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[Chimeric SHA-D domain ("SH3-Bergerac"): 3D-structure and dynamics studies in solution]. [嵌合SHA-D结构域(“SH3-Bergerac”):溶液中的3d结构和动力学研究]。
Pub Date : 2010-07-01
V S Khristophorov, D A Prokhorov, M A Timchenko, Iu A Kudrevatykh, L V Gushchina, V V Filimonov, V P Kutyshenko

Protein SHA-D of "SH3-Bergerac" chimeric proteins family was constructed by substitution of beta-turn N47-D48 in spectrin SH3-domain by KATANDKTYE amino acid sequence. Structural and dynamics properties of SHA-D in solution were studied by with the help of high-resolution NMR. The extension of SHA-D polypeptide chain in comparison with wild type of protein WT-SH3 (~ 17%) practically doesn't affect almost the total molecule topology. 3D-structure of SHA-D is practically identical to the proteins of "SH3-Bergerac" family. However there are some differences in dynamic characteristics in the region of substitution. The G52D substitution in SHA-D protein results in a destabilization of the region insertion where the conditions for conformational exchange appear. Destabilization further affects the entire SHA- D molecule making its structure more labile.

利用KATANDKTYE氨基酸序列取代sh3结构域β -turn N47-D48,构建了“SH3-Bergerac”嵌合蛋白家族的SHA-D蛋白。利用高分辨率核磁共振研究了SHA-D在溶液中的结构和动力学性质。与野生型蛋白WT-SH3(~ 17%)相比,SHA-D多肽链的延伸几乎不影响分子的总拓扑结构。SHA-D的3d结构与“SH3-Bergerac”家族的蛋白质几乎相同。然而,在替代区域,两者的动态特性存在一些差异。SHA-D蛋白中G52D的取代导致构象交换条件出现的区域插入不稳定。不稳定进一步影响整个SHA- D分子,使其结构更加不稳定。
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引用次数: 0
[Oxidation of dermatan sulfate with a NaOCl-NaBr-2,2,6,6-tetramethylpiperidine-1-oxyl reagent in the water medium]. [用naocl - nabr -2,2,6,6-四甲基哌啶-1-氧试剂在水介质中氧化硫酸皮聚糖]。
Pub Date : 2010-05-01
I Iu Ponedel'kina, E A Khaĭbrakhmanova, V N Odinokov, L M Khalilov, U M Dzhemilev

The oxidation of primary hydroxyl groups in dermatan sulfate with the NaOCl-NaBr-2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) reagent in an aqueous alkaline environment was carried out for the first time. Modified dermatan sulfates containing hydrated aldehyde (15-50%) and carboxyl (25-100%) groups were obtained.

首次用naocl - nabr -2,2,6,6-四甲基哌啶-1-氧(TEMPO)试剂在碱性水环境下氧化硫酸皮丹中的伯羟基。得到了含有水合醛(15-50%)和羧基(25-100%)基团的改性皮聚糖硫酸盐。
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引用次数: 0
[Synthesis, structures, and acute toxicity of gossypol nonsymmetrical aldehyde derivatives]. [棉酚不对称醛衍生物的合成、结构和急性毒性]。
Pub Date : 2010-05-01
K Z Tiliabaev, F G Kamaev, N L Vypova, A M Iuldashev, B T Ibragimov, S A Talipov

Nonsymmetrical aldehyde derivatives of gossypol, a yellow polyphenolic pigment of cottonseed, were synthesized by reactions with ammonia, aniline, 4-aminoantipyrine, and barbituric acid. Their structures were determined by UV spectrophotometry and IR and (1)H NMR spectroscopy methods. Their acute toxicities in white mice were compared with those of gossypol and the corresponding symmetrical analogues. It was demonstrated that in general, the fewer free aldehyde groups that contained the gossypol derivative, the lower its acute toxicity. Only in the case of a nonsymmetrical gossypol derivative bearing a 4-aminoantipyrine residue did we observe a deviation from the above correlation: its symmetrical counterpart was even more toxic, but still less toxic than gossypol.

