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Insight into molecular phylogeny and haplotype diversity of the dog louse fly (Hippobosca longipennis) in India. 印度犬虱蝇分子系统发育及单倍型多样性研究。
IF 2.6 2区 农林科学 Q1 VETERINARY SCIENCES Pub Date : 2026-02-07 DOI: 10.1186/s12917-026-05356-4
Vikas Dhankhar, Anil K Nehra, Aman D Moudgil
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引用次数: 0
Seroprevalence of rift valley fever virus and associated risk factors in small ruminants at human-livestock-wildlife interface within Uganda's conservation areas. 乌干达自然保护区人类-牲畜-野生动物界面小反刍动物中裂谷热病毒的血清流行率及相关危险因素
IF 2.6 2区 农林科学 Q1 VETERINARY SCIENCES Pub Date : 2026-02-07 DOI: 10.1186/s12917-026-05332-y
Phiona Katushabe, Dennison Kizito, Charity Angella Nassuna, Joseph Kikwabanga Mutyaba, Swaib A Lule, Gladys Kiggundu Nakanjako, Nackson Babi, Wilber Ssembajjwe, Tonny Kayizi, Milton Bahati, Martin Esau, Stella Atim, Brian Kivumbi, Teddy Nakayiki Muwawu, Deogratius Nteza, Eric Morris Enyel, Alice Namatovu, Stephen Balinandi, Musa Sekamatte, Gladys Kalema-Zikusoka, Patrick Atimnedi, Julius J Lutwama, Luke Nyakarahuka, Deo B Ndumu
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引用次数: 0
Dietary capsaicin supplementation attenuates jejunal injury of piglets challenged with lipopolysaccharide. 饲粮中添加辣椒素可减轻脂多糖攻毒仔猪空肠损伤。
IF 2.6 2区 农林科学 Q1 VETERINARY SCIENCES Pub Date : 2026-02-07 DOI: 10.1186/s12917-026-05346-6
Zechen Xie, Wenyi Liu, Xin Zhang, Hao Zhang, Jianlei Zhao, Fengchao Wang, Li Pang, Tian Wang, Lili Zhang
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引用次数: 0
Lower prevalence of obesity and nutritional imbalances in dogs fed a raw meat-based diet (RMBD) compared to a commercial complete diet. 与商业完整饮食相比,以生肉为基础的饮食(RMBD)的狗肥胖和营养不平衡的患病率较低。
IF 2.6 2区 农林科学 Q1 VETERINARY SCIENCES Pub Date : 2026-02-06 DOI: 10.1186/s12917-026-05283-4
Lara M von Lindeiner, Simon F Müller, Elisabeth Müller, Romy M Heilmann, Ingrid Vervuert

Background: Canine obesity is a significant health concern in spite of current feeding trends claiming healthier diets. Among these trends, raw meat-based diets (RMBD) have gained in popularity. Scientific evidence supporting these claims is limited, and nutritional imbalances are of concern in RMBD-fed dogs. This study aimed to assess the impact of RMBD-feeding on body condition in dogs in comparison to feeding a commercial complete diet (CD). A total of 104 healthy dogs were included, of which 51 dogs (age median: 4.1 years, interquartile range [IQR]: 3.2-5.9 years; body weight (BW) median: 24.8 kg, IQR: 16.3-31.9 kg) were fed an RMBD and 53 dogs (age median: 4.8 years, IQR: 3.3-5.8 years; BW median: 25.4 kg, IQR: 18.9-27.7 kg) received a CD, both for at least 12 months prior to enrollment. Enrolled dogs underwent two clinical evaluations 3-5 months apart. During these evaluations, blood, urine, and fecal samples were collected, and the patient and diet history was obtained.

Results: RMBD-fed dogs had lower body condition scores (BCS; median: 5, IQR: 4-5) compared to CD-fed dogs (median: 6, IQR: 5-7, P < 0.001). Energy intake was lower with RMBD rations (median coverage of the daily recommended metabolizable energy [ME]: 89%) compared to CD rations (median coverage of the daily recommended ME: 102%, P = 0.015). Coverage of the daily energy intake was inversely correlated with BCS. In RMBD rations, the calcium (Ca): phosphorus (P) ratio (median: 1.0), failed to meet the recommended ratio of 1.4 and was lower than with CD rations (median: 1.4, P < 0.001); estimated intakes of Ca (P < 0.001), P (P < 0.001), sodium (P < 0.001) and magnesium (P = 0.004) were lower than in CD rations but close to the recommendations. Estimated intakes of Ca, P, and sodium in CD rations were at least twice the recommended amounts. Blood serum analysis revealed lower serum iodine (P = 0.001), copper (P = 0.005), zinc (P < 0.001), and manganese (P = 0.035) concentrations in RMBD-fed dogs than in CD-fed dogs.

