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Morphometric assessment of the choroid in dogs diagnosed with retinal atrophy (RA) with symptoms of progressive retinal atrophy, using spectral-domain optical coherence tomography (SD-OCT). 使用光谱域光学相干断层扫描(SD-OCT)对诊断为视网膜萎缩(RA)并伴有进行性视网膜萎缩症状的狗的脉络膜进行形态计量学评估。
Pub Date : 2024-06-01 DOI: 10.24425/pjvs.2024.149356
J Zwolska, I Balicki, A Balicka, B Kuduk

The aim of the study was to determine the thickness of choroidal layers in mixed breed dogs suffering from retinal atrophy (RA) and showing symptoms of progressive retinal atrophy (PRA), with the use of SD-OCT. The study was performed on 50 dogs divided into two groups: 25 dogs diagnosed with retinal atrophy (RA) with PRA symptoms aged 1.5-14 years and 25 healthy dogs aged 2-12 years. The dogs were examined using slit-lamp biomicroscopy, tonometry, ophthalmoscopy, fundus camera and SD-OCT (Topcon 3D OCT 2000). Measurements of the choroidal layers: RPE-Bruch membrane-choriocapillaris complex (RPE-BmCc) with tapetum lucidum in tapetal fundus, medium-sized vessel layer, (MSVL), large vessel layer with lamina suprachoroidea and (LVLS) whole choroidal thickness (WCT) were taken manually with the use of the caliper function integrated with the SD-OCT software. The measurements were performed dorsally (D) and ventrally (V) at a distance of 5000-6000 μm, and temporally (T) and nasally (N) at a distance of 4000-7000 μm from the optic disc with enhanced depth scans. The measurements were conducted temporally and nasally both in the tapetal (TempT, Nast) and nontapetal (TempNT, NasNT) fundus. Statistical analysis was performed using Statistica 10 software (Mann Whitney U Test). In all dogs affected by retinal atrophy (RA) with PRA symptoms, a statistically significant (p≤0.05) reduction in thickness of MSVL was observed in all the measured regions. A statistically significant reduction in thickness of LVLS and WCT was found in all nontapetal areas (p≤0.05). RA in mixed breed dogs with PRA symptoms was accompanied by choroid disorders such as reduction in thickness of the large vessel layer and decreased whole choroidal thickness in the nontapetal fundus as well as the medium vessel layer in all fundus regions.

本研究的目的是利用SD-OCT测定患有视网膜萎缩(RA)并表现进行性视网膜萎缩(PRA)症状的混合品种犬的脉络膜层厚度。该研究将50只狗分为两组:25只被诊断为视网膜萎缩(RA)并伴有PRA症状的狗,年龄在1.5-14岁之间,25只健康狗,年龄在2-12岁之间。采用裂隙灯生物显微镜、眼压计、眼底镜、SD-OCT (Topcon 3D OCT 2000)检查。脉络膜层的测量:使用与SD-OCT软件集成的卡尺功能,手动测量带绒底透明膜的RPE-Bruch膜-绒毛膜复合体(RPE-BmCc)、中等血管层(MSVL)、带脉络膜上膜的大血管层(LVLS)和全脉络膜厚度(WCT)。在距离视盘5000 ~ 6000 μm的位置进行背侧(D)和腹侧(V)测量,在距离视盘4000 ~ 7000 μm的位置进行颞侧(T)和鼻侧(N)测量,增强深度扫描。在毡底(TempT, Nast)和非毡底(TempNT, NasNT)进行时间和鼻腔测量。统计学分析采用Statistica 10软件(Mann Whitney U Test)。在所有伴有PRA症状的视网膜萎缩(RA)犬中,所有测量区域的MSVL厚度均有统计学意义(p≤0.05)的减少。所有非绒毡区LVLS和WCT厚度均有统计学意义的降低(p≤0.05)。伴有RA症状的混种犬伴脉络膜紊乱,如无绒底大血管层厚度减少、全脉络膜厚度减少以及眼底各区域中血管层厚度减少。
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引用次数: 0
Treatment with mannose oligosaccharides reverses the intestinal injury in the acetylsalicylic acid-treated rat model. 甘露寡糖可逆转乙酰水杨酸模型大鼠肠道损伤。
Pub Date : 2024-06-01 DOI: 10.24425/pjvs.2024.149355
H Qiao, Y Chen, K Yang, J Wang, Y Chou, L Chen, Y Zhang, J Huang, E Duan, L Su

