André Mattos Brito de Souza, N. L. G. Albuquerque, J. Mendonça, L. Rodrigues, S. L. Santiago
PURPOSE To evaluate the use of oxalic acid in restorations of noncarious cervical lesions. MATERIALS AND METHODS Twenty volunteers of both sexes between 24 and 55 years old, with at least two lesions that were restored with the techniques to be evaluated, were selected. Ninety teeth were randomly divided into two groups: group 1, two-step etch-and-rinse adhesive technique (control) and group 2, pretreatment with oxalic acid followed by the application of a two-step etch-and-rinse adhesive system. The adhesive system used was XP Bond (Dentsply) and the restorative composite resin was Durafill (Heraeus Kulzer). A modified USPHS method was employed for the clinical evaluation, taking into account the following criteria: retention (R), marginal integrity (MI), marginal discoloration (MD), postoperative sensitivity (S), caries (C), and anatomic form (AF). Clinical assessments were conducted by two examiners at baseline and after 6 months, 1 and 2 years. RESULTS After two years, the results of clinically satisfactory restorations (Alpha and Bravo) obtained for the control and experimental groups were: R (90.9%/93.33%), MI (100%/100%), MD (100%/100%), S (100%/100%), C (100%/100%), AF (100%/100%). CONCLUSION Over a 2-year period, the use of oxalic acid as an agent of dentin pretreatment did not influence the clinical performance of restorations in noncarious cervical lesions.
目的探讨草酸在宫颈良性病变修复中的应用。材料与方法选择了20名年龄在24 - 55岁之间的男女志愿者，他们至少有两个病变已经用待评估的技术修复了。90颗牙随机分为两组:1组采用两步蚀刻-冲洗粘接技术(对照组)，2组采用草酸预处理，然后应用两步蚀刻-冲洗粘接系统。使用的粘接系统为XP Bond (Dentsply)，修复复合树脂为Durafill (Heraeus Kulzer)。采用改良的USPHS方法进行临床评估，考虑以下标准:保留(R)、边缘完整性(MI)、边缘变色(MD)、术后敏感性(S)、龋齿(C)和解剖形态(AF)。两名检查人员分别在基线、6个月、1年和2年后进行临床评估。结果两年后，对照组和试验组临床满意的修复体(Alpha和Bravo)分别为R(90.9%/93.33%)、MI(100%/100%)、MD(100%/100%)、S(100%/100%)、C(100%/100%)、AF(100%/100%)。结论在2年的时间里，草酸作为牙本质预处理剂对宫颈非龋齿病变修复的临床效果没有影响。
{"title":"Randomized Two-year Clinical Evaluation of Oxalic Acid in Restorations of Noncarious Cervical Lesions.","authors":"André Mattos Brito de Souza, N. L. G. Albuquerque, J. Mendonça, L. Rodrigues, S. L. Santiago","doi":"10.3290/j.jad.a37041","DOIUrl":"https://doi.org/10.3290/j.jad.a37041","url":null,"abstract":"PURPOSE To evaluate the use of oxalic acid in restorations of noncarious cervical lesions. MATERIALS AND METHODS Twenty volunteers of both sexes between 24 and 55 years old, with at least two lesions that were restored with the techniques to be evaluated, were selected. Ninety teeth were randomly divided into two groups: group 1, two-step etch-and-rinse adhesive technique (control) and group 2, pretreatment with oxalic acid followed by the application of a two-step etch-and-rinse adhesive system. The adhesive system used was XP Bond (Dentsply) and the restorative composite resin was Durafill (Heraeus Kulzer). A modified USPHS method was employed for the clinical evaluation, taking into account the following criteria: retention (R), marginal integrity (MI), marginal discoloration (MD), postoperative sensitivity (S), caries (C), and anatomic form (AF). Clinical assessments were conducted by two examiners at baseline and after 6 months, 1 and 2 years. RESULTS After two years, the results of clinically satisfactory restorations (Alpha and Bravo) obtained for the control and experimental groups were: R (90.9%/93.33%), MI (100%/100%), MD (100%/100%), S (100%/100%), C (100%/100%), AF (100%/100%). CONCLUSION Over a 2-year period, the use of oxalic acid as an agent of dentin pretreatment did not influence the clinical performance of restorations in noncarious cervical lesions.","PeriodicalId":94234,"journal":{"name":"The journal of adhesive dentistry","volume":"28 1","pages":"467-473"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82513839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Marcondes, N. Souza, F. B. Manfroi, L. H. Burnett, A. Spohr
PURPOSE To clinically evaluate the performance of indirect composite resin restorations cemented with conventional and self-adhesive resin cements over a 12-month period. MATERIALS AND METHODS Ten patients fulfilled all the inclusion criteria. Twenty-four composite resin restorations were performed using an indirect technique and cemented with a resin cement (RelyX ARC) or a self-adhesive resin cement (RelyX U100). Two independent evaluators analyzed the restorations using modified USPHS criteria after periods of two weeks and 6 and 12 months. Statistical significance between the cements at each timepoint was evaluated with the Wilcoxon test and between timepoints with the Mann-Whitney test, both at a significance level of 5%. Fisher's exact test was used to assess the occurrence of absolute failures. RESULTS No statistically significant differences were found between the groups at the same timepoint nor between groups at different timepoints. The only significant difference was found for color match for both groups after 12 months. CONCLUSION After 12 months, indirect composite resin restorations cemented with self-adhesive resin cement performed similarly to those cemented with conventional resin cement.
