The article presents the results of screening lactic acid bacteria cultures of the genus Lactobacillus and Pediococcus from the collection of Kazakh Scientific Research Institute of Processing and Food Industry, previously isolated from wheat grain, flour, rye starter cultures, as well as kumis and shubat. For a comprehensive assessment of probiotic properties of the selected 7 strains of lactic acid bacteria, their physiological and biochemical properties were studied: acid-forming, antagonistic activity, saccharolytic profile, resistance to various concentrations of bile, sodium chloride, growth at different pH values, antibiotic resistance. As a result of strains screening 5 most active cultures of lactic acid bacteria were selected for inclusion in the sourdough for bread: Limosilactobacillus pontis 9K3, Limosilactobacillus fermentum 3Ш1, Lb. paracasei 82, Lb. paracasei 114, Lacticaseibacillus paracasei 126.The selection criteria were high enzymatic, acid-forming, proteolytic and antagonistic activity of the strains against opportunistic and pathogenic microflora (B. subtilis ATCC 6633, Escherichia coli-1257, Staphylococcus sp.209-P, Salmonella Typhimurium). We show that this result of the analysis indicates the prospects of using a consortium of these cultures to obtain a probiotic starter culture for bakery products. This, in turn, will increase production efficiency, ensure the quality and safety of food products in the baking industry.
{"title":"Studying the physiological and biochemical properties of lactic bacteria cultures in the creation of sourdough for bread","authors":"Moldir Sabetkyzy Isabekova, L. Umiraliyeva","doi":"10.31489/2022bmg4/60-67","DOIUrl":"https://doi.org/10.31489/2022bmg4/60-67","url":null,"abstract":"The article presents the results of screening lactic acid bacteria cultures of the genus Lactobacillus and Pediococcus from the collection of Kazakh Scientific Research Institute of Processing and Food Industry, previously isolated from wheat grain, flour, rye starter cultures, as well as kumis and shubat. For a comprehensive assessment of probiotic properties of the selected 7 strains of lactic acid bacteria, their physiological and biochemical properties were studied: acid-forming, antagonistic activity, saccharolytic profile, resistance to various concentrations of bile, sodium chloride, growth at different pH values, antibiotic resistance. As a result of strains screening 5 most active cultures of lactic acid bacteria were selected for inclusion in the sourdough for bread: Limosilactobacillus pontis 9K3, Limosilactobacillus fermentum 3Ш1, Lb. paracasei 82, Lb. paracasei 114, Lacticaseibacillus paracasei 126.The selection criteria were high enzymatic, acid-forming, proteolytic and antagonistic activity of the strains against opportunistic and pathogenic microflora (B. subtilis ATCC 6633, Escherichia coli-1257, Staphylococcus sp.209-P, Salmonella Typhimurium). We show that this result of the analysis indicates the prospects of using a consortium of these cultures to obtain a probiotic starter culture for bakery products. This, in turn, will increase production efficiency, ensure the quality and safety of food products in the baking industry.","PeriodicalId":9377,"journal":{"name":"Bulletin of the Karaganda University. “Biology, medicine, geography Series”","volume":"105 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90672122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Z. Kuanbay, Gulnur Bulatovna Admanova, Sardarbek Abiyevich Abiуev, L. S. Kozhamzharova
The article discusses the distribution of the Amaranthaceae family of the Donyztau chunk in the Aktobe region. In the Donyztau flora, 25 genera have been registered, which combine 67 species. The Amaranthaceae family occupies the first place in the species of the Donyztau flora, making up 20 % of the entire flora. Taxonomic analysis showed that Salsola, Anabasis, Suaeda, Climacoptera, and Atriplex belong to the dominant genera in the family. The article provides a summary of the species of the family. An ecobiomorphological analysis of the species composition of the Amaranthaceae family depending on the substrate, was carried out. Analysis of the life forms is developed according to the classification of I.G. Serebryakov, ecological analysis — according to the classification of A.P. Shennikov. The analysis of life forms displayed the predominance of annual species. Ecological analysis has proved that the vast majority of species belong to xerophiles. Their share is 80 %. The article provides a synopsis of the species of the genus. The summary reflected the Latin-Kazakh names of species, life forms, ecological groups associated with the substrate, economic significance and area. Some species play an important role as an edifier.
