Pub Date : 2011-09-21DOI: 10.4314/CAJEB.V7I1.69787
J. Ngoune, Cn Tekouo Dzesse, G. Dongmo, P. Tane
La reaction dans le toluene du zinc formate dihydrate, Zn(HCOO) 2 .2H 2 O avec le 2-isopropylimidazole a temperature ambiante a conduit a l’obtention du nouveau complexe bisformatobis(2-isopropylimidazole)zinc(II) de formule [Zn(N 2 H 10 C 6 ) 2 (OOCH) 2 ]. Ce materiau a ete caracterise par diverses techniques (analyse elementaire, spectroscopies IR et RMN) et l’analyse thermogravimetrique (ATG). Les analyses spectroscopiques (IR, 1 H-RMN, 13 C- RMN) ont montre que ce materiau aurait une structure exhibant le site actif observe dans certaines metalloproteines telles que les carboxypeptidase A ou B. L’analyse thermogravimetrique montre que ce materiau est stable jusqu’a 100°C. The reaction of zinc formate with 2-isopropylimidazole at room temperature using toluene as solvent has produced the new complex bisformatobis(2-isopropylimidazole)zinc(II) or [Zn(N 2 H 10 C 6 ) 2 (OOCH) 2 ]. This compound has been characterized using different analytical techniques (elemental analysis, IR and NMR spectroscopies). The spectroscopic analysis (IR, 1 H-NMR, 13 C-NMR) showed that this material would have a structure exhibiting the active site observed in certain metalloproteins such as carboxypeptidase A or B. The thermal analysis (TGA) showed that this material is stable up to 100°.
{"title":"Composé modèle des métalloprotéines de zinc: synthèse et caractérisation du complexe bisformatobis(2-isopropylimidazole)zinc(II)","authors":"J. Ngoune, Cn Tekouo Dzesse, G. Dongmo, P. Tane","doi":"10.4314/CAJEB.V7I1.69787","DOIUrl":"https://doi.org/10.4314/CAJEB.V7I1.69787","url":null,"abstract":"La reaction dans le toluene du zinc formate dihydrate, Zn(HCOO) 2 .2H 2 O avec le 2-isopropylimidazole a temperature ambiante a conduit a l’obtention du nouveau complexe bisformatobis(2-isopropylimidazole)zinc(II) de formule [Zn(N 2 H 10 C 6 ) 2 (OOCH) 2 ]. Ce materiau a ete caracterise par diverses techniques (analyse elementaire, spectroscopies IR et RMN) et l’analyse thermogravimetrique (ATG). Les analyses spectroscopiques (IR, 1 H-RMN, 13 C- RMN) ont montre que ce materiau aurait une structure exhibant le site actif observe dans certaines metalloproteines telles que les carboxypeptidase A ou B. L’analyse thermogravimetrique montre que ce materiau est stable jusqu’a 100°C. The reaction of zinc formate with 2-isopropylimidazole at room temperature using toluene as solvent has produced the new complex bisformatobis(2-isopropylimidazole)zinc(II) or [Zn(N 2 H 10 C 6 ) 2 (OOCH) 2 ]. This compound has been characterized using different analytical techniques (elemental analysis, IR and NMR spectroscopies). The spectroscopic analysis (IR, 1 H-NMR, 13 C-NMR) showed that this material would have a structure exhibiting the active site observed in certain metalloproteins such as carboxypeptidase A or B. The thermal analysis (TGA) showed that this material is stable up to 100°.","PeriodicalId":9401,"journal":{"name":"Cameroon Journal of Experimental Biology","volume":"48 1","pages":"16-21"},"PeriodicalIF":0.0,"publicationDate":"2011-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88717711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-09-21DOI: 10.4314/CAJEB.V7I1.69786
O. Komolafe, G. Arawomo
The food and diet of two economically important freshwater fish species, Parachanna obscura and Clarias gariepinus were examined in Osinmo reservoir. Gill-net, cast-net and traps were the fishing gears used to collect fish specimens. Morphometric parameters of each fish were taken. Stomach fullness was noted and each stomach preserved in 4% formalin. Frequency of occurrence and Numerical methods was used to analyse the stomach contents of each fish. The study observed by frequency of occurrence that insects, fish, and algae, constituted 73% of the stomach contents of P. obscura throughout the sampling period. Similarly the stomach contents of C. gariepinus were dominated to the tune of 79% by detritus, mud, fish, algae, insects and diatoms. Slight variation in food items was observed during the dry and rainy seasons. The percentage of non- empty stomachs in dry and rainy seasons increased from 80% to 89% in P. obscura and from 74.6% to 83.4% in C. gariepinus . The two species of fish fed on many related food items and Schoener overlap index values of 0.05 and 0.02 indicated no diet overlap and competition in dry and rainy seasons.
