Pub Date : 2024-04-05eCollection Date: 2024-01-01DOI: 10.1590/1807-3107bor-2024.vol38.0031
Cláudia Callegaro de Menezes, Davi da Silva Barbirato, Mariana Fampa Fogacci, Guido Artemio Marañón-Vásquez, João Régis Ivar Carneiro, Lucianne Copple Maia, Maria Cynésia Medeiros de Barros
This systematic review aimed to answer the focused question: "What are the benefits of subgingival periodontal therapy on blood hematological and biochemical index, biomarkers of inflammation and oxidative stress, quality of life, and periodontal pathogen counts in patients with obesity and periodontitis?". A systematic literature search was performed in six databases: PubMed, Embase, LILACS, Web of Science, Cochrane and SCOPUS and other sources, and a manual search was conducted as well. Inclusion criteria were randomized and non-randomized clinical trials, and before-and-after studies on patients with obesity subjected to periodontal therapy. The results were synthesized qualitatively. Risk of bias within studies was assessed using RoB 2 and ROBINS-I tools. The certainty of evidence was evaluated following the GRADE approach. Three randomized controlled trials and 15 before-and-after studies were included. Randomized controlled trials were considered to have a low risk of bias, as compared to before-and-after studies assessed as having low, serious, and critical risks of bias. Non-surgical periodontal therapy plus azithromycin, chlorhexidine, and cetylpyridinium chloride reduced blood pressure and decreased serum levels of HbA1c, hsCRP, IL-1β, and TNF-α. Salivary resistin level also decreased in patients with obesity and periodontitis after therapy and chlorhexidine mouth rinse. Before-and-after data suggest an improvement in total cholesterol, LDL, triglycerides, insulin resistance, C3, GCF levels of TNF-α, chemerin, vaspin, omentin-1, visfatin, 8-OHdG, and periodontal pathogen counts after therapy.
{"title":"Systemic benefits of periodontal therapy in patients with obesity and periodontitis: a systematic review.","authors":"Cláudia Callegaro de Menezes, Davi da Silva Barbirato, Mariana Fampa Fogacci, Guido Artemio Marañón-Vásquez, João Régis Ivar Carneiro, Lucianne Copple Maia, Maria Cynésia Medeiros de Barros","doi":"10.1590/1807-3107bor-2024.vol38.0031","DOIUrl":"https://doi.org/10.1590/1807-3107bor-2024.vol38.0031","url":null,"abstract":"<p><p>This systematic review aimed to answer the focused question: \"What are the benefits of subgingival periodontal therapy on blood hematological and biochemical index, biomarkers of inflammation and oxidative stress, quality of life, and periodontal pathogen counts in patients with obesity and periodontitis?\". A systematic literature search was performed in six databases: PubMed, Embase, LILACS, Web of Science, Cochrane and SCOPUS and other sources, and a manual search was conducted as well. Inclusion criteria were randomized and non-randomized clinical trials, and before-and-after studies on patients with obesity subjected to periodontal therapy. The results were synthesized qualitatively. Risk of bias within studies was assessed using RoB 2 and ROBINS-I tools. The certainty of evidence was evaluated following the GRADE approach. Three randomized controlled trials and 15 before-and-after studies were included. Randomized controlled trials were considered to have a low risk of bias, as compared to before-and-after studies assessed as having low, serious, and critical risks of bias. Non-surgical periodontal therapy plus azithromycin, chlorhexidine, and cetylpyridinium chloride reduced blood pressure and decreased serum levels of HbA1c, hsCRP, IL-1β, and TNF-α. Salivary resistin level also decreased in patients with obesity and periodontitis after therapy and chlorhexidine mouth rinse. Before-and-after data suggest an improvement in total cholesterol, LDL, triglycerides, insulin resistance, C3, GCF levels of TNF-α, chemerin, vaspin, omentin-1, visfatin, 8-OHdG, and periodontal pathogen counts after therapy.</p>","PeriodicalId":9240,"journal":{"name":"Brazilian oral research","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140859793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-05eCollection Date: 2024-01-01DOI: 10.1590/1807-3107bor-2024.vol38.0024
Lorena Souza Santos Mariano, Rafael Nakamura-Silva, Luciana Martins Domingues de Macedo, Mariana de Oliveira-Silva, Rafael da Silva Goulart, Marsileni Pelisson, Eliana Carolina Vespero, Yara Teresinha Correa Silva-Sousa, André Pitondo-Silva
This study aimed to identify and characterize the antimicrobial susceptibility profile of bacteria found in primary endodontic infections in the teeth of patients treated at the Dental Clinic of the University of Ribeirão Preto, São Paulo, Brazil. From September to December 2019, samples were obtained from 21 patients with primary endodontic infections. The collections were carried out in triplicate using paper cones placed close to the total length of the root canal. Bacterial isolation was performed in Brain Heart Infusion agar, Blood agar, and other selective culture media cultured at 37°C for up to 48 h under aerobiosis and microaerophilic conditions. The bacterial species were identified using the Vitek 2 automated system. The disk diffusion method on agar Müeller-Hinton was used to assess antimicrobial susceptibility with the recommended antimicrobials for each identified bacterial species. A total of 49 antibiotics were evaluated. Fifteen of the 21 samples collected showed bacterial growth, and 17 bacterial isolates were found. There were 10 different bacterial species identified: Enterococcus faecalis (four isolates), Streptococcus mitis/oralis (three isolates), Streptococcus anginosus (three isolates) being the most common, followed by Staphylococcus epidermidis, Enterococcus faecium, Streptococcus constellatus, Streptococcus alactolyticus, Enterobacter cloacae, Klebsiella variicola, and Providencia rettgeri (one isolate of each species). The analysis demonstrated significant susceptibility to most of the tested antibiotics. However, some Enterococcus isolates resisted the antibiotic's erythromycin, ciprofloxacin, and tetracycline. A Staphylococcus epidermidis isolate was characterized as multidrug-resistant. Five Streptococcus isolates were non-susceptible to all antibiotics tested.
