BMC Research Notes最新文献

This dataset contains demographic, morphological and pathological data, endoscopic images and videos of 191 patients with colorectal polyps. Morphological data is included based on the latest international gastroenterology classification references such as Paris, Pit and JNET classification. Pathological data includes the diagnosis of the polyps including Tubular, Villous, Tubulovillous, Hyperplastic, Serrated, Inflammatory and Adenocarcinoma with Dysplasia Grade & Differentiation.Objectives: Today the most important challenge of developing accurate algorithms for medical prediction, detection, diagnosis, treatment and prognosis is data. ERCPMP is an Endoscopic Image and Video Dataset for Recognition of Colorectal Polyps Morphology and Pathology. This dataset can be used for developing deep learning algorithms for polyps detection, classification, and segmentation.Data description: Images were captured with Olympus colonoscope and are presented in RGB format, JPG type with the resolution of 368 * 256 pixels and 96 dpi. The name of each file (image or video) includes pathological diagnosis, grade and JNet classification of the related polyp.

Objectives: Methicillin-resistant Staphylococcus aureus (MRSA) is a zoonotic pathogen that poses a serious threat to veterinary and public health worldwide. We investigated mastitis milk samples for contamination with MRSA and also characterized the MRSA isolates by investigating antimicrobial resistance and virulence factors.

Result: We confirmed MRSA in 69 of 201 (34.3%) S. aureus isolates recovered from a total of 300 samples. Of the 69 MRSA, 19 (27.5%) were from subclinical cases, while 50 (72.5%) were from clinical cases. The MRSA showed high resistance to penicillin (100%), ampicillin (100%), trimethoprim (69.6%), and tetracycline (69.6%) while susceptibility was observed for gentamicin (100%), vancomycin (95.7%), and ciprofloxacin (91.3%). Most isolates (65.2%, 45/69) were multidrug resistant. Thirteen antibiotypes (A₁-A₁₃) were identified and the most prevalent was A₈ (TMP^RE^RTET^RAMP^RP^R)). All MRSA produced haemolysins, caseinase, and coagulase. Lipase, gelatinase and lecithinase were found in 97.1%, 94.2% and 91.3% of isolates respectively. Genotyping revealed coa (100%) and spa (68.1%) genes. We recommend educating dairy farmers on the public health implications of consuming unpasteurized raw milk and the implementation of proper hygiene practices in dairy farms.

Introduction: The importance of community-based studies is not in doubt, however only few exist because of the complexity and challenges associated with them. Little data exists on these complexities and challenges in West Africa. This study aimed to describe the experiences, challenges and lessons learnt from a community-based Nutritional survey carried out in Nigeria. This was a qualitative review of the experiences and challenges involved in a community-based research project. A thematic content analysis was done and the findings were presented as themes and sub-themes, with the inclusion of quotes that accurately depicted the themes/sub-themes.

Results: Two broad themes emerged from the study. For observations and experiences with community entry, sub-themes include the finding that community structure in the books were different from community structure on the ground, there was a general mistrust among community members, poor access to some communities and/or houses, there was a poor perception of need for the research, there was concern about risk to study participants and about benefit to the participants. The five sub-themes that pertained to the data collection process include the issues with timing, gender, data quality, men being at home and safety of the research team. Guided community engagements are recommended.

Objective: The increase of multidrug-resistant (MDR) strains of Acinetobacter baumannii (A. baumannii), especially carbapenem-resistant strains, is challenging for treating infections. This study investigated the antibiotic resistance pattern and frequency of carbapenem resistance genes (oxacillinase and metallo-beta-lactamase) in A. baumannii.

Results: In this study, 100 bacterial isolates were collected from clinical samples from different hospitals in Isfahan, central of Iran. Of 100 samples of bloodstream, urine, cerebrospinal fluid (CSF), wound, and trachea, 60 bacteria were identified as A. baumannii. The results showed that 100% of the selected isolates were resistant to cefotaxime, ceftazidime, ciprofloxacin, piperacillin-tazobactam, and meropenem. Based on the antibiotic resistance pattern, 25 isolates were chosen for PCR analysis targeting bla_OXA-51, bla_OXA-23, bla_OXA-58, bla_NDM, bla_IMP, and bla_VIM genes PCR results revealed that among the selected isolates, 15 (60.0%) harbored the bla_OXA-23 gene, 23 (92.0%) contained the bla_OXA-51 gene, and 1 (4.0%) isolate carried the bla_NDM gene. Based on MLST analysis, two colistin-resistant Acinetobacter baumannii isolates were categorized as ST2. The ST2 clone represents the predominant sequence type within the CC2 or international clone two. The results showed that the best antibiotic against isolates was colistin. bla_OXA-51 and bla_OXA-23 genes (oxacillinase genes) were dominant genes, but bla_IMP and bla_OXA-58 were not local carbapenem resistant genes in Isfahan.

Objectives: Arboviruses pose a significant global health challenge. This study investigated the seroprevalence of major human arboviral infections, including yellow fever (YFV), dengue (DENV), Crimean-Congo hemorrhagic fever (CCHF), Rift Valley fever (RVF), West Nile virus (WNV), and chikungunya (CHIK), in Darfur region from September to December 2018. ELISA-IgM was used to detect antibodies. RT‒PCR was used to differentiate YFV infection from vaccine-immuno-response in IgM samples.

