Pub Date : 2026-01-08DOI: 10.1186/s12879-025-12459-0
Diego U Vazquez-Omana, Paula Beltran-Reyes, Jorge E Alonso-Montoya, Nayeli E Avalos-Celis, Edgar D Centeno-Matus, Sandra Rajme-Lopez, Karla M Tamez-Torres, Bernardo A Martinez-Guerra, Carla M Román-Montes, Luis Ostrosky-Zeichner, Alfredo Ponce-de-Leon, María F Gonzalez-Lara
{"title":"Development of a new clinical prediction rule for candidaemia.","authors":"Diego U Vazquez-Omana, Paula Beltran-Reyes, Jorge E Alonso-Montoya, Nayeli E Avalos-Celis, Edgar D Centeno-Matus, Sandra Rajme-Lopez, Karla M Tamez-Torres, Bernardo A Martinez-Guerra, Carla M Román-Montes, Luis Ostrosky-Zeichner, Alfredo Ponce-de-Leon, María F Gonzalez-Lara","doi":"10.1186/s12879-025-12459-0","DOIUrl":"https://doi.org/10.1186/s12879-025-12459-0","url":null,"abstract":"","PeriodicalId":8981,"journal":{"name":"BMC Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145931999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Prevalence and determinants of intestinal parasitic infections among children under five years attending Bule Hora University Teaching Hospital, Southern Ethiopia.","authors":"Mohamed Esmael Gena, Siratu Seyoum, Abiy Ayele Angelo","doi":"10.1186/s12879-026-12515-3","DOIUrl":"https://doi.org/10.1186/s12879-026-12515-3","url":null,"abstract":"","PeriodicalId":8981,"journal":{"name":"BMC Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145916691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-08DOI: 10.1186/s12879-025-12360-w
Hong Chen, Qiu-Feng Han, Fan Wu
{"title":"Distribution of pathogenic bacteria and analysis of risk factors for surgical site infections after hepatobiliary surgery.","authors":"Hong Chen, Qiu-Feng Han, Fan Wu","doi":"10.1186/s12879-025-12360-w","DOIUrl":"https://doi.org/10.1186/s12879-025-12360-w","url":null,"abstract":"","PeriodicalId":8981,"journal":{"name":"BMC Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145916710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-07DOI: 10.1186/s12879-025-12332-0
Lan Wen, Wei Ning, Hui-Ping Zhang, Fang Peng, Miao-Miao Wang, Fei-Fan Huang, Bin Zhang, Jie-Peng Huang, Ming-Zhong Xu, Bin Tian
Background: Mycobacterium tuberculosis (MTB) retains infectivity for 8-10 days in dry dust and survives up to 6-8 months in humid environments. Dust accumulated in ventilation systems and air-conditioning filters serves as a critical reservoir for MTB aerosol transmission, making its detection vital for environmental risk assessment in settings prone to clustered outbreaks, such as schools and hospitals. However, molecular detection of MTB in dust is hindered by inhibitors (e.g., humic acids and heavy metals), leading to high false-negative rates, while conventional decontamination methods (e.g., acid-base treatments) compromise bacterial viability and efficiency.
Methods: This study developed a non-destructive Percoll density gradient centrifugation method applied to both H37Rv-spiked artificial dust matrices and real-world air-conditioning dust samples from tuberculosis wards, enabling efficient isolation of viable MTB. This approach leverages buoyant density-based separation to minimize inhibitory effects and maintain bacterial viability.
Results: (1) For H37Rv-spiked artificial dust (100-108 CFU/mL), total recovery rates were 31.75-73.96% with up to 73.5% of recovered bacteria retaining culturability; (2) The method showed no significant discrimination between live and heat-inactivated H37Rv: recovery rates of pure viable and heat-inactivated groups were comparable, and observed recoveries of live/heat-inactivated mixtures mostly matched expected values; (3) For 25 real-world dust samples, qPCR-positive detection rate increased from 56% (direct extraction) to 80%, MTB DNA abundance elevated nearly 10-fold (1,377.73 vs. 143.91 copies/mL), with reduced Ct values, minimized inhibitor interference, and improved amplification efficiency/template quality, enhancing high-risk ward identification.
Conclusion: This method is operationally simple, non-toxic, and biocompatible, achieving high total recovery of MTB cells while maintaining a high proportion of viable among the recovered bacteria. It offers substantial potential for environmental monitoring and downstream sequencing analysis, aiding in environmental risk assessment and management of clustered tuberculosis outbreaks.
