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Chromatographic printed array strip (C-PAS) method for cultivar-specific identification of sweetpotato cultivars 'Beniharuka' and 'Fukumurasaki'. 用于甘薯品种“Beniharuka”和“Fukumurasaki”品种特异性鉴定的色谱印刷阵列条(C-PAS)法。
IF 2.4 4区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2023-06-01 Epub Date: 2023-06-28 DOI: 10.1270/jsbbs.22101
Yuki Monden, Maho Kakigi, Emdadul Haque, Tomoyuki Takeuchi, Kazuto Takasaki, Masaru Tanaka

Sweetpotato (Ipomoea batatas) cultivars grown in Japan are highly valued for their excellent sweetness, high quality, and good texture. The export volume of sweetpotato from Japan has been rising rapidly, with a 10-fold increase on a weight basis over the last 10 years. However, since sweetpotato is propagated vegetatively from storage roots, it is easy to cultivate and propagate this crop, prompting concerns that Japanese sweetpotato cultivars/lines are being exported overseas, cultivated without permission, or reimported. Therefore, a rapid and accurate cultivar identification methodology is needed. In this study, we comprehensively analyzed the insertion sites of Cl8 retrotransposon to develop a cultivar identification technique for the Japanese cultivars 'Beniharuka' and 'Fukumurasaki'. These two cultivars were successfully distinguished from other cultivars using a minimum of two marker sets. Using the chromatographic printed array strip (C-PAS) method for DNA signal detection, 'Beniharuka' and 'Fukumurasaki' can be precisely identified using a single strip of chromatographic paper based on multiplex DNA signals derived from the amplicons of the Cl8 insertion sites. Since this method can detect DNA signals in only ~15 minutes, we expect that our method will facilitate rapid, reliable, and convenient cultivar discrimination for on-site inspection of sweetpotato.

在日本种植的甘薯(Ipomoea batatas)品种因其优异的甜味、优质和良好的质地而备受重视。日本红薯的出口量一直在快速增长,在过去10年中,按重量计算增长了10倍。然而,由于红薯是从储存根进行植被繁殖的,因此很容易种植和繁殖这种作物,这引发了人们对日本红薯品种/系出口到海外、未经许可种植或重新进口的担忧。因此,需要一种快速准确的品种鉴定方法。在本研究中,我们综合分析了Cl8反转录转座子的插入位点,以开发日本品种“Beniharuka”和“Fukumurasaki”的品种鉴定技术。使用至少两个标记集成功地将这两个品种与其他品种区分开来。使用色谱印刷阵列条(C-PAS)方法检测DNA信号,可以使用基于Cl8插入位点扩增子的多重DNA信号的单条色谱纸精确识别“Beniharuka”和“Fukumurasaki”。由于该方法可以在约15分钟内检测到DNA信号,我们希望我们的方法将有助于快速、可靠和方便地对甘薯进行品种鉴别,以进行现场检测。
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引用次数: 0
Mutant Tof11 alleles are highly accumulated in early planting-adaptable Japanese summer type soybeans. 突变体Tof11等位基因在早期种植的适应性强的日本夏型大豆中高度积累。
IF 2.4 4区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2023-06-01 Epub Date: 2023-06-28 DOI: 10.1270/jsbbs.22098
Kunihiko Komatsu, Takashi Sayama, Ken-Ichiro Yamashita, Yoshitake Takada

To avoid crop failure because of climate change, soybean (Glycine max (L.) Merrill) cultivars adaptable to early planting are required in western Japan. Because current Japanese cultivars may not be adaptable, genetic resources with high early-planting adaptability, and their genetic information must be developed. In the present study, summer type (ST) soybeans developed for early planting were used as plant materials. We examined their phenological characteristics and short reproductive period as an indicator of early planting adaptability and performed genetic studies. Biparental quantitative trait loci (QTL) analysis of a representative ST cultivar revealed a principal QTL for the reproductive period duration on chromosome 11. The results of resequencing analysis suggested that circadian clock-related Tof11 (soybean orthologue of PRR3) is a candidate QTL. Additionally, all 25 early planting-adaptable germplasms evaluated in this study possessed mutant alleles in Tof11, whereas 15 conventional cultivars only had wild-type alleles. These results suggest that mutant alleles in Tof11 are important genetic factors in the high adaptability to early planting of these soybeans, and thus, these alleles were acquired and accumulated in the ST soybean population.

