本研究尝试采用固定化技术提高枯草芽孢杆菌KIBGE-HAR中1,4- α - d -葡聚糖葡聚糖水解酶的工业适用性。该研究开发了机械稳定、含有酶的凝胶框架，使用两种支撑基质，包括琼脂(一种天然多糖)和聚丙烯酰胺凝胶(一种合成有机聚合物)。对这些催化凝胶支架包埋的1,4-α- d -葡聚糖葡聚糖水解酶的动力学和稳定性进行了比较。在聚丙烯酰胺凝胶中，固定化酶的Km值增加到7.95 mg/mL，而琼脂中固定化酶的Km值比游离酶降低了0.277 mg/mL。结果表明，固定化酶在70℃时活性最高，而游离酶在60℃时活性最高。固定化的1,4-α- d -葡聚糖葡聚糖水解酶在聚丙烯酰胺凝胶和琼脂包埋前后的最佳pH 7.0没有变化。发现含酶凝胶支架适用于工业过程中淀粉的重复批次液化。琼脂包埋的1,4-α- d -葡聚糖水解酶能够降解淀粉长达7个重复的操作循环，而聚丙烯酰胺包埋的酶在第6个操作循环中保持其活性。
{"title":"Fabrication of 1,4-alpha-D-glucan glucanohydrolase holding Gel-Scaffolds using Agar-Agar, a natural polysaccharide and Polyacrylamide, a synthetic organic polymer for continuous liquefaction of starch","authors":"Aliya Riaz, Sana Ahmad, Ayesha Siddiqui, F. Jabeen, Farah Tariq, Shah Ali Ul Qader","doi":"10.14393/bj-v39n0a2023-62426","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-62426","url":null,"abstract":"1,4-alpha-D-glucan glucanohydrolase is among the most widely used commercial hydrolytic enzymes acting randomly on the glycosidic linkages of starch resulting in its saccharification and liquefaction. Its applicability in different industries can be improved by enhancing its stability and reusability. Therefore, in the present study attempts have been made to enhance the industrial applicability of 1,4-alpha-D-glucan glucanohydrolase from Bacillus subtilis KIBGE-HAR by adapting immobilization technology. The study developed mechanically stable, enzyme containing gel-frameworks using two support matrices including agar-agar, a natural polysaccharide and polyacrylamide gel, a synthetic organic polymer. These catalytic gel-scaffolds were compared with each other in terms of kinetics and stability of entrapped 1,4-α-D-glucan glucanohydrolase. In case of polyacrylamide gel, Km value for immobilized enzyme increased to 7.95 mg/mL, while immobilization in agar-agar resulted in decreased Km value i.e 0.277 mg/mL as compared to free enzyme. It was found that immobilized enzyme showed maximum activity at 70 °C in both the supports as compared to free enzyme having maximum activity at 60 °C. Immobilized 1,4-α-D-glucan glucanohydrolase exhibited no change in optimal pH 7.0 before and after entrapment in polyacrylamide gel and agar-agar. The enzyme containing gel-scaffold was found suitable for repeated batches of starch liquefaction in industrial processes. Agar-agar entrapped 1,4-α-D-glucanglucanohydrolase was capable to degrade starch up to seven repeated operational cycles whereas polyacrylamide entrapped enzyme conserved its activity up to sixth operational cycle.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43065158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-27DOI: 10.14393/bj-v39n0a2023-62601
Osléias Ferreira Aguiar, João David Batista Lisboa, Ruy Bessa Lopes, Lucinewton Silva de Moura, Maxwell Barbosa de Santana
Animal welfare regards the quality of life and the environment in which animals live or are exposed. Hence the importance of studies assessing the environmental influence on the biology and behavior of fishes from the Colossoma macropomum species, considering their higher potential for fish-farming and as a test organism in scientific research. This study aimed to assess, in a controlled environment, the behavioral changes expressed by juveniles from the Colossoma macropomum species exposed to different ambient colors or social situations. The results did not show an influence from the different ambient colors or social situations on fish distribution in the water column. The color pattern showed dark tones in black- and blue-colored environments, and light tones in white-colored environments or with the presence of a mirror. The time of locomotor activity/frequency did not change in any of the treatments of exposure to environments with colors and a mirror. Moreover, the fishes remained with folded fins and a straight posture, maintaining a pattern of rhythmic operculum beating in a normal frequency range described for the species. This suggests that animal welfare did not change in any of the situations tested and that there was a pattern of adaptive response to the type of environment.
