首页 > 最新文献

Polish journal of veterinary sciences最新文献

英文 中文
Essential oil and plant extract of oregano as agents influencing the virulence factors of Candida albicans. 牛至精油和植物提取物是影响白色念珠菌毒力因子的物质。
Pub Date : 2024-03-20 DOI: 10.24425/pjvs.2024.149330
P Váczi, M Proškovcová, E Čonková, D Marcinčáková, M Bačkorová, M Harčárová

Candida albicans, a polymorphic yeast, is a physiological component of the human and animal commensal microbiome. It is an etiological factor of candidiasis, which is treated by azole antifungals. Growing resistance to azoles is a reason to look for other alternative treatment options. The pharmacotherapeutic use of plant extracts and essential oils has become increasingly important. In our experiment, C. albicans showed susceptibility to four observed plant extracts and essential oils from peppermint ( Mentha piperita), thyme ( Thymus vulgaris), sage ( Salvia officinalis), and oregano ( Origanum vulgare). Oregano plant extract and essential oil showed the highest antifungal activity, at MIC values of 4.9 mg/mL and 0.4 mg/mL respectively. Therefore, it was subjected to further research on the influence of virulence factors - biofilm formation, extracellular phospholipase production and germ tube formation. Oregano plant extract and essential oil showed an inhibitory effect on the observed C. albicans virulence factors at relatively low concentrations. The extract inhibited the adherence of cells at MIC 12.5 mg/mL and essential oil at MIC 0.25 mg/mL. Degradation of the formed biofilm was detected at MIC 14.1 mg/mL for plant extract and at MIC 0.4 mg/mL for essential oil. Extracellular phospholipase production was most effectively inhibited by the essential oil. In particular, the number of isolates with intensive extracellular phospholipase production decreased significantly. Of the 12 isolates intensively producing extracellular phospholipase, only 1 isolate (4.5%) retained intense production. Essential oil caused up to a 100 % reduction in germ tubes formation and plant extract reduced their formation depending on the concentration as follows: 2.6% (0.8 mg/mL), 21.2 % (6.25 mg/mL), and 64.5 % (12.5 mg/mL) compared to the control.

白色念珠菌是一种多态酵母菌,是人类和动物共生微生物组的生理组成部分。它是念珠菌病的致病因素之一,可通过唑类抗真菌药物治疗。唑类抗真菌药的抗药性不断增加,因此需要寻找其他替代治疗方案。植物提取物和精油的药物治疗用途变得越来越重要。在我们的实验中,白僵菌对四种观察到的植物提取物和精油表现出敏感性,它们分别来自薄荷(Mentha piperita)、百里香(Thymeus vulgaris)、鼠尾草(Salvia officinalis)和牛至(Origanum vulgare)。牛至植物提取物和精油显示出最高的抗真菌活性,其 MIC 值分别为 4.9 毫克/毫升和 0.4 毫克/毫升。因此,我们对毒力因素--生物膜的形成、细胞外磷脂酶的产生和芽管的形成--的影响进行了进一步研究。牛至植物提取物和精油在相对较低的浓度下对观察到的白僵菌毒力因子有抑制作用。提取物抑制细胞粘附的 MIC 值为 12.5 毫克/毫升,精油抑制细胞粘附的 MIC 值为 0.25 毫克/毫升。植物提取物的 MIC 值为 14.1 毫克/毫升,精油的 MIC 值为 0.4 毫克/毫升。精油能最有效地抑制细胞外磷脂酶的产生。特别是,大量产生细胞外磷脂酶的分离物数量明显减少。在 12 个密集产生细胞外磷脂酶的分离物中,只有 1 个分离物(4.5%)仍能保持密集产生。精油可使胚芽管的形成减少 100%,而植物提取物则可减少胚芽管的形成,具体浓度如下:与对照组相比,分别减少了 2.6%(0.8 毫克/毫升)、21.2%(6.25 毫克/毫升)和 64.5%(12.5 毫克/毫升)。
{"title":"Essential oil and plant extract of oregano as agents influencing the virulence factors of Candida albicans.","authors":"P Váczi, M Proškovcová, E Čonková, D Marcinčáková, M Bačkorová, M Harčárová","doi":"10.24425/pjvs.2024.149330","DOIUrl":"10.24425/pjvs.2024.149330","url":null,"abstract":"<p><p>Candida albicans, a polymorphic yeast, is a physiological component of the human and animal commensal microbiome. It is an etiological factor of candidiasis, which is treated by azole antifungals. Growing resistance to azoles is a reason to look for other alternative treatment options. The pharmacotherapeutic use of plant extracts and essential oils has become increasingly important. In our experiment, C. albicans showed susceptibility to four observed plant extracts and essential oils from peppermint ( Mentha piperita), thyme ( Thymus vulgaris), sage ( Salvia officinalis), and oregano ( Origanum vulgare). Oregano plant extract and essential oil showed the highest antifungal activity, at MIC values of 4.9 mg/mL and 0.4 mg/mL respectively. Therefore, it was subjected to further research on the influence of virulence factors - biofilm formation, extracellular phospholipase production and germ tube formation. Oregano plant extract and essential oil showed an inhibitory effect on the observed C. albicans virulence factors at relatively low concentrations. The extract inhibited the adherence of cells at MIC 12.5 mg/mL and essential oil at MIC 0.25 mg/mL. Degradation of the formed biofilm was detected at MIC 14.1 mg/mL for plant extract and at MIC 0.4 mg/mL for essential oil. Extracellular phospholipase production was most effectively inhibited by the essential oil. In particular, the number of isolates with intensive extracellular phospholipase production decreased significantly. Of the 12 isolates intensively producing extracellular phospholipase, only 1 isolate (4.5%) retained intense production. Essential oil caused up to a 100 % reduction in germ tubes formation and plant extract reduced their formation depending on the concentration as follows: 2.6% (0.8 mg/mL), 21.2 % (6.25 mg/mL), and 64.5 % (12.5 mg/mL) compared to the control.</p>","PeriodicalId":94175,"journal":{"name":"Polish journal of veterinary sciences","volume":"27 1","pages":"25-33"},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140178392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Occurrence of Macrorhabdus ornithogaster in exotic birds. 外来鸟类中出现 Macrorhabdus ornithogaster。
Pub Date : 2024-03-20 DOI: 10.24425/pjvs.2024.149335
B Blagojević, I Davidov, A Galfi Vukomanović, D Tekić, M Došenović Marinković, V Vidović