为了避免气候变化导致的作物歉收,日本西部需要适应早期种植的大豆品种。由于目前的日本品种可能不具有适应性,必须开发早期种植适应性高的遗传资源及其遗传信息。在本研究中,以为早期种植开发的夏型大豆为植物材料。我们检测了它们的表型特征和短繁殖期,作为早期种植适应性的指标,并进行了遗传研究。一个具有代表性的ST品种的双亲数量性状基因座(QTL)分析揭示了11号染色体上生殖期持续时间的主要QTL。重测序分析结果表明,与昼夜节律时钟相关的Tof11(PRR3的大豆直系同源物)是一个候选QTL。此外,本研究评估的所有25个早期种植适应性种质在Tof11中都具有突变等位基因,而15个常规品种只有野生型等位基因。这些结果表明,Tof11中的突变等位基因是这些大豆对早期种植具有高度适应性的重要遗传因素,因此,这些等位基因在ST大豆群体中获得并积累。
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引用次数: 0
Studies of potato resistance to Globodera rostochiensis revealed novel alleles for 57R marker. 对马铃薯抗玫瑰球藻的研究揭示了57R标记的新等位基因。
IF 2.4 4区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2023-06-01 Epub Date: 2023-07-07 DOI: 10.1270/jsbbs.22094
Anna Ivanova-Pozdejeva, Liina Jakobson, Kai Ilves, Agnes Kivistik, Liina Kann, Jekaterina Aida, Liisa Kübarsepp, Terje Tähtjärv, Kristiina Laanemets

Globodera rostochiensis resistance has been an important trait in potato (Solanum tuberosum) breeding for decades. Our aim was to complement phenotypic testing with genetic marker analysis. We analysed the results of G. rostochiensis resistance greenhouse testing in 4601 tubers of 2918 breeding clones from 11 years. Applicability of H1 gene markers TG689 and 57R was compared. We implemented the latter with the positive predictive value of 99.1% and negative predictive value of 60.0% into the breeding scheme. The 57R marker alleles of 22 Estonian cultivars and 470 breeding clones were determined. Two unique 57R alleles, 57R-887 and 57R-1155, were found in Estonian cultivar 'Anti'. The 887 bp allele has two deletions (14 bp and 490 bp) accompanied by several other indels and SNPs within the 57R marker region. The 1155 bp allele has three deletions (7 bp, 20 bp and 210 bp) accompanied by several other indels and SNPs within the same region. Partial resistance to G. rostochiensis in 'Anti' suggests that the newly described alleles could affect the H1-mediated resistance directly or indirectly.

近几十年来,马铃薯(Solanum tuberosum)的抗病性一直是马铃薯育种中的一个重要性状。我们的目的是用遗传标记分析来补充表型测试。我们分析了11年来2918个繁殖无性系中4601个块茎对罗斯托赤霉抗性的温室试验结果。比较H1基因标记TG689和57R的适用性。我们将阳性预测值为99.1%、阴性预测值为60.0%的后者实施到育种方案中。测定了爱沙尼亚22个品种和470个无性系的57R标记等位基因。在爱沙尼亚品种Anti中发现了两个独特的57R等位基因57R-887和57R-1155。887bp等位基因在57R标记区内有两个缺失(14bp和490bp),并伴有其他几个indel和SNPs。1155bp等位基因有三个缺失(7bp、20bp和210bp),并在同一区域内伴有其他几个indel和SNPs。在“Anti”中对罗斯托奇G.rostochensis的部分抗性表明,新描述的等位基因可能直接或间接影响H1介导的抗性。
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引用次数: 0
Development of 12 sets of chromosome segment substitution lines that enhance allele mining in Asian cultivated rice. 在亚洲栽培水稻中开发12套染色体片段替代系,以增强等位基因挖掘。
IF 2.4 4区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2023-06-01 Epub Date: 2023-06-27 DOI: 10.1270/jsbbs.23006
Kazufumi Nagata, Yasunori Nonoue, Kazuki Matsubara, Ritsuko Mizobuchi, Nozomi Ono, Taeko Shibaya, Kaworu Ebana, Eri Ogiso-Tanaka, Takanari Tanabata, Kazuhiko Sugimoto, Fumio Taguchi-Shiobara, Jun-Ichi Yonemaru, Yusaku Uga, Atsunori Fukuda, Tadamasa Ueda, Shin-Ichi Yamamoto, Utako Yamanouchi, Toshiyuki Takai, Takashi Ikka, Katsuhiko Kondo, Tomoki Hoshino, Eiji Yamamoto, Shunsuke Adachi, Jian Sun, Noriyuki Kuya, Yuka Kitomi, Ken Iijima, Hideki Nagasaki, Ayahiko Shomura, Tatsumi Mizubayashi, Noriyuki Kitazawa, Kiyosumi Hori, Tsuyu Ando, Toshio Yamamoto, Shuichi Fukuoka, Masahiro Yano

