Brazilian oral research最新文献

This study evaluated the cytotoxicity, biocompatibility, and bioactivity potential of bioglass solutions (F18 and F18 with cobalt; F18-Co) compared to Ca(OH)2 solution, to determine their suitability for use in vital pulp therapy. F18 bioglass was prepared, with a part being doped with cobalt. The solutions were prepared at a 1:10 powder-to-water ratio. L929 fibroblasts viability was assessed (MTT assay; 24 and 48-h). Tubes containing fibrin sponges embedded with either the solutions or saline (control) were prepared (16 tubes/group) and immediately implanted into 16 rats (4 tubes/rat). At 7 and 30 days, the euthanized rat specimens were analyzed for inflammation and bioactivity. The ANOVA with Tukey's test, or Kruskal-Wallis with Dunn's test was performed (p < 0.05). Undiluted, 1:2, and 1:4 diluted solutions reduced cell viability at 24-h (p < 0.05). The 1:8 and 1:16 dilutions of F18 and F18-Co exhibited cell viability similar to that of the control (p > 0.05), whereas Ca(OH)2 was cytotoxic (p < 0.05). At 48-h, F18 dilutions (undiluted, 1:2, and 1:4) exhibited similar results to the control (p > 0.05). F18-Co at 1:8 and 1:16 dilutions increased cell viability compared to Ca(OH)2 (p < 0.05) and were similar to the control (p > 0.05). On day 7, moderate-to-severe inflammation (p > 0.05) and thick fibrous capsule were observed. On day 30, mild inflammation was observed in the control and F18-Co groups, moderate inflammation in F18 (p < 0.05), and mild inflammation in Ca(OH)2 (p > 0.05). The fibrous capsule was thin. None of the materials exhibited positive structure in von Kossa and polarized light analysis. The F18 and F18-Co solutions are cyto- and biocompatible; however, no bioactivity was observed.

In this randomized controlled clinical study, the effectiveness of two different modes of extending oral hygiene - either gargling or using an oral spray - on the self-reported incidence of upper respiratory symptoms were evaluated, to help with the prevention of respiratory symptoms. The study was conducted in Porto Alegre, Brazil. A total of 205 individuals were enrolled and assigned to one of three groups: Control Group: brushed twice daily for two minutes with a commercially available fluoride toothpaste; Gargling Group: brushed twice daily for two minutes with a commercially available fluoride toothpaste and then gargled with a mouthwash containing 0.075% CPC (cetylpiridinium chloride); and Oral Spray Group: brushed twice daily for two minutes with a commercially available fluoride toothpaste and instructed to use an oral spray containing 0.075% CPC (3 pumps of spray applied directly into their mouth) twice daily. All subjects were asked to complete the WURSS-21 Daily Symptom Report via a diary for the 3 months duration of the study. Based on analysis of the diaries, the use of regular oral hygiene with brushing, in addition to a type that extended to the entire oral cavity, reduced the incidence and severity of upper respiratory symptoms throughout the duration of the study. Data were statistically analyzed using ANOVA (α = 0.05). Individuals using either mouthwash or spray reported approximately 20% fewer days with respiratory symptoms compared with the control group. Regular oral hygiene that extended to the whole oral cavity with either a mouthwash or an oral spray may help to reduce the incidence of upper respiratory symptoms.

The aim of this study was to assess the impact of the apical patency technique on apical transportation and centering ability of a controlled memory (CM) instrument in the apical region using cone-beam computed tomography (CBCT). Sixty distobuccal canals of extracted maxillary molars were assigned to three groups (n = 20) based on the patency length achieved using the Easy ProDesign Logic (EPL) 25.01 file: Group A - 1 mm beyond the apical foramen; Group B - at the apical foramen; and Group C - 1 mm short of the apical foramen (no patency). Each group was then subdivided into two subgroups (n = 10) according to the working length used for root canal preparation with the EPL 25.05 file: A1, B1, C1-I, and C1-II - 1 mm short of the apical foramen and A2, B2 - at the apical foramen. CBCT images were acquired at three time points: pre-patency, post-patency, and post-instrumentation. The scanned images were analyzed using the E-VOL DX software. No statistically significant difference in apical transportation was found between the groups after patency or after instrumentation (p < 0.05), irrespective of the measurement levels (0.5, 1, and 2 mm short of the apical foramen). A significant difference in the centering ability of the patency instrument was observed only at 2 mm short of the foramen (p < 0.05). The B2 group exhibited a higher centering ability, with a statistically significant difference compared to the A2 group (p < 0.05), observed only at 0.5 mm short of the foramen. In conclusion, maintenance of apical patency using the EPL instrument had no influence on apical transportation; however, it may slightly affect the centering ability of the root canal.