{"title":"Food and diet relationships of Parachanna obscura (Gunther) and Clarias gariepinus (Burchell) in a newly impounded Osinmo reservoir, Ejigbo, Nigeria.","authors":"O. Komolafe, G. Arawomo","doi":"10.4314/CAJEB.V7I1.69786","DOIUrl":"https://doi.org/10.4314/CAJEB.V7I1.69786","url":null,"abstract":"The food and diet of two economically important freshwater fish species, Parachanna obscura and Clarias gariepinus were examined in Osinmo reservoir. Gill-net, cast-net and traps were the fishing gears used to collect fish specimens. Morphometric parameters of each fish were taken. Stomach fullness was noted and each stomach preserved in 4% formalin. Frequency of occurrence and Numerical methods was used to analyse the stomach contents of each fish. The study observed by frequency of occurrence that insects, fish, and algae, constituted 73% of the stomach contents of P. obscura throughout the sampling period. Similarly the stomach contents of C. gariepinus were dominated to the tune of 79% by detritus, mud, fish, algae, insects and diatoms. Slight variation in food items was observed during the dry and rainy seasons. The percentage of non- empty stomachs in dry and rainy seasons increased from 80% to 89% in P. obscura and from 74.6% to 83.4% in C. gariepinus . The two species of fish fed on many related food items and Schoener overlap index values of 0.05 and 0.02 indicated no diet overlap and competition in dry and rainy seasons.","PeriodicalId":9401,"journal":{"name":"Cameroon Journal of Experimental Biology","volume":"53 1","pages":"9-15"},"PeriodicalIF":0.0,"publicationDate":"2011-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85331205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-09-21DOI: 10.4314/CAJEB.V7I1.69790
A. N’Diaye, M. Sid, Ao Kankou, K. Namr
Dans le but d’evaluer les teneurs en coliformes fecaux contenus dans les effluents de la Station de Traitement des Eaux Polluees, l’Analyse en Composantes Principales de ces teneurs couplees avec les parametres physiques de l’eau (Temperature, pH, conductivite electrique et turbidite). L’analyse bacteriologique des echantillons preleves hebdomadairement au cours des mois de fevrier, de mars et d’avril 2009 montre que les effluents de la STEP sont caracterises par des teneurs en coliformes fecaux oscillant entre 3,9.10 4 UFC/100 mL et 9.10 4 /100 mL avec une moyenne de 6,5.10 4 UFC/100 mL. Ces valeurs sont largement au dessus de la norme preconisee par l’OMS pour les eaux d’irrigation et qui est de l’ordre de 10 3 UFC/100mL. Les teneurs en coliformes fecaux presentent une correlation positive et fortement significative avec la turbidite, une correlation positive faiblement significative avec la temperature et des correlations negatives et faiblement significatives avec le pH et la conductivite electrique. Parmi les parametres physiques etudies seule la turbidite s’avere etre un parametre probant dans le traitement des eaux usees afin de diminuer la charge en coliformes fecaux. Un traitement physique est necessaire surtout la decantation suivie d’une filtration. In order to assess the fecal coliform contents in the effluent of the wastewater treatment plan of the Principal Component Analysis of these contents coupled with the physicochemical parameters of water (temperature, pH, electrical conductivity and turbidity) was carried out. The bacteriological analysis of samples taken weekly during the months of February, March and April 2009 shows that the effluent were characterized by fecal coliforms contents ranging from 3,9.10 4 CFU/ 100 mL and 9,10 4 CFU/ 100 mL with an average of 6,5.10 4 / 100 mL. These values were far above the recommended norm by WHO for irrigation water which is 10 3 /100mL. Fecal coliforms have a positive and highly significant correlation with turbidity, a poorly significant positive correlation with temperature. Also, poorly significant and negative correlations were observed pH and conductivity were observed. Among the physicochemical parameters studied only the turbidity appeared as the parameter to be monitored during the treatment of wastewater to reduce the load of fecal coliforms. A physical treatment is necessary especially decantation followed by filtration.
为了评估污水处理厂废水中粪便大肠菌群的含量,对这些含量的主成分分析与水的物理参数(温度、pH值、电导率和浊度)相结合。bacteriologique preleves样品的分析,每周在2009年2月、3月和4月份显示出水STEP》由大肠菌群含量和数据caracterises 3,9.10 cfu / 100mL和4至4 / 100毫升,平均为9.10 6,5.10 4 cfu / 100mL。这些价值远远高于标准政策的灌溉用水和卫生组织为大约是3 - 10 cfu / 100mL。粪便大肠菌群含量与浊度呈正且显著相关,与温度呈正且不显著相关,与pH值和电导率呈正且不显著相关。在研究的物理参数中,只有浊度被证明是废水处理减少粪便大肠菌群负荷的有效参数。物理处理是必要的,特别是滗析和过滤。为了评估废水处理计划中废水中的粪便大肠菌群成分,对这些成分进行了主要成分分析,并结合水的物理化学参数(温度、pH值、电导率和浊度)。bacteriological样品取样分析》周刊during The以后of 2009年2月计划,March and April shows that The出水were characterized by fecal coliforms高兴ranging from 3,9.10 4 CFU / 100mL和9、10 CFU / 100mL with an average of 6,5.10 4 / 100mL以上。这些价值观were far The推荐的orm by WHO灌溉water for which is - 3 / 10毫升之间。= =地理= =根据美国人口普查,这个县的面积为,其中土地面积为。此外,观察到pH值和电导率之间的差显著性和负相关性。在研究的物理化学参数中,只有浑浊度作为废水处理过程中要监测的参数,以减少粪便大肠菌群的负荷。= =地理= =根据美国人口普查,这个县的总面积为,其中土地和(1.641平方公里)水。
