Free Ph3Sn(CH2)nOH (n = 3, 4, 6, 8 and 11) and immobilized organotin(IV) compounds, SBA- 15~Cl|Ph3Sn(CH2)nOH, were prepared and tested against different tumor cell lines. Both compounds and nanomaterials revealed strong cytotoxic potential. SBA-15~Cl|Ph3Sn(CH2)3OH as well as free compound induce caspase triggered apoptosis in human ovarian A2780 cells. Ph3Sn(CH2)6OH and corresponding nanomaterial induced apoptosis in mouse melanoma B16 cells. Survived clones of B16 cells demonstrated phenotypic changes, they differentiate toward melanocytes.
{"title":"MESOPOROUS SILICA AS A VEHICLE FOR ORGANOTIN(IV) COMPOUNDS","authors":"G. Kaluđerović","doi":"10.46793/iccbi21.060k","DOIUrl":"https://doi.org/10.46793/iccbi21.060k","url":null,"abstract":"Free Ph3Sn(CH2)nOH (n = 3, 4, 6, 8 and 11) and immobilized organotin(IV) compounds, SBA- 15~Cl|Ph3Sn(CH2)nOH, were prepared and tested against different tumor cell lines. Both compounds and nanomaterials revealed strong cytotoxic potential. SBA-15~Cl|Ph3Sn(CH2)3OH as well as free compound induce caspase triggered apoptosis in human ovarian A2780 cells. Ph3Sn(CH2)6OH and corresponding nanomaterial induced apoptosis in mouse melanoma B16 cells. Survived clones of B16 cells demonstrated phenotypic changes, they differentiate toward melanocytes.","PeriodicalId":9171,"journal":{"name":"Book of Proceedings: 1st International Conference on Chemo and BioInformatics,","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77144056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aleksandra M. Milošković, Nataša M. Kojadinović, Milena D. Radenković, Simona R. Đuretanović, T. Veličković, Marijana Nikolić, V. Simić
The utilization of fish bioindicators has become of crucial importance for ecosystem contamination assessment. Bleak (Alburnus alburnus) is a widely distributed epipelagic fish species characterized as very active with fast metabolism which can lead to a high accumulation of pollutants in tissues. Bleak is easy to sample, identify in the field by morphological characteristics, and easy to work within the laboratory. This study deals with the bleak sensitivity in detecting the pressure of the potentially toxic elements (PTEs) in the large lowland Great Morava River. The Al, As, Cd, Co, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Se, Sn, and Zn were estimated in whole body composite (wbc) of bleak. The study was conducted at Great Morava River, near the city of Paraćin in Serbia. The highest concentrations of essential elements Zn and Fe were detected. The most toxic elements As and Hg were below detection limits. The metal pollution index (MPI) was 0.39. In large lowland rivers, point sources of pollution such as municipal wastewaters of smaller settlements usually have only local impact due to high dilution by the main watercourse. Since bleak is a site-specific species, results in this study indicated that this stretch of the Great Morava River is slightly affected by PTEs. None of the elements above the maximum permitted concentrations (MPC) in fish meat for use in the human diet, prescribed by the Official Gazette and European Commission Regulation, were not recorded.
