The sequence of histopathological changes in hyperglycaemia-induced nephrotoxicity as well as alteration in renal parameters is still not well established in diabetic models. Hyperglycaemic ambience has been shown to generate oxidative stress which becomes a trigger for further degenerative changes that occur in the microenvironment of the kidney. This study was therefore intended to investigate histopathological alterations in vascular, glomerular and tubulointerstitial compartments of the renal tissues, and the corresponding changes in values of oxidative stress markers, creatinine clearance, proteinuria and serum creatinine concentration in a duration of three, seven and twelve weeks of sustained hyperglycaemia in diabetic. The experiment included four groups of adult Wistar rat, Group A (Normal Control, treated with normal saline), Groups B, C and D were induced with diabetes (treated with 65mg/kg body weight of streptozotocin) and allowed for 3 weeks 7 weeks and 12 weeks respectively. At termination, Oxidative stress markers were analyzed using Oxidative stress marker kits. A 24 hours urine collection was obtained from metabolic cages few hours before sacrifice and used for renal analysis and histopathological examination was done using a light microscope. Results reveals that oxidative stress was climaxed at 7th week and was maintained at a constant level while histopathological changes in glomerulus first presented on the 3rd week accompanied by vascular changes. Tubulointerstitial changes were noticed on the 7th week. On the 12th week renal parameters were significantly altered when compared to the animals sacrificed on 3th and 7th week. In conclusion, the sequence in diabetic–induced renal dysfunction begins with changes in vascular and glomerular compartment followed by distortion in tubulointerstitial compartment. An alteration in renal parameters presents lastly and correlates with the histopathological changes. These findings can be adopted in clinical management and treatment of diabetes-induced kidney dysfunction. J. Bio-Sci. 31(1): 29-37, 2023
{"title":"Study of Sequence of Histopathological Changes in Hyperglycaemia-Induced Experimental Diabetic Nephropathy in Wistar Rat Model","authors":"Fischer Christie Elum Elum, Agbor Cyril Abang Abang, Abireh Ifeanacho Ezeteonu Ezeteonu, Agaba Eric Agim Agim","doi":"10.3329/jbs.v31i1.69532","DOIUrl":"https://doi.org/10.3329/jbs.v31i1.69532","url":null,"abstract":"The sequence of histopathological changes in hyperglycaemia-induced nephrotoxicity as well as alteration in renal parameters is still not well established in diabetic models. Hyperglycaemic ambience has been shown to generate oxidative stress which becomes a trigger for further degenerative changes that occur in the microenvironment of the kidney. This study was therefore intended to investigate histopathological alterations in vascular, glomerular and tubulointerstitial compartments of the renal tissues, and the corresponding changes in values of oxidative stress markers, creatinine clearance, proteinuria and serum creatinine concentration in a duration of three, seven and twelve weeks of sustained hyperglycaemia in diabetic. The experiment included four groups of adult Wistar rat, Group A (Normal Control, treated with normal saline), Groups B, C and D were induced with diabetes (treated with 65mg/kg body weight of streptozotocin) and allowed for 3 weeks 7 weeks and 12 weeks respectively. At termination, Oxidative stress markers were analyzed using Oxidative stress marker kits. A 24 hours urine collection was obtained from metabolic cages few hours before sacrifice and used for renal analysis and histopathological examination was done using a light microscope. Results reveals that oxidative stress was climaxed at 7th week and was maintained at a constant level while histopathological changes in glomerulus first presented on the 3rd week accompanied by vascular changes. Tubulointerstitial changes were noticed on the 7th week. On the 12th week renal parameters were significantly altered when compared to the animals sacrificed on 3th and 7th week. In conclusion, the sequence in diabetic–induced renal dysfunction begins with changes in vascular and glomerular compartment followed by distortion in tubulointerstitial compartment. An alteration in renal parameters presents lastly and correlates with the histopathological changes. These findings can be adopted in clinical management and treatment of diabetes-induced kidney dysfunction. J. Bio-Sci. 31(1): 29-37, 2023","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"137 5","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135725159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mustafa Abul Kalam Azad Azad, Ahmad Humayan Kabir, Muhammad Nurul Amin Amin