{"title":"Evaluation des teneurs en coliformes fécaux par couplage avec des paramètres physicochimiques en ACP : cas des effluents de la STEP du périmètre maraicher de Sebkha, Nouakchott","authors":"A. N’Diaye, M. Sid, Ao Kankou, K. Namr","doi":"10.4314/CAJEB.V7I1.69790","DOIUrl":"https://doi.org/10.4314/CAJEB.V7I1.69790","url":null,"abstract":"Dans le but d’evaluer les teneurs en coliformes fecaux contenus dans les effluents de la Station de Traitement des Eaux Polluees, l’Analyse en Composantes Principales de ces teneurs couplees avec les parametres physiques de l’eau (Temperature, pH, conductivite electrique et turbidite). L’analyse bacteriologique des echantillons preleves hebdomadairement au cours des mois de fevrier, de mars et d’avril 2009 montre que les effluents de la STEP sont caracterises par des teneurs en coliformes fecaux oscillant entre 3,9.10 4 UFC/100 mL et 9.10 4 /100 mL avec une moyenne de 6,5.10 4 UFC/100 mL. Ces valeurs sont largement au dessus de la norme preconisee par l’OMS pour les eaux d’irrigation et qui est de l’ordre de 10 3 UFC/100mL. Les teneurs en coliformes fecaux presentent une correlation positive et fortement significative avec la turbidite, une correlation positive faiblement significative avec la temperature et des correlations negatives et faiblement significatives avec le pH et la conductivite electrique. Parmi les parametres physiques etudies seule la turbidite s’avere etre un parametre probant dans le traitement des eaux usees afin de diminuer la charge en coliformes fecaux. Un traitement physique est necessaire surtout la decantation suivie d’une filtration. In order to assess the fecal coliform contents in the effluent of the wastewater treatment plan of the Principal Component Analysis of these contents coupled with the physicochemical parameters of water (temperature, pH, electrical conductivity and turbidity) was carried out. The bacteriological analysis of samples taken weekly during the months of February, March and April 2009 shows that the effluent were characterized by fecal coliforms contents ranging from 3,9.10 4 CFU/ 100 mL and 9,10 4 CFU/ 100 mL with an average of 6,5.10 4 / 100 mL. These values were far above the recommended norm by WHO for irrigation water which is 10 3 /100mL. Fecal coliforms have a positive and highly significant correlation with turbidity, a poorly significant positive correlation with temperature. Also, poorly significant and negative correlations were observed pH and conductivity were observed. Among the physicochemical parameters studied only the turbidity appeared as the parameter to be monitored during the treatment of wastewater to reduce the load of fecal coliforms. A physical treatment is necessary especially decantation followed by filtration.","PeriodicalId":9401,"journal":{"name":"Cameroon Journal of Experimental Biology","volume":"6 1","pages":"35-40"},"PeriodicalIF":0.0,"publicationDate":"2011-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72739408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-08-02DOI: 10.4314/CAJEB.V6I2.68529
A. N’Diaye, M. Kankou, B. Lô, K. Namr
Ce travail a pour objectif d’elucider la typologie physicochimique des eaux usees utilisees en irrigation dans le perimetre maraicher de Sebkha. Les prelevements ont ete effectues hebdomadairement au cours des mois de mars et d'avril 2009. Les parametres physicochimiques et chimiques etudies sont: la temperature, le pH, la conductivite electrique, les matieres en suspension, les chlorures, les bicarbonates, le calcium, le magnesium, le sodium, le potassium, les nitrates, les nitrites, l’ammonium, les orthophosphates, le fer et le chrome. Les resultats des analyses presentes dans ce travail ont montre que ces eaux usees ont des temperatures pouvant atteindre 26,3 °C. Le pH oscillant entre 6,91 et 7,76. La salinite de ces eaux usees a ete evaluee par la mesure de la conductivite electrique, des ions chlorures, des ions sodium et le Rapport d'Adsorption du Sodium (SAR) presentant des valeurs maximales respectives de 3,98 mS/cm, 1633 mg/L, 390 mg/L et 6,3. Cette forte salinite est apportee en grande partie par les unites industrielles connectees au niveau de reseau d'assainissement. L’azote ammoniacal est la forme d’azote la plus abondante. Les ions bicarbonates, les orthphosphates, les sulfates, le potassium, le calcium, le magnesium, le fer et le chrome a des concentrations moyennes respectives de 1537,2 mg/L, 39,15 mg/L, 53,29 mg/L, 39 mg/L, 226,69 mg/L, 94,28 mg/L, 542 μg/L et 368 μg/L. L’analyse en composante principale avec utilisation d'un Logiciel XLSTAT 2010 fait apparaitre, globalement, deux sortes d’effluents : un effluent industriel presentant une forte salinite et un effluent domestique caracterise par des teneurs elevees en azote ammoniacal. Mots cles : Typologie, eau usee, Sebkha, Nouakchott, Mauritanie. The aim of this work was to elucidate the physicochemical typology of wastewater used for irrigation in the scope of market-garden in Sebkha. The samples were taken weekly during the months of March and April 2009. Chemical and physicochemical parameters studied are: temperature, pH, electrical conductivity, suspended