Avian gastric yeast (Macrorhabdus ornithogaster) is a microorganism that infects aviary birds worldwide, both captive and wild. A total number of 352 birds, belonging to 18 avian species, were examined from 2019 to 2022 for M. ornithogaster, using fecal smears of live birds or cytological samples of the proventriculus taken at necropsy. These cytological samples were taken from birds that died from different causes. Some of the birds exhibited symptoms such as lethargy, regurgitation, weight loss and anorexia. Faecal samples were collected from all the birds and analysed for gastric yeast using a direct smear and Gram-staining method. The microorganism was diagnosed most frequently in budgerigars (55.5%), the African gray parrot (33.3%), and nymphs (34.3%). The prevalence of M. ornithogaster in canaries was 10%. The infection was detected in 31% of the examined birds, which shows that the occurrence of M. ornithogaster in exotic birds is common. No clinical signs were observed in the vast majority of birds that tested positive for gastric yeast.

禽胃酵母菌(Macrorhabdus ornithogaster)是一种感染世界各地圈养和野生鸟类的微生物。从2019年到2022年,共对352只鸟类(分属18个鸟类物种)进行了鸟胃酵母菌检查,采用的是活鸟的粪便涂片或尸体解剖时采集的胃窦细胞学样本。这些细胞学样本取自死于不同原因的鸟类。部分鸟类表现出嗜睡、反胃、体重减轻和厌食等症状。从所有鸟类身上采集粪便样本,采用直接涂片和革兰氏染色法分析胃酵母菌。虎皮鹦鹉(55.5%)、非洲灰鹦鹉(33.3%)和若虫(34.3%)最常感染这种微生物。金丝雀的 M. ornithogaster 感染率为 10%。在 31% 的受检鸟类中发现了这种感染,这表明鸟疫鲭鲭虫在外来鸟类中的发生率很高。绝大多数胃酵母菌检测呈阳性的鸟类均未出现临床症状。
{"title":"Occurrence of Macrorhabdus ornithogaster in exotic birds.","authors":"B Blagojević, I Davidov, A Galfi Vukomanović, D Tekić, M Došenović Marinković, V Vidović","doi":"10.24425/pjvs.2024.149335","DOIUrl":"10.24425/pjvs.2024.149335","url":null,"abstract":"<p><p>Avian gastric yeast (Macrorhabdus ornithogaster) is a microorganism that infects aviary birds worldwide, both captive and wild. A total number of 352 birds, belonging to 18 avian species, were examined from 2019 to 2022 for M. ornithogaster, using fecal smears of live birds or cytological samples of the proventriculus taken at necropsy. These cytological samples were taken from birds that died from different causes. Some of the birds exhibited symptoms such as lethargy, regurgitation, weight loss and anorexia. Faecal samples were collected from all the birds and analysed for gastric yeast using a direct smear and Gram-staining method. The microorganism was diagnosed most frequently in budgerigars (55.5%), the African gray parrot (33.3%), and nymphs (34.3%). The prevalence of M. ornithogaster in canaries was 10%. The infection was detected in 31% of the examined birds, which shows that the occurrence of M. ornithogaster in exotic birds is common. No clinical signs were observed in the vast majority of birds that tested positive for gastric yeast.</p>","PeriodicalId":94175,"journal":{"name":"Polish journal of veterinary sciences","volume":"27 1","pages":"139-142"},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140178504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Raw diets for dogs and cats: Potential health benefits and threats. 猫狗的生食:潜在的健康益处和威胁。
Pub Date : 2024-03-20 DOI: 10.24425/pjvs.2024.149344
D Główny, N Sowińska, A Cieślak, M Gogulski, K Konieczny, M Szumacher-Strabel

Raw meat-based diets for pet nutrition are becoming increasingly popular. The percentage of meat content, composition of nutrients, and amount of additives started to play an important role in the recipe of a given food. However, the use of healthier and unprocessed food must also be balanced with the animal's specific needs based on its anatomy, physiology, and behavior. There are many potential advantages and disadvantages of a biologically appropriate raw food (BARF) diet, and all of them should be considered before switching to this approach. Raw meat is considered a diet closest to nature and least processed. However, raw diets threaten pet health because of the potential for nutrient imbalances. The choice of raw meat in pets' everyday diet should be supported by the veterinarian's medical decision and preferably also with nutritionist help. Growing animals require a specific Ca:P ratio in their diet, which may be improper in raw meat. For cats, taurine levels must be carefully checked. In addition, an imbalanced raw-meat diet can be the cause of poor semen quality in males. Females are prone to inhibition of the estrus cycle, especially due to hyperthyroidism. Exogenous thyroid hormone intake is a real concern when feeding a neck/head meat with thyroid glands. There is also a possibility of bacterial or parasitic presence in raw meat. The present paper aims to summarize the current state of knowledge about the benefits and threats of eating a raw meat diet for the health concerns of companion animals.

