Pub Date : 2023-03-30DOI: 10.31489/2023bmg1/195-205
A.A. Tursunova, S. Alimkulov, A. Myrzakhmetov, M.A. Kanay, Aizhan Zh. Dostaeva, Gulmira Isakan
The article presents basic information about the state of the river network in the Balkash-Alakol basin. They consist of a list of rivers and temporary watercourses from 5 km in mountainous areas and from 10 km in lowland areas, and also include information on their main morphological and morphometric characteristics, which were presented only in the 1970s, after the 1970s such extensive research has not been done. The purpose of the study is to identify rivers and temporary streams, as well as to obtain basic hydrographic information through field surveys and with the help of modern GIS technologies. To achieve the goals, complex field work was carried out using modern hydrological and geodetic equipment and interpretation of space images of Sentinel 1,2 satellites using ArcGIS tools and programs. Each river and temporary watercourse record has a unique feature identifier in the geographic information system. The database is intended for use in regional studies in the field of hydrology, ecology, nature management, and is also an information source for the implementation of measures to monitor and restore rivers, organize water use in transboundary river basins.
{"title":"Basic hydrographic information about the rivers and temporary watercourses of the Balkash-Alakol basin","authors":"A.A. Tursunova, S. Alimkulov, A. Myrzakhmetov, M.A. Kanay, Aizhan Zh. Dostaeva, Gulmira Isakan","doi":"10.31489/2023bmg1/195-205","DOIUrl":"https://doi.org/10.31489/2023bmg1/195-205","url":null,"abstract":"The article presents basic information about the state of the river network in the Balkash-Alakol basin. They consist of a list of rivers and temporary watercourses from 5 km in mountainous areas and from 10 km in lowland areas, and also include information on their main morphological and morphometric characteristics, which were presented only in the 1970s, after the 1970s such extensive research has not been done. The purpose of the study is to identify rivers and temporary streams, as well as to obtain basic hydrographic information through field surveys and with the help of modern GIS technologies. To achieve the goals, complex field work was carried out using modern hydrological and geodetic equipment and interpretation of space images of Sentinel 1,2 satellites using ArcGIS tools and programs. Each river and temporary watercourse record has a unique feature identifier in the geographic information system. The database is intended for use in regional studies in the field of hydrology, ecology, nature management, and is also an information source for the implementation of measures to monitor and restore rivers, organize water use in transboundary river basins.","PeriodicalId":9377,"journal":{"name":"Bulletin of the Karaganda University. “Biology, medicine, geography Series”","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91367087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Woody-shrub vegetation has a key role in greening the urban environment. The excellent decorative qualities of the catalpa are important when choosing a woody plant for introduction in the conditions of the Karaganda region. Seedlings for a particular region must be grown locally to improve their adaptive properties. For a more complete study of the biological features of the species, an anatomical study of young catalpa plants was carried out. It was found that the leaf of the catalpa of the dorsal-ventral type, the stomata of the diacytic type, the cells of the epidermis with winding walls, trichomes and essential-oil glands are located on it; the petiole of the leaf is rounded in shape, with well-developed mechanical tissues around conductive bundles. On the internal structure of the stem and root, the beginning of the formation of cork tissue and the transition to a non-bundle type of structure of the conductive system is observed. In general, the structure of the catalpa is excellent evidence of the mesoxerophyte structure of the plant, which shows its demands on humidification conditions. Leaves are light type, that is, plants when planning green construction must be placed in areas with sufficient lighting. The results obtained will be supplemented by the study of anatomical indicators of plants of subsequent years of development