solids, chlorides, bicarbonates, calcium, magnesium, sodium, potassium, nitrates, nitrites, ammonium, orthophosphate, iron and chromium. The test results presented in this work showed that the wastewater temperatures could reach 26.3 ° C. The pH ranged from 6.91 and 7.76. The salinity of the wastewater was assessed by measuring electrical conductivity, chloride ions, sodium ions and the Sodium Adsorption Ratio (SAR) with respective maximum values of 3.98 mS / cm, 1633 mg / L, 390 mg / L and 6.3. This high salinity is provided largely by the industrial units connected to the sewage network. Ammonia nitrogen was the most abundant form of nitrogen compound found in the sewage; Bicarbonate ions, orthphosphates, sulfates, potassium, calcium, magnesium, iron and chromium concentrations showed respective average of 1537.2 mg / L, 39.15 mg / L, 53.29 mg / L, 39 mg / L, 226.69 mg / L, 94.28 mg / L and 542 µg/L and 368 µg/L. The pr
这项工作的目的是阐明Sebkha市场周边用于灌溉的废水的物理化学类型。在2009年3月和4月期间,每周都进行取样。研究的物理化学和化学参数有:温度、pH值、电导率、悬浮物、氯化物、碳酸氢盐、钙、镁、钠、钾、硝酸盐、亚硝酸盐、铵、正磷酸盐、铁和铬。这项工作的分析结果表明,这些废水的温度高达26.3°C。pH值在6.91到7.76之间。通过测量电导率、氯离子、钠离子和钠吸附比(SAR)来评价废水的盐度,其最大值分别为3.98 mS/cm、1633 mg/L、390 mg/L和6.3。这种高盐度主要是由连接到污水网络的工业单元提供的。氨氮是氮最丰富的形式。碳酸氢盐、正磷酸盐、硫酸盐、钾、钙、镁、铁和铬离子的平均浓度分别为1536.2 mg/L、39.15 mg/L、53.29 mg/L、39 mg/L、226.69 mg/L、94.28 mg/L、542 μg/L和368 μg/L。使用XLSTAT 2010软件进行主成分分析,可以发现两种废水:一种是高盐度的工业废水,另一种是氨氮含量高的生活废水。关键词:类型学,乌西,塞布卡,努瓦克肖特,毛里塔尼亚。这项工作的目的是阐明Sebkha市场花园范围内用于灌溉的废水的物理化学类型。从样品were一首周刊during The of March and April 2009。研究的化学和物理化学参数有:温度、pH值、电导率、悬浮固体、氯化物、碳酸氢盐、钙、镁、钠、钾、硝酸盐、亚硝酸盐、铵、正磷酸盐、铁和铬。本研究提出的测试结果表明,废水温度可达26.3°c, pH值范围为6.91 ~ 7.76。通过测量电导率、氯离子、钠离子和钠吸附比(SAR)来评估废水的盐度,其最大值分别为3.98 mS / cm、1633 mg / L、390 mg / L和6.3。= =地理= =根据美国人口普查局的数据,该镇总面积为,其中土地和(1.1%)水。氨氮是在污水中发现的最丰富的氮化合物形式;碳酸氢盐离子、正磷酸盐、硫酸盐、钾、钙、镁、铁和铬的平均浓度分别为1537.2 mg /L、39.15 mg /L、53.29 mg /L、39 mg /L、226.69 mg /L、94.28 mg /L、542µg/L和368µg/L。使用XLSTAT 2010软件进行的主要成分分析显示,全球有两种废水:一种是高盐度的工业废水,另一种是以氨氮含量高为特征的家庭废水。关键词:排类学,毛里塔尼亚,努瓦克肖特,塞布卡
{"title":"Typologie physicochimique des eaux usées dans le périmètre maraîcher irrigué de Sebkha, Nouakchoot.","authors":"A. N’Diaye, M. Kankou, B. Lô, K. Namr","doi":"10.4314/CAJEB.V6I2.68529","DOIUrl":"https://doi.org/10.4314/CAJEB.V6I2.68529","url":null,"abstract":"Ce travail a pour objectif d’elucider la typologie physicochimique des eaux usees utilisees en irrigation dans le perimetre maraicher de Sebkha. Les prelevements ont ete effectues hebdomadairement au cours des mois de mars et d'avril 2009. Les parametres physicochimiques et chimiques etudies sont: la temperature, le pH, la conductivite electrique, les matieres en suspension, les chlorures, les bicarbonates, le calcium, le magnesium, le sodium, le potassium, les nitrates, les nitrites, l’ammonium, les orthophosphates, le fer et le chrome. Les resultats des analyses presentes dans ce travail ont montre que ces eaux usees ont des temperatures pouvant atteindre 26,3 °C. Le pH oscillant entre 6,91 et 7,76. La salinite de ces eaux usees a ete evaluee par la mesure de la conductivite electrique, des ions chlorures, des ions sodium et le Rapport d'Adsorption du Sodium (SAR) presentant des valeurs maximales respectives de 3,98 mS/cm, 1633 mg/L, 390 mg/L et 6,3. Cette forte salinite est apportee en grande partie par les unites industrielles connectees au niveau de reseau d'assainissement. L’azote ammoniacal est la forme d’azote la plus abondante. Les ions bicarbonates, les orthphosphates, les sulfates, le potassium, le calcium, le magnesium, le fer et le chrome a des concentrations moyennes respectives de 1537,2 mg/L, 39,15 mg/L, 53,29 mg/L, 39 mg/L, 226,69 mg/L, 94,28 mg/L, 542 μg/L et 368 μg/L. L’analyse en composante principale avec utilisation d'un Logiciel XLSTAT 2010 fait apparaitre, globalement, deux sortes d’effluents : un effluent industriel presentant une forte salinite et un effluent domestique caracterise par des teneurs elevees en azote ammoniacal. Mots cles : Typologie, eau usee, Sebkha, Nouakchott, Mauritanie. The aim of this work was to elucidate the physicochemical typology of wastewater used for irrigation in the scope of market-garden in Sebkha. The samples were taken weekly during the months of March and April 2009. Chemical and physicochemical parameters studied are: temperature, pH, electrical conductivity, suspended solids, chlorides, bicarbonates, calcium, magnesium, sodium, potassium, nitrates, nitrites, ammonium, orthophosphate, iron and chromium. The test results presented in this work showed that the wastewater temperatures could reach 26.3 ° C. The pH ranged from 6.91 and 7.76. The salinity of the wastewater was assessed by measuring electrical conductivity, chloride ions, sodium ions and the Sodium Adsorption Ratio (SAR) with respective maximum values of 3.98 mS / cm, 1633 mg / L, 390 mg / L and 6.3. This high salinity is provided largely by the industrial units connected to the sewage network. Ammonia nitrogen was the most abundant form of nitrogen compound found in the sewage; Bicarbonate ions, orthphosphates, sulfates, potassium, calcium, magnesium, iron and chromium concentrations showed respective average of 1537.2 mg / L, 39.15 mg / L, 53.29 mg / L, 39 mg / L, 226.69 mg / L, 94.28 mg / L and 542 µg/L and 368 µg/L. The pr","PeriodicalId":9401,"journal":{"name":"Cameroon Journal of Experimental Biology","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2011-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85084746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-08-02DOI: 10.4314/CAJEB.V6I2.68526
