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The crocodilian forearm and wrist: biomechanics and functional morphology 鳄鱼前臂和手腕:生物力学和功能形态学
Q3 Agricultural and Biological Sciences Pub Date : 2022-10-10 DOI: 10.21638/spbu03.2022.304
D. Pashchenko
An attempt has been made to explain the features of the wrist structure of crocodiles, which sharply distinguish them from other reptiles. Biomechanical model of a crocodilian forearm and manus is created with using of the vector contours method from the theory of mechanisms and machines. The key role of the V finger in the manus stability during the stance phase is shown. On the basis of this data, it is concluded that there is no bipedal stage in evolutionary history of crocodiles and their high specialization for quadrupedal parasagittal running with the emergence of a gallop as a result. The special way of parasagittal forelimb posture of the crocodiles offered to name instant parasagittality.
人们试图解释鳄鱼手腕结构的特征,这是鳄鱼与其他爬行动物的明显区别。利用机械力学理论中的矢量轮廓法,建立了鳄鱼前臂和手的生物力学模型。在站立阶段,V指在手稳定性中的关键作用被展示出来。根据这些数据,我们可以得出结论,在鳄鱼的进化史上不存在双足阶段,它们的高度专门化是四足的副单趾奔跑,从而出现了飞奔。鳄鱼特殊的副矢状前肢姿势为其命名提供了依据。
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引用次数: 0
Genetic diversity of rhizobial strains isolated from the relict legumes Gueldenstaedtia monophylla and G. verna growing in the republics of Altai and Buryatia (Russia) 阿尔泰共和国和布里亚特共和国(俄罗斯)残留豆科植物Gueldenstaedtia monophylla和G.verna中分离的根瘤菌菌株的遗传多样性
Q3 Agricultural and Biological Sciences Pub Date : 2022-10-10 DOI: 10.21638/spbu03.2022.301
D. Karlov, A. Sazanova, P. Guro, I. Kuznetsova, A. Verkhozina, A. Belimov, V. Safronova
For the first time, bacteria were isolated and identified from the root nodules of relict legumes Gueldenstaedtia monophylla Fisch. and G. verna (Georgi) Boriss. growing in the republics of Altai and Buryatia. The taxonomic position of the 29 obtained isolates was determined by sequencing the 16S rRNA gene (rrs). Showing a significant biodiversity, the isolates from G. monophylla and G. verna belonged to five genera of the order Rhizobiales: Mesorhizobium and Phyllobacterium (family Phyllobacteriaceae), Rhizobium (family Rhizobiaceae), Bosea (family Boseaceae), Bradyrhizobium (family Bradyrhizobiaceae). Three isolates which belonged to the species Bradyrhizobium valentinum and Rhizobium alamii showed 100 % of rrs-similarity with the type strains B. valentinum LmjM3T and R. alamii GBV016T, respectively. Six isolates of the genera Bosea and Rhizobium had a low level of rrs-similarity with the closest type strains (less than 99.5 %), which indicates that they may be assigned to new species. The data obtained can be used to itemise taxonomy within the order Rhizobiales, as well as to reveal the mechanisms of the formation of specific plant-microbial relationships during the evolution of symbiosis by studying the intermediate link between the extinct and modern rhizobia-legume symbiotic systems.
