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Identification of resistance to Fusarium head blight and molecular cytogenetics of interspecific derivatives between wheat and Psathyrostachys huashanica. 小麦与花沙棘豆种间衍生物的抗枯萎病鉴定及分子细胞遗传学。
IF 2.4 4区 农林科学 Q2 AGRONOMY Pub Date : 2022-06-01 Epub Date: 2022-06-11 DOI: 10.1270/jsbbs.21089
Chenchen Hou, Jing Han, Liangliang Zhang, Qiang Geng, Li Zhao, Shuhui Liu, Qunhui Yang, Xinhong Chen, Jun Wu

Psathyrostachys huashanica is a relative of wheat (Triticum aestivum L.) with many disease resistance genes that can be used to improve wheat disease resistance. In order to enrich the germplasm resources available in wheat genetics and breeding, we assessed Fusarium head blight (FHB) resistance in 45 interspecific derivatives between wheat and Psathyrostachys huashanica during two years from 2017-2018. Two interspecific derivatives comprising, H-34-8-2-6-1 and H-24-3-1-5-19-1 were identified as FHB resistant lines. These two lines were examined based on their morphology and cytogenetics, as well as by genomic in situ hybridization (GISH), fluorescence in situ hybridization (FISH), molecular markers, and 660K genotyping array to determine their genetic construction. The results confirmed H-34-8-2-6-1 as a wheat-P. huashanica 1Ns long arm ditelosomic addition line and H-24-3-1-5-19-1 as a wheat-P. huashanica 2Ns substitution line. Assessments of the agronomic traits showed that H-34-8-2-6 had significantly higher kernel number per spike and self-fertility rate than parent 7182. In addition, compared with 7182, H-24-3-1-5-19-1 had a much lower plant height while the other agronomic traits were relatively similar. The two new lines are valuable germplasm materials for breeding FHB resistance in wheat.

华山拟小麦是小麦(Triticum aestivum L.)的亲缘植物,具有许多抗病基因,可用于提高小麦的抗病性。为了丰富小麦遗传育种的种质资源,在2017-2018年2年间,对45个小麦与花沙刺槐种间衍生物的赤霉病抗性进行了研究。两个种间衍生物h -34-8-2- 1和H-24-3-1-5-19-1被鉴定为FHB抗性品系。利用基因组原位杂交(GISH)、荧光原位杂交(FISH)、分子标记和660K基因分型阵列对这两个品系进行形态学和细胞遗传学分析,确定其遗传结构。结果证实h -34-8-2- 1 -1为小麦- p。华山芥1Ns长臂二染色体附加系与H-24-3-1-5-19-1作为小麦杂种。华山卡2Ns代换系。农艺性状评价表明,H-34-8-2-6的穗粒数和自育率显著高于亲本7182。与7182相比,H-24-3-1-5-19-1株高较低,其他农艺性状基本一致。这两个新品系为小麦抗赤霉病育种提供了宝贵的种质材料。
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引用次数: 0
Genetic variation and genetic control of intraspikelet differences in grain weight and seed dormancy in wild and domesticated emmer wheats. 野生小麦和驯化小麦小穗内粒重和种子休眠差异的遗传变异及遗传控制。
IF 2.4 4区 农林科学 Q2 AGRONOMY Pub Date : 2022-06-01 Epub Date: 2022-06-29 DOI: 10.1270/jsbbs.21060
Shoji Ohta

Seed dormancy, a vital strategy for wild plant species to adapt to an unpredictable environment in their natural habitats, was eliminated from cereals during the domestication process. Intraspikelet differences in grain size and seed dormancy have been observed in wild emmer wheat. To elucidate the genetic variation of these intraspikelet differences and to determine their genetic control, grain weight ratio (first florets/second florets) (GWR), germination rate, and germination index (GI) were analyzed in 67 wild and 82 domesticated emmer wheat accessions, as well as F1 hybrids, F2 populations, and F3-F6 populations derived from reciprocal crosses between wild and domesticated lines. Only the grains on the first florets of two-grained spikelets in wild accessions had varying degrees of dormancy with GI ranging from 0 to 1, which positively correlated with their GWR. This implies that wild emmer populations comprised genotypes with varying degrees of dormancy, including nondormant genotypes. According to segregations observed in F2 populations, the intraspikelet grain weight difference was controlled by two independently inherited loci. Furthermore, low-GWR populations with low or high GI values could be selected in F5 and F6 generations, implying that the major loci associated with dormancy might be independent of intraspikelet grain weight difference.

