Brazilian oral research最新文献

To evaluate the pH, solubility and intratubular disinfection of the bioceramic intracanal drug Bio-C® Temp (BCT), calcium hydroxide-based paste Calen® (CAL) and their associations in different proportions: BCT 75% + CAL 25%; BCT 50% + CAL 50%; BCT 25% + CAL 75%. Polyethylene tubes containing the medication, were used. The pH was evaluated after 1, 3, 7, 14 and 21 days and solubility after 14 days. Bovine dentin tubes were contaminated with Enterococcus faecalis to assess intratubular disinfection by using confocal laser scanning microscopy and use of Live/Dead BacLight Bacterial stain. Data were submitted to statistical tests of normality, then ANOVA and Tukey (α = 0.05). BCT showed a lower pH after 3 and 14 days (p < 0.05). CAL had the highest pH at all time intervals (p > 0.05). CAL and associations with BCT showed greater weight loss (p < 0.05). BCT showed intratubular disinfection similar to that of BCT/CAL 25% (p > 0.05). CAL and BCT/CAL at 50% and 75% were similar and had the highest intratubular disinfection values (p > 0.05). Combinations of Bio-C® Temp with 50% or more calcium hydroxide paste provide higher alkalinization, solubility and intratubular disinfection values of the bioceramic medication, favoring its potential for clinical application.

Oral health literacy (OHL) may play a crucial role for managing traumatic dental injuries. In this pre-post study, the effect of the different dimensions of OHL on the efficacy of using an information leaflet (IL for managing permanent tooth avulsion ) was assessed in elementary schoolteachers. Functional OHL was measured using the Brazilian version of the Rapid Estimate of Adult Literacy in Dentistry (BREALD-30), while interactive OHL was assessed with the Oral Health Literacy Adult Questionnaire (BOHL-AQ). Knowledge scores (KS) on avulsion management were collected at three intervals: before reading the IL (baseline), immediately post-intervention (Phase I), and 60 days post-intervention (Phase II). The effect on OHL was assessed by comparing KS means between Phase I/Baseline (acquisition) and Phases II/I (retention). Participants with high OHL/BOHL had a greater difference in mean KS values between Phase I/baseline, while those with high OHL/BREALD-30 showed a smaller difference between phases II/I. Different dimensions of OHL impacted the effectiveness of an educational intervention on managing permanent tooth avulsion with use of a leaflet.

The aim of this study was to assess the biosafety practices adopted during the COVID-19 pandemic that persisted in post-pandemic orthodontic clinical routines. In this cross-sectional study, 722 Brazilian orthodontists who were in the early phase of the pandemic, and 203 from the later phase, respectively, answered a 45-item questionnaire addressing basic personal information, use of personal protective equipment, biosafety protocols, and COVID-19 incidence. Descriptive statistics and chi-square tests were used to analyze the data. Most participants were infected with the coronavirus (from 10.1% to 65%). While 52.4% perceived providing care as very risky during the pandemic, this perception shifted to decreased to a moderate level of risk in the late phase of the pandemic (40.4%). The use of protective face masks/face shields decreased from 51.7% to 25.1%, as well as the use of disposable coats (from 77.1% to 45.8%). Many orthodontists discontinued the use of PFF2/N95 masks and reverted to wearing surgical masks again. Most orthodontists disinfected orthodontic bands, and photographic retractors through manual washing and autoclaving. Most participants preferred to clean their orthodontic pliers with 70% alcohol. A decline in orthodontists' concern about biosafety during clinical appointments was observed in the late phase of the pandemic. Moreover, the need for greater specific care still persists, especially regarding the proper use of disposable coats, face shields, and surgical masks.

Metrics derived from online social platforms indicate current interest in a specific topic. This study aimed to analyze the characteristics and social interest in scientific publications on bruxism using an altmetric analysis. A search was conducted in August 2024 in the Dimensions database. The following data were extracted: altmetric attention score (AAS), citations, year, language, access type, study design, topic (general objective and age group), journal, country, institution, and authors. VOSviewer was used to generate collaborative networks, whereas Google Trends was consulted to assess public interest in bruxism-related research. A Spearman correlation analysis was performed to establish the relationship between AAS and citations. A total of 196 studies published between 1992 and 2024 were included. The most widely accessed study had an AAS of 393. Significant interest in bruxism was observed among Mendeley, news outlets, and X users. Most studies were observational (n = 99), addressing the etiologic factors of bruxism (n = 75) and focusing primarily on the adult population (n = 148). The Journal of Oral Rehabilitation stood out as the most relevant journal (n = 36). Most articles were published in Brazil (n = 40), and Lobbezoo was the most frequent author (n = 27). VOSviewer revealed significant collaborations among authors. Most studies were not openly accessible (n = 105). A very weak positive correlation (r = 0.042) was observed between AAS and the number of citations. This study highlighted a social interest in scientific publications on bruxism, particularly those addressing etiologic factors.

