Pub Date : 2023-08-18DOI: 10.14393/bj-v39n0a2023-66721
Lorena Bezerra de Medeiros, Antonio Maricélio Borges de Souza, G. R. Vieira, Kássia Barros Ferreira, T. Campos, K. F. L. Pivetta, E. Rigobelo
The demand for sustainable agricultural production systems is increasing, and using growth-promoting microorganisms in plants has stood out because it decreases or even replaces chemical fertilizer use, reducing production costs. This study aimed to evaluate the response of some microorganisms applied to the seedlings of primary orchids cultivated in Brazil (Phalaenopsis sp. 'Taisuco Swan', Cymbidium atropurpureum, and Dendrobium secundum). The experimental design was completely randomized. There were seven treatments (absence of microorganisms – control, Trichoderma sp. in sodium alginate, Trichoderma sp. in clay, Trichoderma sp. in sodium alginate and clay, Trichoderma sp. in a liquid medium, Azospirillum brasilense + Bacillus subtilis in a liquid medium, and Bacillus pumilus in a liquid medium), four replications, and three plants per plot. The seedlings were grown in a greenhouse and evaluated 190 days after microorganism inoculation. The evaluation of morpho-physiological characteristics differed according to the particularities of each genus. The Bacillus pumilus and Azospirillum brasilense + Bacillus subtilis rhizobacteria in a liquid medium for Phalaenopsis sp. 'Taisuco Swan' and the Trichoderma sp. fungus in a liquid medium for Cymbidium atropurpureum increased seedling growth and development. Azospirillum brasilense + Bacillus subtilis in a liquid medium for the Dendrobium secundum orchid promoted more root biomass. Using beneficial microorganisms in orchid cultivation is promising, and seedling growth and development depend on their inoculation and the morpho-physiological characteristics of each plant.
{"title":"Growth-promoting microorganisms in the development of orchid seedlings of Phalaenopsis, Cymbidium, and Dendrobium genera","authors":"Lorena Bezerra de Medeiros, Antonio Maricélio Borges de Souza, G. R. Vieira, Kássia Barros Ferreira, T. Campos, K. F. L. Pivetta, E. Rigobelo","doi":"10.14393/bj-v39n0a2023-66721","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-66721","url":null,"abstract":"The demand for sustainable agricultural production systems is increasing, and using growth-promoting microorganisms in plants has stood out because it decreases or even replaces chemical fertilizer use, reducing production costs. This study aimed to evaluate the response of some microorganisms applied to the seedlings of primary orchids cultivated in Brazil (Phalaenopsis sp. 'Taisuco Swan', Cymbidium atropurpureum, and Dendrobium secundum). The experimental design was completely randomized. There were seven treatments (absence of microorganisms – control, Trichoderma sp. in sodium alginate, Trichoderma sp. in clay, Trichoderma sp. in sodium alginate and clay, Trichoderma sp. in a liquid medium, Azospirillum brasilense + Bacillus subtilis in a liquid medium, and Bacillus pumilus in a liquid medium), four replications, and three plants per plot. The seedlings were grown in a greenhouse and evaluated 190 days after microorganism inoculation. The evaluation of morpho-physiological characteristics differed according to the particularities of each genus. The Bacillus pumilus and Azospirillum brasilense + Bacillus subtilis rhizobacteria in a liquid medium for Phalaenopsis sp. 'Taisuco Swan' and the Trichoderma sp. fungus in a liquid medium for Cymbidium atropurpureum increased seedling growth and development. Azospirillum brasilense + Bacillus subtilis in a liquid medium for the Dendrobium secundum orchid promoted more root biomass. Using beneficial microorganisms in orchid cultivation is promising, and seedling growth and development depend on their inoculation and the morpho-physiological characteristics of each plant.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43232090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-18DOI: 10.14393/bj-v39n0a2023-68631
L. Q. S. Lopes, Pedro Henrique Fortes Guerim, Roberto Christ Vianna Santos, Flávia Kolling Marquezan, P. K. Marquezan
Microorganisms such as Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus are frequently isolated in samples of urinary, blood, intestinal, and respiratory infections, among others. These bacteria are also associated with microbial biofilm formation. E. coli, P. aeruginosa, and S. aureus biofilm infections are particularly hard to manage and often associated with nosocomial problems. This study investigated the influence of different culture media on E. coli, P. aeruginosa, and S. aureus biofilm formation. Bacterial performance was evaluated in brain heart infusion broth, Mueller-Hinton broth, or tryptic soy broth, with or without supplementing with different glucose levels (1-5%). The study quantified biofilm biomass and the count of viable biofilm colonies. This is the first study that compares the biofilm formation of E. coli, P. aeruginosa and S. aureus in polystyrene using different culture media and with different glucose concentrations. The most robust growth of E. coli, P. aeruginosa, and S. aureus occurred in brain heart infusion broth supplemented with 5% glucose, Mueller-Hinton broth without glucose, and tryptic soy broth with 2% glucose, respectively. Our data demonstrate that behavioral and morphological characteristics of each bacterium require a specific broth to enhance the growth of these microorganisms. These findings will contribute to future tests for therapeutic alternatives with anti-biofilm potential.