{"title":"The Amaranthaceae family in the flora of Donyztau","authors":"Z. Kuanbay, Gulnur Bulatovna Admanova, Sardarbek Abiyevich Abiуev, L. S. Kozhamzharova","doi":"10.31489/2022bmg4/76-82","DOIUrl":"https://doi.org/10.31489/2022bmg4/76-82","url":null,"abstract":"The article discusses the distribution of the Amaranthaceae family of the Donyztau chunk in the Aktobe region. In the Donyztau flora, 25 genera have been registered, which combine 67 species. The Amaranthaceae family occupies the first place in the species of the Donyztau flora, making up 20 % of the entire flora. Taxonomic analysis showed that Salsola, Anabasis, Suaeda, Climacoptera, and Atriplex belong to the dominant genera in the family. The article provides a summary of the species of the family. An ecobiomorphological analysis of the species composition of the Amaranthaceae family depending on the substrate, was carried out. Analysis of the life forms is developed according to the classification of I.G. Serebryakov, ecological analysis — according to the classification of A.P. Shennikov. The analysis of life forms displayed the predominance of annual species. Ecological analysis has proved that the vast majority of species belong to xerophiles. Their share is 80 %. The article provides a synopsis of the species of the genus. The summary reflected the Latin-Kazakh names of species, life forms, ecological groups associated with the substrate, economic significance and area. Some species play an important role as an edifier.","PeriodicalId":9377,"journal":{"name":"Bulletin of the Karaganda University. “Biology, medicine, geography Series”","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73141453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Currently, active research work is underway to study and use site-specific RNA-guided endonucleases as tools for use in the field of genome editing and diagnostics in the biomedical and biotechnological fields. To date, the most effective method in this area is the CRISPR method. Due to its ease of targeting, this system was quickly adopted as the method of choice for editing the genomes of numerous organisms. More recently, another novel CRISPR-Cas class 2 endonuclease with characteristic features has been discovered in bacterial genomes: Cas12a. The Cas12a enzyme is a site-specific RNA-guided endonuclease that can be used for precise genome editing in various cell types of different species, as well as for diagnostic applications. The search, identification and characterization of new unexplored homologues will expand the potential of enzyme applications. In this work, the expression and two-stage chromatographic purification of the recombinant enzyme Cas12a of high purity were carried out. In vitro synthesized crRNA, ribonucleoprotein complex were obtained and by the endonuclease activity of the enzyme in relation to the substrate containing the target sequence for cleavage in the appropriate site was confirmed. The resulting enzyme can be used to further describe its kinetic parameters, which can be applied in the development of new next-generation diagnostics.
{"title":"Expression and purification of recombinant DNA endonuclease of CRISPR/Cas system","authors":"M. Amanzholova, A. Shaizadinova, S. Abeldenov","doi":"10.31489/2022bmg4/7-13","DOIUrl":"https://doi.org/10.31489/2022bmg4/7-13","url":null,"abstract":"Currently, active research work is underway to study and use site-specific RNA-guided endonucleases as tools for use in the field of genome editing and diagnostics in the biomedical and biotechnological fields. To date, the most effective method in this area is the CRISPR method. Due to its ease of targeting, this system was quickly adopted as the method of choice for editing the genomes of numerous organisms. More recently, another novel CRISPR-Cas class 2 endonuclease with characteristic features has been discovered in bacterial genomes: Cas12a. The Cas12a enzyme is a site-specific RNA-guided endonuclease that can be used for precise genome editing in various cell types of different species, as well as for diagnostic applications. The search, identification and characterization of new unexplored homologues will expand the potential of enzyme applications. In this work, the expression and two-stage chromatographic purification of the recombinant enzyme Cas12a of high purity were carried out. In vitro synthesized crRNA, ribonucleoprotein complex were obtained and by the endonuclease activity of the enzyme in relation to the substrate containing the target sequence for cleavage in the appropriate site was confirmed. The resulting enzyme can be used to further describe its kinetic parameters, which can be applied in the development of new next-generation diagnostics.","PeriodicalId":9377,"journal":{"name":"Bulletin of the Karaganda University. “Biology, medicine, geography Series”","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76678812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-30DOI: 10.31489/2022bmg4/140-147
M. Tynykulov