A suitable micropropagation protocol and ex vitro acclimation method have been developed from in vitro grown seedling explants through cotyledonary node and leaf explants in consideration of the vegetable and medicinal properties of Greater Burdock. MS medium with 0.5-4.0 μM BAP showed highest percentage of axillary shoot regeneration from the cotyledonary nodal explants. Direct shoot regeneration was achieved by culturing 1.0 cm2 sections of about 25 days old leaves of in vitro grown shoot on MS medium enriched with 4.0 μM BAP and 2.0 μM IBA or NAA after 5 weeks of culture. Within six weeks of incubation on medium enriched with 4.0 M BAP and 2.0 M IBA or NAA, the leaf explants also developed callus from the cut margins. The greatest number of adventitious shoots could then be formed from the leaf-derived callus within 10 weeks of culture on the same media mix. More than 20 shoots were formed per callus clump at the third subculture, which had the highest rate of shoot multiplication. A. lappa's shoot and callus were both preserved at 5 ºC in MS medium with 4.0 μM Kn and 2.0 μM IBA, as well as 4.0 μM BAP and 2.0 μM IBA, respectively. The in vitro proliferated and elongated shoots were separated from callus clump for rooting. A root-induction MS medium with 6.0 μM IBA or NAA was used to cultivate the microshoots individually. All of the cultured microshoots generated 2-16 roots within 4 weeks of being moved to the rooting medium. Regenerated plantlets were transferred to vermiculite and successfully established in an ex vivo environment with a 98% survival rate. J. Bio-Sci. 31(1): 1-15, 2023
{"title":"In Vitro Regeneration, Conservation, and Field Evaluation of a Medicinal Plant– Greater Burdock (Arctium Lappa L.)","authors":"Mustafa Abul Kalam Azad Azad, Ahmad Humayan Kabir, Muhammad Nurul Amin Amin","doi":"10.3329/jbs.v31i1.69530","DOIUrl":"https://doi.org/10.3329/jbs.v31i1.69530","url":null,"abstract":"A suitable micropropagation protocol and ex vitro acclimation method have been developed from in vitro grown seedling explants through cotyledonary node and leaf explants in consideration of the vegetable and medicinal properties of Greater Burdock. MS medium with 0.5-4.0 μM BAP showed highest percentage of axillary shoot regeneration from the cotyledonary nodal explants. Direct shoot regeneration was achieved by culturing 1.0 cm2 sections of about 25 days old leaves of in vitro grown shoot on MS medium enriched with 4.0 μM BAP and 2.0 μM IBA or NAA after 5 weeks of culture. Within six weeks of incubation on medium enriched with 4.0 M BAP and 2.0 M IBA or NAA, the leaf explants also developed callus from the cut margins. The greatest number of adventitious shoots could then be formed from the leaf-derived callus within 10 weeks of culture on the same media mix. More than 20 shoots were formed per callus clump at the third subculture, which had the highest rate of shoot multiplication. A. lappa's shoot and callus were both preserved at 5 ºC in MS medium with 4.0 μM Kn and 2.0 μM IBA, as well as 4.0 μM BAP and 2.0 μM IBA, respectively. The in vitro proliferated and elongated shoots were separated from callus clump for rooting. A root-induction MS medium with 6.0 μM IBA or NAA was used to cultivate the microshoots individually. All of the cultured microshoots generated 2-16 roots within 4 weeks of being moved to the rooting medium. Regenerated plantlets were transferred to vermiculite and successfully established in an ex vivo environment with a 98% survival rate. J. Bio-Sci. 31(1): 1-15, 2023","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"6 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135724752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alimul Islam Islam, Nelufa Yasmin Yasmin, Nurul Islam Islam
Petroleum ether (pet. ether), chloroform (CHCl3) and methanol (CH3OH) extracts of Petunia integrifolia (Hook.) Schinz & Thell. Whole plant was subjected to assess cytotoxicity against the brine shrimp Artemia salina L. nauplii, larvicidal activity against the mosquito Culex quinquefasciatus Say larvae and piscicidal activity against the Poecilia reticulata Peters adults. The P. integrifolia extract in pet. ether showed the highest cytotoxic activity against A. salina nauplii by giving the minimum LC50 value of 49.25 parts per million (ppm) at 48 h, while in case of CHCl3 and CH3OH extracts, the values were 63.37 and 342.55 ppm, respectively. Against C. quinquefasciatus larvae the three extracts gave LC50 values of 4.92, 5.51 and171.26 ppm, respectively all after 48 h of exposure. Against P. reticulata the LC50 values were 21.70 ppm for pet. ether extract at 48 h, and 31.91 ppm for CHCl3 extract after 42 h of exposure but CH3OH extract offered no mortality. According to the intensity of activity, therefore, the extracts could be arranged in the descending order of: pet. ether > CHCl3 > CH3OH extracts; and the sensitivity of the test organisms to the extract of P. integrifolia could be arranged in the following descending order: C. quinquefasciatus larvae (in Pet. ether; LC50 = 4.92 ppm) > P. reticulata (in pet ether; LC50 = 21.70 ppm) >A. salina nauplii (in pet. ether; LC50 = 49.25 ppm) J. Bio-Sci. 31(1): 99-108, 2023
