Journal of hazardous materials letters最新文献_第2页

Emerging contaminants in stormwater: Tire-derived chemicals, pharmaceuticals, and heavy metals detected in a United Arab Emirates extreme weather event 雨水中出现的污染物：在阿拉伯联合酋长国的极端天气事件中检测到的轮胎衍生化学品、药品和重金属

IF 8.1 Q1 ENGINEERING, ENVIRONMENTAL

Journal of hazardous materials letters

Pub Date : 2025-08-07 DOI: 10.1016/j.hazl.2025.100162

Jonathan Navarro Ramos , Lara Dronjak , Sofian Kanan , Md Maruf Mortula , Joshua S. Wallace , Fatin Samara , Ning Dai , Diana S. Aga

Climate change is associated with the intensification of weather patterns, including extreme weather events of historically arid regions such as the United Arab Emirates (UAE). On April 16–17, 2024, the UAE experienced a severe storm with unusually heavy precipitation, which resulted in widespread urban flooding, contaminated floodwaters, and the potential for cross-contamination in water distribution systems. Ten floodwater samples from around the American University of Sharjah, UAE, were analyzed for the presence of emerging contaminants (e.g., tire-derived chemicals, pharmaceuticals, and personal care products (PPCPs)) and selected metals. Tire-derived chemicals were detected at varying concentrations: N-(1,3-Dimethylbutyl)-N′-phenyl-p-phenylenediamine (6PPD) (<detection limit - 26 ng/L), 6PPD-quinone (20–270 ng/L), 1,3-diphenylguanidine (DPG) (490–14,340 ng/L), and hexa(methoxymethyl)melamine (HMMM) (70–15,800 ng/L). Four PPCPs (caffeine, cotinine, acetaminophen, and lidocaine) were detected at lower concentrations (42–779 ng/L, 6–179 ng/L, 4–196 ng/L, and 1–7 ng/L, respectively). Significantly higher amounts of all tire-derived chemicals and PPCPs were observed in samples from outside the campus. Metals were also detected: aluminum (92–218 µg/L), iron (49–349 µg/L), potassium (160–3860 µg/L), manganese (3 µg/L), and barium (1–7 µg/L). Correlation analysis revealed a strong positive correlation between 6PPD-quinone and HMMM (r = 0.893, p = 0.000507), and among acetaminophen, caffeine, cotinine, and lidocaine (r = 0.501–0.980, and p < 0.05), suggesting shared sources stemming from high vehicular activity and raw wastewater overflow. These findings emphasize the need for arid regions to implement targeted stormwater management and monitoring strategies during extreme weather events to address floodwater-driven mobilization of environmental contaminants.

气候变化与天气模式的加剧有关，包括阿拉伯联合酋长国等历史上干旱地区的极端天气事件。2024年4月16日至17日，阿联酋经历了一场异常强降水的强风暴，导致广泛的城市洪水，洪水污染以及供水系统交叉污染的可能性。对来自阿联酋沙迦美国大学周围的10个洪水样本进行了分析，以确定新出现的污染物（例如，轮胎衍生的化学品、药品和个人护理产品（PPCPs））和选定金属的存在。轮胎衍生化学品的检测浓度不同：N-（1,3-二甲基丁基）-N ' -苯基-对苯二胺（6PPD）（检出限为26 ng/L）、6PPD-醌（20-270 ng/L）、1,3-二苯胍（DPG）（490-14,340 ng/L）和六（甲氧基甲基）三聚氰胺（HMMM）（70-15,800 ng/L）。四种PPCPs（咖啡因、可替宁、对乙酰氨基酚和利多卡因）在较低浓度（分别为42-779 ng/L、6-179 ng/L、4-196 ng/L和1-7 ng/L）下检测到。在校园外的样本中观察到的所有轮胎衍生化学物质和PPCPs的含量明显更高。金属也被检测到：铝（92-218 µg/L）、铁（49-349 µg/L）、钾（160-3860 µg/L）、锰（3 µg/L）和钡（1-7 µg/L）。相关分析显示，6ppd -醌与HMMM之间存在显著正相关（r = 0.893,p = 0.000507），对乙酰氨基酚、咖啡因、可替宁和利多卡因之间存在显著正相关（r = 0.501-0.980,p <； 0.05），提示其共同来源可能是车辆活动频繁和废水外排。这些发现强调了干旱地区需要在极端天气事件期间实施有针对性的雨水管理和监测战略，以解决洪水驱动的环境污染物动员问题。

