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Immune protective, stress indicators, antioxidant, histopathological status, and heat shock protein gene expression impacts of dietary Bacillus spp. against heat shock in Nile tilapia, Oreochromis niloticus. 膳食芽孢杆菌对尼罗罗非鱼(Oreochromis niloticus)热休克的免疫保护、应激指标、抗氧化、组织病理学状态和热休克蛋白基因表达的影响。
IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES Pub Date : 2024-10-16 DOI: 10.1186/s12917-024-04303-5
Samia Elbahnaswy, Gehad E Elshopakey, Abdelwahab A Abdelwarith, Elsayed M Younis, Simon J Davies, Mai A M El-Son

This research evaluated the efficacy of mixed Bacillus strains probiotic supplements in mitigating acute thermal-induced stress in Nile tilapia (Oreochromis niloticus). Three experimental fish groups involved 135 Nile tilapia (49 ± 2 g); one control (no added probiotics), 0.5, and 1% of selected Bacillus strains (B. subtilis, B. licheniformis, and B. pumilus) for 58 days. After the feeding period, growth parameters, immunological parameters, stress biochemical markers, and antioxidant parameters in addition to genes related to stress and histopathological changes in fish, were assessed; subsequently subjected to heat shock at 36 ± 0.5 ◦C for 2 h. Before the heat challenge, our results exhibited a marked increase in the growth efficacy (P < 0.05), lower marked serum levels of triglycerides and cholesterol, and tissue malondialdehyde (MDA) levels along with significantly increased superoxide dismutase (SOD) and catalase (CAT) enzymes activity in fish-fed Bacillus probiotic at 0.5% concerning the control group (P < 0.05). There were no significant changes in the serum levels of glucose, cortisol, lactate, phagocytic activity, respiratory burst (ROS), total immunoglobulin Ig, alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), total protein, albumin, globulin, uric acid, urea, creatinine, as well as HSP70, GST, and GPx mRNA expression in most of the probiotic groups compared to the control group (P > 0.05). When Nile tilapia was exposed to heat stress, supplementation with Bacillus probiotic in the diet significantly decreased most of the indices related to serum biochemical (ALT (P < 0.01; P < 0.001), AST (P < 0.01), LDH (P < 0.01), urea (P < 0.05), and creatinine (P < 0.01)), triglycerides (P < 0.001; (P < 0.01)), cholesterol (P < 0.01; (P < 0.05)), glucose (P < 0.001), and cortisol (P < 0.01; (P < 0.05)), with tissue oxidative stress MDA levels (P < 0.05), and HSP70 mRNA expression (P < 0.01; P < 0.001), aligned with the stressed control group. In addition, a notable upsurge in the total protein, albumin, globulin, phagocytic and ROS activities, and total Ig, as well as the enzymatic antioxidant ability (SOD, CAT) (P < 0.01), with GST and GPx mRNA expression (P < 0.05; P < 0.01), were shown in fish-fed Bacillus spp. post-exposure compared with the stressed control group. Besides, no histopathological alterations were revealed in the spleen and brain of fish pre- and post-heat exposure. According to our findings, diet supplementation of Bacillus species has the potential to combat the suppressive effects of heat shock in Nile tilapia.

本研究评估了混合芽孢杆菌益生菌补充剂在缓解尼罗罗非鱼(Oreochromis niloticus)急性热诱导应激方面的功效。三个实验鱼组包括 135 尾尼罗罗非鱼(49 ± 2 克);一个对照组(不添加益生菌)、0.5% 和 1%的选定芽孢杆菌菌株(枯草芽孢杆菌、地衣芽孢杆菌和普米氏芽孢杆菌),共喂养 58 天。喂养期结束后,对鱼类的生长参数、免疫参数、应激生化指标、抗氧化参数以及与应激和组织病理学变化相关的基因进行了评估;随后在 36 ± 0.5 ◦C 的温度下进行热冲击 2 小时。当尼罗罗非鱼受到热应激时,在日粮中添加枯草芽孢杆菌益生菌可显著降低与血清生化有关的大多数指数(ALT(P<0.05))。
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引用次数: 0
Correction: Change in the plasma proteome associated with canine cognitive dysfunction syndrome (CCDS) in Thailand. 更正:泰国与犬认知功能障碍综合征 (CCDS) 相关的血浆蛋白质组变化。
IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES Pub Date : 2024-10-15 DOI: 10.1186/s12917-024-04330-2
Sataporn Phochantachinda, Boonrat Chantong, Onrapak Reamtong, Duangthip Chatchaisak
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引用次数: 0
Isolation and characterization of Streptococcus agalactiae inducing mass mortalities in cultured Nile tilapia (Oreochromis niloticus) with trials for disease control using zinc oxide nanoparticles and ethanolic leaf extracts of some medicinal plants. 诱导尼罗罗非鱼(Oreochromis niloticus)大量死亡的无乳链球菌的分离和特征描述,以及使用纳米氧化锌颗粒和一些药用植物的乙醇叶提取物控制疾病的试验。
IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES Pub Date : 2024-10-15 DOI: 10.1186/s12917-024-04298-z
Ebtsam Sayed Hassan Abdallah, Walaa Gomaa Mohamed Metwally, Soad Abdel Latief Hassan Bayoumi, Moataz Ahmed Mohamed Abdel Rahman, Mahmoud Mostafa Mahmoud

Background: Streptococcus agalactiae (Group B streptococcus, GBS) induces a serious infection that can harm not only aquatic life but also humans and other animals. In a fish farm in southern Egypt, Nile tilapia (Oreochromis niloticus) has developed an epidemic with clinical symptoms resembling piscine streptococcosis.