M. Yakubu, M. Akanji, M. O. Nafiu
Aqueous extract of Cochlospermum planchonii root was investigated for antidiabetic activity in alloxan-induced diabetic rats. Thirty albino rats (110.33 ± 8.69 g) were randomized into six groups (A-F) such that group A (non-diabetic) received orally 0.5 ml of distilled water, thrice daily for 10 days. Animals in groups B, C, D, E and F which were all made diabetic with alloxan (150 mg/kg b. w., i.p.) also received 0.5 ml of glibenclamide (2.5 mg/kg b. w., p.o.), 25, 50 and 100 mg/kg b. w., p.o. of the extract respectively. The blood glucose of the alloxanized rats after 48 hours which ranged from 20.50-30.20 mmol/L were significantly (P Cochlospermum planchonii root possesses anti-hyperglycemic activity similar to the reference drug. The extract of Cochlospermum planchonii root is also effective in controlling some of the disorders of metabolism associated with diabetes. Keywords: Alloxan, Cochlospermaceae, Cochlospermum planchonii, Diabetes, Metabolic disorders, Glibenclamide
{"title":"Anti-diabetic activity of aqueous extract of Cochlospermum planchonii root in alloxan-induced diabetic rats","authors":"M. Yakubu, M. Akanji, M. O. Nafiu","doi":"10.4314/CAJEB.V6I2.68526","DOIUrl":"https://doi.org/10.4314/CAJEB.V6I2.68526","url":null,"abstract":"Aqueous extract of Cochlospermum planchonii root was investigated for antidiabetic activity in alloxan-induced diabetic rats. Thirty albino rats (110.33 ± 8.69 g) were randomized into six groups (A-F) such that group A (non-diabetic) received orally 0.5 ml of distilled water, thrice daily for 10 days. Animals in groups B, C, D, E and F which were all made diabetic with alloxan (150 mg/kg b. w., i.p.) also received 0.5 ml of glibenclamide (2.5 mg/kg b. w., p.o.), 25, 50 and 100 mg/kg b. w., p.o. of the extract respectively. The blood glucose of the alloxanized rats after 48 hours which ranged from 20.50-30.20 mmol/L were significantly (P Cochlospermum planchonii root possesses anti-hyperglycemic activity similar to the reference drug. The extract of Cochlospermum planchonii root is also effective in controlling some of the disorders of metabolism associated with diabetes. Keywords: Alloxan, Cochlospermaceae, Cochlospermum planchonii, Diabetes, Metabolic disorders, Glibenclamide","PeriodicalId":9401,"journal":{"name":"Cameroon Journal of Experimental Biology","volume":"88 1","pages":"91-100"},"PeriodicalIF":0.0,"publicationDate":"2011-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88120971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-08-02DOI: 10.4314/CAJEB.V6I2.68523
N. Onguene, L.E.M. Ngonkeu, T. Kuyper
Deficiency in mycorrhizal inoculum in soils due to land use types (LUT) can be alleviated by inoculum addition. Inoculum effects may depend both on quantity and on quality of inoculum applied. A greenhouse bioassay was carried out to determine the effect of host soil mycorrhizal inoculum on mycorrhizal colonization, carbon allocation and partitioning of seedlings of two native timber species of Cameroon humid forest, raised on surface soils (0-20 cm) collected from early secondary forests, LUT derived from slash-and-burn agriculture and selective logging. Host soil mycorrhizal inoculum was collected from the root zones of con-specific tree species. Mycorrhizal inoculation effect (MIE) was estimated as percent difference of average total biomass between seedlings grown on inoculated and non-inoculated soils. Six months-old seedlings of Pterocarpus soyauxii and Lophira alata were mainly colonized by members of the Glomaceae and Gigasporaceae, respectively, as shown by molecular typing. They generally performed poorly in soils with indigenous inoculum. But addition of soil inoculum from P. soyauxii trees favored nodulation, significantly increased mycorrhizal colonization and total biomass but decreased root-to-shoot ratios, resulting in large and positive MIE, irrespective of LUT. ln contrast, host soil inoculum of L. alata did not affect fractional mycorrhizal colonization but significantly increased total biomass, resulted in high carbon allocation to roots, in low and sometimes negative MIE. Therefore, seedlings' responses to mycorrhizal inoculum depend on host soil inoculum and that could be critical for successful rejuvenation of tropical trees. Key words. Arbuscular mycorrhiza-host soil inoculum-Iand use types- Pterocarpus soyauxii-Lophira alata -Cameroon
