Brazilian oral research最新文献

This systematic review aims to provide preclinical evidence of the antimicrobial efficacy of natural endodontic solutions (NES) compared to sodium hypochlorite (NaOCl) and chlorhexidine (CHX) against Enterococcus faecalis. The study followed the PRISMA guidelines and had a registered protocol (PROSPERO - CRD42021224022). The inclusion criteria comprised ex vivo studies simulating root canal irrigation to assess the standardized mean difference of colony-forming units (CFUs). Records were systematically identified in PubMed, Scopus, EMBASE, SciELO, Web of Science, LILACS, DANS Easy, and BDTD databases in January 2024. The RoBDEMAT tool helped determine the risk of bias. As for the network meta-analysis, CFU data were collected pre- and post-irrigation protocols as an effect measure. Thirty-five studies met the eligibility criteria and were included in the qualitative analysis, and seven studies were eligible for the network meta-analysis. The total sample consisted of 1,504 root canals. Among the tested solutions, apple cider vinegar combined with 2.5% NaOCl showed the highest probability of reducing CFUs after root canal irrigation (95%CI: -0.76-0.20), followed by 2% CHX and NaOCl (considering the different concentrations [95%CI: -0.32-0.17]). The bias assessment revealed significant omissions in reporting, particularly regarding sample size calculation, sample randomization, and operator blinding. The obtained evidence demonstrated that, even though NES did not overcome NaOCl, they have antimicrobial potential against Enterococcus faecalis.

To exclusively evaluate, in vitro, the efficacy of five intracanal medicaments against Candida albicans and Enterococcus faecalis in infected single-rooted primary teeth. Forty-three teeth were selected, out of which 42 were simultaneously contaminated with C. albicans and E. faecalis, verified by measuring the initial quantification (IQ), whereas one tooth was used as sterile control. Forty-two teeth were then randomly assigned to six groups (n = 7 teeth in each); one was the control group, while the others received calcium hydroxide (CH), camphorated paramonochlorophenol (CMCP), CH/CMCP, chlorhexidine (CHX), or iodoform paste (IP). Two samples from each group were then randomly selected and evaluated under a scanning electron microscope along with the sterile control (n = 1) for qualitative analyses (n = 13). The remaining samples (n = 30) were collected after 7 days of intracanal medicaments (final quantification - FQ) and 7 days after medicament removal (residual quantification - RQ). ANOVA and Tukey's and c2 tests (p £ 0.05) were performed for the statistical analysis. All the medicaments, except CMCP, significantly reduced the levels of C. albicans from IQ to FQ (p = 0.046). Only CHX (p = 0.000) and IP (p = 0.007) showed a significant reduction from IQ to FQ against E. faecalis. The 2% CHX and IP demonstrated a favorable performance as an intracanal medicament, with a good antimicrobial activity against C. albicans and E. faecalis.

Curcumin, found in turmeric rhizomes (Curcuma longa L.), has been widely studied for its potential health benefits, including anti-inflammatory, antioxidant, and wound-healing properties. However, due to its low bioavailability and unfavorable pharmacokinetics, analogous compounds have been developed to obtain better biopharmaceutical characteristics and enhanced biological effects. In this study, we evaluated the activity of curcumin and three of its synthetic analogues (DMAD, DMAM, and RI75) on the viability and differentiation of a pre-osteoblastic cell line (MC3T3-E1). We also assessed the expression of key genes involved in tissue regeneration: vascular endothelial growth factor (vegf), stromal-derived growth factor 1 (SDF-1/CXCL12), and runt-related transcription factor 2 (runx2). The cells were treated with curcumin and the three analogues at concentrations of 10, 30, or 50 μM. All tested analogues and curcumin exhibited moderate to no cell toxicity compared to the cells treated under standard conditions across all concentrations after 24, 48, and 72 hours. Only the RI75 analogue showed upregulation of SDF-1, a crucial factor in tissue regeneration. Compared to curcumin, the DMAM and RI75 analogues also upregulated runx2 and vegf, both associated with osteodifferentiation. The RI75 analogue demonstrated greater mineralization than curcumin, and both promoted more nodule formation than the untreated control. Our data suggest that the curcumin analogue RI75 at 50 μM presents similar toxicity but enhanced biological activity compared to natural curcumin, making it a promising substance for material biomodifications.