Results: Initial microscopic inspection of the affected fish brain and kidney indicated the presence of Gram-positive cocci. S. agalactiae was effectively isolated and identified using nucleotide homology of the 16S rRNA and species-specific PCR. The partial 16S rRNA sequence was deposited in the GenBank database at the NCBI and given the accession number MW599202. Genotyping using RAPD analysis indicated that the isolates in the present study belonged to the same genotypes and had the same origin. The challenge test, via immersion (9.2 × 107, 9.2 × 106, and 9.2 × 105 CFU/ml for 1 h) or intraperitoneal injection (4.6 × 107, 4.6 × 106, and 4.6 × 105 CFU/fish), elicited clinical symptoms resembling those of naturally infected fish with a mortality rate as high as 80%. The ability to create a biofilm as one of the pathogen virulence factors was verified. Zinc oxide nanoparticles and the ethanolic leaf extracts of nine medicinal plants demonstrated considerable antibacterial activities against the tested S. agalactiae strain with low minimum bactericidal concentrations (MBC) and minimum inhibitory concentrations (MIC). The ethanolic leaf extracts from Lantana camara and Aberia caffra showed potent antibacterial activity with MBC values of 0.24 and 0.485 mg/ml, and MIC values of 0.12 & 0.24 mg/ml, respectively.

Conclusion: This study isolated S. agalactiae from O. niloticus mortalities in a fish farm in Assiut, Egypt. The pathogen persists in fish environments and can escape through biofilm formation, suggesting it cannot be easily eliminated. However, promising findings were obtained with in vitro control employing zinc oxide nanoparticles and medicinal plant extracts. Nevertheless further in vivo research is needed.

背景:无乳链球菌(B 组链球菌,GBS)会引发严重感染,不仅会危害水生生物,还会危害人类和其他动物。在埃及南部的一个养鱼场,尼罗罗非鱼(Oreochromis niloticus)出现了一种流行病,临床症状类似于鱼类链球菌病:结果:对患病鱼的大脑和肾脏进行初步显微镜检查后发现存在革兰氏阳性球菌。利用 16S rRNA 的核苷酸同源性和物种特异性 PCR 技术,有效分离并鉴定了 S. agalactiae。部分 16S rRNA 序列已存入美国国家生物技术研究所(NCBI)的 GenBank 数据库,登录号为 MW599202。利用 RAPD 分析进行的基因分型表明,本研究中的分离物属于相同的基因型,具有相同的来源。通过浸泡(9.2×107、9.2×106 和 9.2×105 CFU/ml,浸泡 1 小时)或腹腔注射(4.6×107、4.6×106 和 4.6×105 CFU/条鱼)进行的挑战试验引起了与自然感染鱼相似的临床症状,死亡率高达 80%。病原体的毒力因素之一是形成生物膜的能力得到了验证。氧化锌纳米粒子和九种药用植物的乙醇叶提取物对测试的 S. agalactiae 菌株具有相当高的抗菌活性,最低杀菌浓度(MBC)和最低抑菌浓度(MIC)均较低。Lantana camara 和 Aberia caffra 的乙醇叶提取物显示出强大的抗菌活性,MBC 值分别为 0.24 和 0.485 毫克/毫升,MIC 值分别为 0.12 和 0.24 毫克/毫升:本研究从埃及阿苏特一家鱼类养殖场的黑线鳕死亡病例中分离出了 S. agalactiae。该病原体可在鱼类环境中存活,并可通过形成生物膜逃逸,这表明它不容易被消灭。不过,利用纳米氧化锌颗粒和药用植物提取物进行体外控制取得了令人鼓舞的结果。不过,还需要进一步的体内研究。
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引用次数: 0
Identification and genetic characterization of five novel bat coronaviruses from Yunnan, China. 中国云南五种新型蝙蝠冠状病毒的鉴定和遗传特征。
IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES Pub Date : 2024-10-12 DOI: 10.1186/s12917-024-04310-6
Qian Li, Yutong Hou, Baoyang Huang, Xiang Le, Binghui Wang, Xueshan Xia

Background: Coronaviruses (CoVs) represent a serious threat to human health and have become a major transmissible, endemic, and causative pathogen in humans; they represent a major health concern, given their ability to cause infectious diseases. Bats are natural hosts for diverse viruses. Many transmission events of CoVs and identification of multiple novel CoVs in bats has increased attention towards their capacity to serve as hosts for zoonotic viruses.