{"title":"Bioactive Potentials of Petunia Integrifolia (Hook.) Schinz & Thell. Against Culex Quinquefasciatus Larvae, Artemia Salina Nauplii and Poecilia Reticulata Adults","authors":"Alimul Islam Islam, Nelufa Yasmin Yasmin, Nurul Islam Islam","doi":"10.3329/jbs.v31i1.69538","DOIUrl":"https://doi.org/10.3329/jbs.v31i1.69538","url":null,"abstract":"Petroleum ether (pet. ether), chloroform (CHCl3) and methanol (CH3OH) extracts of Petunia integrifolia (Hook.) Schinz & Thell. Whole plant was subjected to assess cytotoxicity against the brine shrimp Artemia salina L. nauplii, larvicidal activity against the mosquito Culex quinquefasciatus Say larvae and piscicidal activity against the Poecilia reticulata Peters adults. The P. integrifolia extract in pet. ether showed the highest cytotoxic activity against A. salina nauplii by giving the minimum LC50 value of 49.25 parts per million (ppm) at 48 h, while in case of CHCl3 and CH3OH extracts, the values were 63.37 and 342.55 ppm, respectively. Against C. quinquefasciatus larvae the three extracts gave LC50 values of 4.92, 5.51 and171.26 ppm, respectively all after 48 h of exposure. Against P. reticulata the LC50 values were 21.70 ppm for pet. ether extract at 48 h, and 31.91 ppm for CHCl3 extract after 42 h of exposure but CH3OH extract offered no mortality. According to the intensity of activity, therefore, the extracts could be arranged in the descending order of: pet. ether > CHCl3 > CH3OH extracts; and the sensitivity of the test organisms to the extract of P. integrifolia could be arranged in the following descending order: C. quinquefasciatus larvae (in Pet. ether; LC50 = 4.92 ppm) > P. reticulata (in pet ether; LC50 = 21.70 ppm) >A. salina nauplii (in pet. ether; LC50 = 49.25 ppm) J. Bio-Sci. 31(1): 99-108, 2023","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"7 4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135724747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biofloc technology is a recent concept in Bangladesh, it has numerous advantages for enhancing aquaculture production in several countries, thereby contributing to achieve sustainable development goals. To identify the most suitable fish species for profitable commercial fish culture through Biofloc, a 100-day experiment was conducted at Pran Fisheries Project in Razapur Village, situated in Sreemangal Upazilla of Moulvibazar district, commencing in March 2021. The experiment comprised three treatments: Oreochromis niloticus, Anabus testudineus, and Labeo rohita with two replicates for each treatment. Water quality parameters were meticulously monitored to ensure optimal conditions. All treatments were conducted at a temperature of 26°C, with pH levels of 7.6-7.8, dissolved oxygen concentrations of 5-6 ppm. During the study, O. niloticus exhibited the highest body weight (66.23 ± 0.94) compared to A. testudineus (50.82 ± 0.94) and L. rohita (53.92 ± 0.17). Furthermore, L. rohita displayed a higher average daily weight gain (1.8 ± 0.003) and specific growth rate (3.29 ± 0.29). The best feed conversion ratio (FCR) was observed both for O. niloticus (0.98 ± 0.33) and A. testudineus (0.98 ± 0.03). In terms of survival rate, O. niloticus outperformed the other species, with a rate of 98%. However, O. niloticus demonstrated better aquaculture performance compared to the other species using biofloc technology. This research provides valuable insights in effective cultural techniques that can enhance fish production through biofloc technology, contributing to economic growth in aquaculture sector and national development. J. Bio-Sci. 31(1): 71-85, 2023
{"title":"Species Selection for Profitable Commercial Fish Culture Through Biofloc Technology","authors":"None Kamruzzaman, Taiba Akter Laboni Laboni, Mst Shahinur Khatun Khatun, Obaidur Rahman Rahman, Md Mahabubur Rahman Rahman, Nur E Farjana Ilah Ilah, Md Joynal Abedin Abedin, Md Ashekur Rahman Rahman, None Md Harun, Md Mahmudul Hasan Hasan, Md Akhtarul Islam Islam, Md Mizanur Rahman Rahman, Md Yeamin Hossain Hossain","doi":"10.3329/jbs.v31i1.69536","DOIUrl":"https://doi.org/10.3329/jbs.v31i1.69536","url":null,"abstract":"Biofloc technology is a recent concept in Bangladesh, it has numerous advantages for enhancing aquaculture production in several countries, thereby contributing to achieve sustainable development goals. To identify the most suitable fish species for profitable commercial fish culture through Biofloc, a 100-day experiment was conducted at Pran Fisheries Project in Razapur Village, situated in Sreemangal Upazilla of Moulvibazar