Mannose oligosaccharide (MOS) has been shown to promote animal growth, maintain intestinal health, and activate the intestinal immune system. However, the question of whether MOS can stimulate the immune system and alleviate acetylsalicylic acid (ASA)-induced gut damage remains unresolved. The purpose of this study was to investigate the impact of MOS pretreatment on the immunological and anti-inflammatory capabilities of rats with ASA-induced intestinal injury. Thirty-six male Sprague-Dawley rats were divided into 6 groups and were fed with 0 (negative control), 100, 300, 600, and 800 mg/kg·Body weight (BW) of MOS for 3 weeks. From day 8, rats were fed with 200 mg/kg BW of ASA for 14 days to induce intestinal injury. The growth performance, viscera index, serum and intestinal immunity, intestinal inflammation and morphology of ASA-induced intestinal injury rats with or without MOS administration were investigated. In MOS deficient rats, oral treatment of ASA causes severe intestine damage and immunological dysfunction. In a rat model, 600 mg/kg BW MOS can lower the expression of inflammatory markers and effectively increase liver index, serum interleukin-2 (IL-2), lysozyme contents, intestinal secretory immunoglobulin A (sIgA) and mucus volume, intestinal villus height, crypt depth and villus height/crypt depth in comparison to the ASA group. These results imply that providing rats with MOS at the appropriate dosage can significantly improve their immune system and successfully shield the intestines from ASA damage. MOS is therefore expected to be a promising gut immunopotentiator for enhancing intestinal health in animals.

甘露寡糖(MOS)已被证明具有促进动物生长、维持肠道健康和激活肠道免疫系统的作用。然而,MOS是否能刺激免疫系统并减轻乙酰水杨酸(ASA)诱导的肠道损伤的问题仍未解决。本研究旨在探讨MOS预处理对asa诱导的肠损伤大鼠免疫和抗炎能力的影响。将36只雄性sd - dawley大鼠分为6组,分别饲喂0(阴性对照)、100、300、600、800 mg/kg·体重(BW)的MOS 3周。从第8天开始,给大鼠喂食200 mg/kg BW的ASA,连续14 d诱导肠道损伤。观察加、不加MOS的asa诱导肠损伤大鼠的生长性能、脏器指数、血清和肠道免疫、肠道炎症及形态学变化。在MOS缺陷大鼠中,口服ASA可引起严重的肠道损伤和免疫功能障碍。在大鼠模型中,与ASA组相比,600 mg/kg BW MOS可降低炎症标志物的表达,有效提高肝脏指数、血清白细胞介素-2 (IL-2)、溶菌酶含量、肠道分泌免疫球蛋白a (sIgA)和黏液体积、肠绒毛高度、隐窝深度和绒毛高度/隐窝深度。上述结果提示,适当剂量的MOS可以显著改善大鼠的免疫系统,并成功地保护肠道免受ASA损伤。因此,MOS有望成为一种有前途的肠道免疫增强剂,用于改善动物肠道健康。
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引用次数: 0
Effect of dietary supplementation of jujube fruit (Ziziphus jujuba) powder on performance, some biochemical parameters, and egg quality in quails (Coturnix coturnix japonica). 日粮中添加红枣粉对鹌鹑生产性能、部分生化指标及蛋品质的影响
Pub Date : 2024-06-01 DOI: 10.24425/pjvs.2024.149351
M Eroglu, M Ciftci, U G Simsek, S Akarsu, S Arslan, M A Hames, M Mutlu