Results: In this study, 61 bats from Yunnan Province were analyzed, identifying seven CoVs, including three α- and two β-CoVs with full-genome sequences. Among the five identified alpha-CoVs, four belong to the Decacovirus subgenus and one to the Minunacovirus subgenus. Two beta-CoVs were also identified, both belonging to the Sarbecovirus subgenus.The genetic structures revealed similarities to known strains such as HKU10 and SARS-CoV-2, along with novel findings such as the Minunacovirus subgenus CoV YJ3c/f and unique ORF patterns. Our results demonstrated that strain JCC9 has a unique recombination pattern and shows a higher binding affinity to civet and pangolin ACE2 receptors, then the HpJC8xc strain transmits and recombines between hosts (bats), indicating a potential risk of crossing the interspecies barrier and infecting other animals.

Conclusions: The CoVs detected in the bats studied in this research exhibit high diversity. Genomic analysis revealed that CoVs in bats undergo frequent recombination events. Furthermore, recombination patterns and evolutionary analyses suggest that alpha-CoVs are more prone to cross-species transmission across different bat families/genera, whereas beta-CoVs demonstrate host specificity and tend to co-evolve with their bat hosts.Our finding suggest that bats, as hosts of CoVs, be constantly monitored to prevent outbreaks of new infections caused by viruses passing across interspecies barriers, and consequently, viral diseases in humans or livestock.

背景:冠状病毒(CoVs)对人类健康构成严重威胁,已成为人类主要的传播性、地方性和致病性病原体;由于它们能够引起传染性疾病,因此是一个重大的健康问题。蝙蝠是多种病毒的天然宿主。CoVs的多次传播和多种新型CoVs在蝙蝠中的发现使人们更加关注蝙蝠作为人畜共患病病毒宿主的能力:本研究对云南省的 61 只蝙蝠进行了分析,共鉴定出 7 种 CoV,包括 3 种具有全基因组序列的 α-CoV 和 2 种 β-CoV。在已鉴定的五种α-CoVs中,四种属于十日咳病毒亚属,一种属于米纳科病毒亚属。基因结构显示,JCC9与HKU10和SARS-CoV-2等已知毒株相似,但也有新发现,如Minunacovirus亚属CoV YJ3c/f和独特的ORF模式。我们的研究结果表明,JCC9株具有独特的重组模式,与果子狸和穿山甲ACE2受体的结合亲和力较高,而HpJC8xc株会在宿主(蝙蝠)之间传播和重组,这表明它有跨越种间屏障感染其他动物的潜在风险:本研究在蝙蝠体内检测到的 CoVs 表现出高度的多样性。基因组分析表明,蝙蝠体内的 CoV 频繁发生重组事件。此外,重组模式和进化分析表明,α-CoVs 更容易在不同蝙蝠科/属之间进行跨物种传播,而 beta-CoVs 则表现出宿主特异性,并倾向于与蝙蝠宿主共同进化。我们的研究结果表明,蝙蝠作为 CoVs 的宿主,应受到持续监控,以防止病毒跨越物种间屏障引起新的感染爆发,进而导致人类或家畜感染病毒性疾病。
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引用次数: 0
A field comparison study of two vaccine protocols against Erysipelothrix rhusiopathiae in two types of swine breeds in Spain. 在西班牙两种猪种中对两种预防红腹泻温热病疫苗方案进行实地比较研究。
IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES Pub Date : 2024-10-11 DOI: 10.1186/s12917-024-04065-0
E Sanchez-Tarifa, C Alonso, I Perez, L A García, A Fernández-Fontelo, O Gómez-Duran, B García-Morante, Francisco A García-Vázquez, I Hernández-Caravaca

Erysipelas still causes large economic losses to pig industry. Maternal immunity is critical to prevent erysipelas in young animals, thus, intensive vaccination protocols or practices focused on the improvement of the maternally derived immunity could provide substantial benefits. The present study evaluates potential changes in antibodies levels in sows and their offspring using two types of tests (commercial ELISA, Ingenasa or rSpaA415 ELISA) when two different vaccination programs (before farrowing or after farrowing) against Erysipelothrix rhusiopathiae were applied to sows from Iberian (A) or conventional Large White-Landrace (B) pig farms. The results showed a statistical correlation between titers found in sows and their one-week old piglets in both tests. The overall mean of (log) antibody titers in farm B measured by the commercial ELISA test was significantly higher in pre-farrowing vaccinated sows compared to the post-farrowing vaccine protocol (p = 0.0278). Additionally, using the rSpaA415 ELISA test, the overall mean of (log) antibody titers was significantly higher in pre-farrowing sows (p = 0.0056) compared to sows following post-farrowing vaccine protocol (p = 0.0003) or non- vaccinated sows. None of the above-mentioned differences were found in farm A. The overall mean of (log) antibody titers in piglets from the pre-farrowing vaccination protocol was significantly higher than piglets from the post-farrowing vaccination protocol in farm A (p = 0.0059; rSpaA415 ELISA) and farm B (p = 0.0168 and p = 0.0098 for the commercial and rSpaA415 ELISA data, respectively). Additionally, higher proportion of piglets from pre-farrowing vaccinated sows remained seropositive during the post-weaning period (days 42 to 84) compared to piglets from non-vaccinated or post-farrowing vaccinated groups in both farms A and B.