Many agronomic traits that are important in rice breeding are controlled by multiple genes. The extensive time and effort devoted so far to identifying and selecting such genes are still not enough to target multiple agronomic traits in practical breeding in Japan because of a lack of suitable plant materials in which to efficiently detect and validate beneficial alleles from diverse genetic resources. To facilitate the comprehensive analysis of genetic variation in agronomic traits among Asian cultivated rice, we developed 12 sets of chromosome segment substitution lines (CSSLs) with the japonica background, 11 of them in the same genetic background, using donors representing the genetic diversity of Asian cultivated rice. Using these materials, we overviewed the chromosomal locations of 1079 putative QTLs for seven agronomic traits and their allelic distribution in Asian cultivated rice through multiple linear regression analysis. The CSSLs will allow the effects of putative QTLs in the highly homogeneous japonica background to be validated.

在水稻育种中,许多重要的农艺性状是由多个基因控制的。到目前为止,由于缺乏合适的植物材料来有效检测和验证来自不同遗传资源的有益等位基因,鉴定和选择这些基因所花费的大量时间和精力仍然不足以在日本的实际育种中针对多种农艺性状。为了便于全面分析亚洲栽培稻农艺性状的遗传变异,我们利用代表亚洲栽培稻遗传多样性的供体,开发了12套具有粳稻背景的染色体片段替代系(CSSL),其中11套在相同的遗传背景下。利用这些材料,我们通过多元线性回归分析,概述了亚洲栽培稻7个农艺性状的1079个推定QTL的染色体位置及其等位基因分布。CSSL将允许在高度同质的粳稻背景中验证推定QTL的作用。
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引用次数: 0
Whole-genome sequence analysis of mutations in rice plants regenerated from zygotes, mature embryos, and immature embryos. 从受精卵、成熟胚胎和未成熟胚胎再生的水稻植株突变的全基因组序列分析。
IF 2.4 4区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2023-06-01 Epub Date: 2023-06-15 DOI: 10.1270/jsbbs.22100
Masako Ichikawa, Norio Kato, Erika Toda, Masakazu Kashihara, Yuji Ishida, Yukoh Hiei, Sachiko N Isobe, Kenta Shirasawa, Hideki Hirakawa, Takashi Okamoto, Toshihiko Komari

Somaclonal variation was studied by whole-genome sequencing in rice plants (Oryza sativa L., 'Nipponbare') regenerated from the zygotes, mature embryos, and immature embryos of a single mother plant. The mother plant and its seed-propagated progeny were also sequenced. A total of 338 variants of the mother plant sequence were detected in the progeny, and mean values ranged from 9.0 of the seed-propagated plants to 37.4 of regenerants from mature embryos. The natural mutation rate of 1.2 × 10-8 calculated using the variants in the seed-propagated plants was consistent with the values reported previously. The ratio of single nucleotide variants (SNVs) among the variants in the seed-propagated plants was 91.1%, which is higher than 56.1% previously reported, and not significantly different from those in the regenerants. Overall, the ratio of transitions to transversions of SNVs was lower in the regenerants as shown previously. Plants regenerated from mature embryos had significantly more variants than different progeny types. Therefore, using zygotes and immature embryos can reduce somaclonal variation during the genetic manipulation of rice.