This study aimed to evaluate the effects of different dietary supplementation levels with jujube fruit powder on the performance, biochemical parameters, and egg quality characteristics of laying quails. A total of 60 quails (45 days old) were randomly assigned to treatments with different levels of jujube fruit powder: a basal diet (control) and diets supplemented with 5 g/kg (T1), 10 g/kg (T2), with five replicates per treatment (20 quails/treatment and four quails/replicate). The differences between 1-15 and 16-30 days for feed intake (p<0.05), 1-60 days for egg production (p<0.05), 16-30 days for egg weight (p<0.05), and 1-15 and 1-60 days for feed conversion ratio were statistically significant. The highest values for egg width (p<0.01) and egg length (p<0.05) were found in the control group (25.87 and 33.55 mm), while lower values were observed in the T1 and T2 groups. There was no statistical difference between the groups in egg shape index, shell weight, shell ratio, and shell thickness (p>0.05). The yolk height (p<0.05) and yolk diameter (p<0.01) were statistically significant. The differences between the groups for all serum biochemical parameters were insignificant (p>0.05). According to the results of this study, jujube fruit, a rich energy source, can be used as an alternative supplement in poultry diets with positive effects on egg production and feed conversion ratio.

本试验旨在评价饲粮中添加不同水平红枣果粉对产蛋鹌鹑生产性能、生化指标及蛋品质特性的影响。试验选用45日龄的60只鹌鹑,随机分为基础饲粮(对照)和在基础饲粮中添加5 g/kg (T1)、10 g/kg (T2)的试验饲粮,每个处理5个重复(20只/处理,4只/重复)。采食量在1-15天和16-30 d之间差异显著(p0.05)。蛋黄高度(p0.05)。综上所述,红枣果实能量丰富,可作为家禽饲粮的替代添加物,对产蛋率和饲料系数均有积极影响。
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引用次数: 0
Evaluation of calcium, phosphorus and some biochemical parameters in dogs with open and closed cervix pyometra. 开放性和闭合性子宫颈脓脓症犬的钙、磷及一些生化指标的评价。
Pub Date : 2024-06-01 DOI: 10.24425/pjvs.2024.149354
I Kurban, S İslamoğlu, Z Günay Uçmak, E Namlı, Y S Türkan

The aim of this study was to evaluate the changes in calcium, phosphorus and some biochemical parameters in dogs with open and closed cervix pyometra, which was then compared with a control group. A total of 62 bitches of age group 5-10 years old irrespective of breed were enrolled into the study. Control group consisted of 22 bitches which were clinically healthy and in luteal phase of the estrus cycle. On clinical examination, pyometra was diagnosed in 40 bitches while 23 out of 40 bitches had open-cervix pyometra and 17 of 40 bitches had closed-cervix pyometra. Evaluation of haematological changes revealed that there was increased white blood cell (WBC), total protein (TP), globulin, alkaline phosphatase (ALKP) while decreased glucose (Glu), albumin (Alb) and albumin globulin ratio in pyometra cases (p<0.001). The highest WBC and ALKP levels were detected in closed-cervix pyometra group (p<0.001). Calcium (Ca+2) levels had significant rise in open-cervix pyometra cases (p<0.05) while phosphorus (Phos) levels had tendency to increase in closed-cervix pyometra cases (p=0.08). Also, Ca was directly correlated with Glu, Alb and TP while inversely correlated with WBC and blood urea nitrogen/creatinine (BUN/Crea). However, Phos was significantly associated with BUN and Crea (p<0.001; R=0.915 and R=0.860, respectively). As a result of this study, it was observed that the increased Ca levels in open-cervix cases and the elevated Phos levels in closed-cervix cases, were associated with the biochemical parameters related to hepatic and renal dysfunction in dogs with pyometra.