{"title":"Identification and antimicrobial susceptibility profile of bacteria isolated from primary endodontic infections.","authors":"Lorena Souza Santos Mariano, Rafael Nakamura-Silva, Luciana Martins Domingues de Macedo, Mariana de Oliveira-Silva, Rafael da Silva Goulart, Marsileni Pelisson, Eliana Carolina Vespero, Yara Teresinha Correa Silva-Sousa, André Pitondo-Silva","doi":"10.1590/1807-3107bor-2024.vol38.0024","DOIUrl":"https://doi.org/10.1590/1807-3107bor-2024.vol38.0024","url":null,"abstract":"<p><p>This study aimed to identify and characterize the antimicrobial susceptibility profile of bacteria found in primary endodontic infections in the teeth of patients treated at the Dental Clinic of the University of Ribeirão Preto, São Paulo, Brazil. From September to December 2019, samples were obtained from 21 patients with primary endodontic infections. The collections were carried out in triplicate using paper cones placed close to the total length of the root canal. Bacterial isolation was performed in Brain Heart Infusion agar, Blood agar, and other selective culture media cultured at 37°C for up to 48 h under aerobiosis and microaerophilic conditions. The bacterial species were identified using the Vitek 2 automated system. The disk diffusion method on agar Müeller-Hinton was used to assess antimicrobial susceptibility with the recommended antimicrobials for each identified bacterial species. A total of 49 antibiotics were evaluated. Fifteen of the 21 samples collected showed bacterial growth, and 17 bacterial isolates were found. There were 10 different bacterial species identified: Enterococcus faecalis (four isolates), Streptococcus mitis/oralis (three isolates), Streptococcus anginosus (three isolates) being the most common, followed by Staphylococcus epidermidis, Enterococcus faecium, Streptococcus constellatus, Streptococcus alactolyticus, Enterobacter cloacae, Klebsiella variicola, and Providencia rettgeri (one isolate of each species). The analysis demonstrated significant susceptibility to most of the tested antibiotics. However, some Enterococcus isolates resisted the antibiotic's erythromycin, ciprofloxacin, and tetracycline. A Staphylococcus epidermidis isolate was characterized as multidrug-resistant. Five Streptococcus isolates were non-susceptible to all antibiotics tested.</p>","PeriodicalId":9240,"journal":{"name":"Brazilian oral research","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140849556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-05eCollection Date: 2024-01-01DOI: 10.1590/1807-3107bor-2024.vol38.0025
Fernanda Vieira Heimlich, José Alcides Almeida de Arruda, Camila de Nazaré Alves de Oliveira Kato, Leni Verônica de Oliveira Silva, Leandro Napier Souza, Marcus Vinicius Lucas Ferreira, João de Jesus Viana Pinheiro, Tarcília Aparecida Silva, Lucas Guimarães Abreu, Ricardo Alves Mesquita
Treatment of oral vascular anomalies (OVA) has focused on minimally invasive techniques rather than radical surgery. We investigated the efficacy and safety of diode laser using the photocoagulation technique in the management of OVA. Forty-seven subjects with OVA were treated with forced dehydration with induced photocoagulation (FDIP) using diode laser (808 nm/4.5 W). This series consisted mostly of male (63.8%) and non-white (63.8%) patients with a mean age of 57.4 years. Varices (91.5%), venous malformations (6.4%), and hemangiomas (2.1%) with a mean size of 7.1 (±4.9) mm were the conditions treated. OVA presented as a nodular lesion (63.8%) involving mainly the lower lip (46.8%). Pulsed laser mode was used as standard and the number of applications varied from one to four sessions, with the majority requiring only one (83%) FDIP session. Kaplan-Meier analysis revealed that complete clinical healing can occur on the 15th day (n=9/29.5%), followed by the 20th (n=6/45.5%), and 30th (n=7/70.5%) days. Postoperative edema was observed in 31 (66%) patients, and recurrence of the lesion occurred in two (4.2%). Based on the data on complete clinical healing, minimal patient discomfort, and satisfactory esthetic results, we can confirm that FDIP by diode laser is a promising candidate for the safe and efficacious treatment of OVA.