通过全基因组测序研究了从单个母株的受精卵、成熟胚胎和未成熟胚胎再生的水稻(Oryza sativa L.,“Nipponbare”)的体细胞克隆变异。还对母株及其种子繁殖后代进行了测序。在子代中总共检测到338个母株序列的变体,平均值从种子繁殖植物的9.0到成熟胚胎再生剂的37.4不等。利用种子繁殖植物中的变异计算出的1.2×10-8的自然突变率与之前报道的值一致。在种子繁殖植物中的变体中,单核苷酸变体(SNVs)的比例为91.1%,高于先前报道的56.1%,并且与再生剂中的变体没有显著差异。总体而言,如前所述,再生剂中SNV的转换与颠换的比率较低。从成熟胚胎再生的植物比不同的后代类型有更多的变异。因此,在水稻遗传操作过程中,利用受精卵和未成熟胚胎可以减少体细胞克隆变异。
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引用次数: 0
Genome-wide association study of ear tip barrenness in waxy maize. 糯玉米穗尖不育的全基因组关联研究。
IF 2.4 4区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2023-06-01 Epub Date: 2023-06-27 DOI: 10.1270/jsbbs.22056
Xudong Song, Guangfei Zhou, Zhenliang Zhang, Huiming Zhang, Lin Xue, Hui Wang, Mingliang Shi, Huhua Lu, Yuxiang Mao, Guoqing Chen, Xiaolan Huang, Hongjian Zheng, Derong Hao

Ear tip-barrenness (ETB), which results from aborted kernels or infertile florets at the ear tip, is an undesirable factor affecting the yield and quality of waxy maize. To uncover the genetic basis of ETB, a genome-wide association study (GWAS) was conducted using the genotype with 27,354 SNPs and phenotype with three environments. Five SNPs that distributed on chromosomes 1, 3 and 6, were identified to be significantly associated with ETB based on the threshold of false discovery rate (FDR) at 0.05. Among these significant loci, three SNPs were clustered together and colocalized with genomic regions previously reported. The average length of ETB decreased almost linearly from the inbred lines containing no favorable alleles across the three loci (1.75 cm) to those with one (1.18 cm), two (0.94 cm) and three (0.65 cm) favorable alleles. Moreover, three important genes, Zm00001d030028, Zm00001d041510 and Zm00001d038676 were predicted for three significant QTLs, respectively. These results promote the understanding genetic basis for ETB and will be useful for breeding waxy maize varieties with high-quality and high-yield.

穗尖不育(ETB)是影响糯玉米产量和品质的一个不良因素,它是由穗尖败育或小花不育引起的。为了揭示ETB的遗传基础,使用具有27354个SNPs的基因型和具有三种环境的表型进行了全基因组关联研究(GWAS)。根据错误发现率阈值(FDR)为0.05,发现分布在第1、3和6号染色体上的5个SNP与ETB显著相关。在这些重要的基因座中,有三个SNP聚集在一起,并与先前报道的基因组区域共定位。ETB的平均长度从三个位点上不含有利等位基因的自交系(1.75cm)到有一个(1.18cm)、两个(0.94cm)和三个(0.65cm)有利等位突变的自交系几乎呈线性下降。此外,三个重要基因Zm00001d030028、Zm00001d041510和Zm00001C038676分别被预测为三个显著QTL。这些结果有助于加深对ETB遗传基础的认识,对选育优质高产糯玉米品种具有一定的指导意义。
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引用次数: 0
Development of a multicriteria decision-making model for evaluating hybrid offspring in the sweetpotato (Ipomoea batatas L.) breeding process. 在甘薯(Ipomoea batatas L.)育种过程中评估杂交后代的多准则决策模型的开发。
IF 2.4 4区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2023-06-01 Epub Date: 2023-06-27 DOI: 10.1270/jsbbs.22096
Wei Xiang, Kailong Li, Fang Dong, Ya Zhang, Qiang Zeng, Ling Jiang, Daowei Zhang, Yanlan Huang, Liang Xiao, Zhuo Zhang, Chaofan Zhang

Sweetpotato variety breeding is always a long process. Screening of hybrid offspring is dominated by empirical judgment in this process. Data analysis and decision fatigue have been troubling breeders. In recent years, the low-efficiency screening mode has been unable to meet the requirements of sweetpotato germplasm innovation. Therefore, it is necessary to construct a high-efficiency method that can screen germplasms for different usages, for mining elite genotypes, and to create dedicated sweetpotato varieties. In this article, the multicriteria decision-making (MCDM) model was constructed based on six agronomic traits, including fresh root yield, vine length, vine diameter, branch number, root number and the spatial distribution of storage roots, and five quality traits, including dry matter content, marketable root yield, uniformity of roots, starch content and the edible quality score. Among these, the edible quality score was calculated by using fuzzy comprehensive evaluation to integrate the sensory scores of color, odor, sweetness, stickiness and fibrous taste. The MCDM model was compared with the traditional screening method via an evaluation in 25 sweetpotato materials. The interference of subjective factors on the evaluation results was significantly reduced. The MCDM model is more overall, more accurate and faster than the traditional screening method in the selection of elite sweetpotato materials. It could be programmed to serve the breeders in combination with the traditional screening method.