本研究的目的是评价开放式和闭合性宫颈脓潴留犬的钙、磷及一些生化参数的变化,并与对照组进行比较。试验共选取62只5 ~ 10岁的母犬,不分品种。对照组为临床健康、处于发情周期黄体期的公犬22只。在临床检查中,40只母狗诊断为子宫积脓,40只母狗中有23只为开宫颈积脓,40只母狗中有17只为闭宫颈积脓。血液学变化的评估显示,脓脓患者的白细胞(WBC)、总蛋白(TP)、球蛋白、碱性磷酸酶(ALKP)升高,而葡萄糖(Glu)、白蛋白(Alb)和白蛋白-球蛋白比值降低(p
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引用次数: 0
First detection of Ehrlichia chaffeensis, Ehrlichia canis, and Anaplasma ovis in Rhipicephalus bursa ticks collected from sheep, Turkey. 首次在从土耳其羊身上采集到的包囊蜱中检测到埃利希氏菌、犬埃利希氏菌和弓形虫。
Pub Date : 2024-03-20 DOI: 10.24425/pjvs.2024.149338
A Ayan, B Aslan Çelik, Ö Y Çelik, Ö Orunç Kılınç, G Akyıldız, A B Yılmaz, D N Sayın İpek, Ö Oktay Ayan, A R Babaoğlu

Anaplasmosis and ehrlichiosis are important tick-borne rickettsial diseases of medical and veterinary importance that cause economic losses in livestock. In this study, the prevalence of Anaplasma ovis, Ehrlichia canis and Ehrlichia chaffeensis was investigated in ticks collected from sheep in various farms in Van province, which is located in the Eastern Anatolian Region of Turkey. The ticks used in this study were collected by random sampling in 26 family farm business in 13 districts of Van province. A total of 688 ticks were collected from 88 sheep and 88 tick pools were created. All ticks identified morphologically as Rhipicephalus bursa. Phylogenetic analysis of Chaperonin and 16S rRNA gene sequences confirmed A. ovis, E. canis and E. chaffeensis in this study. Of the 88 tick pools tested, 28.41% (25/88) were positive for at least one pathogen. Anaplasma DNA was detected in five of the 88 pools (5.68%), E. canis DNA was detected in 19 of the 88 pools (21.59%), and E. chaffeensis DNA was detected in one of the 88 pools (1.14%) of R. bursa ticks. To our knowledge, this is the first report describing the presence of A. ovis, E. canis, and E. chaffeensis in R. bursa ticks collected from sheep in Turkey. Further studies are needed to investigate other co-infections in sheep in Turkey.

无形体病和埃立克病是重要的蜱传立克次体疾病,在医学和兽医学方面具有重要意义,会给牲畜造成经济损失。本研究调查了从土耳其东安纳托利亚地区凡省不同农场的羊身上采集的蜱虫中阿纳帕斯原虫、犬埃立克原虫和沙弗氏埃立克原虫的流行情况。本研究使用的蜱虫是在凡省 13 个区的 26 个家庭农场企业中随机抽样采集的。共从 88 只羊身上采集了 688 只蜱虫,并建立了 88 个蜱虫池。经形态鉴定,所有蜱虫均为法氏囊蜱(Rhipicephalus bursa)。该研究通过对 Chaperonin 和 16S rRNA 基因序列进行系统发育分析,确认了 A. ovis、E. canis 和 E. chaffeensis。在检测的 88 个蜱池中,28.41%(25/88)对至少一种病原体呈阳性。在 88 个蜱池中有 5 个(5.68%)检测到阿那普拉斯原虫 DNA,在 88 个蜱池中有 19 个(21.59%)检测到犬大肠杆菌 DNA,在 88 个囊蜱池中有 1 个(1.14%)检测到沙弗氏大肠杆菌 DNA。据我们所知,这是第一份描述从土耳其绵羊身上采集的囊蜱中存在 A. ovis、E. canis 和 E. chaffeensis 的报告。还需要进一步研究土耳其绵羊的其他合并感染情况。
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引用次数: 0
Vibrio infection in freshwater fish in Poland. 波兰淡水鱼中的弧菌感染。
Pub Date : 2024-03-20 DOI: 10.24425/pjvs.2024.149341
A Pastuszka, L Guz, K Michalak, D Pietras-Ożga, K Puk

