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In vitro delivery of curcumin with cholesterol-based cationic liposomes. 姜黄素与胆固醇基阳离子脂质体的体外递送。
Pub Date : 2013-07-01
N Apiratikul, T Penglong, K Suksen, S Svasti, A Chairoungdua, B Yingyongnarongkula

A new cholesterol-based cationic lipid was synthesized; liposomes prepared on its basis were evaluated as drug delivery vehicles for curcumin. Free and liposome-encapsulated curcumin cytotoxicity against HeLa, A549, HepG2, K562 and 1301 cell lines was assessed. Liposomal curcumin with ED50 values ranging from 2.5-10 microM exhibited 2-8 times higher cytotoxicity than free curcumin. The synthetic cholesterol-based cationic lipid also enhanced cellular uptake of curcumin into tested cells. Cationic liposome alone showed low cytotoxicity at high doses with ED50 values of 90-210 microM.

合成了一种新型胆固醇基阳离子脂质;在此基础上制备的脂质体作为姜黄素给药载体进行了评价。研究了游离姜黄素和脂质体包膜姜黄素对HeLa、A549、HepG2、K562和1301细胞株的细胞毒性。ED50值为2.5 ~ 10 μ m的脂质体姜黄素的细胞毒性比游离姜黄素高2 ~ 8倍。合成的以胆固醇为基础的阳离子脂质也增强了姜黄素进入测试细胞的细胞摄取。阳离子脂质体在高剂量下具有较低的细胞毒性,ED50值为90 ~ 210 μ m。
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引用次数: 0
[Glycosilated derivatives of substituted hydroxylamine. II. Phase transfer synthesis and investigation of glycosyl transfer reaction of glucosaminides of substituted hydroxylavine]. 取代羟胺的糖硅氧烷化衍生物。2取代羟基丙烷氨基葡萄糖的相转移合成及糖基转移反应研究[j]。
Pub Date : 2013-07-01 DOI: 10.7868/s0132342313030081
V O Kur'ianov, A A Lushchik, T A Chupakhina

1-(2-Acetamido-3,4,6,-tri-O-acetyl-2-deoxy-beta-D-glucopyranosyloxy)-benzotriazole reacted in boiling dichloromethane, in the presence of Luis acids as a promotors with primary and secondary aliphatic and cycloaliphatic alcohols and diisopropilidene galactose with alkyl-O-1,2-trans-glucosaminides formation. It was shown that the other glucosaminides of substituted hydroxylamine are not participated in this reaction. Structures of glucosaminides were identify by 1H-NMR-spectroscopy and comparison with known compounds.

1-(2-乙酰氨基-3,4,6,- 3 - o-乙酰-2-脱氧- β -d -葡萄糖吡喃氧基)-苯并三唑在煮沸的二氯甲烷中反应,在路易斯酸作为启动子的存在下与伯、仲脂肪醇和环脂肪醇和二异丙烯半乳糖与烷基- o- 1,2-反式氨基葡萄糖生成。结果表明,取代羟胺的其他氨基葡萄糖不参与该反应。利用核磁共振光谱对氨基葡萄糖进行了结构鉴定,并与已知化合物进行了比较。
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引用次数: 0
[A compact microarray for sub-typing of influenza A virus]. [用于甲型流感病毒分型的紧凑型微阵列]。
Pub Date : 2013-05-01 DOI: 10.7868/s0132342313030123
V A Riabinin, E V Kostina, A N Siniakov

A universal oligonucleotide hybridazation microchip 6 x 5 spot (4 x 4 mm) for influenza A virus subtyping was suggested, functioning on a principle one spot--one subtype. This microchip with additional printing quality control is a prototype of a biosensor for detection of influenza A virus and typing of 15 subtypes of hemagglutinin and 9 subtypes of neuraminidase.

