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Washing effect of minced fish muscle in water on degradation of polyphosphates during manufacturing process of surimi-based products 鱼糜在水中的洗涤对鱼糜制品生产过程中多磷酸盐降解的影响
Pub Date : 1992-01-01 DOI: 10.2331/SUISAN.58.79
A. Matsunaga, T. Ooizumi, A. Yamamoto, K. Kawasaki, E. Mizukami
The washing effect of minced fish muscle on the degradation of polyphosphates (PP) in the salted meat paste (SMP) during the setting process was studied. Surimi MWO-MW4 were made from minced Walleye pollack muscle by washing 0-4 times with a buffer solution (pH 7.5). Aliquots of the SMP MWO-MW4, which were mixtures of surimi MWO-MW4, NaCl, and tripolyphosphate (P3), were incubated at 20°C. Degradations of P3 and pyrophosphate (P2) in the SMP during the setting process were determined. In the SMP MWO, P3 was rapidly degraded to P2 and P2 thus formed was also rapidly degraded to orthophosphate. As the number of washing times increased, the degradation rates of both P3 and P2 slowed down and P2 became hard to be degraded in SMP MW2-MW4. In order to determine the mechanism of this phenomenon, the amounts of tripolyphosphatase (P3ase), pyrophosphatase (P2ase) and magnesium (Mg) as an enzyme activator in surimi MWO-MW4 were measured. Along with the washings, P2ase and Mg were washed out from minced muscle, but P3ase was not. The decrease in Mg concentration suppressed P3ase activity in the SMP. Thus, repeated washing of minced muscle seemed to have an effect to degrade P3 and P2 in the SMP during the setting process.
研究了鱼糜洗涤对咸肉膏(SMP)凝固过程中多磷酸盐(PP)降解的影响。鱼糜MWO-MW4是用明太鱼肌肉绞碎,用pH为7.5的缓冲液洗涤0-4次制成。将鱼糜MWO-MW4、NaCl和三聚磷酸盐(P3)的混合物等分的SMP MWO-MW4在20℃下孵育。测定了定型过程中SMP中P3和焦磷酸盐(P2)的降解情况。在SMP MWO中,P3被快速降解为P2,由此形成的P2也被快速降解为正磷酸盐。随着洗涤次数的增加,P3和P2的降解速度减慢,P2在SMP MW2-MW4中变得难以降解。为了确定这一现象的机理,测定了鱼糜MWO-MW4中三聚磷酸酶(P3ase)、焦磷酸酶(P2ase)和酶激活剂镁(Mg)的含量。随着洗涤,P2ase和Mg从绞碎的肌肉中被洗去,但P3ase没有。Mg浓度的降低抑制了SMP中P3ase的活性。因此,在凝固过程中,反复洗涤切碎的肌肉似乎对SMP中的P3和P2有降解作用。
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引用次数: 5
Factors influencing texturization of sarcoplasmic protein of fish by high pressure treatment 高压处理对鱼类肌浆蛋白构化的影响因素
Pub Date : 1992-01-01 DOI: 10.2331/SUISAN.58.2197
E. Okazaki, Koji Nakamura
For the purpose of the utilization of sarcoplasmic protein of fish (Sp-P) as a food material, the behavior of the Sp-P under a high pressure was examined. Sp-P from sardine, walleye pollack, flatfish, and horse mackerel was observed to be insolubilized and precipitated at a pressure above 1, 500kg/cm2. When the concentration of Sp-P was above 50mg/ml, the protein was texturized. The characteristics of the texturized product were affected by many factors such as fish species, pH, protein concentration, pressure, and treatment time. An observation under a scanning electron microscope and physical measurement revealed that the structure of the texturized material was porous but elastic, and it was quite different from that of the coagulum obtained from Sp-P by heat treatment. It is concluded that pressurization might be an advantageous treatment for the texturization of Sp-P.