Conclusion: While RMBD-feeding might offer the advantage of a leaner body condition, concerns about nutritional imbalances warrant further investigation, even though RMBD-fed dogs do not show clinical signs of nutrient deficiencies.

背景:犬类肥胖是一个重要的健康问题,尽管目前的喂养趋势声称更健康的饮食。在这些趋势中,生肉饮食(RMBD)越来越受欢迎。支持这些说法的科学证据有限,而且人民币喂养的狗的营养失衡令人担忧。本研究旨在评估饲喂人民币d与饲喂商业全饲粮(CD)对狗体状况的影响。共纳入104只健康犬,其中51只犬(年龄中位数:4.1岁,四分位间距[IQR]: 3.2-5.9岁;体重(BW)中位数:24.8 kg, IQR: 16.3-31.9 kg)被喂食人民币d; 53只犬(年龄中位数:4.8岁,IQR: 3.3-5.8岁;体重中位数:25.4 kg, IQR: 18.9-27.7 kg)被喂食CD,均在入组前至少12个月。入选的狗每隔3-5个月进行两次临床评估。在这些评估中,收集了血液、尿液和粪便样本,并获得了患者和饮食史。结果:与cd喂养的狗(中位数:6,IQR: 5-7, P)相比,rmbd喂养的狗的身体状况评分(BCS;中位数:5,IQR: 4-5)较低。结论:虽然rmbd喂养可能提供更瘦的身体状况的优势,但对营养失衡的担忧值得进一步研究,即使rmbd喂养的狗没有表现出营养缺乏的临床症状。
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引用次数: 0
Whole genome sequencing in animal health: applications, challenges, and future directions. 全基因组测序在动物健康中的应用、挑战和未来方向。
IF 2.6 2区 农林科学 Q1 VETERINARY SCIENCES Pub Date : 2026-02-06 DOI: 10.1186/s12917-026-05329-7
Johannes Charlier, Alistair Antonopoulos, Bart J G Broeckx, Aaron Pomerantz, Veronica L Fowler, Volodimir Olexiouk, Sebastiaan Theuns, Sven Arnouts
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引用次数: 0
Non-contact detection of post-regurgitation deep inhalation in calves using infrared thermography and deep learning-based nostril segmentation. 利用红外热像仪和基于深度学习的鼻孔分割非接触检测犊牛反流后深度吸入。
IF 2.6 2区 农林科学 Q1 VETERINARY SCIENCES Pub Date : 2026-02-06 DOI: 10.1186/s12917-026-05340-y
Sueun Kim, Norio Yamagishi, Shingo Ishikawa, Shinobu Tsuchiaka
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引用次数: 0
RNA-seq evaluation of equine alveolar macrophages and monocyte-derived macrophages exposed to an inflammatory stimulus (short communication). 暴露于炎症刺激下的马肺泡巨噬细胞和单核细胞源性巨噬细胞的RNA-seq评估(短通信)。
IF 2.6 2区 农林科学 Q1 VETERINARY SCIENCES Pub Date : 2026-02-06 DOI: 10.1186/s12917-026-05322-0
Heng Kang, Gary K C Lee, Dorothee Bienzle, Jutta Hammermüller, Luis G Arroyo, Brandon N Lillie, Janet Beeler-Marfisi
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引用次数: 0
Kruppel like factor 12 gene regulates ovarian granulosa cells proliferation and apoptosis via the PI3K/Akt signaling pathway. Kruppel like factor 12基因通过PI3K/Akt信号通路调控卵巢颗粒细胞的增殖和凋亡。
IF 2.6 2区 农林科学 Q1 VETERINARY SCIENCES Pub Date : 2026-02-05 DOI: 10.1186/s12917-026-05336-8
Jiawei Cai, Bohao Zhao, Zhiyuan Bao, Yunpeng Li, Xiaoman Han, Yang Chen, Xinsheng Wu
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引用次数: 0
Exploration of the effect and mechanism of indirubin on infectious bovine rhinotracheitis virus based on network pharmacology. 基于网络药理学的吲哚红对传染性牛鼻气管炎病毒的作用及机制探讨。
IF 2.6 2区 农林科学 Q1 VETERINARY SCIENCES Pub Date : 2026-02-04 DOI: 10.1186/s12917-026-05325-x
Xuelian Ma, Heqi Lv, Xinbo Liu, Yingcai Ma, Qian Jiang, Jingqing Lv, Xin Li, Na Li, Qi Zhong, Gang Yao
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引用次数: 0
Molecular characterization and phylogeography of equine influenza virus H3N8 detected in donkeys in Nigeria 2022-2023. 2022-2023年尼日利亚驴中检测到的马流感病毒H3N8的分子特征和系统地理学
IF 2.6 2区 农林科学 Q1 VETERINARY SCIENCES Pub Date : 2026-02-03 DOI: 10.1186/s12917-025-05279-6
Nicodemus Mkpuma, Clement Meseko, Ismaila Shittu, Chukwu Chukwu, Felicitas Ngozi Afiukwa, Ifeanyichukwu Romanus Iroha, Maryam Muhammad, Ogbonnaya Ogbu

Background: Equine influenza virus (EIV) H3N8 is a highly contagious respiratory pathogen that poses significant health and economic risks to equids globally. In southeastern Nigeria where equids are sold and slaughtered, limited data exist on EIV epidemiology and circulating lineages.