首次从残留的豆科植物Gueldenstaedtia monopylla Fisch的根瘤中分离鉴定出细菌。和G.verna(Georgi)Boriss。生长在阿尔泰共和国和布里亚特共和国。通过对16S rRNA基因(rrs)进行测序,确定了29个分离株的分类位置。从单叶G.monophylla和G.verna分离的菌株属于根瘤菌目五个属:中根瘤菌和叶菌属(叶菌科)、根瘤菌属(根瘤菌科),Bosea属(Boseaceae),慢生根瘤菌属。三株属于Bradyrhizobium valentinum和Rhizobium alamii的菌株分别与B.valentinum LmjM3T和R.alamii GBV016T型菌株表现出100%的rrs相似性。Bosea属和Rhizobium属的6个分离株与最接近型菌株的rrs相似性较低(低于99.5%),这表明它们可能属于新种。所获得的数据可用于逐项列出根瘤菌目的分类学,并通过研究已灭绝和现代根瘤菌-豆类共生系统之间的中间联系,揭示共生进化过程中特定植物-微生物关系的形成机制。
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引用次数: 0
Isolation, culturing and 3D bioprinting equine myoblasts 马成肌细胞的分离、培养和3D生物打印
Q3 Agricultural and Biological Sciences Pub Date : 2022-10-10 DOI: 10.21638/spbu03.2022.302
Alexandr Aimaletdinov, M. Abyzova, I. Kurilov, Alina Yuferova, Catrin Rutland, A. Rizvanov, E. Zakirova
Isolating and culturing myoblasts is essential for techniques such as tissue regeneration and in vitro meat production. This research describes a protocol to isolate primary myoblasts from skeletal muscle of an adult horse. The equine primary myoblasts expressed markers specific to myoblasts and had multipotent potential capabilities with differentiation into chondrocytes, adipocytes and osteoblasts in vitro. The horse myoblasts did not adhere to Cytodex 3 and grew poorly on CultiSpher-S microcarriers during in vitro cultivation. Our studies showed that the use of GelMa bioink and ionic cross-linking did not have negative effects on cell proliferation at the beginning of cultivation. However, cells showed reduced proliferative activity by day 40 following in vitro culturing. The population of primary equine myoblasts obtained from an adult individual, and propagated on microcarriers and bioink, did not meet the requirements of the regenerative veterinary and manufacturing meat in vitro regarding the quantity and quality of the cells required. Nonetheless, further optimization of the cell scaling up process, including both microcarriers and/or the bioreactor program and bioprinting, is still important.
分离和培养成肌细胞对于组织再生和体外肉类生产等技术至关重要。这项研究描述了一种从成年马骨骼肌中分离原代成肌细胞的方案。马原代成肌细胞表达成肌细胞特异性的标志物,并具有在体外分化为软骨细胞、脂肪细胞和成骨细胞的多能潜能。马成肌细胞不粘附于Cytodex 3,并且在体外培养过程中在CultiSpher-S微载体上生长不良。我们的研究表明,在培养开始时,使用GelMa生物墨水和离子交联对细胞增殖没有负面影响。然而,在体外培养后第40天,细胞显示出增殖活性降低。从成年个体获得并在微载体和生物墨水上繁殖的原代马成肌细胞群体在所需细胞的数量和质量方面不符合再生兽医和体外制造肉类的要求。尽管如此,进一步优化细胞放大过程,包括微载体和/或生物反应器程序和生物打印,仍然很重要。
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引用次数: 1
Preferable and limiting conditions of trans-Palearctic Orthocephalus species (Heteroptera: Miridae) 跨北极原头类物种的优选和限制条件(异翅目:Miridae)
Q3 Agricultural and Biological Sciences Pub Date : 2022-10-10 DOI: 10.21638/spbu03.2022.305
A. Namyatova
The environmental conditions limiting the distribution of trans-Palearctic species from various groups remain poorly studied. This work addresses the topic from three perspectives: (1) analysis of climatic variables, biomes and climate zones corresponding to the actual records; (2) assessment of environmental niches and contribution of biomes to those niches; (3) comparison of the distributional limits of trans-Palearctic and more local species. The genus Orthocephalus (Insecta: Heteroptera: Miridae) is used as a model taxon. The results have demonstrated that all trans-Palearctic species of this genus are known from numerous biomes and climate zones, but each of them has unique preferences. Biomes significantly contribute to the environmental niches of some of those species. None of the trans-Palearctic species were recorded from the hot steppe and desert climate zones, which occupy large areas in the Palearctic, although certain rare and local species inhabit those regions. This means that the trans-Palearctic species cannot easily adapt to those conditions.