Vibrio species are common inhabitants of aquatic environments and have been described in connection with fish and human diseases. Six Vibrio species were isolated from diseased freshwater and ornamental fish in Poland. The strains were identified based on morphological and biochemical characteristics and confirmed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) as V. albensis (n=3) from Gymnocephalus cernua, Sander lucioperca, Paracheirodon innesi, and Xiphophorus hellerii; V. mimicus (n=1) from Xiphophorus maculatus; and V. vulnificus (n=1) from Nematobrycon palmeri. This is the first time that Vibrio species have been isolated and described from ornamental fish in Poland. The isolates were resistant to ampicillin (83.3%), gentamicin (16.6%), ciprofloxacin (16.6%), sulfamethoxazole-trimethoprim (16.6%), and chloramphenicol (16.6%). The multiple antibiotic resistance (MAR) index was 0.00-0.08 for V. albensis, 0.17 for V. mimicus, and 0.33 for V. vulnificus. Our study confirmed the presence of potentially pathogenic Vibrio species in freshwater and ornamental fish. Therefore, further monitoring of the presence of Vibrio species, mainly in ornamental fish, is necessary.

弧菌是水生环境中的常见居民,曾被描述与鱼类和人类疾病有关。从波兰患病的淡水鱼和观赏鱼中分离出了六种弧菌。根据形态学和生化特征对这些菌株进行了鉴定,并通过基质辅助激光解吸/电离飞行时间质谱(MALDI-TOF-MS)确认为 V. albensis(n=3)、V.albensis (n=3) from Gymnocephalus cernua, Sander lucioperca, Paracheirodon innesi, and Xiphophorus hellerii; V. mimicus (n=1) from Xiphophorus maculatus; and V. vulnificus (n=1) from Nematobrycon palmeri.这是波兰首次从观赏鱼中分离并描述弧菌物种。这些分离物对氨苄西林(83.3%)、庆大霉素(16.6%)、环丙沙星(16.6%)、磺胺甲恶唑-三甲氧苄(16.6%)和氯霉素(16.6%)具有耐药性。白纹伊蚊的多重抗生素耐药性(MAR)指数为 0.00-0.08,模仿伊蚊为 0.17,弧菌为 0.33。我们的研究证实了淡水鱼和观赏鱼中存在潜在的致病弧菌。因此,有必要进一步监测弧菌的存在,主要是在观赏鱼中。
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引用次数: 0
Immunohistochemical properties of motoneurons supplying the porcine trapezius muscle. 供应猪斜方肌的运动神经元的免疫组化特性。
Pub Date : 2024-03-20 DOI: 10.24425/pjvs.2024.149337
A Dudek, W Sienkiewicz, E Lepiarczyk, J Kaleczyc