以生肉为基础的宠物营养日粮越来越受欢迎。肉类含量的百分比、营养成分的组成以及添加剂的数量开始在特定食品的配方中发挥重要作用。然而,在使用更健康、未经加工的食物的同时,还必须根据动物的解剖、生理和行为来平衡动物的特定需求。生物适宜生食(BARF)饮食有许多潜在的优点和缺点,在改用这种方法之前应考虑到所有这些优点和缺点。生肉被认为是最接近自然、加工最少的饮食。但是,生食会威胁宠物的健康,因为有可能造成营养失衡。在宠物的日常饮食中选择生肉应该得到兽医的医学决定的支持,最好还能得到营养学家的帮助。成长中的动物需要食物中特定的 Ca:P 比例,而生肉中的这一比例可能不合适。对于猫来说,必须仔细检查牛磺酸水平。此外,不均衡的生肉饮食可能是导致雄性动物精液质量差的原因。雌性动物的发情周期容易受到抑制,尤其是由于甲状腺机能亢进。在饲喂带有甲状腺的颈肉/头肉时,外源性甲状腺激素的摄入量确实是一个令人担忧的问题。此外,生肉中还可能存在细菌或寄生虫。本文旨在总结目前有关生肉饮食对伴侣动物健康的益处和威胁的知识。
{"title":"Raw diets for dogs and cats: Potential health benefits and threats.","authors":"D Główny, N Sowińska, A Cieślak, M Gogulski, K Konieczny, M Szumacher-Strabel","doi":"10.24425/pjvs.2024.149344","DOIUrl":"10.24425/pjvs.2024.149344","url":null,"abstract":"<p><p>Raw meat-based diets for pet nutrition are becoming increasingly popular. The percentage of meat content, composition of nutrients, and amount of additives started to play an important role in the recipe of a given food. However, the use of healthier and unprocessed food must also be balanced with the animal's specific needs based on its anatomy, physiology, and behavior. There are many potential advantages and disadvantages of a biologically appropriate raw food (BARF) diet, and all of them should be considered before switching to this approach. Raw meat is considered a diet closest to nature and least processed. However, raw diets threaten pet health because of the potential for nutrient imbalances. The choice of raw meat in pets' everyday diet should be supported by the veterinarian's medical decision and preferably also with nutritionist help. Growing animals require a specific Ca:P ratio in their diet, which may be improper in raw meat. For cats, taurine levels must be carefully checked. In addition, an imbalanced raw-meat diet can be the cause of poor semen quality in males. Females are prone to inhibition of the estrus cycle, especially due to hyperthyroidism. Exogenous thyroid hormone intake is a real concern when feeding a neck/head meat with thyroid glands. There is also a possibility of bacterial or parasitic presence in raw meat. The present paper aims to summarize the current state of knowledge about the benefits and threats of eating a raw meat diet for the health concerns of companion animals.</p>","PeriodicalId":94175,"journal":{"name":"Polish journal of veterinary sciences","volume":"27 1","pages":"151-159"},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140178508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Virulence properties of Listeria monocytogenes isolated from meat and meat contact surfaces in a slaughterhouse. 从屠宰场的肉类和肉类接触表面分离出的单核细胞增生李斯特菌的毒力特性。
Pub Date : 2024-03-20 DOI: 10.24425/pjvs.2024.149329
F Tasci, M Sudagidan, O Yavuz, A Soyucok, A Aydin

Listeria monocytogenes is a ubiquitous microorganism that is isolated from a variety of sources such as soil, water, decaying vegetation, sewage, animal feeds, silage, farm environments and food-processing environments. This study aimed to determine the prevalence, serogroups, biofilm formation, virulence factor genes, and genetic relationships of L. monocytogenes strains isolated from beef meat and meat contact surfaces obtained from a slaughterhouse in Burdur, Turkey. In this study, a total of 179 beef meat and meat contact surface samples were analyzed for the presence of L. monocytogenes by polymerase chain reaction (PCR). Out of a total of 179 beef meat and meat contact surface samples, 83 (46.37%) were found to be contaminated with L. monocytogenes, with the highest incidence (53.01%) occurring in beef meat. In the present study, most of the isolated strains belonged to serogroups IIB and IVB (lineage I). The L. monocytogenes strain also contained monoA-B, prfA, plcA, plcB, mpl, hlyA, actA, gtcA, dltA, Fri, flaA, InlA, InlC, InlJ, and iap genes. Biofilm formation was not determined in the tested samples at pH 5.5 and different temperatures (4°C, 10°C, 25°C, and 37°C). However, strong biofilm formation was observed in 6.45% (2/31) of the strains at pH 7.0 after 48 h incubation at 37°C, and in 3.22% (1/31) of the strains at pH 7.0 after 48 h incubation at 4°C and 10°C. Pulsed-field gel electrophoresis (PFGE) results showed that L. monocytogenes isolates were clonally related, and cross-contamination was present. In addition, PFGE results also revealed that AscI had more distinguishing power than the ApaI restriction enzyme. These results indicate that L. monocytogenes detected from meat and meat contact surfaces in the slaughterhouse pose a potential risk to public health.

