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Cutinolytic esterases are induced by growth of the fungus Trichoderma harzianum on glyceryl monostearate in solid-state fermentation 哈兹木霉在单硬脂酸甘油酯固态发酵中生长,诱导其产生溶皮酯酶
4区 农林科学 Q2 MATERIALS SCIENCE, PAPER & WOOD Pub Date : 2023-10-27 DOI: 10.15376/biores.18.4.8515-8527
Victoria Conde-Ávila, Octavio Loera-Corral, Rubén Díaz, Carmen Sánchez
Cutinolytic esterase are enzymes utilized in a wide variety of industrial applications, and they are capable of degrading emerging environmental pollutants. Due to the application and importance of these enzymes, it is crucial to develop an efficient method for cutinase production using a cost-effective inductor and an efficient microbial production system. In this work, the growth and cutinolytic esterase production of Trichoderma harzianum were evaluated in glucose-yeast extract media containing different glyceryl monostearate (GMS) concentrations (1, 3, and 5 g/L). It was used as inducer in solid-state fermentation. A medium lacking GMS was used as control. Biomass production and enzyme productivity were higher in inducer-added (1 g/L) medium than in the control medium. T. harzianum produced constitutive and inducible cutinolytic esterase, in which production was enhanced by GMS. In GMS-added cultures, two bands with cutinolytic esterase activity (60 and 150 kDa approximately) were observed by zymography, which were not observed in control culture. GMS represents a promising inducer for cutinolytic esterase production by fungi. This research represents the first approach for the study of cutinolytic esterase production using a synthetic molecule as an inducer.
皮溶酯酶是广泛应用于各种工业应用的酶,它们能够降解新出现的环境污染物。由于这些酶的应用和重要性,开发一种高效的方法来生产角质酶是至关重要的,该方法使用成本低廉的诱导剂和高效的微生物生产系统。在这项工作中,研究了哈茨木霉在葡萄糖-酵母提取物培养基中含有不同浓度的单硬脂酸甘油(GMS)(1、3和5 g/L)的生长和溶皮酯酶的产生。在固态发酵中用作诱导剂。用不含GMS的培养基作为对照。在添加诱导剂(1 g/L)的培养基中,生物量和酶产率均高于对照培养基。哈氏霉产生组成型和诱导型溶角质酯酶,GMS增强了其产量。在添加gms的培养物中,酶谱分析发现了两条具有溶皮酯酶活性的条带(约为60和150 kDa),而在对照培养物中没有观察到。GMS是一种很有前途的真菌促角质分解酯酶诱导剂。本研究首次采用人工合成分子作为诱导剂来研究溶角质酯酶的生产。
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引用次数: 0
Characterization of the properties of Buluh Madu (Gigantochloa albociliata) Buluh Madu (Gigantochloa albociliata)的特性研究
4区 农林科学 Q2 MATERIALS SCIENCE, PAPER & WOOD Pub Date : 2023-10-27 DOI: 10.15376/biores.18.4.8503-8514
Siti Asiyah Kasdi, Seng Hua Lee, Paridah Md. Tahir, Syeed Saifulazry Osman al-Edrus, Sabiha Salim, Muhammad Aizat Abd Ghani, Balkis Fatomer A. Bakar, Wei Chen Lum, Jun Zhang
Thirteen bamboo species are reported to be in commercial use in Malaysia. However, Buluh madu (Gigantochloa albociliata) did not make to the list. As a species, G. albociliata is cultivated for its delicious bamboo shoot and is demonstrated to possess great potential to produce commercialised products such as laminated bamboo panel. Unlike common bamboo, which has hollow cylindrical culms, G. albociliata has thick culms at the base, with smaller hollow cavities at the top portion. Therefore, it can be easily converted into high-thickness strips, thus improving the processing efficiency of laminated bamboo. To validate this theory, the anatomical, chemical, physical, and mechanical properties of G. albociliata were evaluated. The round bamboo and strips from the top and bottom sections of the bamboo stem were tested. It was found that G. albociliata has a vascular bundle type similar to that of the Gigantochloa genus bamboo. The fibre in G. albociliata is long and strong. The top section of bamboo has longer fibres, a higher density, and a higher specific gravity than the bottom section. As a result, bamboo from the top section has greater bending strength than bamboo from the bottom section. The G. albociliata species was discovered to have high mechanical strength, dimensional stability, and good wettability, making it an ideal material for laminated products.
