Annales de biologie clinique最新文献

Strongyloidiasis (or anguillulosis) is helminthosis caused by Strongyloides stercoralis found mostly in (sub)tropical areas and sometimes in temperate settings. We report a case of strongyloidiasis hyperinfection, diagnosed on a BALF in a 46-year-old woman of senegalese origin with no recent travel history. The patient initially presented to the emergency department with worsening chronic lower back pain, asthenia and a 10-day history of general deterioration. Plasma cell leukemia was diagnosed and treated with a block of dexamethasone combined with chemotherapy. Our laboratory incidentally detected the presence of Strongyloides larvae in a BAL specimen. The patient received a prophylactic dose of Ivermectin prior to the administration of high doses of corticosteroids. Once the diagnosis of hyperinfestation syndrome had been established, curative doses of Ivermectin were administered, initially by nasogastric tube and then subcutaneously. The evolution was rapidly unfavorable, with the appearance of edema and cerebral hemorrhages which led to the patient's death 10 days later.

The laboratory of the National Institute of Public health makes HIV-1 plasma RNA quantification. That test is required to follow up HIV patient on ART (Antiretroviral therapy). The laboratory was accredited by the West African Accreditation System (SOAC) in 2022 for HIV real time PCR. Here we report the experience of the laboratory for verifiying performance of the COBAS® AmpliPrep/COBAS® TaqMan (CAP/CTM) HIV-1 test, v 2.0 kit for measuring viral load by real time PCR. According with the technical guides of the French Accreditation Committee (COFRAC) and the recommendations of the French Society of Clinical Biology (SFBC), different steps were followed. These are the risk assessment, the study of reliability, intermediate precision, accuracy and precision of the method, estimation of measurement uncertainty and comparison of methods. Coefficient of variation for repeatability, reproducibility and trueness were within target of 5 %. The results from the CAP/CTM were reproductible and identical to those of the COBAS 6800. The COBAS® AmpliPrep/COBAS® TaqMan HIV-1 Test, v 2.0 method is suitable for routine laboratory use and has analytical performance for its intended utility, which mean the assessment of disease progression and monitoring of antiretroviral therapy for HIV-1 patients.

Despite being primarily affecting males, hemophilia, a X-linked bleeding disorder, can also concern women and girls (WG) who are hemophilia carriers (HC), as they can display FVIII or FIX levels < 40% and, or, bleeding manifestations even with normal levels of FVIII or IX. We sought to investigate the prevalence of hemophilia in women and girl relatives of persons with hemophilia in Burkina Faso. A descriptive cross-sectional survey was conducted from September 2021 to September 2023. Female relatives of male persons with hemophilia (PwH) currently followed-up at hospitals were included after providing a written consent and completing the ISTH bleeding assessment questionnaire. Blood samples were collected for hemostasis (Prothrombin time, activated partial thromboplastin time, Factor VIII or IX level) and other (Complete Blood Count, C-reactive protein; ABO/Rh blood typing) tests. Overall, 94 WG relatives of PwH participated. Their mean age being 34.2 ± 11.4 years. Seven out of 92 tested (7.6%) exhibited FVIII or FIX levels between 6% and 39% and then had been identified as WGH. Common bleeding symptoms were bleeding from the oral cavity and menorrhagia. We highlighted the high value of FVIII/IX testing for female relatives to PwH.

A 65-year-old man with a history of sequelae from strokes and aspiration pneumonia was hospitalized for a new episode of pneumococcal pneumonia. In the emergency department, laboratory tests revealed severe hypernatremia associated with multiple other biological abnormalities, followed by a sudden normalization of serum sodium levels.

The diagnosis of B- and T-cell lymphomas relies on a multidisciplinary approach that combines morphological, immunological (via flow cytometry - FCM), and genetic analyses. The integration of cytological evaluation from tissue biopsy imprints with FCM enables rapid diagnostic orientation, which is valuable for guiding further complementary investigations. This atlas illustrates the main types of B- and T-cell lymphomas using cytology images, FCM data, and histological analyses derived from lymph node and splenic samples. The FCM profiles were established using routinely employed antibody panels. While results may show slight variations depending on the cytometer settings and fluorochromes used, they remain generally comparable across different instruments. An orientation panel consisting of 16 antibodies is used for the initial classification of lymphomas, with specific markers allowing for subsequent assessment of antigen expression according to cell type and pathological context. The combined study of cytological and histological features provides an integrated perspective of lymphoid pathology.

Malaria is a major public health issue in Niger. This study aims to provide information on the therapeutic efficacy of antimalarials, specifically focusing on artemether-lumefantrin (AL) in children aged 5 to 15 years in Aderbissinat, Aguié, and Baban Tabki. To evaluate the therapeutic efficacy of AL in children in the specified regions. The study was conducted from August 26 to October 25, 2022, using the standard WHO/2015 28-day follow-up method. Biological confirmation was done using rapid diagnostic tests (RDT) and microscopy, while PCR was used to distinguish between reinfections and recrudescences. Out of 352 suspected malaria patients, 263 were included in the study. There were 5 lost to follow-up, 1 voluntary withdrawal, and 3 protocol violations. The malaria prevalence was 92%, with an average parasite density of 42,606 P/μL and a sex-ratio of 1.02. The overall adequate clinical and parasitological response (ACPR) after PCR correction was 97.25%, with 100% in Aderbissinat and Aguié, and 90% in Baban Tabki. No major adverse events were recorded. The results support the use of AL for the treatment of uncomplicated malaria as per the National Malaria Control Program (NMCP).