The objective of this study was to develop a bioabsorbable membrane composed of poly(butylene adipate-co-terephthalate) (PBAT) with alkyl trimethyl ammonium bromide (ATAB) to provide antimicrobial properties. Membranes were manufactured via the solvent casting technique using chloroform solutions containing PBAT and varying concentrations of ATAB (1, 2.5, and 5% wt), with ATAB-free membranes as a control. The characterization of the membranes included assessments of contact angle, surface free energy, and degradation in distilled water over periods of one week, one month, and three months. Mechanical properties were evaluated via tensile strength, and changes in water pH were monitored from 24 hours to three months post-immersion. Cytotoxicity was assessed using gingival fibroblasts and pre-osteoblasts via the SRB assay. Antimicrobial activity was tested against Staphylococcus aureus. ATAB inclusion reduced water contact angle and increased surface free energy compared to controls (p < 0.001). The addition of 5% wt ATAB decreased the tensile strength of PBAT membranes. At one month, a reduced mass was observed for the 2.5% wt ATAB membrane. The specimens' mass was reduced for all groups after three months of immersion in water in comparison to the initial measurement, while a reduction in thickness was found in all time points, without the influence of ATAB. ATAB incorporation reduced cell viability. Antimicrobial efficacy, resulting in a > 3 log10 bacterial reduction, was observed for S. aureus at the 5% wt concentration. The addition of 2.5% wt ATAB to PBAT membranes may be a suitable strategy to generate barrier membranes with an antibacterial effect while maintaining acceptable mechanical and surface properties.

In this randomized controlled clinical study, the effectiveness of two different modes of extending oral hygiene - either gargling or using an oral spray - on the self-reported incidence of upper respiratory symptoms were evaluated, to help with the prevention of respiratory symptoms. The study was conducted in Porto Alegre, Brazil. A total of 205 individuals were enrolled and assigned to one of three groups: Control Group: brushed twice daily for two minutes with a commercially available fluoride toothpaste; Gargling Group: brushed twice daily for two minutes with a commercially available fluoride toothpaste and then gargled with a mouthwash containing 0.075% CPC (cetylpiridinium chloride); and Oral Spray Group: brushed twice daily for two minutes with a commercially available fluoride toothpaste and instructed to use an oral spray containing 0.075% CPC (3 pumps of spray applied directly into their mouth) twice daily. All subjects were asked to complete the WURSS-21 Daily Symptom Report via a diary for the 3 months duration of the study. Based on analysis of the diaries, the use of regular oral hygiene with brushing, in addition to a type that extended to the entire oral cavity, reduced the incidence and severity of upper respiratory symptoms throughout the duration of the study. Data were statistically analyzed using ANOVA (α = 0.05). Individuals using either mouthwash or spray reported approximately 20% fewer days with respiratory symptoms compared with the control group. Regular oral hygiene that extended to the whole oral cavity with either a mouthwash or an oral spray may help to reduce the incidence of upper respiratory symptoms.

This study evaluated the cytotoxicity, biocompatibility, and bioactivity potential of bioglass solutions (F18 and F18 with cobalt; F18-Co) compared to Ca(OH)2 solution, to determine their suitability for use in vital pulp therapy. F18 bioglass was prepared, with a part being doped with cobalt. The solutions were prepared at a 1:10 powder-to-water ratio. L929 fibroblasts viability was assessed (MTT assay; 24 and 48-h). Tubes containing fibrin sponges embedded with either the solutions or saline (control) were prepared (16 tubes/group) and immediately implanted into 16 rats (4 tubes/rat). At 7 and 30 days, the euthanized rat specimens were analyzed for inflammation and bioactivity. The ANOVA with Tukey's test, or Kruskal-Wallis with Dunn's test was performed (p < 0.05). Undiluted, 1:2, and 1:4 diluted solutions reduced cell viability at 24-h (p < 0.05). The 1:8 and 1:16 dilutions of F18 and F18-Co exhibited cell viability similar to that of the control (p > 0.05), whereas Ca(OH)2 was cytotoxic (p < 0.05). At 48-h, F18 dilutions (undiluted, 1:2, and 1:4) exhibited similar results to the control (p > 0.05). F18-Co at 1:8 and 1:16 dilutions increased cell viability compared to Ca(OH)2 (p < 0.05) and were similar to the control (p > 0.05). On day 7, moderate-to-severe inflammation (p > 0.05) and thick fibrous capsule were observed. On day 30, mild inflammation was observed in the control and F18-Co groups, moderate inflammation in F18 (p < 0.05), and mild inflammation in Ca(OH)2 (p > 0.05). The fibrous capsule was thin. None of the materials exhibited positive structure in von Kossa and polarized light analysis. The F18 and F18-Co solutions are cyto- and biocompatible; however, no bioactivity was observed.