为了将鱼肌浆蛋白(Sp-P)作为一种食品原料加以利用,研究了Sp-P在高压条件下的行为。从沙丁鱼、白目鱼、比目鱼和马鲛鱼中提取的Sp-P在超过1,500 kg/cm2的压力下被观察到不溶解和沉淀。当Sp-P浓度大于50mg/ml时,蛋白呈织构化。鱼的种类、pH值、蛋白质浓度、压力、处理时间等因素对制品的特性都有影响。扫描电镜观察和物理测量结果表明,织构材料的结构具有多孔性和弹性,与Sp-P热处理后的凝固体有很大的不同。结果表明,加压可能是有利于Sp-P织构化的处理方法。
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引用次数: 10
Growth, survival, and glycogen content of triploid Pacific oyster Crassostrea gigas in the water of Hiroshima, Japan 日本广岛水域三倍体太平洋牡蛎长牡蛎的生长、存活和糖原含量
Pub Date : 1992-01-01 DOI: 10.2331/SUISAN.58.1063
S. Akashige, Tooru Fushimi
Triploidy was induced in the Pacific oyster Crassostrea gigas by treating newly fertifiized eggs with cytochalasin B in July 1987 and 1988. Growth, survival, glycogen content, and moisture content of triploid oysters cultured in the waters of Hiroshima were investigated. In the spawning season (July to September) shell height, total weight and soft body weight of diploids decreased or failed to increase, while those of triploids continued to increase. At the end of September the mean values of shell height, total weight and soft body weight of triploids were 1.0 to 1.2, 1.4 to 1.9, and 1.8 to 2.5 times respectively as much as those of diploids. The difference of soft body weight between diploids and triploids suggests that diploids lost 44 to 60% of wet soft body weight or 56 to 67% of dry soft body weight as gametes spawned. Condition index, which means the ratio of soft body weight against total body weight including shells, decreased sharply in diploids in the spawning season owing to mass spawning, while this sharp decrease was not observed in triploids. In diploids, the moisture content of the soft body, which was around 80% before the spawning season, increased 83 to 84% in the spawning season. But in the case of triploids, moisture content was at a low level of 77 to 82%. The glycogen content of diploids decreased to a low level of 2.7 to 5.4% on a dry basis according to gametogenesis, while that of triploids was at a high level of 8.5 to 16.9%. These results are due to the retardation of gonado- and gametogenesis. Mortality of triploids was about half of diploids from September to November, which suggests that the decrease in physiological activities must be smaller in triploids than in diploids because of the retardation of gonado- and gametogenesis.
1987年7月和1988年7月,用细胞松弛素B处理新受精卵诱导太平洋牡蛎长牡蛎三倍体。研究了在广岛海域养殖的三倍体牡蛎的生长、存活、糖原含量和水分含量。产卵季节(7 ~ 9月),二倍体的壳高、总重和软体重下降或不增加,而三倍体的壳高、总重和软体重继续增加。9月末,三倍体的壳高、总重和软体重的平均值分别是二倍体的1.0 ~ 1.2倍、1.4 ~ 1.9倍和1.8 ~ 2.5倍。二倍体和三倍体的软体质量差异表明,在配子产生后,二倍体湿软体质量损失44% ~ 60%,干软体质量损失56% ~ 67%。二倍体在产卵季节由于大量产卵,软体重与包括壳在内的总重之比的条件指数急剧下降,而三倍体则没有出现这种急剧下降。二倍体产卵季节前软体含水量约为80%,产卵季节后软体含水量增加83 ~ 84%。而在三倍体中,水分含量较低,为77% ~ 82%。根据配子体发育,二倍体的糖原含量在干基础上下降到2.7 ~ 5.4%的低水平,而三倍体的糖原含量在8.5 ~ 16.9%的高水平。这些结果是由于性腺和配子发生的迟缓。9 ~ 11月三倍体的死亡率约为二倍体的一半,说明由于性腺和配子体发育迟缓,三倍体的生理活性下降幅度小于二倍体。
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引用次数: 43
Comparison of lipid properties between wild and cultured ayu 野生与养殖阿育鱼脂质特性比较
Pub Date : 1991-01-01 DOI: 10.2331/SUISAN.57.1965
H. Nakagawa, Y. Takahara, G. R. Nematipour
Lipid properties were compared between cultured and wild ayu Plecoglossus altivelis obtained from 9 different producing districts of Japan. The cultured ayu was characterized by high accumulation of triglycerides in intraperitoneal fat body (IPF) and muscle. While the IPF ratio of wild ayu ranged between 0.1 and 8.6% (average 1.8•}1.6%), that of cultured ayu ranged between 1.5 and 15.1% (average 7.5•}3.6%). The muscle lipid level of cultured ayu (8.2•}2.5%) was extremely higher than that of wild ayu (3.4•}1.7%). However, the ranges of both IPF ratio and muscle lipid level of cultured ayu overlapped with those of the wild ayu. Fatty acid composition of muscle triglycerides was compared between cultured and wild ayu. According to muscle lipid level and fatty acid composition, the ayu could be divided into two groups. One group consisted mainly of the samples obtained from cultured ayu, and the other group from wild ayu. However, there were samples in the cultured ayu which could be categorized to the wild fish group. The results show the possibility that technical advancement will enable us to reduce the IPF ratio and muscle lipid level of cultured ayu to•@the values of wild ayu.