{"title":"POTENTIALLY TOXIC ELEMENTS IN LOWLAND GREAT MORAVA RIVER – BIOINDICATION WITH BLEAK (ALBURNUS ALBURNUS)","authors":"Aleksandra M. Milošković, Nataša M. Kojadinović, Milena D. Radenković, Simona R. Đuretanović, T. Veličković, Marijana Nikolić, V. Simić","doi":"10.46793/iccbi21.097m","DOIUrl":"https://doi.org/10.46793/iccbi21.097m","url":null,"abstract":"The utilization of fish bioindicators has become of crucial importance for ecosystem contamination assessment. Bleak (Alburnus alburnus) is a widely distributed epipelagic fish species characterized as very active with fast metabolism which can lead to a high accumulation of pollutants in tissues. Bleak is easy to sample, identify in the field by morphological characteristics, and easy to work within the laboratory. This study deals with the bleak sensitivity in detecting the pressure of the potentially toxic elements (PTEs) in the large lowland Great Morava River. The Al, As, Cd, Co, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Se, Sn, and Zn were estimated in whole body composite (wbc) of bleak. The study was conducted at Great Morava River, near the city of Paraćin in Serbia. The highest concentrations of essential elements Zn and Fe were detected. The most toxic elements As and Hg were below detection limits. The metal pollution index (MPI) was 0.39. In large lowland rivers, point sources of pollution such as municipal wastewaters of smaller settlements usually have only local impact due to high dilution by the main watercourse. Since bleak is a site-specific species, results in this study indicated that this stretch of the Great Morava River is slightly affected by PTEs. None of the elements above the maximum permitted concentrations (MPC) in fish meat for use in the human diet, prescribed by the Official Gazette and European Commission Regulation, were not recorded.","PeriodicalId":9171,"journal":{"name":"Book of Proceedings: 1st International Conference on Chemo and BioInformatics,","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88801334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The total quantity of metals (Mg, Ca, Ni, Fe, Mn, Zn, Cu) in soil samples and in sixteen different extracts from plant parts of Peucedanum oreoselinum (L.) Moench as well as the content of total phenols and flavonoids in plant extracts was determined. The contents of metals were determined by the atomic absorption spectrometer. Based on the average values of the metal concentration in the soil, they could be arranged in the following sequence: Fe > Ca > Mg > Mn > Zn > Cu > Ni. Soil concentrations of all tested metals were lower than the maximum allowed concentration. The results demonstrated that the analyzed plant extracts contained higher quantities of Ni and Ca. Although the studied species accumulate analyzed metals in different quantities, they are not hyperaccumulators of these metals. Total phenols were determined using Folin-Ciocalteu reagent and their amounts ranged from 1.94 to P. oreoselinum, hyperaccumulation, phenols, flavonoids32.38 mg GA/g. The amounts of flavonoids in plant extracts were in range from 0.69 to 25.83 mg RU/g. We examined the correlation of metals and the phenolic compounds content in the extracts. According to our results the use P. oreoselinum for tea preparation is safe to a great extent for people, because in spite of the determined metal absorption by plant organs, the tea does not contain dangerous quantity of heavy metals. Also, it is suitable for the preparation of teas and herbal extracts due to minimal content of toxic metal (Ni), phenols and flavonoids.
土壤样品和16种不同提取物中金属(Mg、Ca、Ni、Fe、Mn、Zn、Cu)的总量测定了植物提取物中总酚和总黄酮的含量。用原子吸收光谱仪测定了金属的含量。根据土壤中金属浓度平均值排列顺序为:Fe > Ca > Mg > Mn > Zn > Cu > Ni。所有被测金属的土壤浓度均低于最大允许浓度。结果表明，所分析的植物提取物中含有较高的Ni和Ca含量。虽然所研究的物种积累所分析的金属的量不同，但它们不是这些金属的超积累者。用Folin-Ciocalteu试剂测定总酚含量为1.94 ~ P. oreoselinum、超积累、酚类、黄酮类32.38 mg GA/g。植物提取物中总黄酮含量为0.69 ~ 25.83 mg RU/g。考察了金属与提取物中酚类化合物含量的相关性。根据我们的研究结果，在很大程度上，用奥利索林制茶对人是安全的，因为尽管植物器官对金属的吸收是确定的，但茶中重金属的含量并不高。此外，由于有毒金属(Ni)、酚类和类黄酮含量极低，因此适用于制备茶和草药提取物。