The aim of this study was to assess the impact of the apical patency technique on apical transportation and centering ability of a controlled memory (CM) instrument in the apical region using cone-beam computed tomography (CBCT). Sixty distobuccal canals of extracted maxillary molars were assigned to three groups (n = 20) based on the patency length achieved using the Easy ProDesign Logic (EPL) 25.01 file: Group A - 1 mm beyond the apical foramen; Group B - at the apical foramen; and Group C - 1 mm short of the apical foramen (no patency). Each group was then subdivided into two subgroups (n = 10) according to the working length used for root canal preparation with the EPL 25.05 file: A1, B1, C1-I, and C1-II - 1 mm short of the apical foramen and A2, B2 - at the apical foramen. CBCT images were acquired at three time points: pre-patency, post-patency, and post-instrumentation. The scanned images were analyzed using the E-VOL DX software. No statistically significant difference in apical transportation was found between the groups after patency or after instrumentation (p < 0.05), irrespective of the measurement levels (0.5, 1, and 2 mm short of the apical foramen). A significant difference in the centering ability of the patency instrument was observed only at 2 mm short of the foramen (p < 0.05). The B2 group exhibited a higher centering ability, with a statistically significant difference compared to the A2 group (p < 0.05), observed only at 0.5 mm short of the foramen. In conclusion, maintenance of apical patency using the EPL instrument had no influence on apical transportation; however, it may slightly affect the centering ability of the root canal.

This study compared the outcomes of two endodontic instrumentation protocols (rotary or reciprocating files), using artificial primary teeth and micro-computed tomography. Twenty-four artificial primary molars were equally distributed into two groups of 12, according to the type of instrumentation - rotary files (Sequence Baby NiTi Files©) or reciprocating files (X1-Blue File NiTi files©). The following parameters were evaluated: root canal and dentin volumes, canal transportation and centering ability, risk of root perforation, and time of instrumentation. Statistically significant differences between the two instrumentation protocols considering root canal transportation and centering ability were identified. There was no difference in dentin thickness, fractures, and cracks comparing preoperative and postoperative time points for both endodontic files. Although the time of instrumentation was shorter for reciprocating files, rotary files promoted smaller root canal enlargement. Instrumentation with reciprocating and rotary files proved generally safe for pulpectomy in primary molars, promoting a negligible reduction in dentin volume, canal transportation, and centering ability, thereby preserving dentin thickness and lowering the risk of fractures.

Although social capital can significantly impact oral health-related quality of life (OHRQoL), evidence of this association remains limited during pregnancy. We assessed the association between individual social capital and OHRQoL among pregnant women in public health services. This cross-sectional study assessed a representative sample of pregnant women grouped into 25 Brazilian public health units in 2022. Sociodemographic, individual social capital (social networks and social support), and health behaviour characteristics were collected through a questionnaire. Participants were also clinically examined for gingivitis and dental caries experience. The outcome OHRQoL was assessed using the Oral Health Impact Profile (OHIP-14) questionnaire. Multilevel Poisson regression models with a hierarchical approach were used to assess the associations between social capital and overall OHIP-14 score. The results are presented as rate ratio (RR) and 95% confidence interval (95%CI). A total of 520 pregnant women were evaluated. Pregnant women with lower social support had higher overall OHIP-14 scores (RR: 1.21, 95%CI: 1.10-1.34). However, social networks were not associated with OHRQoL (p > 0.05). High number of children, lower household income and schooling factors, alcohol consumption before pregnancy, visiting a dentist for toothache, gingivitis, and dental caries experience were also associated with higher overall OHIP-14 scores (p < 0.05). Lower social support was associated with worse OHRQoL among pregnant women. These finding suggest that quality of personal resources was of greater relevance than social networks during pregnancy.