Pub Date : 2023-01-01DOI: 10.21608/besps.2022.147058.1126
H. Salem, Mona A. Kora, S. Khodir
{"title":"The Potential Antistress and Gastroprotective Effects of Berberine in Immobilization Stress Induced Gastric Ulcer in Rats","authors":"H. Salem, Mona A. Kora, S. Khodir","doi":"10.21608/besps.2022.147058.1126","DOIUrl":"https://doi.org/10.21608/besps.2022.147058.1126","url":null,"abstract":"","PeriodicalId":9347,"journal":{"name":"Bulletin of Egyptian Society for Physiological Sciences","volume":"179 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78148131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.21608/besps.2022.152592.1127
Abdelhamid Abouelmagd, Ghida Hassan
{"title":"Hormonal and Metabolic Benefits of FlaxSeed Oil in Ovariectomized Rats","authors":"Abdelhamid Abouelmagd, Ghida Hassan","doi":"10.21608/besps.2022.152592.1127","DOIUrl":"https://doi.org/10.21608/besps.2022.152592.1127","url":null,"abstract":"","PeriodicalId":9347,"journal":{"name":"Bulletin of Egyptian Society for Physiological Sciences","volume":"87 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83422434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-13DOI: 10.21608/besps.2022.176884.1132
Gehan M. Soliman, Nermine Beshara, Hala Abd Elgawad, Eman Othman Abd Elrahman, Doaa A. Abd Elmonsif, M. Kampschulte
{"title":"Comparative study of combined treatment with systemic substance P and local stromal derived factor 1 alpha on recombinant human bone morphogenic protein 2- induced ectopic bone formation in a rat model.","authors":"Gehan M. Soliman, Nermine Beshara, Hala Abd Elgawad, Eman Othman Abd Elrahman, Doaa A. Abd Elmonsif, M. Kampschulte","doi":"10.21608/besps.2022.176884.1132","DOIUrl":"https://doi.org/10.21608/besps.2022.176884.1132","url":null,"abstract":"","PeriodicalId":9347,"journal":{"name":"Bulletin of Egyptian Society for Physiological Sciences","volume":"107 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73356290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.21608/besps.2022.122545.1121
Ghida Hassan
Background/ Aim: Several studies suggested that beta-glucan may have a cardioprotective role against developing high blood pressure (BP). The current study investigated the underlying mechanism of beta glycan as antihypertensive and its effect on vascular functions in males. Materials & Methods: A total of 20 adult male Wistar rats weighing 180-200 g were divided into 2 groups 10 per each. Group I : Control group were fed standard rat chow for 15 weeks. Group II: Beta glycan fed group in the form of oat cereals by gavage(305 mg per kg body weight per day) for a period of 15 weeks. Rats of both groups were subjected to blood pressure measurement at 0, 10, 15 weeks. On the day of sacrifice, blood samples were collected for measurement of plasma catalase, plasma MDA & lipid profile. Aortic tissue was excised to study vascular reactivity and measurement of aortic tissue nitrate. Results: Significant reduction in the systolic & diastolic blood pressure in beta glycan fed group. This was accompanied by significant increase in plasma catalase & aortic tissue nitrate while plasma MDA, TC, TAG , LDL & atherogenic index were significantly decreased. Significant decrease in the contractile response of aorta to phenylephrine with significant increase in the relaxing response to acetylcholine in the beta glycan fed ones. In Conclusion : Beta glycan is a beneficial antihypertensive vasorelaxing agent that is recommended to be added to the diet especially in males to balance the proatherogenic effect of testosterone. This cardioprotective role is achieved through decreasing the oxidative stress, triglycerides and total cholesterol level. Inaddition, the aortic tissue nitrate was significantly increased which is considered to be a powerful vasodilator agent. stress hyperlipidemia