{"title":"The influence of different culture media on Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus biofilm formation","authors":"L. Q. S. Lopes, Pedro Henrique Fortes Guerim, Roberto Christ Vianna Santos, Flávia Kolling Marquezan, P. K. Marquezan","doi":"10.14393/bj-v39n0a2023-68631","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-68631","url":null,"abstract":"Microorganisms such as Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus are frequently isolated in samples of urinary, blood, intestinal, and respiratory infections, among others. These bacteria are also associated with microbial biofilm formation. E. coli, P. aeruginosa, and S. aureus biofilm infections are particularly hard to manage and often associated with nosocomial problems. This study investigated the influence of different culture media on E. coli, P. aeruginosa, and S. aureus biofilm formation. Bacterial performance was evaluated in brain heart infusion broth, Mueller-Hinton broth, or tryptic soy broth, with or without supplementing with different glucose levels (1-5%). The study quantified biofilm biomass and the count of viable biofilm colonies. This is the first study that compares the biofilm formation of E. coli, P. aeruginosa and S. aureus in polystyrene using different culture media and with different glucose concentrations. The most robust growth of E. coli, P. aeruginosa, and S. aureus occurred in brain heart infusion broth supplemented with 5% glucose, Mueller-Hinton broth without glucose, and tryptic soy broth with 2% glucose, respectively. Our data demonstrate that behavioral and morphological characteristics of each bacterium require a specific broth to enhance the growth of these microorganisms. These findings will contribute to future tests for therapeutic alternatives with anti-biofilm potential.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43342203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-18DOI: 10.14393/bj-v39n0a2023-68570
N. Z. El-alfy, M. Mahmoud, Hend M. AbdElfatah, A. Emam
Gluten is a protein commonly found in daily diets in the form of wheat, barley, rye, and other grains. It serves as the structural component in flour, providing the binding qualities that maintain the shape and texture of food items. This study aimed to investigate the genotoxic and cytotoxic effects of gluten on bone marrow chromosomes and DNA of male albino mice. The animals were divided into four groups: a control group, a negative control group that received an oral dose of 0.02M glacial acetic acid, and two groups that were treated with gluten dissolved in 0.02M glacial acetic acid at doses of 1.5 g/kg and 3.0 g/kg body weight. The treated animals received oral doses with non-consecutively three times a week for a period of four weeks. The study evaluated chromosomal aberrations in the bone marrow, micronucleus test, and DNA damage using the comet assay. The results of the study showed that treatment with 1.5 and 3.0g/kg body weight of gluten induced chromosomal aberrations and damage in DNA content, with an increase in the severity of effects at a higher dose of gluten. The chromosomal aberrations seen included deletion, fragment, centromeric attenuation, centric fusion, ring formation, end to end association, chromosomal gap, beaded chromosomes, and polyploidy. The micronucleus test revealed toxicity in the bone marrow, as shown by appearance of micronuclei in polychromatic erythrocytes and a reduction in the ratio of polychromatic erythrocytes. The comet assay showed a significant increase of DNA damage in the tail length of the comet cells. This study concluded that the treatment with gluten has detrimental effects on the bone marrow chromosomes and DNA of mice, as demonstrated by the increased chromosomal aberrations, micronuclei, and DNA damage observed in the treated mice. So, the use of gluten should be within an acceptable and safe range.
