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Diversity and distribution patterns of antennal sensilla in Hydropsychidae (Insecta, Trichoptera) 水螅科触角感受器的多样性及其分布模式(昆虫纲,毛翅目)
Q3 Agricultural and Biological Sciences Pub Date : 2021-12-30 DOI: 10.21638/spbu03.2021.403
K. A. Abu Diiak, M. Valuyskiy, S. Melnitsky, V. Ivanov
Structure and distribution of antennal sensilla were studied in males of 19 species of the caddisfly family Hydropsychidae by using scanning electron microscopy (SEM). Eleven types of sensilla were found: long trichoid, chaetoid, thick chaetoid, curved trichoid, coronary, basiconic, styloconic and four types of pseudoplacoid sensilla (mushroom-like, auricillic, ribbed, and T-shaped). Thick chaetoid, ribbed pseudoplacoid, and T-shaped pseudoplacoid sensilla were found only in Macronematinae. The great diversity of pseudoplacoid sensilla originated from a mushroom-like type, which also has a variable structure. Basal flagellomeres in the majority of studied species are equipped with ventrally positioned sensory fields of curved trichoid sensilla. In contrast to Arctopsychinae and Hydropsychinae, the increased number of these sensilla in the fields was noted for Diplectroninae and Smicrideinae. Most Macronematinae show a reduction of sensory fields and a strongly decreased average number of curved trichoid sensilla on distal segments. The great differences found in the studied family probably indicate a rapid function-related evolution of the antennal sensory surface structures in the caddisfly family Hydropsychidae.
利用扫描电子显微镜(SEM)研究了水螅科19种昆虫雄性触角感受器的结构和分布。发现了11种类型的感受器:长毛状、毛状、厚毛状、弯曲毛状、冠状、基部、柄状和四种类型的假片状感受器(蘑菇状、耳状、肋状和T形)。厚毛状、肋状假板状和T形假板状感觉器仅见于巨线虫科。假板状感受器的巨大多样性起源于蘑菇状感受器,它也具有可变的结构。大多数研究物种的基底鞭毛都具有位于腹侧的弯曲毛状感觉器的感觉区。与Arctopsycinae和Hydropsycinae相比,Dielectroninae和Smicrideinae在野外的感受器数量有所增加。大多数大线虫目显示感觉野减少,远端节段弯曲毛状感觉器的平均数量显著减少。在所研究的科中发现的巨大差异可能表明,水螅科的触角感觉表面结构与功能相关的快速进化。
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引用次数: 4
Hemocyte proteome of the Lake Baikal endemic Eulimnogammarus verrucosus (Crustacea: Amphipoda) sheds light on immune-related proteins 贝加尔湖地方性疣状Eulimnogamarus(甲壳纲:两栖纲)的血细胞蛋白质组揭示了免疫相关蛋白
Q3 Agricultural and Biological Sciences Pub Date : 2021-12-30 DOI: 10.21638/spbu03.2021.402
E. Zolotovskaya, A. Nazarova, A. Saranchina, A. Mutin, P. Drozdova, Y. Lubyaga, M. Timofeyev
Hemocytes are cells circulating in the hemolymph and playing an important role in crustacean immunity. These cells not only function as phagocytes but also express immune compounds to the hemolymph. Here we obtained hemocyte proteome of the endemic amphipod (Amphipoda, Crustacea) Eulimnogammarus verrucosus from Lake Baikal, the first hemocyte proteome of an amphipod, using liquid chromatography/tandem mass spectrometry (LC-MS/MS). A total of 1152 unique proteins were discovered with LC-MS/MS. We discovered both proteins directly involved in the immune response, such as pattern recognition proteins (C-type lectins), and compounds with antimicrobial activity (ctenidin and anti-lipopolysaccharide factor/scygonadin). Moreover, hemocyanins which may act as a phenoloxidase and C-type lectins were among the most diverse protein groups in the hemocyte proteome. The obtained data can be useful for further studies of immune components and mechanisms in Baikal amphipods.