{"title":"Study of the anatomical structure of the aboveground organs of Catalpa speciosa of the 1st year vegetation","authors":"Aidoz Aitymov, Michael Sergeevich Iamburov","doi":"10.31489/2023bmg1/13-21","DOIUrl":"https://doi.org/10.31489/2023bmg1/13-21","url":null,"abstract":"Woody-shrub vegetation has a key role in greening the urban environment. The excellent decorative qualities of the catalpa are important when choosing a woody plant for introduction in the conditions of the Karaganda region. Seedlings for a particular region must be grown locally to improve their adaptive properties. For a more complete study of the biological features of the species, an anatomical study of young catalpa plants was carried out. It was found that the leaf of the catalpa of the dorsal-ventral type, the stomata of the diacytic type, the cells of the epidermis with winding walls, trichomes and essential-oil glands are located on it; the petiole of the leaf is rounded in shape, with well-developed mechanical tissues around conductive bundles. On the internal structure of the stem and root, the beginning of the formation of cork tissue and the transition to a non-bundle type of structure of the conductive system is observed. In general, the structure of the catalpa is excellent evidence of the mesoxerophyte structure of the plant, which shows its demands on humidification conditions. Leaves are light type, that is, plants when planning green construction must be placed in areas with sufficient lighting. The results obtained will be supplemented by the study of anatomical indicators of plants of subsequent years of development","PeriodicalId":9377,"journal":{"name":"Bulletin of the Karaganda University. “Biology, medicine, geography Series”","volume":"395 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77763127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The present study was conducted at an objective study of the seeds of Sudanese grass for cryopreservation and viability of seeds. Laboratory studies were carried out on 4 varieties of Sudanese grass: Nika, Tigai, Novosibirskaya 84, and Alina. The results showed that cryopreservation had a positive effect on germination rates. Thus, in the Tugay variety, the best germination rates of 99 % were observed in the variant with cryopreservation with defrosting of seeds in a water bath. A positive effect was also noted for the variety Novosibirskaya 84, where cryopreservation during defrosting of seeds at room temperature increased germination by 7 % and amounted to 89 %. For varieties Nika and Alina, cryopreservation had the opposite effect, the indicators decreased by 27 %, by 10 %, respectively, compared with control. Under conditions of seed defrosting at room temperature, cryopreservation with ethylene glycol and propylene glycol had a positive effect on the germination of seeds for all varieties, with the exception of the Alina variety, where its germination did not differ significantly from the control. Cryopreservation using ethylene glycol under conditions of seed defrosting at room temperature showed that for the Tugay variety the recommended concentration of the cryoprotectant is 5 % and 10 %. The germination rate was 98 %, and the germination energy was 99 % and 96 %, respectively. Ethylene glycol concentration of 20 % significantly reduced the germination rate and amounted to 47 %. For the Nika variety, we recommend using a concentration of 15 %; but the concentration of 10 %, the germination rate is lower and amounts to 58 %. Almost 100 % germination were observed in the Novosibirskaya 84 variety at concentrations of 5 % and 20 %. Thus, the recommended method for thawing Sudan grass seeds after cryopreservation using ethylene glycol and propylene glycol cryoprotectants is thawing at room temperature. These results can be used to create a cryo collection of Sudan grass seeds.
{"title":"Study the germination of seeds of Sudanese grass (Sorghum × drummondii) treated by ethylene glycol and propylene glycol cryoprotectants","authors":"Aidana Madiyeva, M. Silantyeva","doi":"10.31489/2023bmg1/76-83","DOIUrl":"https://doi.org/10.31489/2023bmg1/76-83","url":null,"abstract":"The present study was conducted at an objective study of the seeds of Sudanese grass for cryopreservation and viability of seeds. Laboratory studies were carried out on 4 varieties of Sudanese grass: Nika, Tigai, Novosibirskaya 84, and Alina. The results showed that cryopreservation had a positive effect on germination rates. Thus, in the Tugay variety, the best germination rates of 99 % were observed in the variant with cryopreservation with defrosting of seeds in a water bath. A positive effect was also noted for the variety Novosibirskaya 84, where cryopreservation during defrosting of seeds at room temperature increased germination by 7 % and amounted to 89 %. For varieties Nika and Alina, cryopreservation had the opposite effect, the indicators decreased by 27 %, by 10 %, respectively, compared with control. Under conditions of seed defrosting at room temperature, cryopreservation with ethylene glycol and propylene glycol had a positive effect on the germination of seeds for all varieties, with the exception of the Alina variety, where its germination did not differ significantly from the control. Cryopreservation using ethylene glycol under conditions of seed defrosting at room temperature showed that for the Tugay variety the recommended concentration of the cryoprotectant is 5 % and 10 %. The germination rate was 98 %, and the germination energy was 99 % and 96 %, respectively. Ethylene glycol concentration of 20 % significantly reduced the germination rate and amounted to 47 %. For the Nika variety, we recommend using a concentration of 15 %; but the concentration of 10 %, the germination rate is lower and amounts to 58 %. Almost 100 % germination were observed in the Novosibirskaya 84 variety at concentrations of 5 % and 20 %. Thus, the recommended method for thawing Sudan grass seeds after cryopreservation using ethylene glycol and propylene glycol cryoprotectants is thawing at room temperature. These results can be used to create a cryo collection of Sudan grass seeds.","PeriodicalId":9377,"journal":{"name":"Bulletin of the Karaganda University. “Biology, medicine, geography Series”","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73801975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-30DOI: 10.31489/2023bmg1/123-129