Methods: To address this gap, an active surveillance was conducted between January 2022 and October 2023. A total of 400 nasal swabs were collected from horses and donkeys at slaughterhouses and animal markets. The swabs were screened for equine influenza virus (EIV) using quantitative Reverse-Transcription Polymerase Chain Reaction (RT-qPCR), and positive samples underwent whole-genome sequencing. A spatiotemporal Bayesian phylogeographic analysis was performed. Amino acid comparisons were carried out against the World Organization for Animal Health (WOAH) recommended Florida clade-1 (Fc-1) vaccine strains (accession numbers GU447312, DQ124192) and mutations were mapped onto 3D H3 hemagglutinin structure with protein data bank 4UO0 using PyMOL.

Results: Two samples (0.5%) from non clinical signs and deceased donkeys tested positive for the H3N8 virus. A spatiotemporal Bayesian phylogeographic analysis, which included sequences from outbreaks in Africa between 2018 and 2023, revealed multiple introductions of the virus into Africa. The introductions of Fc-1 lineage into Africa may have originated from Argentina (2018/2019) and the UK (2021), while Florida clade-2 seems to have originated from Ireland (2019). The 2022 H3N8 strains identified in this study may be a result of persistence from the 2018/2019 epizootic in northern Nigeria. Additionally, we discovered previously unreported hemagglutinin substitutions compared to the WOAH recommended Fc-1 vaccine strain, along with novel changes adjacent to antigenic sites and four distinct glycosylation profiles in the virus, which underscores their potential epidemiological significance.

Conclusions: Our findings revealed multiple introductions of EIV probably from South America and Western Europe, rapid virus evolution, and significant transboundary spread facilitated by livestock trade, particularly involving donkeys and subclinical infections in the transmission of the virus. These results underscore the persistence and evolution of EIV H3N8 (Fc-1) in Nigeria and emphasize the need for improved genomic surveillance, control measures, and vaccination strategies against EIV in Africa. Additionally, regulating transboundary livestock trade is essential to mitigate the risk of future outbreaks.

背景:马流感病毒(EIV) H3N8是一种高度传染性呼吸道病原体,对全球马类造成重大健康和经济风险。在出售和屠宰马的尼日利亚东南部,关于EIV流行病学和流行血统的数据有限。方法:为了解决这一差距,在2022年1月至2023年10月期间进行了主动监测。从屠宰场和动物市场的马和驴身上共收集了400份鼻拭子。使用定量逆转录聚合酶链式反应(RT-qPCR)对棉签进行马流感病毒(EIV)筛选,阳性样本进行全基因组测序。进行了时空贝叶斯系统地理分析。与世界动物卫生组织(World Organization for Animal Health, WOAH)推荐的Florida clade1 (Fc-1)疫苗株(加入号GU447312, DQ124192)进行氨基酸比较,并使用PyMOL将突变与蛋白数据库4UO0的3D H3血凝素结构进行比对。结果:来自无临床症状和死驴的两个样本(0.5%)检测出H3N8病毒阳性。一项时空贝叶斯系统地理分析,包括2018年至2023年非洲疫情的序列,揭示了该病毒多次传入非洲。将Fc-1谱系引入非洲可能起源于阿根廷(2018/2019)和英国(2021),而佛罗里达进化枝2似乎起源于爱尔兰(2019)。本研究中发现的2022年H3N8毒株可能是尼日利亚北部2018/2019年动物流行病持续存在的结果。此外,与世界卫生组织推荐的Fc-1疫苗株相比,我们发现了以前未报道的血凝素替代,以及病毒中抗原位点附近的新变化和四种不同的糖基化谱,这强调了它们潜在的流行病学意义。结论:我们的研究结果揭示了EIV的多次引入,可能来自南美和西欧,病毒快速进化,牲畜贸易促进了显著的跨界传播,特别是涉及驴和病毒传播的亚临床感染。这些结果强调了EIV H3N8 (Fc-1)在尼日利亚的持续存在和演变,并强调需要改进非洲针对EIV的基因组监测、控制措施和疫苗接种策略。此外,规范跨界牲畜贸易对于减轻未来暴发的风险至关重要。
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