{"title":"Study of the effect of beta glycan supplemented diet on vascular functions in male rats","authors":"Ghida Hassan","doi":"10.21608/besps.2022.122545.1121","DOIUrl":"https://doi.org/10.21608/besps.2022.122545.1121","url":null,"abstract":"Background/ Aim: Several studies suggested that beta-glucan may have a cardioprotective role against developing high blood pressure (BP). The current study investigated the underlying mechanism of beta glycan as antihypertensive and its effect on vascular functions in males. Materials & Methods: A total of 20 adult male Wistar rats weighing 180-200 g were divided into 2 groups 10 per each. Group I : Control group were fed standard rat chow for 15 weeks. Group II: Beta glycan fed group in the form of oat cereals by gavage(305 mg per kg body weight per day) for a period of 15 weeks. Rats of both groups were subjected to blood pressure measurement at 0, 10, 15 weeks. On the day of sacrifice, blood samples were collected for measurement of plasma catalase, plasma MDA & lipid profile. Aortic tissue was excised to study vascular reactivity and measurement of aortic tissue nitrate. Results: Significant reduction in the systolic & diastolic blood pressure in beta glycan fed group. This was accompanied by significant increase in plasma catalase & aortic tissue nitrate while plasma MDA, TC, TAG , LDL & atherogenic index were significantly decreased. Significant decrease in the contractile response of aorta to phenylephrine with significant increase in the relaxing response to acetylcholine in the beta glycan fed ones. In Conclusion : Beta glycan is a beneficial antihypertensive vasorelaxing agent that is recommended to be added to the diet especially in males to balance the proatherogenic effect of testosterone. This cardioprotective role is achieved through decreasing the oxidative stress, triglycerides and total cholesterol level. Inaddition, the aortic tissue nitrate was significantly increased which is considered to be a powerful vasodilator agent. stress hyperlipidemia","PeriodicalId":9347,"journal":{"name":"Bulletin of Egyptian Society for Physiological Sciences","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73636467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.21608/besps.2022.130999.1122
F. Moustafa, M. Abdel-Fadeil, Salah Atta, Azza S Abdelhaffez
Background: Circulating remodeling markers such as Galectin-3 (Gal-3) are significantly higher in atrial fibrillation (AF) patients. Nevertheless, there is still scarcity in the published literature regarding Gal-3 levels and risk of stroke in AF patients. The aim of this study was to evaluate the correlation of Gal-3 blood levels with the predicted thromboembolic risk factors according to the current standard scoring system (CHA2DS2-VASc) as well as some other echocardiographic and laboratory parameters, among patients with paroxysmal or persistent non-valvular AF. Methods: Three groups were enrolled in this cross-sectional study: low (70 patients), moderate (50 patients) and high risk (40 patients) categorized according to CHA2DS2-VASc score. Serum concentrations of Gal-3 were detected by an enzyme-linked immunosorbent assay (ELISA). In addition, activated partial thromboplastin time (aPTT) was estimated in all patients. Echocardiography was carried out to evaluate valvular functions and left ventricular ejection fraction and left atrial volume index (LAVI). Results: Patients with high risk had significantly higher Gal-3 level, lower aPTT duration and higher LAVI values than moderate and low risk. There were statistically significant positive correlations between CHA2DS2-VASc score and serum Gal-3 level and LAVI. Similarly, there was statistically significant negative correlation between CHA2DS2-VASc score and aPTT. Female patients at all age groups had significantly higher Gal-3 levels than male patients. Conclusion: Serum Gal-3 level might be a predictor for thromboembolic complication in AF patients with potential clinical benefit.