甘薯品种选育是一个漫长的过程。在这个过程中,杂交后代的筛选主要由经验判断决定。数据分析和决策疲劳一直困扰着饲养者。近年来,低效筛选模式已不能满足甘薯种质创新的要求。因此,有必要构建一种高效的方法来筛选不同用途的种质,挖掘优良基因型,并创造专门的甘薯品种。本文基于鲜根产量、藤长、藤径、分枝数、根数和贮藏根空间分布等6个农艺性状,以及干物质含量、商品根产量、根均匀性、淀粉含量和食用质量分数等5个品质性状,构建了多准则决策模型。其中,采用模糊综合评判法,综合颜色、气味、甜味、粘性和纤维味的感官得分,计算出食用质量得分。通过对25种甘薯材料的评价,将MCDM模型与传统筛选方法进行了比较。主观因素对评价结果的干扰显著减少。在甘薯优良材料的筛选中,MCDM模型比传统的筛选方法更全面、更准确、更快。它可以与传统的筛选方法相结合,为育种家服务。
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引用次数: 0
Development of male sterile lines of CMS chilies (Capsicum annuum L.) from F1 hybrids. CMS辣椒(Capsicum annuum L.)杂种雄性不育系的发育。
IF 2.4 4区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2023-04-01 DOI: 10.1270/jsbbs.22042
Maneechat Nikornpun, Kridsada Sukwiwat, Kittisak Wongsing, Jutamas Kumchai

Selfing and crossing methods were used to develop the cytoplasmic male sterility (CMS) lines from 2 elite F1 hybrids of CMS hot chilies. The pungency of the CMS lines was improved by backcrossing with the B cultivar. The first and second backcrossed progenies of the CMS lines showed significantly higher capsaicin contents than the F1 hybrids. One good female line K16 × BBC2 (K16), was selected and backcrossed with 3 good maintainer cultivars, C5, C9 and C0. Some incomplete male sterility of pollens was demonstrated in the F1 hybrids and the 1st backcrossed progenies while the partial sterility disappeared by the stage of the second and third generations of backcrossing. When K16 and P32 were crossed with restorers, fruit yields and yield components of certain F1 hybrids, parental lines and commercial varieties were significantly different. Heterosis of yield and yield components of the F1 hybrid chilies was significant. When K16 was used as a female parent, positive and significant heterosis of the F1 hybrids was the same as P32. Moreover, significant GCA of the restorer lines, C7, C8 and C9, was observed in some horticultural characteristics. Furthermore, significant differences of the specific combining ability of some characteristics were observed in a few F1 hybrids.

采用自交和杂交的方法,选育了2个优质F1辣椒细胞质雄性不育系。通过与B品种回交,提高了CMS系的辛辣性。1代和2代回交子代辣椒素含量显著高于1代。选择1个优良母系K16 × BBC2 (K16),与3个优良保持品种C5、C9和C0回交。杂种1代和回交1代表现出花粉的不完全雄性不育性,到回交2代和3代时花粉的部分不育性消失。当K16和P32与恢复系杂交时,某些F1杂交种、亲本系和商品品种的果实产量和产量成分存在显著差异。杂种辣椒的产量和产量构成均具有显著的杂种优势。以K16为母本时,F1杂种的杂种优势与P32相同。恢复系C7、C8和C9在部分园艺性状上具有显著的GCA。个别F1杂交种在某些性状的特异配合力上存在显著差异。
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引用次数: 0
A novel QTL associated with tolerance to cold-induced seed cracking in the soybean cultivar Toyomizuki. 富水大豆耐冷致种子开裂的新QTL。
IF 2.4 4区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2023-04-01 DOI: 10.1270/jsbbs.22066
Naoya Yamaguchi, Yumi Sato, Fumio Taguchi-Shiobara, Kazuki Yamashita, Michio Kawasaki, Masao Ishimoto, Mineo Senda