The trapezius muscle (TRAP) belongs to the scapulothoracic group of muscles, which play a crucial role in the integrity and strength of the upper limb, trunk, head, and neck movements and, thus, in maintaining balance. Combined retrograde tracing (using fluorescent tracer Fast Blue, FB) and double-labelling immunohistochemistry were applied to investigate the chemical coding of motoneurons projecting to the porcine TRAP. FB-positive (FB+) motoneurons supplying the cervical (c-TRAP) and thoracic part (th-TRAP) of the right (injected with the tracer) TRAP were located within the IX-th Rexed lamina in the ipsilateral ventral horn of the grey matter of the spinal medulla. Immunohistochemistry revealed that nearly all the neurons were cholinergic in nature [choline acetyltransferase (CHAT)- or vesicular acetylcholine transporter (VACHT)-positive]. Many retrogradelly labelled neurons displayed also immunoreactivity to calcitonin gene-related peptide (CGRP; approximately 68% of FB+ neurons). The smaller number of nerve cells (5%, 3%, 2% or 1%, respectively) stained for nitric oxide synthase (n-NOS), vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY) and substance P (SP). The retrogradely labelled neurons were closely apposed by nerve fibres expressing immunoreactivity to CHAT, VACHT, CGRP, SP, DβH, VIP, n-NOS, NPY, GAL, Leu-Enk and Met-Enk. Taking into account the clinical relevance of TRAP, the present results may be useful in designing further research aimed at the management of various dysfunctions of the muscle.

斜方肌(TRAP)属于肩胛胸肌群,对上肢、躯干、头部和颈部运动的完整性和强度以及保持平衡起着至关重要的作用。我们采用逆行追踪(使用荧光示踪剂快蓝,FB)和双标记免疫组织化学相结合的方法来研究投射到猪 TRAP 的运动神经元的化学编码。供应右侧(注射示踪剂)TRAP颈部(c-TRAP)和胸部(th-TRAP)的FB阳性(FB+)运动神经元位于脊髓灰质同侧腹角的IX-th Rexed薄层内。免疫组化显示,几乎所有的神经元都是胆碱能神经元[胆碱乙酰转移酶(CHAT)或囊泡乙酰胆碱转运体(VACHT)阳性]。许多逆行性标记的神经元还显示降钙素基因相关肽(CGRP,约占 FB+ 神经元的 68%)的免疫反应。较小数量的神经细胞(分别为 5%、3%、2% 或 1%)对一氧化氮合酶 (n-NOS)、血管活性肠肽 (VIP)、神经肽 Y (NPY) 和 P 物质 (SP) 进行了染色。逆行标记的神经元与表达 CHAT、VACHT、CGRP、SP、DβH、VIP、n-NOS、NPY、GAL、Leu-Enk 和 Met-Enk 免疫活性的神经纤维紧密相连。考虑到 TRAP 的临床相关性,本研究结果可能有助于设计旨在治疗各种肌肉功能障碍的进一步研究。
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引用次数: 0
Evaluation of uterine antioxidants in bitches suffering from cystic endometrial hyperplasia-pyometra complex. 对患有子宫内膜囊性增生-子宫脓肿综合症的母犬子宫抗氧化剂的评估
Pub Date : 2024-03-20 DOI: 10.24425/pjvs.2024.149332
A Kumar, J K Prasad, S Verma, A Gattani, G D Singh, V K Singh

Cystic endometrial hyperplasia-pyometra complex (CEH-P) is a common disease in sexually mature bitches. Disease progression leads to oxidative stress, resulting in the depletion of uterine antioxidants and lipid peroxidation of associated cells, which further aggravates the condition. The concentration of antioxidant enzymes, the level of lipid peroxidation within the uterine tissue, and its reflection in the serum and urine need to be elucidated. The aim of this study was to analyze the concentration of antioxidants such as superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), glutathione peroxidase (GPx), and the lipid peroxidation marker malonaldehyde (MDA) in three types of samples, i.e., serum, urine, and uterine tissue. For this purpose, 58 pyometra-affected and 44 healthy bitches were included in the present study. All animals underwent ovariohysterectomy (OVH). Our data indicated highly significant difference (p<0.01) in the antioxidant concentrations of uterine, serum and urine samples. Furthermore, there was a highly significant (p<0.01) difference in the serum levels of ferric reducing antioxidant power (FRAP) and free radical scavenging activity (FRSA) indicated poor capacity to overcome oxidative stress in the CEH-Pyometra condition. We showed that CEH-P induces oxidative stress, which further depletes the antioxidant enzyme reserves in the uterus. Thus, the weak antioxidant defence predisposes to uterine damage and disease progression. The simultaneous depletion of antioxidants and an increase in lipid peroxidation in the serum and urine may also act as early indicators of uterine pathology.