{"title":"DETERMINATION OF HEAVY METALS AND SECONDARY METABOLITES OF „PEUCEDANUM OREOSELINUM“ (APIACEAE)","authors":"M. Pavlović, Z. Simić, G. Đelić","doi":"10.46793/iccbi21.206p","DOIUrl":"https://doi.org/10.46793/iccbi21.206p","url":null,"abstract":"The total quantity of metals (Mg, Ca, Ni, Fe, Mn, Zn, Cu) in soil samples and in sixteen different extracts from plant parts of Peucedanum oreoselinum (L.) Moench as well as the content of total phenols and flavonoids in plant extracts was determined. The contents of metals were determined by the atomic absorption spectrometer. Based on the average values of the metal concentration in the soil, they could be arranged in the following sequence: Fe > Ca > Mg > Mn > Zn > Cu > Ni. Soil concentrations of all tested metals were lower than the maximum allowed concentration. The results demonstrated that the analyzed plant extracts contained higher quantities of Ni and Ca. Although the studied species accumulate analyzed metals in different quantities, they are not hyperaccumulators of these metals. Total phenols were determined using Folin-Ciocalteu reagent and their amounts ranged from 1.94 to P. oreoselinum, hyperaccumulation, phenols, flavonoids32.38 mg GA/g. The amounts of flavonoids in plant extracts were in range from 0.69 to 25.83 mg RU/g. We examined the correlation of metals and the phenolic compounds content in the extracts. According to our results the use P. oreoselinum for tea preparation is safe to a great extent for people, because in spite of the determined metal absorption by plant organs, the tea does not contain dangerous quantity of heavy metals. Also, it is suitable for the preparation of teas and herbal extracts due to minimal content of toxic metal (Ni), phenols and flavonoids.","PeriodicalId":9171,"journal":{"name":"Book of Proceedings: 1st International Conference on Chemo and BioInformatics,","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84112873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Simona R. Đuretanović, T. Veličković, Aleksandra M. Milošković, Milena D. Radenković, Marijana Nikolić, I. Maguire, V. Simić
The noble crayfish is one of the three autochthonous species that inhabit the freshwater ecosystems of Serbia, along with stone and Danube crayfish. The noble crayfish has a complex historical and genetic status shaped by geological events, habitat loss, pollution, translocations, and reintroductions of both autochthonous and allochthonous crayfish species. That led to the disruption of the species genetic structure, mixing, and loss of populations across Europe. According to recent data, its populations in the freshwater ecosystems of Serbia are significantly reduced, so it has the status of a "strictly protected species". The genetic structure of the species must be known for endangered species conservation. Unfortunately, there is lack of such data for the territory of Serbia, which due to its position on the Balkan Peninsula, was an important refuge during the glaciation period. In this paper, the genetic structure of seven crayfish populations in freshwater ecosystems of Serbia was examined. Analyzes were performed on the COI and 16S rRNA genes of mitochondrial DNA. The study results showed a significant diversity of COI and 16S rRNA haplotypes compared to already described haplotypes. Three haplotypes were detected, of which Hap26 is the most common and was detected in five studied populations. Haplotypes Hap47 and Hap49 were detected in one and two populations, respectively. The results obtained in this study, together with previously published morphometric data, represent a good starting point for further genetic and population research, which are the basis for the proposal of conservation measures.