Z. Sayakova, A. Yeszhanov
Ixiodid mites are vectors of many transmissible infections, including especially dangerous ones. Although the medical and veterinary significance of this group of blood-sucking arthropods can hardly be overestimated, nevertheless, it is known that intensive study of the fauna of ixodid mites in Kazakhstan fell on the Soviet period, and after the collapse of the USSR, studies of the fauna and taxonomy of ixodid mites were only episodic. Today, the fauna of ixodid mites of the Republic of Kazakhstan includes 42 species of these blood-sucking arachnids, while information on some species of mites is often fragmentary. So, as a result of a survey conducted on the territory of the Ile-Balkhash reserve for the flag, the authors collected 65 specimens of ixodid mites in total. In the course of identification, it was possible to establish that collected mites belonged to the genus Rhipicephalus, and upon species differentiation, all the collected mites turned out to be Rh. leporis. Mites concentrated mainly around living quarters, but the largest number was noted near the enclosure where Bukhara deer (Cervus elaphus bactrianus) were kept. Noteworthy there is the fact that earlier these species of mite was not indicated for this territory and was discovered by the authors here for the first time. Obtaining new information about the fauna of ixodid mites living on the territory of the Ile-Balkhash reserve may be important for the planned introduction of the Amur tiger (Panthera tigris tigris), since ixodid mites carry a large number of zoonotic diseases. Thus it will contribute to a comprehensive risk assessment for tiger introductions and may enhance the success of the planned activity.
{"title":"About the finding of mites Rhipicephalus leporis (Arai, Ixodidae) in the southern Balkhash region","authors":"Z. Sayakova, A. Yeszhanov","doi":"10.31489/2023bmg1/123-129","DOIUrl":"https://doi.org/10.31489/2023bmg1/123-129","url":null,"abstract":"Ixiodid mites are vectors of many transmissible infections, including especially dangerous ones. Although the medical and veterinary significance of this group of blood-sucking arthropods can hardly be overestimated, nevertheless, it is known that intensive study of the fauna of ixodid mites in Kazakhstan fell on the Soviet period, and after the collapse of the USSR, studies of the fauna and taxonomy of ixodid mites were only episodic. Today, the fauna of ixodid mites of the Republic of Kazakhstan includes 42 species of these blood-sucking arachnids, while information on some species of mites is often fragmentary. So, as a result of a survey conducted on the territory of the Ile-Balkhash reserve for the flag, the authors collected 65 specimens of ixodid mites in total. In the course of identification, it was possible to establish that collected mites belonged to the genus Rhipicephalus, and upon species differentiation, all the collected mites turned out to be Rh. leporis. Mites concentrated mainly around living quarters, but the largest number was noted near the enclosure where Bukhara deer (Cervus elaphus bactrianus) were kept. Noteworthy there is the fact that earlier these species of mite was not indicated for this territory and was discovered by the authors here for the first time. Obtaining new information about the fauna of ixodid mites living on the territory of the Ile-Balkhash reserve may be important for the planned introduction of the Amur tiger (Panthera tigris tigris), since ixodid mites carry a large number of zoonotic diseases. Thus it will contribute to a comprehensive risk assessment for tiger introductions and may enhance the success of the planned activity.","PeriodicalId":9377,"journal":{"name":"Bulletin of the Karaganda University. “Biology, medicine, geography Series”","volume":"113 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80579215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In 2021, in the course of studying the entomofauna of the Tarbagatai State National Natural Park in the southern spur of the Tarbagatai Ridge, the Karabas Mountains, a female of large-headed fly (Conopidae) of the genus Physocephala was caught on flowers of the common oregano Origanum vulgare L. (family Lamiaceae) which is described in this paper as a new species Ph. zhanylkan sp. n. The female of the described species is close to the female of Ph. zaitzevi Zimina, 1979, from which it differs in the absence of a spot on the vertex, the proportions of the antennae and proboscis, the color of the wings, the color of the pollination of the abdomen, and the size and shape of the theca.