Results: A total of 152 blood samples were collected, with 123 (80.9%) from males and 29 (19.1%) from females. The participants were grouped by age: 50 (32.9%) were under 20 years, 96 (63.2%) were aged 20-45 years, and 6 (3.9%) were over 45 years. The seroprevalence rates for YFV, DENV, and CHIKV were 68 (44.7%), 23 (15.1%), and 5 (3.3%), respectively. There were 11 molecularly-confirmed YFV cases (7.2%). Among these, 3/11 were positive for DENV-IgM, and 1/11 was positive for CHIKV-IgM. Among the 68 YFV-positive individuals, 15 (22.1%) had been exposed to DENV, and 2 (2.9%) had been exposed to CHIKV. Co-exposure to DENV and CHIKV was detected in 3 (1.9%) patients, while 2 (1.3%) patients had triple exposure to YFV, CHIKV, or DENV. No exposure to CCHF, RVFV, or WNV was detected.

Objective: This study aimed to examine the characteristics of participation in the self-quantification program for family caregivers (CGs) who provide long-term care to community-dwelling older adults. The family CGs, allocated based on the percentage of the nation's older population who needed care and met the inclusion criteria, who provided caregiving at least once a week for those aged 65 + and who were certified as needing care under the Japanese long-term care insurance program, were collected through online monitors. We compared the characteristics of the program participants and nonparticipants using logistic regression.

Results: A total of 2653 family CGs, including 195 study participants who engaged in self-quantification over 60 days and 2,458 nonparticipants who did not engage in self-quantification, were included in the analysis, with complete data available for all variables of interest. The survey included program participants who were predominantly male (55.9%), with an average age of 54.8 years (SD = 10.2). Participants tended to be fully employed (OR = 1.8; p < 0.01), but they were likely to experience greater burdens (OR = 1.8; p < 0.01) and daily caregiving demands (OR = 1.01; p < 0.01). This research highlights the potential efficacy of self-quantification programs for extensively burdened family CGs, illustrating that the requisites for support vary in accordance with the distinct characteristics of these CGs.

Fibrates can prevent and treat ischemic stroke (IS), the occurrence and development of IS is closely related to hypoxia-inducible factor-1A (HIF-1A). However, the exact mechanism by which fibrates regulate HIF-1A to treat IS remains unclear. So network pharmacology and molecular docking were used to explore the mechanism by which fibrates regulate HIF-1A to treat IS, firstly, the structure of five fibrates were obtained by reviewing the literature and pharmacopoeia, then the potential targets of fibrates, IS, HIF1A and HIF1A-related genes were obtained through various databases, their common targets were obtained through Venny 2.1.0. The PPI network diagram of fibrates, IS and HIF1A-related genes was plotted by String and Cytoscape3.8.1. The GO functional analysis results and KEGG pathways of fibrates, IS, HIF1A and HIF1A related genes were obtained by Metascape. Finally, the molecular docking of fibrates and HIF1A was performed by AutoDock. The common targets of five fibrates and IS showed that only 3 fibrates contained HIF1A, GO functional analysis, KEGG pathway analysis and molecular docking showed that fibrates can better regulate HIF1A to treat IS, its main action pathways are pathways in cancer, lipid and atherosclerosis and HIF-1 signaling pathway.

Objective: Hypertension exerts a substantial financial burden on individuals, families, communities, and the health system of a country. The current study aimed to describe the association of hypertension with its main risk factors in the Afghan population living in Kandahar city.

Results: The prevalence of tobacco smoking, naswar use, physical activity and a healthy diet was 22%, 55%, 63.9% and 33.6%, respectively, in men, while in women, the prevalence was 4.8%, 6.5%, 57.6% and 13.5%, respectively. The prevalence of hypertension was 28.2% and 23.9% in men and women, respectively. Hypertension was associated with age greater than 45 years [aOR (95% CI) = 2.13 (1.56-2.91)], widows [aOR (95% CI) = 1.91 (1.25-2.91)], medium and high monthly income [aOR (95% CI) = 3.45 (2.33-5.10) and aOR (95% CI) = 2.34 (1.50-3.64)], high physical activity [aOR (95% CI) = 1.72 (1.15-2.56)], obesity [aOR (95% CI) = 1.64 (1.16-2.34)] and family history of hypertension [aOR (95% CI) = 4.70 (2.44-9.04)]. Naswar use is more prevalent than tobacco smoking among urban residents from Kandahar province. Improved monitoring and control of risk factors for hypertension are required in all regions of Afghanistan.

Objective: The objective of this study is to synthesize and comprehensively characterize a novel iodine-containing coordination compound, di-aminopropionic acid hydrogen tri-iodide. This involves determining its structural, physicochemical, and thermal properties, as well as evaluating its antimicrobial activity against a range of bacterial strains, including multidrug-resistant pathogens. The aim is to explore the potential of this compound as a candidate for developing new antibacterial agents to address the challenge of antibiotic resistance.

Results: An original iodine-containing semiorganic coordination compound was synthesized and characterized. The physicochemical properties were studied via diffraction, FTIR spectroscopy and thermogravimetric/differential scanning calorimetry (TG/DSC). In vitro antimicrobial activity evaluation was performed using two-fold serial dilution method. The obtained results demonstrated efficiency against both gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii) bacteria including MDR strains that cause infectious disease. The results of the antibacterial evaluation revealed that the new iodine coordination compound - di-aminopropionic acid hydrogen tri-iodide possesses high bactericidal properties and exhibits better antimicrobial activity against both susceptible and multidrug-resistant strains than does Lugol solution and ampicillin sodium, which are used as reference drugs.