{"title":"Galectin-3 as a predictor for thromboembolic risk in patients with non-valvular atrial fibrillation assessed by CHA2DS2-VASc scoring","authors":"F. Moustafa, M. Abdel-Fadeil, Salah Atta, Azza S Abdelhaffez","doi":"10.21608/besps.2022.130999.1122","DOIUrl":"https://doi.org/10.21608/besps.2022.130999.1122","url":null,"abstract":"Background: Circulating remodeling markers such as Galectin-3 (Gal-3) are significantly higher in atrial fibrillation (AF) patients. Nevertheless, there is still scarcity in the published literature regarding Gal-3 levels and risk of stroke in AF patients. The aim of this study was to evaluate the correlation of Gal-3 blood levels with the predicted thromboembolic risk factors according to the current standard scoring system (CHA2DS2-VASc) as well as some other echocardiographic and laboratory parameters, among patients with paroxysmal or persistent non-valvular AF. Methods: Three groups were enrolled in this cross-sectional study: low (70 patients), moderate (50 patients) and high risk (40 patients) categorized according to CHA2DS2-VASc score. Serum concentrations of Gal-3 were detected by an enzyme-linked immunosorbent assay (ELISA). In addition, activated partial thromboplastin time (aPTT) was estimated in all patients. Echocardiography was carried out to evaluate valvular functions and left ventricular ejection fraction and left atrial volume index (LAVI). Results: Patients with high risk had significantly higher Gal-3 level, lower aPTT duration and higher LAVI values than moderate and low risk. There were statistically significant positive correlations between CHA2DS2-VASc score and serum Gal-3 level and LAVI. Similarly, there was statistically significant negative correlation between CHA2DS2-VASc score and aPTT. Female patients at all age groups had significantly higher Gal-3 levels than male patients. Conclusion: Serum Gal-3 level might be a predictor for thromboembolic complication in AF patients with potential clinical benefit.","PeriodicalId":9347,"journal":{"name":"Bulletin of Egyptian Society for Physiological Sciences","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83384510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.21608/besps.2022.121674.1119
M. Motawee, F. Hassan, Ahmed A. Damanhory, Passant M. Mohie, M. Khalifa, Yasser M. Elbastawisy
{"title":"Possible Protective Effect of Each of Omega-3 PUFA and Leptin on Indomethacin-Induced Gastric Ulcer in Rats with type II DM","authors":"M. Motawee, F. Hassan, Ahmed A. Damanhory, Passant M. Mohie, M. Khalifa, Yasser M. Elbastawisy","doi":"10.21608/besps.2022.121674.1119","DOIUrl":"https://doi.org/10.21608/besps.2022.121674.1119","url":null,"abstract":"","PeriodicalId":9347,"journal":{"name":"Bulletin of Egyptian Society for Physiological Sciences","volume":"221 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76625981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.21608/besps.2022.142802.1125
S. El-Beah, D. Shaalan, Adel Fathi, S. Yussif
Background: As being the third‑most common cancer type in males and the second‑most common cancer type in females worldwide, the incidence of colorectal carcinoma is increasing and associated with poor prognosis. Programmed cell death protein 1 (PD-1) gene encodes an inhibitory cell surface receptor that is thought to be implicated in influencing the cancer cells’ evasion of the host immune system. The current work was conducted to evaluate the association of PD-1 single nucleotide polymorphisms, PD-1.3G/A (rs11568821) and PD-1.5 C/T (rs2227981) haplotypes with both the susceptibility and the onset of colorectal cancer in the Egyptian population. Methods and Results: The PD-1.3 G/A and, PD-1.5 C/T polymorphisms were investigated in 100 colorectal cancer patients and 100 healthy controls by allelic discrimination technique through TaqMan real-time polymerase chain reaction. The lowest overall risk of colorectal cancer was associated with the haplotype G/T while the A/T haplotype showed the highest risk among the four being insignificant. However, the A/T haplotype was significantly associated with an increased risk of the early onset disease by 4.4 times as compared to the reference haplotype G/C. Moreover, the PD1.5C/T SNP was in a significant linkage disequilibrium with PD1.3G/A SNP (D = 0.0255, D'= 0.151, r= 0.1298 and p = 0.0094). Conclusion: Among the Egyptian population, the G/T haplotype was considered protective acquiring the least risk for colorectal cancer, while the mutant A/T haplotype was not only associated with a higher risk of the disease but also with a significantly higher risk to exhibit early onset colorectal cancer.