提出了一种用于甲型流感病毒亚型分型的通用寡核苷酸杂交微芯片6 × 5点(4 × 4 mm),其工作原理是一点一亚型。这种带有附加打印质量控制的微芯片是一种用于检测甲型流感病毒和分型15种血凝素亚型和9种神经氨酸酶亚型的生物传感器的原型。
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引用次数: 0
[Synthesis of aminopropylaminoderivatives of betulinic and oleanolic acids]. [白桦酸和齐墩果酸的氨基丙基氨基衍生物的合成]。
Pub Date : 2013-05-01 DOI: 10.7868/s0132342313020061
G V Giniiatullina, O B Kazakova, N I Medvedeva, I V Sorokina, N A Zhukova, T G Tolstikova, G A Tolstikov

On the basis of betulinic and oleanolic acids triterpenoids with different with different amine fragments: (3-aminopropoxy)-, 3-acetyl-(3-aminopropyl)amino-, 6-[bis(3-aminopropyl)amino]hexylamino-, (3-aminopropyl)-4-aminosulfonyl-4-phenylamino- at positions C3 and C28 were synthesized. It is shown that betulonic acid amide with 4,4'-diaminodiphenylsulfonic substituent don't render antitumor effect in mice with transplantable Lewis lung carcinoma, but possess significant anti-inflammatory activity.

在白桦脂酸和齐墩果酸的基础上,合成了3-氨基丙氧基-、3-乙酰基-(3-氨基丙基)氨基-、6-[双(3-氨基丙基)氨基]己基氨基-、(3-氨基丙基)-4-氨基磺酰基-4-苯基氨基-等具有不同胺片段的三萜化合物。结果表明,含4,4′-二氨基二苯磺酸取代基的苯二酸酰胺对可移植Lewis肺癌小鼠无抗肿瘤作用,但具有明显的抗炎活性。
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引用次数: 0
Synthesis and synergistic, additive inhibitory effects of novel spiro derivatives against ringworm infections. 新型螺旋衍生物的合成及其对癣感染的协同加性抑制作用。
Pub Date : 2013-05-01
Gajanand Sharma, Richa Sharma, Anshu Dandia, Preeti Bansal

An environmentally benign solvent free synthesis of various spiro-1,4-dihydropyridines (1,4-DHPs) incorporating 2-oxindole/piperidines is performed in 5-8 min with reasonable purity in 80-90% yield under microwave irradiation using montmorillonite KSF as an inorganic solid support. The reaction is found to be general with respect to various cyclic carbonyl compounds, e.g. cyclohexanone, substituted indole-2,3-dione, and piperidinone derivatives. In our study, these compounds were also found effective against dermatophytes and other fungal organisms. Our results suggest that novel spiro derivatives can be used for the treatment of dermatophytosis or ringworm infections.

在微波辐射下,以KSF蒙脱土为无机固体载体,在5 ~ 8 min内合成了含2-氧吲哚/哌啶的各种螺-1,4-二氢吡啶(1,4- dhps),纯度合理,产率为80 ~ 90%。对于各种环羰基化合物,如环己酮、取代吲哚-2,3-二酮和哌啶酮衍生物,发现该反应是普遍的。在我们的研究中,这些化合物也被发现对皮肤真菌和其他真菌生物有效。我们的结果表明,新的螺旋衍生物可用于治疗皮肤癣或癣感染。
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引用次数: 0
[Synthetic conformational antigen, simulating the extracellular part of M2-muscarinic receptor and its reactivity with sera from patients with idiopathic arrhythmias]. [合成构象抗原,模拟m2 -毒蕈碱受体细胞外部分及其与特发性心律失常患者血清的反应性]。
Pub Date : 2013-05-01 DOI: 10.7868/s013234231303010x
M E Pal'keeva, M V Sidorova, A S Molokoedov, A A Az'muko, Zh D Bespalova, T V Sharf, E N Mamochkina, E E Efremov, M M Rogova, N A Mironova, K A Zykov, S P Golitsyn

Linear peptides corresponding to fragment 83-98 of the first loop and fragments 168-192 and 171-182 of the second extracellular loops of M2-muscarinic receptor (marker of early cardiac disorders and arrhythmias) were synthesized by Fmoc-SPPS method. A new conformational antigen was synthesized by method of selective ligation of linear peptides by disulfide bond with native localization. Peptides were studied in reaction with sera from patients with idiopathic arrhythmias. A new conformational antigen was recognized by sera from patients with idiopathic arrhythmias with high reactivity.