种子休眠是野生植物适应其自然栖息地不可预测的环境的重要策略,在驯化过程中从谷物中消失了。在野生二粒小麦中观察到小穗内籽粒大小和种子休眠的差异。为阐明这些小穗内差异的遗传变异并确定其遗传控制机制,对67个野生和82个驯化二粒小麦材料,以及杂种F1、F2群体和驯化系正交的F3-F6群体的粒重比(第一小花/第二小花)、发芽率和萌发指数进行了分析。野生材料中只有二粒小穗第一小花的籽粒存在不同程度的休眠,GI值在0 ~ 1之间,与GWR呈正相关。这表明野生二聚体种群包含不同程度休眠的基因型,包括非休眠基因型。根据在F2群体中观察到的分离情况,小穗内粒重差异由两个独立遗传位点控制。此外,在第5代和第6代,可以选择GI值低或高的低gwr群体,这表明与休眠相关的主要位点可能与小穗内粒重差无关。
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引用次数: 0
Quantitative trait loci (QTL) for low temperature tolerance at the young microspore stage in rice (Oryza sativa L.) in Australian breeding material. 澳大利亚水稻(Oryza sativa L.)幼小孢子期耐低温数量性状位点(QTL)
IF 2.4 4区 农林科学 Q2 AGRONOMY Pub Date : 2022-06-01 Epub Date: 2022-06-16 DOI: 10.1270/jsbbs.21096
Christopher Proud, Bradley Campbell, Zuziana Susanti, Shu Fukai, Ian Godwin, Ben Ovenden, Peter Snell, Jaquie Mitchell

Low temperatures at the young microspore stage (YMS) decreases spikelet fertility and is a major limiting factor to rice production in temperate Australia. Low temperature tolerance is a difficult trait to phenotype, hence there is a strong desire for the identification of quantitative trait loci (QTL) for their use in marker-assisted selection (MAS). Association mapping was used in several breeding populations with a known source of low temperature tolerance, Norin PL8, to identify QTL for low temperature tolerance. A novel QTL for spikelet fertility was identified on chromosome 6, qYMCT6.1, in which the Australian variety, Kyeema, was the donor for increased fertility. Additional five genomics regions were identified that co-located with previously reported QTL, two of which have been previously cloned. Additionally, for the first time a QTL for spikelet fertility qYMCT10.1, has been shown to co-locate with the number of dehisced anthers qYMCTF10.1 which increases the shedding of pollen from the anthers. This study revealed one new QTL for low temperature tolerance at YMS in temperate japonica germplasm and identified an additional five previously reported. These QTL will be utilised for MAS in the Australian rice breeding program and may have merit for temperate breeding programs globally.

