Annales de biologie clinique最新文献

Over the past two decades, the brain-gut axis has received increasing scholarly attention. However, few bibliometric analyses have systematically examined this area. We aimed to visualize the current status of research in this field, summarize hotspots, and present trends through a bibliometric analysis of published publications related to the brain-intestinal axis. Publications about the brain-gut axis were selected from the Web of Science Core Database (WoSCC) database. Countries/regions, institutions, authors, journals, references, and keywords in this field were visually analyzed using VOSviewer and CiteSpace software. A total of 3320 publications were eventually retrieved from 2000 to 2021. Publications on the brain-gut axis have increased year by year, especially in the last decade. The United States was the most prolific country (943, 28.404%), while Ireland was the country with the highest average number of article citations (156.03). Before 2011, most studies focused on the brain-gut axis, with irritable bowel syndrome being the most studied disorder; whereas in the last decade, most studies focused on the gut-brain axis, especially the microbial gut-brain axis, with neurodegenerative disorders being the most studied. In terms of global trends, research on the brain-gut axis is booming. Moreover, the role and therapeutic applications of the microbial-gut-brain axis in the progression of central nervous system (CNS) diseases, especially neurodegenerative diseases, are the focus of current research and future research trends.

Breast implant-associated anaplastic large cell lymphoma (BIA-ALCL) is a rare T-cell non-Hodgkin lymphoma, representing less than 1% of breast neoplasms. Despite its low incidence, the increasing number of women with breast implants necessitates vigilance among clinicians and pathologists. BIA-ALCL presents in situ and invasive forms, with varying prognoses. The National Comprehensive Cancer Network guidelines recommend cytology, immunohistochemistry, and flow cytometry (FCM) for diagnosis. This retrospective study analyzed 16 periprosthetic fluid (PF) samples from suspected lymphoma cases between January 2018 and January 2024. Cytological and immunological analyses were performed using May Grünwald-Giemsa staining, and FCM with BD FACSCanto II and BD LYRIC cytometers. A 10 or 12-colour FCM panel including pan-T markers and CD30 was used. Of the 16 samples, 2 cases were diagnosed with BIA-ALCL. BIA-ALCL cases showed atypical CD4+ T-cells with large size and loss of CD3 and CD7. In contrast, the 14 reactive cases did not exhibit atypical cells. Key challenges included sample volume limitations, cell dilution, and distinguishing neoplastic cells from reactive ones, particularly in cases with low cell counts. FCM, combined with an extensive panel of pan-T markers and CD30, effectively differentiates anaplastic BIA-ALCL cells from reactive seroma. However, it should complement, not replace, traditional diagnostic methods. Recognizing pitfalls and correlating FCM findings with clinical and morphological data enhances diagnostic accuracy. FCM is a valuable tool for diagnosing BIA-ALCL but should be used alongside other methods. Accurate diagnosis depends on understanding sample-specific challenges and integrating FCM results with clinical context.

Strongyloidiasis (or anguillulosis) is helminthosis caused by Strongyloides stercoralis found mostly in (sub)tropical areas and sometimes in temperate settings. We report a case of strongyloidiasis hyperinfection, diagnosed on a BALF in a 46-year-old woman of senegalese origin with no recent travel history. The patient initially presented to the emergency department with worsening chronic lower back pain, asthenia and a 10-day history of general deterioration. Plasma cell leukemia was diagnosed and treated with a block of dexamethasone combined with chemotherapy. Our laboratory incidentally detected the presence of Strongyloides larvae in a BAL specimen. The patient received a prophylactic dose of Ivermectin prior to the administration of high doses of corticosteroids. Once the diagnosis of hyperinfestation syndrome had been established, curative doses of Ivermectin were administered, initially by nasogastric tube and then subcutaneously. The evolution was rapidly unfavorable, with the appearance of edema and cerebral hemorrhages which led to the patient's death 10 days later.

The laboratory of the National Institute of Public health makes HIV-1 plasma RNA quantification. That test is required to follow up HIV patient on ART (Antiretroviral therapy). The laboratory was accredited by the West African Accreditation System (SOAC) in 2022 for HIV real time PCR. Here we report the experience of the laboratory for verifiying performance of the COBAS® AmpliPrep/COBAS® TaqMan (CAP/CTM) HIV-1 test, v 2.0 kit for measuring viral load by real time PCR. According with the technical guides of the French Accreditation Committee (COFRAC) and the recommendations of the French Society of Clinical Biology (SFBC), different steps were followed. These are the risk assessment, the study of reliability, intermediate precision, accuracy and precision of the method, estimation of measurement uncertainty and comparison of methods. Coefficient of variation for repeatability, reproducibility and trueness were within target of 5 %. The results from the CAP/CTM were reproductible and identical to those of the COBAS 6800. The COBAS® AmpliPrep/COBAS® TaqMan HIV-1 Test, v 2.0 method is suitable for routine laboratory use and has analytical performance for its intended utility, which mean the assessment of disease progression and monitoring of antiretroviral therapy for HIV-1 patients.

Despite being primarily affecting males, hemophilia, a X-linked bleeding disorder, can also concern women and girls (WG) who are hemophilia carriers (HC), as they can display FVIII or FIX levels < 40% and, or, bleeding manifestations even with normal levels of FVIII or IX. We sought to investigate the prevalence of hemophilia in women and girl relatives of persons with hemophilia in Burkina Faso. A descriptive cross-sectional survey was conducted from September 2021 to September 2023. Female relatives of male persons with hemophilia (PwH) currently followed-up at hospitals were included after providing a written consent and completing the ISTH bleeding assessment questionnaire. Blood samples were collected for hemostasis (Prothrombin time, activated partial thromboplastin time, Factor VIII or IX level) and other (Complete Blood Count, C-reactive protein; ABO/Rh blood typing) tests. Overall, 94 WG relatives of PwH participated. Their mean age being 34.2 ± 11.4 years. Seven out of 92 tested (7.6%) exhibited FVIII or FIX levels between 6% and 39% and then had been identified as WGH. Common bleeding symptoms were bleeding from the oral cavity and menorrhagia. We highlighted the high value of FVIII/IX testing for female relatives to PwH.

A 65-year-old man with a history of sequelae from strokes and aspiration pneumonia was hospitalized for a new episode of pneumococcal pneumonia. In the emergency department, laboratory tests revealed severe hypernatremia associated with multiple other biological abnormalities, followed by a sudden normalization of serum sodium levels.