2021年，在卡拉巴山塔尔巴加泰山脊南麓塔尔巴加泰国家自然公园昆虫区系研究过程中，在普通牛至Origanum vulgare L. (Lamiaceae)(本文将其描述为新种Ph. zhanylkan sp. n.)的花朵上捕获了一只大头蝇(Conopidae)属的雌性，该物种的雌性与Ph. zaitzevi Zimina, 1979的雌性接近。它的不同之处在于顶点上没有斑点，触角和喙的比例，翅膀的颜色，腹部授粉的颜色，以及卵膜的大小和形状。
{"title":"New species of genus Physocephala Schiner, 1861 (Diptera, Conopidae) from Eastern Kazakhstan","authors":"Boris Vasiljevich Zlatanov","doi":"10.31489/2023bmg1/56-60","DOIUrl":"https://doi.org/10.31489/2023bmg1/56-60","url":null,"abstract":"In 2021, in the course of studying the entomofauna of the Tarbagatai State National Natural Park in the southern spur of the Tarbagatai Ridge, the Karabas Mountains, a female of large-headed fly (Conopidae) of the genus Physocephala was caught on flowers of the common oregano Origanum vulgare L. (family Lamiaceae) which is described in this paper as a new species Ph. zhanylkan sp. n. The female of the described species is close to the female of Ph. zaitzevi Zimina, 1979, from which it differs in the absence of a spot on the vertex, the proportions of the antennae and proboscis, the color of the wings, the color of the pollination of the abdomen, and the size and shape of the theca.","PeriodicalId":9377,"journal":{"name":"Bulletin of the Karaganda University. “Biology, medicine, geography Series”","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78641297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-30DOI: 10.31489/2023bmg1/110-116
A. B. Myrzabaev, A. Amanzholov, D. Golovanov, M. Bodeyev, Jacek Urbanіak
The article presents the results of the work carried out on the analysis of factors and processes of soil formation of the Karkaraly State National Natural Park. In the studied area of the soil cover of the national park, it was noted that they develop due to two main factors — vertical zoning and geological specificity of the region. The soils of the area consist of granite, diorite and metamorphic dense rocks. All ancient rocks are covered with quaternary deposits of varying thickness. The work done began with the preparation of routes to control points and a research program. After that, work was carried out on the study of control points: the creation of soil sections — the description of morphological properties of soils, obtaining soil samples along the horizons. The field work was carried out in forest, mountain-forest and steppe landscapes. According to the soil sections of the test points in these places, their color was determined on the Mansell scale. The moisture and granulometric composition of the soil and the boiling of hydrogen chloride were determined.
