Brazilian oral research最新文献

Angiotensin II (Ang II) releases inflammatory mediators from several cell types. The objective of this study was to investigate the potential of Ang II to induce mRNA expression of inflammatory mediators in primary cultured fibroblast-like cells isolated from gingival and periodontal ligament tissues. A synergistic effect of co-treatment with Ang II and Interleukin-1β (IL1β) on the mRNA expression of inflammatory mediators was explored. Immunophenotyping of STRO-1, Ang II type 1 receptor (AT1R), and Ang II type 2 receptor (AT2R) was performed using flow cytometry. Cell cultures were challenged with Ang II (1 µM) for 3, 6, and 24 h with or without co-treatment with IL1β (0.1 ng/mL) for 24 h. mRNA expression of inflammatory mediators was determined using qPCR. We present, for the first time, precise quantification of AT1R and AT2R in human gingival and periodontal fibroblast-like cell types; the percentage of positive immunostaining compared to the total cell population varied from 3.35% to 5.29% for AT1R and 2.97% to 4.57% for AT2R. Ang II slightly upregulated IL6 and CCL2/MCP1 mRNA expression in gingival cells and IL8 and PTGS2/COX2 in periodontal ligament cells. IL1β upregulated IL8, IL6, CCL2/MCP1, PTGS2/COX2, and IL1β mRNA in both cell types. Co-treatment with Ang II and IL1β did not show a synergistic effect. Ang II showed a low potential to induce mRNA of inflammatory mediators, most likely owing to the low percentage of Ang II receptors in such cells and no synergistic effect with the co-treatment with IL1β.

The purpose of our review was to group the evidence and attempt to provide a consensus on the behavior of salivary flow rate in patients with Down syndrome. Observational studies evaluating salivary flow rate in children and teenagers with Down syndrome compared with non-syndrome individuals were selected. Ten sources of information were researched. The risk of bias was assessed by using the Newcastle Ottawa Scale tool . Inverse Variance was ty the SMD (95% Confidence Interval). The certainty of the evidence was determined according to the GRADE approach. Fourteen studies were evaluated. The results showed, with a very low certainty of evidence, that children and teenagers with Down syndrome present a lower salivary flow rate compared with non-syndrome controls (SMD: -1.71, 95%IC: -2.81; -0.60, p < 0.05), with significant differences in the saliva collection methods (p < 0.05) (Unstimulated saliva, SMD -5.07, 95%CI: -7.96; -2.18, p < 0.01; Stimulated saliva, SMD -0.80, 95%IC: -1.78; 0.17, p = 0.11). The behavior of the salivary flow rate is not significantly different between the age groups (p = 0.60) (up to 5 years old, SMD -1.85, 95%CI: -2.90; -0.81, p < 0.01; 2 to 18 years old, SMD -1.51, 95%CI: -2.24; -0.78, p < 0.01), and the sex (p = 0.70) (Male, SMD -1.77, 95%CI: -2.39; -1.16, p < 0.01; Female, SMD -1.53, 95%CI: -2.58; -0.48, p < 0.01). Children and teenagers with Down syndrome present a lower salivary flow rate with an unstimulated saliva collection method compared to non-syndrome.

Tumor necrosis factor-alpha (TNF-α) is a cytokine involved in the immune-inflammatory response. It can induce an odontoblastic phenotype and enhance biomineralization in dental pulp mesenchymal stem cells but does not have the same effect on osteoblasts. The reasons for this differential response, despite the shared lineage of these cell types, are not yet clear. This study examined the effects of TNF-α on immortalized mouse dental pulp stem cells (OD-21) and pre-osteoblastic cells (MC3T3-E1). Cells were treated with recombinant TNF-α at concentrations of 1, 10, and 100 ng/mL. Cell viability, proliferation, and migration were assessed using the MTT, CyQUANT, and wound healing assays, respectively. Gene expression was assessed via real-time RT-PCR, and biomineralization was evaluated using alizarin red staining. Statistical analysis was conducted using one-way ANOVA followed by Tukey's post-hoc test (α = 0.05). TNF-α did not affect cell viability at any concentration (p > 0.05). Proliferation and migration increased after 12 h, with near-complete wound closure by 24 h. TNF-α promoted proliferation and migration in both cell types. OD-21 cells exhibited high levels of Tnfr1 and Runx2 expression and showed biomineralization. In contrast, MC3T3-E1 cells showed high Tnfr2 levels, suppressed Runx2, and inhibited biomineralization. These results highlight how TNF-α influences different cell types from the same lineage in distinct ways.