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引用次数: 0

Mesophilic compostability of polylactic acid and the associated microbiome as revealed by metagenomics 宏基因组学揭示的聚乳酸的中温堆肥性和相关微生物组

IF 8.1 Q1 ENGINEERING, ENVIRONMENTAL

Journal of hazardous materials letters

Pub Date : 2025-08-06 DOI: 10.1016/j.hazl.2025.100161

Shu Wei Hsueh , Anya Callista Kurniadi , Tan S.M. Amelia , Chin-Fa Lee , Sebastian D. Fugmann , Shu Yuan Yang

Polylactic acid (PLA), the most popular bioplastic, has high sustainability potential as it is bio-sourced and also harbors biodegradability. A form of its biodegradability is via composting, and it was previously established that thermophilic temperatures are needed for PLA breakdown in composts. Here we report the development of composts that have overcome the high-temperature requirement for PLA composting. Our mesophilic composts exhibited clear PLA biodegradability, and this is due to specific biological activity enriched in our material. To investigate the nature of this mesophilic activity, we conducted metagenomics analysis to reveal the microbial composition and enzyme-coding potential associated with PLA biodegradation. These efforts revealed multiple enzyme subtypes with strong enrichment on PLA surfaces in our trained composts, and the top candidate was a type of hydro-lyase, an enzyme that can cleave ester bonds in the absence of water. Hydro-lyases represent a novel class of enzymes that could facilitate PLA degradation, and our results point to the model that the combinatorial action of multiple types of enzymes is what drives PLA biodegradation and how the temperature barrier for PLA composting is overcome.

聚乳酸（PLA）是最受欢迎的生物塑料，由于其生物来源和生物降解性，具有很高的可持续性潜力。其生物可降解性的一种形式是通过堆肥，并且以前已经确定需要嗜热温度才能在堆肥中分解聚乳酸。在这里，我们报告了堆肥的发展，克服了高温要求的聚乳酸堆肥。我们的中温堆肥表现出明显的聚乳酸生物降解性，这是由于我们的材料中富含特定的生物活性。为了研究这种中温活性的性质，我们进行了宏基因组学分析，以揭示与聚乳酸生物降解相关的微生物组成和酶编码潜力。这些努力揭示了多种酶亚型在我们训练的堆肥中PLA表面上有很强的富集，其中最可能的是一种水解酶，一种在没有水的情况下可以裂解酯键的酶。水解酶代表了一类可以促进PLA降解的新型酶，我们的研究结果表明，多种酶的组合作用是驱动PLA生物降解的原因，以及如何克服PLA堆肥的温度障碍。

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引用次数: 0

A laser-enabled low carbon emission pyrometallurgical approach to recycle Li-ion batteries via silicothermic reductions 一种激光低碳排放的火法冶金方法，通过硅热还原回收锂离子电池

IF 8.1 Q1 ENGINEERING, ENVIRONMENTAL

Journal of hazardous materials letters

Pub Date : 2025-07-24 DOI: 10.1016/j.hazl.2025.100160

James Chen, Ruby Zhang, Maciej Podlesny, Tyler Smith, Chao Shi, Jian Li

In response to the growing shift from graphite to silicon in Li-ion battery anodes, we propose a novel low-carbon pyrometallurgical recycling method that uses silicon as the reducing agent. Silicon was chosen as the reductant because, as the emerging high-capacity anode material, it not only integrates seamlessly with next-generation battery chemistries but also offers a substantially lower carbon footprint than conventional carbon-based reducing agents. The thermodynamics and reaction mechanism between LiCoO₂ and Si are investigated using differential thermal and thermogravimetric analyses. The reaction products are characterized by scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray diffraction. When heated to 1500 °C, LiCoO₂ undergoes simultaneous decomposition and melting, reacting with Si to produce cobalt spheres. Through a laser-enabled recycling process for only 30 s with a laser power of 2 kW, LiCoO₂ is reduced via silicothermic reaction to a Co–Si alloy with only a small amount of slag (Li₂SiO₃ and Li₂Co(SiO₄)). This successful use of silicon paves the way for a cleaner, more sustainable battery recycling strategy.