口腔血管异常(OVA)的治疗侧重于微创技术,而非根治性手术。我们研究了二极管激光光凝技术治疗 OVA 的有效性和安全性。我们使用二极管激光(808 nm/4.5 W)对 47 例 OVA 患者进行了强迫脱水诱导光凝(FDIP)治疗。这一系列患者大多为男性(63.8%)和非白人(63.8%),平均年龄为 57.4 岁。接受治疗的病症包括静脉曲张(91.5%)、静脉畸形(6.4%)和血管瘤(2.1%),平均大小为 7.1 (±4.9) 毫米。OVA表现为结节性病变(63.8%),主要累及下唇(46.8%)。治疗以脉冲激光模式为标准,治疗次数从一次到四次不等,大多数患者(83%)只需一次FDIP治疗。Kaplan-Meier分析显示,临床完全愈合可发生在第15天(9/29.5%),其次是第20天(6/45.5%)和第30天(7/70.5%)。31例(66%)患者术后出现水肿,2例(4.2%)患者病灶复发。根据这些数据,我们可以确定二极管激光 FDIP 是一种安全有效的 OVA 治疗方法。
{"title":"Experience with 808-nm diode laser in the treatment of 47 cases of oral vascular anomalies.","authors":"Fernanda Vieira Heimlich, José Alcides Almeida de Arruda, Camila de Nazaré Alves de Oliveira Kato, Leni Verônica de Oliveira Silva, Leandro Napier Souza, Marcus Vinicius Lucas Ferreira, João de Jesus Viana Pinheiro, Tarcília Aparecida Silva, Lucas Guimarães Abreu, Ricardo Alves Mesquita","doi":"10.1590/1807-3107bor-2024.vol38.0025","DOIUrl":"10.1590/1807-3107bor-2024.vol38.0025","url":null,"abstract":"<p><p>Treatment of oral vascular anomalies (OVA) has focused on minimally invasive techniques rather than radical surgery. We investigated the efficacy and safety of diode laser using the photocoagulation technique in the management of OVA. Forty-seven subjects with OVA were treated with forced dehydration with induced photocoagulation (FDIP) using diode laser (808 nm/4.5 W). This series consisted mostly of male (63.8%) and non-white (63.8%) patients with a mean age of 57.4 years. Varices (91.5%), venous malformations (6.4%), and hemangiomas (2.1%) with a mean size of 7.1 (±4.9) mm were the conditions treated. OVA presented as a nodular lesion (63.8%) involving mainly the lower lip (46.8%). Pulsed laser mode was used as standard and the number of applications varied from one to four sessions, with the majority requiring only one (83%) FDIP session. Kaplan-Meier analysis revealed that complete clinical healing can occur on the 15th day (n=9/29.5%), followed by the 20th (n=6/45.5%), and 30th (n=7/70.5%) days. Postoperative edema was observed in 31 (66%) patients, and recurrence of the lesion occurred in two (4.2%). Based on the data on complete clinical healing, minimal patient discomfort, and satisfactory esthetic results, we can confirm that FDIP by diode laser is a promising candidate for the safe and efficacious treatment of OVA.</p>","PeriodicalId":9240,"journal":{"name":"Brazilian oral research","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140847162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-05eCollection Date: 2024-01-01DOI: 10.1590/1807-3107bor-2024.vol38.0023
Ana Luiza Cardoso Pires, Francine Dos Santos Costa, Otávio Pereira D'Ávila, Rodrigo Varella de Carvalho, Marcus Cristian Muniz Conde, Marcos Britto Correa, Flávio Fernando Demarco, Luiz Alexandre Chisini
The present study aimed to investigate the contextual inequalities of specialized public dental care (SPDC) in Brazil. The outcome was the trajectory of dental specialized production in municipalities with SPDC (from 2015 to 2017) obtained by group-based trajectory modeling. A Poisson regression model was used to analyze the factors associated with the high trajectory of SPDC production. The inequality indicators for SPDC production were the slope index and the concentration index according to contextual factors. The study included 954 SPDC units distributed across 893 municipalities. Among the municipalities evaluated, 62.9% had a low trajectory of SPDC. Large-sized municipalities had the highest production (IRR = 2.84, 95%CI: 1.94-4.14) and the southern region had the lowest production (IRR = 0.73, 95%CI: 0.58-0.92). Municipalities presenting a very high human development index (HDI) showed the greatest SPDC production (IRR = 3.34, 95%CI: 1.09-10.24), as well as municipalities with the highest tertile of schooling rate (IRR = 1.23, 95%CI: 1.00-1.50). The absolute inequality was 52.1 percentage points for the average monthly wage (p < 0.001), 61.0 percentage points for the HDI (p < 0.001), -22.1 for infant mortality rate (p <0.001), and 14.8 for the schooling rate (p = 0.012). Thus, there are contextual inequalities in the Brazilian SPDC. Higher scores for social indicators were associated with better SPDC performance.
