Vesicouterine fistula is a rare but increasing phenomenon in obstetric practice. Early diagnosis and treatment will go a long way to reduce the associated morbidity as well as improve the quality of life of its victims. Our case exemplifies how the use of simple techniques can diagnose and bring great relief to such patients.
{"title":"Early Diagnosis and Treatment of Vesicouterine Fistula Using Low-Tech Methods in Low Resource Setting of Northern Ghana. A Case Report","authors":"","doi":"10.33140/jgrm.05.02.15","DOIUrl":"https://doi.org/10.33140/jgrm.05.02.15","url":null,"abstract":"Vesicouterine fistula is a rare but increasing phenomenon in obstetric practice. Early diagnosis and treatment will go a long way to reduce the associated morbidity as well as improve the quality of life of its victims. Our case exemplifies how the use of simple techniques can diagnose and bring great relief to such patients.","PeriodicalId":93778,"journal":{"name":"Journal of gynecology, clinical obstetrics and reproductive medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73469421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The incidence of early onset neonatal GBS(EOGBS) disease in the UK and Ireland is 0.57/1000 births. Intrapartum antibiotic prophylaxis (IAP) reduces the risk. Previous colonisation is associated with 50% carriage in the current pregnancy. In these women, RCOG recommends IAP with a history of neonatal infection, otherwise offering the option of screening at 35-37 weeks. In Ireland, there is no national consensus on IAP in prior GBS colonisation. Currently at University Hospital Waterford (UHW), all women with prior GBS colonisation receive IAP. Studies examining the use of point-of-care testing have shown reduction in the use of IAP and EOGBS rates. We aimed to examine the screening and IAP administration in maternal prior GBS colonisation and the incidence of GBS in this cohort in UHW. Data was collected retrospectively from laboratory, medical records and electronic patient manager systems. Women who received IAP between 1stJuly 2020 and 31stDecember 2020 were identified. Women who received IAP for current and prior GBS colonisation were included. Women who received IAP for preterm labour, preterm prelabour rupture of membranes and pyrexia in labour were excluded. Ninety-two women with current or prior GBS colonisation received IAP, of which only 15(16.30%) were current and 77(83.69%) were prior GBS colonisation. In women with prior GBS colonisation, 49(63.63%) were screened, 3/49(6.12%) were positive, 28 were not screened. Seventy-eight (84.78%) received benzyl-penicillin. Six (6.52%) received clindamycin. Twenty-two (23.91%) babies were admitted to the Neonatal Unit, however, only one cultured positive for gram-positive cocci. The incidence of EOGBS in this cohort is low. A risk-based approach or point-of-care testing should be considered to reduce unnecessary IAP administration.
{"title":"Need for Intrapartum Antibiotic Prophylaxis in Women with Prior History of Group B Streptococcus Carriage","authors":"","doi":"10.33140/jgrm.05.02.14","DOIUrl":"https://doi.org/10.33140/jgrm.05.02.14","url":null,"abstract":"The incidence of early onset neonatal GBS(EOGBS) disease in the UK and Ireland is 0.57/1000 births. Intrapartum antibiotic prophylaxis (IAP) reduces the risk. Previous colonisation is associated with 50% carriage in the current pregnancy. In these women, RCOG recommends IAP with a history of neonatal infection, otherwise offering the option of screening at 35-37 weeks. In Ireland, there is no national consensus on IAP in prior GBS colonisation. Currently at University Hospital Waterford (UHW), all women with prior GBS colonisation receive IAP. Studies examining the use of point-of-care testing have shown reduction in the use of IAP and EOGBS rates. We aimed to examine the screening and IAP administration in maternal prior GBS colonisation and the incidence of GBS in this cohort in UHW. Data was collected retrospectively from laboratory, medical records and electronic patient manager systems. Women who received IAP between 1stJuly 2020 and 31stDecember 2020 were identified. Women who received IAP for current and prior GBS colonisation were included. Women who received IAP for preterm labour, preterm prelabour rupture of membranes and pyrexia in labour were excluded. Ninety-two women with current or prior GBS colonisation received IAP, of which only 15(16.30%) were current and 77(83.69%) were prior GBS colonisation. In women with prior GBS colonisation, 49(63.63%) were screened, 3/49(6.12%) were positive, 28 were not screened. Seventy-eight (84.78%) received benzyl-penicillin. Six (6.52%) received clindamycin. Twenty-two (23.91%) babies were admitted to the Neonatal Unit, however, only one cultured positive for gram-positive cocci. The incidence of EOGBS in this cohort is low. A risk-based approach or point-of-care testing should be considered to reduce unnecessary IAP administration.","PeriodicalId":93778,"journal":{"name":"Journal of gynecology, clinical obstetrics and reproductive medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78126626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: This facility-based retrospective cross sectional design study examined the prevalence, risk factors and management of hypertensive disorders of pregnancy among pregnant women attending antenatal clinic in the University of Benin Teaching Hospital, Benin City Materials and Methods: A stratified proportionate sampling technique were adopted to review a total of 358 secondary data of patients who were managed for hypertensive disorders of pregnancy from June 2015 – June 2020 in the study institution. Data were analyzed using descriptive statistics, multinomial regression analysis at 0.05 the level of significance for all measured variables. Results: The mean ± SD = 31.15 ± 5.954 years. More than half 60.3% of them were multigravida. 