The aim of this in-vitro study was to verify which field of view (FOV) in cone-beam computed tomography (CBCT) yields greater accuracy in the detection of internal root resorption (IRR) volume, in comparison to the gold standard of micro-computed tomography (micro-CT) and to a physical method. Twenty-five extractedsingle-rooted teeth were scanned by CBCT with two different FOV parameters (6x6-FOV and 10x10-FOV) and via micro-CT. The volume of dental hard tissue was measured on these images. A simulated IRR was produced by a demineralization protocol. After the simulated IRR, the volumes of the dental hard tissue and the simulated IRR were measured with the same scanning parameters. In addition, the volume of the simulated IRR was measured via a physical method. The simulated IRR volumes obtained by CBCT, micro-CT, and the physical method were statistically compared using one-way ANOVA. Before the simulated IRR, the mean volume of dental hard tissue obtained by 6x6-FOV, 10x10-FOV, and micro-CT were 266.64 ± 11.56, 284.78 ± 14.99, and 233.07 ± 19.91, respectively. The simulated IRR mean volumes obtained by 6x6-FOV, 10x10-FOV, micro-CT, and the physical method were 19.35 ± 5.92, 17.43 ± 5.20, 23.85 ± 6.63, and 13.51 ± 3.11, respectively. The mean volume of the simulated IRR obtained by micro-CT was similar to that of the 6x6-FOV and was significantly different from that of the 10x10-FOV and physical method. The mean volume value of simulated IRR obtained by the physical method was significantly different from those of the micro-CT and 6x6-FOV groups. In conclusion, the 6x6-FOV was better than the 10x10-FOV for the detection of IRR volume by CBCT under clinical conditions.

The aim of the study was to explore female authorship in various aspects of randomized controlled trials (RCTs) in dentistry. A search was performed in PubMed for RCTs, in dentistry, indexed from 12/31/2016 to 12/31/2021. Only studies in English were considered. Data selection and extraction were performed by two authors and the following data collected: year of publication, journal, subject, number and names of authors, and country and gender (Genderize website) of the first 10 authors. Descriptive analyses, graphs, and maps were generated. Poisson regression assessed the influence of continent and year of publication on the presence of women as first or last authors. The results were presented as prevalence ratios (PR) and 95% confidence intervals (95%CI). A total of 844 RCTs and 4,305 authors (2,372 men and 1,662 women) were included. Gender disparity increases as the order of authorship advances. Among first authors, men represent 50.59% and women 44.08%, whereas among last authors, they account for 61.92% and 34.03%, respectively. Analyses showed no association between year of publication and the presence of women as authors. There were fewer women as first authors in Europe (PR: 0.82, 95%CI: 0.68-0.99) and as last authors in Europe and Asia (PR: 0.68, 95%CI: 0.53-0.87 and PR: 0.79, 95%CI: 0.63-0.99, respectively). The findings highlight a lower presence of female authors in all aspects analyzed in the RCTs, especially in last authorship. Also, there has been no indication of improvement in recent years. Female participation in RCTs is crucial not only for gender equity but also as a means to enhance the quality and relevance of clinical data for decision-making.

This study aimed to histomorphometrically evaluate the effect of guided bone regeneration (GBR) and two implant surfaces on the thickness and height of newly formed bone in dehiscence defects around titanium implants. Three premolars and the first bilateral molar were extracted from ten adult mongrel dogs, and 40 buccal bone dehiscences measuring 5 mm in height and 4 mm in width were created using a University of North Carolina (UNC) periodontal probe to confirm the dimensions. Forty implants were randomly assigned to one of four groups: oxidized implant surfaces (OIS, n = 10), turned/machined implant surfaces (TIS, n = 10), OIS + GBR (n = 10), and TIS + GBR (n = 10). After 3 months, the dogs were euthanized, and the blocks containing the implants and adjacent bone were processed for non-decalcified histological analysis. Statistical analysis was performed using two-way ANOVA and the Pearson correlation (p = 0.05). The results showed that GBR significantly influenced both the vertical (height) and horizontal (thickness) dimensions of the newly formed bone (p < 0.001). Strong positive correlations were observed between the thickness and height of newly formed bone at the base of the defect, as well as between the thickness of the bone at the base of the defect and the thickness of newly formed bone in the central region of the defect. No significant correlations were found between implant surface type and bone formation. Bone regeneration occurred in both the vertical and horizontal directions, with greater bone growth in GBR-treated groups, irrespective of implant surface type (oxidized or turned).