血细胞是在血淋巴中循环的细胞,在甲壳类动物的免疫中起着重要作用。这些细胞不仅具有吞噬细胞的功能,而且还向血淋巴表达免疫化合物。在这里,我们使用液相色谱/串联质谱法(LC-MS/MS)获得了来自贝加尔湖的地方性两栖动物(两栖纲,甲壳纲)疣状Eulimnogamarus的血细胞蛋白质组,这是第一个两栖动物的血细胞蛋白组。LC-MS/MS共发现1152种独特的蛋白质。我们发现了两种直接参与免疫反应的蛋白质,如模式识别蛋白(C型凝集素)和具有抗菌活性的化合物(ctenidin和抗脂多糖因子/奇果纳丁)。此外,可以作为酚氧化酶的血蓝蛋白和C型凝集素是血细胞蛋白质组中最多样化的蛋白质组之一。所获得的数据可用于贝加尔湖片脚类动物免疫成分和机制的进一步研究。
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引用次数: 1
Agaricoid and boletoid fungi of Russia: the modern country-scale checklist of scientific names based on literature data 俄罗斯的类真菌和类糖真菌:基于文献资料的现代国家级学名清单
Q3 Agricultural and Biological Sciences Pub Date : 2021-12-30 DOI: 10.21638/spbu03.2021.404
S. Bolshakov, L. Kalinina, E. Palomozhnykh, K. Potapov, D. Ageyev, S. Arslanov, N. Filippova, M. Palamarchuk, D. Tomchin, E. Voronina
The authors of this paper summarize the majority of published data on the distribution of agaricoid and boletoid fungi recorded in Russia, covering the period from 1824 through 2020. A comprehensive list of 6867 scientific names based on 954 publications was compiled for the first time for the whole territory of Russia. All records have been checked through Index Fungorum. The work consists of a review section and five appendices. The review section discusses the intensity of field research and accumulation of data on the distribution of agaricoid and boletoid fungi in Russia, both historically and in its current state. The authors discuss the current state of knowledge on the biodiversity of regions of Russia and point out blank spots, thus providing a reference and an “action plan” for the future. Appendix A presents a list of 6142 taxa unambiguously ascribed to 3246 accepted current names. Appendix B contains 727 names that cannot be ascribed to any accepted current names unequivocally, with reasons given (e.g., no current name, wrong authors’ citations, absence from Index Fungorum). Names from both checklists are supplemented with data on the distribution of these taxa within the Russian Federation and references to published records. Appendix C contains a list of accepted current names reported from only one region. Appendix D is an overview of the nearly 200 years of research of agaricoid and boletoid fungi for all regions of Russia. Appendix E is a list of references used for checklists and study history preparation.
本文作者总结了1824年至2020年期间在俄罗斯记录的大多数已发表的真菌和蘑菇状真菌分布数据。根据954份出版物,首次为俄罗斯全境编制了6867个科学名称的综合清单。所有记录都已通过Index Fungorum进行了检查。这项工作包括一个审查部分和五个附录。综述部分讨论了俄罗斯历史上和目前真菌分布的实地研究强度和数据积累。作者讨论了俄罗斯各地区生物多样性的知识现状,并指出了空白点,从而为未来提供了参考和“行动计划”。附录A列出了6142个分类群,明确归属于3246个公认的当前名称。附录B包含727个无法明确归属于任何公认的当前名称的名称,并给出了原因(例如,没有当前名称、引用错误的作者、没有索引真菌)。这两份清单中的名称都补充了这些分类群在俄罗斯联邦境内的分布数据和已公布记录的参考资料。附录C仅包含一个地区报告的可接受的当前名称列表。附录D概述了俄罗斯所有地区近200年来对蘑菇状和蘑菇状真菌的研究。附录E是用于检查表和研究史准备的参考文献列表。
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引用次数: 6
Nitrogen use by plants and nitrogen flows after application of standard and biomodified nitrogen fertilizers on barley 标准氮肥和生物改性氮肥在大麦上施用后植物对氮的利用及氮的流动
Q3 Agricultural and Biological Sciences Pub Date : 2021-12-30 DOI: 10.21638/spbu03.2021.401
A. Zavalin, V. Chebotar, A. Alferov, Lyudmila Chernova, E. Shcherbakova, E. Chizhevskaya
The aim of our study was to assess the efficiency of application of biomodified nitrogen fertilizers for barley, to reveal the sources of nitrogen used for biomass formation with the use of the 15N stable isotope, and to study nitrogen flows in the system of fertilizers–soil–plants–atmosphere. We demonstrated in a model experiment the ability of the plant growth-promoting bacteria Bacillus subtilis Ch-13 to move from the granules of mineral fertilizers to plant roots and to colonize them effectively. The effectiveness of biomodified nitrogen fertilizers for barley, Nur variety, was assessed in a microfield trial. After the application of biomodified nitrogen fertilizers, the accumulation of 15N in the plants increased by 2–5 %, its incorporation in the soil decreased and gaseous losses were decreased by 7 % as compared with the use of the usual forms of fertilizers. The application of biomodified nitrogen fertilizers can be used in agricultural practice as a novel technology to regulate nitrogen flows in the system of fertilizers–soil–plants–atmosphere.