红臀疽仍然给养猪业造成巨大的经济损失。母源免疫对预防幼畜红痢至关重要,因此,强化疫苗接种方案或以提高母源免疫力为重点的做法可带来巨大收益。本研究使用两种检测方法(商业 ELISA、Ingenasa 或 rSpaA415 ELISA)评估了伊比利亚猪场(A)或传统大白兰氏猪场(B)的母猪在接种两种不同的红细胞体病疫苗方案(产仔前或产仔后)时,母猪及其后代体内抗体水平的潜在变化。结果显示,在这两项试验中,母猪及其一周龄仔猪的抗体滴度之间存在统计学相关性。在 B 猪场,通过商业 ELISA 试验测定的抗体滴度(对数)的总体平均值在排卵前接种疫苗的母猪中明显高于排卵后接种疫苗的母猪(p = 0.0278)。此外,使用 rSpaA415 酶联免疫吸附试验(ELISA),与接种后疫苗的母猪(p = 0.0003)或未接种疫苗的母猪相比,配种前母猪的抗体滴度(对数)总平均值明显更高(p = 0.0056)。在 A 猪场(p = 0.0059;rSpaA415 ELISA)和 B 猪场(商业数据和 rSpaA415 ELISA 数据分别为 p = 0.0168 和 p = 0.0098),排卵前接种方案的仔猪抗体滴度(对数)总平均值显著高于排卵后接种方案的仔猪。此外,在 A 猪场和 B 猪场,与未接种疫苗或接种疫苗后的仔猪相比,断奶前接种疫苗的母猪所产仔猪在断奶后(第 42 天至第 84 天)血清仍呈阳性的比例更高。
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引用次数: 0
Correction: Nano-selenium supplementation: improving growth, digestibility and mineral absorption in freshwater fish, Catla catla. 更正:纳米硒补充剂:改善淡水鱼 Catla catla 的生长、消化和矿物质吸收。
IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES Pub Date : 2024-10-11 DOI: 10.1186/s12917-024-04318-y
Nisar Ahmad, Syed Makhdoom Hussain, Shafaqat Ali, Muhammad Farrukh Tahir, Pallab K Sarker, Mudassar Shahid
{"title":"Correction: Nano-selenium supplementation: improving growth, digestibility and mineral absorption in freshwater fish, Catla catla.","authors":"Nisar Ahmad, Syed Makhdoom Hussain, Shafaqat Ali, Muhammad Farrukh Tahir, Pallab K Sarker, Mudassar Shahid","doi":"10.1186/s12917-024-04318-y","DOIUrl":"10.1186/s12917-024-04318-y","url":null,"abstract":"","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11468431/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142406030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nucleotide sequence variants, gene expression and serum profile of immune and antioxidant markers associated with bacterial diarrhea susceptibility in Barki lambs. 与巴基羔羊细菌性腹泻易感性相关的核苷酸序列变异、基因表达及血清免疫和抗氧化标记物概况
IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES Pub Date : 2024-10-11 DOI: 10.1186/s12917-024-04288-1
Asmaa Darwish, Eman Ebissy, Amani Hafez, Ahmed Ateya, Ahmed El-Sayed
<p><strong>Background: </strong>Despite the fact that diarrhea is more accurately described as a clinical symptom than a disease. Diarrhea is one of the most important issues in ovine medicine, particularly in lambs, and because of high morbidity and mortality rate, sluggish growth performance, and veterinary costs, it is believed to be a major source of economic loss. Salmonella and enterotoxigenic Escherichia coli are the most common and commercially significant agents responsible for diarrhea.</p><p><strong>Objective: </strong>The objective of this study was to monitor the nucleotide sequence variations, gene expression, serum inflammatory and oxidative stress biomarkers in diarrheic lambs. Another aim was to identify different pathotypes and virulence genes of Salmonella and E. coli causing diarrhea.</p><p><strong>Methodology: </strong>Blood samples were taken from 50 Barki who were diarrheal and 50 who appeared to be healthy, and then divided in 3 portions, with EDTA added to the first part for CBC, DNA and RNA extraction. The second sample received 5000 I.U. of heparin calcium, and a clean plain tube was used for the third component. The second and third sections were centrifuged to extract serum and plasma until the biochemical and immunological analysis was completed. Fecal samples were collected for bacteriological examination, and the bacteria were identified by PCR analysis. PCR-DNA sequencing was conducted for immune (SELL, JAK2, SLC11A1, IL10, FEZF1, NCF4, LITAF, SBD2, NFKB, TNF-α, IL1B, IL6, LGALS, and CATH1), antioxidant (SOD1, CAT, GPX1, GST, Nrf2, Keap1, HMOX1, and NQO1), and GIT health (CALB1, GT, and MUC2) genes in healthy and diarrheic lambs.