{"title":"Evaluation of Genotoxic and Cytotoxic Effects of Gluten in Male Albino Mice","authors":"N. Z. El-alfy, M. Mahmoud, Hend M. AbdElfatah, A. Emam","doi":"10.14393/bj-v39n0a2023-68570","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-68570","url":null,"abstract":"Gluten is a protein commonly found in daily diets in the form of wheat, barley, rye, and other grains. It serves as the structural component in flour, providing the binding qualities that maintain the shape and texture of food items. This study aimed to investigate the genotoxic and cytotoxic effects of gluten on bone marrow chromosomes and DNA of male albino mice. The animals were divided into four groups: a control group, a negative control group that received an oral dose of 0.02M glacial acetic acid, and two groups that were treated with gluten dissolved in 0.02M glacial acetic acid at doses of 1.5 g/kg and 3.0 g/kg body weight. The treated animals received oral doses with non-consecutively three times a week for a period of four weeks. The study evaluated chromosomal aberrations in the bone marrow, micronucleus test, and DNA damage using the comet assay. The results of the study showed that treatment with 1.5 and 3.0g/kg body weight of gluten induced chromosomal aberrations and damage in DNA content, with an increase in the severity of effects at a higher dose of gluten. The chromosomal aberrations seen included deletion, fragment, centromeric attenuation, centric fusion, ring formation, end to end association, chromosomal gap, beaded chromosomes, and polyploidy. The micronucleus test revealed toxicity in the bone marrow, as shown by appearance of micronuclei in polychromatic erythrocytes and a reduction in the ratio of polychromatic erythrocytes. The comet assay showed a significant increase of DNA damage in the tail length of the comet cells. This study concluded that the treatment with gluten has detrimental effects on the bone marrow chromosomes and DNA of mice, as demonstrated by the increased chromosomal aberrations, micronuclei, and DNA damage observed in the treated mice. So, the use of gluten should be within an acceptable and safe range.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48199765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-23DOI: 10.14393/bj-v39n0a2023-67617
Jiulia Tereza Marques de Castro, Rafael Barreto, E. C. Alvarenga, R. A. Costa
Wound healing remains a clinical problem, with cases of atrophic, hypertrophic, or keloid scars. Three-dimensional scaffolds have been used to restore skin function, facilitating cell migration, adhesion, and proliferation. Collagen is the most common, presenting low antigenicity, decreased inflammation, and replacement by autologous tissue. It is used as sheets/films, sponges, membranes, sprays, and hydrogels of various origins. This integrative literature review aimed to evaluate the application of unassociated collagen scaffolds for skin wound healing and compare them to collagen associations with nanomaterials and polymers. Properties such as applications in humans and other unconventional models cause burns, partial and full-thickness wounds, and others. Scaffold, biomaterials, collagen, wound, injury, repair, and healing were among the descriptors. We found 3,098 articles published between 1995 and 2022 (Mendeley platform), including clinical/experimental trials. After exclusion, 26 studies were identified and analyzed. Autologous and heterologous collagens are the most used in the clinic and favor wound closure by improving re-epithelialization and reducing inflammation but may present challenges in aesthetic acceptance and loss of repair function in the wound site. Furthermore, collagen integration with other nanomaterials improved wound repair and experimental models.