This study compared the effects of different final irrigation protocols on the mechanical properties and structural integrity of root dentine. One-hundred eight teeth were instrumented and irrigated with sodium hypochlorite (NaOCl) using conventional irrigation (CI). Teeth were distributed into four groups based on final irrigation protocols: Control Group (CG): 17%EDTA/CI + H2O; G1: 2.5%NaOCl/Passive Ultrasonic Irrigation (PUI) + EDTA/PUI + NaOCl/PUI (60s each); G2: EDTA/PUI + NaOCl/PUI (30s each); G3: EDTA/PUI + NaOCl/PUI + H2O/PUI + CHX/PUI (30s each). Four tests were conducted: three-point flexural strength test, Scanning Electron Microscopy analysis, microhardness assessment, and the push-out bond strength (POBS) of the filling material to the root dentine. Data were analyzed using ANOVA and Tukey tests (Flexural strength), and Student t-test (Microhardness). Erosion scores and POBS were analyzed by Kruskal-Wallis and Dunn tests (α = 0.05). Results indicated no significant differences in flexural strength (p > 0.05) among groups. CG exhibited the lowest erosion scores in the cervical third. In the middle third, CG had lower scores than G1 and G2, while in the apical third, CG had lower scores than G1 (p < 0.05). Microhardness values decreased following the protocols (p < 0.05), except for the CG (p > 0.05). G2 displayed higher POBS values in the middle and apical thirds (p < 0.05). The protocols did not significantly influence the flexural strength of root dentine. However, they did affect microhardness and promoted greater erosion. The best results for POBS were observed when the final irrigation involved the sequential use of EDTA and NaOCl employing PUI for 30 seconds in each solution.

The purpose of this study was to evaluate the effect of a single dose of ionizing radiation (30 Gy) on the osseointegration of implants in the rabbit tibia. Twenty rabbits received two dental Morse-tapered junction implants and one implant in each tibia. The animals were randomly divided into two groups (n=10), non-irradiated (NoIr) and irradiated (Ir), wherein the Ir group received a single dose of 30 Gy radiation 2 weeks after implant installation. Microtomographic analyses (BV/TV) and histomorphometric assessments (BIC and BABT) were performed 4 weeks after implant installation. One-way ANOVA, Tukey's test, and Student's t-test (α=0.05) were used for data analysis. The results showed that BV/TV did not differ significantly between the Ir and NoIr groups (P = 0.071). In the histomorphometric analysis, neither BIC nor BABT showed significant differences between the NoIr and Ir groups (p>0.05). In conclusion, ionizing radiation in dental implants does not appear to interfere with osseointegration when installed prior to irradiation.

There is an interaction between dendrocytes and mast cells in the skin. However, in elastosis-related diseases such as actinic cheilitis (AC) and lower lip squamous cell carcinoma (LLSCC), this interaction remains unknown. We investigated the presence of intact and degranulated mast cells in AC and LLSCC. Associations of mast cells with factor XIIIa+ dendrocytes and inflammatory infiltrate were assessed. Forty cases of AC (20 with low-grade and 20 with high-grade epithelial dysplasia), 50 cases of LLSCC, and 10 cases of normal oral mucosa were evaluated. Toluidine blue staining was performed to identify mast cells, and mast cell densities were calculated in the inflammatory infiltrate. Factor XIIIa+ dendrocytes were immunohistochemically quantified. The highest ratio of intact/degranulated mast cells density was detected in LLSCC (5.9 cells/mm2), followed by AC with high-grade epithelial dysplasia (4.8 cells/mm2). Statistically significant differences were found in the density of intact mast cells compared to degranulated mast cells in AC with low-grade epithelial dysplasia (p<0.001), AC with high-grade epithelial dysplasia (p=0.005), and LLSCC (p<0.001). A positive correlation between degranulated mast cells and total inflammatory infiltrate (p=0.03) was observed in the LLSCC group. The expression of factor XIIIa+ dendrocytes was highest in AC with low-grade epithelial dysplasia (16.5 cells/mm2). The link between mast cell density, factor XIIIa+ dendrocytes, and inflammatory infiltrate indicates a potential crosstalk in lip carcinogenesis.

The Colombian Chapter of the Alliance-for-a-Cavity-Free-Future (Col-ACFF) has been conducting a health promotion and caries prevention program among young children in four vulnerable Colombian municipalities (baseline data from 2012-2014). This study aimed to quantify socioeconomic inequalities in early childhood caries (ECC) and examine the potential role of daily fluoride-toothpaste use, previous-year dental-care visit, and nutrition/diet-related aspects. The study sample included 1344 children aged 1-5 years. Inequalities in the age-standardized prevalence rates of and mean number of tooth surfaces affected by moderate/extensive (dME) and initial (dIME) caries (defined using the ICDAS-merged-epi criteria) by household income and level of education were examined using the relative index of inequality (RII) and the slope index of inequality (SII). Approximately one-third of the children included in this study exhibited dME, while 84% exhibited dIME. The majority of outcomes exhibited social gradients, and significant relative (RII) and absolute (SII) inequalities in ECC were observed. The SII estimate indicated an absolute difference of 12.4% in the prevalence of moderate/extensive carious lesions among children living in households with the lowest compared to the highest education levels [SII: 12.4; 95% confidence interval (CI): 2.7-22.1]. These children were also 6.7 times more likely to exhibit dIME compared to those living in households with higher levels of education (SII:6.73 95% CI: 4.18-9.29). Daily use of fluoride toothpastes, dental care visits in the previous year, and nutrition/diet-related factors played a limited role in ECC inequalities. In conclusion, significant ECC inequalities were observed in these vulnerable populations, highlighting the importance of upstream and downstream interventions that raise awareness among stakeholders and improve community- and individual-based practices to address this.