{"title":"PRELIMINARY RESULTS REGARDING PHYLOGENY OF THE NOBLE CRAYFISH (DECAPODA, ASTACIDAE, „ASTACUS ASTACUS“) IN SERBIA","authors":"Simona R. Đuretanović, T. Veličković, Aleksandra M. Milošković, Milena D. Radenković, Marijana Nikolić, I. Maguire, V. Simić","doi":"10.46793/iccbi21.222dj","DOIUrl":"https://doi.org/10.46793/iccbi21.222dj","url":null,"abstract":"The noble crayfish is one of the three autochthonous species that inhabit the freshwater ecosystems of Serbia, along with stone and Danube crayfish. The noble crayfish has a complex historical and genetic status shaped by geological events, habitat loss, pollution, translocations, and reintroductions of both autochthonous and allochthonous crayfish species. That led to the disruption of the species genetic structure, mixing, and loss of populations across Europe. According to recent data, its populations in the freshwater ecosystems of Serbia are significantly reduced, so it has the status of a \"strictly protected species\". The genetic structure of the species must be known for endangered species conservation. Unfortunately, there is lack of such data for the territory of Serbia, which due to its position on the Balkan Peninsula, was an important refuge during the glaciation period. In this paper, the genetic structure of seven crayfish populations in freshwater ecosystems of Serbia was examined. Analyzes were performed on the COI and 16S rRNA genes of mitochondrial DNA. The study results showed a significant diversity of COI and 16S rRNA haplotypes compared to already described haplotypes. Three haplotypes were detected, of which Hap26 is the most common and was detected in five studied populations. Haplotypes Hap47 and Hap49 were detected in one and two populations, respectively. The results obtained in this study, together with previously published morphometric data, represent a good starting point for further genetic and population research, which are the basis for the proposal of conservation measures.","PeriodicalId":9171,"journal":{"name":"Book of Proceedings: 1st International Conference on Chemo and BioInformatics,","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91186718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tina P. Andrejević, Darko P Ašanin, Nada D. Savić, N. Stevanović, M. Djuran, Biljana Đ. Glišić
In recent decades, a special attention has been devoted to gold(III) complexes as potential antitumor agents due to their structural similarity to platinum(II) complexes. One of the possible mechanisms of the mode of antitumor activity of gold(III) complexes could include their interaction with DNA. However, the effectiveness of the therapeutic agents also depends on the degree of its binding to proteins present in the blood plasma, because, in this way, it is transported to the cell. Considering this, we investigated the interactions of three dinuclear gold(III) complexes of the general formula [{AuCl3}2(μ– L)], L = 4,4’-bipy (4,4’-bipyridine, Au1), bpe (1,2-bis(4-pyridyl)ethane, Au2) and dpe (1,2-bis(4- pyridyl)ethene, Au3) with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA). The main aim of the study was to evaluate the binding affinities of gold(III) complexes Au1–3 towards these biomolecules for possible insights on their mode of biological activity. The values of binding constants (KA) of Au1–3 to ct-DNA are higher than those for BSA, indicating greater affinity of the complexes towards this nucleic acid. The partition coefficient (logP) value for Au1 is higher compared to the corresponding values for the other two complexes, what is in accordance with a higher cellular uptake efficiency of this complex.
{"title":"DNA/BSA BINDING STUDY OF DINUCLEAR GOLD(III) COMPLEXES WITH AROMATIC NITROGEN-CONTAINING HETEROCYCLES AS BRIDGING LIGANDS","authors":"Tina P. Andrejević, Darko P Ašanin, Nada D. Savić, N. Stevanović, M. Djuran, Biljana Đ. Glišić","doi":"10.46793/iccbi21.312a","DOIUrl":"https://doi.org/10.46793/iccbi21.312a","url":null,"abstract":"In recent decades, a special attention has been devoted to gold(III) complexes as potential antitumor agents due to their structural similarity to platinum(II) complexes. One of the possible mechanisms of the mode of antitumor activity of gold(III) complexes could include their interaction with DNA. However, the effectiveness of the therapeutic agents also depends on the degree of its binding to proteins present in the blood plasma, because, in this way, it is transported to the cell. Considering this, we investigated the interactions of three dinuclear gold(III) complexes of the general formula [{AuCl3}2(μ– L)], L = 4,4’-bipy (4,4’-bipyridine, Au1), bpe (1,2-bis(4-pyridyl)ethane, Au2) and dpe (1,2-bis(4- pyridyl)ethene, Au3) with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA). The main aim of the study was to evaluate the binding affinities of gold(III) complexes Au1–3 towards these biomolecules for possible insights on their mode of biological activity. The values of binding constants (KA) of Au1–3 to ct-DNA are higher than those for BSA, indicating greater affinity of the complexes towards this nucleic acid. The partition coefficient (logP) value for Au1 is higher compared to the corresponding values for the other two complexes, what is in accordance with a higher cellular uptake efficiency of this complex.","PeriodicalId":9171,"journal":{"name":"Book of Proceedings: 1st International Conference on Chemo and BioInformatics,","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90509944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tanja Pajić, N. Todorović, Dunja Stefanovic, M. Rabasović, A. Krmpot, M. Živić