The contamination of dental curing light tips was evaluated before and after treatment and after their use and disinfection. The influence of a plastic protective barrier over the flexural strength and the modulus of elasticity of resin composites were also analyzed. Microbiological sampling was conducted at initial contamination (T0), in Log 10 CFU/4 mL; after dental treatment (T1); and after disinfection with 70% ethanol (v/v) (T2). The results were analyzed by descriptive statistics and analysis of variance. The flexural strength and the modulus of elasticity analyses were performed using bar-shaped test specimens of three commercial resin composites with and without barriers, and the tests were subjected to a statistical normality test. Turbidity was observed in the media in 60.7% of the tubes at T0, 100.0% at T1, and 57.1% at T2. The microbial contamination was similar at T0 and T2, but a significant increase was observed at T1. The recovered microbial load differed significantly between T1 and T2 (p < 0.05). The results of the flexural strength and modulus of elasticity analyses showed no significant differences with or without a barrier for any of the different colors of resin, polymerization time, or the three resins. Under the present experimental conditions, 70% ethanol significantly reduced the levels of microbial contamination, but it did not guarantee the inactivation of all microbial cells. The use of plastic protective barriers did not alter the flexural strength or the modulus of elasticity of any of the tested resin composites, indicating that they are a safe and viable measure to prevent cross-contamination when using a dental curing light.

Advancements in digital media have driven the study and use of photographic records as a diagnostic method for carious lesions, with smartphone images being widely utilized across various health fields. This study aimed to evaluate the diagnostic accuracy of smartphone photography for detecting active caries in orthodontic patients. The sample comprised 100 individuals of both sexes, aged 11 to 46 years, who were undergoing fixed orthodontic treatment. Following professional tooth cleaning and drying, patients underwent a visual-tactile clinical examination for caries detection, which served as the gold standard. Digital photographs were then taken using a smartphone camera in five views: frontal, right and left lateral, and upper and lower occlusal. The diagnostic parameters-sensitivity, specificity, accuracy, positive predictive value (PV+), and negative predictive value (PV-)-were calculated for the photographic method relative to the clinical examination. The prevalence of active caries in the sample was 74%. The smartphone photographs correctly identified 66 of the 74 individuals with active caries according to the gold standard. However, only 4 of the 26 sound patients were correctly identified. These findings correspond to the following diagnostic parameters: sensitivity, 89%; specificity, 15%; accuracy, 70%; PV+, 75%; and PV-, 33%. In conclusion, the photographic method demonstrated high sensitivity and satisfactory accuracy in detecting caries in patients undergoing fixed orthodontic therapy. However, the low specificity observed suggests a tendency to overdiagnose sound teeth.

In this cross-sectional study, carried out in 2012, we assessed factors associated with tooth loss among adolescents from Minas Gerais, Brazil, utilizing data from a secondary database. Individual and local-level variables were selected to represent health determinants. Individual covariates included sex, age, skin color, maternal education, household income, use of dental services, and self-perceived need for dental care. The contextual variables included illiteracy, unemployment, income, primary health care coverage, dental specialty centers, and oral health team coverage. Multilevel logistic regression models were used to examine the relationship between contextual and individual variables and the outcome (STATA version 16.0) - odds ratio (OR) and 95% confidence interval (CI). The prevalence of tooth loss was 12.4%. Female individuals were 40% more likely to experience the outcome (OR: 1.40; 95%CI: 1.01-1.98). Increased age was associated with greater tooth loss (OR: 1.16; 95%CI: 1.03-1.31). Low maternal education (OR: 1.72; 95%CI: 1.13-2.61), low household income (OR: 1.71; 95% CI 1.09-2.67), and self-perceived dental needs (OR: 2.94; 95% CI 1.97-4.39) were also associated with the outcome. Regular dental visits reduced the likelihood of tooth loss by 38% (OR: 0.62; 95 CI 0.44-0.87). Larger tooth loss was observed in municipalities with higher illiteracy rates (OR: 1.04; 95%CI: 1.01-1.08). Municipalities with larger dental specialty centers were associated with the outcome (OR: 0.58; 95%CI: 0.37-0.92). Contextual and individual factors influenced tooth loss in adolescents from Minas Gerais, Brazil, but socioeconomic status was the main determinant.

This study aimed to determine the prevalence and provide an overview of Down syndrome and child- and mother-associated factors in Brazil from 2010 to 2020. This was a cross-sectional study including epidemiological characteristics related to live births of individuals with and without Down syndrome using the Brazilian government website. The average prevalence of Down syndrome in Brazil was approximately 30.4 children per 100,000 live births during the 10-year period, corresponding to 1 case in every 3,289.47 newborns. The occurrence of Down syndrome was increased in White children, preterm births, and low birth weight infants compared to the no-Down syndrome group; however, it was decreased in males. The prevalence of Down syndrome was low among mothers without a spouse, insufficient prenatal care, and vaginal delivery. Conversely, the prevalence was high among mothers aged 35 years and older and mothers considered White. There was no evidence of a time correlation in the prevalence among the regions of Brazil during this 10-year period. It is imperative to strengthen national monitoring of the prevalence of children with Down syndrome and provide better support to mothers and public services for this group.