Perpetual raspberry is a group of raspberry varieties distinguished by their ability to bear fruit on two-year and one-year stem shoots. Black currant (Ríbes nígrum) is a deciduous shrub, family Gooseberry (Grossulariaceae), related to currants (Ribes). The article presents methods of cell selection of perpetual raspberries and currants. Crop varieties resistant to extreme natural factors, obtaining high harvest, and two harvests per year were considered. The chemical composition of the used nutrient medium, the content of micro- and macroelements, and organic substances were taken into account. The qualitative composition of callus shoots extracted from plant cells was determined. A strong relationship between the frequency of callusogenesis in black currant and the balance of phytohormones in the nutrient medium has been shown. Progress in the field of gene and cell biotechnology is directly related to the development of the basics of the cultivation of plant cells and tissues under in vitro conditions. In addition, it is of particular interest to choose a suitable nutrient medium, to establish the mode of cultivation in order to determine the species and volume of explants, the totipotency of representatives of different taxonomic groups of plants. At present, this unique ability of somatic cells is found in various cultures, most of which are annual and practically vegetative propagating plants. These are vegetable crops and ornamental plants, the list of which grows annually. The scientific novelty of the work is the possibility of obtaining somatic hybrids and transgenic plants of repair raspberry and black currant using biotechnology methods. Therefore, the study of theoretical foundations and development of applied aspects of cells and tissues of perennial plants, in particular fruit and berry and agricultural crops, their accelerated reproduction, obtaining somatic hybrids and transgenic plants are considered relevant.
{"title":"Peculiarity of currant and perpetual repair cell culture","authors":"M. Tynykulov","doi":"10.31489/2022bmg4/140-147","DOIUrl":"https://doi.org/10.31489/2022bmg4/140-147","url":null,"abstract":"Perpetual raspberry is a group of raspberry varieties distinguished by their ability to bear fruit on two-year and one-year stem shoots. Black currant (Ríbes nígrum) is a deciduous shrub, family Gooseberry (Grossulariaceae), related to currants (Ribes). The article presents methods of cell selection of perpetual raspberries and currants. Crop varieties resistant to extreme natural factors, obtaining high harvest, and two harvests per year were considered. The chemical composition of the used nutrient medium, the content of micro- and macroelements, and organic substances were taken into account. The qualitative composition of callus shoots extracted from plant cells was determined. A strong relationship between the frequency of callusogenesis in black currant and the balance of phytohormones in the nutrient medium has been shown. Progress in the field of gene and cell biotechnology is directly related to the development of the basics of the cultivation of plant cells and tissues under in vitro conditions. In addition, it is of particular interest to choose a suitable nutrient medium, to establish the mode of cultivation in order to determine the species and volume of explants, the totipotency of representatives of different taxonomic groups of plants. At present, this unique ability of somatic cells is found in various cultures, most of which are annual and practically vegetative propagating plants. These are vegetable crops and ornamental plants, the list of which grows annually. The scientific novelty of the work is the possibility of obtaining somatic hybrids and transgenic plants of repair raspberry and black currant using biotechnology methods. Therefore, the study of theoretical foundations and development of applied aspects of cells and tissues of perennial plants, in particular fruit and berry and agricultural crops, their accelerated reproduction, obtaining somatic hybrids and transgenic plants are considered relevant.","PeriodicalId":9377,"journal":{"name":"Bulletin of the Karaganda University. “Biology, medicine, geography Series”","volume":"112 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85015532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-30DOI: 10.31489/2022bmg4/101-106
K. A. Nurlybaeva, Gulnar Zhanatovna Mukasheva, Aiman Shaimenovna Sarsembaeva, Rakhat T. Bodeeva
The article discussed the situation of atmospheric air pollution in the city of Balkhash with chemical elements. The size of suspended particles according to the results of stationary observation of the RM-10 sample was 0.8 MPC with a concentration of dust particles (dust/aerosol, not differentiated by composition) of 99.6%. The norm is 0.05 mg/m3 , the maximum single concentration of solid particles in the atmospheric air of the city of Balkhash was 0.2 MPC. The size of RM is 2.5 concentration of suspended particles. According to the norms, the average daily level of RM 2.5 should not exceed 0.25 mg/m3. Based on our data, the average daily level of RM 2.5 was the maximum RM-2.5 - 0.2 MPC of a single concentration of solid particles with a multiplicity exceeding 0.7 mg/m3 . The average concentration of ozone in the whole city is 1.5 MPC s.s. sulfur dioxide -5.4 MPC m.r.k.; carbon monoxide-2.2 MPC m.r.k; nitrogen dioxide -2.0 MPC m.r.k.; hydrogen sulfide - 20.1 mpc.R.K. It was found that at the maximum permissible single concentration of 0.5 mg/m3, the actual concentration of sulfur dioxide during adverse meteorological conditions exceeded the corresponding MPC by 23.7 times.