The study assessed the Global Quality Score (GQS) and informational engagement of users with YouTube videos on the Brazilian public health system (SUS). The YouTube video search tool was used with the Portuguese keywords 'unified health system' and 'SUS'. The first 100 videos returned in the search were studied, using the GQS to measure their educational value, usefulness, and information quality. Users' engagement with the videos was calculated based on their number of likes/reactions and comments. Other data collected were authorship, year of publication, topic approached, target audience, video length, and use of references. Two trained and calibrated researchers collected the data. Multiple analysis was performed with Logistic Regression, using a 95% confidence interval and significance of p<0.05. There were no poor or generally poor GQS scores (scores 1 and 2) and most videos (58%) achieved moderate or good scores (scores 3 and 4). Videos published after the onset of COVID-19 had a 70% lower chance of engagement than those published in pre-pandemic years (OR: 0.30; 95%CI: 0.12-0.74). Videos that targeted healthcare professionals were 72% less likely to achieve higher GQS scores, than those with an unidentified target audience (OR: 0.28; 95%CI: 0.10-0.75). The informational engagement of the videos showed fewer comments than likes/reactions. Most YouTube videos about the SUS had moderate or good global quality, which was associated with their period of publication and choice of target audience.

This study evaluated the scientific contribution of Brazilian CNPq Research Productivity fellows (PQ) in Dentistry by areas of activity. This cross-sectional study included 217 active PQ who were grouped into six groups: (1) Biomaterials, Prosthodontics, and Restorative Dentistry; (2) Public Health and Epidemiology; (3) Oral Pathology, Stomatology, and Dental Radiology; (4) Pediatric Dentistry and Child Health; (5) Dental Clinic (Periodontics, Endodontics, Orthodontics, Oral Surgery, and Implantology), and (6) Basic Areas (Histology, Biochemistry, Molecular Biology, Physiology, Microbiology, Immunology, and Pharmacology). The PQ were predominantly male (n = 122; 56.2%), received level 2 scholarships (n = 121; 55.8%), and performed research in the Southeast region of Brazil (n = 160; 73.73%). Regarding supervision of undergraduate, master's, and PhD students, both during their entire careers and in the last 5 years, the highest average was observed for PQs in the field of Public Health and Epidemiology, the only area with higher average supervision of master's than that of undergraduate and PhD students. PQ in Public Health and Epidemiology had the highest average number of papers published over their career and in the last 5 years, followed by PQ in Pediatric Dentistry and Child Health and Dental Clinic. The high productivity of PQ is demonstrated by modern research performance indicators. Their scientific publications are indexed in bibliometric databases such as WoS, Scopus, and SciELO. Addtionally, highlighted among the PQ was the time since initiation of their scientific careers and master's and doctoral candidates trained.

This cross-sectional study aimed to analyze the self-perceived oral health of young university students at the Federal University of Juiz de Fora and identify the associated factors. Data were collected in 2021 using a self-administered questionnaire containing questions on students' sociodemographic variables and oral health. Binary logistic regression was applied in the multivariate analysis using the SPSS (Statistical Package for the Social Sciences) software (version 20.0) for Windows. The final analysis included 1,316 students aged 17-24 years. The prevalence of negative self-perception of oral health was 14.1% (95%CI: 12.2-16.0). The following variables were associated with negative self-perception of oral health: single marital status (OR = 0.34; 95%CI: 0.12-0.98), monthly family income of up to three minimum wages (OR = 2.02; 95%CI: 1.32-3.09), non-regular use of dental services (OR = 2.29; 95%CI: 1.48-3.53), dissatisfaction with the last service (OR = 1.97; 95%CI: 1.23-3.16), fear of dental treatment (OR = 1.56; 95%CI: 1.06-2.29), dissatisfaction with the appearance of teeth and mouth (OR = 5.27; 95%CI: 3.37-8.22), and perceived need for dental treatment (OR = 6.94; 95%CI:3.14-15.33). In conclusion, most young university students had a positive self-perception of oral health. However, factors related to socioeconomic profile, access to oral health services, and satisfaction with one's appearance were found to increase the likelihood of having a negative self-perception of oral health.