{"title":"Efficacy of collagen-only scaffolds compared to polymer-associated collagen and nanomaterials in skin wound repair – a review","authors":"Jiulia Tereza Marques de Castro, Rafael Barreto, E. C. Alvarenga, R. A. Costa","doi":"10.14393/bj-v39n0a2023-67617","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-67617","url":null,"abstract":"Wound healing remains a clinical problem, with cases of atrophic, hypertrophic, or keloid scars. Three-dimensional scaffolds have been used to restore skin function, facilitating cell migration, adhesion, and proliferation. Collagen is the most common, presenting low antigenicity, decreased inflammation, and replacement by autologous tissue. It is used as sheets/films, sponges, membranes, sprays, and hydrogels of various origins. This integrative literature review aimed to evaluate the application of unassociated collagen scaffolds for skin wound healing and compare them to collagen associations with nanomaterials and polymers. Properties such as applications in humans and other unconventional models cause burns, partial and full-thickness wounds, and others. Scaffold, biomaterials, collagen, wound, injury, repair, and healing were among the descriptors. We found 3,098 articles published between 1995 and 2022 (Mendeley platform), including clinical/experimental trials. After exclusion, 26 studies were identified and analyzed. Autologous and heterologous collagens are the most used in the clinic and favor wound closure by improving re-epithelialization and reducing inflammation but may present challenges in aesthetic acceptance and loss of repair function in the wound site. Furthermore, collagen integration with other nanomaterials improved wound repair and experimental models.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46803616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-23DOI: 10.14393/bj-v39n0a2023-66926
S. Leite, Nicole Silva Gomes, Lucas Alves B. dos Santos, Ana Teresa P. Dell’Isola, B. Leite
As environmental concerns and regulatory requirements increase over time, new alternatives for swine manure disposal emerge. Among them, anaerobic biodigestion is a relevant technology because it reduces the organic load of wastewater before its final disposal and provides economic benefits to farmers with biogas and biofertilizer production. Efficiently managing the anaerobic biodigestion process remains a challenge in developing countries, mainly due to the lack of information from swine meat producers to deal with the complexity of this system. A risk analysis can represent a promising tool for farm assistance because it provides a process overview. Hence, this study reports the results of a process mapping in a swine meat production farm in Minas Gerais, Brazil. This mapping was performed while monitoring biodigestion operational parameters and allowed the identification of the primary causes of process failures and potential environmental impacts. The results showed that anaerobic digestion promotes a relevant environmental gain. However, the need for improved process monitoring, investments in environmental assessment equipment, and technical training for producers also stood out as an improvement opportunity.
{"title":"Swine meat production integrated with energy cogeneration: challenges and opportunities in using anaerobic biodigestion","authors":"S. Leite, Nicole Silva Gomes, Lucas Alves B. dos Santos, Ana Teresa P. Dell’Isola, B. Leite","doi":"10.14393/bj-v39n0a2023-66926","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-66926","url":null,"abstract":"As environmental concerns and regulatory requirements increase over time, new alternatives for swine manure disposal emerge. Among them, anaerobic biodigestion is a relevant technology because it reduces the organic load of wastewater before its final disposal and provides economic benefits to farmers with biogas and biofertilizer production. Efficiently managing the anaerobic biodigestion process remains a challenge in developing countries, mainly due to the lack of information from swine meat producers to deal with the complexity of this system. A risk analysis can represent a promising tool for farm assistance because it provides a process overview. Hence, this study reports the results of a process mapping in a swine meat production farm in Minas Gerais, Brazil. This mapping was performed while monitoring biodigestion operational parameters and allowed the identification of the primary causes of process failures and potential environmental impacts. The results showed that anaerobic digestion promotes a relevant environmental gain. However, the need for improved process monitoring, investments in environmental assessment equipment, and technical training for producers also stood out as an improvement opportunity.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46682688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-09DOI: 10.14393/bj-v39n0a2023-65777
Sabrina Toledo de Araujo, S. Campos, Andréa Yuri Takitani, J. R. Pires, Aline Moreira de Souza, N. Almosny
Hemoparasitism is known to vary according to the presence of vectors and hosts, and hematological examination can be a useful tool for early diagnosis in avian medicine, contributing to monitoring and conservation of bird populations. In this context, we aimed to analyze the clinical and hematological aspects of free-ranging owls seized or rescued from the Atlantic Forest biome of Rio de Janeiro, Brazil. The animals, with or without clinical signs of diseases, were studied regardless of their age, sex, and physical status. Blood samples were collected and complete blood counts were carried out. Five different species were obtained in this study. These species were Megascops choliba (50%), Asio clamator (18.75%), Athene cunicularia (18.75%), Pulsatrix koeniswaldiana (6.25%), and Strix huhula (6.25%). Orphaned birds and traumatic injuries were the causes of admission to the wildlife rehabilitation facility. The search for hemoparasites revealed infection by protozoa of the genera Haemoproteus, Plasmodium, and Trypanosoma. We concluded that hematological findings in seized or rescued owls predominantly suggested the presence of stress or inflammation resulting from post-traumatic situations. The causes of admission were mostly due to human interference, which demonstrates to have a negative impact on the health aspect of owl populations in urban centers, such as Rio de Janeiro. Blood protozoa are circulating among the different populations of owls and although infection by Haemoproteus spp. and Trypanosoma spp. seems to trigger a low pathogenic potential, Plasmodium infection may indicate unfavorable prognosis, endangering wild species.