为了应对锂离子电池负极从石墨到硅的日益转变，我们提出了一种新的低碳火法回收方法，该方法使用硅作为还原剂。之所以选择硅作为还原剂，是因为作为新兴的高容量阳极材料，它不仅与下一代电池化学物质无缝集成，而且比传统的碳基还原剂提供更低的碳足迹。利用差热分析和热重分析研究了LiCoO2与Si之间的热力学和反应机理。用扫描电子显微镜、能量色散x射线能谱和x射线衍射对反应产物进行了表征。当加热到1500 °C时，LiCoO2同时分解和熔化，与Si反应生成钴球。通过激光回收过程，激光功率为2 kW，时间仅为30 s， LiCoO2通过硅热反应还原为Co-Si合金，仅含有少量渣（Li2SiO3和Li2Co(SiO4)）。硅的成功使用为更清洁、更可持续的电池回收策略铺平了道路。

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引用次数: 0

Fungal proteomic response to PFAS mixtures: Defense or offense? 真菌对PFAS混合物的蛋白质组反应：防御还是进攻？

IF 8.1 Q1 ENGINEERING, ENVIRONMENTAL

Journal of hazardous materials letters

Pub Date : 2025-07-22 DOI: 10.1016/j.hazl.2025.100159

Kshitija Shah , Vijaya Pandey , Himadri Bose , Yun Hao , Rohan Ghosh Choudhuri , Allison Connolly , Hilary Wyner , Elizabeth Deyett , Kent Sorenson , James A. Wohlschlegel , Shaily Mahendra

The differential expression of molecular markers identified in response to environmental contaminants offer insights into early-stage resilience pathways that may support biological remediation approaches. Per- and polyfluoroalkyl substances (PFAS) are chemically stable, persistent environmental pollutants, which are associated with multiple adverse health effects. While fungi possess oxidative enzymes with potential for PFAS biotransformation, the molecular basis of their tolerance and response remains poorly understood. This study investigated the proteomic response of Phanerochaete chrysosporium to 10 mg/L PFOA and an environmentally relevant concentration of a PFAS mixture. Although no measurable PFAS degradation was observed over a 25-day exposure period, significant differential protein expression of key stress-response proteins such as cytochrome P450s, glutathione S-transferases, heat shock proteins, peroxidases, and ABC transporters were noted, in both intra- and extracellular fractions. Functional enrichment revealed the activation of pathways related to posttranslational modification, protein turnover, membrane efflux mechanisms, catabolism, and signal transduction. Proteomic profiles were shaped more closely by exposure duration and localization than by compound identity. These findings highlight the early-stage adaptations and signaling mechanisms of wood-decaying fungi under PFAS stress, which precede observable chemical breakdown, and offer critical insights into fungal responses that may be leveraged for future monitoring and bioremediation strategies.

在环境污染物响应中识别的分子标记的差异表达提供了对早期恢复途径的见解，可能支持生物修复方法。全氟烷基和多氟烷基物质（PFAS）是化学稳定的持久性环境污染物，与多种不利的健康影响有关。虽然真菌拥有具有PFAS生物转化潜力的氧化酶，但其耐受性和反应的分子基础仍然知之甚少。本研究研究了黄孢Phanerochaete chrysosporium对10 mg/L PFOA和环境相关浓度PFAS混合物的蛋白质组学响应。尽管在25天的暴露期内没有观察到可测量的PFAS降解，但在细胞内和细胞外组分中，关键应激反应蛋白（如细胞色素p450、谷胱甘肽s转移酶、热休克蛋白、过氧化物酶和ABC转运蛋白）的蛋白表达存在显著差异。功能富集揭示了与翻译后修饰、蛋白质周转、膜外排机制、分解代谢和信号转导相关的途径的激活。暴露时间和定位比化合物身份更能塑造蛋白质组学特征。这些发现强调了木材腐烂真菌在PFAS胁迫下的早期适应和信号机制，这是在可观察到的化学分解之前，并为真菌反应提供了重要的见解，可以用于未来的监测和生物修复策略。