本研究旨在评价大麦生物氮肥的施用效率,利用15N稳定同位素揭示用于生物质形成的氮的来源,并研究肥料-土壤-植物-大气系统中的氮流。我们在一个模型实验中证明了植物生长促进细菌枯草芽孢杆菌Ch-13从矿物肥料颗粒移动到植物根部并有效定植的能力。通过微田试验,评价了生物氮肥在Nur品种大麦上的施用效果。施用生物氮肥后,植株中15N的积累量比施用常规氮肥增加2 - 5%,土壤中15N的掺入量减少,气体损失量减少7%。生物改性氮肥作为调控肥料-土壤-植物-大气系统氮流的一种新技术,可用于农业实践。
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引用次数: 3
Metaproteomic Comparison of Cryoconite Communities from Caucasian and Novaya Zemlya Glaciers 高加索和新地岛冰川冰晶群落的元蛋白质组学比较
Q3 Agricultural and Biological Sciences Pub Date : 2021-11-30 DOI: 10.21203/rs.3.rs-999957/v1
B. Zainullina, I. Babkina, A. Lobov, R. Tembotov, E. Abakumov
Anthropogenic pollution strongly affects glacial microbiological communities and promotes glacial melting. In the early stages of glacial melting formation of small cylindrical holes (cryoconite) occurs. While the microbiome of cryoconite is well described, the effect of anthropogenic pollution on cryoconite microbiological communities still has not been fully understood. Thus, we performed an unbiased functional comparison of the cryoconite communities from the highly polluted Caucasian glaciers and from less polluted glaciers in Novaya Zemlya. For this purpose, we used the shotgun metaproteomics approach which has not been used for cryoconite microbiome analysis previously. We identified 475 protein groups, a third of which were found in both glaciers. Cryoconites in both glaciers have similar microbiological communities with Cyanobacteria as dominant phyla. Nevertheless, we found a slight shift from the dominance of phototrophic Cyanobacteria in Novaya Zemlya to heterotrophic bacteria in the Caucasus. We assume that it might be caused by anthropogenic pollution, but other factors such as differences in seasonal dynamics of microbiological communities should be tested in the future.
人为污染强烈影响冰川微生物群落,并促进冰川融化。在冰川融化的早期阶段,会形成小的圆柱形孔(冰锥岩)。尽管人们对冰晶石的微生物组有很好的描述,但人类污染对冰晶石微生物群落的影响仍未完全了解。因此,我们对Novaya-Zemlya污染严重的高加索冰川和污染较轻的冰川的冰锥岩群落进行了无偏的功能比较。为此,我们使用了霰弹枪超蛋白质组学方法,该方法以前从未用于冷冻锥菌微生物组分析。我们鉴定了475个蛋白质组,其中三分之一在两座冰川中都有发现。两座冰川中的冰锥菌具有相似的微生物群落,蓝细菌是优势门。尽管如此,我们发现Novaya Zemlya的光养蓝细菌的优势向高加索的异养细菌略有转变。我们假设它可能是由人为污染引起的,但其他因素,如微生物群落季节动态的差异,应该在未来进行测试。
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引用次数: 0
Transcriptomic analysis of sym28 and sym29 supernodulating mutants of pea (Pisum sativum L.) under complex inoculation with beneficial microorganisms 有益微生物复合接种对豌豆(Pisum sativum L.) sym28和sym29超结突变体转录组学分析
Q3 Agricultural and Biological Sciences Pub Date : 2021-11-12 DOI: 10.21638/spbu03.2021.301
V. Zhukov, E. Zorin, A. Zhernakov, A. Afonin, G. Akhtemova, Andrej Bovin, A. Dolgikh, A. Gorshkov, E. Gribchenko, K. Ivanova, A. Kirienko, A. Kitaeva, M. Kliukova, O. Kulaeva, P. Kusakin, I. Leppyanen, O. Pavlova, D. Romanyuk, E. Rudaya, T. Serova, O. Shtark, A. Sulima, A. Tsyganova, E. Vasileva, E. Dolgikh, V. Tsyganov, I. Tikhonovich
The garden pea (Pisum sativum L.), like most members of Fabaceae family, is capable of forming symbioses with beneficial soil microorganisms such as nodule bacteria (rhizobia), arbuscular mycorrhizal (AM) fungi and plant growth promoting bacteria (PGPB). The autoregulation of nodulation (AON) system is known to play an important role in controlling both the number of nodules and the level of root colonization by AM via root-to-shoot signaling mediated by CLAVATA/ESR-related (CLE) peptides and their receptors. In the pea, mutations in genes Sym28 (CLV2-like) and Sym29 (CLV1-like), which encode receptors for CLE peptides, lead to the supernodulation phenotype, i.e., excessive nodule formation. The aim of the present study was to analyze the response of pea cv. ‘Frisson’ (wild