{"title":"Clinical Evaluation of Indirect Composite Resin Restorations Cemented with Different Resin Cements.","authors":"M. Marcondes, N. Souza, F. B. Manfroi, L. H. Burnett, A. Spohr","doi":"10.3290/j.jad.a35519","DOIUrl":"https://doi.org/10.3290/j.jad.a35519","url":null,"abstract":"PURPOSE To clinically evaluate the performance of indirect composite resin restorations cemented with conventional and self-adhesive resin cements over a 12-month period. MATERIALS AND METHODS Ten patients fulfilled all the inclusion criteria. Twenty-four composite resin restorations were performed using an indirect technique and cemented with a resin cement (RelyX ARC) or a self-adhesive resin cement (RelyX U100). Two independent evaluators analyzed the restorations using modified USPHS criteria after periods of two weeks and 6 and 12 months. Statistical significance between the cements at each timepoint was evaluated with the Wilcoxon test and between timepoints with the Mann-Whitney test, both at a significance level of 5%. Fisher's exact test was used to assess the occurrence of absolute failures. RESULTS No statistically significant differences were found between the groups at the same timepoint nor between groups at different timepoints. The only significant difference was found for color match for both groups after 12 months. CONCLUSION After 12 months, indirect composite resin restorations cemented with self-adhesive resin cement performed similarly to those cemented with conventional resin cement.","PeriodicalId":94234,"journal":{"name":"The journal of adhesive dentistry","volume":"20 4","pages":"59-67"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91491456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fangfang Sun, Peng Mao, Cong Wang, Chaowen Shi, Rongrong Nie, Ningning Han, Xiaodong Han
PURPOSE To evaluate the potential cytotoxic effects of four one-step self-etching dental adhesives [Adper Easy One (AEO), iBond (IB), Clearfil S³ Bond (CSB), and G-Bond (GB)] on cultured human periodontal ligament fibroblasts. MATERIALS AND METHODS Cured adhesives were immersed in complete DMEM or deionized water and maintained at 37°C for 24 h, followed by sterilization. The deionized water-based extract was used for Fourier transform infrared spectroscopy analysis. The DMEM-based extract was diluted into various concentrations for cytotoxicity tests. The viability, integrity, and apoptosis of cultured human periodontal ligament fibroblasts upon treatment with the extracts were determined using the CCK-8 assay, microscopy, and flow cytometry. RESULTS All of the four adhesives induced cell viability loss, cell morphology alteration, and cell death. GB showed the greatest cytotoxicity by inducing cell apoptosis and necrosis, while IB had the weakest cytotoxic effect on the cultured cells. CONCLUSION All tested dental adhesives have significant adverse effects on cell viability. Therefore, precautions should be taken to protect the periodontal tissues when dental adhesives are applied in the clinic.