以氨水、苯胺、4-氨基安替比林和巴比妥酸为原料,合成了棉籽黄色多酚色素棉酚的不对称醛类衍生物。采用紫外分光光度法、红外光谱法和(1)氢核磁共振光谱法测定了它们的结构。并与棉酚及其相应的对称类似物对小白鼠的急性毒性进行了比较。结果表明,一般情况下,含棉酚衍生物的游离醛越少,其急性毒性越低。只有在含有4-氨基安替比林残基的非对称棉酚衍生物的情况下,我们才观察到与上述相关性的偏差:其对称对应物的毒性甚至更大,但毒性仍然比棉酚小。
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引用次数: 0
[2'-aldehyde oligonucleotides: synthesis and use for affinity modification of DNA-recognizing proteins]. [2'-醛寡核苷酸:合成及其在dna识别蛋白亲和修饰中的应用]。
Pub Date : 2010-05-01
E A Khomiakova, E V Kazanova, E M Zubin, E A Kubareva, N V Molochkov, E M Riazanova, T S Oretskaia

Oligonucleotides with 1,2-diol grouping were prepared from 2'-O-[2-(2,3-dihydroxypropyl)amino-2-oxo-ethyl]uridine 3'-phosphoramidite. The thermal stability of modified DNA duplexes and their ability to form complexes with the p50 subunit of the NF-kappaB transcription factor and (cytosine-5)-DNA methyltransferase SsoII were studied. The periodate oxidation of the l,2-diol grouping of the oligonucleotides resulted in reactive 2'-aldehyde derivatives. The opportunity of their use for the affinity modification of DNA-recognizing proteins was studied.

以2′-o -[2-(2,3-二羟丙基)氨基-2-氧乙基]尿苷3′-磷酰胺为原料制备了具有1,2-二醇基团的寡核苷酸。研究了修饰DNA双链的热稳定性及其与NF-kappaB转录因子p50亚基和(胞嘧啶-5)-DNA甲基转移酶SsoII形成复合物的能力。寡核苷酸的1,2 -二醇基团的高碘酸氧化产生活性的2'-醛衍生物。研究了它们用于dna识别蛋白亲和修饰的机会。
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引用次数: 0
[Isolation and determination of activity of IgA1 protease from Neisseria meningitidis]. [脑膜炎奈瑟菌IgA1蛋白酶的分离及活性测定]。
Pub Date : 2010-01-01
E Iu Iagudaeva, L S Zhigis, O A Razguliaeva, V S Zueva, E E Mel'nikov, V P Zubov, L V Kozlov, A M Bichucher, O V Kotel'nikova, A P Alliluev, A E Avakov, L D Rumsh

A method of the isolation and purification of IgAl protease from a culture of Neisseria meningitidis serogroup A has been developed. Three inactivated intermediates of the production of the meningococcal vaccine, a culture liquid, as well as a supernatant and sediment obtained by the precipitation of bacterial cells by cetavlon, served as a starting material. The purity of IgA1 protease was determined by SDS-PAGE. An immunoenzyme assay for determining the IgA1 protease activity has been devised. The yield of the enzyme with a specific activity of 0.5 to 4 million units/mg from 103 g of the cetavlon precipitate (40 l of culture liquid) was about 600 mug. It was shown that IgAl protease isolated from serogroup A meningococcus is capable of protecting experimental animals (mice) infected with meningococcus of serogroup B.