district, commencing in March 2021. The experiment comprised three treatments: Oreochromis niloticus, Anabus testudineus, and Labeo rohita with two replicates for each treatment. Water quality parameters were meticulously monitored to ensure optimal conditions. All treatments were conducted at a temperature of 26°C, with pH levels of 7.6-7.8, dissolved oxygen concentrations of 5-6 ppm. During the study, O. niloticus exhibited the highest body weight (66.23 ± 0.94) compared to A. testudineus (50.82 ± 0.94) and L. rohita (53.92 ± 0.17). Furthermore, L. rohita displayed a higher average daily weight gain (1.8 ± 0.003) and specific growth rate (3.29 ± 0.29). The best feed conversion ratio (FCR) was observed both for O. niloticus (0.98 ± 0.33) and A. testudineus (0.98 ± 0.03). In terms of survival rate, O. niloticus outperformed the other species, with a rate of 98%. However, O. niloticus demonstrated better aquaculture performance compared to the other species using biofloc technology. This research provides valuable insights in effective cultural techniques that can enhance fish production through biofloc technology, contributing to economic growth in aquaculture sector and national development. J. Bio-Sci. 31(1): 71-85, 2023","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135724759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
An investigation was conducted to assess the effect of Parthenium hysterophorus L. leaf extracts on seedling growth of three wheat (Triticum aestivum L.) varieties viz. Sourav, BARI Gom 22 and BARI Gom 33. It was observed that the growth of wheat seedlings was significantly influenced by the Parthenium extract treatments. BARI Gom 22 showed more tolerance to Parthenium treatments than BARI Gom 33 and Sourav. Generally, wheat seedlings growth was inhibited by Parthenium extract treatments at all periods. The percent inhibition of shoot and root elongation were more visible at 5% treatment concentration at all periods than other extract treatments. On the other hand the number of root of wheat seedlings was not found to be affected by Parthenium at 7 and 10-day periods. It was also noted that the effect of Parthenium extracts on wheat seedling growth is concentrations dependent manner. J. Bio-Sci. 31(1): 61-70, 2023
{"title":"Assessment of Aqueous Leaf Extracts of Parthenium Hysterophorus L. On Seedlings Growth of Wheat","authors":"Nousin Akter Akter, Md Hasanur Rahman Rahman","doi":"10.3329/jbs.v31i1.69535","DOIUrl":"https://doi.org/10.3329/jbs.v31i1.69535","url":null,"abstract":"An investigation was conducted to assess the effect of Parthenium hysterophorus L. leaf extracts on seedling growth of three wheat (Triticum aestivum L.) varieties viz. Sourav, BARI Gom 22 and BARI Gom 33. It was observed that the growth of wheat seedlings was significantly influenced by the Parthenium extract treatments. BARI Gom 22 showed more tolerance to Parthenium treatments than BARI Gom 33 and Sourav. Generally, wheat seedlings growth was inhibited by Parthenium extract treatments at all periods. The percent inhibition of shoot and root elongation were more visible at 5% treatment concentration at all periods than other extract treatments. On the other hand the number of root of wheat seedlings was not found to be affected by Parthenium at 7 and 10-day periods. It was also noted that the effect of Parthenium extracts on wheat seedling growth is concentrations dependent manner. J. Bio-Sci. 31(1): 61-70, 2023","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"2 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135724766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Md Azizul Islam, Md Jawadul Haque, Md Mokter Hossain, SM Shahinul Islam
Prostate-Specific Antigen (PSA) is a prostate cancer tumor marker, but furthermore, studies have revealed that many people with benign prostatic issues or other conditions have high PSA levels in their blood. As a result, various approaches have been suggested to boost prostate-specific antigen specificity. The aim of the study to determine whether the free-to-total PSA ratio (percent fPSA) is able to be utilized to discriminate between prostate cancer and benign prostatic hyperplasia. From December 2018 to November 2022, 260 males with Lower Urinary Tract Symptoms (LUTS) symptoms attended Rajshahi Medical College Hospital in Bangladesh were recruited purposively for a cross sectional study as per inclusion criteria. Digital Rectal Examination (DRE) and transrectal ultrasonography were performed along with age analysis. Before any manipulation, total PSA, free PSA, and percent free PSA were assessed. Transrectal ultrasound-guided biopsy or digitally guided biopsy was used to confirm the diagnosis. SPSS 26 was used to analyze the data. Among the 260 individuals, 105 were diagnosed with prostate cancer and the remaining 155 were