{"title":"Pollution of the atmospheric air of the city of Balkhash with heavy metals","authors":"K. A. Nurlybaeva, Gulnar Zhanatovna Mukasheva, Aiman Shaimenovna Sarsembaeva, Rakhat T. Bodeeva","doi":"10.31489/2022bmg4/101-106","DOIUrl":"https://doi.org/10.31489/2022bmg4/101-106","url":null,"abstract":"The article discussed the situation of atmospheric air pollution in the city of Balkhash with chemical elements. The size of suspended particles according to the results of stationary observation of the RM-10 sample was 0.8 MPC with a concentration of dust particles (dust/aerosol, not differentiated by composition) of 99.6%. The norm is 0.05 mg/m3 , the maximum single concentration of solid particles in the atmospheric air of the city of Balkhash was 0.2 MPC. The size of RM is 2.5 concentration of suspended particles. According to the norms, the average daily level of RM 2.5 should not exceed 0.25 mg/m3. Based on our data, the average daily level of RM 2.5 was the maximum RM-2.5 - 0.2 MPC of a single concentration of solid particles with a multiplicity exceeding 0.7 mg/m3 . The average concentration of ozone in the whole city is 1.5 MPC s.s. sulfur dioxide -5.4 MPC m.r.k.; carbon monoxide-2.2 MPC m.r.k; nitrogen dioxide -2.0 MPC m.r.k.; hydrogen sulfide - 20.1 mpc.R.K. It was found that at the maximum permissible single concentration of 0.5 mg/m3, the actual concentration of sulfur dioxide during adverse meteorological conditions exceeded the corresponding MPC by 23.7 times.","PeriodicalId":9377,"journal":{"name":"Bulletin of the Karaganda University. “Biology, medicine, geography Series”","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85676626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-30DOI: 10.31489/2022bmg4/164-172
S. Akhmetova, I. Belyayev, A. Omarova, Zhanerke Amirkhanova
In modern conditions, the quality of water supply and the state of water management facilities play a key role in maintaining the health of people and animals, as well as the life quality of the population. In this regard, we set the goal of our research to assess the level of microbial contamination of water and sanitary facilities in the Regional Clinical Hospital of Karaganda district as the central and most powerful medical institution in the region in different seasons of the year. Based on the results of our study, the following conclusions can be drawn: during the research period, seasonal variability of indicators in the studied samples of tap water can be traced. The water of the central water supply is characterized by systematic excesses of both total coliform and thermotolerant coliform bacteria. In the city of Karaganda, there is a great stability in the quality of water supply, regardless of the seasons, despite the excess of standard indicators. It can talk about either the low quality of water treatment, or about quality problems in the water supply network at the sampling points (Regional Clinical Hospital of Karaganda). At the same time, it is worth noting the high percentage (almost 98 %) of positive samples in the spring. In winter, there is a lower percentage of positive samples and a significantly lower microbial quantity. The quality of drinking water decreases in the spring, we believe that the most likely reason for the deterioration in the quality of drinking water is the ingress of melt water and rainwater into the source. Violations of the technological conditions for the operation of water treatment facilities, secondary water pollution in worn-out water distribution networks could also be the cause of unsatisfactory results. In the Regional Clinical Hospital of Karaganda, a higher percentage of positive swabs is noted, which is associated with a higher load on the regional hospital, as well as a better supply of antimicrobial disinfectants, which leads to selective selection of more resistant strains.