据报道,在马来西亚有13种竹子被用于商业用途。然而,Buluh madu (Gigantochloa albociliata)没有上榜。作为一个物种,G. albociliata因其美味的竹笋而被种植,并被证明具有生产竹板等商业化产品的巨大潜力。普通竹子的茎是空心的圆柱形,与之不同的是,白竹的根部茎厚,顶部有较小的空心。因此,它可以很容易地转换成高厚度的条,从而提高竹片的加工效率。为了验证这一理论,我们对白桦的解剖、化学、物理和力学性能进行了评估。对圆竹和竹茎上、下段的竹条进行了试验。结果表明,竹的维管束类型与竹的维管束类型相似。白桦的纤维长而结实。竹子的顶部比底部有更长的纤维,更高的密度和更高的比重。因此,顶部的竹子比底部的竹子具有更大的抗弯强度。该材料具有较高的机械强度、尺寸稳定性和良好的润湿性,是层压制品的理想材料。
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引用次数: 0
Reducing particulates and gaseous emissions through fuel switching from coal to wood pellets at power plants in South Korea during 2005 to 2022 2005年至2022年期间,通过在韩国发电厂从煤炭转向木质颗粒燃料,减少颗粒物和气体排放
4区 农林科学 Q2 MATERIALS SCIENCE, PAPER & WOOD Pub Date : 2023-10-26 DOI: 10.15376/biores.18.4.8458-8472
Seung-Rok Lee, Sae Byul Kang, Gyu-Seong Han
This study analyzed the particulates and gaseous emissions from 2005 to 2022 for power plants in South Korea (Utility scale: 125 MW (B-1) and 200 MW (B-2), respectively), which recently successfully converted from coal to wood pellets. The analysis showed that (1) NOx reduction was 78.9 to 90.0% (with outlet denitrification facility), (2) SOx reduction was 95.0 to 99.6% (without desulfurization facility condition), and (3) total suspended particles (TSP) reduction was 70.3 to 87.2% (with improved filtration and dust collection facility). This research confirmed the capabilities of wood pellets as a baseload power source and demonstrated their superior NOx reduction compared to coal. In the case of SOx, the desulfurization facility was discontinued at the stage of the fuel switch, so the value was affected by exogenous variable factors other than fuel. The TSP appears to be a combination of the ‘fine dust’ contained in the wood pellets and the performance of the filtration dust collector. The results suggest that fuel switching to wood pellets is a viable alternative to fossil fuels as an appropriate climate technology.
该研究分析了最近成功将煤炭转化为木屑颗粒的韩国发电厂(公用事业规模:125兆瓦(B-1)和200兆瓦(B-2)) 2005年至2022年的颗粒物和气体排放情况。分析表明:(1)有出口脱硝装置时,NOx的降低率为78.9 ~ 90.0%;(2)无脱硫装置时,SOx的降低率为95.0 ~ 99.6%;(3)有改进过滤和集尘装置时,总悬浮粒子(TSP)的降低率为70.3 ~ 87.2%。这项研究证实了木屑颗粒作为基本负荷电源的能力,并证明了与煤炭相比,它们具有更好的氮氧化物还原能力。以SOx为例,由于在燃料切换阶段停止了脱硫设施,因此该值受到燃料以外的外生变量因素的影响。TSP似乎是木屑颗粒中含有的“细粉尘”和过滤除尘器性能的结合。结果表明,燃料转换为木屑颗粒是化石燃料的可行替代品,是一种适当的气候技术。
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引用次数: 0
Carbon emissions trading potential of Turkiye's forest 土耳其森林的碳排放交易潜力
4区 农林科学 Q2 MATERIALS SCIENCE, PAPER & WOOD Pub Date : 2023-10-26 DOI: 10.15376/biores.18.4.8409-8431
Nilay Tulukcu Yıldızbaş, Hülya Kılıç Hernández, Hülya Yıldırım, Yusuf Güneş
The current study emphasizes the inherent shortcomings of laws and policy approaches that are based on the premise that by increasing wood production, much more emission credits can be achieved by using wood in alternative uses. The article aims to exploit the financing of emission reductions, discuss how carbon sinks held in forest resources can be activated, traded, and financed, and explain how Turkiye’s forest carbon potential can be exploited. To make a comparative analysis of the situation of Turkiye at global level, Russian’s potential for carbon sequestration and its trade have been dealt with as a comparison by following quantitative research methodology. In this research, the calculation method has been used to determine the number of houses that are likely to be built in rural areas using wood materials, e.g., the construction of 100,000 houses with a construction area of 100 m2 per year. Consequently, the forest carbon generated by alternative scenarios contributes positively to the emission balance sheet, as well as climate change mitigation through carbon emission trade despite all legal and technical constraints. Although both countries have similar shortcomings of obtaining carbon credits and its trade, of course Russia has a promising situation in comparison with Turkiye with respect to the amount of carbon sequestered and the likelihood of its trade potential at global level.