diagnosed with benign prostatic hyperplasia. Patients with CaP were much older than those with Benign Prostatic Hyperplasia (BPH) (Mean±SD was 72±4.4 vs. 64±6.6 years, respectively) (p = 0.00001). The sensitivity, specificity, and positive predictive value of % free PSA (at cut off point 0.16) were greater (94%, 93%, 90% respectively) than those of total PSA (84%, 48%, 52% respectively). The proportion of false positives was lower in percent free PSA (10%) than in tPSA (19%). All of these considerations are critical in establishing the appropriateness of a screening test. In case of percent free PSA, the overall accuracy was 93%, significantly higher than tPSA (62%) (p<0.005). Thus, the free/total/ serum PSA ratio assists more accurately in differentiating BPH from prostate cancer. When compared to tPSA, the fPSA/tPSA and ratio enhance the discrimination between BPH and CaP comparable and are equally beneficial in lowering the incidence of unnecessary biopsies, but tPSA alone has less impact. J. Bio-Sci. 30(2): 67-77, 2022
{"title":"Prospective Analysis of Percent Free Prostate-Specific Antigen as a Better Predictor of Early Diagnosis of Prostate Cancer","authors":"Md Azizul Islam, Md Jawadul Haque, Md Mokter Hossain, SM Shahinul Islam","doi":"10.3329/jbs.v30i2.68914","DOIUrl":"https://doi.org/10.3329/jbs.v30i2.68914","url":null,"abstract":"Prostate-Specific Antigen (PSA) is a prostate cancer tumor marker, but furthermore, studies have revealed that many people with benign prostatic issues or other conditions have high PSA levels in their blood. As a result, various approaches have been suggested to boost prostate-specific antigen specificity. The aim of the study to determine whether the free-to-total PSA ratio (percent fPSA) is able to be utilized to discriminate between prostate cancer and benign prostatic hyperplasia. From December 2018 to November 2022, 260 males with Lower Urinary Tract Symptoms (LUTS) symptoms attended Rajshahi Medical College Hospital in Bangladesh were recruited purposively for a cross sectional study as per inclusion criteria. Digital Rectal Examination (DRE) and transrectal ultrasonography were performed along with age analysis. Before any manipulation, total PSA, free PSA, and percent free PSA were assessed. Transrectal ultrasound-guided biopsy or digitally guided biopsy was used to confirm the diagnosis. SPSS 26 was used to analyze the data. Among the 260 individuals, 105 were diagnosed with prostate cancer and the remaining 155 were diagnosed with benign prostatic hyperplasia. Patients with CaP were much older than those with Benign Prostatic Hyperplasia (BPH) (Mean±SD was 72±4.4 vs. 64±6.6 years, respectively) (p = 0.00001). The sensitivity, specificity, and positive predictive value of % free PSA (at cut off point 0.16) were greater (94%, 93%, 90% respectively) than those of total PSA (84%, 48%, 52% respectively). The proportion of false positives was lower in percent free PSA (10%) than in tPSA (19%). All of these considerations are critical in establishing the appropriateness of a screening test. In case of percent free PSA, the overall accuracy was 93%, significantly higher than tPSA (62%) (p<0.005). Thus, the free/total/ serum PSA ratio assists more accurately in differentiating BPH from prostate cancer. When compared to tPSA, the fPSA/tPSA and ratio enhance the discrimination between BPH and CaP comparable and are equally beneficial in lowering the incidence of unnecessary biopsies, but tPSA alone has less impact. J. Bio-Sci. 30(2): 67-77, 2022","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135816894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Crotalaria L. is a genus of flowering plants in the family Fabaceae (subfamily Faboideae). Mitotic index is proportionately relevant to cell division rate; mitotic index was found higher (10.38±1.08%) in C. spectabilis and lower (9.43±0.304%) in C. juncea. The nuclear organizations were observed chromocentric in all examined species. The interphase chromosome volume was highest in C. juncea (0.596±0.27 µ³ in meristematic cell and 0.408±0.21 µ³ in differentiated cell) and lowest in C. saltiana (0.205±0.31 µ³ in meristematic cell and 0.16±0.25 µ³ in differentiated cell) which do not correlated with the somatic chromosome number but may be related with environmental condition. The largest cell size was found in C. juncea (20.47±0.49 µm2 in meristematic cell and 106.47±0.63 µm2 in differentiated cell) and the smallest in C. saltiana (14.43±0.39 µm2 in meristematic cell and 45.86±0.21 µm2 in differentiated cell). It was also observed that, the mean value of cell size was larger in differentiated cell compared to meristematic cell in all the studied species of Crotalaria. Chromocenter numbers were found highest (13.66±0.14) in C. saltiana and lowest (9.50±0.32) in C. juncea which are considered as more primitive and more advance in nature, respectively. Percentages of heterochromatin per nuclear area were found highest (14.75±0.71) in C. saltiana and lowest (9.67±0.71) in C. juncea which are considered as more primitive and more advance in nature, respectively. J. Bio-Sci. 30(2): 79-88, 2022
