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A diatom quality index from a diatom-based total phosphorus inference model 基于硅藻总磷推断模型的硅藻质量指数
Pub Date : 2007-04-10 DOI: 10.1080/15555270701263234
Gerald V. Sgro, E. Reavie, J. Kingston, Amy R. Kireta, M. Ferguson, N. Danz, J. Johansen
A diatom quality index to assess diatom community impairment in the nearshore wetlands of the Laurentian Great Lakes was developed from a diatom-based total phosphorus (TP) weighted average inference model. The index is calculated with a weighted average equation using species optima standardized to a 1–10 scale and species tolerance standardized to a 1–3 scale. Multiple regression analysis revealed a moderate fit (R 2 = 0.63) between site scores of the selected index and GIS derived watershed characteristics (agriculture, soils, and industrial facilities). These index scores more closely fit watershed characteristics than the diatom inferred TP (R 2 = 0.59). In a regression tree analysis, soil permeability separated higher index scores from lower scores identifying this variable as an important interaction factor in the analysis. The diatom quality index can be a powerful tool for analyzing habitat quality in the Great Lakes and can communicate the link between quantifiable diatom assemblage response with watershed-level disturbance.
基于硅藻总磷(TP)加权平均推理模型,建立了评价Laurentian五大湖近岸湿地硅藻群落损害的硅藻质量指数。该指数采用物种最优标准化为1-10级,物种容忍度标准化为1-3级的加权平均公式计算。多元回归分析显示,所选指数的站点得分与GIS衍生的流域特征(农业、土壤和工业设施)之间存在适度拟合(r2 = 0.63)。这些指标得分比硅藻推断TP更接近流域特征(r2 = 0.59)。在回归树分析中,土壤渗透性将较高的指数得分从较低的得分中分离出来,从而确定该变量是分析中重要的相互作用因素。硅藻质量指数可以作为分析五大湖生境质量的有力工具,并能传达定量硅藻组合响应与流域扰动之间的联系。
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引用次数: 19
Editorial: A Framework for Using Bioindicators in Ecosystem Management 社论:生物指标在生态系统管理中的应用框架
Pub Date : 2007-04-10 DOI: 10.1080/15555270701263127
D. Reagan
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引用次数: 2
Research Note: Quantification of Increase in Hepatic CYP1A1 mRNA by Real-Time RT-PCR after Exposure of the European Eel (Anguilla anguilla) to a Diesel Oil Water Soluble Fraction 研究说明:将欧洲鳗鲡(Anguilla Anguilla)暴露于柴油水溶性馏分后,用实时荧光定量pcr方法定量肝脏CYP1A1 mRNA的增加
Pub Date : 2007-04-10 DOI: 10.1080/15555270701263002
É. Aubry, C. Cagnon, Y. Lalanne, C. Mouchès
Editors' note: Research Notes is a new section now being added to the EBI format. Its purpose is to provide a forum for the publication of short research communications that provide important information but, due to their restricted focus, are not formatted in the manner of full research papers. All papers published under this heading will be peer reviewed. In order to follow the effects of pollutants such as polycyclic aromatic hydrocarbons (PAHs), polychlorobenzenes (PCBs), and dioxins upon the European eel, we have developed an assay that quantifies hepatic cytochrome P4501A1 (CYP1A1) mRNA levels using real-time reverse transcription polymerase chain reaction (real-time RT-PCR). In this study, European eels were exposed to a diesel water soluble fraction (DWSF) in order to mimic environmental exposure to a mixture of organic pollutants. CYP1A1 mRNA levels were measured after 3, 6, 12, 24, 48, 72 and 156 h of exposure. The 7-ethoxyresorufin O-deethylase (EROD) activity was measured in parallel to confir...