</p><p><strong>Results: </strong>Virulent genetic markers of pathogenic characteristics of E. coli (astA, Vt2e (Stx2e), CFA/I, groES and luxS) and Salmonella (invA, SopB, bcfC and avrA) were detected in all diarrheic lambs. PCR-DNA sequencing of immune, antioxidant and intestinal health genes found eleven single nucleotide polymorphisms (SNPs) linked to either diarrhea resistance or susceptibility in Barki lambs. Transcript levels of immune, antioxidant, and GIT health (CALB1, GT, and MUC2) genes varied between healthy and diarrheic lambs. Nucleotide sequence variation of the genes under inquiry between reference sequences in GenBank and those of the animals under investigation verified all identified SNPs. Significant (P = 0.001) erythrocytosis, neutrophilic leukocytosis, with lymphocytopenia were observed in diarrheic lambs. Significant (P = 0.001) increases in serum IL-1α, IL-1β, IL-6, TNF-α (90.5 ± 1.7, 101.8 ± 1.7, 72.3 ± 6.6, 71.26 ± 4.89 Pg/ml, respectively), serum Fb, Cp, Hp, SAA (230.7 ± 12.4 mg/dl, 6.5 ± 0.07 mg/dl, 2.5 ± 0.09 g/dl, 7.4 ± 0.4 mg/L, respectively), free radicals (MDA, NO), cortisol (6.91 ± 0.18 μg/dl) and growth hormone, with significant (P = 0.001) decreases in serum IL-10 (81.71 ± 1.05 Pg/ml), antioxidants (CAT, GPx), insulin, triiodothyronine (T3) and thyroxine
背景:尽管将腹泻描述为一种临床症状比描述为一种疾病更为准确。腹泻是绵羊医学中最重要的问题之一,尤其是对羔羊而言,由于其发病率和死亡率高、生长迟缓、兽医成本高,被认为是经济损失的主要来源。沙门氏菌和肠毒性大肠杆菌是导致腹泻的最常见、最具商业价值的病原体:本研究旨在监测腹泻羔羊的核苷酸序列变异、基因表达、血清炎症和氧化应激生物标志物。另一个目的是确定引起腹泻的沙门氏菌和大肠杆菌的不同病理类型和毒力基因:从 50 只腹泻羔羊和 50 只健康羔羊身上采集血液样本,然后分成 3 份,第一份加入 EDTA 进行全血细胞计数、DNA 和 RNA 提取。第二份样本中加入 5000 I.U. 的肝素钙,第三份样本则使用干净的普通试管。第二和第三部分离心提取血清和血浆,直至完成生化和免疫分析。收集粪便样本进行细菌学检查,并通过 PCR 分析鉴定细菌。对免疫(SELL、JAK2、SLC11A1、IL10、FEZF1、NCF4、LITAF、SBD2、NFKB、TNF-α、IL1B、IL6、LGALS 和 CATH1)进行 PCR-DNA 测序、健康羔羊和腹泻羔羊的抗氧化基因(SOD1、CAT、GPX1、GST、Nrf2、Keap1、HMOX1 和 NQO1)以及胃肠道健康基因(CALB1、GT 和 MUC2)。结果在所有腹泻羔羊中都检测到了大肠杆菌(astA、Vt2e (Stx2e)、CFA/I、groES 和 luxS)和沙门氏菌(invA、SopB、bcfC 和 avrA)的致病性病毒基因标记。免疫、抗氧化和肠道健康基因的 PCR-DNA 测序发现 11 个单核苷酸多态性(SNPs)与巴基羔羊的腹泻抵抗力或腹泻易感性有关。免疫、抗氧化和肠道健康(CALB1、GT和MUC2)基因的转录水平在健康羔羊和腹泻羔羊之间存在差异。所调查基因的核苷酸序列在 GenBank 中的参考序列与所调查动物的参考序列之间的变异验证了所有已确定的 SNPs。腹泻羔羊出现了明显的红细胞增多症、中性粒细胞增多症和淋巴细胞减少症(P = 0.001)。血清 IL-1α、IL-1β、IL-6、TNF-α(分别为 90.5 ± 1.7、101.8 ± 1.7、72.3 ± 6.6、71.26 ± 4.89 Pg/ml)、血清 Fb、Cp、Hp、SAA(分别为 230.7 ± 12.4 mg/dl、6.5 ± 0.07 mg/dl、2.5 ± 0.09 g/dl、7.4 ± 0.4 mg/L)、自由基(MDA、NO)、皮质醇(6.91 ± 0.18 μg/dl)和生长激素,腹泻羔羊血清IL-10(81.71 ± 1.05 Pg/ml)、抗氧化剂(CAT、GPx)、胰岛素、三碘甲状腺原氨酸(T3)和甲状腺素(T4)显著下降(P = 0.001):研究结果证实了以下理论:根据与炎症、抗氧化剂和肠道健康有关的基因 SNPs 选择羔羊,标记辅助选择(MAS)可用于预测和预防巴尔基绵羊腹泻。为了制定有效的管理方案并确定疾病发生的最易感风险期,还可以利用所研究基因的基因表达谱、促炎细胞因子和急性期蛋白作为羔羊肠炎的替代生物标记物。
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引用次数: 0
Morphological identification and distribution comparison of telocytes in pituitary gland between normal and cryptorchid yaks. 正常牦牛和隐睾牦牛垂体端细胞的形态鉴定和分布比较
IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES Pub Date : 2024-10-11 DOI: 10.1186/s12917-024-04307-1
Yumei Qi, Ligang Yuan, Jianlin Zeng, Xiaofen Wang, Long Ma, Jinghan Lv