{"title":"Contextual inequalities in specialized dental public health care in Brazil.","authors":"Ana Luiza Cardoso Pires, Francine Dos Santos Costa, Otávio Pereira D'Ávila, Rodrigo Varella de Carvalho, Marcus Cristian Muniz Conde, Marcos Britto Correa, Flávio Fernando Demarco, Luiz Alexandre Chisini","doi":"10.1590/1807-3107bor-2024.vol38.0023","DOIUrl":"https://doi.org/10.1590/1807-3107bor-2024.vol38.0023","url":null,"abstract":"<p><p>The present study aimed to investigate the contextual inequalities of specialized public dental care (SPDC) in Brazil. The outcome was the trajectory of dental specialized production in municipalities with SPDC (from 2015 to 2017) obtained by group-based trajectory modeling. A Poisson regression model was used to analyze the factors associated with the high trajectory of SPDC production. The inequality indicators for SPDC production were the slope index and the concentration index according to contextual factors. The study included 954 SPDC units distributed across 893 municipalities. Among the municipalities evaluated, 62.9% had a low trajectory of SPDC. Large-sized municipalities had the highest production (IRR = 2.84, 95%CI: 1.94-4.14) and the southern region had the lowest production (IRR = 0.73, 95%CI: 0.58-0.92). Municipalities presenting a very high human development index (HDI) showed the greatest SPDC production (IRR = 3.34, 95%CI: 1.09-10.24), as well as municipalities with the highest tertile of schooling rate (IRR = 1.23, 95%CI: 1.00-1.50). The absolute inequality was 52.1 percentage points for the average monthly wage (p < 0.001), 61.0 percentage points for the HDI (p < 0.001), -22.1 for infant mortality rate (p <0.001), and 14.8 for the schooling rate (p = 0.012). Thus, there are contextual inequalities in the Brazilian SPDC. Higher scores for social indicators were associated with better SPDC performance.</p>","PeriodicalId":9240,"journal":{"name":"Brazilian oral research","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140851721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-05eCollection Date: 2024-01-01DOI: 10.1590/1807-3107bor-2024.vol38.0026
Lucia Helena da Silva Ferreira Ancillotti, Mauro Henrique Nogueira Guimarães de Abreu, Angélica Maria Cupertino Lopes Marinho, Marcia Pereira Alves Dos Santos
This study validated the content of an instrument designed to assess the knowledge, involvement (attitudes) and management (practice) of dentists relative to sickle-cell disease (KAPD-SCD). The instrument consisted of five domains composed of a total of thirteen items: I. Dentist's self-assessment relative to sickle-cell disease; II. Dentist's knowledge of the repercussions of sickle-cell disease on the stomatognathic system; III. Dentist's knowledge of the complications of sickle-cell disease in the stomatognathic system; IV. Dentist's knowledge concerning the dental management of sickle-cell disease patients; and V. Dentist's involvement in an approach to sickle-cell disease. Twelve experts assigned scores to each item of the instrument. The criteria were clarity, understanding and appropriateness, leaving open fields for comments. Descriptive and content analyses of the data were made. Each expert analyzed 39 assessment units. The percentages considered for agreement were high (>80%), medium (70%-80%), or low (<70%), and each item was maintained or revised according to the percentage observed. There was high consensus in 74% of the assessment units (the corresponding items were maintained), medium consensus in 24% of them (the corresponding items were revised), and disagreement in 2% of them, namely as regards the "appropriateness" of item 5 ("Are there oral complications in sickle-cell disease?"), which was revised. The final version of the instrument had 16 items for different applications such as in the clinical care program, teaching program, or research program, with different cut-off scores for each application. In conclusion, the level of agreement among experts showed evidence of the content validity of the instrument.