幼小孢子期(YMS)的低温降低了小穗育性,是澳大利亚温带地区水稻生产的主要限制因素。低温耐受性是一种难以表型化的性状,因此人们强烈希望鉴定数量性状位点(QTL)以用于标记辅助选择(MAS)。利用关联图谱技术,对多个已知低温耐受性来源的育种群体Norin PL8进行了低温耐受性QTL鉴定。在6号染色体qYMCT6.1上发现了一个新的小穗育性QTL,其中澳大利亚品种基马是提高小穗育性的供体。另外鉴定出5个基因组区域与先前报道的QTL共定位,其中2个已被克隆。此外,还首次发现了一个与小穗育性有关的QTL qYMCT10.1,该QTL与开裂花药数量qYMCTF10.1共定位,从而增加了花药花粉的脱落。本研究在温带粳稻种质中发现了一个新的耐低温QTL,并鉴定了另外五个先前报道的QTL。这些QTL将在澳大利亚水稻育种计划中用于MAS,并可能在全球温带育种计划中具有优势。
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引用次数: 1
Hybridization with Fagopyrum cymosum Meisn. as a way to make cultivated Tartary buckwheat (F. tataricum Gaertn.) with grain characteristics typical for common buckwheat (F. esculentum Moench.). 与金荞麦杂交。作为一种方法,使栽培苦荞(F. tararicum Gaertn.)具有普通荞麦(F. esculentum Moench.)典型的籽粒特征。
IF 2.4 4区 农林科学 Q2 AGRONOMY Pub Date : 2022-06-01 Epub Date: 2022-06-17 DOI: 10.1270/jsbbs.21086
Ivan N Fesenko, Nikolay I Bondarev, Olga V Rezunova, Darya E Evsyuticheva, Aleksey N Fesenko

Compared to common buckwheat (F. esculentum), Tartary buckwheat (F. tataricum) is very polymorphic in the type of seeds, but a seed type which is typical for F. esculentum, i.e. triangular seeds with flat sides and clear ribs, has been not found among the polymorphism. However, such seed type is typical for wild species F. cymosum which produces fertile hybrids in crosses with F. tataricum. Embryo rescue based interspecific cross F. esculentum × F. cymosum allowed reveal functional allelism of the genes determining the similar morphs of these species' seeds, i.e. the seed type resulted from mutation(s) at same gene. The gene can be assigned as TAN (triangular). Variation for the seed shell thickness among recessives for the TAN carrying about 12% of F. tataricum genome, together with the shell thickness of a seed from the F1 hybrid F. esculentum × F. cymosum compared to ones of the parents, suggests there are some genes influencing seed shell thickness. Also, it was supported by analyses of seeds characteristics of Tartary-based forms with some share of F. cymosum genetic material. In addition, cross F. tataricum × F. cymosum looks like an effective tool to increase 1000-seed weight of Tartary buckwheat-based breeding material.

与普通荞麦(F. esculentum)相比,苦荞麦(F. tataricum)在种子类型上具有很强的多态性,但在多态性中未发现具有苦荞麦典型的种子类型,即边平棱清的三角形种子。然而,这种种子类型是典型的野生物种F. cymosum与F. tararicum杂交产生可育杂种。基于胚拯救的种间杂交揭示了决定这些物种种子相似形态的基因的功能等位性,即同一基因突变导致的种子类型。该基因可以被指定为TAN(三角形)。携带约12%白杨基因组的TAN的隐性基因间种子壳厚度的差异,以及F1杂交白杨×白杨种子的壳厚度与亲本的壳厚度的差异,表明存在一些影响种子壳厚度的基因。同时,对具有一定遗传物质的酒石桃品种的种子特性分析也支持了这一结论。另外,苦荞杂交是提高苦荞基育种材料千粒重的有效手段。
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引用次数: 0
Erratum: Development of diagnostic molecular markers for marker-assisted breeding against bacterial wilt in tomato. 校正:番茄青枯病诊断分子标记辅助育种的发展。
IF 2.4 4区 农林科学 Q2 AGRONOMY Pub Date : 2022-06-01 DOI: 10.1270/jsbbs.20027e
Alebel Mekuriaw Abebe, Jinwoo Choi, Youngjun Kim, Chang-Sik Oh, Inhwa Yeam, Ill-Sup Nou, Je Min Lee

[This corrects the article DOI: 10.1270/jsbbs.20027.].