子宫内膜囊性增生-子宫脓肿(CEH-P)是性成熟母犬的一种常见疾病。疾病的发展会导致氧化应激,造成子宫抗氧化剂耗竭和相关细胞的脂质过氧化,从而进一步加重病情。抗氧化酶的浓度、子宫组织内脂质过氧化的水平及其在血清和尿液中的反映需要加以阐明。本研究旨在分析三种样本(即血清、尿液和子宫组织)中抗氧化剂的浓度,如超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GPx)和脂质过氧化标记物丙二醛(MDA)。为此,本研究纳入了 58 只受子宫脓肿影响的母犬和 44 只健康母犬。所有动物均接受了卵巢切除术(OVH)。我们的数据表明,两组动物的血清和尿液以及子宫组织存在非常显著的差异(p
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引用次数: 0
Effect of dressing with different antiseptic agents on reproductive parameters in rats undergoing abdominal incision. 用不同消毒剂包扎对腹部切口大鼠生殖参数的影响
Pub Date : 2024-03-20 DOI: 10.24425/pjvs.2024.149331
G D Akarsu, E Erbaş, S A Akarsu

Antiseptic agents used in the postoperative period affect the functions of many tissues in the body, including the testicles. In this study, the effect of dressings administered with different antiseptic agents on testicular functions in rats that underwent abdominal incisions was investigated. A total of 48 Sprague-Dawley rats were used in the study. Each of the rats in the study group underwent a 4 cm-long skin and muscle operation. The incision was then stitched immediately. Antiseptics, hemp seed oil, hemp leaf oil, and cannabidiol oil were then administered to the rats for 10 days to provide antisepsis. The rats were sacrificed 24 hours after the last administration, and testicular tissues were removed. Testicular tissues were used for histopathological examination and biochemical analysis, while epididymal tissue was used for sperm analysis. According to the results, the MDA level in the antiseptic-administered group was higher than in the other experimental groups (p<0.05). Levels of SOD, CAT activities, and GSH content were found to be lower in the antiseptic group than in the hemp seed oil, hemp leaf oil, and cannabidiol oil groups (p<0.05). In testicular histology, the SEED group had the highest Johnsen score, and the antiseptic group had the lowest score (p<0.05). While JAK, P-JAK2, STAT3, PSTAT3, and NF-κB were generally higher in the antiseptic group compared to the other groups, they were lower in the SEED group. Additionally, sperm total motility rate and epididymal sperm density were highest in the SEED group (p<0.05). As a result, it was determined that cannabidiol seed oil had a good effect on testicular histology and sperm quality in male rats during the wound healing process.

术后使用的防腐剂会影响体内许多组织的功能,包括睾丸。本研究调查了使用不同防腐剂的敷料对腹部切口大鼠睾丸功能的影响。研究共使用了 48 只 Sprague-Dawley 大鼠。研究组中的每只大鼠都接受了 4 厘米长的皮肤和肌肉手术。然后立即缝合切口。然后给大鼠注射防腐剂、大麻籽油、大麻叶油和大麻二酚油,为期 10 天,以起到防腐作用。大鼠在最后一次用药 24 小时后被处死,并取出睾丸组织。睾丸组织用于组织病理学检查和生化分析,附睾组织用于精子分析。结果显示,使用防腐剂组的 MDA 水平高于其他实验组(p
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引用次数: 0
Investigation of the protective effect of chitosan against arsenic-induced nephrotoxicity and oxidative damage in rat kidney tissue. 壳聚糖对砷引起的大鼠肾脏毒性和氧化损伤的保护作用研究
Pub Date : 2024-03-20 DOI: 10.24425/pjvs.2024.149339
K İrak, Ö Y Çelik, M Bolacalı, T Tufan, S Özcan, S Yıldırım, I Bolat