Third Harmonic Generation (THG) microscopy was employed as a method of choice for lipid droplet (LD) measurements and quantification of the effect of selenite on LDs. Nonlinear laser scanning microscopy (NLSM) employs ultra-short laser pulses for imaging. THG microscopy is the modality of NLSM. Strong THG signals can only be observed from regions with non- uniformities with respect to their refractive index. Such regions in biological samples are lipid-water interfaces, and by far the brightest features in cells are LDs. For that reason, THG microscopy is the appropriate method for imaging of LDs from live unfixed cells, without the need for additional labeling. The biological effects of spore- to- end- of- exponential- phase duration (27 – 30 h) of exposure to 1 mM selenite were monitored in vivo on the cells of filamentous fungi in liquid culture. We measured the lipid droplet density and size distribution in a model fungi Phycomyces blakesleeanus. The in-house built microscope frame complemented with Yb KGW laser (1040 nm, 200 fs pulses) was used, while detection was enabled in the transmission arm by PMT through the Hoya glass UV filter (peak at 340 nm). From THG images of control and Se+4–treated hyphae, LD size and number were measured, showing that LD density was increased by more than 60% in Se+4–treated hyphae, compared to control. The average LD size distribution seemed slightly changed by Se+4 -treatment. The obtained results suggest that 1 mM selenite treatment probably induces cellular stress response in filamentous fungi.
{"title":"THE EFFECTS OF SELENITE ON FILAMENTOUS FUNGI LIPID DROPLETS MONITORED „IN VIVO“ LABEL FREE USING ADVANCED NONLINEAR MICROSCOPY TECHNIQUE 2021ICCBIKG (2021)","authors":"Tanja Pajić, N. Todorović, Dunja Stefanovic, M. Rabasović, A. Krmpot, M. Živić","doi":"10.46793/iccbi21.300p","DOIUrl":"https://doi.org/10.46793/iccbi21.300p","url":null,"abstract":"Third Harmonic Generation (THG) microscopy was employed as a method of choice for lipid droplet (LD) measurements and quantification of the effect of selenite on LDs. Nonlinear laser scanning microscopy (NLSM) employs ultra-short laser pulses for imaging. THG microscopy is the modality of NLSM. Strong THG signals can only be observed from regions with non- uniformities with respect to their refractive index. Such regions in biological samples are lipid-water interfaces, and by far the brightest features in cells are LDs. For that reason, THG microscopy is the appropriate method for imaging of LDs from live unfixed cells, without the need for additional labeling. The biological effects of spore- to- end- of- exponential- phase duration (27 – 30 h) of exposure to 1 mM selenite were monitored in vivo on the cells of filamentous fungi in liquid culture. We measured the lipid droplet density and size distribution in a model fungi Phycomyces blakesleeanus. The in-house built microscope frame complemented with Yb KGW laser (1040 nm, 200 fs pulses) was used, while detection was enabled in the transmission arm by PMT through the Hoya glass UV filter (peak at 340 nm). From THG images of control and Se+4–treated hyphae, LD size and number were measured, showing that LD density was increased by more than 60% in Se+4–treated hyphae, compared to control. The average LD size distribution seemed slightly changed by Se+4 -treatment. The obtained results suggest that 1 mM selenite treatment probably induces cellular stress response in filamentous fungi.","PeriodicalId":9171,"journal":{"name":"Book of Proceedings: 1st International Conference on Chemo and BioInformatics,","volume":"69 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84275200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Filipović, T. Vasić, N. Radulović, D. Jevremović, I. Stanojević
Myrtus communis L., Myrtaceae, known as true myrtle, is a widely distributed evergreen shrub native to Mediterranean regions. This medicinal plant, used worldwide, possess a broad spectrum of phytochemical, pharmacological and therapeutic effects, mostly due to a large number of up to now detected, or isolated, essential oil components, considered the main biologically active ones. Multifarious secondary metabolite content, dependent on a geographic region, season of harvest and the length of distillation, depicts the variability of the essential oil composition obtained in general from its leaves, branches, fruits and flowers. Herein, we present the results of meticulous analyses of the essential oil (2.2%, w/w) constituents obtained from M. communis leaves, collected during the summer period from the coastal regions of peninsula Luštica, Montenegro. Detailed GC-MS analyses enabled the identification of 66 constituents, among which 1,8-cineole (28.4%), linalool (18.4%), α-pinene (16.6%), geranyl acetate (6.6%), α-terpineol (6.3%) and linalyl acetate (4.2%) were the major ones. Together with the secondary metabolite profile, determination of the Montenegro true myrtle essential oil agricultural plant protection potential is also estimated, by assessing mycelia growth of Colletotrichum acutatum J.H. Simmonds C.A.2 isolates, causative of strawberry antrachnose, in vitro.