建立了一种从脑膜炎奈瑟菌血清A群培养物中分离纯化IgAl蛋白酶的方法。三种生产脑膜炎球菌疫苗的灭活中间体,一种培养液,以及由cetavlon沉淀细菌细胞获得的上清液和沉淀物,作为起始材料。用SDS-PAGE法测定IgA1蛋白酶的纯度。设计了一种测定IgA1蛋白酶活性的免疫酶测定法。从103g的cetavlon沉淀物(40l培养液)中得到比活性为50 ~ 400万单位/mg的酶,产率约为600马克。结果表明,从A群脑膜炎球菌中分离的IgAl蛋白酶对感染B群脑膜炎球菌的实验动物(小鼠)具有保护作用。
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引用次数: 0
[STAT1: a many-sided transcription factor]. [STAT1:一种多侧转录因子]。
Pub Date : 2010-01-01
I A Kostanian, A V Vonarshenko, V M Lipkin

Cell homeostasis is regulated by numerous signaling proteins. Their main task is to process extracellular signals and activate the intracellular cascades of reactions that lead to the modulation of gene activity. One of the important signaling systems is the STAT family, which is comprised of seven members. Various STATs operate as effective transcription factors delivering cytokine and growth factor signals to the nucleus. The first found and most studied member of this family is STAT1. This review summarizes modern data on the role of STAT1 in the maintenance of cellular homeostasis, and special attention is paid to this protein in the proliferation and apoptosis of immune system cell processes. It is shown that disturbances in the functionality of this molecule might contribute to oncogenesis.

细胞稳态是由多种信号蛋白调控的。它们的主要任务是处理细胞外信号并激活导致基因活性调节的细胞内级联反应。其中一个重要的信号系统是STAT家族,它由七个成员组成。各种stat作为有效的转录因子将细胞因子和生长因子信号传递到细胞核。这个家族中最早被发现和研究最多的成员是STAT1。本文综述了STAT1在维持细胞稳态中的作用的现代数据,并特别关注该蛋白在免疫系统细胞过程中的增殖和凋亡。研究表明,对该分子功能的干扰可能有助于肿瘤的发生。
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引用次数: 0
[Comparison of polypeptide compositions from individual Agelena orientalis spider venoms]. [东方艾格莉娜蜘蛛毒液中多肽成分的比较]。
Pub Date : 2010-01-01
Iu M Shliapnikov, S A Kozlov, A A Fedorov, E V Grishin

Spider venoms are peculiar combinatory libraries of polypeptide molecules that specifically affect various cell targets. However, the question has remained up to now regarding whether the observed diversity of the polypeptides results from the synthesis of the complete library of these molecules by each individual spider or is due to the peculiarity of each zooid producing a limited set of components. We studied the composition of the mixed venom taken from several dozens of zooids of the Central Asian species of the Agelena orientalis and compared it with the venoms of 20 individual spiders of this species. The venoms were qualitatively and quantitatively analyzed by HPLC, mass spectrometry, and amino acid sequencing. It was shown that the individual venoms contain a lesser number of polypeptide components in comparison with the mixed venom and, in addition, differ from each other by the component composition. The set of components produced by single zooids is relatively narrow, and on the whole is a set identical to that of the mixed venom. The polypeptides with a high content in the venom were structurally characterized and compared with the amino acid sequences deduced from the cDNA library of this species.

蜘蛛毒液是一种特殊的多肽分子组合文库,可以特异性地影响各种细胞目标。然而,迄今为止的问题仍然是,观察到的多肽的多样性是由于每只蜘蛛合成了完整的这些分子文库,还是由于每只蜘蛛产生有限成分的特性。我们研究了从中亚种Agelena orientalis的几十种动物中提取的混合毒液的成分,并将其与该种蜘蛛的20个个体的毒液进行了比较。采用高效液相色谱法、质谱法和氨基酸测序对毒液进行定性和定量分析。结果表明,与混合毒液相比,单个毒液含有较少数量的多肽成分,并且在成分组成上彼此不同。单个动物产生的一组成分相对较窄,总体上与混合毒液的一组成分相同。对毒液中含量较高的多肽进行了结构表征,并与从该物种cDNA文库中推导出的氨基酸序列进行了比较。
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引用次数: 0
[Molecular chaperones]. (分子伴侣’)。
Pub Date : 2010-01-01
E E Mel'nikov, T V Rotanova