[此更正文章DOI: 10.1270/jsbbs.20027.]。
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引用次数: 0
The origin and distribution of 'Kokubu'-type splice-site mutations of the MLO genes in tobacco varieties. 烟草MLO基因“Kokubu”型剪接位点突变的起源和分布。
IF 2.4 4区 农林科学 Q2 AGRONOMY Pub Date : 2022-06-01 Epub Date: 2022-07-01 DOI: 10.1270/jsbbs.22001
Masao Arai, Tomoyuki Komatsu, Hisashi Udagawa, Tomoyuki Tajima, Seiki Sato

The Japanese domestic tobacco (Nicotiana tabacum L.) cultivar 'Kokubu' shows high powdery mildew resistance that is controlled by splice-site mutations of two MILDEW LOCUS O genes, NtMLO1 and NtMLO2. We investigated the existence of the same NtMLO1/2 splice mutations in the genomes of various tobacco varieties cultivated in Japan and other countries. In total, 14 Japanese domestic cultivars, which were mainly distributed in Kagoshima, had splice-site mutations in both NtMLO1 and NtMLO2. In addition, tobacco cultivars containing only the NtMLO1 splice-site mutation were found in various tobacco production areas in Japan, but no cultivars with only the NtMLO2 splice-site mutation were detected. Moreover, the NtMLO1 splice-site mutation was detected in native Asian, Oriental and cigar tobacco varieties. Consequently, we speculate that these powdery mildew-resistant tobacco cultivars were generated relative recently in the Kagoshima area when a spontaneous mutation occurred at the NtMLO2 splice site in a cultivar already containing the NtMLO1 splice-site mutation and that the NtMLO1 splice-site mutation occurred during the early period of tobacco seed dissemination from the Americas to Asia and Japan.

日本国产烟草(Nicotiana tabacum L.)品种“国部”(Kokubu)具有较高的白粉病抗性,这是由两个mildew位点O基因NtMLO1和NtMLO2剪接位点突变控制的。我们研究了在日本和其他国家栽培的不同烟草品种的基因组中是否存在相同的NtMLO1/2剪接突变。14个主要分布在鹿儿岛的日本国内品种均存在NtMLO1和NtMLO2剪接位点突变。此外,在日本各烟草产区均发现了仅含有NtMLO1剪接位点突变的烟草品种,但未发现仅含有NtMLO2剪接位点突变的烟草品种。此外,在亚洲、东方和雪茄烟草品种中检测到NtMLO1剪接位点突变。因此,我们推测这些抗白粉病的烟草品种是最近在鹿鹿岛地区产生的,当时一个已经含有NtMLO1剪接位点突变的品种的NtMLO2剪接位点发生了自发突变,而NtMLO1剪接位点突变发生在烟草种子从美洲传播到亚洲和日本的早期。
{"title":"The origin and distribution of 'Kokubu'-type splice-site mutations of the <i>MLO</i> genes in tobacco varieties.","authors":"Masao Arai,&nbsp;Tomoyuki Komatsu,&nbsp;Hisashi Udagawa,&nbsp;Tomoyuki Tajima,&nbsp;Seiki Sato","doi":"10.1270/jsbbs.22001","DOIUrl":"https://doi.org/10.1270/jsbbs.22001","url":null,"abstract":"<p><p>The Japanese domestic tobacco (<i>Nicotiana tabacum</i> L.) cultivar 'Kokubu' shows high powdery mildew resistance that is controlled by splice-site mutations of two <i>MILDEW LOCUS O</i> genes, <i>NtMLO1</i> and <i>NtMLO2</i>. We investigated the existence of the same <i>NtMLO1/2</i> splice mutations in the genomes of various tobacco varieties cultivated in Japan and other countries. In total, 14 Japanese domestic cultivars, which were mainly distributed in Kagoshima, had splice-site mutations in both <i>NtMLO1</i> and <i>NtMLO2</i>. In addition, tobacco cultivars containing only the <i>NtMLO1</i> splice-site mutation were found in various tobacco production areas in Japan, but no cultivars with only the <i>NtMLO2</i> splice-site mutation were detected. Moreover, the <i>NtMLO1</i> splice-site mutation was detected in native Asian, Oriental and cigar tobacco varieties. Consequently, we speculate that these powdery mildew-resistant tobacco cultivars were generated relative recently in the Kagoshima area when a spontaneous mutation occurred at the <i>NtMLO2</i> splice site in a cultivar already containing the <i>NtMLO1</i> splice-site mutation and that the <i>NtMLO1</i> splice-site mutation occurred during the early period of tobacco seed dissemination from the Americas to Asia and Japan.</p>","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"72 3","pages":"248-256"},"PeriodicalIF":2.4,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9653193/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40488225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Transferring of clubroot-resistant locus CRd from Chinese cabbage (Brassica rapa) to canola (Brassica napus) through interspecific hybridization. 通过种间杂交将大白菜(Brassica rapa)抗性基因座CRd转移到油菜(Brassica napus)。
IF 2.4 4区 农林科学 Q2 AGRONOMY Pub Date : 2022-06-01 Epub Date: 2022-06-24 DOI: 10.1270/jsbbs.21052
Zongxiang Zhan, Nadil Shah, Ru Jia, Xiaonan Li, Chunyu Zhang, Zhongyun Piao