限制来自不同群体的跨北极物种分布的环境条件仍然研究不足。这项工作从三个角度解决了这个问题:(1)分析与实际记录相对应的气候变量、生物群落和气候带;(2) 评估环境生态位和生物群落对这些生态位的贡献;(3) 跨北极和更多本地物种分布界限的比较。正头虫属(昆虫纲:异翅目:Miridae)被用作模式分类单元。研究结果表明,该属的所有跨北极物种都来自许多生物群落和气候区,但每一种都有独特的偏好。生物特征对其中一些物种的环境生态位做出了重大贡献。没有一种跨北极物种是在炎热的草原和沙漠气候区记录的,这些气候区占据了北极的大片地区,尽管这些地区居住着某些罕见的本地物种。这意味着跨北极物种无法轻易适应这些条件。
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引用次数: 0
Agar-based polyethylene glycol (PEG) infusion model for pea (Pisum sativum L.) — perspectives of translation to legume crop plants 豌豆(Pisum sativum L.)琼脂基聚乙二醇(PEG)灌注模型——转化为豆类作物的前景
Q3 Agricultural and Biological Sciences Pub Date : 2022-10-10 DOI: 10.21638/spbu03.2022.309
T. Leonova, Julia Shumilina, A. Kim, N. Frolova, L. Wessjohann, T. Bilova, A. Frolov
Due to the oncoming climate changes water deficit represents one of the most important abiotic stressors which dramatically affects crop productivity worldwide. Because of their importance as the principal source of food protein, legumes attract a special interest of plant scientists. Moreover, legumes are involved in symbiotic association with rhizobial bacteria, which is morphologically localized to root nodules. These structures are critical for fixation of atmospheric nitrogen and highly sensitive to drought. Therefore, new drought-tolerant legume cultivars need to be developed to meet the growing food demand. However, this requires a comprehensive knowledge of the molecular mechanisms behind the plant stress response. To access these mechanisms, adequate and reliable drought stress models need to be established. The agar-based polyethylene glycol (PEG) infusion model allows a physiologically relevant reduction of soil water potential (Ψw), although it is restricted to seedlings and does not give access to proteomics and metabolomics studies. Earlier, we successfully overcame this limitation and optimized this model for mature Arabidopsis plants. Here we make the next step forward and address its application to one of the major crop legumes — pea. Using a broad panel of physiological and biochemical markers, we comprehensively prove the applicability of this setup to legumes. The patterns of drought-related physiological changes are well-interpretable and generally resemble the stress response of plants grown in soil-based stop-watering models. Thus, the proposed model can be efficiently used in the study of stress-related metabolic adjustment in green parts, roots and root nodules of juvenile and flowering plants.