{"title":"Nuclear Phenotype and Heterochromatin Percentages in Somatic Cells on Five Species of Crotalaria L.","authors":"Md Mamunur Rashid Sarkar, Md Saifur Rahman, Rezaul Karim, Golam Kabir","doi":"10.3329/jbs.v30i2.68915","DOIUrl":"https://doi.org/10.3329/jbs.v30i2.68915","url":null,"abstract":"Crotalaria L. is a genus of flowering plants in the family Fabaceae (subfamily Faboideae). Mitotic index is proportionately relevant to cell division rate; mitotic index was found higher (10.38±1.08%) in C. spectabilis and lower (9.43±0.304%) in C. juncea. The nuclear organizations were observed chromocentric in all examined species. The interphase chromosome volume was highest in C. juncea (0.596±0.27 µ³ in meristematic cell and 0.408±0.21 µ³ in differentiated cell) and lowest in C. saltiana (0.205±0.31 µ³ in meristematic cell and 0.16±0.25 µ³ in differentiated cell) which do not correlated with the somatic chromosome number but may be related with environmental condition. The largest cell size was found in C. juncea (20.47±0.49 µm2 in meristematic cell and 106.47±0.63 µm2 in differentiated cell) and the smallest in C. saltiana (14.43±0.39 µm2 in meristematic cell and 45.86±0.21 µm2 in differentiated cell). It was also observed that, the mean value of cell size was larger in differentiated cell compared to meristematic cell in all the studied species of Crotalaria. Chromocenter numbers were found highest (13.66±0.14) in C. saltiana and lowest (9.50±0.32) in C. juncea which are considered as more primitive and more advance in nature, respectively. Percentages of heterochromatin per nuclear area were found highest (14.75±0.71) in C. saltiana and lowest (9.67±0.71) in C. juncea which are considered as more primitive and more advance in nature, respectively. J. Bio-Sci. 30(2): 79-88, 2022","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135817037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bakul Bhattacharjee, Md Ekhlas Hossain, Mst Naznin Ayesha, SM Shahinul Islam
Dendrobium is a sympodial, epiphytic orchid that belongs to the family of Orchidaceae. Bangladesh has a total of 188 different species under 78 genera of this tribe. Among them, there are 27 species of Dendrobium. Considering the potential value, the 16 species of Dendrobium are especially highlighted for herbal, horticultural, and ornamental purposes. They are also valuable for flowering as pot plants or hanging baskets. Dendrobium orchids are highly important in economically, socially, culturally, and as medicinal orchids. The basic needs of humans, the morphological characteristics of these orchids, such as the shape of pseudobulb, leaf, variation of inflorescence, floral characteristics, and their biodiversity evaluation in Bangladesh, are very important, and have been taken up for the present study. Besides these, ruthless collection by people who are thirsty for beauty has made the species endangered. On the other hand, the species has lost its habitat due to the removal of large trees. So, this research program has been conducted to save these valuable species from extinction. In addition, by studying their morphology, scientists and researchers gain insights into the evolutionary adaptations, reproductive strategies, and ecological interactions of orchids. For the present study exist and endangered Dendrobium about 2-3 years old and flower bearing matured plants were studied for development of morphological characterization of these orchids were flourished. J. Bio-Sci. 30(2): 47-57, 2022
{"title":"Morphological Diversity Analysis of Some Existing and Endangered Dendrobium Orchid Species in Bangladesh","authors":"Bakul Bhattacharjee, Md Ekhlas Hossain, Mst Naznin Ayesha, SM Shahinul Islam","doi":"10.3329/jbs.v30i2.68911","DOIUrl":"https://doi.org/10.3329/jbs.v30i2.68911","url":null,"abstract":"Dendrobium is a sympodial, epiphytic orchid that belongs to the family of Orchidaceae. Bangladesh has a total of 188 different species under 78 genera of this tribe. Among them, there are 27 species of Dendrobium. Considering the potential value, the 16 species of Dendrobium are especially highlighted for herbal, horticultural, and ornamental purposes. They are also valuable for flowering as pot plants or hanging baskets. Dendrobium orchids are highly important in economically, socially, culturally, and as medicinal orchids. The basic needs of humans, the morphological characteristics of these orchids, such as the shape of pseudobulb, leaf, variation of inflorescence, floral characteristics, and their biodiversity evaluation in Bangladesh, are