{"title":"Haplotyping of PD-1 Polymorphisms in Egyptian Patients with Colorectal Cancer: A Case-Control Study.","authors":"S. El-Beah, D. Shaalan, Adel Fathi, S. Yussif","doi":"10.21608/besps.2022.142802.1125","DOIUrl":"https://doi.org/10.21608/besps.2022.142802.1125","url":null,"abstract":"Background: As being the third‑most common cancer type in males and the second‑most common cancer type in females worldwide, the incidence of colorectal carcinoma is increasing and associated with poor prognosis. Programmed cell death protein 1 (PD-1) gene encodes an inhibitory cell surface receptor that is thought to be implicated in influencing the cancer cells’ evasion of the host immune system. The current work was conducted to evaluate the association of PD-1 single nucleotide polymorphisms, PD-1.3G/A (rs11568821) and PD-1.5 C/T (rs2227981) haplotypes with both the susceptibility and the onset of colorectal cancer in the Egyptian population. Methods and Results: The PD-1.3 G/A and, PD-1.5 C/T polymorphisms were investigated in 100 colorectal cancer patients and 100 healthy controls by allelic discrimination technique through TaqMan real-time polymerase chain reaction. The lowest overall risk of colorectal cancer was associated with the haplotype G/T while the A/T haplotype showed the highest risk among the four being insignificant. However, the A/T haplotype was significantly associated with an increased risk of the early onset disease by 4.4 times as compared to the reference haplotype G/C. Moreover, the PD1.5C/T SNP was in a significant linkage disequilibrium with PD1.3G/A SNP (D = 0.0255, D'= 0.151, r= 0.1298 and p = 0.0094). Conclusion: Among the Egyptian population, the G/T haplotype was considered protective acquiring the least risk for colorectal cancer, while the mutant A/T haplotype was not only associated with a higher risk of the disease but also with a significantly higher risk to exhibit early onset colorectal cancer.","PeriodicalId":9347,"journal":{"name":"Bulletin of Egyptian Society for Physiological Sciences","volume":"64 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77375148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.21608/besps.2022.135210.1123
N. Elmongy, I. Hussein, W. Ahmed, Ibrahim M. Shatla
Background : As a result of the increased consumption of sugar-rich and fatty-products, and the increase in the preference for such products, metabolic disorders are becoming more common at a younger age. Hyperlipidemia, insulin resistance [IR] and inflammatory status induced by high fat high fructose diet [HFHFD] increase the risk of developing cardiovascular diseases [CVDs] which are the leading cause of death worldwide. Objective : This study is aiming to determine the effects of HFHFD on cardiovascular disease [CVD]-related parameters. Also, the possible protective and curative effects of cinnamon extract on cardiac diseases. Methodology : Fifty adult male Sprague Dawley rats were divided into five groups: Group I: [control]. Group II rats were fed a diet with 45 kcal% fat and drink fructose 60% [HFHFD] for 8w. Group III: fed on HFHFD and pioglitazone [PGZ 20 mg/kg/b.wt]. Group IV: fed on HFHFD and cinnamon extract200mg/kg.b.w. Group V: fed on HFHFD and treated with combination of PGZ drug and cinnamon extract. At the end of the experiment, blood glucose, serum insulin, lipid profile, and oxidative stress markers were done. Heart tissues were used for Inflammatory cytokines [tumor necrosis factor-α [TNF-α] and interleukin 6 [IL-6], nuclear factor kappa [NF-κB], and cardiac enzymes beside histopathological examination. Results: HFHFD administration resulted signs of cardiomyopathy revealed by significant increase in blood glucose, serum insulin, homeostasis model assessment of IR [HOMA- IR] index with disturbed lipid profile. Also, significant increase in serum cardiac enzymes, inflammatory cytokines and oxidative stress markers were also noticed. PGZ and cinnamon extract treatment resulted in a significant decrease in glucose, insulin, and HOMA- IR index, amelioration of lipid profile, cardiac enzymes, inflammatory cytokines and oxidative stress markers. Conclusion : The current study revealed that cinnamon extract improves cardiomyopathy resulted from HFHFD administration possibly via hypolipidemic, anti-inflammatory and anti-oxidative ways. It can be used as food supplement has cardioprotective role in patients with metabolic disorders