目的探讨四种一步自蚀牙胶粘剂[Adper Easy One (AEO)、iBond (IB)、Clearfil S³Bond (CSB)和G-Bond (GB)]对培养的人牙周韧带成纤维细胞的潜在细胞毒性作用。材料与方法将固化的胶粘剂浸泡在完全的DMEM或去离子水中，在37°C下保持24 h，然后灭菌。用去离子水基提取物进行傅里叶变换红外光谱分析。将dmem提取液稀释成不同浓度进行细胞毒性试验。用CCK-8法、显微镜和流式细胞术检测培养的人牙周韧带成纤维细胞的活力、完整性和凋亡情况。结果4种胶粘剂均可引起细胞活力丧失、细胞形态改变和细胞死亡。GB诱导细胞凋亡和坏死的细胞毒性最大，而IB对培养细胞的细胞毒性作用最弱。结论所有牙粘接剂对细胞活力均有明显的不良影响。因此，在临床使用牙黏剂时，应采取预防措施保护牙周组织。
{"title":"Cytotoxic Effects of One-step Self-etching Dental Adhesives on Human Periodontal Ligament Fibroblasts In Vitro.","authors":"Fangfang Sun, Peng Mao, Cong Wang, Chaowen Shi, Rongrong Nie, Ningning Han, Xiaodong Han","doi":"10.3290/j.jad.a35906","DOIUrl":"https://doi.org/10.3290/j.jad.a35906","url":null,"abstract":"PURPOSE To evaluate the potential cytotoxic effects of four one-step self-etching dental adhesives [Adper Easy One (AEO), iBond (IB), Clearfil S³ Bond (CSB), and G-Bond (GB)] on cultured human periodontal ligament fibroblasts. MATERIALS AND METHODS Cured adhesives were immersed in complete DMEM or deionized water and maintained at 37°C for 24 h, followed by sterilization. The deionized water-based extract was used for Fourier transform infrared spectroscopy analysis. The DMEM-based extract was diluted into various concentrations for cytotoxicity tests. The viability, integrity, and apoptosis of cultured human periodontal ligament fibroblasts upon treatment with the extracts were determined using the CCK-8 assay, microscopy, and flow cytometry. RESULTS All of the four adhesives induced cell viability loss, cell morphology alteration, and cell death. GB showed the greatest cytotoxicity by inducing cell apoptosis and necrosis, while IB had the weakest cytotoxic effect on the cultured cells. CONCLUSION All tested dental adhesives have significant adverse effects on cell viability. Therefore, precautions should be taken to protect the periodontal tissues when dental adhesives are applied in the clinic.","PeriodicalId":94234,"journal":{"name":"The journal of adhesive dentistry","volume":"41 1","pages":"99-109"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81318803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Meng-Ling Chiang, Sebastian Birlbauer, Yi-Fang Lo, V. Pitchika, A. Crispin, N. Ilie, R. Hickel, J. Kühnisch
PURPOSE This study investigated the influence of several methodological details on the shear bond strength (SBS) testing of pit and fissure sealants. The following variables were considered: type of enamel surfaces, prismatic vs aprismatic enamel, etching time, and aging and shearing procedures. MATERIALS AND METHODS The surfaces of 180 healthy human third molars were divided into 4 different sections (mesial, distal, buccal, and oral). After tooth separation, the specimens were randomly allocated to the following groups: 1) enamel preparation: prismatic vs aprismatic; 2) etching: 30 s vs 60 s; 3) aging: 1 day or 3 months vs 5000 thermocycles; 4) shearing: notched-edge method (ISO 29022:2013) vs knife-edge method. After following each protocol, SBS was determined using a universal testing machine, followed by failure mode analysis. Data were analysed using Mann-Whitney U-tests and regression analyses. RESULTS In the aprismatic enamel group, the longer etching time resulted in slightly, not statistically significantly higher SBS. When aging sealants on aprismatic enamel with different procedures, significantly lower SBS was found for 5000 thermocycles. In the case of aprismatic enamel etched for 60 s and sheared with the notched-edge blade, there was no significant difference between the aging procedures. Failure mode analysis showed adhesive failures to be predominant. Simple linear regression revealed that all of the included factors significantly influenced SBS. In a multiple linear regression model, the variables "aprismatic enamel" and "distal surface" were associated with a higher SBS; "5000 thermocycles" reduced SBS significantly. CONCLUSION Enamel grinding, aging method, and type of enamel surface significantly influenced the SBS.