比较了日本9个不同产地的养殖和野生阿育的脂质特性。培养的阿育鱼具有甘油三酯在腹腔脂肪体(IPF)和肌肉中大量积累的特点。野生ayu的IPF值为0.1 ~ 8.6%(平均1.8•}1.6%),养殖ayu的IPF值为1.5 ~ 15.1%(平均7.5•}3.6%)。养殖阿鱼的肌脂水平(8.2•}2.5%)极显著高于野生阿鱼(3.4•}1.7%)。然而,养殖阿鱼的IPF比率和肌脂水平的变化范围与野生阿鱼有重叠。比较了养殖和野生阿育鱼肌肉甘油三酯的脂肪酸组成。根据肌肉脂质水平和脂肪酸组成可分为两组。一组以养殖阿玉为主要样本,另一组以野生阿玉为主要样本。然而,在养殖的阿育鱼中有样本可归类为野生鱼类。结果表明,技术的进步使我们有可能将培养的鱼的IPF比和肌脂水平降低到野生鱼的1 / 2。
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引用次数: 11
Changes in ATP related compounds and IMP-degrading enzyme activity of New Zealand golden snapper [Pagrus auratus] 新西兰金鲷[Pagrus auratus] ATP相关化合物及imp降解酶活性的变化
Pub Date : 1991-01-01 DOI: 10.2331/SUISAN.57.329
K. Nedachi, N. Hirota
The amounts of ATP related compounds during storage and some properties of IMP degrading enzyme in the ordinary muscle of the golden snapper Pagrus auratus from waters around New Zealand were examined. When the muscle were stored for five days at 4°C, IMP remarkably decreased and hypoxanthine increased, whereas the amount of inosine was low and scarcely changed during storage. The amounts of IMP in the muscle hardly changed during storage for 43days at-20°C. IMP was degraded optimally at pH6.2 and pH8.4 in dorsal muscle homogenate of golden snapper. The IMP-degrading activity in the minced dorsal muscle decreased by the storage for one day at 4°C, showing 82.5% of initial activity, and since then, the activity decreased rapidly, showing 17.5% after seven days of storage. The IMP-degrading activity of crude enzyme extracted from the golden snapper muscle using the detergent remarkably increased at pH8.0 in the presence of 2mM Mg2+ and stabilized at pH7.0 for 48h (2±1°C).
研究了新西兰海域金鲷鱼(Pagrus auratus)普通肌肉中ATP相关化合物的含量和IMP降解酶的一些特性。在4℃下保存5 d, IMP显著降低,次黄嘌呤显著增加,而肌苷含量较低,在保存过程中几乎没有变化。在20°C下保存43天,肌肉中IMP的含量几乎没有变化。在pH6.2和pH8.4条件下,IMP在金鲷鱼背肌匀浆中降解效果最佳。4℃条件下保存1天后,背肌中imp降解活性下降,为初始活性的82.5%,此后活性迅速下降,保存7天后为17.5%。在pH8.0和2mM Mg2+存在下,该洗涤剂对金鲷鱼肌肉粗酶的imp降解活性显著提高,并在pH7.0稳定48h(2±1℃)。
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引用次数: 9
Changes in serum steroid hormone levels and in vitro steroid hormone production in the ovaries of female chum salmon Oncorhynchus keta during spawning migration. 雌鲑产卵洄游过程中血清类固醇激素水平及卵巢体外类固醇激素分泌的变化。
Pub Date : 1991-01-01 DOI: 10.2331/SUISAN.57.1111
H. Ueda, O. Hiroi, K. Yamauchi, A. Hara, H. Kagawa, S. Adachi, Y. Nagahama
Following the previous study, serum steroid hormone profiles and in vitro steroid hormone production in the testes of male chum salmon Oncorhynchus keta were examined by adding materials sampled at the North Pacific Ocean. The maximum serum level of 11-ketotestos-terone (11-keto T; 56ng/ml at the coastal sea) preceded that of testosterone (T; 48ng/ml at the pre-spawning period). Levels of both androgens in serum decreased in the spawning period when serum 17α, 20β-dihydroxy-4-pregnen-3-one (17α, 20β-diOHprog) level rapidly in-creased. Incubation of testicular fragments from testes of the coastal sea and the pre-spawning period with 17α-hydroxyprogesterone (17α-OHprog; 0.1μg/ml), T (0.1μg/ml) and chum salmon gonadotropin (SGA; 1μg/ml) resulted in elevated 11-keto T concentration in the incubation medium. 17α, 20β-DiOHprog level in medium was highly increased only in the presence of 17α-OHprog. The conversion of 17α-OHprog to 17α, 20β-diOHprog was enhanced by adding SGA to the incubation medium of testicular fragments of individuals collected from the river (pre-spawning period), but not from the coastal sea. These results indicate that 11-lceto T and 17α, 20β-diOHprog respectively are the major steroids responsible for latter phase of sperma-togenesis and spermiation in male chum salmon.