60.1% were in second trimester. Mean BP at booking was 146/95 mmHg. 56.1% were overweight while 17.6% were obese. The mean ± SD ANC visits was 10.71 ± 5.411 times. The five-year prevalence rate of hypertensive disorders of pregnancy among pregnant women in the study setting is 10.9%. Conclusion: There is high prevalence of HDP in the study institution with preeclampsia most prevalent. Therefore, we strongly recommend the utilization of screening tools for the prediction, early diagnoses as well as timely intervention HDP
{"title":"Prevalence, Risk Factors and Management of Hypertensive Disorders of Pregnancy Among Pregnant Women in University of Benin Teaching Hospital, Benin City, Nigeria","authors":"","doi":"10.33140/jgrm.05.02.12","DOIUrl":"https://doi.org/10.33140/jgrm.05.02.12","url":null,"abstract":"Background: This facility-based retrospective cross sectional design study examined the prevalence, risk factors and management of hypertensive disorders of pregnancy among pregnant women attending antenatal clinic in the University of Benin Teaching Hospital, Benin City Materials and Methods: A stratified proportionate sampling technique were adopted to review a total of 358 secondary data of patients who were managed for hypertensive disorders of pregnancy from June 2015 – June 2020 in the study institution. Data were analyzed using descriptive statistics, multinomial regression analysis at 0.05 the level of significance for all measured variables. Results: The mean ± SD = 31.15 ± 5.954 years. More than half 60.3% of them were multigravida. 60.1% were in second trimester. Mean BP at booking was 146/95 mmHg. 56.1% were overweight while 17.6% were obese. The mean ± SD ANC visits was 10.71 ± 5.411 times. The five-year prevalence rate of hypertensive disorders of pregnancy among pregnant women in the study setting is 10.9%. Conclusion: There is high prevalence of HDP in the study institution with preeclampsia most prevalent. Therefore, we strongly recommend the utilization of screening tools for the prediction, early diagnoses as well as timely intervention HDP","PeriodicalId":93778,"journal":{"name":"Journal of gynecology, clinical obstetrics and reproductive medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80333347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study includes 20 cases of PPH seen during Caesarean section by a single surgeon Dr. Sadhna Mathur during the last 11 years where this hemostatic suture was used to control PPH from placental site in uterine cavity, after the medical measures failed to control the bleeding from placental site (uterine cavity). The bleeding point was localized and a haemostatic suture in the overlying uterine musculature was applied. It was found to be completely effective hence it is being shared through this paper
{"title":"Management of PPH (Placental Site Bleeding) by a Direct Haemostatic Suture in True PPH during Caesarean section: A New Approach","authors":"","doi":"10.33140/jgrm.05.02.04","DOIUrl":"https://doi.org/10.33140/jgrm.05.02.04","url":null,"abstract":"This study includes 20 cases of PPH seen during Caesarean section by a single surgeon Dr. Sadhna Mathur during the last 11 years where this hemostatic suture was used to control PPH from placental site in uterine cavity, after the medical measures failed to control the bleeding from placental site (uterine cavity). The bleeding point was localized and a haemostatic suture in the overlying uterine musculature was applied. It was found to be completely effective hence it is being shared through this paper","PeriodicalId":93778,"journal":{"name":"Journal of gynecology, clinical obstetrics and reproductive medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89095844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Respectful maternity care (RMC) is an approach to care based on personal choice and preferences, which supports and promotes, and doesn’t undermine a person’s self-respect [1]. During the past three decades, maternal mortality has decreased nearly by half but is still alarmingly high, particularly in sub-Saharan African countries. More than half of maternal mortality is associated with poor quality of care, and most maternal mortality is preventable if timely quality maternity care is delivered and evidence-based action is taken [2-4]. Responding to this, the 2030 Sustainable Development Goals include reducing the maternal mortality rate below 77 per 100,000 live births and eliminating all forms of violence against women, particularly during labor and delivery [5]. The mistreatment of women during institutional delivery could be an obstacle to these ambitious goals, especially in a low-income country like Ethiopia[6].