{"title":"Analysis of clinical and hematological characteristics and search for hemoparasites in owls (Strigiformes: Strigidae) seized or rescued from the Atlantic Forest biome of the Rio de Janeiro (southeastern Brazil)","authors":"Sabrina Toledo de Araujo, S. Campos, Andréa Yuri Takitani, J. R. Pires, Aline Moreira de Souza, N. Almosny","doi":"10.14393/bj-v39n0a2023-65777","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-65777","url":null,"abstract":"Hemoparasitism is known to vary according to the presence of vectors and hosts, and hematological examination can be a useful tool for early diagnosis in avian medicine, contributing to monitoring and conservation of bird populations. In this context, we aimed to analyze the clinical and hematological aspects of free-ranging owls seized or rescued from the Atlantic Forest biome of Rio de Janeiro, Brazil. The animals, with or without clinical signs of diseases, were studied regardless of their age, sex, and physical status. Blood samples were collected and complete blood counts were carried out. Five different species were obtained in this study. These species were Megascops choliba (50%), Asio clamator (18.75%), Athene cunicularia (18.75%), Pulsatrix koeniswaldiana (6.25%), and Strix huhula (6.25%). Orphaned birds and traumatic injuries were the causes of admission to the wildlife rehabilitation facility. The search for hemoparasites revealed infection by protozoa of the genera Haemoproteus, Plasmodium, and Trypanosoma. We concluded that hematological findings in seized or rescued owls predominantly suggested the presence of stress or inflammation resulting from post-traumatic situations. The causes of admission were mostly due to human interference, which demonstrates to have a negative impact on the health aspect of owl populations in urban centers, such as Rio de Janeiro. Blood protozoa are circulating among the different populations of owls and although infection by Haemoproteus spp. and Trypanosoma spp. seems to trigger a low pathogenic potential, Plasmodium infection may indicate unfavorable prognosis, endangering wild species.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41402530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-09DOI: 10.14393/bj-v39n0a2023-67282
José Geraldo Da Silva, E. P. B. Ferreira, A. Nascente, Pedro Henrique Lopes Sarmento, Matheus Messias
The positioning of pods in common bean directly affects grain losses in mechanized harvesting. However, only few studies have assessed facttors that can affect pods positioning. The objective of this work was to determine the effect of plant density, nitrogen fertilization, and fertilization depth on the distribution of pods of the common bean. The field experiments were carried out in two cropping seasons, 2017 and 2018, during the winter period in the Cerrado region. The experimental design was randomized blocks in a 4x2 factorial scheme, with four replications. The treatments consisted of the combination of four sowing densities (5, 10, 15, and 20 plants m-1) with two depths of fertilizer application (6 and 12 cm). The results allowed inferring that the depth of the fertilization does not affect the distribution of pods in the common bean. Plant density does not affect common bean grain yield. More than a quarter of the common bean pods of the BRS FC104 are positioned close to the ground, below 100 mm, in the area where harvester machines operate. Nitrogen fertilization and plant density affect the distribution of pods in common bean plants. At higher doses of N (90 kg ha-1), plant density should be increased. On the other hand, at lower doses (45 kg ha-1), plant density must be reduced. It is concluded that the sowing density can be an efficient strategy to provide the highest positioning of pods in the upper part of the common bean plants, reducing harvest loss.