This study compared the outcomes of two endodontic instrumentation protocols (rotary or reciprocating files), using artificial primary teeth and micro-computed tomography. Twenty-four artificial primary molars were equally distributed into two groups of 12, according to the type of instrumentation - rotary files (Sequence Baby NiTi Files©) or reciprocating files (X1-Blue File NiTi files©). The following parameters were evaluated: root canal and dentin volumes, canal transportation and centering ability, risk of root perforation, and time of instrumentation. Statistically significant differences between the two instrumentation protocols considering root canal transportation and centering ability were identified. There was no difference in dentin thickness, fractures, and cracks comparing preoperative and postoperative time points for both endodontic files. Although the time of instrumentation was shorter for reciprocating files, rotary files promoted smaller root canal enlargement. Instrumentation with reciprocating and rotary files proved generally safe for pulpectomy in primary molars, promoting a negligible reduction in dentin volume, canal transportation, and centering ability, thereby preserving dentin thickness and lowering the risk of fractures.

Although social capital can significantly impact oral health-related quality of life (OHRQoL), evidence of this association remains limited during pregnancy. We assessed the association between individual social capital and OHRQoL among pregnant women in public health services. This cross-sectional study assessed a representative sample of pregnant women grouped into 25 Brazilian public health units in 2022. Sociodemographic, individual social capital (social networks and social support), and health behaviour characteristics were collected through a questionnaire. Participants were also clinically examined for gingivitis and dental caries experience. The outcome OHRQoL was assessed using the Oral Health Impact Profile (OHIP-14) questionnaire. Multilevel Poisson regression models with a hierarchical approach were used to assess the associations between social capital and overall OHIP-14 score. The results are presented as rate ratio (RR) and 95% confidence interval (95%CI). A total of 520 pregnant women were evaluated. Pregnant women with lower social support had higher overall OHIP-14 scores (RR: 1.21, 95%CI: 1.10-1.34). However, social networks were not associated with OHRQoL (p > 0.05). High number of children, lower household income and schooling factors, alcohol consumption before pregnancy, visiting a dentist for toothache, gingivitis, and dental caries experience were also associated with higher overall OHIP-14 scores (p < 0.05). Lower social support was associated with worse OHRQoL among pregnant women. These finding suggest that quality of personal resources was of greater relevance than social networks during pregnancy.

This study aimed to evaluate the influence of pacifier use, bottle feeding, and finger/thumb sucking on the occurrence of otitis media in children. Searches were conducted in grey literature and six databases: Web of Science, Cochrane Library, LILACS, Scopus, MEDLINE/PubMed, and EMBASE, from inception through May 2025. Observational studies investigating the association between harmful sucking habits and otitis media in newborns, infants, preschoolers, and older children were included. Risk of bias was assessed using the Joanna Briggs Institute tool. Meta-analysis results were reported as odds ratios (ORs) with 95% confidence intervals (CIs), and the certainty of evidence was also evaluated. A total of 36 studies were included, most of which were cohort studies (n = 14), with sample sizes ranging from 46 to 35,613. Eleven studies were incorporated into meta-analyses. Children who used a pacifier were 1.17 times more likely to develop otitis media (95%CI: 1.00-1.33) and 1.54 times more likely to develop acute otitis media (95%CI: 1.01-2.36) compared to those who did not use a pacifier. No significant association was found between bottle feeding and otitis media (OR = 0.83; 95%CI: 0.59-1.17). Most studies did not report a significant association between finger or thumb sucking and otitis media. The certainty of evidence was rated as very low. These findings suggest that pacifier use may increase the likelihood of developing otitis media, particularly acute otitis media, with potentially relevant implications.

Guided bone regeneration (GBR) is a critical strategy for repairing large bone defects. This study aimed to assess the osteopromotive potential of a porcine cross-linked collagen membrane in critical calvaria-defects in rats. Seventy-two rats were divided into three groups: blood clot (CG - negative control), Bio-Gide® membrane (BG - positive control), and Ossix Plus® membrane (OSX - test). The defects were covered according to each group, and the experimental times were 7, 15, 30, and 60 days postoperatively. The collected samples were evaluated by histometric analysis, inflammatory profile, immunohistochemistry, and micro-computed tomography (micro-CT). At 7 days, no significant differences in bone neoformation were observed. At 15 days, the OSX group showed increased new bone formation compared to CG (p = 0.031). At 30 days, the BG group exhibited the most significant increase compared to both OSX (p < 0.001) and CG (p < 0.001). At 60 days, OSX demonstrated the highest osteopromotive potential, significantly outperforming CG (p < 0.001) and BG (p < 0.001). MicroCT analysis revealed that the OSX group had a bone volume (BV) of 17.33 ± 1.74 mm3, significantly higher than BG (8.06 ± 1.26 mm3, p < 0.05). The cross-linked collagen membrane was biologically more favorable for bone regeneration being a promising option for GBR procedures.