Myrtus communis L.，桃金娘科，被称为真桃金娘，是一种广泛分布的常绿灌木，原产于地中海地区。这种药用植物在世界范围内使用，具有广泛的植物化学，药理和治疗作用，主要是由于大量迄今为止检测到或分离到的精油成分，被认为是主要的生物活性成分。不同的次生代谢物含量，取决于地理区域、收获季节和蒸馏时间，描绘了从其叶子、树枝、果实和花朵中获得的精油成分的变化。在此，我们展示了对夏季期间从黑山Luštica半岛沿海地区收集的M. communis叶子中获得的精油(2.2%，w/w)成分的细致分析结果。经GC-MS分析，共鉴定出66种主要成分，其中1,8-桉树油脑(28.4%)、芳樟醇(18.4%)、α-蒎烯(16.6%)、乙酸香叶醇(6.6%)、α-松油醇(6.3%)和乙酸芳樟醇(4.2%)为主要成分。通过对草莓炭疽病菌acutatum J.H. Simmonds C.A.2分离株的体外菌丝生长情况进行评价，并结合次生代谢物谱，对黑山真桃金桃精油的农业植物保护潜力进行了评价。
{"title":"DETAILED GC-MS ANALYSES OF THE „MYRTUS COMMUNIS“ L., ESSENTIAL OIL AND THE ANTIFUNGAL ACTIVITY ASSESSMENT","authors":"S. Filipović, T. Vasić, N. Radulović, D. Jevremović, I. Stanojević","doi":"10.46793/iccbi21.276f","DOIUrl":"https://doi.org/10.46793/iccbi21.276f","url":null,"abstract":"Myrtus communis L., Myrtaceae, known as true myrtle, is a widely distributed evergreen shrub native to Mediterranean regions. This medicinal plant, used worldwide, possess a broad spectrum of phytochemical, pharmacological and therapeutic effects, mostly due to a large number of up to now detected, or isolated, essential oil components, considered the main biologically active ones. Multifarious secondary metabolite content, dependent on a geographic region, season of harvest and the length of distillation, depicts the variability of the essential oil composition obtained in general from its leaves, branches, fruits and flowers. Herein, we present the results of meticulous analyses of the essential oil (2.2%, w/w) constituents obtained from M. communis leaves, collected during the summer period from the coastal regions of peninsula Luštica, Montenegro. Detailed GC-MS analyses enabled the identification of 66 constituents, among which 1,8-cineole (28.4%), linalool (18.4%), α-pinene (16.6%), geranyl acetate (6.6%), α-terpineol (6.3%) and linalyl acetate (4.2%) were the major ones. Together with the secondary metabolite profile, determination of the Montenegro true myrtle essential oil agricultural plant protection potential is also estimated, by assessing mycelia growth of Colletotrichum acutatum J.H. Simmonds C.A.2 isolates, causative of strawberry antrachnose, in vitro.","PeriodicalId":9171,"journal":{"name":"Book of Proceedings: 1st International Conference on Chemo and BioInformatics,","volume":"182 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81573336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tanja Pajić, N. Todorović, Dunja Stefanovic, M. Rabasović, A. Krmpot, M. Živić
Selenium salts have been known for long time to have a potential for both beneficial and harmful effects on living organisms. It is present in the environment, where it can be readily assimilated by plants and fungi, thus entering the food chain. We investigated the cell growth dynamics in the presence of selenite which is considered to have more toxic potential than selenate. The effects of selenite (1 mM) on the growth of fungi from the activated spores to the end of the exponential growth were measured on several hypha morphological parameters by microscopy in vivo. Phycomyces blaekesleneeanus was used as model filamentous fungus. The most striking effect of Se+4 treatment was inhibition of hypha growth, resulting in more than four times shorter hypha in Se+4 –treatment group than in the control (200 ± 50 µm, n = 50 vs 900 ± 100 µm, n = 40 respectively) at the end of exponential growth period under controlled conditions. The Se+4 effect was an inhibition and not a simple delay in growth, as hypha length did not change significantly from 27th to 30th hour of culture in Se+4-treatment group. Since the microscopy was performed on live cultured cells, undisturbed cytoplasmic streaming was observed, confirming that hyphae were alive at all time points measured. 