{"title":"Cardioprotective effect of Cinnamomum zeylanicum extract on rats fed on high fat high fructose diet","authors":"N. Elmongy, I. Hussein, W. Ahmed, Ibrahim M. Shatla","doi":"10.21608/besps.2022.135210.1123","DOIUrl":"https://doi.org/10.21608/besps.2022.135210.1123","url":null,"abstract":"Background : As a result of the increased consumption of sugar-rich and fatty-products, and the increase in the preference for such products, metabolic disorders are becoming more common at a younger age. Hyperlipidemia, insulin resistance [IR] and inflammatory status induced by high fat high fructose diet [HFHFD] increase the risk of developing cardiovascular diseases [CVDs] which are the leading cause of death worldwide. Objective : This study is aiming to determine the effects of HFHFD on cardiovascular disease [CVD]-related parameters. Also, the possible protective and curative effects of cinnamon extract on cardiac diseases. Methodology : Fifty adult male Sprague Dawley rats were divided into five groups: Group I: [control]. Group II rats were fed a diet with 45 kcal% fat and drink fructose 60% [HFHFD] for 8w. Group III: fed on HFHFD and pioglitazone [PGZ 20 mg/kg/b.wt]. Group IV: fed on HFHFD and cinnamon extract200mg/kg.b.w. Group V: fed on HFHFD and treated with combination of PGZ drug and cinnamon extract. At the end of the experiment, blood glucose, serum insulin, lipid profile, and oxidative stress markers were done. Heart tissues were used for Inflammatory cytokines [tumor necrosis factor-α [TNF-α] and interleukin 6 [IL-6], nuclear factor kappa [NF-κB], and cardiac enzymes beside histopathological examination. Results: HFHFD administration resulted signs of cardiomyopathy revealed by significant increase in blood glucose, serum insulin, homeostasis model assessment of IR [HOMA- IR] index with disturbed lipid profile. Also, significant increase in serum cardiac enzymes, inflammatory cytokines and oxidative stress markers were also noticed. PGZ and cinnamon extract treatment resulted in a significant decrease in glucose, insulin, and HOMA- IR index, amelioration of lipid profile, cardiac enzymes, inflammatory cytokines and oxidative stress markers. Conclusion : The current study revealed that cinnamon extract improves cardiomyopathy resulted from HFHFD administration possibly via hypolipidemic, anti-inflammatory and anti-oxidative ways. It can be used as food supplement has cardioprotective role in patients with metabolic disorders","PeriodicalId":9347,"journal":{"name":"Bulletin of Egyptian Society for Physiological Sciences","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78928553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-01DOI: 10.21608/besps.2022.118340.1116
Elham Nasif, R. Shalaby, Sarah Abd El -Khalik, rasha Abd Ellatif
Background: Gastric ulcer is one of the most ubiquitous gastrointestinal tract disorders, causing high morbidity. The goal of this study was to explore at the mechanistic effects of Baicalein (BA) and/or Empagliflozin (EMP) treatment on gastric ulcer caused by indomethacin and prednisolone, with an emphasis on the role of the HO-1/SIRT1 / HMGB1 signaling pathway.Material and methods: Fifty adult albino rats were allocated into 5 equal groups: control, ulcer (Prednisolone 10mg/kg for 6 days followed by indomethacin as single oral dose 30 mg/kg), BA (30 mg/kg), EMP (10 mg/kg), and BA + EMP groups. The volume of gastric juice, pH, free and total acidity was estimated. The gene expression of HO-1 and SIRT1 in gastric tissues was assessed by qRT PCR. Biochemical analysis of gastric tissues homogenates including HMGB-1, PGE2 & nitrites levels was performed. Assay of inflammatory markers and redox status were detected. Additionally, histological, scanning electron microscopic and immunohistochemical analyses were determined.Results: After 4 weeks of treatment, there was remarkable improvement of the histological architecture of rat gastric tissues. Upregulation of HO-1 and SIRT1 gene expression, as well as a decrease in HMGB1 level, resulted in improved inflammatory and oxidative stress biomarkers. Furthermore, immunohistochemical analysis revealed increase in Bcl-2 expression and decreased expression of Bax in the treated groups. Conclusion: Concurrent usage of BA & EMP against gastric ulcer in rats could be related to the interaction of their anti-oxidant, antiinflammatory, and anti-apoptotic activities via modulation of HO-1/SIRT1 / HMGB1 signaling pathway.. Keywords
{"title":"The Potential Anti-ulcerogenic Effects of Baicalein and/or Empagliflozin in Induced Gastric Ulcer in Rats: Modulating HO-1/SIRT1 / HMGB1 signaling Pathway","authors":"Elham Nasif, R. Shalaby, Sarah Abd El -Khalik, rasha Abd Ellatif","doi":"10.21608/besps.2022.118340.1116","DOIUrl":"https://doi.org/10.21608/besps.2022.118340.1116","url":null,"abstract":"Background: Gastric ulcer is one of the most ubiquitous gastrointestinal tract disorders, causing high morbidity. The goal of this study was to explore at the mechanistic effects of Baicalein (BA) and/or Empagliflozin (EMP) treatment on gastric ulcer caused by indomethacin and prednisolone, with an emphasis on the role of the HO-1/SIRT1 / HMGB1 signaling pathway.Material and methods: Fifty adult albino rats were allocated into 5 equal groups: control, ulcer (Prednisolone 10mg/kg for 6 days followed