在前人研究的基础上,通过添加北太平洋取样的材料,研究了雄性鲑鱼Oncorhynchus keta的血清类固醇激素谱和睾丸中体外类固醇激素的产生。11-酮睾酮(11-酮T;56ng/ml)高于睾酮(T;产卵前48ng/ml)。产卵期血清17α, 20β-二羟基-4-孕激素-3-one (17α, 20β-diOHprog)水平迅速升高,两种雄激素水平均下降。17α-羟孕酮(17α-OHprog;0.1μg/ml)、T (0.1μg/ml)和鲑鱼促性腺激素(SGA);1μg/ml)导致培养液中11-酮T浓度升高。培养基中的17α, 20β-DiOHprog水平仅在17α- ohprog存在时才显著升高。在河流(产卵前)收集的个体睾丸碎片孵育液中添加SGA可促进17α- ohprog向17α, 20β-diOHprog的转化,而在沿海海域收集的个体睾丸碎片孵育液中不添加SGA。这些结果表明,11-乙酰胆碱酮和17α, 20β-二羟孕酮分别是雄性大马哈鱼精子发生和精子形成后期的主要激素。
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引用次数: 10
Feeding-deterrent effect of phlorotannins from the brown alga Ecklonia stolonifera against the abalone Haliotis discus hannai 褐藻绿单宁对鲍鱼的阻食作用
Pub Date : 1991-01-01 DOI: 10.2331/SUISAN.57.2065
K. Taniguchi, K. Kurata, Minoru Suzuki
For the purpose of clarifying the reason why the laminariaceous brown alga Ecklonia stolonifera are not grazed by marine herbivorous animals, such as abalones and sea urchins, feeding-deterrent effect of the methanol extract from this alga was tested on the abalone Haliotis discus hannai by the bioassay with cellulose plate method. Concurrently, another two laminariaceous algae Eisenia bicyclis and Ecklonipopsis radicosa were examined. A large amount of the water-soluble fraction was obtained from the methanol extract of Eck. stolonifera showed potent feeding-deterrent activity when compared with those from the other two species. The active compounds that were isolated from the water-soluble fraction by a combination of column (Sephadex LH-20) and thin-layer (silica gel) chromatography, were characterized as phloroglucinol oligomers and polymers, phlorotannins, on the basis of spectroscopic analysis. The feeding-deterrent activity of phlorotannins depends upon the degree of polymerization of phloroglucinol (the polarity of the polyphenol). We concluded that the algal phlorotannins play an important role in the chemical defense against marine herbivores.
为了阐明浅层褐藻不被鲍鱼、海胆等海洋草食性动物食用的原因,采用纤维素平板法测定了浅层褐藻甲醇提取物对鲍鱼褐藻的阻食作用。同时,对另外两种层状藻进行了研究,分别是双环爱森藻和根状Ecklonipopsis。从Eck的甲醇提取物中获得了大量的水溶性组分。与其他两种相比,匍匐茎具有较强的取食威慑活性。通过色谱柱(Sephadex LH-20)和薄层(硅胶)色谱相结合的方法从水溶性组分中分离得到活性化合物,经波谱分析鉴定为间苯三酚低聚物和间苯三酚聚合物。间苯三酚的阻食活性取决于间苯三酚聚合的程度(多酚的极性)。我们认为藻绿单宁在海洋食草动物的化学防御中起着重要的作用。
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引用次数: 27
Effect of polar and nonpolar lipids from krill on quality of eggs of red seabream Pagrus major 磷虾极性脂质和非极性脂质对红鲷卵品质的影响
Pub Date : 1991-01-01 DOI: 10.2331/suisan.57.695
Takeshi Watanabe, T. Fujimura, Min-Jer Lee, K. Fukusho, S. Satoh, T. Takeuchi
Red seabream broodstock were fed on various diets shortly before spawning to reexamine the effectiveness of phospholipid and astaxanthins for the improvement of egg quality in comparison to frozen raw krill or vitamin E. The rate of buoyant eggs was lowest in the broodstock fed the control diet, and the value was elevated by supplementation of 200mg α-tocopherol, 2.5% krill polar lipids and 2.5% krill non-polar lipids or by feeding frozen raw krill. The rate of hatching was also improved by inclusion of one of these substances into the control diet. These results suggested that the effective components in raw krill for improving the quality of red seabream eggs are both phosphatidyl choline of the polar lipid fraction and astaxanthins of the polar lipid fraction. Therefore, the principal factors aiding the reproduction of red seabream are the common factors between these substances, probably free radical scavengers.