30h old spore diameters were also significantly reduced by Se+4 treatment (p = 0.0365), while hypha diameters were not significantly altered.
硒盐长期以来一直被认为对生物体有潜在的有益和有害的影响。它存在于环境中，很容易被植物和真菌吸收，从而进入食物链。我们研究了亚硒酸盐存在时的细胞生长动力学，亚硒酸盐被认为比硒酸盐具有更大的毒性。在体内显微镜下观察亚硒酸盐(1 mM)对真菌从激活孢子到指数生长末期的生长情况的影响。以黑藻为模型丝状真菌。Se+4处理最显著的效果是抑制菌丝生长，在控制条件下，Se+4处理组的菌丝在指数生长期结束时(200±50µm, n = 50 vs 900±100µm, n = 40)比对照组短4倍以上。硒+4的作用是抑制而不是简单的延迟生长，因为在培养第27 ~ 30小时，硒+4处理组菌丝长度没有显著变化。由于显微镜是在活培养细胞上进行的，观察到未受干扰的细胞质流动，证实在所有测量的时间点菌丝都是活的。Se+4处理也显著降低了30h孢子直径(p = 0.0365)，菌丝直径无显著变化。
{"title":"THE INFLUENCE OF SELENITE ON FILLAMENTOUS FUNGI HYPHA MORPHOMETRY PARAMETERS","authors":"Tanja Pajić, N. Todorović, Dunja Stefanovic, M. Rabasović, A. Krmpot, M. Živić","doi":"10.46793/iccbi21.308p","DOIUrl":"https://doi.org/10.46793/iccbi21.308p","url":null,"abstract":"Selenium salts have been known for long time to have a potential for both beneficial and harmful effects on living organisms. It is present in the environment, where it can be readily assimilated by plants and fungi, thus entering the food chain. We investigated the cell growth dynamics in the presence of selenite which is considered to have more toxic potential than selenate. The effects of selenite (1 mM) on the growth of fungi from the activated spores to the end of the exponential growth were measured on several hypha morphological parameters by microscopy in vivo. Phycomyces blaekesleneeanus was used as model filamentous fungus. The most striking effect of Se+4 treatment was inhibition of hypha growth, resulting in more than four times shorter hypha in Se+4 –treatment group than in the control (200 ± 50 µm, n = 50 vs 900 ± 100 µm, n = 40 respectively) at the end of exponential growth period under controlled conditions. The Se+4 effect was an inhibition and not a simple delay in growth, as hypha length did not change significantly from 27th to 30th hour of culture in Se+4-treatment group. Since the microscopy was performed on live cultured cells, undisturbed cytoplasmic streaming was observed, confirming that hyphae were alive at all time points measured. 30h old spore diameters were also significantly reduced by Se+4 treatment (p = 0.0365), while hypha diameters were not significantly altered.","PeriodicalId":9171,"journal":{"name":"Book of Proceedings: 1st International Conference on Chemo and BioInformatics,","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86765680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"QSRR MODELING OF LIQUID CHROMATOGRAPHY SEPARATION OF ZIPRASIDONE COMPOUNDS","authors":"Marija Čarapić, K. Nikolić, D. Agbaba","doi":"10.46793/iccbi21.284c","DOIUrl":"https://doi.org/10.46793/iccbi21.284c","url":null,"abstract":"","PeriodicalId":9171,"journal":{"name":"Book of Proceedings: 1st International Conference on Chemo and BioInformatics,","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87854768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Srećković, Jelena S. Katanić Stanković, V. Mihailović