Background: Telocytes (TCs) is a novel type of interstitial cells in many mammals organs, which participate in the organizational metabolism, mechanical support, immunomodulation and other aspects. The aim of this study was to explore the organizational chemical characteristics of TCs in pituitary gland and their changes in cryptorchid yaks.

Methods: Transmission electron microscopy (TEM), toluidine blue staining, immunofluorescence, qRT-PCR, and Western blotting may enable us to understand TCs distribution characteristics and biological functions.

Result: TEM confirmed the presence of TCs in the pituitary gland with small bodies and moniliform telopodes (Tps). The Tps extending out from the cell body to the peri-sinusoidal vessels spaces, the number of Tps is closely related to the morphology of the nucleus. The most obvious changes of TCs in the pituitary gland of cryptorchid yaks is the Tps are relatively shorter and decreased secretory vesicles. H.E. and toluidine blue staining revealed that TCs not only distributed between the sinusoidal blood vessels and the glandular cell clusters, but also present on the surface of vascular endothelial cells. The co-expression of TCs biomarkers, such as Vimentin/CD34, CD117/CD34 and α-SMA/CD34, were evaluated by immunofluorescence to further determine the phenotypic characteristics of TCs. Besides, we analyzed the mRNA and protein expression of these biomarkers to determine the characteristics of TCs changes and possible biological roles. Both the mRNA and protein expression of CD117 were significantly higher in the pituitary gland of cryptorchid yaks than in the normal (p < 0.01), the protein expression of CD34 in the cryptorchid yaks was significantly higher than the normal (p < 0.01). There were no significant difference in mRNA expression of Vimentin and α-SMA (p>0.05), while the protein expression were significantly increased in the normal yaks (p < 0.05).

Conclusions: In summary, this study reports for the first time that the biological characteristics of TCs in yak pituitary gland. Although there is no significant change in the distribution characteristics, the changes in biological features of TCs in cryptorchid yaks are clear, suggesting that TCs participated in alteration in the local microenvironment of the pituitary gland. Therefore, our study provides clues for further investigating the role of TCs in the pituitary gland during the occurrence of cryptorchidism in yaks.

背景:端细胞(TCs)是哺乳动物许多器官中的一种新型间质细胞,参与组织代谢、机械支持、免疫调节等方面。本研究旨在探讨隐睾牦牛垂体TCs的组织化学特征及其变化:方法:通过透射电子显微镜(TEM)、甲苯胺蓝染色、免疫荧光、qRT-PCR和Western印迹等方法了解TCs的分布特征和生物学功能:结果:TEM证实垂体中存在TCs,它们具有小体和单形端粒(Tps)。Tps从细胞体延伸到窦周血管间隙,Tps的数量与细胞核的形态密切相关。隐睾牦牛垂体TC最明显的变化是Tps相对变短,分泌泡减少。H.E.和甲苯胺蓝染色显示,TCs不仅分布在窦状血管和腺细胞簇之间,还存在于血管内皮细胞表面。免疫荧光评估了TCs生物标记物的共表达,如Vimentin/CD34、CD117/CD34和α-SMA/CD34,以进一步确定TCs的表型特征。此外,我们还分析了这些生物标志物的 mRNA 和蛋白表达,以确定 TCs 的变化特征和可能的生物学作用。综上所述,本研究首次报道了牦牛垂体中TCs的生物学特征。虽然分布特征没有明显变化,但隐睾牦牛TCs生物学特征的变化非常明显,表明TCs参与了垂体局部微环境的改变。因此,我们的研究为进一步研究TCs在牦牛隐睾发生过程中在垂体中的作用提供了线索。
{"title":"Morphological identification and distribution comparison of telocytes in pituitary gland between normal and cryptorchid yaks.","authors":"Yumei Qi, Ligang Yuan, Jianlin Zeng, Xiaofen Wang, Long Ma, Jinghan Lv","doi":"10.1186/s12917-024-04307-1","DOIUrl":"10.1186/s12917-024-04307-1","url":null,"abstract":"<p><strong>Background: </strong>Telocytes (TCs) is a novel type of interstitial cells in many mammals organs, which participate in the organizational metabolism, mechanical support, immunomodulation and other aspects. The aim of this study was to explore the organizational chemical characteristics of TCs in pituitary gland and their changes in cryptorchid yaks.</p><p><strong>Methods: </strong>Transmission electron microscopy (TEM), toluidine blue staining, immunofluorescence, qRT-PCR, and Western blotting may enable us to understand TCs distribution characteristics and biological functions.</p><p><strong>Result: </strong>TEM confirmed the presence of TCs in the pituitary gland with small bodies and moniliform telopodes (Tps). The Tps extending out from the cell body to the peri-sinusoidal vessels spaces, the number of Tps is closely related to the morphology of the nucleus. The most obvious changes of TCs in the pituitary gland of cryptorchid yaks is the Tps are relatively shorter and decreased secretory vesicles. H.E. and toluidine blue staining revealed that TCs not only distributed between the sinusoidal blood vessels and the glandular cell clusters, but also present on the surface of vascular endothelial cells. The co-expression of TCs biomarkers, such as Vimentin/CD34, CD117/CD34 and α-SMA/CD34, were evaluated by immunofluorescence to further determine the phenotypic characteristics of TCs. Besides, we analyzed the mRNA and protein expression of these biomarkers to determine the characteristics of TCs changes and possible biological roles. Both the mRNA and protein expression of CD117 were significantly higher in the pituitary gland of cryptorchid yaks than in the normal (p < 0.01), the protein expression of CD34 in the cryptorchid yaks was significantly higher than the normal (p < 0.01). There were no significant difference in mRNA expression of Vimentin and α-SMA (p>0.05), while the protein expression were significantly increased in the normal yaks (p < 0.05).</p><p><strong>Conclusions: </strong>In summary, this study reports for the first time that the biological characteristics of TCs in yak pituitary gland. Although there is no significant change in the distribution characteristics, the changes in biological features of TCs in cryptorchid yaks are clear, suggesting that TCs participated in alteration in the local microenvironment of the pituitary gland. Therefore, our study provides clues for further investigating the role of TCs in the pituitary gland during the occurrence of cryptorchidism in yaks.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11468414/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142406056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Variation in the gut microbiota during the early developmental stages of common carp (Cyprinus carpio L.) and its correlation with feed and pond water microflora. 鲤鱼(Cyprinus carpio L.)早期发育阶段肠道微生物群的变化及其与饲料和池水微生物群的相关性。
IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES Pub Date : 2024-10-11 DOI: 10.1186/s12917-024-04321-3
Jiahui Zhang, Yu Liu, Shijuan Shan, Cong Xu, Liguo An, Guiwen Yang, Lei Wang, Hua Li