{"title":"Factors and processes of soil formation of the Karkaraly State National Natural Park","authors":"A. B. Myrzabaev, A. Amanzholov, D. Golovanov, M. Bodeyev, Jacek Urbanіak","doi":"10.31489/2023bmg1/110-116","DOIUrl":"https://doi.org/10.31489/2023bmg1/110-116","url":null,"abstract":"The article presents the results of the work carried out on the analysis of factors and processes of soil formation of the Karkaraly State National Natural Park. In the studied area of the soil cover of the national park, it was noted that they develop due to two main factors — vertical zoning and geological specificity of the region. The soils of the area consist of granite, diorite and metamorphic dense rocks. All ancient rocks are covered with quaternary deposits of varying thickness. The work done began with the preparation of routes to control points and a research program. After that, work was carried out on the study of control points: the creation of soil sections — the description of morphological properties of soils, obtaining soil samples along the horizons. The field work was carried out in forest, mountain-forest and steppe landscapes. According to the soil sections of the test points in these places, their color was determined on the Mansell scale. The moisture and granulometric composition of the soil and the boiling of hydrogen chloride were determined.","PeriodicalId":9377,"journal":{"name":"Bulletin of the Karaganda University. “Biology, medicine, geography Series”","volume":"90 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87118215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Mit, O. Cherednichenko, A. Musaeva, Ozada Khakimovna Khamdieva, A.S. Amirgalieva, M. Begmanova, A. Tolebaeva, A. L. Pilyugina, S. Nuraliev, Saule Zaypanova, Gulshat Koishekenova, Aidana Kuandykovna Bekitaeva, Tilek Kapasuly
Obsolete organochlorine pesticides are currently prohibited as persistent organic pollutants. If undisposed they continue to pollute soil and water, to accumulate in the food chain and to harm plants, animals and human body. The aim of the study was to assess the pollution of water and soil around the warehouses by unutilized banned organochlorine pesticides and their possible genotoxic effects. Former warehouses of pesticides were investigated in two settlements of Zhambyl district of Almaty region. Chemical analysis showed contamination of the soil around the warehouses with organochlorine pesticides and heavy metals. Water and soil samples taken near the storage facilities were used for environmental risk assessment. The genotoxic effect of water and soil samples was evaluated on various model objects: Salmonella typhymurium, Drosophila melanogaster, Allium cepa, sheep and human lymphocyte cultures. It was found that water and soil samples caused a weak mutagenic effect in all model systems, increasing the frequency of mutations and chromosomal aberrations. Although a genotoxic effect was demonstrated for each of models, different models showed different sensitivity to the action of pesticides and varied degrees of response. Therefore, for adequate assessment of the mutagenicity of pesticides, it is necessary to use a battery of tests with different model objects. The results demonstrated that obsolete pesticide residues still pollute the environment and must be disposed of in a safe way in a timely manner to avoid environmental pollution.
{"title":"Evaluation of the mutagenic effect of water and soil samples collected near the former storage facilities for organochlorine pesticides in the Zhambyl district of the Almaty region using various model test systems","authors":"N. Mit, O. Cherednichenko, A. Musaeva, Ozada Khakimovna Khamdieva, A.S. Amirgalieva, M. Begmanova, A. Tolebaeva, A. L. Pilyugina, S. Nuraliev, Saule Zaypanova, Gulshat Koishekenova, Aidana Kuandykovna Bekitaeva, Tilek Kapasuly","doi":"10.31489/2023bmg1/84-97","DOIUrl":"https://doi.org/10.31489/2023bmg1/84-97","url":null,"abstract":"Obsolete organochlorine pesticides are currently prohibited as persistent organic pollutants. If undisposed they continue to pollute soil and water, to accumulate in the food chain and to harm plants, animals and human body. The aim of the study was to assess the pollution of water and soil around the warehouses by unutilized banned organochlorine pesticides and their possible genotoxic effects. Former warehouses of pesticides were investigated in two settlements of Zhambyl district of Almaty region. Chemical analysis showed contamination of the soil around the warehouses with organochlorine pesticides and heavy metals. Water and soil samples taken near the storage facilities were used for environmental risk assessment. The genotoxic effect of water and soil samples was evaluated on various model objects: Salmonella typhymurium, Drosophila melanogaster, Allium cepa, sheep and human lymphocyte cultures. It was found that water and soil samples caused a weak mutagenic effect in all model systems, increasing the frequency of mutations and chromosomal aberrations. Although a genotoxic effect was demonstrated for each of models, different models showed different sensitivity to the action of pesticides and varied degrees of response. Therefore, for adequate assessment of the mutagenicity of pesticides, it is necessary to use a battery of tests with different model objects. The results demonstrated that obsolete pesticide residues still pollute the environment and must be disposed of in a safe way in a timely manner to avoid environmental pollution.","PeriodicalId":9377,"journal":{"name":"Bulletin of the Karaganda University. “Biology, medicine, geography Series”","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77634493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-30DOI: 10.31489/2023bmg1/167-174