由于即将到来的气候变化,缺水是最重要的非生物压力源之一,极大地影响了全球作物生产力。由于豆类是食物蛋白质的主要来源,因此引起了植物科学家的特别兴趣。此外,豆类与根瘤菌共生,根瘤菌在形态上局限于根瘤。这些结构对大气氮的固定至关重要,并且对干旱高度敏感。因此,需要开发新的耐旱豆类品种来满足日益增长的粮食需求。然而,这需要对植物胁迫反应背后的分子机制有全面的了解。为了利用这些机制,需要建立充分和可靠的干旱胁迫模型。基于琼脂的聚乙二醇(PEG)输注模型允许生理相关的土壤水势(Ψw)降低,尽管它仅限于幼苗,并且不能进行蛋白质组学和代谢组学研究。早些时候,我们成功地克服了这一限制,并为成熟的拟南芥植物优化了该模型。在这里,我们向前迈出了下一步,并讨论了它在主要作物豆类豌豆中的应用。使用广泛的生理和生化标记,我们全面证明了这种设置对豆类的适用性。干旱相关的生理变化模式是可以很好地解释的,通常类似于在基于土壤的停止浇水模型中生长的植物的应激反应。因此,所提出的模型可以有效地用于幼树和开花植物绿色部位、根和根瘤的应激相关代谢调节研究。
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引用次数: 1
Auxin sensitivity improves production of rosmarinic acid in transformed hairy roots of Lavandula angustifolia 生长素敏感性提高狭叶薰衣草毛状根迷迭香酸的产生
Q3 Agricultural and Biological Sciences Pub Date : 2022-10-10 DOI: 10.21638/spbu03.2022.303
Azade Khadem, A. Bagheri, N. Moshtaghi, Fatemeh Keykha Akhar, A. Sharifi
Hairy root culture is a promising approach to improve production of plant secondary metabolites. The genes, which are located in T-DNA of a root-inducing plasmid, regulate auxin sensitivity of hairy roots. Therefore, this study was aimed to improve the growth and rosmarinic acid production of Lavandula angustifolia hairy roots. Lateral branches of hairy roots were transferred to ½ MS and ½ B5 liquid media. To assess auxin sensitivity, indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) with four different concentrations (0, 0.1, 0.5 and 1 mg/l) were also applied. The growth of hairy roots in ½ MS medium was two-fold higher than in ½ B5 medium. In addition, both auxins were found to significantly improve the growth of hairy roots whereas non-transformed roots stopped growing in the presence of the auxins. The highest dry weight and rosmarinic acid production of hairy roots were obtained from ½ MS medium supplemented with IBA irrespective of its concentration. As a result, the hairy roots grown in ½ MS medium supplemented with IBA produced the maximum amount of rosmarinic acid (7.98 mg/g dry weight of hairy roots). This first report of rosmarinic acid production in L. angustifolia hairy roots provides new insights into the auxin sensitivity of L. angustifolia hairy roots.
毛状根培养是提高植物次生代谢产物产量的一种很有前途的方法。这些基因位于根诱导质粒的T-DNA中,调节毛状根对生长素的敏感性。因此,本研究旨在改善狭叶薰衣草毛状根的生长和迷迭香酸的产生。将毛状根的侧枝转移到½MS和½B5液体培养基中。为了评估生长素的敏感性,还应用了四种不同浓度(0、0.1、0.5和1mg/l)的吲哚-3-乙酸(IAA)和吲哚-3-丁酸(IBA)。毛状根在½MS培养基中的生长比在½B5培养基中高出两倍。此外,发现两种生长素都能显著改善毛状根的生长,而未转化的根在生长素存在的情况下停止生长。毛状根的最高干重和迷迭香酸产量是在添加IBA的½MS培养基中获得的,无论其浓度如何。结果,在添加IBA的½MS培养基中生长的毛状根产生最大量的迷迭香酸(7.98mg/g毛状根干重)。首次报道了狭叶根中迷迭香酸的产生,为狭叶根对生长素的敏感性提供了新的见解。
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引用次数: 1
Changes in heart rate during electrical stimulation of the atrium in rainbow trout (Oncorhynchus mykiss) at low temperature 低温下虹鳟鱼心房电刺激时心率的变化
Q3 Agricultural and Biological Sciences Pub Date : 2022-06-24 DOI: 10.21638/spbu03.2022.204
N. Kibler, V. Nuzhny, D. Shmakov
In this work, we investigated the effect of high heart rate (HR) in vivo on the electrical properties and pumping functions of the heart ventricle of the rainbow trout (Oncorhynchus mykiss) at low ambient temperatures. HR was altered by atrial pacing. The electrocardiogram (ECG) parameters and hemodynamic parameters of the heart ventricle of rainbow trout adapted to a temperature of 5–7 °C were studied from the normal sinus rhythm (21.6 ± 4.9 bpm) to the maximum possible HR. Results show that a HR of about 60 bpm is the upper limit of the normal functional activity of the ventricle of the heart. An increase in heart rate up to 60 bpm leads to an increase in the PQ interval and QRS complex, a decrease in the QT interval on the ECG, and a violation of the rhythmic activity of the heart (i.e., the occurrence of extrasystole), as well as to a considerable change in the hemodynamic parameters of the ventricle of the heart and a decrease in its contractile properties. After a period of ventricular extrasystole for several minutes (10–15 min), the activity of the sinus node resumes but with a lower HR compared with the initial HR. The duration of the QRS complex recovers to the initial one, and the PQ and QT intervals increase. Maximum systolic pressure and end-diastolic pressure also return to their original values after extrasystolic contraction. After the experimental extrasystole, the phenomenon of the absence of an increase in HR is observed. The arising extrasystole probably has a functional nature and is one of the mechanisms of electromechanical homeostatic control in the heart.