单核细胞增生李斯特菌是一种无处不在的微生物,可从土壤、水、腐烂植被、污水、动物饲料、青贮饲料、农场环境和食品加工环境等多种来源中分离出来。本研究旨在确定从土耳其布尔杜尔屠宰场的牛肉和肉类接触表面分离出的单核细胞增多症菌株的流行率、血清群、生物膜形成、毒力因子基因和遗传关系。这项研究通过聚合酶链式反应(PCR)分析了 179 份牛肉和肉类接触表面样本中是否存在单核细胞增生梭菌。在总共 179 份牛肉和肉类接触表面样本中,发现 83 份(46.37%)受到单核细胞增生梭菌污染,其中牛肉的污染率最高(53.01%)。在本研究中,大部分分离出的菌株属于 IIB 和 IVB 血清群(血统 I)。单核细胞增生症菌株还含有 monoA-B、prfA、plcA、plcB、mpl、hlyA、actA、gtcA、dltA、Fri、flaA、InlA、InlC、InlJ 和 iap 基因。在 pH 值为 5.5 和不同温度(4°C、10°C、25°C 和 37°C)条件下,未检测到测试样品中生物膜的形成。然而,在 37°C 培养 48 小时后,pH 值为 7.0 的样本中有 6.45%(2/31)的菌株观察到强烈的生物膜形成;在 4°C 和 10°C 培养 48 小时后,pH 值为 7.0 的样本中有 3.22%(1/31)的菌株观察到强烈的生物膜形成。脉冲场凝胶电泳(PFGE)结果表明,单核细胞增多性酵母菌分离物具有克隆相关性,存在交叉污染。此外,PFGE 结果还显示,AscI 比 ApaI 限制酶更有鉴别力。这些结果表明,从屠宰场的肉类和肉类接触表面检测到的单核细胞增生性酵母菌对公众健康构成潜在风险。
{"title":"Virulence properties of Listeria monocytogenes isolated from meat and meat contact surfaces in a slaughterhouse.","authors":"F Tasci, M Sudagidan, O Yavuz, A Soyucok, A Aydin","doi":"10.24425/pjvs.2024.149329","DOIUrl":"10.24425/pjvs.2024.149329","url":null,"abstract":"<p><p>Listeria monocytogenes is a ubiquitous microorganism that is isolated from a variety of sources such as soil, water, decaying vegetation, sewage, animal feeds, silage, farm environments and food-processing environments. This study aimed to determine the prevalence, serogroups, biofilm formation, virulence factor genes, and genetic relationships of L. monocytogenes strains isolated from beef meat and meat contact surfaces obtained from a slaughterhouse in Burdur, Turkey. In this study, a total of 179 beef meat and meat contact surface samples were analyzed for the presence of L. monocytogenes by polymerase chain reaction (PCR). Out of a total of 179 beef meat and meat contact surface samples, 83 (46.37%) were found to be contaminated with L. monocytogenes, with the highest incidence (53.01%) occurring in beef meat. In the present study, most of the isolated strains belonged to serogroups IIB and IVB (lineage I). The L. monocytogenes strain also contained monoA-B, prfA, plcA, plcB, mpl, hlyA, actA, gtcA, dltA, Fri, flaA, InlA, InlC, InlJ, and iap genes. Biofilm formation was not determined in the tested samples at pH 5.5 and different temperatures (4°C, 10°C, 25°C, and 37°C). However, strong biofilm formation was observed in 6.45% (2/31) of the strains at pH 7.0 after 48 h incubation at 37°C, and in 3.22% (1/31) of the strains at pH 7.0 after 48 h incubation at 4°C and 10°C. Pulsed-field gel electrophoresis (PFGE) results showed that L. monocytogenes isolates were clonally related, and cross-contamination was present. In addition, PFGE results also revealed that AscI had more distinguishing power than the ApaI restriction enzyme. These results indicate that L. monocytogenes detected from meat and meat contact surfaces in the slaughterhouse pose a potential risk to public health.</p>","PeriodicalId":94175,"journal":{"name":"Polish journal of veterinary sciences","volume":"27 1","pages":"13-23"},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140178510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of adaptive duration to salinity in drinking water on behavior, weight gain and blood biochemical parameters in growing goats. 饮用水盐度适应期对生长山羊行为、增重和血液生化指标的影响
Pub Date : 2024-03-20 DOI: 10.24425/pjvs.2024.149343
T Nguyen, N Nguyen Trong, N Chaiyabutr, S Thammacharoen

This experiment aimed to determine the effect of adaptive duration to saline water on behaviors, weight gain and blood biochemical parameters in growing goats. The experiment was arranged in a completely randomized design, which included four treatments with five animals per group. The goats were administered either fresh water (control) or seawater with a salinity of 1.5%, with varying durations of adaptation to seawater. The adaptive durations included an abrupt change (A0) from fresh water to seawater with a salinity of 1.5% or stepwise adaptation either 4 (A4) or 7 (A7) days of increasing saline concentrations. The results showed that dry matter intake in the non-adapted goats (A0 group) was lower than that of the control group or the adapted goats throughout the experiment (p<0.05). In contrast, water intake from drinking saline water was greater than that in the control group (p<0.05). Body weigh did not differ among the treatments; however, non-adapted goats exhibited a lower weight gain than the adapted goats (p<0.05). The goats in the A0 and A4 groups exhibited increased plasma levels of urea, AST, and ALT compared with the control and A7 groups. However, blood electrolyte levels remained unchanged and were within the normal range for goats. Therefore, it is concluded that the stepwise adaptation to seawater with a salinity of 1.5% for 21 days has no influence on productivity and health status of goats.

本实验旨在确定适应盐水的时间长短对生长山羊的行为、增重和血液生化指标的影响。实验采用完全随机设计,包括四个处理,每组五只。山羊分别饮用淡水(对照组)或盐度为 1.5% 的海水,对海水的适应时间长短不一。适应时间包括从淡水到盐度为 1.5% 的海水的突然变化(A0),或盐度浓度不断增加的 4 天(A4)或 7 天(A7)的逐步适应。结果显示,在整个实验过程中,未适应的山羊(A0 组)的干物质摄入量低于对照组或适应山羊(p<0.05)。
{"title":"Effects of adaptive duration to salinity in drinking water on behavior, weight gain and blood biochemical parameters in growing goats.","authors":"T Nguyen, N Nguyen Trong, N Chaiyabutr, S Thammacharoen","doi":"10.24425/pjvs.2024.149343","DOIUrl":"10.24425/pjvs.2024.149343","url":null,"abstract":"<p><p>This experiment aimed to determine the effect of adaptive duration to saline water on behaviors, weight gain and blood biochemical parameters in growing goats. The experiment was arranged in a completely randomized design, which included four treatments with five animals per group. The goats were administered either fresh water (control) or seawater with a salinity of 1.5%, with varying durations of adaptation to seawater. The adaptive durations included an abrupt change (A0) from fresh water to seawater with a salinity of 1.5% or stepwise adaptation either 4 (A4) or 7 (A7) days of increasing saline concentrations. The results showed that dry matter intake in the non-adapted goats (A0 group) was lower than that of the control group or the adapted goats throughout the experiment (p<0.05). In contrast, water intake from drinking saline water was greater than that in the control group (p<0.05). Body weigh did not differ among the treatments; however, non-adapted goats exhibited a lower weight gain than the adapted goats (p<0.05). The goats in the A0 and A4 groups exhibited increased plasma levels of urea, AST, and ALT compared with the control and A7 groups. However, blood electrolyte levels remained unchanged and were within the normal range for goats. Therefore, it is concluded that the stepwise adaptation to seawater with a salinity of 1.5% for 21 days has no influence on productivity and health status of goats.</p>","PeriodicalId":94175,"journal":{"name":"Polish journal of veterinary sciences","volume":"27 1","pages":"127-134"},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140178391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development and preclinical evaluation of equine-derived hyperimmune serum against SARS-CoV-2 infection in K-18 hACE2 transgenic (Tg) mice. 在 K-18 hACE2 转基因 (Tg) 小鼠体内开发抗 SARS-CoV-2 感染的马源性超免疫血清并进行临床前评估。
Pub Date : 2024-03-20 DOI: 10.24425/pjvs.2024.149336
E A Onen, E K Demirci