A. K. Sarkytbayeva, S. Kubentayev, M. Kurmanbayeva, G. Aidarkhanova, Zhanar Iskakova, Moldir Z. Zhumagul, A. Kusmangazinov
Paeonia anomala L. is a valuable medicinal plant in the world. The aim of this study was the study of composition of the plant P. anomala and its antioxidant, antiradical and cytotoxic activities. The underground part of P. anomala was extracted with ethanol and the composition was determined using gas chromatography with mass spectrometric detection (Agilent 7890A/5975C). Methyl salicylate (32.07 %), sucrose (11.23 %), benzoic acid (9.12 %), cyclopropyl carbine (7.7 %), 2,5-dimethyl-4-hydroxy-3(2H)-furanone (4.77 %), benzoic acid, 2,4-dihydroxy-3,5,6-trimethyl-, methyl ester (4.48 %), are the main components. The ethyl acetate extract of the underground part (root) of P. anomala at concentrations of 0.25 and 0.5 mg/ml has a low content, and at concentrations of 0.75 and 1 mg/ml, an average antioxidant activity compared to the antioxidant activity of ascorbic and gallic acids. The ethanol extract of the underground part of P. anomala at concentrations of 0.25 and 0.5 mg/ml has an average, and at concentrations of 0.75 and 1 mg/ml the highest antioxidant activity compared to the antioxidant activity comparators. Also, the ethanol extract of the underground part P. anomala has the highest antiradical activity in all concentrations compared to the antiradical activity of gallic acid. Ethanol and ethyl acetate extracts of the underground part of P. anomala in all concentrations do not show cytotoxicity.
{"title":"Paeonia anomala (Paeoniaceae) component composition and antioxidant, antiradical and cytotoxic activity","authors":"A. K. Sarkytbayeva, S. Kubentayev, M. Kurmanbayeva, G. Aidarkhanova, Zhanar Iskakova, Moldir Z. Zhumagul, A. Kusmangazinov","doi":"10.31489/2023bmg1/167-174","DOIUrl":"https://doi.org/10.31489/2023bmg1/167-174","url":null,"abstract":"Paeonia anomala L. is a valuable medicinal plant in the world. The aim of this study was the study of composition of the plant P. anomala and its antioxidant, antiradical and cytotoxic activities. The underground part of P. anomala was extracted with ethanol and the composition was determined using gas chromatography with mass spectrometric detection (Agilent 7890A/5975C). Methyl salicylate (32.07 %), sucrose (11.23 %), benzoic acid (9.12 %), cyclopropyl carbine (7.7 %), 2,5-dimethyl-4-hydroxy-3(2H)-furanone (4.77 %), benzoic acid, 2,4-dihydroxy-3,5,6-trimethyl-, methyl ester (4.48 %), are the main components. The ethyl acetate extract of the underground part (root) of P. anomala at concentrations of 0.25 and 0.5 mg/ml has a low content, and at concentrations of 0.75 and 1 mg/ml, an average antioxidant activity compared to the antioxidant activity of ascorbic and gallic acids. The ethanol extract of the underground part of P. anomala at concentrations of 0.25 and 0.5 mg/ml has an average, and at concentrations of 0.75 and 1 mg/ml the highest antioxidant activity compared to the antioxidant activity comparators. Also, the ethanol extract of the underground part P. anomala has the highest antiradical activity in all concentrations compared to the antiradical activity of gallic acid. Ethanol and ethyl acetate extracts of the underground part of P. anomala in all concentrations do not show cytotoxicity.","PeriodicalId":9377,"journal":{"name":"Bulletin of the Karaganda University. “Biology, medicine, geography Series”","volume":"57 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91075884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Bisseneva, G. Pogossyan, Constantin Grigoryevich Li
The article presents the results of genotyping of DNA samples obtained from study participants with the established status of coronavirus infection (COVID-19) using enzyme immunoassay for the single nucleotide polymorphism rs12329760 (C/T) of the TMPRSS2 gene. Genotyping was carried out by polymerase chain reaction (PCR) in real time using the technique “Amplification of the refractory mutation system” (ARMS). The distribution of frequencies of genotypes and alleles rs12329760 C>T of the TMPRSS2 gene in 80 people of the experimental and control groups was analyzed. The presence of the significance of the single nucleotide polymorphism rs12329760 of the TMPRSS2 gene in the homozygous state (CC) and heterozygous (CT) and the absence of the significance of the TT genotype was found. A statistically significant difference in the distribution of the T allele was found.