编者注:研究笔记是一个新的部分,现在被添加到EBI格式。它的目的是为提供重要信息的简短研究通讯的出版提供一个论坛,但由于它们的重点有限,不像完整的研究论文那样格式化。所有在此标题下发表的论文都将经过同行评审。为了跟踪多环芳烃(PAHs)、多氯苯(PCBs)和二恶英等污染物对欧洲鳗鱼的影响,我们开发了一种利用实时逆转录聚合酶链反应(real-time RT-PCR)定量肝细胞色素P4501A1 (CYP1A1) mRNA水平的测定方法。在这项研究中,欧洲鳗鱼暴露于柴油水溶性组分(DWSF)中,以模拟环境暴露于有机污染物混合物中。在暴露3、6、12、24、48、72和156 h后检测CYP1A1 mRNA水平。平行测定了7-乙氧基间苯二酚o -去乙基酶(EROD)活性,以证实其有效性。
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引用次数: 1
Architectural Changes in the Gill Morphology of the Freshwater Fish, Esomus danricus as Potential Biomarkers of Copper Toxicity Using Automated Video Tracking System 使用自动视频跟踪系统研究淡水鱼鳃形态的结构变化作为铜毒性的潜在生物标志物
Pub Date : 2007-04-10 DOI: 10.1080/15555270701263259
S. Vutukuru, J. S. Pauleena, J. Rao, Y. Anjaneyulu
Biomarker responses at the histological level to xenobiotics are gaining a priority research interest. The 96h LC50 of copper sulfate to Esomus danricus is found to be 5.5 mg/L and its toxic effects on fish gills are critically examined in situ. Gills (pair III) of fish exposed to 5.5 mg/L and 0.55 mg/L of copper sulfate and control without toxicant were placed in normal saline medium and, after rinsing, placed on a glass slide and observed under a Polyvar, Reichert-Jung light microscope attached to Ethovision-version 2.3 (Noldus Information Technology, Netherlands) through a Sony CCD IRIS camera (Model No: SSC-M370CE). Damage to secondary gill lamellae with loss of shape, necrosis, clubbing and fusion, shaft breakage, atrophy, curvature of tips, thick mucous sheathing and degeneration of rakers are observed in fish exposed to 5.5 mg/L of copper sulfate. Primary and secondary gill lamellae are affected with loss of original shape, loss of alignment in rakers with hyper secretion of mucous in fish exposed ...
生物标志物在组织学水平上对外源性药物的反应正在获得优先的研究兴趣。发现硫酸铜对鱼鳃的96h LC50为5.5 mg/L,并对其对鱼鳃的毒性作用进行了严格的原位检测。将暴露于5.5 mg/L和0.55 mg/L硫酸铜及无毒物对照的鱼鳃(III对)置于生理盐水培养基中,冲洗后置于载玻片上,通过索尼CCD IRIS相机(型号:SSC-M370CE)在Ethovision-version 2.3 (Noldus Information Technology, Netherlands)安装的Polyvar, Reichert-Jung光学显微镜下观察。暴露于5.5 mg/L硫酸铜的鱼,二级鳃片损伤,形状丧失,坏死,棒状和融合,轴断裂,萎缩,尖端弯曲,粘液鞘变厚和耙变性。初级和次级鳃片受到影响,失去了原来的形状,在耙失去对齐与粘液分泌过多的鱼暴露。
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引用次数: 10
Roundtable Discussion Groups Summary Papers: Environmental Bio-Indicators in Coral Reef Ecosystems: The Need to Align Research, Monitoring, and Environmental Regulation 圆桌讨论小组总结论文:珊瑚礁生态系统中的环境生物指标:协调研究、监测和环境监管的必要性
Pub Date : 2007-03-16 DOI: 10.1080/15555270701263200
P. Sammarco, P. Hallock, J. Lang, R. S. Legore
Editors' Note: At the 14th International Conference on Environmental Bioindicators (14th ICEBI) held in Linthicum Heights, Maryland, USA on 24–26 April 2006, the Conference Chairs and Program Committee initiated the Roundtable Discussion Groups as a regular feature of this and future conferences. The Discussions are designed to generate focused debate around key topic areas, led by academic, government and industry experts, and are structured to produce definitive papers for peer review and publication in EBI's first-quarter issue of each publication year, albeit this year the papers will be published over the first two issues of 2007. The three Roundtables of the 14th ICEBI posed questions revolving around the chosen topic areas of Mercury Bioindicators, Marine Ecosystem-level Indicators, and Regulatory and Policy Uses of Bioindicators, and moved from “what we know” to “where we need to go” and “what are the policy implications from our discussions and conclusions.” The following paper on coral reef indi...