{"title":"Behavioral assessment of Tambaqui juveniles (Colossoma macropomum) exposed to different ambient colors and social stress","authors":"Osléias Ferreira Aguiar, João David Batista Lisboa, Ruy Bessa Lopes, Lucinewton Silva de Moura, Maxwell Barbosa de Santana","doi":"10.14393/bj-v39n0a2023-62601","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-62601","url":null,"abstract":"Animal welfare regards the quality of life and the environment in which animals live or are exposed. Hence the importance of studies assessing the environmental influence on the biology and behavior of fishes from the Colossoma macropomum species, considering their higher potential for fish-farming and as a test organism in scientific research. This study aimed to assess, in a controlled environment, the behavioral changes expressed by juveniles from the Colossoma macropomum species exposed to different ambient colors or social situations. The results did not show an influence from the different ambient colors or social situations on fish distribution in the water column. The color pattern showed dark tones in black- and blue-colored environments, and light tones in white-colored environments or with the presence of a mirror. The time of locomotor activity/frequency did not change in any of the treatments of exposure to environments with colors and a mirror. Moreover, the fishes remained with folded fins and a straight posture, maintaining a pattern of rhythmic operculum beating in a normal frequency range described for the species. This suggests that animal welfare did not change in any of the situations tested and that there was a pattern of adaptive response to the type of environment.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42499433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-27DOI: 10.14393/bj-v39n0a2023-62708
Lucio Marques Vieira-Souza, Felipe J Aidar, Jymmys Lopes dos Santos, E. Kalinine, Jhennyfer Aline Lima Rodrigues, José Uilien de Oliveira, Jéssica Denielle Matos dos Santos, Márcio Getirana Mota, Waleska dos Santos, Anderson Carlos Marçal
The present study aimed to investigate the effectiveness of high-intensity interval training (HIIT) in the body composition of Wistar rats. The HIIT protocol consisted of high-intensity swimming three times a week for four weeks. There were no differences between groups as to the Lee index. However, the weights of the perigonadal (p=0.001) and retroperitoneal (p=0.026) fats were significantly different between the Control Group (CG, n=10) vs. Trained Group (TG, n=10), respectively. There was also a significant increase in the body weight of the animals in TG (16.43%) and CG (7.19%) at the end of the experiment. These findings suggested that HIIT was not sufficient to improve significantly the body composition of rats.
{"title":"Changes in body composition of Wistar rats: effects of high-intensity interval training","authors":"Lucio Marques Vieira-Souza, Felipe J Aidar, Jymmys Lopes dos Santos, E. Kalinine, Jhennyfer Aline Lima Rodrigues, José Uilien de Oliveira, Jéssica Denielle Matos dos Santos, Márcio Getirana Mota, Waleska dos Santos, Anderson Carlos Marçal","doi":"10.14393/bj-v39n0a2023-62708","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-62708","url":null,"abstract":"The present study aimed to investigate the effectiveness of high-intensity interval training (HIIT) in the body composition of Wistar rats. The HIIT protocol consisted of high-intensity swimming three times a week for four weeks. There were no differences between groups as to the Lee index. However, the weights of the perigonadal (p=0.001) and retroperitoneal (p=0.026) fats were significantly different between the Control Group (CG, n=10) vs. Trained Group (TG, n=10), respectively. There was also a significant increase in the body weight of the animals in TG (16.43%) and CG (7.19%) at the end of the experiment. These findings suggested that HIIT was not sufficient to improve significantly the body composition of rats.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":"1 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41328036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-27DOI: 10.14393/bj-v39n0a2023-60568
N. Gilani, Tahira Maqbool, Muhammad Mohibullah, Ume Habiba, T. Anwar, Huma Qureshi
Lysine is an essential amino acid that is not biologically manufactured in the body. Different chemical methods for lysine production are expensive and give low yields. The present study was conducted with the purpose to evaluate the biochemical production of lysine by different carbon sources using bacterial isolates. Three carbon sources namely glucose, sucrose, and fructose were used to evaluate the biochemical production of lysine by Escherichia coli and Klebsiella spp. isolates. Optimum incubation periods were between 48-96 hours. An extensive amount of lysine was produced by all of these isolates in L6 fermentation medium. Maximum lysine was produced by Klebsiella isolate K1 6.48 g/L after 96 hours of incubation by using glucose as carbon source followed by 6.0 g/L by Klebsiella isolates K3 after 72 hours of incubation when sucrose was used as a carbon source at 37 °C. Highest amount of lysine was produced at 96 hours by Klebsiella isolates in addition to E. coli. From all three carbon sources using Klebsiella isolates and E. coli, glucose showed better lysine production.