Lysimachia vulgaris L., also known as yellow loosestrife, is an herbaceous perennial flowering plant from the family Primulaceae. Traditionally, L. vulgaris has been commonly used in Chinese medicine to increase urination, reduce fever, and treat sexually transmitted ailments. This study aimed to investigate phenolic content, antioxidant activity, and antimicrobial potential of L. vulgaris aerial part (LVA) and root (LVR) methanol extracts. Based on the spectrophotometric determination, both extracts possessed a high level of phenolic compounds. The content of total phenolic compounds (130.14 mg GAE/g) and condensed tannins (120.58 mg GAE/g) was higher in LVR, while total flavonoid (206.93 mg QUE/g) and flavanol (17.02 RUE/g) contents were higher in LVA. DPPH and ABTS assays were used to estimate the antioxidant activity of the extracts, whereby LVR showed better antioxidant potential in both applied methods. The extracts inhibited the growth of most of the tested bacterial and fungal strains with minimal inhibitory concentration values (MICs) ranged between (˂ 0.3125 to 20 mg/mL), whereby the significantly higher antimicrobial activity of LVR was observed. Based on the obtained results, the significant antioxidant and antimicrobial activity of L. vulgaris root extract may be a basis for its further research and potential application in the pharmaceutical and food industry.
{"title":"PHENOLIC CONTENT AND POTENTIAL APPLICATION OF „LYSIMACHIA VULGARIS“ L. AERIAL PART AND ROOT EXTRACTS","authors":"N. Srećković, Jelena S. Katanić Stanković, V. Mihailović","doi":"10.46793/iccbi21.395s","DOIUrl":"https://doi.org/10.46793/iccbi21.395s","url":null,"abstract":"Lysimachia vulgaris L., also known as yellow loosestrife, is an herbaceous perennial flowering plant from the family Primulaceae. Traditionally, L. vulgaris has been commonly used in Chinese medicine to increase urination, reduce fever, and treat sexually transmitted ailments. This study aimed to investigate phenolic content, antioxidant activity, and antimicrobial potential of L. vulgaris aerial part (LVA) and root (LVR) methanol extracts. Based on the spectrophotometric determination, both extracts possessed a high level of phenolic compounds. The content of total phenolic compounds (130.14 mg GAE/g) and condensed tannins (120.58 mg GAE/g) was higher in LVR, while total flavonoid (206.93 mg QUE/g) and flavanol (17.02 RUE/g) contents were higher in LVA. DPPH and ABTS assays were used to estimate the antioxidant activity of the extracts, whereby LVR showed better antioxidant potential in both applied methods. The extracts inhibited the growth of most of the tested bacterial and fungal strains with minimal inhibitory concentration values (MICs) ranged between (˂ 0.3125 to 20 mg/mL), whereby the significantly higher antimicrobial activity of LVR was observed. Based on the obtained results, the significant antioxidant and antimicrobial activity of L. vulgaris root extract may be a basis for its further research and potential application in the pharmaceutical and food industry.","PeriodicalId":9171,"journal":{"name":"Book of Proceedings: 1st International Conference on Chemo and BioInformatics,","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86108045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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