本研究验证了旨在评估牙医对镰状细胞病的了解、参与(态度)和管理(实践)的工具(KAPD-SCD)的内容。该工具由五个领域共十三个项目组成:I. 牙医对镰状细胞病的自我评估;II.牙医对镰状细胞病对口腔系统影响的了解;III.牙科医生对镰状细胞病在口颌系统的并发症的了解; IV.牙科医生对镰状细胞病患者牙科治疗的了解;以及 V. 牙科医生参与镰状细胞病治疗的情况。12 位专家为该工具的每个项目打分。评分标准为清晰度、理解度和适当性,并留出了评论空间。对数据进行了描述性分析和内容分析。每位专家分析了 39 个评估单元。一致性的百分比分为高(>80%)、中(70%-80%)或低(>80%)。
{"title":"Validating evidence for the knowledge, management and involvement of dentists in a dental approach to sickle-cell disease.","authors":"Lucia Helena da Silva Ferreira Ancillotti, Mauro Henrique Nogueira Guimarães de Abreu, Angélica Maria Cupertino Lopes Marinho, Marcia Pereira Alves Dos Santos","doi":"10.1590/1807-3107bor-2024.vol38.0026","DOIUrl":"https://doi.org/10.1590/1807-3107bor-2024.vol38.0026","url":null,"abstract":"<p><p>This study validated the content of an instrument designed to assess the knowledge, involvement (attitudes) and management (practice) of dentists relative to sickle-cell disease (KAPD-SCD). The instrument consisted of five domains composed of a total of thirteen items: I. Dentist's self-assessment relative to sickle-cell disease; II. Dentist's knowledge of the repercussions of sickle-cell disease on the stomatognathic system; III. Dentist's knowledge of the complications of sickle-cell disease in the stomatognathic system; IV. Dentist's knowledge concerning the dental management of sickle-cell disease patients; and V. Dentist's involvement in an approach to sickle-cell disease. Twelve experts assigned scores to each item of the instrument. The criteria were clarity, understanding and appropriateness, leaving open fields for comments. Descriptive and content analyses of the data were made. Each expert analyzed 39 assessment units. The percentages considered for agreement were high (>80%), medium (70%-80%), or low (<70%), and each item was maintained or revised according to the percentage observed. There was high consensus in 74% of the assessment units (the corresponding items were maintained), medium consensus in 24% of them (the corresponding items were revised), and disagreement in 2% of them, namely as regards the \"appropriateness\" of item 5 (\"Are there oral complications in sickle-cell disease?\"), which was revised. The final version of the instrument had 16 items for different applications such as in the clinical care program, teaching program, or research program, with different cut-off scores for each application. In conclusion, the level of agreement among experts showed evidence of the content validity of the instrument.</p>","PeriodicalId":9240,"journal":{"name":"Brazilian oral research","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140853544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-11eCollection Date: 2024-01-01DOI: 10.1590/1807-3107bor-2024.vol38.0016
Vanessa de Carvalho Jovito, Jefferson Muniz de Lima, Marianne de Lucena Rangel, Brenna Louise Cavalcanti Gondim, Paula Lima Nogueira, Ana Claúdia Dantas de Medeiros, Marianna Vieira Sobral, Ricardo Dias de Castro, Lúcio Roberto Cançado Castellano
The pathogenic nature of infections caused by Candida spp. underscores the necessity for novel therapeutic agents. Extracts of Schinopsis brasilienses Engl are a promising source of agents with antifungal effects. This study aimed to assess the antifungal potential of the leaf extract of S. brasilienses. The antifungal activity was evaluated by determining the minimum inhibitory concentrations and fungicide concentrations (MIC and MFC). The antibiofilm potential was assessed by counting colony-forming units/mL. The study examined the inhibition kinetics of fungal growth and potential synergism between gallic acid or the extract and nystatin using the Checkerboard method. Cytotoxicity was evaluated through the MTT assay. The extract exhibited antifungal effect against all tested strains, with MIC and MFC ranging from 31.25-250 μg/mL. Gallic acid, the main isolated compound, displayed a MIC of 2000 μg/mL. The extract of S. brasilienses at 31.25 μg/mL inhibited the formation of biofilm by C. albicans and significantly reduced the mass of mature biofilm after 24 and 48 h (p < 0. 05). At a concentration of 125 μg/mL, the extract demonstrated significant inhibition of fungal growth after 6 hours. The combination of gallic acid or extract with nystatin did not exhibit synergistic or antagonistic effect. Furthermore, the extract did not induce cytotoxicity to a human cell line. The extract of S. brasiliensis demonstrates antifungal activity against Candida, generally exhibiting fungicidal action and capacity to inhibit biofilm formation as well as reduce mature biofilms. Additionally, the extract showed low cytotoxicity to human cells.