type) and mutants P64 (sym28) and P88 (sym29) to complex inoculation with rhizobia, AM fungi and PGPB, with regard to biomass accumulation, yield and transcriptomic alterations. The plants were grown in quartz sand for 2 and 4 weeks after inoculation with either rhizobia (Rh) or complex inoculation with Rh + AM, Rh + PGPB, and Rh+AM+PGPB, and the biomass and yield were assessed. Transcriptome sequencing of whole shoots and roots was performed using a modified RNAseq protocol named MACE (Massive Analysis of cDNA Ends). In the experimental conditions, P88 (sym29) plants demonstrated the best biomass accumulation and yield, as compared to the wild type and P64 (sym28) plants, whereas P64 (sym28) had the lowest rate of biomass and seed yield. The transcriptome analysis showed that both supernodulating mutants more actively responded to biotic and abiotic factors than the wild-type plants and demonstrated increased expression of genes characteristic to late stages of nodule development. The roots of P64 (sym28) plants responded to AM+Rh treatment with upregulation of genes encoding plastid proteins, which can be connected with the activation of carotenoid biosynthesis (namely, the non-mevalonate pathway that takes place in root plastids). The more active response to symbionts in P88 (sym29) plants, as compared to cv. ‘Frisson’, was associated with counterregulation of transcripts involved in chloroplast functioning and development in leaves, which accompanies successful plant development in symbiotic conditions. Finally, the effect of retardation of plant aging upon mycorrhization on a transcriptomic level was recorded for cv. ‘Frisson’ but not for P64 (sym28) and P88 (sym29) mutants, which points towards its possible connection with the AON system. The results of this work link the plant’s autoregulation with the responsiveness to inoculation with beneficial soil microorganisms.
豌豆(Pisum sativum L.)与大多数豆科植物一样,能够与根瘤菌(rhizobia)、丛枝菌根(AM)真菌和植物生长促进菌(PGPB)等有益土壤微生物形成共生关系。结瘤自动调节(AON)系统在AM通过CLAVATA/ esr相关肽及其受体介导的根到茎信号传导控制根瘤数量和根定殖水平方面发挥重要作用。在豌豆中,编码CLE肽受体的基因Sym28 (clv2样)和Sym29 (clv1样)的突变导致了超结表型,即过度的结节形成。本研究的目的是分析豌豆cv的反应。对‘Frisson’(野生型)和突变体P64 (sym28)和P88 (sym29)与根瘤菌、AM真菌和PGPB复合接种对生物量积累、产量和转录组的影响。分别接种根瘤菌(Rh)或复合接种Rh+AM、Rh+ PGPB和Rh+AM+PGPB,在石英砂中培养2周和4周,评估生物量和产量。全茎和全根的转录组测序使用改良的RNAseq协议MACE (Massive Analysis of cDNA Ends)进行。在实验条件下,与野生型和P64 (sym28)植株相比,P88 (sym29)植株的生物量积累和产量最好,而P64 (sym28)植株的生物量和产量最低。转录组分析表明,这两种超结突变体对生物和非生物因素的响应都比野生型植物更积极,并且在结瘤发育后期表现出特征基因的表达增加。P64 (sym28)植株的根对AM+Rh处理的反应是编码质体蛋白的基因上调,这可能与类胡萝卜素生物合成的激活有关(即发生在根质体中的非甲羟酸途径)。P88 (sym29)植株对共生体的响应较cv。“Frisson”与叶片叶绿体功能和发育相关的转录物的反调节有关,这伴随着共生条件下植物的成功发育。最后,在转录组学水平上记录了延缓植株衰老对菌根形成的影响。但对P64 (sym28)和P88 (sym29)突变体不适用,这表明它可能与AON系统有关。这项工作的结果将植物的自调节与对接种有益土壤微生物的反应联系起来。
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引用次数: 1
Presence of Notahypsibius pallidoides (Tardigrada: Hypsibiidae) in the fauna of Russia confirmed with the methods of DNA barcoding 用DNA条形码方法证实了俄罗斯区系中存在恙螨(缓步目:恙螨科)
Q3 Agricultural and Biological Sciences Pub Date : 2021-11-12 DOI: 10.21638/spbu03.2021.309
Denis Tumanov
A brief report is given on the tardigrade species Notahypsibius pallidoides, discovered in a moss cushion in the vicinity of Saint Petersburg. Morphological identification of the species is supported with the analysis of obtained sequences of nuclear and mitochondrial DNA barcode genes (ITS-2 and COI). The current state of knowledge of Russian fauna of Tardigrada is characterised, and all available data on the distribution of Notahypsibius pallidoides and its genetic variability are summarised. The data obtained in this study by methods of DNA barcoding demonstrated that studied specimens of the tardigrada population isolated in Russia belong to the same species (Notahypsibius pallidoides) as the control specimens isolated in Austria though represent another haplotype thus confirming the presence of Notahypsibius pallidoides for the fauna of Russia.