{"title":"Which Factors Influence the Shear Bond Strength of Sealant Materials?","authors":"Meng-Ling Chiang, Sebastian Birlbauer, Yi-Fang Lo, V. Pitchika, A. Crispin, N. Ilie, R. Hickel, J. Kühnisch","doi":"10.3290/j.jad.a36917","DOIUrl":"https://doi.org/10.3290/j.jad.a36917","url":null,"abstract":"PURPOSE This study investigated the influence of several methodological details on the shear bond strength (SBS) testing of pit and fissure sealants. The following variables were considered: type of enamel surfaces, prismatic vs aprismatic enamel, etching time, and aging and shearing procedures. MATERIALS AND METHODS The surfaces of 180 healthy human third molars were divided into 4 different sections (mesial, distal, buccal, and oral). After tooth separation, the specimens were randomly allocated to the following groups: 1) enamel preparation: prismatic vs aprismatic; 2) etching: 30 s vs 60 s; 3) aging: 1 day or 3 months vs 5000 thermocycles; 4) shearing: notched-edge method (ISO 29022:2013) vs knife-edge method. After following each protocol, SBS was determined using a universal testing machine, followed by failure mode analysis. Data were analysed using Mann-Whitney U-tests and regression analyses. RESULTS In the aprismatic enamel group, the longer etching time resulted in slightly, not statistically significantly higher SBS. When aging sealants on aprismatic enamel with different procedures, significantly lower SBS was found for 5000 thermocycles. In the case of aprismatic enamel etched for 60 s and sheared with the notched-edge blade, there was no significant difference between the aging procedures. Failure mode analysis showed adhesive failures to be predominant. Simple linear regression revealed that all of the included factors significantly influenced SBS. In a multiple linear regression model, the variables \"aprismatic enamel\" and \"distal surface\" were associated with a higher SBS; \"5000 thermocycles\" reduced SBS significantly. CONCLUSION Enamel grinding, aging method, and type of enamel surface significantly influenced the SBS.","PeriodicalId":94234,"journal":{"name":"The journal of adhesive dentistry","volume":"66 1","pages":"397-404"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81570438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Amaral, Alice Marques Diniz, Eugênio Braz Rodrigues Arantes, G. B. dos Santos, J. D. Noronha-Filho, E. M. da Silva
PURPOSE To investigate the influence of 4-META concentration and type of solvent on the degree of conversion (DC%) and resin-dentin bond stability of experimental etch-and-rinse adhesives. MATERIALS AND METHODS Four different concentrations of 4-META (12 wt%, 20 wt%, 30 wt%, 40 wt%) were added to a model adhesive system consisting of TEG-DMA (25 wt%), UDMA (20 wt%), HEMA (30 wt%), water (4 wt%), camphorquinone (0.5 wt%), and tertiary amine (0.5 wt%) dissolved in 20% acetone (A12, A20, A30 and A40) or 20% ethanol (E12, E20, E30 and E40). DC% was evaluated by FT-IR spectroscopy. Human molars were wet ground until the occlusal dentin was exposed, the adhesive systems were applied after 37% phosphoric acid etching, and resin composite buildups were incrementally constructed. After storage in distilled water at 37°C for 24 h, the teeth were cut into resin-dentin beams (cross-sectional area 1 mm2). Microtensile bond strength (μTBS) was evaluated after 24 h, 6 months, and 1 year of water storage at 37°C. The failure mode was categorized as adhesive, mixed, or cohesive. Data were analyzed using ANOVA and Tukey's HSD test (α = 0.05). RESULTS A12 presented the lowest DC% (p < 0.05). All the other adhesive systems showed statistically similar DC% (p > 0.05). All adhesive systems maintained resin-dentin bond stability after 6 months of water storage, while only A40 and E40 maintained it after 1 year. CONCLUSION Irrespective of the type of organic solvent, the incorporation of high concentrations of 4-META (40 wt%) improved the resin-dentin bond stability of the experimental etch-and-rinse adhesive systems over a period of 1 year.