采用Fmoc-SPPS法合成了m2 -毒毒碱受体(早期心功能障碍和心律失常的标志)胞外环第83 ~ 98段和第168 ~ 192段、第171 ~ 182段对应的线状肽。采用二硫键选择性连接线性多肽的方法合成了一种新的构象抗原。研究了多肽与特发性心律失常患者血清的反应。一种新的构象抗原在特发性心律失常患者血清中被识别,具有高反应性。
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引用次数: 0
Synthesis and biological evaluation of novel isoxazolo[4,3-e]indoles as antibacterial agents. 新型异恶唑[4,3-e]吲哚类抗菌药物的合成及生物学评价。
Pub Date : 2013-03-01
Mehdi Pordel, Amin Abdollahi, Bahareh Razavi

The synthesis of a new series of 8-bromo-6-alkyl-1-aryl-6H-isoxazolo[4,3-e]indole derivatives is described. All the newly synthesized compounds were screened for their antibacterial activity against Escherichia coli HB101, Staphylococcuse aureus pathogens (methicillin resistant S. aureus and methicillin susceptible S. aureus), Pseudomonas aeruginosa, and Bacillus subtilis; also MIC values of these compounds were determined.

报道了一系列新的8-溴-6-烷基-1-芳基-6- h -异恶唑[4,3-e]吲哚衍生物的合成。新合成的化合物对大肠杆菌HB101、金黄色葡萄球菌病原菌(耐甲氧西林金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌)、铜绿假单胞菌和枯草芽孢杆菌的抑菌活性进行了筛选;测定了这些化合物的MIC值。
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引用次数: 0
[Unusual amidation reaction of Asn-containing glycopeptide antibiotics using the coupling reagent PyBOP]. [偶联剂PyBOP对含砷糖肽抗生素的异常酰胺化反应]。
Pub Date : 2013-03-01 DOI: 10.7868/s0132342313020097
E N Olsuf'eva, S E Solov'eva, M I Reznikova, A M Korolev, M N Preobrazhenskaia

The coupling reagent PyBOP is widely used for the synthesis of different peptides and their amides, particularly for carboxamides of glycopeptide antibiotics of vancomycin or teicoplanin groups. The amidation reaction of the peptide core of the glycopeptide antibiotic eremomycin (I) with highly reactive amines in the presence of PyBOP is usually not accompanied by the formation of side products. However, the amidation of I with bulky amines (e.g., decyl amine and adamantyl amine) in the presence of PyBOP and Et3N or di-(i-Pr)2EtN (pH - 8.5) yielded N-unsubstituted carboxamide of eremomycin (Ia) as an admixture. The reaction of (I) or vancomycin (II) with an excess of PyBOP and Et3N (pH - 8.5) without addition of an amine or ammonia gave a mixture of products which contained higher amounts of the corresponding N-unsubstituted carboxamides (-20%). The structures of the samples of Ia and vancomycin amide (IIa) were proved by 1H NMR and ESI MS methods and confirmed by comparing with the authentic samples.