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引用次数: 0

Development of a fluorophilic ion-exchange material with dual binding mechanism for solid-phase extraction of PFAS 具有双结合机制的亲氟离子交换材料固相萃取PFAS的研制

IF 6.6 Q1 ENGINEERING, ENVIRONMENTAL

Journal of hazardous materials letters

Pub Date : 2025-07-13 DOI: 10.1016/j.hazl.2025.100158

Johanna Freilinger , Jan O. Back , Raphael Plangger , Herwig Schottenberger , Christian W. Huck , Marco Rupprich , Rania Bakry

Per- and polyfluoroalkyl substances (PFAS) are persistent contaminants for which authorities worldwide have imposed limits on drinking water, groundwater and surface water. This has created challenges in PFAS detection, leading to an urgent need for reliable and selective solid-phase extraction (SPE) materials for PFAS analysis. In addressing this demand, we have tailored highly crosslinked copolymers containing 3-(1H,1H,2H,2H-perfluorooctyl)-1-vinylimidazolium chloride as a comonomer with ethylene dimethacrylate in various molar ratios. For ionic fluorosurfactants, these copolymers feature a dual binding mechanism that synergistically combines fluorophilic interactions and electrostatic attraction, enhancing selectivity and efficiency. The adsorption behavior of short- and long-chain PFAS and their recoveries were evaluated and compared to commercial SPE cartridges. Characterization revealed the highest ion-exchange capacity (412.7 ± 22 µeq g⁻¹) for a monomer-to-crosslinker ratio of 2:1. The dynamic adsorption capacities for various PFAS ranged from 15.2 to 306 g⁻¹. Recovery experiments consistently demonstrated high PFAS recoveries (98.8–121.6 %), while enrichment studies from wastewater confirmed its robustness in complex environmental matrices (recoveries: 90.8–99.2 %). Additionally, reusability experiments showed consistent recoveries over five cycles (recoveries: 90.34–108.0 %). The findings underscore the potential of this innovative polyelectrolyte as a selective, regenerable, and efficient alternative to conventional SPE materials, qualifying it as a superior candidate for PFAS analysis.

全氟烷基和多氟烷基物质（PFAS）是持久性污染物，世界各地的主管部门对饮用水、地下水和地表水实施了限制。这给PFAS检测带来了挑战，导致迫切需要可靠和选择性的固相萃取（SPE）材料进行PFAS分析。为了满足这一需求，我们定制了高度交联的共聚物，其中含有3-（1H,1H,2H，2H-全氟辛基）-1-乙烯基咪唑氯作为共聚体，以不同的摩尔比与二甲基丙烯酸乙烯酯。对于离子氟表面活性剂，这些共聚物具有双重结合机制，将亲氟相互作用和静电吸引协同结合，提高了选择性和效率。对短链PFAS和长链PFAS的吸附性能和回收率进行了评价，并与商用固相萃取筒进行了比较。表征表明，当单体与交联剂的比例为2:1时，离子交换容量最高（412.7 ± 22µeq g−1）。不同PFAS的动态吸附量为15.2 ~ 306 g−1。回收实验一致证明了PFAS的高回收率（98.8-121.6 %），而废水富集研究证实了其在复杂环境基质中的稳健性（回收率：90.8-99.2 %）。此外，可重用性实验表明，在5个循环中，回收率一致（回收率：90.34-108.0 %）。这些发现强调了这种创新的聚电解质作为传统SPE材料的选择性、可再生和高效替代品的潜力，使其成为PFAS分析的优越候选者。

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引用次数: 0

Micropellets of Pleurotus ostreatus for copper removal: Influence of nutritional conditions and laccase activity on adsorption 平菇微球除铜：营养条件和漆酶活性对吸附的影响