Chaperones are unique remodeling proteins that participate in a great number of intracellular processes and are involved in the correction of protein structure, the prevention of the aggregation of misfolded proteins, the destruction of protein aggregates, and also the unfolding of native protein targets for their translocation across a membrane. In addition to this, Chaperones assist in the dismantling of active oligomers into inactive unfolded monomers for their subsequent photolytic degradation and the assembly of folded subunits into protein assemblies and specific complexes. Data on the structure and functioning of molecular chaperones from five basic families are summarized in the review.

伴侣蛋白是一种独特的重塑蛋白,参与大量的细胞内过程,参与蛋白质结构的纠正,防止错误折叠蛋白质的聚集,破坏蛋白质聚集体,以及展开天然蛋白质靶标以实现其跨膜易位。除此之外,伴侣蛋白还有助于将活性低聚物分解成无活性的未折叠单体,以供其随后的光解降解,并将折叠的亚基组装成蛋白质组件和特定复合物。综述了5个基本科分子伴侣蛋白的结构和功能方面的研究进展。
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引用次数: 0
[Sulfonium derivatives of thioxanthenone, a new class of photodetritylating agents for microarray oligonucleotide synthesis]. [硫代蒽酮的磺化衍生物,一类用于微阵列寡核苷酸合成的新型光去三烷基化剂]。
Pub Date : 2010-01-01
A N Siniakov, A A Riabinin, G A Maksakova, V V Shelkovnikov, V A Loskutov, E V Vasil'ev, N V Shekleina

The usability of a new class of photo acids, namely, sulfonium hexaphosphates based on thioxanthenone, for the removal of the dimethoxytrityl protective group in the process of oligonucleotide synthesis has been studied in order to search for new detritylating agents for microarray oligodeoxyribonucleotide synthesis. 2,4-Diethyl-9-oxo-10-(4-heptyloxyphenyl)-9H-thioxanthenium hexafluorophosphate has been successfully used for the solid-phase synthesis of (dT)(10).

为了寻找微阵列低聚脱氧核糖核苷酸合成的新型去三烷基化试剂,研究了一类新的光酸,即基于硫代蒽酮的六磷酸磺酸在低聚核苷酸合成过程中去除二甲氧基三烷基保护基团的可用性。2,4-二乙基-9-氧-10-(4-庚氧基苯基)- 9h -六氟磷酸硫代蒽醌已成功用于固相合成(dT)(10)。
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引用次数: 0
[Synthetic peptide immunorphin as an instrument for studying nonopioid beta-endorphin receptor]. [合成肽免疫啡肽作为研究非阿片样β -内啡肽受体的工具]。
Pub Date : 2010-01-01
Iu A Kovalitskaia, E V Navolotskaia

Research results of the synthetic decapeptide SLTCLVKGFY (the author's term is immunorphin) corresponding to the 364-373 sequence of G heavy-chain human immunoglobulin are summarized. Special attention is paid to the interaction between immunorphin and a nonopioid (insensitive to the opioid antagonist naloxone) beta-endorphin receptor. Using radioligand analysis, data were found regarding the distribution and functions of a nonopioid beta-endorphin receptor in human and animal bodies and the binding characteristics of immunorphin with a nonopioid receptor.

综述了合成的G重链人免疫球蛋白364-373序列对应的十肽SLTCLVKGFY(作者术语为immunorphin)的研究结果。特别注意免疫啡和非阿片(对阿片拮抗剂纳洛酮不敏感)β -内啡肽受体之间的相互作用。利用放射配体分析,我们发现了一种非阿片样β -内啡肽受体在人和动物体内的分布和功能,以及免疫啡肽与非阿片样受体的结合特性。
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引用次数: 0
期刊
Bioorganicheskaia khimiia
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