Low temperatures after flowering cause seed cracking (SC) in soybean. Previously, we reported that proanthocyanidin accumulation on the dorsal side of the seed coat, controlled by the I locus, may lead to cracked seeds; and that homozygous IcIc alleles at the I locus confer SC tolerance in the line Toiku 248. To discover new genes related to SC tolerance, we evaluated the physical and genetic mechanisms of SC tolerance in the cultivar Toyomizuki (genotype II). Histological and texture analyses of the seed coat revealed that the ability to maintain hardness and flexibility under low temperature, regardless of proanthocyanidin accumulation in the dorsal seed coat, contributes to SC tolerance in Toyomizuki. This indicated that the SC tolerance mechanism differed between Toyomizuki and Toiku 248. A quantitative trait loci (QTL) analysis of recombinant inbred lines revealed a new, stable QTL related to SC tolerance. The relationship between this new QTL, designated as qCS8-2, and SC tolerance was confirmed in residual heterozygous lines. The distance between qCS8-2 and the previously identified QTL qCS8-1, which is likely the Ic allele, was estimated to be 2-3 Mb, so it will be possible to pyramid these regions to develop new cultivars with increased SC tolerance.

大豆开花后低温会导致种子开裂。在此之前,我们报道了原花青素在种皮背面的积累,受I位点控制,可能导致种子开裂;在Toiku 248中,I位点的IcIc等位基因纯合子赋予了SC耐受性。为了发现与SC耐受性相关的新基因,我们对Toyomizuki(基因型II)的SC耐受性的物理和遗传机制进行了评估。对种皮的组织学和质地分析表明,在低温下保持硬度和柔韧性的能力,无论背侧种皮中原花青素的积累,都有助于Toyomizuki的SC耐受性。这表明富井和富田248对SC的耐受性机制存在差异。通过对重组自交系的数量性状位点(QTL)分析,发现了一个与SC耐受性相关的新的、稳定的QTL。在剩余杂合系中证实了这一新的QTL qCS8-2与SC耐受性的关系。qCS8-2与qCS8-1之间的距离估计为2-3 Mb, qCS8-1可能是Ic等位基因,因此有可能将这些区域金字塔化,以开发具有更高耐SC性的新品种。
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引用次数: 0
Molecular mechanism of internode elongation in rice. 水稻节间伸长的分子机制。
IF 2.4 4区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2023-04-01 DOI: 10.1270/jsbbs.22086
Keisuke Nagai, Motoyuki Ashikari

Rice plants that form ventilated tissues, such as aerenchyma in the leaves, stems, and roots, allow for growth in waterlogged conditions (paddy fields), but they cannot breathe and drown in flooded environments where the whole plant body is submerged. However, deepwater rice plants grown in flood-prone areas of Southeast Asia survive in prolonged flooded environments by taking in air through an elongated stem (internode) and leaves that emerge above the water surface, even if the water level is several meters high and flooding continues for several months. Although it has been known that plant hormones, such as ethylene and gibberellins, promote internode elongation in deepwater rice plants, the genes that control rapid internode elongation during submergence have not been identified. We recently identified several genes responsible for the quantitative trait loci involved in internode elongation in deepwater rice. Identification of the the genes revealed a molecular gene network from ethylene to gibberellins in which internode elongation is promoted by novel ethylene-responsive factors and enhances gibberellin responsiveness at the internode. In addition, elucidation of the molecular mechanism of internode elongation in deepwater rice will help our understanding of the internode elongation mechanism in normal paddy rice and contribute to improving crops through the regulation of internode elongation.

水稻植株形成通气组织,如叶片、茎和根中的通气组织,可以在水田条件下生长,但它们不能呼吸,在整个植物体被淹没的水淹环境中淹死。然而,生长在东南亚洪水易发地区的深水水稻,即使水位达到几米高,洪水持续数月,也能通过细长的茎(节间)和露出水面的叶子吸收空气,在长时间的洪水环境中存活下来。虽然已知植物激素,如乙烯和赤霉素,促进深水水稻的节间伸长,但尚未确定在淹没期间控制节间快速伸长的基因。我们最近发现了几个与深水水稻节间伸长数量性状位点有关的基因。这些基因的鉴定揭示了一个从乙烯到赤霉素的分子基因网络,在这个网络中,新的乙烯响应因子促进了节间伸长,增强了赤霉素在节间的响应性。此外,阐明深水水稻节间伸长的分子机制将有助于我们理解正常水稻的节间伸长机制,并有助于通过调控节间伸长来改良作物。
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引用次数: 2
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