普通大豆荚的定位直接影响机械化收割的粮食损失。然而，只有少数研究评估了可能影响吊舱定位的因素。本研究的目的是确定植物密度、氮肥和施肥深度对蚕豆荚分布的影响。田间试验在塞拉多地区冬季的2017年和2018年两个种植季节进行。实验设计采用4x2析因方案随机分组，四次重复。处理包括四种播种密度（5、10、15和20株m-1）与两种施肥深度（6和12厘米）的组合。结果表明，施肥深度不影响蚕豆荚的分布。植株密度不影响普通豆的产量。BRS FC104超过四分之一的普通豆荚位于靠近地面的地方，低于100毫米，位于收割机操作的区域。施氮量和密度对普通豆类植物豆荚的分布有影响。在较高剂量的N（90 kg ha-1）下，应增加植物密度。另一方面，在较低剂量（45kg ha-1）下，必须降低植物密度。结果表明，播种密度是一种有效的策略，可以在普通豆类植物的上部提供最高的豆荚位置，减少收获损失。
{"title":"Pod positioning and grain yield of common bean as affected by sowing density, nitrogen fertilization and fertilization depth","authors":"José Geraldo Da Silva, E. P. B. Ferreira, A. Nascente, Pedro Henrique Lopes Sarmento, Matheus Messias","doi":"10.14393/bj-v39n0a2023-67282","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-67282","url":null,"abstract":"The positioning of pods in common bean directly affects grain losses in mechanized harvesting. However, only few studies have assessed facttors that can affect pods positioning. The objective of this work was to determine the effect of plant density, nitrogen fertilization, and fertilization depth on the distribution of pods of the common bean. The field experiments were carried out in two cropping seasons, 2017 and 2018, during the winter period in the Cerrado region. The experimental design was randomized blocks in a 4x2 factorial scheme, with four replications. The treatments consisted of the combination of four sowing densities (5, 10, 15, and 20 plants m-1) with two depths of fertilizer application (6 and 12 cm). The results allowed inferring that the depth of the fertilization does not affect the distribution of pods in the common bean. Plant density does not affect common bean grain yield. More than a quarter of the common bean pods of the BRS FC104 are positioned close to the ground, below 100 mm, in the area where harvester machines operate. Nitrogen fertilization and plant density affect the distribution of pods in common bean plants. At higher doses of N (90 kg ha-1), plant density should be increased. On the other hand, at lower doses (45 kg ha-1), plant density must be reduced. It is concluded that the sowing density can be an efficient strategy to provide the highest positioning of pods in the upper part of the common bean plants, reducing harvest loss.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49119789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jian-Pi-Yi-Shen (JPYS) decoction is a traditional Chinese herbal formula. The present study aimed to investigate the effect of JPYS drug-containing serum on the proliferation and activity of mouse osteoblasts. Sprague‒Dawley rats were fed JPYS decoction, calcium supplement, or normal saline for 6 weeks, and the serum was collected. Mouse osteoblasts were treated with JPYS drug-containing serum, calcium supplement serum, or blank control serum. Cell proliferation was assayed using thiazolyl blue tetrazolium bromide. Levels of alkaline phosphatase, nitric oxide, and nitric oxide synthase in the culture medium were measured. The JPYS drug-containing serum significantly improved the proliferation of osteoblasts compared to the blank control serum and the calcium supplement serum. It also significantly increased the levels of alkaline phosphatase in the culture medium compared to the blank control serum and the calcium supplement serum. Treatment with JPYS drug-containing serum for 48 h and 72 h significantly increased the nitric oxide (NO) concentration in the culture medium compared to the blank control serum. The NOS activity of the osteoblasts was significantly increased by JPYS drug-containing serum compared to blank control serum and calcium supplement serum. All these results were enhanced that the JPYS decoction promotes the proliferation and activity of mouse osteoblasts. These effects may be the underlying mechanisms of JPYS decoction in treating osteoporosis.