{"title":"Anticandida and antibiofilm activities of extract from Schinopsis brasiliensis Engl. against Candida spp.","authors":"Vanessa de Carvalho Jovito, Jefferson Muniz de Lima, Marianne de Lucena Rangel, Brenna Louise Cavalcanti Gondim, Paula Lima Nogueira, Ana Claúdia Dantas de Medeiros, Marianna Vieira Sobral, Ricardo Dias de Castro, Lúcio Roberto Cançado Castellano","doi":"10.1590/1807-3107bor-2024.vol38.0016","DOIUrl":"10.1590/1807-3107bor-2024.vol38.0016","url":null,"abstract":"<p><p>The pathogenic nature of infections caused by Candida spp. underscores the necessity for novel therapeutic agents. Extracts of Schinopsis brasilienses Engl are a promising source of agents with antifungal effects. This study aimed to assess the antifungal potential of the leaf extract of S. brasilienses. The antifungal activity was evaluated by determining the minimum inhibitory concentrations and fungicide concentrations (MIC and MFC). The antibiofilm potential was assessed by counting colony-forming units/mL. The study examined the inhibition kinetics of fungal growth and potential synergism between gallic acid or the extract and nystatin using the Checkerboard method. Cytotoxicity was evaluated through the MTT assay. The extract exhibited antifungal effect against all tested strains, with MIC and MFC ranging from 31.25-250 μg/mL. Gallic acid, the main isolated compound, displayed a MIC of 2000 μg/mL. The extract of S. brasilienses at 31.25 μg/mL inhibited the formation of biofilm by C. albicans and significantly reduced the mass of mature biofilm after 24 and 48 h (p < 0. 05). At a concentration of 125 μg/mL, the extract demonstrated significant inhibition of fungal growth after 6 hours. The combination of gallic acid or extract with nystatin did not exhibit synergistic or antagonistic effect. Furthermore, the extract did not induce cytotoxicity to a human cell line. The extract of S. brasiliensis demonstrates antifungal activity against Candida, generally exhibiting fungicidal action and capacity to inhibit biofilm formation as well as reduce mature biofilms. Additionally, the extract showed low cytotoxicity to human cells.</p>","PeriodicalId":9240,"journal":{"name":"Brazilian oral research","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140109230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-11eCollection Date: 2024-01-01DOI: 10.1590/1807-3107bor-2024.vol38.0021
Humberto Osvaldo Schwartz-Filho, Tauane Ramaldes Martins, Paulo Roberto Sano, Marcela Takemoto Araújo, Daniel Cheuk Hong Chan, Nathália Ramaldes Saldanha, Kátia de Pádua Silva, Talita Signoreti Graziano, William Cunha Brandt, Caio Vinícius Roman Torres, Karina Cogo-Müller
The present study aimed to evaluate the influence of titanium surface nanotopography on the initial bacterial adhesion process by in vivo and in vitro study models. Titanium disks were produced and characterized according to their surface topography: machined (Ti-M), microtopography (Ti-Micro), and nanotopography (Ti-Nano). For the in vivo study, 18 subjects wore oral acrylic splints containing 2 disks from each group for 24 h (n = 36). After this period, the disks were removed from the splints and evaluated by microbial culture method, scanning electron microscopy (SEM), and qPCR for quantification of Streptococcus oralis, Actinomyces naeslundii, Fusobacterium nucleatum, as well as total bacteria. For the in vitro study, adhesion tests were performed with the species S. oralis and A. naeslundii for 24 h. Data were compared by ANOVA, with Tukey's post-test. Regarding the in vivo study, both the total aerobic and total anaerobic bacteria counts were similar among groups (p > 0.05). In qPCR, there was no difference among groups of bacteria adhered to the disks (p > 0.05), except for A. naeslundii, which was found in lower proportions in the Ti-Nano group (p < 0.05). In the SEM analysis, the groups had a similar bacterial distribution, with a predominance of cocci and few bacilli. In the in vitro study, there was no difference in the adhesion profile for S. oralis and A. naeslundii after 24 h of biofilm formation (p > 0.05). Thus, we conclude that micro- and nanotopography do not affect bacterial adhesion, considering an initial period of biofilm formation.