very important, and have been taken up for the present study. Besides these, ruthless collection by people who are thirsty for beauty has made the species endangered. On the other hand, the species has lost its habitat due to the removal of large trees. So, this research program has been conducted to save these valuable species from extinction. In addition, by studying their morphology, scientists and researchers gain insights into the evolutionary adaptations, reproductive strategies, and ecological interactions of orchids. For the present study exist and endangered Dendrobium about 2-3 years old and flower bearing matured plants were studied for development of morphological characterization of these orchids were flourished. J. Bio-Sci. 30(2): 47-57, 2022","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"70 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135817040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Md Nahid Islam Chowdhury, Md Anayet Ullah, Md Belal Hossain, S M Shahinul Islam
The most common congenital anomaly and a major contributor to infant and toddler mortality is congenital heart disease (CHD). Although it has high mortality rate but majority of the cases are asymptomatic during early life and usually discovered during routine checkup. To determine the incidence and pattern of congenital heart disease in children in a tertiary care hospital of Bangladesh. This cross-sectional study was conducted among children aged 1 day to 10 years old with cardiac problems who were hospitalized to the Paediatric department of Rajshahi Medical College Hospital, Rajshahi, Bangladesh from August 2018 to August 2021. Early detection of CHD is possible by thoroughly analyzing heart murmurs, clinical manifestations, and outcomes. The data were analyzed utilizing IBM SPSS (version-23). A total of 3512 children under the age of 10 years were evaluated. Here 1.54% of the patients had a CHD murmur. In this study, the most common type of congenital heart illness was a ventricular septal defect (VSD) (37.05%). Atrial septal defect (ASD) (24.07%) was found second most common CHD. Rapid breathing (63%) and poor growth (44.4%) were major clinical presentation. According to types of murmur among patients, 27(50%) had pansystolic murmur, 15(27.77%) had ejection systolic murmur and 12(22.23%) had continuous murmur. Congenital heart disease is a major health concern for Paediatricians due to its significant health hazards and high mortality rate. Early identification of congenital cardiac disease is crucial for preventing complications, lowering mortality, and for proper therapy. J. Bio-Sci. 30(2): 39-45, 2022
{"title":"Pattern of Congenital Heart Diseases in Children in a Tertiary Care Hospital of Bangladesh","authors":"Md Nahid Islam Chowdhury, Md Anayet Ullah, Md Belal Hossain, S M Shahinul Islam","doi":"10.3329/jbs.v30i2.68910","DOIUrl":"https://doi.org/10.3329/jbs.v30i2.68910","url":null,"abstract":"The most common congenital anomaly and a major contributor to infant and toddler mortality is congenital heart disease (CHD). Although it has high mortality rate but majority of the cases are asymptomatic during early life and usually discovered during routine checkup. To determine the incidence and pattern of congenital heart disease in children in a tertiary care hospital of Bangladesh. This cross-sectional study was conducted among children aged 1 day to 10 years old with cardiac problems who were hospitalized to the Paediatric department of Rajshahi Medical College Hospital, Rajshahi, Bangladesh from August 2018 to August 2021. Early detection of CHD is possible by thoroughly analyzing heart murmurs, clinical manifestations, and outcomes. The data were analyzed utilizing IBM SPSS (version-23). A total of 3512 children under the age of 10 years were evaluated. Here 1.54% of the patients had a CHD murmur. In this study, the most common type of congenital heart illness was a ventricular septal defect (VSD) (37.05%). Atrial septal defect (ASD) (24.07%) was found second most common CHD. Rapid breathing (63%) and poor growth (44.4%) were major clinical presentation. According to types of murmur among patients, 27(50%) had pansystolic murmur, 15(27.77%) had ejection systolic murmur and 12(22.23%) had continuous murmur. Congenital heart disease is a major health concern for Paediatricians due to its significant health hazards and high mortality rate. Early identification of congenital cardiac disease is crucial for preventing complications, lowering mortality, and for proper therapy. J. Bio-Sci. 30(2): 39-45, 2022","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135816892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The chemical mutation is one of the important factors in the enrichment of the salt resistance capacity of the rhizobium isolates. Therefore, this study focused