目前的研究强调了法律和政策办法的固有缺点,这些办法的前提是,通过增加木材生产,可以通过将木材用于其他用途而获得更多的排放信用额。本文旨在利用减排融资,讨论如何激活、交易和融资森林资源中的碳汇,并解释如何利用土耳其的森林碳潜力。为了在全球范围内对土耳其的情况进行比较分析,本文采用定量研究方法对俄罗斯的碳封存潜力及其贸易进行了比较。在本研究中,使用计算方法来确定农村地区可能使用木质材料建造的房屋数量,例如每年建造10万套建筑面积为100平方米的房屋。因此,尽管存在各种法律和技术限制,但替代情景产生的森林碳对排放资产负债表以及通过碳排放贸易缓解气候变化作出了积极贡献。虽然这两个国家在获得碳信用额及其贸易方面都有类似的缺点,但与土耳其相比,俄罗斯在碳封存量和全球贸易潜力方面当然有希望。
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引用次数: 0
TSW-YOLO-v8n: Optimization of detection algorithms for surface defects on sawn timber TSW-YOLO-v8n:锯材表面缺陷检测算法优化
4区 农林科学 Q2 MATERIALS SCIENCE, PAPER & WOOD Pub Date : 2023-10-26 DOI: 10.15376/biores.18.4.8444-8457
Mingtao Wang, Mingxi Li, Wenyan Cui, Xiaoyang Xiang, Huaqiong Duo
The goal of this work was to better meet the demand for rapid detection of surface defects in sawn timber in forestry production. This paper introduces a two-way feature fusion network based on the YOLO-v8 algorithm and proposes a feature fusion network model that combines the attention mechanism and loss function optimization. In this way it increases the tiny target detection head in order to more effectively detect small defective targets in the wood, thus realizing the model’s high-efficiency and low-consumption functional design. The results show that the improved TSW-YOLO-v8n model realized the identification of eight kinds of defects in sawn timber with a high efficiency of 91.10% mAP50 and an average detection 6 ms, which is 5.1% higher than the original model’s mAP50 and 1 ms shorter than the original model’s average detection time. The comparison of the original model and its mainstream algorithms shows that the model of this paper had better performance and better detection capability. Thus, the improved model achieved better overall performance and stronger detection ability, which provides a new idea for the development of detection technology in the forestry industry.
这项工作的目的是为了更好地满足林业生产中对锯材表面缺陷快速检测的需求。本文介绍了一种基于YOLO-v8算法的双向特征融合网络,提出了一种将注意力机制与损失函数优化相结合的特征融合网络模型。这样增加了微小目标检测头,以便更有效地检测木材中的微小缺陷目标,从而实现了模型的高效低耗功能设计。结果表明,改进的TSW-YOLO-v8n模型实现了对8种锯材缺陷的识别,mAP50的识别率高达91.10%,平均检测时间为6 ms,比原模型的mAP50提高了5.1%,比原模型的平均检测时间缩短了1 ms。将原始模型与主流算法进行比较,表明本文模型具有更好的性能和更好的检测能力。改进后的模型整体性能更好,检测能力更强,为林业行业检测技术的发展提供了新的思路。
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引用次数: 0
Hydrophobic microcrystalline cellulose/polyethyleneimine composite aerogel for effective sound absorption 疏水微晶纤维素/聚乙烯亚胺复合气凝胶有效吸声
4区 农林科学 Q2 MATERIALS SCIENCE, PAPER & WOOD Pub Date : 2023-10-26 DOI: 10.15376/biores.18.4.8432-8443
Xin Jia, Guijiang Tang, Jinming Gao, Yangmiao Liao, Yu Zhang, Xueliang Jiang, Huan Yang, Dan Wu, Feng You, Peng Yu, Chu Yao
A hydrophobic and ultralight cellulose aerogel (CA) was reinforced by polyethyleneimine (PEI) and functionalized by methyltrimethoxysilane (MTMS). Adding PEI improved the mechanical strength and the elastic resilience of the resulting material due to the flexibility enhancement of the cellulose chains, which prevented the collapse of the pore structure and contributed to the uniform pore size distribution. The hydrophobic property of the aerogels with the functionalization of MTMS was improved, which can prevent the pore structure from collapsing due to the absorption of water. The maximum compression modulus of aerogel reached 1.1 MPa at the strain of 80%, and its hydrophobic water contact angle was up to 112°. The hydrophobic composite aerogels exhibited ultrahigh efficiency in sound absorption across a wide frequency range from 500 to 6300 Hz, and their average absorption coefficient was greater than 0.74. The light weight, high porosity, and environmentally friendly aerogels presented in this work are promising for efficient sound absorption. They have potential applications in noise pollution treatment.