{"title":"Nanotopography and oral bacterial adhesion on titanium surfaces: in vitro and in vivo studies.","authors":"Humberto Osvaldo Schwartz-Filho, Tauane Ramaldes Martins, Paulo Roberto Sano, Marcela Takemoto Araújo, Daniel Cheuk Hong Chan, Nathália Ramaldes Saldanha, Kátia de Pádua Silva, Talita Signoreti Graziano, William Cunha Brandt, Caio Vinícius Roman Torres, Karina Cogo-Müller","doi":"10.1590/1807-3107bor-2024.vol38.0021","DOIUrl":"10.1590/1807-3107bor-2024.vol38.0021","url":null,"abstract":"<p><p>The present study aimed to evaluate the influence of titanium surface nanotopography on the initial bacterial adhesion process by in vivo and in vitro study models. Titanium disks were produced and characterized according to their surface topography: machined (Ti-M), microtopography (Ti-Micro), and nanotopography (Ti-Nano). For the in vivo study, 18 subjects wore oral acrylic splints containing 2 disks from each group for 24 h (n = 36). After this period, the disks were removed from the splints and evaluated by microbial culture method, scanning electron microscopy (SEM), and qPCR for quantification of Streptococcus oralis, Actinomyces naeslundii, Fusobacterium nucleatum, as well as total bacteria. For the in vitro study, adhesion tests were performed with the species S. oralis and A. naeslundii for 24 h. Data were compared by ANOVA, with Tukey's post-test. Regarding the in vivo study, both the total aerobic and total anaerobic bacteria counts were similar among groups (p > 0.05). In qPCR, there was no difference among groups of bacteria adhered to the disks (p > 0.05), except for A. naeslundii, which was found in lower proportions in the Ti-Nano group (p < 0.05). In the SEM analysis, the groups had a similar bacterial distribution, with a predominance of cocci and few bacilli. In the in vitro study, there was no difference in the adhesion profile for S. oralis and A. naeslundii after 24 h of biofilm formation (p > 0.05). Thus, we conclude that micro- and nanotopography do not affect bacterial adhesion, considering an initial period of biofilm formation.</p>","PeriodicalId":9240,"journal":{"name":"Brazilian oral research","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140109234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-11eCollection Date: 2024-01-01DOI: 10.1590/1807-3107bor-2024.vol38.0017
Layza Rossatto Oppitz, Ana Carolina Mastriani Arantes, Roberto Ramos Garanhani, Carlos Alberto Costa, Cristiano Miranda de Araujo, Orlando Motohiro Tanaka, Patricia Kern di Scala Andreis, Claudia Schappo, Sérgio Aparecido Ignácio, Aline Cristina Batista Rodrigues Johann, Rodrigo Nunes Rached, Elisa Souza Camargo
Occlusal stabilization splints are the most common treatment for controlling the deleterious effects of sleep bruxism. This study aimed to evaluate the effectiveness of a low-cost, mixed occlusal splint (MOS) compared to that of a rigid splint. A randomized clinical trial was performed on 43 adults of both sexes with possible sleep bruxism and satisfactory dental conditions. They were divided into rigid occlusal splint (ROS) (n = 23) and MOS (n = 20) groups. Masticatory muscle and temporomandibular joint (TMJ) pain intensity (visual analog scale), quality of life (WHOQOL-BREF), indentations in the oral mucosa, anxiety, and depression (HADS), number of days of splint use, and splint wear were evaluated. All variables were evaluated at baseline (T0), 6 months (T6), and 12 months (T12) after splint installation (T0), and splint wear was evaluated at T6 and T12. Student's t-test, Mann-Whitney U test, non-parametric Friedman's analysis of variance for paired samples and pairwise multiple comparisons, Pearson's chi-square test, two-proportion z-test, non-parametric McNemar's and Cochran's Q, and Wilcoxon tests were used (p < 0.05). In both groups, there was a decrease in TMJ pain and pain intensity over time and improvements in the quality of life scores. At T6, there was a higher rate of splint wear in the MOS group than in the ROS group (p = 0.023). The MOS showed a higher rate of wear than the rigid splint but had similar results for the other variables. Therefore, the use of a mixed splint appears to be effective in controlling the signs and symptoms of sleep bruxism.
咬合稳定夹板是控制睡眠磨牙症有害影响的最常见治疗方法。本研究旨在评估一种低成本的混合咬合夹板(MOS)与硬质夹板相比的效果。研究人员对 43 名可能患有睡眠磨牙症且牙齿状况良好的成年男女进行了随机临床试验。他们被分为刚性咬合夹板(ROS)组(23 人)和 MOS 组(20 人)。对咀嚼肌和颞下颌关节(TMJ)疼痛强度(视觉模拟量表)、生活质量(WHOQOL-BREF)、口腔粘膜压痕、焦虑和抑郁(HADS)、夹板使用天数以及夹板磨损情况进行了评估。所有变量均在夹板安装(T0)后的基线(T0)、6 个月(T6)和 12 个月(T12)时进行评估,夹板磨损情况在 T6 和 T12 时进行评估。采用了学生 t 检验、曼-惠特尼 U 检验、非参数弗里德曼配对样本方差分析和配对多重比较、皮尔逊卡方检验、两比例 z 检验、非参数麦克尼玛 Q 检验和科克兰 Q 检验以及 Wilcoxon 检验(P < 0.05)。随着时间的推移,两组患者的颞下颌关节疼痛和疼痛强度都有所减轻,生活质量评分也有所提高。在 T6 期,MOS 组的夹板磨损率高于 ROS 组(p = 0.023)。MOS夹板的磨损率高于硬夹板,但其他变量的结果相似。因此,使用混合夹板似乎可以有效控制睡眠磨牙症的症状和体征。