{"title":"Jian-Pi-Yi-Shen drug-containing serum promotes proliferation and activity of mouse osteoblasts","authors":"Yu Cai, Xiaochen Liu, Liang Ma, Wen-Ting Lv, Weiguo Wang, Xingcai Zhang","doi":"10.14393/bj-v39n0a2023-64818","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-64818","url":null,"abstract":"Jian-Pi-Yi-Shen (JPYS) decoction is a traditional Chinese herbal formula. The present study aimed to investigate the effect of JPYS drug-containing serum on the proliferation and activity of mouse osteoblasts. Sprague‒Dawley rats were fed JPYS decoction, calcium supplement, or normal saline for 6 weeks, and the serum was collected. Mouse osteoblasts were treated with JPYS drug-containing serum, calcium supplement serum, or blank control serum. Cell proliferation was assayed using thiazolyl blue tetrazolium bromide. Levels of alkaline phosphatase, nitric oxide, and nitric oxide synthase in the culture medium were measured. The JPYS drug-containing serum significantly improved the proliferation of osteoblasts compared to the blank control serum and the calcium supplement serum. It also significantly increased the levels of alkaline phosphatase in the culture medium compared to the blank control serum and the calcium supplement serum. Treatment with JPYS drug-containing serum for 48 h and 72 h significantly increased the nitric oxide (NO) concentration in the culture medium compared to the blank control serum. The NOS activity of the osteoblasts was significantly increased by JPYS drug-containing serum compared to blank control serum and calcium supplement serum. All these results were enhanced that the JPYS decoction promotes the proliferation and activity of mouse osteoblasts. These effects may be the underlying mechanisms of JPYS decoction in treating osteoporosis.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43406231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-15DOI: 10.14393/bj-v39n0a2023-66743
L. E. M. Deef
Order Rodentia contains approximately half of all living mammalian species. Most of the living species diversity within rodents is within five monophyletic radiations: Hystricognathi, Sciuridae, Geomyoidea, Dipodidae, and Muroidea. The samples collected across different areas of Egypt were analyzed by means of mitochondrial cytochrome b gene sequence analysis to ascertain the true taxonomic status between the studied species. For phylogenetic analysis, the cytochrome b sequences from this study were combined with previously published data. This study reveals that all species were divided into two main clades, the first for the Gerbillinae taxon and the second for the other studied genera in the molecular phylogenetic trees and demonstrates that Gerbillus species form a sister group with Dipodillus species. The phylogenetic relationships specified for the murine genera. Clustering was obtained between Mus and Rattus as well as in all Gerbillinae species. This study provides the first insight into the genetic diversity of some rodents in Egypt.
{"title":"Nucleotide sequences of mitochondrial cytochrome b gene for phylogeny of some species of Rodentia","authors":"L. E. M. Deef","doi":"10.14393/bj-v39n0a2023-66743","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-66743","url":null,"abstract":"Order Rodentia contains approximately half of all living mammalian species. Most of the living species diversity within rodents is within five monophyletic radiations: Hystricognathi, Sciuridae, Geomyoidea, Dipodidae, and Muroidea. The samples collected across different areas of Egypt were analyzed by means of mitochondrial cytochrome b gene sequence analysis to ascertain the true taxonomic status between the studied species. For phylogenetic analysis, the cytochrome b sequences from this study were combined with previously published data. This study reveals that all species were divided into two main clades, the first for the Gerbillinae taxon and the second for the other studied genera in the molecular phylogenetic trees and demonstrates that Gerbillus species form a sister group with Dipodillus species. The phylogenetic relationships specified for the murine genera. Clustering was obtained between Mus and Rattus as well as in all Gerbillinae species. This study provides the first insight into the genetic diversity of some rodents in Egypt.\u0000 ","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46639354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-05DOI: 10.14393/bj-v39n0a2023-65423