{"title":"Barriers to Respectful Maternity Care from Mother’s, Provider’s and Administrator’s Perspective: An Exploratory Qualitative Study","authors":"","doi":"10.33140/jgrm.05.02.07","DOIUrl":"https://doi.org/10.33140/jgrm.05.02.07","url":null,"abstract":"Respectful maternity care (RMC) is an approach to care based on personal choice and preferences, which supports and promotes, and doesn’t undermine a person’s self-respect [1]. During the past three decades, maternal mortality has decreased nearly by half but is still alarmingly high, particularly in sub-Saharan African countries. More than half of maternal mortality is associated with poor quality of care, and most maternal mortality is preventable if timely quality maternity care is delivered and evidence-based action is taken [2-4]. Responding to this, the 2030 Sustainable Development Goals include reducing the maternal mortality rate below 77 per 100,000 live births and eliminating all forms of violence against women, particularly during labor and delivery [5]. The mistreatment of women during institutional delivery could be an obstacle to these ambitious goals, especially in a low-income country like Ethiopia[6].","PeriodicalId":93778,"journal":{"name":"Journal of gynecology, clinical obstetrics and reproductive medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81942787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Initiation of family planning use during the postpartum period is crucial for both maternal and child health. Moreover, this period is the extension of maternal health care and its level of uptake is built up on the level of continuum of care of maternal health care utilization. Continuum of maternal health care including, antenatal care, skilled delivery and postnatal care is crucial for women’s reproductive health. Despite that, there was no data regarding with the importance of continuum of care of maternal health care on the postpartum modern contraceptive utilization. Thus, the aim of this study was to assess the association of the continuum of maternal care on subsequent timely use of postpartum family planning. Methods: a community based cross sectional study was employed on 828 women. A multistage sampling technique was used to enroll the study participants. The data were collected by a semi-structured and pretested, face-toface, interviewer administered questionnaire. Data were entered into EpiData 3.1 and analyzed by using SPSS 23 software. Bivariate and multivariable logistic regression analyses were used to declare the association between dependent and independent variables. Odds ratio, its 95% confidence interval (CI) and P-value <0.05 were used to determine the significance level. Results: the prevalence of timely initiation of postpartum contraceptive methods was 16.1%. Having of postpartum care (AOR: 2.0(95%CI: 1.2, 3.2)), completion of delivery level continuum of care (AOR: 2.9(95%CI: 1.4, 6.1)), and completion of postnatal level continuum of care (AOR: 4.0(95%CI: 2.0, 7.8)) were factors of timely intuition of postpartum contraceptive methods. Conclusion and recommendation: The prevalence of timely initiation of postpartum contraceptive methods was low. The finding of this study implies that the need of strengthening the connection between maternal continuum of care and reproductive components of continuum of care.
{"title":"Maternal Continuum of Care as Independent Determinants for Timely Initiation of Modern Contraceptive Methods Utilization Among Postnatal Women in Arba Minch Zuria District, Southern Ethiopia","authors":"D. Haile","doi":"10.33140/jgrm.05.02.02","DOIUrl":"https://doi.org/10.33140/jgrm.05.02.02","url":null,"abstract":"Background: Initiation of family planning use during the postpartum period is crucial for both maternal and child health. Moreover, this period is the extension of maternal health care and its level of uptake is built up on the level of continuum of care of maternal health care utilization. Continuum of maternal health care including, antenatal care, skilled delivery and postnatal care is crucial for women’s reproductive health. Despite that, there was no data regarding with the importance of continuum of care of maternal health care on the postpartum modern contraceptive utilization. Thus, the aim of this study was to assess the association of the continuum of maternal care on subsequent timely use of postpartum family planning. Methods: a community based cross sectional study was employed on 828 women. A multistage sampling technique was used to enroll the study participants. The data were collected by a semi-structured and pretested, face-toface, interviewer administered questionnaire. Data were entered into EpiData 3.1 and analyzed by using SPSS 23 software. Bivariate and multivariable logistic regression analyses were used to declare the association between dependent and independent variables. Odds ratio, its 95% confidence interval (CI) and P-value <0.05 were used to determine the significance level. Results: the prevalence of timely initiation of postpartum contraceptive methods was 16.1%. Having of postpartum care (AOR: 2.0(95%CI: 1.2, 3.2)), completion of delivery level continuum of care (AOR: 2.9(95%CI: 1.4, 6.1)), and completion of postnatal level continuum of care (AOR: 4.0(95%CI: 2.0, 7.8)) were factors of timely intuition of postpartum contraceptive methods. Conclusion and recommendation: The prevalence of timely initiation of postpartum contraceptive methods was low. The finding of this study implies that the need of strengthening the connection between maternal continuum of care and reproductive components of continuum of care.","PeriodicalId":93778,"journal":{"name":"Journal of gynecology, clinical obstetrics and reproductive medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80702139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The pseudo-unicornuate uterus which is a rare uterine malformation, that can be the site of a pregnancy. Pregnancies in the rudimentary horn usually result in a rupture due to a uterine wall not adapted to the level of the horn, but especially diagnostic difficulties. Their discovery is most often made in intraoperative, when an indication of laparotomy is made for hemoperitoneum in emergency. They are therefore responsible for high maternal-fetal mortality and morbidity. We report in this observation a case of pregnancy in a rudimentary unruptured horn, in a 28-year-old, 4th gesture 2nd parous, the discovery of which was made incidentally, during an indication of laparotomy posed for suspected abdominal pregnancy complicated by a surgical abdomen.