Background: Fish gut microbiota undergo dynamic changes under the influence of many factors and play an important role in the nutrition, immunity and development in fish. Although common carp (Cyprinus carpio L.) is an economically important freshwater fish, there are few reports on its gut microbiota changes at different early developmental stages. In the present study, the gut microbiota of common carp during the early developmental stages and its correlation with the feed and pond water flora were studied using the Illumina MiSeq sequencing platform.

Results: The results showed that the gut microbiota of common carp underwent continuous and mild changes over the development process, and the pond water environment might provide bacterial resources and have a certain influence on the changes in the gut microbiota of common carp. However, host selection pressure played a more important role in shaping the gut microbiota. Although the gut microbiota was affected by many factors, the presence of core microbiota indicated that some bacterial species adapt to the gut microenvironment of common carp and played a role in its growth process.

Conclusions: The dynamic changes of gut microbiota of carp in early development stage were related to the feed, water environment and host selection. The results of this study provide a theoretical basis for healthy farming and disease prevention of common carp.

背景:鱼类肠道微生物群在多种因素的影响下发生动态变化,在鱼类的营养、免疫和发育过程中发挥着重要作用。虽然鲤鱼(Cyprinus carpio L.)是一种具有重要经济价值的淡水鱼,但有关其肠道微生物群在不同早期发育阶段变化的报道却很少。本研究利用 Illumina MiSeq 测序平台研究了鲤鱼早期发育阶段的肠道微生物群及其与饲料和池水菌群的相关性:结果表明,鲤鱼肠道微生物群在发育过程中发生了持续而温和的变化,池塘水环境可能提供了细菌资源,对鲤鱼肠道微生物群的变化有一定影响。然而,宿主的选择压力对肠道微生物群的形成起着更为重要的作用。虽然鲤鱼肠道微生物群受到多种因素的影响,但核心微生物群的存在表明一些细菌种类适应了鲤鱼的肠道微环境,并在其生长过程中发挥了作用:结论:鲤鱼肠道微生物群在发育早期的动态变化与饲料、水环境和宿主选择有关。本研究结果为鲤鱼的健康养殖和疾病预防提供了理论依据。
{"title":"Variation in the gut microbiota during the early developmental stages of common carp (Cyprinus carpio L.) and its correlation with feed and pond water microflora.","authors":"Jiahui Zhang, Yu Liu, Shijuan Shan, Cong Xu, Liguo An, Guiwen Yang, Lei Wang, Hua Li","doi":"10.1186/s12917-024-04321-3","DOIUrl":"10.1186/s12917-024-04321-3","url":null,"abstract":"<p><strong>Background: </strong>Fish gut microbiota undergo dynamic changes under the influence of many factors and play an important role in the nutrition, immunity and development in fish. Although common carp (Cyprinus carpio L.) is an economically important freshwater fish, there are few reports on its gut microbiota changes at different early developmental stages. In the present study, the gut microbiota of common carp during the early developmental stages and its correlation with the feed and pond water flora were studied using the Illumina MiSeq sequencing platform.</p><p><strong>Results: </strong>The results showed that the gut microbiota of common carp underwent continuous and mild changes over the development process, and the pond water environment might provide bacterial resources and have a certain influence on the changes in the gut microbiota of common carp. However, host selection pressure played a more important role in shaping the gut microbiota. Although the gut microbiota was affected by many factors, the presence of core microbiota indicated that some bacterial species adapt to the gut microenvironment of common carp and played a role in its growth process.</p><p><strong>Conclusions: </strong>The dynamic changes of gut microbiota of carp in early development stage were related to the feed, water environment and host selection. The results of this study provide a theoretical basis for healthy farming and disease prevention of common carp.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11468302/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142406058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of productive performance of Marandi, White Leghorn, and Marandi-White Leghorn crossbred chickens. 比较马兰地鸡、白莱格鸡和马兰地-白莱格鸡杂交鸡的生产性能。
IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES Pub Date : 2024-10-10 DOI: 10.1186/s12917-024-04314-2
Somayeh Pourhamidi, Ali Esmailizadeh, Mohamad Salarmoini, Masood Asadi Fozi