编者按:在2006年4月24日至26日在美国马里兰州林西克姆高地举行的第14届国际环境生物指标会议(第14届ICEBI)上,会议主席和计划委员会发起了圆桌讨论小组,作为本届和未来会议的常规特色。讨论的目的是在学术、政府和行业专家的领导下,围绕关键主题领域展开集中辩论,并形成最终论文供同行评议,并发表在EBI每个出版年度的第一季度,尽管今年的论文将在2007年前两期发表。第14届ICEBI的三个圆桌会议围绕汞生物指标、海洋生态系统级指标和生物指标的监管和政策使用等选定的主题领域提出了问题,并从“我们知道什么”转向“我们需要去哪里”和“我们的讨论和结论对政策有什么影响”。下面这篇关于珊瑚礁的文章…
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引用次数: 6
Cell Proliferation Detected with Flow Cytometric Cell Cycle Analysis and Immunohistochemical Detection of Proliferating Cell Nuclear Antigen (PCNA) from Somatic Tissues of Eastern Oysters, Crassostrea virginica ∗ 流式细胞术细胞周期分析和免疫组化检测东方牡蛎体细胞组织增殖细胞核抗原(PCNA)的研究
Pub Date : 2006-12-12 DOI: 10.1080/15555270600868174
J. Jenkins, J. Lapeyre
Two novel biomarker of response assays were developed and compared for use with bivalves. Bivalve mollusks are often used as bioindicators to monitor contaminant body burdens and are employed globally in pollution monitoring and as sentinels of environmental quality. The prevalence of proliferating cells in tissues of the eastern oyster, Crassostrea virginica, (n = 10) was investigated by using immunohistochemical staining of proliferating cell nuclear antigen (PCNA) and by flow cytometric analysis of DNA in cell cycle phases. The PCNA protein is highly conserved and central to DNA synthesis in dividing cells. This protein was detected by using a commercial antibody in the histological staining of gills, mantle, digestive gland, stomach, and labial palps. Cells obtained from these same organs and from the hearts of the oysters were used for flow cytometry (FCM). The proportions of nuclei in the S plus G2/M and G2/M fractions were compared with percentages of PCNA-positive cells scored through microscopy. ...
开发了两种新的生物标志物反应测定方法,并将其用于双壳类动物进行了比较。双壳类软体动物经常被用作监测污染物体负荷的生物指标,在全球范围内被用于污染监测和环境质量的哨兵。采用增殖细胞核抗原(PCNA)免疫组化染色和细胞周期DNA流式细胞术分析,研究了东牡蛎(Crassostrea virginica, n = 10)组织中增殖细胞的流行情况。PCNA蛋白是高度保守的,是分裂细胞中DNA合成的核心。该蛋白通过商业抗体在鳃、套、消化腺、胃和唇瓣的组织学染色中检测到。从这些器官和牡蛎心脏中获得的细胞用于流式细胞术(FCM)。将S + G2/M和G2/M组中细胞核的比例与显微镜下pcna阳性细胞的百分比. ...进行比较
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引用次数: 1
Ion (Organic Acid and Base) Transporters and Homeostasis: A Review with Reference to its Usefulness as Biomarkers 离子(有机酸和碱)转运体与体内平衡:综述及其作为生物标志物的用途
Pub Date : 2006-12-12 DOI: 10.1080/15555270600860692
P. Mandal
Organisms are continuously exposed to a great variety of xenobiotics via food and environment. Evolution has equipped the body with a plethora of protecting systems to defend itself against the potentially harmful effects of these compounds. One of the important defense mechanisms include the active extrusion of xenobiotics by commonly shared transport proteins, mainly located in kidney, liver and intestine. An attempt has been made to understand the structural and functional properties of organic anion transporters (OAT1, OAT2, OAT3) and organic cation transporters (OCTN1, OCTN2, OCT1, OCT2, OCT3). These transporters share a predicted 12-transmembrane domain (TMD) structure with a large extracellular loop between TMD1 and TMD2, carrying potential N-glycosylation sites. Conserved amino acid motifs revealed a relationship to the sugar transporter family within the major facilitator superfamily. Following heterologous expression, most OATs transported the model anion p-aminohippurate (PAH). OAT1, but not OA...