This study aimed to evaluate the impact of the blooming artifact reduction (BAR) algorithm on the measurement of various sealing materials (Biodentine, MTA ProRoot, MTAFlow, and amalgam) for furcal perforation repair using cone-beam computed tomography (CBCT). A total of 105 extracted human mandibular first and second molars were prepared and root-filled. Furcal perforations were created, and the specimens were randomly organized into five groups based on the sealing material used: (1) Biodentine, (2) MTA ProRoot, (3) MTAFlow, (4) Amalgam, and (5) Control (no sealing material). After setting, the specimens were embedded in wax, and the crowns were sectioned to measure the diameter of the sealing materials using a digital micrometer. CBCT scans were acquired using the Prexion 3D Elite® CT scanner. The dimensional measurements of sealing materials on the CBCT images was measured using DICOM files and the e-Vol DX software measurement tool, configured to thousandths of a millimeter. BAR algorithms were applied as follows: BAR 3 for the control group, Biodentine, and MTAFlow; BAR 2 for MTA ProRoot; and BAR 1 for amalgam. Statistical analysis was performed using ANOVA and Tukey's test with a significance level of 5%. No significant differences were observed between the diameters of sealing materials measured using the micrometer and those obtained from CBCT images processed with BAR algorithms (Biodentine, MTA ProRoot, MTAFlow, and amalgam). The BAR algorithm in the e-Vol DX software preserved the dimensional accuracy of CBCT images for the tested sealing materials.

Orofacial pain impairs quality of life, and current therapies, limited in efficacy and associated with adverse effects, drive the search for new treatments. Thiophene derivatives exhibit remarkable therapeutic properties, including antinociceptive and anti-inflammatory activities, with recent studies demonstrating superior activity compared to commercial drugs, highlighting their relevance in the design of novel agents. This study investigated the antinociceptive effect of the thiophene derivative 2-[(4-diethylamino-benzylidene)-amino]-5,6,7,8-tetrahydro-4H-cyclohepta[b]thiophene-3-carbonitrile (7CN03) and its possible mechanisms of action. In vivo tests were performed on male mice (n = 6 per group), and nociception was induced by formalin, capsaicin, and glutamate 1 h after treatment. Facial rubbing was used as a parameter to measure nociceptive behavior. 7CN03 exhibited significant action during the neurogenic phase of the formalin test at different doses (1 mg/kg, 0.1 mg/kg, and 0.01 mg/kg), reducing nociceptive behavior by up to 56%. During the inflammatory phase, the 1 mg/kg dose exerted an antinociceptive effect, reducing nociceptive behavior by 32% (p < 0.05). In the glutamate test, 7CN03 blocked nociception by up to 90% (p < 0.001), and in the capsaicin test, it reduced nociceptive behavior by up to 74%. Molecular docking studies predicted higher binding affinity of 7CN03 for µ-opioid (-97.00 Kcal/mol), TRPV1 (-87.79 Kcal/mol), and NMDA (-104.86 Kcal/mol) receptors when compared with cocrystallized ligands. The findings suggest that the evaluated thiophene derivative exhibits an orofacial antinociceptive effect, with a mechanism of action likely mediated by opioid, transient receptor potential vanilloid, and glutamatergic receptors.

The aim of this study was to translate and validate the 14-item Oral Health Impact Profile (OHIP-14) into the Collao variant of the Quechua language for the population of southern Peru. A cross-sectional instrumental study was carried out with 288 participants of both sexes aged between 18 and 65 years (M = 37.53 years; SD = 10.38) who were residents of the department of Cusco, Peru. A convenience sampling method was used. The cultural adaptation process included initial translation, back-translation, review by a committee, focus group evaluation for comprehension and clarity of the items, and a pilot test to ensure the cultural adequacy of the questionnaire. Content validity was evaluated by three evaluators using Aiken's V coefficient. The internal structure was assessed through confirmatory factor analysis (CFA), which tests a three-factor model. The fit indices demonstrated an acceptable model fit, with χ2 = 156.25 (df = 74), RMSEA = 0.062 (90%CI: 0.049-0.076), CFI = 0.999, and TLI = 0.999. The standardized factor loadings exceeded 0.70 for all the items. The corrected item-total correlations indicated strong internal consistency. Reliability was assessed with Cronbach's alpha, which is a high value across all dimensions. The evaluators' assessment demonstrated a high level of agreement for all the items, with Aiken's V coefficient above 0.70. This finding demonstrates that the OHIP-14 is a valid and reliable instrument for the Quechua-speaking population of the Collao variant in Cusco. The study received approval from an ethics committee and adhered to the ethical principles of the Declaration of Helsinki.