Clubroot, caused by Plasmodiophora brassicae is one of the most severe threats to brassica species in China and worldwide. Breeding for clubroot resistant varieties is one of the best ways to overcome this disease. In this study, we introduced clubroot resistance (CR) gene CRd from Chinese cabbage (85-74) into elite Brassica napus inbred line Zhongshuang 11 through interspecific hybridization and subsequent backcrossing with whole-genome molecular marker-assisted selection (MAS). The resistant test of CRd to P. brassicae isolates was evaluated in the greenhouse as well as in field conditions. Close linkage markers and the whole-chromosome background marker selection approach improved the recovery rate from 78.3% in BC1 to 100% in BC3F1. The improved clubroot-resistant variety, Zhongshuang11R, was successfully selected in the BC3F2 generation. The greenhouse and field resistant tests revealed that Zhongshuang11R was resistant to P. brassicae pathotypes. The agronomic characteristics of Zhongshuang11R were similar to those of its recurrent parental line, including oil content, composition of fatty acid, plant height, primary effective branches, grain yield per plant and thousand-seed weight. In addition, the oil quality could satisfy the quality requirements for commercial rapeseed oil. Our results will enrich the resistant resources of canola and will certainly accelerate clubroot resistance breeding programs in B. napus.

由芸苔菌引起的棒根病是中国乃至世界范围内对芸苔属植物最严重的威胁之一。选育抗病品种是克服该病的最佳途径之一。本研究通过种间杂交和全基因组分子标记辅助选择(MAS)回交,将大白菜(85-74)抗棒根病基因(CRd)导入优质甘蓝型油菜自交系中双11。在温室条件和田间条件下,对CRd对芸苔菌的抗性试验进行了评价。紧密连锁标记和全染色体背景标记选择方法使BC1的回收率从78.3%提高到BC3F1的100%。在BC3F2代中成功选育了抗棒虫病的改良品种中双11r。温室和田间抗性试验表明,中双11r对芸苔属病原菌具有抗性。中双11r的油分、脂肪酸组成、株高、一次有效枝数、单株籽粒产量、千粒重等农艺性状与其亲本相似。油的质量满足商品菜籽油的质量要求。我们的研究结果将丰富油菜的抗性资源,并必将加快油菜根瘤菌抗性育种的步伐。
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引用次数: 1
Classification of Southeast Asian mints (Mentha spp.) based on simple sequence repeat markers. 基于简单序列重复标记的东南亚薄荷属植物分类。
IF 2.4 4区 农林科学 Q2 AGRONOMY Pub Date : 2022-04-01 Epub Date: 2022-03-09 DOI: 10.1270/jsbbs.21058
Yuri Fukui, Moeko Saito, Natsuno Nakamura, Taichi Mizuno, Shuichi Sato, Mayu Tsukuda, Saori Nakaoka, Keita Tsuboi, Azusa Sasaki, Kouji Kuramochi, Panida Boonyaritthongchai, Nichapat Kaewmanee, Krit Thirapanmethee, Mullika Traidej Chomnawang, Bhanubong Bongcheewin, Thuy Linh Nguyen, Huong Lan Thi Nguyen, Huong Thi Le, Shigehisa Okamoto, Takako Nakamura, Yasushi Nakamura, Nakao Kubo