F. P. D. M. Silveira, W. D. A. R. Lopes, P. R. Oliveira, M. G. D. Santos, Alex Lima Monteiro, G. L. Santos, Silvana Fraga Silva, Ê. G. Souza, Lindomar Maria da Silveira, A. P. B. Júnior
We have evaluated the agronomic performance of table cassava cultivars fertilized with phosphorus doses in the Brazilian Semiarid Region. Two agricultural crops were grown at the Rafael Fernandes Experimental Farm, Mossoró, RN, from June/2018 to April/2019 and from June/2019 to April/2020. The experimental design was in randomized blocks, arranged in subdivided plots, with four replications. In the plots, doses of phosphorus were applied (0, 60, 120, 180 and 240 kg P2O5 ha-1), and in the subplots, the table cassava cultivars (Água Morna, BRS Gema de Ovo, Recife and Venâncio). The following were evaluated: dry matter of leaf, stem, and commercial root; harvest index; commercial root number; commercial productivity and aerial part productivity. The cultivars used had high root and aerial part productivities indicating that their irrigated cultivation is appropriate under the conditions of the Semiarid region of Rio Grande do Norte. The cultivars Água Morna, BRS Gema de Ovo and Recife are more efficient in the use of phosphorus, obtaining high productivity even in the absence of phosphate fertilization. The cultivar Venâncio is more responsive to phosphate fertilization, as it needs an input of this nutrient to increase its productivity.
我们评估了巴西半干旱地区施用磷剂量的木薯品种的农艺性能。2018年6月至2019年4月以及2019年6月和2020年4月，RN州莫索罗的Rafael Fernandes实验农场种植了两种农作物。实验设计采用随机区组，分为四个重复区。在地块中，施用了一定剂量的磷（0、60、120、180和240 kg P2O5 ha-1），在子地块中，则施用了木薯品种（Água Morna、BRS Gema de Ovo、Recife和Venâncio）。对叶、茎和商品根的干物质进行了评价；收获指数；商业根数；商业生产力和航空部件生产力。所用的品种具有较高的根系和地上部生产力，表明其灌溉栽培在北里奥格兰德州半干旱地区的条件下是合适的。品种Água Morna、BRS Gema de Ovo和Recife在磷的利用方面更有效，即使在没有磷肥的情况下也能获得高产。品种Venâncio对磷肥的反应更大，因为它需要这种营养素的输入来提高生产力。
{"title":"Agronomic performance of cassava fertilized with phosphorus doses","authors":"F. P. D. M. Silveira, W. D. A. R. Lopes, P. R. Oliveira, M. G. D. Santos, Alex Lima Monteiro, G. L. Santos, Silvana Fraga Silva, Ê. G. Souza, Lindomar Maria da Silveira, A. P. B. Júnior","doi":"10.14393/bj-v39n0a2023-65423","DOIUrl":"https://doi.org/10.14393/bj-v39n0a2023-65423","url":null,"abstract":"We have evaluated the agronomic performance of table cassava cultivars fertilized with phosphorus doses in the Brazilian Semiarid Region. Two agricultural crops were grown at the Rafael Fernandes Experimental Farm, Mossoró, RN, from June/2018 to April/2019 and from June/2019 to April/2020. The experimental design was in randomized blocks, arranged in subdivided plots, with four replications. In the plots, doses of phosphorus were applied (0, 60, 120, 180 and 240 kg P2O5 ha-1), and in the subplots, the table cassava cultivars (Água Morna, BRS Gema de Ovo, Recife and Venâncio). The following were evaluated: dry matter of leaf, stem, and commercial root; harvest index; commercial root number; commercial productivity and aerial part productivity. The cultivars used had high root and aerial part productivities indicating that their irrigated cultivation is appropriate under the conditions of the Semiarid region of Rio Grande do Norte. The cultivars Água Morna, BRS Gema de Ovo and Recife are more efficient in the use of phosphorus, obtaining high productivity even in the absence of phosphate fertilization. The cultivar Venâncio is more responsive to phosphate fertilization, as it needs an input of this nutrient to increase its productivity.","PeriodicalId":8951,"journal":{"name":"Bioscience Journal","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48893944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：