{"title":"Growth response of Pterocarpus soyauxii and Lophira alata seedlings to host soil mycorrhizal inocula in relation to land use types","authors":"N. Onguene, L.E.M. Ngonkeu, T. Kuyper","doi":"10.4314/CAJEB.V6I2.68523","DOIUrl":"https://doi.org/10.4314/CAJEB.V6I2.68523","url":null,"abstract":"Deficiency in mycorrhizal inoculum in soils due to land use types (LUT) can be alleviated by inoculum addition. Inoculum effects may depend both on quantity and on quality of inoculum applied. A greenhouse bioassay was carried out to determine the effect of host soil mycorrhizal inoculum on mycorrhizal colonization, carbon allocation and partitioning of seedlings of two native timber species of Cameroon humid forest, raised on surface soils (0-20 cm) collected from early secondary forests, LUT derived from slash-and-burn agriculture and selective logging. Host soil mycorrhizal inoculum was collected from the root zones of con-specific tree species. Mycorrhizal inoculation effect (MIE) was estimated as percent difference of average total biomass between seedlings grown on inoculated and non-inoculated soils. Six months-old seedlings of Pterocarpus soyauxii and Lophira alata were mainly colonized by members of the Glomaceae and Gigasporaceae, respectively, as shown by molecular typing. They generally performed poorly in soils with indigenous inoculum. But addition of soil inoculum from P. soyauxii trees favored nodulation, significantly increased mycorrhizal colonization and total biomass but decreased root-to-shoot ratios, resulting in large and positive MIE, irrespective of LUT. ln contrast, host soil inoculum of L. alata did not affect fractional mycorrhizal colonization but significantly increased total biomass, resulted in high carbon allocation to roots, in low and sometimes negative MIE. Therefore, seedlings' responses to mycorrhizal inoculum depend on host soil inoculum and that could be critical for successful rejuvenation of tropical trees. Key words. Arbuscular mycorrhiza-host soil inoculum-Iand use types- Pterocarpus soyauxii-Lophira alata -Cameroon","PeriodicalId":9401,"journal":{"name":"Cameroon Journal of Experimental Biology","volume":"17 1","pages":"63-73"},"PeriodicalIF":0.0,"publicationDate":"2011-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78671605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-08-02DOI: 10.4314/CAJEB.V6I2.68525
Ehigie Ol, R. Okonji, A. Ehigie, F. Agboola
We describe the hepatopancreas arginase activity of freshwater prawn ( Macrobrachium rosenbergii). The enzyme was isolated using reactive blue 2- agarose affinity chromatography and gel filtration on Sephadex G-150. The enzyme had a specific activity of 5.70 μmol/min/mg of protein. The enzyme exhibited a maximal activity at pH 8.5 and Km of 12.5 mM. The enzyme was capable of hydrolysing L-arginine and to a lesser extent, L-arginine monohydrochlorate and L-arginine monohydrate. The optimum temperature of the enzyme was 35 0C. The molecular weight as determined by gel filtration was approximately 160,000 dalton and SDS-PAGE, was 22,000 dalton. The different amino acids (L-lysine, L-cysteine, Lvaline, L-proline, L-aspartic acid, L-glutamic acid and L-serine) and metal ions (Ni 2+ , Co 2+ , Zn 2+ , Mn 2+ and Mg 2+ ) did not show any inhibition on the enzyme activity. The enzyme was activated with Mn 2+ and different concentration of Mn 2+ had no effect on the enzyme activity. EDTA, citrate and urea showed considerable inhibition on the enzyme activity. Key words: Freshwater prawn; arginase; uricotelism; invertebrates; hepatopancreas
{"title":"Properties of Arginase from the Hepatopancreas of Giant Freshwater Prawn ( Macrobrachium rosenbergii , de Man).","authors":"Ehigie Ol, R. Okonji, A. Ehigie, F. Agboola","doi":"10.4314/CAJEB.V6I2.68525","DOIUrl":"https://doi.org/10.4314/CAJEB.V6I2.68525","url":null,"abstract":"We describe the hepatopancreas arginase activity of freshwater prawn ( Macrobrachium rosenbergii). The enzyme was isolated using reactive blue 2- agarose affinity chromatography and gel filtration on Sephadex G-150. The enzyme had a specific activity of 5.70 μmol/min/mg of protein. The enzyme exhibited a maximal activity at pH 8.5 and Km of 12.5 mM. The enzyme was capable of hydrolysing L-arginine and to a lesser extent, L-arginine monohydrochlorate and L-arginine monohydrate. The optimum temperature of the enzyme was 35 0C. The molecular weight as determined by gel filtration was approximately 160,000 dalton and SDS-PAGE, was 22,000 dalton. The different amino acids (L-lysine, L-cysteine, Lvaline, L-proline, L-aspartic acid, L-glutamic acid and L-serine) and metal ions (Ni 2+ , Co 2+ , Zn 2+ , Mn 2+ and Mg 2+ ) did not show any inhibition on the enzyme activity. The enzyme was activated with Mn 2+ and different concentration of Mn 2+ had no effect on the enzyme activity. EDTA, citrate and urea showed considerable inhibition on the enzyme activity. Key words: Freshwater prawn; arginase; uricotelism; invertebrates; hepatopancreas","PeriodicalId":9401,"journal":{"name":"Cameroon Journal of Experimental Biology","volume":"28 1","pages":"81-90"},"PeriodicalIF":0.0,"publicationDate":"2011-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86625154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-08-02DOI: 10.4314/CAJEB.V6I2.68528
L. Lienou, P. Telefo, B. Bayala, D. Yemele, M. Lemfack, C. Mouokeu, S. C. Goka, R. S. Tagne, P. Moundipa