{"title":"Pregnancy on a Rudimentary Unbroken Horn of a Pseudo-Unicornuate Uterus: A Case Report at The University Hospital of Angre Abidjan","authors":"","doi":"10.33140/jgrm.05.02.03","DOIUrl":"https://doi.org/10.33140/jgrm.05.02.03","url":null,"abstract":"The pseudo-unicornuate uterus which is a rare uterine malformation, that can be the site of a pregnancy. Pregnancies in the rudimentary horn usually result in a rupture due to a uterine wall not adapted to the level of the horn, but especially diagnostic difficulties. Their discovery is most often made in intraoperative, when an indication of laparotomy is made for hemoperitoneum in emergency. They are therefore responsible for high maternal-fetal mortality and morbidity. We report in this observation a case of pregnancy in a rudimentary unruptured horn, in a 28-year-old, 4th gesture 2nd parous, the discovery of which was made incidentally, during an indication of laparotomy posed for suspected abdominal pregnancy complicated by a surgical abdomen.","PeriodicalId":93778,"journal":{"name":"Journal of gynecology, clinical obstetrics and reproductive medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90542402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acute Fatty Liver of Pregnancy (AFLP), though rare, is an obstetric emergency which carries a high incidence of maternal and perinatal mortality, despite optimal care. It is a catastrophic disease affecting women in the third trimester of pregnancy or in the post-partum period. It is usually a diagnosis of exclusion and requires a strong index of suspicion for a timely diagnosis and prompt intervention. We report a case of 24-year-old prime at 34- week gestation, presented with malaise, nausea, vomiting, jaundice, disorientation and absent fetal movements. Intrauterine death of fetus occurred. Supportive management in an intensive care unit resulted in successful outcome. By this case report we once again wish to emphasize the value of a timely diagnosis with a high level of clinical suspicion and supportive laboratory investigations including imaging; the need for early termination of pregnancy; and adequate supportive care as the key management options for AFLP. Further, we wish to explore the current treatment options available for AFLP and discuss a few novel therapeutic strategies such as plasma exchange in treating such cases and the pros and cons associated with these treatment modalities.
{"title":"Acute Fatty Liver of Pregnancy Presenting as Fulminant Hepatic Failure: An Uncommon Disease","authors":"","doi":"10.33140/jgrm.05.02.06","DOIUrl":"https://doi.org/10.33140/jgrm.05.02.06","url":null,"abstract":"Acute Fatty Liver of Pregnancy (AFLP), though rare, is an obstetric emergency which carries a high incidence of maternal and perinatal mortality, despite optimal care. It is a catastrophic disease affecting women in the third trimester of pregnancy or in the post-partum period. It is usually a diagnosis of exclusion and requires a strong index of suspicion for a timely diagnosis and prompt intervention. We report a case of 24-year-old prime at 34- week gestation, presented with malaise, nausea, vomiting, jaundice, disorientation and absent fetal movements. Intrauterine death of fetus occurred. Supportive management in an intensive care unit resulted in successful outcome. By this case report we once again wish to emphasize the value of a timely diagnosis with a high level of clinical suspicion and supportive laboratory investigations including imaging; the need for early termination of pregnancy; and adequate supportive care as the key management options for AFLP. Further, we wish to explore the current treatment options available for AFLP and discuss a few novel therapeutic strategies such as plasma exchange in treating such cases and the pros and cons associated with these treatment modalities.","PeriodicalId":93778,"journal":{"name":"Journal of gynecology, clinical obstetrics and reproductive medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72987577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ectopic pregnancy is a condition in which the fertilized ovum implants outside the uterus or attaches to an abnormal portion of the uterus, like cervix, cornea, myometrium. Such state occurs in 2% of all reported pregnancies. There are plenty of risk factors for this state. Fallopian tube surgery in the past, pelvic inflammatory disease is one of them. Undiagnosed ectopic pregnancy may lead to serious complications, like rupturing of the fallopian tube and massive internal bleeding. We would like to present a case, in which the patient was not aware of being pregnant, until she got the histopathology results after laparoscopic surgery because of tumor in the rectouterine pouch.