{"title":"The study of microbial contamination of water and sanitary facilities in the Karaganda regional clinical hospital during different seasons","authors":"S. Akhmetova, I. Belyayev, A. Omarova, Zhanerke Amirkhanova","doi":"10.31489/2022bmg4/164-172","DOIUrl":"https://doi.org/10.31489/2022bmg4/164-172","url":null,"abstract":"In modern conditions, the quality of water supply and the state of water management facilities play a key role in maintaining the health of people and animals, as well as the life quality of the population. In this regard, we set the goal of our research to assess the level of microbial contamination of water and sanitary facilities in the Regional Clinical Hospital of Karaganda district as the central and most powerful medical institution in the region in different seasons of the year. Based on the results of our study, the following conclusions can be drawn: during the research period, seasonal variability of indicators in the studied samples of tap water can be traced. The water of the central water supply is characterized by systematic excesses of both total coliform and thermotolerant coliform bacteria. In the city of Karaganda, there is a great stability in the quality of water supply, regardless of the seasons, despite the excess of standard indicators. It can talk about either the low quality of water treatment, or about quality problems in the water supply network at the sampling points (Regional Clinical Hospital of Karaganda). At the same time, it is worth noting the high percentage (almost 98 %) of positive samples in the spring. In winter, there is a lower percentage of positive samples and a significantly lower microbial quantity. The quality of drinking water decreases in the spring, we believe that the most likely reason for the deterioration in the quality of drinking water is the ingress of melt water and rainwater into the source. Violations of the technological conditions for the operation of water treatment facilities, secondary water pollution in worn-out water distribution networks could also be the cause of unsatisfactory results. In the Regional Clinical Hospital of Karaganda, a higher percentage of positive swabs is noted, which is associated with a higher load on the regional hospital, as well as a better supply of antimicrobial disinfectants, which leads to selective selection of more resistant strains.","PeriodicalId":9377,"journal":{"name":"Bulletin of the Karaganda University. “Biology, medicine, geography Series”","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76720964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-30DOI: 10.31489/2022bmg4/118-125
M. Sagyndykova, A. Imanbayeva, G. Gassanova
The study of medicinal plant resources is important for the organization of a system of rational environmental management and environmental monitoring. Resource surveys of the natural territories of the Atyrau region were carried out to determine the potential for procurement of medicinal raw materials. Thickets suitable for harvesting were identified for 10 species of medicinal plants. The localization sites of thickets, the yield of plant raw materials (for air-dry weight), abundance and operational reserves were determined. The following areas of thickets and volumes of potential collection of raw materials were established: Peganum harmala — 22.5 hectares and 116.95 tons; Alhagi pseudoalhagi — 228.0 hectares and 196.05 tons; Alhagi persarum — 12.3 hectares and 13.21 tons; Scabiosa isetensis — 12.3 hectares and 4.2 tons; Artemisia terrae-albae — 40.2 hectares and 13.43 tons; Tanacetum santolina — 2.3 hectares and 0.9 tons; Helichrysum arenarium — 15.4 hectares and 2.1 tons; Anabasis aphylla — 126.0 hectares and 24.89 tons; Glycyrrhiza glabra — 36.2 hectares and 20.48 tons; Althaea officinalis — 88.0 hectares and 14.24 tons. The obtained information on plant resources can be used in planning and organizing raw materials for the needs of the pharmaceutical industry of Kazakhstan.