This study aimed to develop an equine-derived hyperimmune serum against SARS-CoV-2 and evaluate its efficacy as a potential immunotherapy tool for the treatment of known and potential variants of COVID-19 in preclinical trials. The novelty of this study is the whole virus and ALUM gel adjuvant formula. The horses were immunized using a whole inactivated SARS-CoV-2 antigen, and the final purified hyperimmune serum showed high plaque reduction neutralization (PRNT 50) neutralizing titers. The efficacy of the hyperimmune serum was evaluated histopathologically and biochemically in the lungs, hearts, and serum of K18 hACE2 transgenic mice (n=45), which is an accepted model organism for SARS-CoV-2 studies and was challenged with live SARS-CoV-2. Serum treatment improved the general condition, resulting in lower levels of proinflammatory cytokines in the blood plasma, as well as reduced viral RNA titers in the lungs and hearts. Additionally, it reduced oxidative stress significantly and lessened the severity of interstitial pneumonia in the lungs when compared to infected positive controls. The study concluded that equine-derived anti-SARS-CoV-2 antibodies could be used for COVID-19 prevention and treatment, especially in the early stages of the disease and in combination with antiviral drugs and vaccines. This treatment will benefit special patient populations such as immunocompromised individuals, as specific antibodies against SARS-CoV-2 can neutralize the virus before it enters host cells. The rapid and cost-effective production of the serum allows for its availability during the acute phase of the disease, making it a critical intervention in preventing the spread of the disease and saving lives in new variants where a vaccine is not yet developed.

这项研究旨在开发一种针对 SARS-CoV-2 的马源性超免疫血清,并在临床前试验中评估其作为治疗 COVID-19 的已知和潜在变种的潜在免疫疗法工具的疗效。这项研究的新颖之处在于采用了全病毒和 ALUM 凝胶佐剂配方。使用全灭活 SARS-CoV-2 抗原对马进行免疫,最终纯化的超免疫血清显示出较高的斑块缩小中和(PRNT 50)中和滴度。对 K18 hACE2 转基因小鼠(n=45)的肺部、心脏和血清进行了组织病理学和生物化学评估,该小鼠是 SARS-CoV-2 研究的公认模式生物,并接受了活 SARS-CoV-2 的挑战。血清治疗改善了小鼠的总体状况,降低了血浆中促炎细胞因子的水平,并降低了肺部和心脏中的病毒 RNA 滴度。此外,与受感染的阳性对照组相比,它还能显著降低氧化应激,减轻肺部间质性肺炎的严重程度。研究认为,马源性抗SARS-CoV-2抗体可用于COVID-19的预防和治疗,尤其是在疾病的早期阶段,并可与抗病毒药物和疫苗结合使用。由于抗 SARS-CoV-2 的特异性抗体能在病毒进入宿主细胞之前将其中和,因此这种治疗方法将使免疫力低下者等特殊患者群体受益。这种血清的生产速度快、成本低,可在疾病的急性期使用,因此在尚未研制出疫苗的新变种中,它是防止疾病传播和挽救生命的重要干预手段。
{"title":"Development and preclinical evaluation of equine-derived hyperimmune serum against SARS-CoV-2 infection in K-18 hACE2 transgenic (Tg) mice.","authors":"E A Onen, E K Demirci","doi":"10.24425/pjvs.2024.149336","DOIUrl":"10.24425/pjvs.2024.149336","url":null,"abstract":"<p><p>This study aimed to develop an equine-derived hyperimmune serum against SARS-CoV-2 and evaluate its efficacy as a potential immunotherapy tool for the treatment of known and potential variants of COVID-19 in preclinical trials. The novelty of this study is the whole virus and ALUM gel adjuvant formula. The horses were immunized using a whole inactivated SARS-CoV-2 antigen, and the final purified hyperimmune serum showed high plaque reduction neutralization (PRNT 50) neutralizing titers. The efficacy of the hyperimmune serum was evaluated histopathologically and biochemically in the lungs, hearts, and serum of K18 hACE2 transgenic mice (n=45), which is an accepted model organism for SARS-CoV-2 studies and was challenged with live SARS-CoV-2. Serum treatment improved the general condition, resulting in lower levels of proinflammatory cytokines in the blood plasma, as well as reduced viral RNA titers in the lungs and hearts. Additionally, it reduced oxidative stress significantly and lessened the severity of interstitial pneumonia in the lungs when compared to infected positive controls. The study concluded that equine-derived anti-SARS-CoV-2 antibodies could be used for COVID-19 prevention and treatment, especially in the early stages of the disease and in combination with antiviral drugs and vaccines. This treatment will benefit special patient populations such as immunocompromised individuals, as specific antibodies against SARS-CoV-2 can neutralize the virus before it enters host cells. The rapid and cost-effective production of the serum allows for its availability during the acute phase of the disease, making it a critical intervention in preventing the spread of the disease and saving lives in new variants where a vaccine is not yet developed.</p>","PeriodicalId":94175,"journal":{"name":"Polish journal of veterinary sciences","volume":"27 1","pages":"61-74"},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140178389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ohmic heating application with different electric fields on inactivation of Listeria monocytogenes in protein-enriched cow milk. 不同电场的欧姆加热应用对富含蛋白质的牛奶中单核细胞增生李斯特菌的灭活作用。
Pub Date : 2024-03-20 DOI: 10.24425/pjvs.2024.149333
R Y Ayyıldız, H A Ayyıldız