{"title":"Ectopic Pregnancy - Is It Always a Threat to A Woman’s Life?","authors":"","doi":"10.33140/jgrm.05.02.05","DOIUrl":"https://doi.org/10.33140/jgrm.05.02.05","url":null,"abstract":"Ectopic pregnancy is a condition in which the fertilized ovum implants outside the uterus or attaches to an abnormal portion of the uterus, like cervix, cornea, myometrium. Such state occurs in 2% of all reported pregnancies. There are plenty of risk factors for this state. Fallopian tube surgery in the past, pelvic inflammatory disease is one of them. Undiagnosed ectopic pregnancy may lead to serious complications, like rupturing of the fallopian tube and massive internal bleeding. We would like to present a case, in which the patient was not aware of being pregnant, until she got the histopathology results after laparoscopic surgery because of tumor in the rectouterine pouch.","PeriodicalId":93778,"journal":{"name":"Journal of gynecology, clinical obstetrics and reproductive medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90516699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: To investigate the effect and mechanism of ginsenoside Rg3 on the proliferation and metastasis of cervical. Methods: Cervical cancer cells HeLa were treated with different concentrations (0, 0.12, 0.24, 0.48 mmol/L) of ginsenoside Rg3, and then the survival rate of HeLa cells was detected by CCK-8 method, and the migration and invasion of HeLa cells were assessed using Transwell test, and expression of E-cadherin, N-cadherin, vimentin, Toll receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88), phosphorylated nuclear transcription factor κB p65 (P-NF-κB p65) proteins were calculated by Western blot. Results: After ginsenoside Rg3 (0.12, 0.24, 0.48 mmol/L) treatment, the survival rate, migration number, invasion number, and N-cadherer number, and N-cadherin, Vimentin, TLR4, MyD88, p-NF-κB p65 protein expression of HeLa cells were significantly reduced (P<0.05) Ginsenoside protein expression was significantly increased (P<0.05), and showed a concentration-dependent relationship. Conclusion: Ginsenoside Rg3 could inhibit the proliferation and metastasis of cervical cancer cells in vitro, and its mechanism might be related to the inhibition of NF-κB signaling pathway.
{"title":"Ginsenoside Rg3 Inhibit The Proliferation And Metastasis of Cervical Cancer Cells in Vitro By Regulating NF-κB Signaling Pathway","authors":"","doi":"10.33140/jgrm.05.02.01","DOIUrl":"https://doi.org/10.33140/jgrm.05.02.01","url":null,"abstract":"Objective: To investigate the effect and mechanism of ginsenoside Rg3 on the proliferation and metastasis of cervical. Methods: Cervical cancer cells HeLa were treated with different concentrations (0, 0.12, 0.24, 0.48 mmol/L) of ginsenoside Rg3, and then the survival rate of HeLa cells was detected by CCK-8 method, and the migration and invasion of HeLa cells were assessed using Transwell test, and expression of E-cadherin, N-cadherin, vimentin, Toll receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88), phosphorylated nuclear transcription factor κB p65 (P-NF-κB p65) proteins were calculated by Western blot. Results: After ginsenoside Rg3 (0.12, 0.24, 0.48 mmol/L) treatment, the survival rate, migration number, invasion number, and N-cadherer number, and N-cadherin, Vimentin, TLR4, MyD88, p-NF-κB p65 protein expression of HeLa cells were significantly reduced (P<0.05) Ginsenoside protein expression was significantly increased (P<0.05), and showed a concentration-dependent relationship. Conclusion: Ginsenoside Rg3 could inhibit the proliferation and metastasis of cervical cancer cells in vitro, and its mechanism might be related to the inhibition of NF-κB signaling pathway.","PeriodicalId":93778,"journal":{"name":"Journal of gynecology, clinical obstetrics and reproductive medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86438502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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