Background: Recently, efforts like crossbreeding, strain crossing, and inbred hybridization have been performed to improve productive performance in native chickens. This experiment was carried out to evaluate the effects of crossbreeding on the improvement of the productivity of a native chicken. The White Leghorn exotic chicken (WL) as dam line and the Marandi indigenous chicken (MA) as sire line were used to generate the crossbreds (MA × WL). Body weight and egg production traits were measured in the three groups.

Results: There are significant performance differences between WL and MA, with WL exhibiting superior body weight and egg production compared to MA (P < 0.05). Crossbreeding between WL and MA significantly improved most traits, with MA × WL outperforming MA. Negative heterosis was observed for body weight showing similarities between MA and MA × WL. Notably, MA × WL showed a closer resemblance to WL than MA in hen-day egg production at 40 and 44 weeks (P < 0.05).

Conclusions: The results derived from this study show that the economic performance of native chickens can be enhanced through crossbreeding with exotic chickens. Using the crossbred chickens in rural areas can be more efficient than the native chickens. In addition, the resilience of the native chickens to the local environment can be transmitted genetically to the crossbred which should be investigated in further studies.

背景:最近,为了提高土鸡的生产性能,人们进行了杂交、品系杂交和近交杂交等努力。本实验旨在评估杂交对提高本地鸡生产性能的影响。以白莱格霍恩外来鸡(WL)为母本品系,马兰地本地鸡(MA)为父本品系,产生杂交种(MA × WL)。对三组鸡的体重和产蛋性状进行了测定:结果:WL 和 MA 之间存在明显的性能差异,WL 的体重和产蛋量均优于 MA(P 结论:WL 和 MA 之间存在明显的性能差异,WL 的体重和产蛋量均优于 MA(P):本研究的结果表明,通过与外来鸡杂交,可以提高本地鸡的经济效益。在农村地区使用杂交鸡比本地鸡更有效率。此外,本地鸡对当地环境的适应能力可通过基因传递给杂交鸡,这一点应在进一步研究中加以探讨。
{"title":"Comparison of productive performance of Marandi, White Leghorn, and Marandi-White Leghorn crossbred chickens.","authors":"Somayeh Pourhamidi, Ali Esmailizadeh, Mohamad Salarmoini, Masood Asadi Fozi","doi":"10.1186/s12917-024-04314-2","DOIUrl":"10.1186/s12917-024-04314-2","url":null,"abstract":"<p><strong>Background: </strong>Recently, efforts like crossbreeding, strain crossing, and inbred hybridization have been performed to improve productive performance in native chickens. This experiment was carried out to evaluate the effects of crossbreeding on the improvement of the productivity of a native chicken. The White Leghorn exotic chicken (WL) as dam line and the Marandi indigenous chicken (MA) as sire line were used to generate the crossbreds (MA × WL). Body weight and egg production traits were measured in the three groups.</p><p><strong>Results: </strong>There are significant performance differences between WL and MA, with WL exhibiting superior body weight and egg production compared to MA (P < 0.05). Crossbreeding between WL and MA significantly improved most traits, with MA × WL outperforming MA. Negative heterosis was observed for body weight showing similarities between MA and MA × WL. Notably, MA × WL showed a closer resemblance to WL than MA in hen-day egg production at 40 and 44 weeks (P < 0.05).</p><p><strong>Conclusions: </strong>The results derived from this study show that the economic performance of native chickens can be enhanced through crossbreeding with exotic chickens. Using the crossbred chickens in rural areas can be more efficient than the native chickens. In addition, the resilience of the native chickens to the local environment can be transmitted genetically to the crossbred which should be investigated in further studies.</p>","PeriodicalId":9041,"journal":{"name":"BMC Veterinary Research","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11466034/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142399286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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BMC Veterinary Research
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