Senecio biafrae is a medicinal plant widely used by traditional healers in the western region of Cameroon for the treatment of woman infertility . In the course of the evaluation of its effect on puberty onset and some physiological parameters of fertility, different doses of its ethanolic extract were orally administered to immature female rats for 30 days. Body, ovarian, uterus weight; uterine, ovarian proteins or cholesterol level as well as data on puberty onset were recorded. A linear increase in the growth rate of all animal was observed throughout the experimental period. From the 27 th day till the end of treatment, the body weight gain of rats treated with 8 mg/kg was significantly increased (p Overall, the present results provide evidence on the estrogenic effect of the ethanolic extract of Senecio biafrae leading to the acceleration of puberty onset in immature female rat. Key words : Fertility; Senecio biafrae ; puberty; ovaries; uterus
{"title":"Effect of ethanolic extract of Senecio biafrae on puberty onset and fertility in immature female rat.","authors":"L. Lienou, P. Telefo, B. Bayala, D. Yemele, M. Lemfack, C. Mouokeu, S. C. Goka, R. S. Tagne, P. Moundipa","doi":"10.4314/CAJEB.V6I2.68528","DOIUrl":"https://doi.org/10.4314/CAJEB.V6I2.68528","url":null,"abstract":"Senecio biafrae is a medicinal plant widely used by traditional healers in the western region of Cameroon for the treatment of woman infertility . In the course of the evaluation of its effect on puberty onset and some physiological parameters of fertility, different doses of its ethanolic extract were orally administered to immature female rats for 30 days. Body, ovarian, uterus weight; uterine, ovarian proteins or cholesterol level as well as data on puberty onset were recorded. A linear increase in the growth rate of all animal was observed throughout the experimental period. From the 27 th day till the end of treatment, the body weight gain of rats treated with 8 mg/kg was significantly increased (p Overall, the present results provide evidence on the estrogenic effect of the ethanolic extract of Senecio biafrae leading to the acceleration of puberty onset in immature female rat. Key words : Fertility; Senecio biafrae ; puberty; ovaries; uterus","PeriodicalId":9401,"journal":{"name":"Cameroon Journal of Experimental Biology","volume":"6 1","pages":"101-109"},"PeriodicalIF":0.0,"publicationDate":"2011-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78471250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-08-02DOI: 10.4314/CAJEB.V6I2.68524
P. Zerbo, B. Belem, J. Mllogo-Rasolodimby, P. V. Damme
Burkina Faso. Des solutions imperatives devraient etre trouvees en vue d’une gestion durable desdites especes. Dans cette optique, une etude sur la germination sexuee d’Ozoroa insignis Del. a ete conduite au laboratoire et en pepiniere. L’effet du pretraitement et celui substrat sur la germination des semences ont ete etudies. Le developpement morphologique precoce des plantules a ete aussi etudie. Les resultats ont revele que la germination est influencee par le type traitement et du substrat utilise. Le traitement acide et le substrat sableux offrent de meilleurs taux moyens de germination. Les fortes chaleurs (cuisson et ebouillantage) semblent nefastes aux semences. Les plantules ont presente un developpement precoce. Cette croissance rapide est un atout a la regeneration en milieu naturel. Mots-cles : Ozoroa insignis , Germination sexuee, Croissance, Burkina Faso The great medicinal plant used in Burkina Faso is a serious threat of some of them. Compelling solutions should be found for these species sustainable management. Then, Ozoroa insignis Del sexual germination were conducted in the laboratory and nursery. The effect of pretreatment and the substrate on seed germination were studied. Seedlings morphological growing was also studied. The results showed that germination is influenced by the treatment and the substrate used. The acid treatment and the sandy substrate offer better means of germination. High temperature (cooking and boiling) seem harmful to seeds. Seedlings showed a quite development taproot system. This rapid growth is an asset for regeneration in the wild. Keywords : Ozoroa insignis , Sexual germination, Growth, Burkina Faso
{"title":"Germination sexuée et croissance précoce d’Ozoroa insignis Del., une espèce médicinale du Burkina Faso","authors":"P. Zerbo, B. Belem, J. Mllogo-Rasolodimby, P. V. Damme","doi":"10.4314/CAJEB.V6I2.68524","DOIUrl":"https://doi.org/10.4314/CAJEB.V6I2.68524","url":null,"abstract":"Burkina Faso. Des solutions imperatives devraient etre trouvees en vue d’une gestion durable desdites especes. Dans cette optique, une etude sur la germination sexuee d’Ozoroa insignis Del. a ete conduite au laboratoire et en pepiniere. L’effet du pretraitement et celui substrat sur la germination des semences ont ete etudies. Le developpement morphologique precoce des plantules a ete aussi etudie. Les resultats ont revele que la germination est influencee par le type traitement et du substrat utilise. Le traitement acide et le substrat sableux