{"title":"Association of polymorphism rs12329760 of the TMPRSS2 gene with coronavirus infection","authors":"A. Bisseneva, G. Pogossyan, Constantin Grigoryevich Li","doi":"10.31489/2023bmg1/44-48","DOIUrl":"https://doi.org/10.31489/2023bmg1/44-48","url":null,"abstract":"The article presents the results of genotyping of DNA samples obtained from study participants with the established status of coronavirus infection (COVID-19) using enzyme immunoassay for the single nucleotide polymorphism rs12329760 (C/T) of the TMPRSS2 gene. Genotyping was carried out by polymerase chain reaction (PCR) in real time using the technique “Amplification of the refractory mutation system” (ARMS). The distribution of frequencies of genotypes and alleles rs12329760 C>T of the TMPRSS2 gene in 80 people of the experimental and control groups was analyzed. The presence of the significance of the single nucleotide polymorphism rs12329760 of the TMPRSS2 gene in the homozygous state (CC) and heterozygous (CT) and the absence of the significance of the TT genotype was found. A statistically significant difference in the distribution of the T allele was found.","PeriodicalId":9377,"journal":{"name":"Bulletin of the Karaganda University. “Biology, medicine, geography Series”","volume":"96 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74836974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gauhar Askarovna Amertayeva, A. Mamurova, B. Osmonali
Medicinal plants are currently the most relevant objects of research. Since the final product that is obtained from useful plants belongs to the most valuable medicines. Particularly interesting plants in medicine are those containing essential oils, for example, species of the genus Artemisia, but few people know that closely related species of the genus Ajania are also rich in essential oils. The genus Ajania belongs to the Asteraceae family. Only 2 species of this genus are found in Kazakhstan: Ajania fruticulosa (Ledeb.) Poljak (shrub) and Ajania fastigiata (C. Winkl.) Poljak (perennial plant).Our object of research is Ajania fastigiata, which is distributed throughout the mountainous territory of the Southern and Southeastern regions of the Republic of Kazakhstan. It is little studied in terms of precise morphological and anatomical features, knowledge of which makes it possible to quickly determine and refine the species. The nearest point in the Trans-Ili Alatau was chosen for the prototype. The research methods were classical botanical and anatomical. When analyzing the approximate population, the morphological data of the species for this territory were clarified. Anatomical studies have shown that the leaves of Ajania fastigiata contain essential oil glands and receptacles for metabolic products. The stems of this species have a characteristic arrangement of sclerenchyma in separate strands.
{"title":"Morphological characteristics and anatomical features of the perennial herb Ajania fastigiata (C. Winkl.) Poljakov (Asteraceae)","authors":"Gauhar Askarovna Amertayeva, A. Mamurova, B. Osmonali","doi":"10.31489/2023bmg1/28-36","DOIUrl":"https://doi.org/10.31489/2023bmg1/28-36","url":null,"abstract":"Medicinal plants are currently the most relevant objects of research. Since the final product that is obtained from useful plants belongs to the most valuable medicines. Particularly interesting plants in medicine are those containing essential oils, for example, species of the genus Artemisia, but few people know that closely related species of the genus Ajania are also rich in essential oils. The genus Ajania belongs to the Asteraceae family. Only 2 species of this genus are found in Kazakhstan: Ajania fruticulosa (Ledeb.) Poljak (shrub) and Ajania fastigiata (C. Winkl.) Poljak (perennial plant).Our object of research is Ajania fastigiata, which is distributed throughout the mountainous territory of the Southern and Southeastern regions of the Republic of Kazakhstan. It is little studied in terms of precise morphological and anatomical features, knowledge of which makes it possible to quickly determine and refine the species. The nearest point in the Trans-Ili Alatau was chosen for the prototype. The research methods were classical botanical and anatomical. When analyzing the approximate population, the morphological data of the species for this territory were clarified. Anatomical studies have shown that the leaves of Ajania fastigiata contain essential oil glands and receptacles for metabolic products. The stems of this species have a characteristic arrangement of sclerenchyma in separate strands.","PeriodicalId":9377,"journal":{"name":"Bulletin of the Karaganda University. “Biology, medicine, geography Series”","volume":"133 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76785804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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