{"title":"Non-continuous Percoll density gradient: a method for purifying Mycobacterium tuberculosis from dust.","authors":"Lan Wen, Wei Ning, Hui-Ping Zhang, Fang Peng, Miao-Miao Wang, Fei-Fan Huang, Bin Zhang, Jie-Peng Huang, Ming-Zhong Xu, Bin Tian","doi":"10.1186/s12879-025-12332-0","DOIUrl":"https://doi.org/10.1186/s12879-025-12332-0","url":null,"abstract":"<p><strong>Background: </strong>Mycobacterium tuberculosis (MTB) retains infectivity for 8-10 days in dry dust and survives up to 6-8 months in humid environments. Dust accumulated in ventilation systems and air-conditioning filters serves as a critical reservoir for MTB aerosol transmission, making its detection vital for environmental risk assessment in settings prone to clustered outbreaks, such as schools and hospitals. However, molecular detection of MTB in dust is hindered by inhibitors (e.g., humic acids and heavy metals), leading to high false-negative rates, while conventional decontamination methods (e.g., acid-base treatments) compromise bacterial viability and efficiency.</p><p><strong>Methods: </strong>This study developed a non-destructive Percoll density gradient centrifugation method applied to both H37Rv-spiked artificial dust matrices and real-world air-conditioning dust samples from tuberculosis wards, enabling efficient isolation of viable MTB. This approach leverages buoyant density-based separation to minimize inhibitory effects and maintain bacterial viability.</p><p><strong>Results: </strong>(1) For H37Rv-spiked artificial dust (10<sup>0</sup>-10<sup>8</sup> CFU/mL), total recovery rates were 31.75-73.96% with up to 73.5% of recovered bacteria retaining culturability; (2) The method showed no significant discrimination between live and heat-inactivated H37Rv: recovery rates of pure viable and heat-inactivated groups were comparable, and observed recoveries of live/heat-inactivated mixtures mostly matched expected values; (3) For 25 real-world dust samples, qPCR-positive detection rate increased from 56% (direct extraction) to 80%, MTB DNA abundance elevated nearly 10-fold (1,377.73 vs. 143.91 copies/mL), with reduced Ct values, minimized inhibitor interference, and improved amplification efficiency/template quality, enhancing high-risk ward identification.</p><p><strong>Conclusion: </strong>This method is operationally simple, non-toxic, and biocompatible, achieving high total recovery of MTB cells while maintaining a high proportion of viable among the recovered bacteria. It offers substantial potential for environmental monitoring and downstream sequencing analysis, aiding in environmental risk assessment and management of clustered tuberculosis outbreaks.</p>","PeriodicalId":8981,"journal":{"name":"BMC Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145916733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-07DOI: 10.1186/s12879-025-12304-4
Adrian Steulet, Piotr Hippner, Noriah Maraba, Lauren Jennings, Israel Rabothata, Rachel Mukora, Nokhanyo Xaba, Lihle Mchunu, Kavindhran Velen, Catherine Orrell, Salome Charalambous, Katherine Fielding
{"title":"Baseline determinants of adherence for drug-sensitive TB treatment in a South African prospective cohort: a focus on HIV infection and anti-retroviral therapy, clinical care access, and TB stigma.","authors":"Adrian Steulet, Piotr Hippner, Noriah Maraba, Lauren Jennings, Israel Rabothata, Rachel Mukora, Nokhanyo Xaba, Lihle Mchunu, Kavindhran Velen, Catherine Orrell, Salome Charalambous, Katherine Fielding","doi":"10.1186/s12879-025-12304-4","DOIUrl":"https://doi.org/10.1186/s12879-025-12304-4","url":null,"abstract":"","PeriodicalId":8981,"journal":{"name":"BMC Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145916656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-07DOI: 10.1186/s12879-025-12512-y
Hyeon Jae Jo, Sook-In Jung, Eu Suk Kim, Kyoung-Ho Song, Kyung-Hwa Park, Nam Joong Kim
{"title":"Characteristics of infectious spondylitis patients with normal white blood cell count and C-reactive protein level and associated factors.","authors":"Hyeon Jae Jo, Sook-In Jung, Eu Suk Kim, Kyoung-Ho Song, Kyung-Hwa Park, Nam Joong Kim","doi":"10.1186/s12879-025-12512-y","DOIUrl":"https://doi.org/10.1186/s12879-025-12512-y","url":null,"abstract":"","PeriodicalId":8981,"journal":{"name":"BMC Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145916714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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