{"title":"Screening of lysine production by Escherichia coli and Klebsiella isolates at different carbon sources","authors":"N. Gilani, Tahira Maqbool, Muhammad Mohibullah, Ume Habiba, T. Anwar, Huma Qureshi","doi":"10.14393/bj-v39n0a2023-60568","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-60568","url":null,"abstract":"Lysine is an essential amino acid that is not biologically manufactured in the body. Different chemical methods for lysine production are expensive and give low yields. The present study was conducted with the purpose to evaluate the biochemical production of lysine by different carbon sources using bacterial isolates. Three carbon sources namely glucose, sucrose, and fructose were used to evaluate the biochemical production of lysine by Escherichia coli and Klebsiella spp. isolates. Optimum incubation periods were between 48-96 hours. An extensive amount of lysine was produced by all of these isolates in L6 fermentation medium. Maximum lysine was produced by Klebsiella isolate K1 6.48 g/L after 96 hours of incubation by using glucose as carbon source followed by 6.0 g/L by Klebsiella isolates K3 after 72 hours of incubation when sucrose was used as a carbon source at 37 °C. Highest amount of lysine was produced at 96 hours by Klebsiella isolates in addition to E. coli. From all three carbon sources using Klebsiella isolates and E. coli, glucose showed better lysine production.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48855409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-27DOI: 10.14393/bj-v39n0a2023-62558
Elvis Samuel, D. Wilson, Catherene Tomy, D. J. I. Nallakannu, Siddikuzaman, Aavany Balasubramanian, V. M. Berlin Grace
The extracts of Platycladus orientalis (L.) Franco leaves have shown promising anti-cancer, anti-oxidant and anti-inflammatory potency with the traditional knowledge of healing HPV associated warts. The purpose of this research is to assess the synergistic activity of sorafenib and Platycladus orientalis (L) leaf extraction on cervical cancer cells. The cytotoxicity efficiency of different concentrations of Sorafenib and ethanol extract of Platycladus orientalis (L.) leaves were tested on HeLa cells by MTT and Trypan blue assays. The synergistic effect of the IC50 concentrations of Sorafenib and Platycladus orientalis (L.) on HeLa cell by MTT assay, and mRNA expression levels of tumor suppressor tazarotene-induced gene 3 (TIG3), proliferating cell nuclear antigen (PCNA) gene and apoptosis modulator (Bcl-2) gene by RT-PCR were evaluated with individual treatments. Combination treatment showed a relatively more expression of TIG3 and less expression of Bcl-2 and PCNA was observed. Growth factor-induced MAPKP activation was arrested by compound combination treatment, which and suppression of proliferation-induced apoptosis of cervical cancer cells. Based on the our results, the combination of sorafenib and crude leaf extract from Platycladus orientalis (L.) can effectively suppress cervical cancer cell growth, thereby providing an interesting rationale for further clinical trials and in-vivo studies.