IF 6.6 Q1 ENGINEERING, ENVIRONMENTAL

Journal of hazardous materials letters

Pub Date : 2025-06-21 DOI: 10.1016/j.hazl.2025.100157

Dinay Eloisa Durán-Sequeda , Aldo Ibarra-Rondón , Pedro Fragoso-Castilla

Pleurotus ostreatus has emerged as a promising model for heavy metal bioremediation. Given the known role of laccases in copper oxidation, it was hypothesized that higher laccase activity would correlate with increased copper removal. This study evaluated how the composition of the culture medium influences copper adsorption and its relationship with copper-induced laccase activity in this fungus. Fungal pellets were produced in two different media, and their morphological characteristics, laccase activity, and copper removal capacity were evaluated. The results show that, contrary to expectations, pellets with more complex morphology and higher laccase activity exhibited a maximum copper loading capacity approximately six-fold lower than those produced in media with lower laccase activity. Analysis of the pellets by SEM-EDX, FTIR, and confocal microscopy revealed some physicochemical differences. Pellets with higher copper loading capacity had higher fluorescence suggesting more cell wall polysaccharide content, but lower laccase activity. These results offer a possible link between copper removal and copper-induced laccase activity associated with compositional medium for fungal culture. This finding represents a novel approach to designing and optimizing fungal biotechnological solutions in heavy metal bioremediation.

平菇是一种很有前途的重金属生物修复模式。鉴于漆酶在铜氧化中的已知作用，假设漆酶活性越高，铜的脱除率越高。本研究考察了培养基组成对铜吸附的影响及其与铜诱导漆酶活性的关系。在两种不同的培养基中制备真菌颗粒，并对其形态特征、漆酶活性和铜去除能力进行了评价。结果表明，与预期相反，具有更复杂形态和更高漆酶活性的颗粒的最大铜负载能力比在较低漆酶活性的培养基中生产的颗粒低约6倍。通过SEM-EDX， FTIR和共聚焦显微镜对颗粒进行分析，发现了一些物理化学差异。铜负载量越大，颗粒的荧光越强，表明细胞壁多糖含量越高，但漆酶活性越低。这些结果提供了铜去除和铜诱导漆酶活性与真菌培养组成介质之间的可能联系。这一发现代表了一种设计和优化重金属生物修复真菌生物技术解决方案的新方法。

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引用次数: 0

PFAS destruction using supercritical water oxidation (SCWO) at Peterson Space Force Base 在彼得森太空部队基地使用超临界水氧化（SCWO）破坏PFAS

IF 6.6 Q1 ENGINEERING, ENVIRONMENTAL

Journal of hazardous materials letters

Pub Date : 2025-06-20 DOI: 10.1016/j.hazl.2025.100156

Hallie Webb , Stephen Rosansky , Samer Mohamad Al-Dirani , Kavitha Dasu , Christopher G. Scheitlin , Jeff Davis , Leonardo Chiques

Per- and polyfluoroalkyl substances (PFAS) have become a top environmental concern for the military due to the prevalence of PFAS contamination from aqueous film-forming-foams (AFFFs) used to suppress fires in emergencies and training exercises. Supercritical water oxidation (SCWO) has emerged as a promising technology for the removal and destruction of PFAS in aqueous media. This project used the SCWO-based PFAS Annihilator® at Peterson Space Force Base to treat unconcentrated, 50 %, and 79 % concentrated PFAS-contaminated water onsite and demonstrate the efficacy of the technology. This study analyzed target PFAS, total organofluorine, and non-PFAS contaminants removed by the SCWO system. Here, 94.3 % of target PFAS in the unconcentrated influent, 99.2 % in the 50 % concentrated influent, and 99.7 % in the 79 % concentrated influent were destroyed, while removing > 95.5 % of total organofluorine, with limited byproducts in the aqueous and vapor effluent. The mass balance achieved 52–102 % fluoride recovery across the three concentrations. A novel cost assessment demonstrates improved economic efficiency with increasing influent concentration, suggesting that SCWO can efficiently remove and destroy PFAS from contaminated water to levels below regulatory requirements, solidifying itself as an innovative solution to PFAS contamination.