以聚乙烯亚胺(PEI)为增强剂,甲基三甲氧基硅烷(MTMS)为功能化剂,制备了一种疏水超轻纤维素气凝胶(CA)。由于纤维素链的柔韧性增强,PEI的加入提高了材料的机械强度和弹性回弹,防止了孔隙结构的崩溃,使孔隙尺寸分布均匀。MTMS功能化后的气凝胶的疏水性得到了改善,可以防止孔隙结构因吸水而崩溃。在80%的应变下,气凝胶的最大压缩模量达到1.1 MPa,疏水接触角达到112°。在500 ~ 6300 Hz的宽频率范围内,复合气凝胶具有超高的吸声效率,平均吸声系数大于0.74。本研究中提出的轻质、高孔隙度、环保的气凝胶有望实现高效吸声。它们在噪声污染治理中具有潜在的应用前景。
{"title":"Hydrophobic microcrystalline cellulose/polyethyleneimine composite aerogel for effective sound absorption","authors":"Xin Jia, Guijiang Tang, Jinming Gao, Yangmiao Liao, Yu Zhang, Xueliang Jiang, Huan Yang, Dan Wu, Feng You, Peng Yu, Chu Yao","doi":"10.15376/biores.18.4.8432-8443","DOIUrl":"https://doi.org/10.15376/biores.18.4.8432-8443","url":null,"abstract":"A hydrophobic and ultralight cellulose aerogel (CA) was reinforced by polyethyleneimine (PEI) and functionalized by methyltrimethoxysilane (MTMS). Adding PEI improved the mechanical strength and the elastic resilience of the resulting material due to the flexibility enhancement of the cellulose chains, which prevented the collapse of the pore structure and contributed to the uniform pore size distribution. The hydrophobic property of the aerogels with the functionalization of MTMS was improved, which can prevent the pore structure from collapsing due to the absorption of water. The maximum compression modulus of aerogel reached 1.1 MPa at the strain of 80%, and its hydrophobic water contact angle was up to 112°. The hydrophobic composite aerogels exhibited ultrahigh efficiency in sound absorption across a wide frequency range from 500 to 6300 Hz, and their average absorption coefficient was greater than 0.74. The light weight, high porosity, and environmentally friendly aerogels presented in this work are promising for efficient sound absorption. They have potential applications in noise pollution treatment.","PeriodicalId":9172,"journal":{"name":"Bioresources","volume":"37 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134906100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Simulation and experimental analysis of Camellia oleifera fruit shedding based on finite element explicit dynamics 基于有限元显式动力学的油茶果实脱落模拟与实验分析
4区 农林科学 Q2 MATERIALS SCIENCE, PAPER & WOOD Pub Date : 2023-10-22 DOI: 10.15376/biores.18.4.8394-8408
Fanyu Wang, Jianbo Zhou, Zhengkun Miao, Yanhe Liu, Haiyun Feng, Yongjie Lei, Tianyu Wang, Chenkun Xiong
As an important oil crop in China and the world, the harvesting problem of Camellia oleifera has attracted much attention. Research is needed on mechanical characteristics of harvesting equipment. Explicit dynamics was used to establish a finite element model under a simulated load response to the branch-pedicel-fruit system of C. oleifera to predict the fracture process at the pedicel junction. The separation mechanism of C. oleifera fruit was determined by measuring the constitutive parameters of fruit branches and pedicels and conducting separation experiments and explicit dynamics simulations on different hanging fruits. The maximum stress at the fruit pedicel was 1.14 MPa, and the goodness of fit between the simulation and experiment was approximately 89.5%, indicating that the branch-pedicel-fruit finite element model could accurately reflect the fruit shedding process and that the pedicel diameter was correlated positively with the separation force. This study provides technical parameters for the optimized design of existing C. oleifera harvesting equipment.