在本研究中,我们研究了在低温环境下虹鳟鱼(Oncorhynchus mykiss)体内高心率(HR)对其心室电特性和泵送功能的影响。心房起搏改变心率。从正常窦性心律(21.6±4.9 bpm)到最大可能心率(HR),研究了虹鳟鱼在5 ~ 7℃环境下心脏心室的心电图(ECG)参数和血流动力学参数。结果表明,心率约为60bpm是心脏心室正常功能活动的上限。心率增加到60bpm会导致PQ间期和QRS复合体的增加,心电图上QT间期的减少,心脏节律性活动的破坏(即发生心动过速),以及心室血流动力学参数的显著变化及其收缩特性的降低。室性心动过速几分钟(10-15分钟)后,窦房结活动恢复,但心率比初始心率低。QRS复合体持续时间恢复到初始值,PQ和QT间期增加。最大收缩压和舒张末期压在收缩后也恢复到原来的值。实验性心动过速后,观察到心率不增高的现象。产生的心动过速可能具有功能性,是心脏机电稳态控制的机制之一。
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引用次数: 0
Rate of spontaneous polyploidization in haploid yeast Saccharomyces cerevisiae 单倍体酵母的自发多倍体率
Q3 Agricultural and Biological Sciences Pub Date : 2022-06-24 DOI: 10.21638/spbu03.2022.202
Yu. V. Andreychuk, A. Zhuk, E. Tarakhovskaya, S. Inge-Vechtomov, E. Stepchenkova
Polyploidization is involved in a variety of biological processes. It is one of the principal mechanisms of new species formation since it provides material for gene diversification and subsequent selection. Multiple cases of polyploidization were registered in different branches of the evolutionary tree of eukaryotes. Besides its role in evolution, polyploidization affects gene expression in living cells: pathological genome duplications often happen in cancer cells. The mechanisms and consequences of polyploidization are being studied extensively. However, quantitative determination of the polyploidization rate is challenging due to its low frequency and the absence of selective genetic markers that would phenotypically distinguish between haploids and polyploids. Our work describes a robust and straightforward method for discriminating haploid and polyploid states in the yeast Saccharomyces cerevisiae, a model organism for studying different aspects of polyploidization. The measurement of polyploidization rate showed that in yeast cells this process is mainly caused by autodiploidization rather than mating-type switching followed by hybridization.