一份关于在圣彼得堡附近的苔藓垫中发现的缓步动物Notahpsbius pallidoides的简要报告。通过对获得的细胞核和线粒体DNA条形码基因(ITS-2和COI)序列的分析,支持了该物种的形态学鉴定。对俄罗斯Tardigrada动物群的现有知识状况进行了描述,并总结了所有关于Notahpsbius pallidoides分布及其遗传变异的可用数据。本研究中通过DNA条形码方法获得的数据表明,在俄罗斯分离的缓步动物种群的研究标本与在奥地利分离的对照标本属于同一物种(苍白灰蝶),尽管代表了另一个单倍型,从而证实了俄罗斯动物群中存在苍白灰蝶。
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引用次数: 3
Analysis of the polydispersity of soil-like bodies in glacier environments by the laser light scattering (diffraction) method 用激光散射(衍射)法分析冰川环境中类土体的多分散性
Q3 Agricultural and Biological Sciences Pub Date : 2021-11-12 DOI: 10.21638/spbu03.2021.302
E. Abakumov, T. Nizamutdinov, V. Polyakov
This study presents the results of polydispersity analysis of soil-like bodies from two various polar regions using the laser light scattering method. The differences in the particle size distribution of cryoconite samples from the Anuchin Glacier (Antarctica) and the Mushketov Glacier (Arctic) are described. The samples obtained from the Mushketov Glacier are characterized by a finer particle size distribution than samples collected on the Anuchin Glacier. While comparing our results with previously published studies, it was found that the method of laser light scattering shows a lower content of small fractions (<0.05 mm) compared to the classical methods of sedimentation, since these methods are based on fundamentally different physical principles. The laser method used requires low amounts of samples (0.2–0.5 g), while the classical sedimentary method uses a higher gravimetric portion of cryoconite (5–10 g), which is critical for field sampling.
本文介绍了用激光散射方法对两个不同极地的类土体进行多分散性分析的结果。介绍了南极阿努钦冰川和北极穆什凯托夫冰川冰晶样品粒度分布的差异。从穆什凯托夫冰川获得的样本比在阿努钦冰川收集的样本具有更细的粒度分布。在将我们的研究结果与先前发表的研究结果进行比较时,发现激光散射方法与经典的沉积方法相比,显示出更低的小组分(<0.05 mm)的含量,因为这些方法基于根本不同的物理原理。激光法所需的样品量很少(0.2-0.5 g),而经典的沉积法使用的低温晶砂重量较高(5-10 g),这对现场采样至关重要。
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引用次数: 3
Sexual dimorphism patterns of the White Sea threespine stickleback (Gasterosteus aculeatus) 白海三刺棘鱼(Gasterosteus aculeatus)的两性二态性模式
Q3 Agricultural and Biological Sciences Pub Date : 2021-11-12 DOI: 10.21638/spbu03.2021.307
A. Dorgham, U. Candolin, T. Ivanova, M. Ivanov, E. Nadtochii, A. Yurtseva, D. Lajus
Sexual dimorphism (SD) in the threespine stickleback Gasterosteus aculeatus reflects the different roles of the sexes in reproduction and their adaptations to different ecological niches. We quantified SD in one population of marine stickleback from the White Sea, collected during the spawning period from three spawning grounds, each at a distance of 5 km or less from the others. We used a landmark-based approach to quantify variation in 44 morphometric linear traits. In total, 749 females and 693 males were analyzed. In males, anterior body parts are larger — the base of the caudal fin and armor structures such as the first and second dorsal spines and the pelvic spine. Females have larger posterior bodies — the abdomen, pelvic girdle and the third dorsal spine. The SD of caudal body parts exhibits complex patterns. In White Sea threespine stickleback, SD patterns are generally similar to other populations of the species, but more often show male-biased patterns. Female-biased size SD may be associated with the female biased sex ratio of White Sea stickleback.