on 10 isolates of rhizobium collected from soil samples of Babile, Hararghe region, Ethiopia. All the collected isolates turned into a moderately yellow, yellow, and deep yellow color in yeast extract mannitol agar medium (YEMA) containing bromothymol blue after 48 h of incubation. It indicated that all the isolates were acid-producing Rhizobia. Moreover, based on colony morphology and diameter, 70% of the isolates displayed large mucoid colonies, and 30% of the isolates showed large watery colonies in YEMA media. Among the 10 isolates, 9(90%), 9(90%), 6(60%), 6(60%), 2(20%), and 1(10%), were grown at different salinity levels such as 2%, 4%, 6%, 8%, 9% and 10% of NaCl, correspondingly. The most salt-resistant wild isolate was HUCR 6 collected from Babile soil grown at 10% NaCl salinity level. A total of six mutants were considered after chemical mutagenesis based on their capacity to survive in the extreme salinity levels (11 to 14%). Mutant isolates such as HUCRM 4, HUCRM 5, and HUCRM 6 were the most tolerant Rhizobium that grew at the salinity level of 11 to 14% NaCl. The most sensitive mutant isolate was HUCRM 10 followed by the isolates HUCRM 8 and HUCRM 9 was the next sensitive mutant Rhizobia that grew only at 11% of NaCl concentration. Compared to the Rhizobium wild isolates, the mutant isolates were observed to be more tolerant to a medium containing higher concentration of NaCl, as high as 11% to 14%. Besides, 80% of the mutant isolates demonstrated effective nodulation with the common beans. The mutant isolate (HUCRM 4) showed better performance in relation to root nodule performance of Rhizobium species and increased the plant biomass production. In this study, mutant isolates HUCRM 6, which is tolerated to 14% NaCl, and HUCRM 4, HUCRM 5, and HUCRM 6 isolates tolerated at 12% salinity level. Finally, based on their symbiotic efficiency and tolerance to extreme salt levels, these mutant isolates (HUCRM 4, HUCRM 5 and HUCRM 6) were encouraged to be used for the development of Rhizobium inoculants of common beans grown under extreme saline conditions. J. Bio-Sci. 30(2): 13-22, 2022
{"title":"Effect of Chemical Mutagenesis on Salt Tolerance of Common Beans (Phaseolus vulgaris L.) Nodulating Rhizobium","authors":"Mulugeta Mekonnen, Ameha Kebede, Manikandan Muthuswamy","doi":"10.3329/jbs.v30i2.68906","DOIUrl":"https://doi.org/10.3329/jbs.v30i2.68906","url":null,"abstract":"The chemical mutation is one of the important factors in the enrichment of the salt resistance capacity of the rhizobium isolates. Therefore, this study focused on 10 isolates of rhizobium collected from soil samples of Babile, Hararghe region, Ethiopia. All the collected isolates turned into a moderately yellow, yellow, and deep yellow color in yeast extract mannitol agar medium (YEMA) containing bromothymol blue after 48 h of incubation. It indicated that all the isolates were acid-producing Rhizobia. Moreover, based on colony morphology and diameter, 70% of the isolates displayed large mucoid colonies, and 30% of the isolates showed large watery colonies in YEMA media. Among the 10 isolates, 9(90%), 9(90%), 6(60%), 6(60%), 2(20%), and 1(10%), were grown at different salinity levels such as 2%, 4%, 6%, 8%, 9% and 10% of NaCl, correspondingly. The most salt-resistant wild isolate was HUCR 6 collected from Babile soil grown at 10% NaCl salinity level. A total of six mutants were considered after chemical mutagenesis based on their capacity to survive in the extreme salinity levels (11 to 14%). Mutant isolates such as HUCRM 4, HUCRM 5, and HUCRM 6 were the most tolerant Rhizobium that grew at the salinity level of 11 to 14% NaCl. The most sensitive mutant isolate was HUCRM 10 followed by the isolates HUCRM 8 and HUCRM 9 was the next sensitive mutant Rhizobia that grew only at 11% of NaCl concentration. Compared to the Rhizobium wild isolates, the mutant isolates were observed to be more tolerant to a medium containing higher concentration of NaCl, as high as 11% to 14%. Besides, 80% of the mutant isolates demonstrated effective nodulation with the common beans. The mutant isolate (HUCRM 4) showed better performance in relation to root nodule performance of Rhizobium species and increased the plant biomass production. In this study, mutant isolates HUCRM 6, which is tolerated to 14% NaCl, and HUCRM 4, HUCRM 5, and HUCRM 6 isolates tolerated at 12% salinity level. Finally, based on their symbiotic efficiency and tolerance to extreme salt levels, these mutant isolates (HUCRM 4, HUCRM 5 and HUCRM 6) were encouraged to be used for the development of Rhizobium inoculants of common beans grown under extreme saline conditions. J. Bio-Sci. 30(2): 13-22, 2022","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135817038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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