The aim of this study was to determine the effects of an ohmic heating (OH) process with different electric field intensities on Listeria monocytogenes inactivation in protein-enriched cow milk. Protein powder was added at rates of 2.5%, 5% and 7.5% in 1.5% fat content milk, and L. monocytogenes (ATCC 13932) strain was then inoculated into the samples. The OH process was carried out in a laboratory-type pilot unit created using stainless steel electrodes, a K-type thermocouple, a datalogger and power supply providing AC current at 0-250 V, 10 A. The inoculated milk samples were heated to 63°C by applying an electric field intensity of 10V/cm and 20V/cm. L. monocytogenes counts, pH, color measurement and hydroxymethylfurfurol levels were then determined. OH applied with an electric field intensity of 10 V/cm caused an average decrease of 5 logs in L. monocytogenes level in the samples containing 2.5% protein and decreased below the detection limit (<1 log) at the 9th minute (p<0.05). Similarly, application of an electric field intensity of 20 V/cm in milk containing 2.5% and 5% protein caused the L.monocytogenes level to decrease below the detection limit (<1 log) at 2 minutes 30 seconds (p<0.05). No change was observed in the L* (brightness) values of the samples but it was determined that there was a slight increase in pH, a* (redness) and b* (yellowness) values compared to the control group. It was observed that the inactivation of L. monocytogenes by OH depends on the duration of the OH process, protein concentration in the milk and the applied voltage gradient.

本研究旨在确定不同电场强度的欧姆加热(OH)工艺对富含蛋白质的牛奶中单核细胞增生李斯特菌灭活的影响。在脂肪含量为 1.5% 的牛奶中分别添加 2.5%、5% 和 7.5% 的蛋白粉,然后将单核细胞增生李斯特氏菌(ATCC 13932)菌株接种到样品中。接种后的牛奶样品通过 10V/cm 和 20V/cm 的电场强度加热至 63°C。然后测定单核细胞增多症菌落总数、pH 值、颜色测量值和羟甲基呋喃水平。在含有 2.5% 蛋白质的样品中,以 10V/cm 的电场强度施加羟基甲糠醛可使单核细胞增多症菌落总数平均减少 5 个对数,并降低到检测限以下 (
{"title":"Ohmic heating application with different electric fields on inactivation of Listeria monocytogenes in protein-enriched cow milk.","authors":"R Y Ayyıldız, H A Ayyıldız","doi":"10.24425/pjvs.2024.149333","DOIUrl":"10.24425/pjvs.2024.149333","url":null,"abstract":"<p><p>The aim of this study was to determine the effects of an ohmic heating (OH) process with different electric field intensities on Listeria monocytogenes inactivation in protein-enriched cow milk. Protein powder was added at rates of 2.5%, 5% and 7.5% in 1.5% fat content milk, and L. monocytogenes (ATCC 13932) strain was then inoculated into the samples. The OH process was carried out in a laboratory-type pilot unit created using stainless steel electrodes, a K-type thermocouple, a datalogger and power supply providing AC current at 0-250 V, 10 A. The inoculated milk samples were heated to 63°C by applying an electric field intensity of 10V/cm and 20V/cm. L. monocytogenes counts, pH, color measurement and hydroxymethylfurfurol levels were then determined. OH applied with an electric field intensity of 10 V/cm caused an average decrease of 5 logs in L. monocytogenes level in the samples containing 2.5% protein and decreased below the detection limit (<1 log) at the 9th minute (p<0.05). Similarly, application of an electric field intensity of 20 V/cm in milk containing 2.5% and 5% protein caused the L.monocytogenes level to decrease below the detection limit (<1 log) at 2 minutes 30 seconds (p<0.05). No change was observed in the L* (brightness) values of the samples but it was determined that there was a slight increase in pH, a* (redness) and b* (yellowness) values compared to the control group. It was observed that the inactivation of L. monocytogenes by OH depends on the duration of the OH process, protein concentration in the milk and the applied voltage gradient.</p>","PeriodicalId":94175,"journal":{"name":"Polish journal of veterinary sciences","volume":"27 1","pages":"53-60"},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140178505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Study of autonomic modulation by non-linear analysis of heart rate variability in different age groups and analysis of health status, disease and risk of death in dogs. 通过对不同年龄组心率变异性的非线性分析研究自律神经调节,并分析狗的健康状况、疾病和死亡风险。
Pub Date : 2023-12-12 DOI: 10.24425/pjvs.2023.148278
L Martinello, F G Romão, M F Godoy, L H A Machado, M H Tsunemi, M L G Lourenço

The symbolic analysis of heart rate variability (biomarker of cardiac autonomic homeostasis) is a nonlinear and effective tool for pattern extraction and classification in a series analysis, which implies the transformation of an original time series into symbols, represented by numbers. Autonomic heart rate control is influenced by different factors, and better indicators of heart rate variability are found in healthy young individuals than in older and sicker individuals. The aim of this study was to compare the indicators of heart rate variability among healthy dogs in different age groups and in health status using the nonlinear method of symbolic analysis to evaluate the diagnostic accuracy of this method for the risk of death in dogs. An increase in cardiac sympathetic modulation was observed in puppies and dogs at risk of death, which was evidenced by a marked increase of 0 V% (without variation - associated with sympathetic modulation) and a decrease in patterns of 2 V% (two variations - associated with parasympathetic modulation), while the opposite was observed in young adult dogs with increased parasympathetic modulation. Elderly dogs showed a gradual decrease in parasympathetic activity, which tended to worsen with loss of health. It is concluded that the variables of symbolic analysis may be useful to evaluate autonomic modulation in dogs and assist in the differentiation between health states, advanced disease and death throughout the life cycle and have been shown to be indices with high specificity, sensitivity and diagnostic accuracy to help identify dogs at risk of death.