偶联试剂PyBOP广泛用于合成各种多肽及其酰胺,特别是万古霉素或替可普兰蛋白类糖肽类抗生素的羧胺。在PyBOP存在下,糖肽类抗生素eremomycin (I)的肽核与高活性胺的酰胺化反应通常不伴有副产物的形成。然而,在PyBOP和Et3N或二(I - pr)2EtN (pH - 8.5)存在的情况下,用大体积胺(例如,癸胺和金刚烷胺)酰胺化I,得到作为混合物的n -未取代的伊雷霉素(Ia)羧酰胺。(I)或万古霉素(II)与过量的PyBOP和Et3N (pH - 8.5)反应,而不添加胺或氨,得到的混合物中相应的n -未取代的羧酰胺含量较高(-20%)。通过1H NMR和ESI MS对Ia和万古霉素酰胺(IIa)的结构进行了验证,并与正品进行了对比。
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引用次数: 0
[Investigation of fluorescent components of drug "heparin" and its complexing with phenylalanine, tyrosine and tryptophan]. [药物“肝素”的荧光成分及其与苯丙氨酸、酪氨酸和色氨酸络合的研究]。
Pub Date : 2013-01-01 DOI: 10.7868/s0132342313010090
S S Astakhov, V M Iunusov, M V Sultanbaev, G Kh Akhmadeeva, M S Iunusov

Using spectral-luminescent and spectrophotometric methods a composition of aminoacidic (AA) and protein components of commercial drug of heparin (Hep) as well as its interaction with Trp, Tyr and Phe was studied. The impurities of aromatic aminoacids Phe, Tyr and elastin protein was revealed in drug of heparin. The quenching of fluorescence (FL) of Trp, Tyr and Phe with heparin was studied and the Stern-Volmer Constants (K) showing the stability of its complexes with aminoacids were determined. The stability of complexes AA...Hep increases in the series K (Trp...Hep) = 19 +/- 2 M(-1) < K (Tyr...Hep) = 39 +/- 3 M(-1) < K (Phe...Hep) = 710 +/- 70 M(-1), that, probably, determines dominating contribution of Phe impurity in heparin and the absence of that of tryptophan. It was assumed, that animal elastin, which is different from human one can provoke allergic reactions up to anaphylactic shock.

采用光谱发光和分光光度法研究了市售药物肝素(Hep)的氨基酸(AA)和蛋白质组分的组成及其与色氨酸、酪氨酸和苯丙氨酸的相互作用。肝素类药物中发现了芳香族氨基酸苯丙氨酸、酪氨酸和弹性蛋白的杂质。研究了Trp、Tyr和Phe在肝素作用下的猝灭荧光(FL),并测定了其与氨基酸配合物稳定性的Stern-Volmer常数(K)。配合物AA…Hep增加的序列为K (Trp…Hep) = 19 +/- 2 M(-1) < K (Tyr…Hep) = 39 +/- 3 M(-1) < K (Phe…Hep) = 710 +/- 70 M(-1),这可能决定了肝素中以Phe杂质为主而色氨酸缺失的原因。据推测,动物弹性蛋白与人类弹性蛋白不同,可引起过敏反应直至过敏性休克。
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引用次数: 0
[Bacterial pigment prodigiosin and its genotoxic effects]. 细菌色素prodigiosin及其基因毒性作用。
Pub Date : 2013-01-01 DOI: 10.7868/s0132342312060048
I D Gur'ianov, N S Karamova, D V Iusupova, O I Gnezdilov, L A Koshkarova

The prodigiosin preparation was isolated and purified from Serratia marcescens ATCC 9986, using chromatographic methods. The analysis of the preparation by TLC, NMR-spectrometry and mass-spectrometry allowed to confirm the red pigment fraction as the prodigiosin and detect its purity. Originally, the specific features of the toxic and genotoxic effects of prodigiosin and the possibility of induction of mutations by pigment in the cells of Salmonella typhimurium TA 100 (Ames test) and chromosome damage of mammalian erythroblasts have been determined.

采用色谱法从粘质沙雷菌ATCC 9986中分离纯化了芥子皂苷制剂。通过薄层色谱、核磁共振光谱和质谱分析,确定了红色素部分为神子红素,并测定了其纯度。最初,研究人员已经确定了鼠伤寒沙门氏菌TA 100细胞中的毒性和基因毒性作用的具体特征以及色素诱导突变的可能性(Ames试验)和哺乳动物红细胞的染色体损伤。
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引用次数: 0
期刊
Bioorganicheskaia khimiia
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