{"title":"To the study of resources of the medicinal plants of the Atyrau region","authors":"M. Sagyndykova, A. Imanbayeva, G. Gassanova","doi":"10.31489/2022bmg4/118-125","DOIUrl":"https://doi.org/10.31489/2022bmg4/118-125","url":null,"abstract":"The study of medicinal plant resources is important for the organization of a system of rational environmental management and environmental monitoring. Resource surveys of the natural territories of the Atyrau region were carried out to determine the potential for procurement of medicinal raw materials. Thickets suitable for harvesting were identified for 10 species of medicinal plants. The localization sites of thickets, the yield of plant raw materials (for air-dry weight), abundance and operational reserves were determined. The following areas of thickets and volumes of potential collection of raw materials were established: Peganum harmala — 22.5 hectares and 116.95 tons; Alhagi pseudoalhagi — 228.0 hectares and 196.05 tons; Alhagi persarum — 12.3 hectares and 13.21 tons; Scabiosa isetensis — 12.3 hectares and 4.2 tons; Artemisia terrae-albae — 40.2 hectares and 13.43 tons; Tanacetum santolina — 2.3 hectares and 0.9 tons; Helichrysum arenarium — 15.4 hectares and 2.1 tons; Anabasis aphylla — 126.0 hectares and 24.89 tons; Glycyrrhiza glabra — 36.2 hectares and 20.48 tons; Althaea officinalis — 88.0 hectares and 14.24 tons. The obtained information on plant resources can be used in planning and organizing raw materials for the needs of the pharmaceutical industry of Kazakhstan.","PeriodicalId":9377,"journal":{"name":"Bulletin of the Karaganda University. “Biology, medicine, geography Series”","volume":"529 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80159352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Karamendin, A. Kydyrmanov, A. Seidalina, Zhumagul Kirkimbaeva, Sattar Turashuli Ozgan, S. Fereidouni
An outbreak of a bacterial disease with lethal cases was registered in camels in Kazakhstan in 2018. The disease was characterised by dry, harsh hacking cough and nasal discharge with froathy and bloody liquid. Camels died in a state of hyperexcitement and asphyxia. Investigation of the case has identified the pathogenic form of Paeniclostridium sordellii as the main cause of mortality in camels. The purpose of the study is to identify the cause of mortality among camels, bacteriological and molecular genetic characteristics of the pathogen. The methodological basis of the work is collection of biological samples from camels, isolating the pathogen on media, extracting nucleic acids, obtaining libraries for next generation sequencing, bioinformatic analysis of the obtained data, pathogenicity test on mice. This article describes the cultural, genetic and phenotypic characteristics of the isolated Shetpe PS18-01 strain. It was shown that the pathogenesis was mainly characterised by respiratory signs, in contrast to the most common gastrointestinal ones.
{"title":"Fatal outbreak among camels in Kazakhstan associated with Paeniclostridium sordellii","authors":"K. Karamendin, A. Kydyrmanov, A. Seidalina, Zhumagul Kirkimbaeva, Sattar Turashuli Ozgan, S. Fereidouni","doi":"10.31489/2022bmg4/68-75","DOIUrl":"https://doi.org/10.31489/2022bmg4/68-75","url":null,"abstract":"An outbreak of a bacterial disease with lethal cases was registered in camels in Kazakhstan in 2018. The disease was characterised by dry, harsh hacking cough and nasal discharge with froathy and bloody liquid. Camels died in a state of hyperexcitement and asphyxia. Investigation of the case has identified the pathogenic form of Paeniclostridium sordellii as the main cause of mortality in camels. The purpose of the study is to identify the cause of mortality among camels, bacteriological and molecular genetic characteristics of the pathogen. The methodological basis of the work is collection of biological samples from camels, isolating the pathogen on media, extracting nucleic acids, obtaining libraries for next generation sequencing, bioinformatic analysis of the obtained data, pathogenicity test on mice. This article describes the cultural, genetic and phenotypic characteristics of the isolated Shetpe PS18-01 strain. It was shown that the pathogenesis was mainly characterised by respiratory signs, in contrast to the most common gastrointestinal ones.","PeriodicalId":9377,"journal":{"name":"Bulletin of the Karaganda University. “Biology, medicine, geography Series”","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82386600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-30DOI: 10.31489/2022bmg4/189-194
Sabiyeva Аssel Sabiyeva, G. Atazhanova, M. Smagulov, G. Kurmantayeva, B. Ashirbekova
This article presents the results of our studies on the assessment of the antiradical activity of dry extracts of Dracocephalum nutans L. and Dracocephalum ruyschiana L. herb in relation to the diphenylpicrylhydrazyl radical (DPPH-radical, DPPH), obtained with the use of ultrasonic extraction. This antiradical activity research method is widеly usеd to model hyperproduction of reactive oxygen species (ROS) in vitro and is one of the methods that most significantly correlated with the total antioxidant activity. To obtain dry extracts, the method of ultrasonic extraction of D. nutans and D.ruyschiana aerial parts was used. The use of ultrasound has significant advantages over traditional technologies for raw matеrials processing. In particular, it provided the deeper penetration of the solvent into the material with a cellular structure, reduced the processing time, provided a higher product yield and reproducibility, reduced solvent consumption, increased the speed of the process, and allowed the extraction of thеrmolabile substances. The equipment doеs not require largе maintenance costs; less energy is consumed for processing; as a result, the processes become more environmentally friendly and economically viable.