offrent de meilleurs taux moyens de germination. Les fortes chaleurs (cuisson et ebouillantage) semblent nefastes aux semences. Les plantules ont presente un developpement precoce. Cette croissance rapide est un atout a la regeneration en milieu naturel. Mots-cles : Ozoroa insignis , Germination sexuee, Croissance, Burkina Faso The great medicinal plant used in Burkina Faso is a serious threat of some of them. Compelling solutions should be found for these species sustainable management. Then, Ozoroa insignis Del sexual germination were conducted in the laboratory and nursery. The effect of pretreatment and the substrate on seed germination were studied. Seedlings morphological growing was also studied. The results showed that germination is influenced by the treatment and the substrate used. The acid treatment and the sandy substrate offer better means of germination. High temperature (cooking and boiling) seem harmful to seeds. Seedlings showed a quite development taproot system. This rapid growth is an asset for regeneration in the wild. Keywords : Ozoroa insignis , Sexual germination, Growth, Burkina Faso","PeriodicalId":9401,"journal":{"name":"Cameroon Journal of Experimental Biology","volume":"402 4","pages":"74-80"},"PeriodicalIF":0.0,"publicationDate":"2011-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91449524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-07-21DOI: 10.4314/CAJEB.V6I1.56875
B. Ouattara, Ow Savadogo, O. Traoré, B. Koulibali, M. P. Sedogo, A. Traoré
L’effet des pesticides utilises en culture cotonniere sur l’activite microbienne globale des sols a ete etudie sur un sol ferrugineux tropical en zone ouest du Burkina Faso. Des echantillons de sol ont ete preleves a 30, 73, et 119 jours apres semis du cotonnier. Les analyses de respirometrie, de determination de la biomasse microbienne et de l’ammonification ont ete realisees. Les resultats indiquent que les doses de pesticides apportees sont insuffisantes pour affecter les bacteries ammonifiantes. En revanche, ces doses perturbent la vie du sol en affectant l’efficience des microorganismes a utiliser la matiere organique, en activant tantot la respiration et la biomasse microbiennes et tantot en les inhibant. Mots Cles : Pesticide – Sol – Activite Respiratoire – Biomasse Microbienne – Bacterie Ammonifiante The effect of pesticides used in cotton cultivation on soil global microbial activity was studied on a tropical ferruginous soil in the west zone of Burkina Faso. The soil samples were took at 30, 73 and 119 days after the cotton seeds. The analyses of respirometry, of microbial biomas determination and of the ammonification were realized. The results indicate that the doses of pesticides that put aren’t enough to affect ammonificate bacteriums. In return, these doses disrupt soil life by affecting microorganism’s efficiency in using organic matter, by stimulating sometimes microbial’s respiration and biomas, and sometimes inhibiting it. Key words: Pesticide – Soil – Respiratory activity – Microbial biomas – Ammonificate bacterium
{"title":"Effet des pesticides sur l'activité microbienne d'un sol ferrugineux tropical du Burkina Faso.","authors":"B. Ouattara, Ow Savadogo, O. Traoré, B. Koulibali, M. P. Sedogo, A. Traoré","doi":"10.4314/CAJEB.V6I1.56875","DOIUrl":"https://doi.org/10.4314/CAJEB.V6I1.56875","url":null,"abstract":"L’effet des pesticides utilises en culture cotonniere sur l’activite microbienne globale des sols a ete etudie sur un sol ferrugineux tropical en zone ouest du Burkina Faso. Des echantillons de sol ont ete preleves a 30, 73, et 119 jours apres semis du cotonnier. Les analyses de respirometrie, de determination de la biomasse microbienne et de l’ammonification ont ete realisees. Les resultats indiquent que les doses de pesticides apportees sont insuffisantes pour affecter les bacteries ammonifiantes. En revanche, ces doses perturbent la vie du sol en affectant l’efficience des microorganismes a utiliser la matiere organique, en activant tantot la respiration et la biomasse microbiennes et tantot en les inhibant. Mots Cles : Pesticide – Sol – Activite Respiratoire – Biomasse Microbienne – Bacterie Ammonifiante The effect of pesticides used in cotton cultivation on soil global microbial activity was studied on a tropical ferruginous soil in the west zone of Burkina Faso. The soil samples were took at 30, 73 and 119 days after the cotton seeds. The analyses of respirometry, of microbial biomas determination and of the ammonification were realized. The results indicate that the doses of pesticides that put aren’t enough to affect ammonificate bacteriums. In return, these doses disrupt soil life by affecting microorganism’s efficiency in using organic matter, by stimulating sometimes microbial’s respiration and biomas, and sometimes inhibiting it. Key words: Pesticide – Soil – Respiratory activity – Microbial biomas – Ammonificate bacterium","PeriodicalId":9401,"journal":{"name":"Cameroon Journal of Experimental Biology","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74921035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}