全氟烷基和多氟烷基物质（PFAS）已成为军方最关心的环境问题，因为在紧急情况和训练演习中用于灭火的水性成膜泡沫（afff）普遍存在PFAS污染。超临界水氧化（SCWO）是一种很有前途的去除和破坏水介质中PFAS的技术。该项目在彼得森太空部队基地使用基于scwo的PFAS湮灭器®，现场处理未浓缩、50% %和79% %浓缩的PFAS污染水，并证明了该技术的有效性。本研究分析了SCWO系统去除的目标PFAS、总有机氟和非PFAS污染物。在这里，未浓缩进水中94.3 %的目标PFAS被破坏，在50% %的浓缩进水中99.2 %，在79 %的浓缩进水中99.7 %，同时去除>； 95.5 %的总有机氟，在水和蒸汽出水中产生有限的副产物。三种浓度下的氟化物回收率达到52-102 %。一项新的成本评估表明，随着进水浓度的增加，经济效率也会提高，这表明SCWO可以有效地去除和破坏受污染水中的PFAS，使其低于监管要求，从而巩固了其作为PFAS污染的创新解决方案的地位。

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引用次数: 0

Arsenic-induced phytotoxicity in Trigonella foenum-graecum and its regulation by thiol metabolism and ROS quenching enzymes 砷诱导的青翠Trigonella foenum-graecum的植物毒性及其硫醇代谢和ROS猝灭酶的调控

IF 6.6 Q1 ENGINEERING, ENVIRONMENTAL

Journal of hazardous materials letters

Pub Date : 2025-06-18 DOI: 10.1016/j.hazl.2025.100153

Javed Ahmad , Mohammad Affan Baig , Arlene Asthana Ali , Md. Amjad Beg , Asma A. Al-Huqail , Faheema Khan , Malik Zainul Abdin , Mohammad Irfan Qureshi

Arsenic contamination of soils and groundwater affects nearly 106 countries, exposing an estimated 230 million people worldwide to a range of health risks including cancer, cardiovascular disease, diabetes, etc. It also poses significant risks to plants such as inhibited growth, reduced crop yields, and soil health degradation. This study investigates the arsenic (As)-induced changes in dynamics of proteome, sulfur metabolism, antioxidant enzymes and changes in stress tolerance mechanisms in Trigonella foenum-graecum under different concentrations. Hydroponically grown 30-day-old plants were exposed to 0, 25, 50, 75, and 100 µM As for 10 days. The results showed increased oxidative stress and reduced growth at higher As concentrations. Enzymes related to thiol metabolism, including ATP sulfurylase and serine acetyltransferase, were significantly upregulated at 50 and 100 µM As, alongside increased cysteine and glutathione content linked to the upregulation of S-deficiency-induced 2 isoform X2 protein. Antioxidant enzymes (SOD, APX, CAT, GR, GPX, and GST) also exhibited enhanced activity. Proteomic analysis revealed 46 differentially expressed protein spots, including proteins involved in growth and photosynthesis, such as gibberellin 20-oxidase and RuBisCO. Defence proteins like trehalose phosphate phosphatase, calmodulin, and pectinesterase were upregulated, aiding stress tolerance. Sulfur metabolism proteins, such as glutathione S-transferase, were activated to counteract oxidative stress. Metallothioneins (MTs) were notably upregulated, contributing to arsenic detoxification. Transport proteins, including ABC transporters and ATP synthase beta subunits seems to play important roles in arsenic resistance. Additionally, proteins involved in protein degradation and redox balance, such as the RING finger protein and selenoprotein W1, supported the plant's adaptive response. These findings highlight the complex proteomic changes that enable T. foenum-graecum to tolerate arsenic stress and enhance its phytoremediation potential.