刺鱼的两性异形(SD)反映了两性在繁殖中的不同作用及其对不同生态位的适应。我们在白海的一个海洋刺鱼种群中量化了SD,该种群在产卵期从三个产卵场收集,每个产卵场与其他产卵场的距离不超过5公里。我们使用了一种基于里程碑的方法来量化44个形态计量线性性状的变异。总共分析了749名女性和693名男性。雄性的身体前部更大——尾鳍的底部和第一、第二背脊和骨盆等盔甲结构。雌性有更大的后部身体——腹部、骨盆带和第三背脊。尾部SD表现出复杂的形态。在白海三刺鱼中,SD模式通常与该物种的其他种群相似,但更常见的是偏向雄性的模式。雌性偏大SD可能与白刺鱼雌性偏大的性别比有关。
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引用次数: 0
Wild-type p53-induced phosphatase sensitizes acute myeloid leukemia cells to conventional chemotherapy 野生型p53诱导的磷酸酶使急性髓系白血病细胞对常规化疗敏感
Q3 Agricultural and Biological Sciences Pub Date : 2021-11-12 DOI: 10.21638/spbu03.2021.308
V. Golotin, E. Belotserkovskaya, L. Girshova, A. Petukhov, A. Zaritsky, O. Demidov
Recently wild-type p53-induced phosphatase was implicated in the pathogenesis of acute myeloid leukemia (AML) and “pre-leukemia” myeloproliferative conditions. Here we decided to check how the strategy directed to phosphatase inhibition affected sensitivity to conventional chemotherapy. All experiments were conducted on AML cell lines cultivated in vitro. The levels of wild-type p53-induced phosphatase vary in different AML cell lines. The chemical compound GSK2830371 reduced levels of phosphatase and diminished its activity. GSK2830371 did not significantly change the cell cycle distribution of AML cells when used alone or in combination with the anti-cancer chemotherapeutic drug Cytosar but increased caspase-dependent PARP1 cleavage. In contrast with previous studies, we did not observe the negative effect of phosphatase activity inhibition and depletion on cells when a chemical inhibitor was used as monotherapy. Using a combination of GSK2830371 with Cytosar we were able to reduce the threshold of chemotherapy-dependent cytotoxicity and more efficiently eliminate leukemic cells. We propose considering inhibition of wild-type p53-induced phosphatase as a prospective strategy in improving anti-AML therapy.
最近,野生型p53诱导的磷酸酶与急性髓细胞白血病(AML)和“白血病前期”骨髓增殖条件的发病机制有关。在这里,我们决定检查针对磷酸酶抑制的策略如何影响对传统化疗的敏感性。所有实验均在体外培养的AML细胞系上进行。野生型p53诱导的磷酸酶水平在不同的AML细胞系中不同。化合物GSK2830371降低了磷酸酶水平并降低了其活性。当单独使用或与抗癌化疗药物Cytosar联合使用时,GSK2830371没有显著改变AML细胞的细胞周期分布,但增加了胱天蛋白酶依赖性PARP1的切割。与之前的研究相比,当化学抑制剂用作单一疗法时,我们没有观察到磷酸酶活性抑制和耗竭对细胞的负面影响。使用GSK2830371和Cytosar的组合,我们能够降低化疗依赖性细胞毒性的阈值,并更有效地消除白血病细胞。我们建议将抑制野生型p53诱导的磷酸酶作为改进抗AML治疗的前瞻性策略。
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引用次数: 0
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Biological Communications
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