侧柏提取物的研究佛朗哥叶已经显示出抗癌、抗氧化和抗炎的潜力，传统的知识是治疗HPV相关的疣。本研究的目的是评估索拉非尼和侧柏叶提取物对宫颈癌细胞的协同作用。采用MTT法和台锥蓝法检测不同浓度索拉非尼和乙醇提取物对HeLa细胞的细胞毒作用。MTT法检测索拉非尼和侧柏(Platycladus orientalis, L.) IC50浓度对HeLa细胞的协同作用，RT-PCR法检测他扎罗汀诱导的肿瘤抑制基因3 (TIG3)、增殖细胞核抗原(PCNA)基因和凋亡调节剂(Bcl-2)基因mRNA表达水平。联合治疗组TIG3表达较高，Bcl-2和PCNA表达较低。复合治疗可抑制生长因子诱导的MAPKP活化，抑制增殖诱导的宫颈癌细胞凋亡。基于我们的研究结果，索拉非尼与Platycladus orientalis (L.)粗叶提取物联合使用可以有效抑制宫颈癌细胞的生长，从而为进一步的临床试验和体内研究提供了有趣的理论依据。
{"title":"Synergistic effect of sorafenib with Platycladus orientalis (L) leaf extract on cervical cancer","authors":"Elvis Samuel, D. Wilson, Catherene Tomy, D. J. I. Nallakannu, Siddikuzaman, Aavany Balasubramanian, V. M. Berlin Grace","doi":"10.14393/bj-v39n0a2023-62558","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-62558","url":null,"abstract":"The extracts of Platycladus orientalis (L.) Franco leaves have shown promising anti-cancer, anti-oxidant and anti-inflammatory potency with the traditional knowledge of healing HPV associated warts. The purpose of this research is to assess the synergistic activity of sorafenib and Platycladus orientalis (L) leaf extraction on cervical cancer cells. The cytotoxicity efficiency of different concentrations of Sorafenib and ethanol extract of Platycladus orientalis (L.) leaves were tested on HeLa cells by MTT and Trypan blue assays. The synergistic effect of the IC50 concentrations of Sorafenib and Platycladus orientalis (L.) on HeLa cell by MTT assay, and mRNA expression levels of tumor suppressor tazarotene-induced gene 3 (TIG3), proliferating cell nuclear antigen (PCNA) gene and apoptosis modulator (Bcl-2) gene by RT-PCR were evaluated with individual treatments. Combination treatment showed a relatively more expression of TIG3 and less expression of Bcl-2 and PCNA was observed. Growth factor-induced MAPKP activation was arrested by compound combination treatment, which and suppression of proliferation-induced apoptosis of cervical cancer cells. Based on the our results, the combination of sorafenib and crude leaf extract from Platycladus orientalis (L.) can effectively suppress cervical cancer cell growth, thereby providing an interesting rationale for further clinical trials and in-vivo studies.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44750538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-20DOI: 10.14393/bj-v39n0a2023-62191
Andyara Thalissa Forin Paes, A. Barbieri, Andréia Insabralde de Queiroz Cardoso, M. A. Ferreira Júnior
To analyze the knowledge and safety of primary health care professionals in the management of chronic kidney disease in its early stages. Integrative literature review carried out in four steps. The search took place in the following databases: MEDLINE via PubMed, Web of Science, Scopus and BVS. The descriptors used were “Primary Health Care” AND “Kidney Diseases” AND “Health Knowledge, Attitudes, Practice” and the quality of the analysis was checked by means of the Hierarchy of Evidence for Intervention Studies”. Fifteen articles were found, with a predominance of qualitative and descriptive method; the results showed insufficient knowledge of health professionals and interest in the best approach in the initial stage of the disease. Thus, it is inferred that the knowledge and security of primary health care professionals depend on individual factors and institutional initiative for the adoption of clinical guidelines and training. Therefore, prepared professionals and organized care, using protocols, would bring benefits both to patients as to the evolution and outcome of the disease and savings to health services.