心率变异性(心脏自主神经平衡的生物标志物)的符号分析是一种非线性的有效工具,可用于序列分析中的模式提取和分类,这意味着将原始时间序列转换为以数字表示的符号。自律性心率控制受不同因素的影响,健康年轻人的心率变异性指标优于老年人和病人。本研究的目的是使用非线性符号分析方法,比较不同年龄组和健康状况的健康狗的心率变异性指标,以评估该方法对狗死亡风险的诊断准确性。在幼犬和有死亡风险的犬中观察到心脏交感神经调节增加,表现为 0 V%(无变化 - 与交感神经调节有关)的明显增加和 2 V%(两次变化 - 与副交感神经调节有关)的模式减少,而在副交感神经调节增加的年轻成年犬中则观察到相反的情况。老年犬的副交感神经活动逐渐减弱,并随着健康状况的恶化而加剧。结论是,符号分析变量可用于评估狗的自律神经调节,并有助于区分狗在整个生命周期中的健康状态、晚期疾病和死亡,而且已证明这些指数具有较高的特异性、灵敏度和诊断准确性,有助于识别面临死亡风险的狗。
{"title":"Study of autonomic modulation by non-linear analysis of heart rate variability in different age groups and analysis of health status, disease and risk of death in dogs.","authors":"L Martinello, F G Romão, M F Godoy, L H A Machado, M H Tsunemi, M L G Lourenço","doi":"10.24425/pjvs.2023.148278","DOIUrl":"https://doi.org/10.24425/pjvs.2023.148278","url":null,"abstract":"<p><p>The symbolic analysis of heart rate variability (biomarker of cardiac autonomic homeostasis) is a nonlinear and effective tool for pattern extraction and classification in a series analysis, which implies the transformation of an original time series into symbols, represented by numbers. Autonomic heart rate control is influenced by different factors, and better indicators of heart rate variability are found in healthy young individuals than in older and sicker individuals. The aim of this study was to compare the indicators of heart rate variability among healthy dogs in different age groups and in health status using the nonlinear method of symbolic analysis to evaluate the diagnostic accuracy of this method for the risk of death in dogs. An increase in cardiac sympathetic modulation was observed in puppies and dogs at risk of death, which was evidenced by a marked increase of 0 V% (without variation - associated with sympathetic modulation) and a decrease in patterns of 2 V% (two variations - associated with parasympathetic modulation), while the opposite was observed in young adult dogs with increased parasympathetic modulation. Elderly dogs showed a gradual decrease in parasympathetic activity, which tended to worsen with loss of health. It is concluded that the variables of symbolic analysis may be useful to evaluate autonomic modulation in dogs and assist in the differentiation between health states, advanced disease and death throughout the life cycle and have been shown to be indices with high specificity, sensitivity and diagnostic accuracy to help identify dogs at risk of death.</p>","PeriodicalId":94175,"journal":{"name":"Polish journal of veterinary sciences","volume":"26 4","pages":"581-590"},"PeriodicalIF":0.0,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138804407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The effect of benign prostatic hyperplasia on total antioxidant capacity and protein peroxidation in canine prostatic fluid and spermatozoa. 良性前列腺增生对犬前列腺液和精子中总抗氧化能力和蛋白质过氧化的影响
Pub Date : 2023-12-12 DOI: 10.24425/pjvs.2023.148286
A Domosławska, S Zduńczyk, A Bielecka, M Kankofer

The aim of this study was to evaluate the antioxidative/oxidative status of spermatozoa and prostatic fluid in dogs with benign prostatic hyperplasia (BPH) by the determination of total antioxidant capacity and protein peroxidation markers. Study was conducted on 40 intact dogs of various breeds. The dogs were assigned to two groups: BPH group (n=20) and non-affected group (n=20). The second and third fractions of the ejaculate were collected separately by digital manipulation. Total antioxidant capacity (TAC) and the concentrations of SH-groups in sperm and prostatic fluid were determined spectrophotometrically, the concentrations of bityrosine and formylkynurenine were determined using spectrofluorimetric methods. The mean values of TAC in spermatozoa and prostatic fluid were significantly lower (p<0.05), whereas the mean contents of biotyrosine and formylkinurenine were significantly higher (p<0.05) in BPH dogs compared to control dogs. There was no statistically significant difference in the content of SH group between dogs with BPH and control dogs (p>0.05). In conclusion, the results indicate that BPH in dogs is associated with reduced total antioxidant capacity and increased protein oxidation in the prostatic fluid and spermatozoa, and suggest the importance of oxidative stress in the pathogenesis of this condition. The potential role of antioxidants in the prevention and therapy of canine BPH requires further studies.

本研究旨在通过测定总抗氧化能力和蛋白质过氧化标记物,评估良性前列腺增生症(BPH)犬精子和前列腺液的抗氧化/氧化状态。研究以 40 只不同品种的完整犬为对象。这些狗被分为两组:良性前列腺增生组(20 只)和未受影响组(20 只)。通过数字操作分别收集射精的第二和第三部分。采用分光光度法测定精子和前列腺液中的总抗氧化能力(TAC)和SH-基团的浓度,采用分光荧光法测定双酪氨酸和甲酰基尿氨酸的浓度。精子和前列腺液中 TAC 的平均值明显降低(P0.05)。总之,研究结果表明,犬良性前列腺增生症与前列腺液和精子中总抗氧化能力降低和蛋白质氧化增加有关,并表明氧化应激在该疾病的发病机制中具有重要作用。抗氧化剂在预防和治疗犬良性前列腺增生症中的潜在作用还需要进一步研究。
{"title":"The effect of benign prostatic hyperplasia on total antioxidant capacity and protein peroxidation in canine prostatic fluid and spermatozoa.","authors":"A Domosławska, S Zduńczyk, A Bielecka, M Kankofer","doi":"10.24425/pjvs.2023.148286","DOIUrl":"https://doi.org/10.24425/pjvs.2023.148286","url":null,"abstract":"<p><p>The aim of this study was to evaluate the antioxidative/oxidative status of spermatozoa and prostatic fluid in dogs with benign prostatic hyperplasia (BPH) by the determination of total antioxidant capacity and protein peroxidation markers. Study was conducted on 40 intact dogs of various breeds. The dogs were assigned to two groups: BPH group (n=20) and non-affected group (n=20). The second and third fractions of the ejaculate were collected separately by digital manipulation. Total antioxidant capacity (TAC) and the concentrations of SH-groups in sperm and prostatic fluid were determined spectrophotometrically, the concentrations of bityrosine and formylkynurenine were determined using spectrofluorimetric methods. The mean values of TAC in spermatozoa and prostatic fluid were significantly lower (p<0.05), whereas the mean contents of biotyrosine and formylkinurenine were significantly higher (p<0.05) in BPH dogs compared to control dogs. There was no statistically significant difference in the content of SH group between dogs with BPH and control dogs (p>0.05). In conclusion, the results indicate that BPH in dogs is associated with reduced total antioxidant capacity and increased protein oxidation in the prostatic fluid and spermatozoa, and suggest the importance of oxidative stress in the pathogenesis of this condition. The potential role of antioxidants in the prevention and therapy of canine BPH requires further studies.</p>","PeriodicalId":94175,"journal":{"name":"Polish journal of veterinary sciences","volume":"26 4","pages":"667-673"},"PeriodicalIF":0.0,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138804670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Vaginal aerobic bacteria of healthy bitches and those with fertility problems. 健康母犬和有生育问题母犬的阴道需氧菌。
Pub Date : 2023-12-12 DOI: 10.24425/pjvs.2023.148293
D Jagódka, E Kaczorek-Łukowska, R Graczyk, P Socha