在产卵前饲喂不同饲料,对比冷冻生磷虾和维生素e,重新检验磷脂和虾青素对改善蛋品质的效果。饲喂对照饲料的红鲷的浮力蛋率最低,通过添加200mg α-生育酚、2.5%磷虾极性脂和2.5%磷虾非极性脂或饲喂冷冻生磷虾可提高浮力蛋率。在对照日粮中加入其中一种物质也能提高孵化率。综上所述,生磷虾中提高红鲷蛋品质的有效成分是极性脂质部分的磷脂酰胆碱和极性脂质部分的虾青素。因此,帮助红鲷繁殖的主要因素是这些物质之间的共同因素,可能是自由基清除剂。
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引用次数: 54
Effects of coexisting Ulva pertusa on the production of kuruma prawn [Penaeus japonicus] 共生褐藻对日本对虾产量的影响
Pub Date : 1991-01-01 DOI: 10.2331/SUISAN.57.1597
E. Danakusumah, S. Kadowaki, H. Hirata
(DGR) of the prawns was calculated using the formula of Deshimaru and Shigeno.5) DGR of U. pertusa was also calculated using the same formula, but expressed as a weekly average. Results showed that prawns cultured with U. pertusa have better production, survival rate, and feed coefficient than those cultured without the alga (Table 1). The production of prawns cultured with U. pertusa in Exp-1 and Exp-2 were 69% and 54% higher respectively than those cultured without U. pertusa. The survival rate of prawns cultured with U. pertusa in Exp-1 and Exp-2 were 67% and 21% higher respectively than those cultured without U. pertusa. The feed coefficient of prawns cultured with U. pertusa in Exp-1 and Exp-2 were 61% and 43% higher respectively than those cultured without U. pertusa. The plant-animal production ratio was found to be nearly the same in both experiments. The present study suggests that the coexistence of U. pertusa in the prawn culture system increased the production and survival of the prawn. The presence of U. pertusa is able to support a better condition of the culture system.
(DGR)采用Deshimaru和shigenu的公式计算。5)pertusa的DGR也采用相同的公式计算,但以周平均值表示。结果表明,添加褐藻的对虾产量、成活率和饲料系数均优于不添加褐藻的对虾(表1)。Exp-1和Exp-2添加褐藻的对虾产量分别比不添加褐藻的提高69%和54%。在Exp-1和Exp-2中添加紫苏菌的对虾的存活率分别比不添加紫苏菌的对虾高67%和21%。在Exp-1和Exp-2中添加紫苏菌的对虾的饲料系数分别比不添加紫苏菌的对虾高61%和43%。在两个实验中,发现植物与动物的生产比几乎相同。本研究表明,在对虾养殖系统中,佩尔图沙菌的共存提高了对虾的产量和存活率。紫苏菌的存在能够为培养系统提供更好的条件。
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引用次数: 13
Triploidization of Haliotis diversicolor diversicolor by cold shock 冷激三倍体分化的研究
Pub Date : 1991-01-01 DOI: 10.2331/SUISAN.57.1263
M. Kudo, K. Arai, K. Fujino
{"title":"Triploidization of Haliotis diversicolor diversicolor by cold shock","authors":"M. Kudo, K. Arai, K. Fujino","doi":"10.2331/SUISAN.57.1263","DOIUrl":"https://doi.org/10.2331/SUISAN.57.1263","url":null,"abstract":"","PeriodicalId":9361,"journal":{"name":"Bulletin of the Japanese Society of Scientific Fisheries","volume":"78 21","pages":"1263-1267"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91407206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
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Bulletin of the Japanese Society of Scientific Fisheries
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