Arsenic is an important metalloid that can cause poisoning in humans and domestic animals. Exposure to arsenic causes cell damage, increasing the production of reactive oxygen species. Chitosan is a biopolymer obtained by deacetylation of chitin with antioxidant and metal ion chelating properties. In this study, the protective effect of chitosan on arsenic-induced nephrotoxicity and oxidative damage was investigated. 32 male Wistar-albino rats were divided into 4 groups of 8 rats each as control group (C), chitosan group (CS group), arsenic group (AS group), and arsenic+chitosan group (AS+CS group). The C group was given distilled water by oral gavage, the AS group was given 100 ppm/day Na-arsenite ad libitum with drinking water, the CS group was given 200 mg/kg/day chitosan dissolved in saline by oral gavage, the AS+CS group was given 100 ppm/day Na-arsenite ad libitum with drinking water and 200 mg/kg/day chitosan dissolved in saline by oral gavage for 30 days. At the end of the 30-day experimental period, 90 mg/kg ketamine was administered intraperitoneally to all rats, and blood samples and kidney tissues were collected. Urea, uric acid, creatinine, P, Mg, K, Ca, Na, Cystatin C (CYS-C), Neutrophil Gelatinase Associated Lipocalin (NGAL) and Kidney Injury Molecule 1 (KIM-1) levels were measured in serum samples. Malondialdehyde (MDA), Glutathione (GSH), Catalase (CAT) and Superoxide dismutase (SOD) levels in the supernatant obtained from kidney tissue were analyzed by ELISA method. Compared with AS group, uric acid and creatinine levels of the AS+CS group were significantly decreased (p<0.001), urea, KIM-1, CYS-C, NGAL, and MDA levels were numerically decreased and CAT, GSH, and SOD levels were numerically increased (p>0.05). In conclusion, based on both biochemical and histopathological-immunohistochemical- immunofluorescence findings, it can be concluded that chitosan attenuates kidney injury and protects the kidney.

砷是一种重要的类金属,可导致人类和家畜中毒。接触砷会导致细胞损伤,增加活性氧的产生。壳聚糖是一种由甲壳素脱乙酰化得到的生物聚合物,具有抗氧化和金属离子螯合特性。本研究探讨了壳聚糖对砷诱导的肾毒性和氧化损伤的保护作用。将 32 只雄性 Wistar-albino 大鼠分为 4 组,每组 8 只,分别为对照组(C 组)、壳聚糖组(CS 组)、砷组(AS 组)和砷+壳聚糖组(AS+CS 组)。C 组口服蒸馏水,AS 组每天在饮用水中自由添加 100 ppm 的砒霜,CS 组每天口服 200 毫克/千克溶于生理盐水的壳聚糖,AS+CS 组每天在饮用水中自由添加 100 ppm 的砒霜,每天口服 200 毫克/千克溶于生理盐水的壳聚糖,连续 30 天。在 30 天实验期结束时,对所有大鼠腹腔注射 90 毫克/千克氯胺酮,并采集血样和肾组织。测量血清样本中的尿素、尿酸、肌酐、P、Mg、K、Ca、Na、胱抑素 C(CYS-C)、中性粒细胞明胶酶相关脂褐质(NGAL)和肾损伤分子 1(KIM-1)的水平。用 ELISA 方法分析了肾组织上清液中丙二醛(MDA)、谷胱甘肽(GSH)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)的水平。与 AS 组相比,AS+CS 组的尿酸和肌酐水平明显下降(P0.05)。总之,根据生化和组织病理学-免疫组织化学-免疫荧光的研究结果,可以得出壳聚糖能减轻肾脏损伤并保护肾脏的结论。
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Polish journal of veterinary sciences
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