{"title":"Antiradical activity of dry extracts of Dracocephalum nutans and Dracocephalum ruyschiana herb growing on the territory of the Central Kazakhstan","authors":"Sabiyeva Аssel Sabiyeva, G. Atazhanova, M. Smagulov, G. Kurmantayeva, B. Ashirbekova","doi":"10.31489/2022bmg4/189-194","DOIUrl":"https://doi.org/10.31489/2022bmg4/189-194","url":null,"abstract":"This article presents the results of our studies on the assessment of the antiradical activity of dry extracts of Dracocephalum nutans L. and Dracocephalum ruyschiana L. herb in relation to the diphenylpicrylhydrazyl radical (DPPH-radical, DPPH), obtained with the use of ultrasonic extraction. This antiradical activity research method is widеly usеd to model hyperproduction of reactive oxygen species (ROS) in vitro and is one of the methods that most significantly correlated with the total antioxidant activity. To obtain dry extracts, the method of ultrasonic extraction of D. nutans and D.ruyschiana aerial parts was used. The use of ultrasound has significant advantages over traditional technologies for raw matеrials processing. In particular, it provided the deeper penetration of the solvent into the material with a cellular structure, reduced the processing time, provided a higher product yield and reproducibility, reduced solvent consumption, increased the speed of the process, and allowed the extraction of thеrmolabile substances. The equipment doеs not require largе maintenance costs; less energy is consumed for processing; as a result, the processes become more environmentally friendly and economically viable.","PeriodicalId":9377,"journal":{"name":"Bulletin of the Karaganda University. “Biology, medicine, geography Series”","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90058834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-30DOI: 10.31489/2022bmg4/148-163
O. Cherednichenko, N. Mit, I. Magda, A. L. Pilyugina, B. Bekmanov, N. S. Mamilov, M. Chirikova, Natalya Lvovna Nigai
To assess the degree of contamination and to preserve the natural state of the Altyn-Emel State National Park (Kazakhstan), the monitoring studies were conducted. The total amount of persistent organic pollutants and heavy metals in water and soil samples was determined. Radiodosimetric research, ecological and genetic monitoring of indicator groups of animals were carried out. Chemical analysis revealed the presence of some pollutants exceeding the maximum permissible concentrations in water and soil samples. No area radioactive contamination was detected within the boundaries of the park zone, and the EDR values in general do not exceed the normative values. The exception is the local area of the “Suluchekinskoye” uranium deposit, for which EDR values above the normative values were determined. A comparative analysis of indicator animals showed that their cytogenetic homeostasis, morpho-functional indicators, habitus, and ecological characteristics are matched to the normal indexes of animals living in Kazakhstan. Thus, monitoring studies show that the ecological situation in the “Altyn-Emel” National Park is satisfactory, with some tense circumstances that require constant monitoring.
{"title":"Ecological characteristics of the area and evaluation of bio indicator species condition in Altyn-Emel State National Natural Park","authors":"O. Cherednichenko, N. Mit, I. Magda, A. L. Pilyugina, B. Bekmanov, N. S. Mamilov, M. Chirikova, Natalya Lvovna Nigai","doi":"10.31489/2022bmg4/148-163","DOIUrl":"https://doi.org/10.31489/2022bmg4/148-163","url":null,"abstract":"To assess the degree of contamination and to preserve the natural state of the Altyn-Emel State National Park (Kazakhstan), the monitoring studies were conducted. The total amount of persistent organic pollutants and heavy metals in water and soil samples was determined. Radiodosimetric research, ecological and genetic monitoring of indicator groups of animals were carried out. Chemical analysis revealed the presence of some pollutants exceeding the maximum permissible concentrations in water and soil samples. No area radioactive contamination was detected within the boundaries of the park zone, and the EDR values in general do not exceed the normative values. The exception is the local area of the “Suluchekinskoye” uranium deposit, for which EDR values above the normative values were determined. A comparative analysis of indicator animals showed that their cytogenetic homeostasis, morpho-functional indicators, habitus, and ecological characteristics are matched to the normal indexes of animals living in Kazakhstan. Thus, monitoring studies show that the ecological situation in the “Altyn-Emel” National Park is satisfactory, with some tense circumstances that require constant monitoring.","PeriodicalId":9377,"journal":{"name":"Bulletin of the Karaganda University. “Biology, medicine, geography Series”","volume":"79 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73340342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：