分析初级卫生保健专业人员在慢性肾脏疾病早期管理中的知识和安全性。综合文献综述分四步进行。搜索在以下数据库中进行：MEDLINE通过PubMed、Web of Science、Scopus和BVS。所用的描述词是“初级卫生保健”、“肾脏疾病”和“健康知识、态度、实践”，并通过干预研究的证据层次来检查分析的质量。共发现15篇文章，以定性和描述性方法为主；结果显示，在疾病的早期阶段，卫生专业人员的知识和对最佳方法的兴趣不足。因此，可以推断，初级卫生保健专业人员的知识和安全取决于采用临床指南和培训的个人因素和机构主动性。因此，有准备的专业人员和有组织的护理，使用协议，将为患者带来疾病演变和结果方面的好处，并为医疗服务节省开支。
{"title":"Chronic kidney disease intervention in primary health care: integrative review","authors":"Andyara Thalissa Forin Paes, A. Barbieri, Andréia Insabralde de Queiroz Cardoso, M. A. Ferreira Júnior","doi":"10.14393/bj-v39n0a2023-62191","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-62191","url":null,"abstract":"To analyze the knowledge and safety of primary health care professionals in the management of chronic kidney disease in its early stages. Integrative literature review carried out in four steps. The search took place in the following databases: MEDLINE via PubMed, Web of Science, Scopus and BVS. The descriptors used were “Primary Health Care” AND “Kidney Diseases” AND “Health Knowledge, Attitudes, Practice” and the quality of the analysis was checked by means of the Hierarchy of Evidence for Intervention Studies”. Fifteen articles were found, with a predominance of qualitative and descriptive method; the results showed insufficient knowledge of health professionals and interest in the best approach in the initial stage of the disease. Thus, it is inferred that the knowledge and security of primary health care professionals depend on individual factors and institutional initiative for the adoption of clinical guidelines and training. Therefore, prepared professionals and organized care, using protocols, would bring benefits both to patients as to the evolution and outcome of the disease and savings to health services.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42590929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-20DOI: 10.14393/bj-v39n0a2023-62137
Juliana Vianna Pereira, Tamiris Christensen Bueno, F. Herkrath, Mirlena Mansur Dionizio da Silva, Milena Cardia Leme, A. Santos-Silva, M. Lopes
This study aimed to address the subjective oral health measures of caregivers of individuals with autism spectrum disorder (ASD) and Down syndrome. This cross-sectional study included 15 caregivers of individuals with ASD (n = 7) and Down syndrome (n = 8). Sociodemographic data, sense of coherence (SOC) (SOC-13 scale), social support (Social Support Scale [MOS-SSS]), oral health-related quality of life (OHRQoL) (OHIP-14), and self-rated oral health assessed on a 5-point Likert scale were collected. A descriptive analysis was performed in addition to correlation analyses (Spearman correlation coefficient). Most primary caregivers were parents of the individual (86.6%). The age of the caregivers ranged between 40 and 59 years (60%). Most caregivers have had 9 to 11 years of education (53.3%). Monthly family income was less than USD 186,28 for ASD caregivers (57.2%) and between USD 327,56 and USD 931,40 for Down syndrome caregivers (50%). Of the caregivers, 33.3% reported good oral health and 33.3% reported neither good nor bad. The average SOC score, social support and OHRQoL was 48.9, 69.3 and 10.9, respectively. The higher the family income, the better the OHRQoL (rs = -0.62, p = 0.014). SOC was correlated with the score of the emotional support domain (rs = 0.54, p = 0.039). It was concluded that caregivers had a strong SOC and high perceived support. Moreover, caregivers did not report a high impact on OHRQoL A better understanding of the caregivers’ protective and coping factors in caring for individuals with disabilities may better promote their quality of life.
{"title":"Subjective oral health measures in caregivers of patients with autism spectrum disorder and down syndrome: a preliminary study","authors":"Juliana Vianna Pereira, Tamiris Christensen Bueno, F. Herkrath, Mirlena Mansur Dionizio da Silva, Milena Cardia Leme, A. Santos-Silva, M. Lopes","doi":"10.14393/bj-v39n0a2023-62137","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-62137","url":null,"abstract":"This study aimed to address the subjective oral health measures of caregivers of individuals with autism spectrum disorder (ASD) and Down syndrome. This cross-sectional study included 15 caregivers of individuals with ASD (n = 7) and Down syndrome (n = 8). Sociodemographic data, sense of coherence (SOC) (SOC-13 scale), social support (Social Support Scale [MOS-SSS]), oral health-related quality of life (OHRQoL) (OHIP-14), and self-rated oral health assessed on a 5-point Likert scale were collected. A descriptive analysis was performed in addition to correlation analyses (Spearman correlation coefficient). Most primary caregivers were parents of the individual (86.6%). The age of the caregivers ranged between 40 and 59 years (60%). Most caregivers have had 9 to 11 years of education (53.3%). Monthly family income was less than