{"title":"Efficiency of mixed and rigid occlusal stabilization splints: Randomized clinical trial.","authors":"Layza Rossatto Oppitz, Ana Carolina Mastriani Arantes, Roberto Ramos Garanhani, Carlos Alberto Costa, Cristiano Miranda de Araujo, Orlando Motohiro Tanaka, Patricia Kern di Scala Andreis, Claudia Schappo, Sérgio Aparecido Ignácio, Aline Cristina Batista Rodrigues Johann, Rodrigo Nunes Rached, Elisa Souza Camargo","doi":"10.1590/1807-3107bor-2024.vol38.0017","DOIUrl":"10.1590/1807-3107bor-2024.vol38.0017","url":null,"abstract":"<p><p>Occlusal stabilization splints are the most common treatment for controlling the deleterious effects of sleep bruxism. This study aimed to evaluate the effectiveness of a low-cost, mixed occlusal splint (MOS) compared to that of a rigid splint. A randomized clinical trial was performed on 43 adults of both sexes with possible sleep bruxism and satisfactory dental conditions. They were divided into rigid occlusal splint (ROS) (n = 23) and MOS (n = 20) groups. Masticatory muscle and temporomandibular joint (TMJ) pain intensity (visual analog scale), quality of life (WHOQOL-BREF), indentations in the oral mucosa, anxiety, and depression (HADS), number of days of splint use, and splint wear were evaluated. All variables were evaluated at baseline (T0), 6 months (T6), and 12 months (T12) after splint installation (T0), and splint wear was evaluated at T6 and T12. Student's t-test, Mann-Whitney U test, non-parametric Friedman's analysis of variance for paired samples and pairwise multiple comparisons, Pearson's chi-square test, two-proportion z-test, non-parametric McNemar's and Cochran's Q, and Wilcoxon tests were used (p < 0.05). In both groups, there was a decrease in TMJ pain and pain intensity over time and improvements in the quality of life scores. At T6, there was a higher rate of splint wear in the MOS group than in the ROS group (p = 0.023). The MOS showed a higher rate of wear than the rigid splint but had similar results for the other variables. Therefore, the use of a mixed splint appears to be effective in controlling the signs and symptoms of sleep bruxism.</p>","PeriodicalId":9240,"journal":{"name":"Brazilian oral research","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140109232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-11eCollection Date: 2024-01-01DOI: 10.1590/1807-3107bor-2024.vol38.0018
Mariana Saturnino de Noronha, Karolina Skarlet Silva Viana, Maria Cássia Ferreira de Aguiar, Cristiane Helena Squarize, Mauro Henrique Nogueira Guimarães de Abreu, Elismauro Francisco Mendonça, Vanessa de Fátima Bernardes
The aim of this study was to evaluate the expression of the EZH2 protein and describe the clinical and microscopic characteristics of adenoid cystic carcinoma (ACC) and pleomorphic adenoma (PA). The study included 16 ACC cases and 12 PA. All ACC and PA cases were positive for EZH2 and the ACC samples showed significantly higher EZH2 expression. The clinical and microscopic covariates were described in relation to EZH2 staining in ACC samples. The highest mean values of EZH2 were observed in cases with local metastasis, recurrence, perineural invasion, and predominantly cribriform growth pattern without solid areas. EZH2 is a potential marker of malignancy.
{"title":"EZH2 immunoexpression in pleomorphic adenoma and adenoid cystic carcinoma and clinicopathological features.","authors":"Mariana Saturnino de Noronha, Karolina Skarlet Silva Viana, Maria Cássia Ferreira de Aguiar, Cristiane Helena Squarize, Mauro Henrique Nogueira Guimarães de Abreu, Elismauro Francisco Mendonça, Vanessa de Fátima Bernardes","doi":"10.1590/1807-3107bor-2024.vol38.0018","DOIUrl":"10.1590/1807-3107bor-2024.vol38.0018","url":null,"abstract":"<p><p>The aim of this study was to evaluate the expression of the EZH2 protein and describe the clinical and microscopic characteristics of adenoid cystic carcinoma (ACC) and pleomorphic adenoma (PA). The study included 16 ACC cases and 12 PA. All ACC and PA cases were positive for EZH2 and the ACC samples showed significantly higher EZH2 expression. The clinical and microscopic covariates were described in relation to EZH2 staining in ACC samples. The highest mean values of EZH2 were observed in cases with local metastasis, recurrence, perineural invasion, and predominantly cribriform growth pattern without solid areas. EZH2 is a potential marker of malignancy.</p>","PeriodicalId":9240,"journal":{"name":"Brazilian oral research","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140109233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-11eCollection Date: 2024-01-01DOI: 10.1590/1807-3107bor-2024.vol38.0019
Ana Paula Milagres Alfenas Silva, Jessica Klockner Knorst, Julia Rodrigues Andrade, Rafaela Silveira Pinto, Renata Castro Martins, Amália Moreno, Fabiana Vargas-Ferreira
{"title":"Contextual and individual determinants of oral health-related quality of life among adolescents.","authors":"Ana Paula Milagres Alfenas Silva, Jessica Klockner Knorst, Julia Rodrigues Andrade, Rafaela Silveira Pinto, Renata Castro Martins, Amália Moreno, Fabiana Vargas-Ferreira","doi":"10.1590/1807-3107bor-2024.vol38.0019","DOIUrl":"10.1590/1807-3107bor-2024.vol38.0019","url":null,"abstract":"","PeriodicalId":9240,"journal":{"name":"Brazilian oral research","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140109231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}