生物体通过食物和环境不断接触到各种各样的外源物质。进化已经为身体配备了大量的保护系统来保护自己免受这些化合物的潜在有害影响。其中一个重要的防御机制是由共同转运蛋白(主要位于肾、肝和肠)主动挤出外源物质。对有机阴离子转运体(OAT1、OAT2、OAT3)和有机阳离子转运体(OCTN1、OCTN2、OCT1、OCT2、OCT3)的结构和功能特性进行了研究。这些转运蛋白共享一个预测的12跨膜结构域(TMD)结构,在TMD1和TMD2之间有一个大的细胞外环,携带潜在的n -糖基化位点。保守的氨基酸基序揭示了与主要促进剂超家族中的糖转运蛋白家族的关系。异种表达后,大多数燕麦转运模型阴离子对氨基马粪酸(PAH)。OAT1,但不是OA…
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引用次数: 1
Editorial: Indicator Hierarchy and Scaling∗ 社论:指标层次与标度
Pub Date : 2006-12-12 DOI: 10.1080/15555270600868109
J. Messer
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引用次数: 1
Heavy Metals in the Sea Cucumber Holothuria tubulosa (Echinodermata) from the Mediterranean Posidonia oceanica Ecosystem: Body Compartment, Seasonal, Geographical and Bathymetric Variations 地中海Posidonia oceanica生态系统中管状海参(Holothuria tubulosa)中的重金属:体室、季节、地理和水深变化
Pub Date : 2006-12-08 DOI: 10.1080/15555270601034388
M. Warnau, S. Dutrieux, G. Ledent, A. R. Y. Baena, P. Dubois
The concentrations of Cd, Cu, Fe, Pb and Zn were measured in holothuroids Holothuria tubulosa collected during different seasons and at several depths in three N W Mediterranean Posidonia oceanica meadows (Calvi, Corsica; Ischia, Italy; Marseille, France). Metals were found to be selectively distributed among the holothuroid body compartments (i.e. body wall, gut, and haemal system). Among the sources of variation taken into account, the major part of the variability observed in metal concentrations was associated to the body compartment factor, then to the seasonal one and, finally, to the geographical and bathymetric factors. Our results suggest that, if one uses H. tubulosa as a bioindicator species for surveying and monitoring metal contamination in the Mediterranean P. oceanica meadows, attention should be paid to compare only concentrations measured in the same body compartments of individuals collected during the same period of the year. Body compartments that should be preferably considered for me...
本文测定了在地中海西北三处海洋波西多尼亚草甸不同季节和不同深度采集的管状Holothuria Holothuria tubulosa中Cd、Cu、Fe、Pb和Zn的浓度。坐骨、意大利;法国马赛的)。发现金属有选择性地分布于甲状腺细胞腔室(即体壁、肠道和血液系统)。在考虑到的变化来源中,观察到的金属浓度变化的主要部分与身体间隔因素有关,其次是季节因素,最后是地理和水深因素。研究结果表明,如果将管状水蛭作为一种生物指示物种,用于调查和监测地中海海洋水蛭草甸的金属污染,应注意只比较同一时期收集的个体在同一身体区室中测量的浓度。对我来说,最好考虑一下身体隔间……
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引用次数: 44
Effect of Cadmium Exposure on Serum Amylase Activity in Cadmium Electroplating Workers 镉暴露对镉电镀工人血清淀粉酶活性的影响
Pub Date : 2006-12-08 DOI: 10.1080/15555270601025758
R. Kalahasthi, R. Rao, Rajan Bagalur Krishna Murthy, M. Kumar
Objective. The effect of cadmium exposure on serum amylase activity has been monitored in cadmium plating workers of Bangalore City (India).Methods. Fifty subjects using cadmium during electroplating formed the study group. An equal number of age–sex matched subjects working in administrative sections formed the control group. Urinary cadmium levels were determined by using a flameless atomic absorption spectrophotometer. Serum amylase activity was determined by using spectrophotometric method. Results. A significant increase of urine cadmium and serum amylase activity was noted in the subjects of the study group as compared with the subjects in the control group. The level of serum amylase activity was positively and significantly associated with cadmium levels in urine. Multiple regression analysis was used to assess the effect of cadmium exposure on serum amylase activity. The analysis showed that the study subjects who had urine cadmium levels greater than 5-μg/ g of creatinine and cadmium exposure we...
目标。监测了镉暴露对印度班加罗尔镀镉工人血清淀粉酶活性的影响。50名在电镀过程中使用镉的受试者组成研究组。在行政部门工作的相同数量的年龄性别匹配的受试者组成对照组。用无焰原子吸收分光光度计测定尿镉水平。用分光光度法测定血清淀粉酶活性。结果。与对照组相比,研究组受试者的尿镉和血清淀粉酶活性显著增加。血清淀粉酶活性水平与尿中镉含量呈显著正相关。采用多元回归分析评价镉暴露对血清淀粉酶活性的影响。分析表明,研究对象尿镉水平大于5 μg/ g的肌酐和镉暴露我们…
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引用次数: 4
期刊
Environmental bioindicators
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