Mentha is a complex genus encompassing many species as a consequence of their interspecific hybridization and polyploidy. Southeast Asian mints have been poorly distinguished though they are widely used for culinary and medical purposes. In this study, we have analyzed Southeast Asian mints and known varieties as well as a related Lamiaceae species (Nepeta sp.) using simple sequence repeat (SSR) markers and leaf morphology. Two types of mints were clearly distinguished based on their venation pattern and leaf shape index. We developed 12 SSR markers that allowed good amplification in the Mentha and another Lamiaceae species. In the SSR-based phylogram, the Mentha lines could be delimited into groups I-VI. The Southeast Asian mints divided into groups I and II, and the phylogram separated most of the available species, with groups I and II containing the known species M. × cordifolia and M. arvensis, respectively. The separation of the two groups was supported by a population structure analysis. The SSR markers developed in this study enabled the simultaneous classification of mints and will help improve our understanding of the genetic composition of known mint varieties and as yet unclassified Southeast Asian mints.

薄荷是一个复杂的属,包括许多种,由于它们的种间杂交和多倍体的结果。尽管东南亚薄荷被广泛用于烹饪和医疗目的,但它们的区别却很差。本研究利用SSR标记和叶片形态分析了东南亚薄荷、已知薄荷品种以及一种薄荷科植物。根据薄荷的脉状形态和叶形指数,可以清楚地区分两种薄荷。我们开发了12个SSR标记,可以在薄荷和另一个Lamiaceae物种中进行良好的扩增。在基于ssr的系统图谱中,薄荷系可划分为I-VI类群。东南亚薄荷属植物分为I类和II类,其系统谱区分了大部分可用种,其中I类和II类分别含有已知种M. x cordifolia和M. arvensis。种群结构分析支持了这两组的分离。本研究开发的SSR标记实现了薄荷的同时分类,将有助于提高我们对已知薄荷品种和尚未分类的东南亚薄荷的遗传组成的理解。
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引用次数: 0
Contribution of the grain size QTL GS3 to yield properties and physiological nitrogen-use efficiency in the large-grain rice cultivar 'Akita 63'. 籽粒大小QTL GS3对大粒稻‘秋田63’产量性状和生理氮素利用效率的贡献
IF 2.4 4区 农林科学 Q2 AGRONOMY Pub Date : 2022-04-01 Epub Date: 2022-03-08 DOI: 10.1270/jsbbs.21043
Mitsuhiro Obara, Yoshihiro Kaneta, Ikuko Kodama, Sinichi Matsumoto, Tomohiko Kawamoto, Keiki Ishiyama, Tadahiko Mae, Amane Makino

The development of crop varieties with high nitrogen-use efficiency (NUE) is thought to be important in achieving sustainable cereal crop production. The high yield large-grain rice cultivar Oryza sativa L. 'Akita 63' (temperate japonica) has high physiological NUE (PNUE) for grain yield (GY). Our previous study revealed that a large-grain allele of GS3 is present in 'Akita 63'. Here, we verified the influence of GS3 on the yield properties and PNUE for GY in 'Akita 63'. The frequency distribution of brown rice length in F2 crosses of 'Iwate 75' and 'Akita 63' showed a continuous distribution that could be explained by GS3. A near-isogenic line was developed to substitute the GS3 segment of 'Koshihikari', which harbours a normal-sized grain allele, in the genetic background of 'Akita 63' and the line was designated as Akita63NILGS3-Koshihikari. Compared with Akita63NILGS3-Koshihikari, 'Akita 63' exhibited a significantly increased grain length, single brown grain weight and GY, although no significant differences were observed in the nitrogen content and above-ground biomass per unit of cultivated area. These results indicate that the GS3 large-grain allele is a contributing factor to high PNUE for GY in 'Akita 63'. These findings will facilitate the development of nitrogen-efficient rice varieties.