土壤和地下水的砷污染影响到近106个国家，使全世界约2.3亿人面临一系列健康风险，包括癌症、心血管疾病、糖尿病等。它还对植物构成重大风险，如抑制生长、降低作物产量和土壤健康退化。本研究探讨了砷（As）对不同浓度下葫芦巴（Trigonella foenum-graecum）蛋白质组学、硫代谢、抗氧化酶动力学的影响以及胁迫耐受机制的变化。水培生长的30日龄植物分别暴露于0、25、50、75和100 µM As中10天。结果表明，高浓度砷增加了氧化应激，降低了生长。与硫醇代谢相关的酶，包括ATP硫酰化酶和丝氨酸乙酰转移酶，在50和100 µM As时显著上调，同时增加的半胱氨酸和谷胱甘肽含量与s缺陷诱导的2异构体X2蛋白上调有关。抗氧化酶（SOD、APX、CAT、GR、GPX和GST）的活性也有所增强。蛋白质组学分析发现了46个差异表达的蛋白点，包括与生长和光合作用有关的蛋白质，如赤霉素20氧化酶和RuBisCO。防御蛋白，如海藻糖磷酸酶、钙调蛋白和果胶酯酶被上调，有助于抗逆性。硫代谢蛋白，如谷胱甘肽s -转移酶，被激活以对抗氧化应激。金属硫蛋白（MTs）显著上调，参与砷解毒。转运蛋白，包括ABC转运蛋白和ATP合成酶β亚基似乎在砷抗性中起重要作用。此外，参与蛋白质降解和氧化还原平衡的蛋白质，如环指蛋白和硒蛋白W1，支持了植物的适应性反应。这些发现强调了复杂的蛋白质组学变化，使T. foenum-graecum能够耐受砷胁迫并增强其植物修复潜力。

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引用次数: 0

Sulfotransferases in mediating the chlorophenol metabolism: Metabolites identification and metabolic characterization 介导氯酚代谢的硫转移酶：代谢物鉴定和代谢特性

IF 6.6 Q1 ENGINEERING, ENVIRONMENTAL

Journal of hazardous materials letters

Pub Date : 2025-06-18 DOI: 10.1016/j.hazl.2025.100155

Kai Yang , Guoqiang qin , RuoYong Jia , Haoqian Zhang , Qiangqiang Gan , Shao-You Lu , Jian-Jun Liu , Zhong-Ze Fang

Chlorophenols (CPs) are persistent organic pollutants (POPs) widely detected in the natural environment. Sulfotransferases are a group of crucial phase II metabolic enzyme involved in the metabolism of endogenous and exogenous substances. This research aims to identify the metabolite and characterize the specific isoforms involved in the sulfonation of each CP. Recombinant enzyme assays and chemical inhibitors were used to characterize the primary SULT isoforms involved in the sulfonation of each CP. Our findings indicated that mono-sulfonate was identified in each incubation system. SULT1A1 predominated the sulfonation of monochlorophenol (MCP), SULT1A1, SULT1B1, and SULT1E1 emerged as the pivotal SULT isoforms mediating the sulfonation of 2,4-dichlorophenol (2,4-DCP), trichlorophenol (2,3,4-TCP), 2,4,6-trichlorophenol (2,4,6-TCP), and 3,4,5-trichlorophenol (3,4,5-TCP). Additionally, sulfonation of 2,4,5-trichlorophenol (2,4,5-TCP), 2,3,4,5-tetrachlorophenol (2,3,4,5-TECP), and 2,3,4,6-tetrachlorophenol (2,3,4,6-TECP) were primarily catalyzed by SULT1B1. Furthermore, SULT1B1 and SULT2A1 were the major isoforms involved in the sulfonation of 2,3,5,6-tetrachlorophenol (2,3,5,6-TECP) and pentachlorophenol (PCP). These results provide important insights into the metabolic elimination and toxicity of CPs from a novel perspective.