多倍体化涉及多种生物过程。它是新物种形成的主要机制之一,因为它为基因多样化和随后的选择提供了材料。在真核生物进化树的不同分支中记录了多种多倍体现象。除了在进化中发挥作用外,多倍体化还影响活细胞中的基因表达:病理性基因组复制经常发生在癌细胞中。多倍体化的机制和后果正在被广泛研究。然而,多倍体率的定量测定是具有挑战性的,因为它的频率低,并且缺乏选择性的遗传标记,可以在表型上区分单倍体和多倍体。我们的工作描述了一种强大而直接的方法来区分酵母的单倍体和多倍体状态,酵母是研究多倍体不同方面的模式生物。多倍体化率的测定表明,酵母细胞多倍体化主要是由自身二倍体化引起的,而不是由杂交后的交配型转换引起的。
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引用次数: 1
Lateralization in the visual perception of a potential source of danger in free-ranging steppe marmots (Marmota bobak) 自由放养草原土拨鼠(Marmota bobak)潜在危险源视觉感知的横向化
Q3 Agricultural and Biological Sciences Pub Date : 2022-06-24 DOI: 10.21638/spbu03.2022.205
I. Demina, A. Dyomin, K. Karenina
A variety of vertebrate species display lateralization of visual perception of threat. However, the vast majority of studies were carried out in the laboratory conditions, and little is known about the relevance of this lateralization for animals in their natural environment. The aim of our work was to study lateralized visual perception of potential threat in the steppe marmot. The asymmetric use of visual fields when reacting to an approaching threat was assessed in two wild populations of marmots. The results showed that marmots predominantly used the right eye for threat monitoring. A significant impact of anthropogenic disturbance was found both within and between the populations. The right-sided preference was reduced in animals inhabiting areas with high degree of anthropogenic disturbance. The eye (left/right) a focal individual used for threat monitoring did not influence its flight initiation distance. Contrary to a previous study, our results do not suggest that the use of a particular eye affects the speed of threat assessment.
多种脊椎动物表现出对威胁的视觉感知偏侧化。然而,绝大多数研究都是在实验室条件下进行的,对于动物在自然环境中这种偏侧化的相关性知之甚少。本研究的目的是研究草原土拨鼠对潜在威胁的侧化视觉感知。在两个野生土拨鼠种群中,对接近的威胁作出反应时,对视野的不对称使用进行了评估。结果表明,土拨鼠主要使用右眼进行威胁监测。在种群内部和种群之间都发现了显著的人为干扰影响。在人为干扰程度高的地区,动物的右侧偏好降低。用于威胁监测的焦点个体的眼睛(左/右)不影响其飞行起始距离。与之前的研究相反,我们的研究结果并不表明使用特定的眼睛会影响威胁评估的速度。
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引用次数: 0
Lithobiotic cyanobacteria diversity of the Karelian Isthmus 卡累利阿地峡的石生蓝细菌多样性
Q3 Agricultural and Biological Sciences Pub Date : 2022-06-24 DOI: 10.21638/spbu03.2022.203
O. Rodina, D. Davydov, D. Vlasov
This work presents data obtained as a result of studying the composition of cyanobacteria in lithobiotic communities on various substrates (Ruskeala marble, rapakivi-granite, granite gneiss) in different light conditions on the territory of the Karelian Isthmus: Leningrad Oblast, Republic of Karelia, and South Finland. The species composition of cyanobacteria was revealed, and the species composition on certain types of substrates was analyzed. A total of 49 species of cyanobacteria were noted for the Republic of Karelia (13 of which were not previously recorded in this territory). The detailed taxonomic and environmental characteristics of species are given. Changes in the species diversity of cyanobacteria in connection with specific habitats are shown. The type of substrate, the degree of moisture, and illumination are noted as the main factors determining the diversity of cyanobacteria in lithobiotic communities.
这项工作提供了研究卡累利阿地峡地区列宁格勒州、卡累利亚共和国和南芬兰不同光照条件下各种基质(Ruskeala大理石、雷帕基维花岗岩、花岗片麻岩)上岩石生物群落中蓝藻组成的结果。揭示了蓝藻的物种组成,并分析了某些类型基质上的物种组成。卡累利阿共和国共发现49种蓝藻(其中13种以前没有在该领土记录)。给出了物种的详细分类和环境特征。显示了蓝藻物种多样性的变化与特定的栖息地有关。基质类型、湿度和光照是决定石生群落中蓝藻多样性的主要因素。
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引用次数: 0
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Biological Communications
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