油茶作为中国乃至世界重要的油料作物,其采收问题备受关注。需要对采收设备的机械特性进行研究。采用显式动力学方法,建立了油油树枝-花梗-果实系统模拟载荷响应的有限元模型,预测了花梗连接处的断裂过程。通过测定果枝和花梗的本构参数,并对不同挂果进行分离实验和显式动力学模拟,确定了油桐果实的分离机理。果蒂处的最大应力为1.14 MPa,仿真与实验拟合度约为89.5%,表明所建立的枝-花梗-果实有限元模型能较准确地反映果实脱落过程,且果蒂直径与分离力呈正相关。本研究为现有油桐采收设备的优化设计提供了技术参数。
{"title":"Simulation and experimental analysis of Camellia oleifera fruit shedding based on finite element explicit dynamics","authors":"Fanyu Wang, Jianbo Zhou, Zhengkun Miao, Yanhe Liu, Haiyun Feng, Yongjie Lei, Tianyu Wang, Chenkun Xiong","doi":"10.15376/biores.18.4.8394-8408","DOIUrl":"https://doi.org/10.15376/biores.18.4.8394-8408","url":null,"abstract":"As an important oil crop in China and the world, the harvesting problem of Camellia oleifera has attracted much attention. Research is needed on mechanical characteristics of harvesting equipment. Explicit dynamics was used to establish a finite element model under a simulated load response to the branch-pedicel-fruit system of C. oleifera to predict the fracture process at the pedicel junction. The separation mechanism of C. oleifera fruit was determined by measuring the constitutive parameters of fruit branches and pedicels and conducting separation experiments and explicit dynamics simulations on different hanging fruits. The maximum stress at the fruit pedicel was 1.14 MPa, and the goodness of fit between the simulation and experiment was approximately 89.5%, indicating that the branch-pedicel-fruit finite element model could accurately reflect the fruit shedding process and that the pedicel diameter was correlated positively with the separation force. This study provides technical parameters for the optimized design of existing C. oleifera harvesting equipment.","PeriodicalId":9172,"journal":{"name":"Bioresources","volume":"3 1-2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135463184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Improved method to determine standard values of mechanical properties of original bamboo 原竹力学性能标准值测定方法的改进
4区 农林科学 Q2 MATERIALS SCIENCE, PAPER & WOOD Pub Date : 2023-10-22 DOI: 10.15376/biores.18.4.8374-8393
Jianwei Li, Pengcheng Liu, Haodi Chen, Fangjie Cheng
The standard values of mechanical properties are important performance indexes of original bamboo as a sustainable building material. Such values should be determined by combining the requirement of confidence level and the number of samples. In this paper, systematic tests of longitudinal compression, bending, longitudinal tensile, longitudinal shear, transverse compression, and transverse tensile of bamboo were performed. Based on parametric and non-parametric methods, the influencing factors of the standard values of mechanical properties of bamboo were analyzed. A calculation method and prediction formulas were proposed and the standard values of mechanical properties of bamboo were determined. The results show that the choice of parametric method to calculate the standard value of bamboo strength in the case of a small number of samples may lead to distortion of the results, and the use of non-parametric analysis can effectively reduce the error.
力学性能标准值是原竹作为可持续建筑材料的重要性能指标。该值应结合置信度要求和样本数确定。本文对竹材进行了纵向压缩、弯曲、纵向拉伸、纵向剪切、横向压缩和横向拉伸试验。采用参数法和非参数法,分析了影响竹材力学性能标准值的因素。提出了竹材力学性能的计算方法和预测公式,确定了竹材力学性能的标准值。结果表明,在样本量较少的情况下,选择参数化方法计算竹强度标准值可能导致结果失真,采用非参数化分析可以有效减小误差。
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引用次数: 0
Construction of cement composite using walnut shell reinforced with bacterial nanocellulose gel 细菌纳米纤维素凝胶增强核桃壳水泥复合材料的构建
4区 农林科学 Q2 MATERIALS SCIENCE, PAPER & WOOD Pub Date : 2023-10-22 DOI: 10.15376/biores.18.4.8362-8373
Ali Hassanpoor Tichi, Meysam Razavi
The effect of nanocellulose on mechanical, physical, and morphological properties of composites made of walnut shell and cement was investigated. The mixing ratio of walnut shell as a lignocellulosic material with cement at three levels (10:90, 20:80, and 30:70) and nanocellulose at three levels (0%, 1%, and 3%, based on dry weight of cement) were considered as the variables. Boards were prepared according to the ISO 11925-2 (2020) specifications for the fire resistance properties and according to the DIN EN 634-1 (1995) specifications for the mechanical and physical properties. Morphological properties of composites and nano distribute were evaluated by scanning electron microscopic (SEM) imaging. The results showed that boards containing nanocellulose increased the mechanical properties compared with cement board without nanoreinforcement. The modulus of rupture, modulus of elasticity, and internal bonding of the boards decreased with increased walnut shell amount, and its maximum value was obtained when using 10% walnut shell. Nanocellulose remarkably reduced the fire resistance of the boards. The results from SEM showed that nanocellulose can fill the pores of the composite and create a uniform structure, and thus improved the strength of the boards.