氯酚是一种广泛存在于自然环境中的持久性有机污染物。硫转移酶是一类重要的II期代谢酶，参与内源性和外源性物质的代谢。本研究旨在鉴定代谢物并表征参与每种CP磺化的特定亚型。重组酶测定和化学抑制剂用于表征参与每种CP磺化的主要SULT亚型。我们的研究结果表明，在每种培养体系中都鉴定出单磺酸盐。SULT1A1主导了单氯苯酚（MCP）的磺化，SULT1A1、SULT1B1和SULT1E1是SULT的关键同工异构体，介导了2,4-二氯苯酚（2,4- dcp）、三氯苯酚（2,3,4- tcp）、2,4,6-三氯苯酚（2,4,6- tcp）和3,4,5-三氯苯酚（3,4,5- tcp）的磺化。此外，2,4,5-三氯苯酚（2,4,5- tcp）、2,3,4,5-四氯苯酚（2,3,4,5- tecp）和2,3,4,6-四氯苯酚（2,3,4,6- tecp）的磺化反应主要由SULT1B1催化。此外，SULT1B1和SULT2A1是参与2,3,5,6-四氯酚（2,3,5,6- tecp）和五氯酚（PCP）磺化的主要亚型。这些结果为CPs的代谢消除和毒性提供了一个新的视角。

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引用次数: 0

A dual approach using UV irradiation and subcritical water extraction for enhanced PLA waste degradation in a bioaugmented food composter 采用紫外照射和亚临界水萃取的双重方法在生物增强食物堆肥中增强聚乳酸废物的降解

IF 6.6 Q1 ENGINEERING, ENVIRONMENTAL

Journal of hazardous materials letters

Pub Date : 2025-06-05 DOI: 10.1016/j.hazl.2025.100154

Christian Adi Pratama , Avnish Nitin Mistry , Saowaluk Krainara , Patamavadee Treeson , Nattapong Tuntiwiwattanapun , Nichakorn Khondee , Luthfia Dwi Rachmani , Ekawan Luepromchai

Polylactic acid (PLA) ranks among the most extensively produced bioplastics, raising waste management concerns globally. This study explored dual pretreatment strategies, specifically photolysis by Ultraviolet-C (UVC) and hydrolysis by subcritical water extraction (SWE), for PLA waste treatment. UVC irradiation reduced the mechanical strength and viscosity average molecular weight (Mv) of PLA, but the effects were more significant in thin PLA films than in thick PLA beverage cups. Thus, the PLA cups were selected for SWE treatment, which reduced their Mv from 190,000 to 5300. The pulverized SWE-treated PLA was later used for inducing protease and esterase in PLA-degrading bacterial consortium EAc. This active inoculum was applied as a bioaugmentation agent in a food composter for degrading UVC-treated PLA cups with food waste. PLA weight loss in the bioaugmented food composter (42 %) after 56 days was greater than the naturally attenuated composter (1 %). The residual PLA in the final bioaugmented compost had a relatively smaller size and lower Mv with prominent surface erosion. In addition, the 16S rRNA gene amplicon sequencing revealed that the bioaugmentation promoted bacterial diversity and community interactions. Conclusively, PLA waste can be treated by UVC irradiation followed by composting with food waste using an active EAc inoculum.

聚乳酸（PLA）是生产最广泛的生物塑料之一，引起了全球对废物管理的关注。本研究探索了双预处理策略，即紫外- c光解（UVC）和亚临界水萃取（SWE）水解处理PLA废物。UVC辐照降低了聚乳酸的机械强度和粘度平均分子量（Mv），但对聚乳酸薄膜的影响比对聚乳酸饮料杯的影响更显著。因此，选择PLA杯进行SWE处理，将其Mv从190,000降低到5300。将经sw处理的聚乳酸粉碎后，在聚乳酸降解细菌联合体EAc中诱导蛋白酶和酯酶。该活性接种物作为生物增强剂应用于食物堆肥机中，用于降解uvc处理的聚乳酸杯和食物垃圾。生物增强食物堆肥56 d后PLA失重（42. %）大于自然减重（1 %）。最终生物增强堆肥中残余聚乳酸体积相对较小，Mv较低，表面侵蚀明显。此外，16S rRNA基因扩增子测序显示，生物增强促进了细菌多样性和群落相互作用。综上所述，聚乳酸废物可以通过UVC照射处理，然后使用活性EAc接种物与食物垃圾一起堆肥。

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引用次数: 0