开发氮利用效率高的作物品种对实现谷类作物的可持续生产具有重要意义。高产大粒稻品种Oryza sativa L。‘秋田63’(温带粳稻)具有较高的籽粒产量生理氮肥利用率。我们之前的研究发现,在秋田63中存在一个GS3的大粒等位基因。在此,我们验证了GS3对‘秋田63’GY产量特性和PNUE的影响。‘岩手75’和‘秋田63’F2杂交组合的糙米长度频率分布呈连续分布,可以用GS3解释。为替代“秋田63”遗传背景中含有正常大小粒等位基因的“Koshihikari”的GS3片段,构建了一个接近等基因的品系,命名为“Akita63NILGS3-Koshihikari”。与Akita63NILGS3-Koshihikari相比,‘秋田63’的粒长、单粒重和GY显著增加,但氮素含量和单位面积地上生物量差异不显著。上述结果表明,GS3大粒等位基因是秋田63籽粒光合效率高的一个因素。这些发现将促进氮肥高效水稻品种的开发。
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引用次数: 3
ITRAQ-based quantitative proteomic analysis of japonica rice seedling during cold stress. 基于itraq的低温胁迫下粳稻幼苗蛋白质组学定量分析
IF 2.4 4区 农林科学 Q2 AGRONOMY Pub Date : 2022-04-01 Epub Date: 2022-02-02 DOI: 10.1270/jsbbs.21081
Dongjin Qing, Guofu Deng, Yinghua Pan, Lijun Gao, Haifu Liang, Weiyong Zhou, Weiwei Chen, Jingcheng Li, Juan Huang, Ju Gao, Chunju Lu, Hao Wu, Kaiqiang Liu, Gaoxing Dai

Low temperature is one of the important environmental factors that affect rice growth and yield. To better understand the japonica rice responses to cold stress, isobaric tags for a relative and absolute quantification (iTRAQ) labeling-based quantitative proteomics approach was used to detected changes in protein levels. Two-week-old seedlings of the cold tolerant rice variety Kongyu131 were treated at 8°C for 24, 48 and 72 h, then the total proteins were extracted from tissues and used for quantitative proteomics analysis. A total of 5082 proteins were detected for quantitative analysis, of which 289 proteins were significantly regulated, consisting of 169 uniquely up-regulated proteins and 125 uniquely down-regulated proteins in cold stress groups relative to the control group. Functional analysis revealed that most of the regulated proteins are involved in photosynthesis, metabolic pathway, biosynthesis of secondary metabolites and carbon metabolism. Western blot analysis showed that protein regulation was consistent with the iTRAQ data. The corresponding genes of 25 regulated proteins were used for quantitative real time PCR analysis, and the results showed that the mRNA level was not always parallel to the corresponding protein level. The importance of our study is that it provides new insights into cold stress responses in rice with respect to proteomics and provides candidate genes for cold-tolerance rice breeding.

低温是影响水稻生长和产量的重要环境因子之一。为了更好地了解粳稻对冷胁迫的响应,采用等压标签相对和绝对定量(iTRAQ)标记为基础的定量蛋白质组学方法来检测蛋白质水平的变化。将耐寒水稻品种空育131 2周龄幼苗在8℃条件下处理24、48和72 h,提取组织总蛋白,进行定量蛋白质组学分析。共检测到5082个蛋白进行定量分析,其中289个蛋白被显著调控,其中冷应激组相对于对照组特异性上调169个蛋白,特异性下调125个蛋白。功能分析显示,大部分受调控蛋白参与光合作用、代谢途径、次生代谢产物的生物合成和碳代谢。Western blot分析显示蛋白调控与iTRAQ数据一致。将25个调控蛋白对应基因进行实时荧光定量PCR分析,结果显示mRNA水平并不总是与对应蛋白水平平行。本研究的重要意义在于从蛋白质组学的角度对水稻的冷胁迫反应提供了新的认识,并为水稻的耐寒性育种提供了候选基因。
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引用次数: 2
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Breeding Science
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