by indomethacin as single oral dose 30 mg/kg), BA (30 mg/kg), EMP (10 mg/kg), and BA + EMP groups. The volume of gastric juice, pH, free and total acidity was estimated. The gene expression of HO-1 and SIRT1 in gastric tissues was assessed by qRT PCR. Biochemical analysis of gastric tissues homogenates including HMGB-1, PGE2 & nitrites levels was performed. Assay of inflammatory markers and redox status were detected. Additionally, histological, scanning electron microscopic and immunohistochemical analyses were determined.Results: After 4 weeks of treatment, there was remarkable improvement of the histological architecture of rat gastric tissues. Upregulation of HO-1 and SIRT1 gene expression, as well as a decrease in HMGB1 level, resulted in improved inflammatory and oxidative stress biomarkers. Furthermore, immunohistochemical analysis revealed increase in Bcl-2 expression and decreased expression of Bax in the treated groups. Conclusion: Concurrent usage of BA & EMP against gastric ulcer in rats could be related to the interaction of their anti-oxidant, antiinflammatory, and anti-apoptotic activities via modulation of HO-1/SIRT1 / HMGB1 signaling pathway.. Keywords","PeriodicalId":9347,"journal":{"name":"Bulletin of Egyptian Society for Physiological Sciences","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76778213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-01DOI: 10.21608/besps.2022.120408.1118
Reda Abo- Elsoud, Amany Elfakhrany, Anwaar Shaban, A. Gaafar
Background: Risk of vascular hemodynamic impairment increases with age. So, finding a new therapeutic approach to counter the age-related vascular complications has been a challenge. Hence, we evaluated the therapeutic effects of fenofibrate (FEN) in ageing. We also studied the FEN anti-ageing effects, and the possible molecular mechanisms that may clarify these effects. Methods: Thirty male rats were equally divided into three groups: 1Control group: Received (1% CMC) via gastric lavage, 2aged non treated group: Received (1% CMC) via gastric lavage, and 3Aged (FEN) treated group: received FEN (30 mg/kg) dissolved in (1% CMC) via gastric lavage for 7 weeks. At the end of study, serum lipid profile, inflammatory and oxidative stress markers were performed. Lastly, rats were subjected for measurement of invasive mean arterial blood pressure (MABP) and vascular reactivity to NE and Ach. Results: Aging resulted in dyslipidemia and elevation of inflammatory biomarkers, with altered oxidative stress markers. Increased MABP and decreased vascular reactivity to NE and Ach were also notice. FEN restored significantly aged related biochemical and vascular pathophysiological changes. Conclusion: FEN could be a good candidate for cardiovascular deficits due to ageing via amelioration of dyslipidemia, inflammation, attenuation of oxidative stress, reduction of MABP and improvement of vascular reactivity.
{"title":"Effect of fenofibrate on biochemical and vascular reactivity changes in aged rats","authors":"Reda Abo- Elsoud, Amany Elfakhrany, Anwaar Shaban, A. Gaafar","doi":"10.21608/besps.2022.120408.1118","DOIUrl":"https://doi.org/10.21608/besps.2022.120408.1118","url":null,"abstract":"Background: Risk of vascular hemodynamic impairment increases with age. So, finding a new therapeutic approach to counter the age-related vascular complications has been a challenge. Hence, we evaluated the therapeutic effects of fenofibrate (FEN) in ageing. We also studied the FEN anti-ageing effects, and the possible molecular mechanisms that may clarify these effects. Methods: Thirty male rats were equally divided into three groups: 1Control group: Received (1% CMC) via gastric lavage, 2aged non treated group: Received (1% CMC) via gastric lavage, and 3Aged (FEN) treated group: received FEN (30 mg/kg) dissolved in (1% CMC) via gastric lavage for 7 weeks. At the end of study, serum lipid profile, inflammatory and oxidative stress markers were performed. Lastly, rats were subjected for measurement of invasive mean arterial blood pressure (MABP) and vascular reactivity to NE and Ach. Results: Aging resulted in dyslipidemia and elevation of inflammatory biomarkers, with altered oxidative stress markers. Increased MABP and decreased vascular reactivity to NE and Ach were also notice. FEN restored significantly aged related biochemical and vascular pathophysiological changes. Conclusion: FEN could be a good candidate for cardiovascular deficits due to ageing via amelioration of dyslipidemia, inflammation, attenuation of oxidative stress, reduction of MABP and improvement of vascular reactivity.","PeriodicalId":9347,"journal":{"name":"Bulletin of Egyptian Society for Physiological Sciences","volume":"11 1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86185705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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