The most common problems in veterinary practice in bitches are bacterial infections of the reproductive tract associated with fertility problems. Research to determine the correlation between the health status of female dogs and bacterial flora of the genital tract has been ongoing for years, but the results obtained by different authors are often contradictory, and do not always concern breeding bitches. Our study identified the most common aerobic bacteria in the genital tract of numerous breeding bitches population. A total of 275 breeding dogs in anestrous phase of the estrous cycle were included in this study. 198 were qualified to the first group with no genital tract infections and no reproductive disorders. 68 bitches were qualified to the second group with complications such as: infertility, abortion, foetus resorptions and newborn mortality. The type of bacterial isolates was almost the same in the healthy bitches and the group with fertility problems. The most common bacteria obtained from the vaginal tract of the tested dogs were Streptococcus spp., Staphylococcus spp., Mycoplasma canis and Escherichia coli. There were no significant differences in bacterial prevalence in the group with reproductive problems versus healthy dogs; however, we found a statistically significant difference between both groups when the numbers of bacterial strains were compared. The number of one-strain bitches was statistically higher in the problematic group than in the non-problematic one. Bacterial culturing of vaginal swab specimens from breeding bitches without clinical signs of genital disease is of little value. Furthermore, it should always be preceded by an examination (clinical, cytological or vaginoscopy etc.). The request or requirement to perform vaginal cultures that is made by some breeders, while common, is not diagnostic for any pathologic condition and the results of these cultures should never be used to determine if antibiotic therapy is indicated.

在兽医实践中,母犬最常见的问题是与生育问题相关的生殖道细菌感染。多年来,人们一直在研究母犬的健康状况与生殖道细菌菌群之间的相关性,但不同作者得出的结果往往相互矛盾,而且并不总是与繁殖母犬有关。我们的研究确定了众多繁殖母犬生殖道中最常见的需氧菌。本研究共纳入了 275 只处于发情周期发情期的种犬。第一组中有 198 只没有生殖道感染和生殖障碍。第二组中有 68 只母狗出现了并发症,如不孕、流产、胎儿死胎和新生儿死亡。健康母犬和有生育问题的母犬分离出的细菌类型几乎相同。从受检犬阴道中最常见的细菌是链球菌属、葡萄球菌属、犬支原体和大肠杆菌。有生殖问题的狗与健康狗在细菌感染率上没有明显差异;但在比较细菌菌株数量时,我们发现两组狗的差异在统计学上有显著性。从统计学角度看,有问题的组别中单株母犬的数量高于无问题的组别。对没有生殖器疾病临床症状的种母犬阴道拭子标本进行细菌培养的价值不大。此外,在进行细菌培养之前,必须先进行检查(临床、细胞学或阴道镜检查等)。有些饲养者要求或规定进行阴道培养,虽然这种做法很常见,但并不能诊断出任何病理状况,而且这些培养结果绝不能用来确定是否需要使用抗生素治疗。
{"title":"Vaginal aerobic bacteria of healthy bitches and those with fertility problems.","authors":"D Jagódka, E Kaczorek-Łukowska, R Graczyk, P Socha","doi":"10.24425/pjvs.2023.148293","DOIUrl":"https://doi.org/10.24425/pjvs.2023.148293","url":null,"abstract":"<p><p>The most common problems in veterinary practice in bitches are bacterial infections of the reproductive tract associated with fertility problems. Research to determine the correlation between the health status of female dogs and bacterial flora of the genital tract has been ongoing for years, but the results obtained by different authors are often contradictory, and do not always concern breeding bitches. Our study identified the most common aerobic bacteria in the genital tract of numerous breeding bitches population. A total of 275 breeding dogs in anestrous phase of the estrous cycle were included in this study. 198 were qualified to the first group with no genital tract infections and no reproductive disorders. 68 bitches were qualified to the second group with complications such as: infertility, abortion, foetus resorptions and newborn mortality. The type of bacterial isolates was almost the same in the healthy bitches and the group with fertility problems. The most common bacteria obtained from the vaginal tract of the tested dogs were Streptococcus spp., Staphylococcus spp., Mycoplasma canis and Escherichia coli. There were no significant differences in bacterial prevalence in the group with reproductive problems versus healthy dogs; however, we found a statistically significant difference between both groups when the numbers of bacterial strains were compared. The number of one-strain bitches was statistically higher in the problematic group than in the non-problematic one. Bacterial culturing of vaginal swab specimens from breeding bitches without clinical signs of genital disease is of little value. Furthermore, it should always be preceded by an examination (clinical, cytological or vaginoscopy etc.). The request or requirement to perform vaginal cultures that is made by some breeders, while common, is not diagnostic for any pathologic condition and the results of these cultures should never be used to determine if antibiotic therapy is indicated.</p>","PeriodicalId":94175,"journal":{"name":"Polish journal of veterinary sciences","volume":"26 4","pages":"733-739"},"PeriodicalIF":0.0,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138805818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Polish journal of veterinary sciences
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1