研究了纳米纤维素对核桃壳-水泥复合材料力学、物理和形态性能的影响。作为木质纤维素材料的核桃壳与水泥在三个水平(10:90,20:80和30:70)和纳米纤维素在三个水平(0%,1%和3%,基于水泥的干重)的混合比例被认为是变量。根据ISO 11925-2(2020)耐火性能规范和DIN EN 634-1(1995)机械和物理性能规范制备板材。利用扫描电镜(SEM)对复合材料的形貌和纳米分布进行了表征。结果表明,与未添加纳米增强剂的水泥板相比,添加纳米纤维素的水泥板的力学性能有所提高。随着核桃壳用量的增加,板材的断裂模量、弹性模量和内粘接量均呈下降趋势,当核桃壳用量为10%时达到最大值。纳米纤维素显著降低了板材的耐火性。SEM结果表明,纳米纤维素可以填充复合材料的孔隙,形成均匀的结构,从而提高了复合材料的强度。
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引用次数: 0
Rice bran stabilization using autoclave and optimization of crude rice bran oil recovery using ultrasound-assisted extraction 蒸压釜稳定米糠及超声辅助提取粗米糠油的优化
4区 农林科学 Q2 MATERIALS SCIENCE, PAPER & WOOD Pub Date : 2023-10-22 DOI: 10.15376/biores.18.4.8341-8361
Dodyk Pranowo, Tsamara Dhany Savira, None Sukardi
This research aimed to stabilize rice bran to reduce enzymatic reactions and optimize rice bran oil extraction using ultrasound-assisted extraction (UAE). The optimization was performed using response surface methodology (RSM). The results showed that rice bran oil has three primary fatty acids (oleic, linoleic, palmitic). Stabilization of rice bran using an autoclave (121 °C) for 10 min was recommended, with oil yield (14.2%), free fatty acid/FFA (0.41%), and gamma oryzanol (16.1 ppm). The storage significantly affected oil yield and gamma oryzanol but not FFA (p>0.05). The untreated and stabilized rice bran FFA during 18-day storage ranged from 1.33 to 5.73% and 0.41 to 1.12%. This confirmed that stabilization could inactivate lipase enzymes by reducing FFA. The optimization showed that the linear model best explained oil yield, total phenol content (TPC), and antioxidant activity (IC50), while the 2FI model was best fitted for gamma oryzanol. The optimal condition was found for UAE extraction at an amplitude of 90% and a time of 30 min. Verifying optimal conditions resulted in oil yield (13.6%), TPC (44.8 mg GAE/g), IC50 (207.2 ppm), and gamma oryzanol (15.8 ppm). Further in-depth studies are required to investigate using green solvents in the UAE and gamma oryzanol purification.
本研究旨在稳定米糠,减少酶促反应,优化超声辅助提取米糠油的工艺。采用响应面法(RSM)进行优化。结果表明,米糠油含有油酸、亚油酸和棕榈酸三种主要脂肪酸。推荐使用高压灭菌器(121°C)稳定米糠10分钟,出油率为14.2%,游离脂肪酸/FFA为0.41%,γ -米甲醇为16.1 ppm。贮藏对油脂产量和γ -米甲醇有显著影响,对游离脂肪酸无显著影响(p>0.05)。贮藏18 d时,未处理和稳定的米糠游离脂肪酸含量分别为1.33 ~ 5.73%和0.41 ~ 1.12%。这证实了稳定化可以通过减少FFA来灭活脂肪酶。优化结果表明,线性模型最能解释出油率、总酚含量(TPC)和抗氧化活性(IC50),而2FI模型最能解释γ -米扎酚。最佳提取条件为提取率为90%,提取时间为30 min。对最佳提取条件进行验证,得到出油率(13.6%),TPC (44.8 mg GAE/g), IC50 (207.2 ppm)和γ -米zanol (15.8 ppm)。需要进一步深入的研究,以探讨在阿联酋使用绿色溶剂和γ -米甲醇净化。
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引用次数: 0
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