USD 186,28 for ASD caregivers (57.2%) and between USD 327,56 and USD 931,40 for Down syndrome caregivers (50%). Of the caregivers, 33.3% reported good oral health and 33.3% reported neither good nor bad. The average SOC score, social support and OHRQoL was 48.9, 69.3 and 10.9, respectively. The higher the family income, the better the OHRQoL (rs = -0.62, p = 0.014). SOC was correlated with the score of the emotional support domain (rs = 0.54, p = 0.039). It was concluded that caregivers had a strong SOC and high perceived support. Moreover, caregivers did not report a high impact on OHRQoL A better understanding of the caregivers’ protective and coping factors in caring for individuals with disabilities may better promote their quality of life.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49235716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-16DOI: 10.14393/bj-v38n0a2022-54959
Liangliang Xu, Chunhong Xiao, H-Y Cao, Jianfen Yuan, Z. Wu
This study was designed to compare the effects of Jinkui Shenqi and Wuzi Yanzong pill on sperm motility and sperm DNA fragmentation rate in patients with asthenospermia. 130 cases were randomly divided into an observation and control group (n=65). The control group was treated with the Wuzi Yanzong pill while the observation group with the Jinkui Shenqi pill. The sperm motility parameters rate (PR), semen concentration, sperm motility, DFI and α-glucosidase, fructose, seminal plasma zinc (Zn), acid phosphatase (ACP) in seminal plasma biochemistry and other indexes of were observed. The biochemical indexes of seminal plasma of α-glucosidase, fructose, Zn, ACP in two groups were significantly (p<0.05) improved after treatment. Compared with the control group, the indexes of the observation group improved more obviously after treatment. Pearson correlation analysis of DFI and PR indexes in 130 patients before treatment showed that sperm DFI and PR percentage were negatively correlated in asthenospermia patients (r =-0.572, P<0.05). There was no significant difference in DFI, semen concentration, PR, and sperm motility between the two groups before treatment. The DFI, semen concentration, PR and sperm viability of the two groups showed a tendency to improve after treatment, and the effect of the observation group was less significant than that of the control group (p<0.05). Two groups of patients have completed treatment successfully, no adverse events occurred during treatment. Jinkui Shenqi pill can effectively treat asthenospermia, which can effectively improve the effect of sperm motility in patients. It has less adverse reactions, safe and reliable, and is worthy of promotion.
{"title":"Effects of Jinkui Shenqi and Wuzi Yanzong pill on sperm motility and sperm DNA fragmentation rate in asthenospermia","authors":"Liangliang Xu, Chunhong Xiao, H-Y Cao, Jianfen Yuan, Z. Wu","doi":"10.14393/bj-v38n0a2022-54959","DOIUrl":"https://doi.org/10.14393/bj-v38n0a2022-54959","url":null,"abstract":"This study was designed to compare the effects of Jinkui Shenqi and Wuzi Yanzong pill on sperm motility and sperm DNA fragmentation rate in patients with asthenospermia. 130 cases were randomly divided into an observation and control group (n=65). The control group was treated with the Wuzi Yanzong pill while the observation group with the Jinkui Shenqi pill. The sperm motility parameters rate (PR), semen concentration, sperm motility, DFI and α-glucosidase, fructose, seminal plasma zinc (Zn), acid phosphatase (ACP) in seminal plasma biochemistry and other indexes of were observed. The biochemical indexes of seminal plasma of α-glucosidase, fructose, Zn, ACP in two groups were significantly (p<0.05) improved after treatment. Compared with the control group, the indexes of the observation group improved more obviously after treatment. Pearson correlation analysis of DFI and PR indexes in 130 patients before treatment showed that sperm DFI and PR percentage were negatively correlated in asthenospermia patients (r =-0.572, P<0.05). There was no significant difference in DFI, semen concentration, PR, and sperm motility between the two groups before treatment. The DFI, semen concentration, PR and sperm viability of the two groups showed a tendency to improve after treatment, and the effect of the observation group was less significant than that of the control group (p<0.05). Two groups of patients have completed treatment successfully, no adverse events occurred during treatment. Jinkui Shenqi pill can effectively treat asthenospermia, which can effectively improve the effect of sperm motility in patients. It has less adverse reactions, safe and reliable, and is worthy of promotion.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2022-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45623381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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