{"title":"Resin-dentin Bond Stability of Experimental 4-META-based Etch-and-rinse Adhesives Solvated by Ethanol or Acetone.","authors":"C. Amaral, Alice Marques Diniz, Eugênio Braz Rodrigues Arantes, G. B. dos Santos, J. D. Noronha-Filho, E. M. da Silva","doi":"10.3290/j.jad.a37200","DOIUrl":"https://doi.org/10.3290/j.jad.a37200","url":null,"abstract":"PURPOSE To investigate the influence of 4-META concentration and type of solvent on the degree of conversion (DC%) and resin-dentin bond stability of experimental etch-and-rinse adhesives. MATERIALS AND METHODS Four different concentrations of 4-META (12 wt%, 20 wt%, 30 wt%, 40 wt%) were added to a model adhesive system consisting of TEG-DMA (25 wt%), UDMA (20 wt%), HEMA (30 wt%), water (4 wt%), camphorquinone (0.5 wt%), and tertiary amine (0.5 wt%) dissolved in 20% acetone (A12, A20, A30 and A40) or 20% ethanol (E12, E20, E30 and E40). DC% was evaluated by FT-IR spectroscopy. Human molars were wet ground until the occlusal dentin was exposed, the adhesive systems were applied after 37% phosphoric acid etching, and resin composite buildups were incrementally constructed. After storage in distilled water at 37°C for 24 h, the teeth were cut into resin-dentin beams (cross-sectional area 1 mm2). Microtensile bond strength (μTBS) was evaluated after 24 h, 6 months, and 1 year of water storage at 37°C. The failure mode was categorized as adhesive, mixed, or cohesive. Data were analyzed using ANOVA and Tukey's HSD test (α = 0.05). RESULTS A12 presented the lowest DC% (p < 0.05). All the other adhesive systems showed statistically similar DC% (p > 0.05). All adhesive systems maintained resin-dentin bond stability after 6 months of water storage, while only A40 and E40 maintained it after 1 year. CONCLUSION Irrespective of the type of organic solvent, the incorporation of high concentrations of 4-META (40 wt%) improved the resin-dentin bond stability of the experimental etch-and-rinse adhesive systems over a period of 1 year.","PeriodicalId":94234,"journal":{"name":"The journal of adhesive dentistry","volume":"244 1","pages":"513-520"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74531502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Takamizawa, W. Barkmeier, A. Tsujimoto, H. Endo, K. Tsuchiya, R. Erickson, M. Latta, M. Miyazaki
PURPOSE To use shear bond strength (SBS) and shear fatigue strength (SFS) testing to determine the influence of phosphoric acid pre-etching times prior to application of self-etch adhesives on enamel bonding. MATERIALS AND METHODS Two single-step self-etch universal adhesives (Prime&Bond Elect and Scotchbond Universal), a conventional single-step self-etch adhesive (G-ӕnial Bond), and a conventional two-step self-etch adhesive (OptiBond XTR) were used. The SBS and SFS were obtained with phosphoric acid pre-etching for 3, 10, or 15 s prior to application of the adhesives, and without pre-etching (0 s) as a control. A staircase method was used to determine the SFS with 10 Hz frequency for 50,000 cycles or until failure occurred. The mean demineralization depth for each treated enamel surface was also measured using a profilometer. RESULTS For all the adhesives, the groups with pre-etching showed significantly higher SBS and SFS than groups without pre-etching. However, there was no significant difference in SBS and SFS among groups with > 3 s of preetching. In addition, although the groups with pre-etching showed significantly deeper demineralization depths than groups without pre-etching, there was no significant difference in depth among groups with > 3 s of pre-etching. CONCLUSION Three seconds of phosphoric acid pre-etching prior to application of self-etch adhesive can enhance enamel bonding effectiveness.
{"title":"Influence of Pre-etching Times on Fatigue Strength of Self-etch Adhesives to Enamel.","authors":"T. Takamizawa, W. Barkmeier, A. Tsujimoto, H. Endo, K. Tsuchiya, R. Erickson, M. Latta, M. Miyazaki","doi":"10.3290/j.jad.a37361","DOIUrl":"https://doi.org/10.3290/j.jad.a37361","url":null,"abstract":"PURPOSE To use shear bond strength (SBS) and shear fatigue strength (SFS) testing to determine the influence of phosphoric acid pre-etching times prior to application of self-etch adhesives on enamel bonding. MATERIALS AND METHODS Two single-step self-etch universal adhesives (Prime&Bond Elect and Scotchbond Universal), a conventional single-step self-etch adhesive (G-ӕnial Bond), and a conventional two-step self-etch adhesive (OptiBond XTR) were used. The SBS and SFS were obtained with phosphoric acid pre-etching for 3, 10, or 15 s prior to application of the adhesives, and without pre-etching (0 s) as a control. A staircase method was used to determine the SFS with 10 Hz frequency for 50,000 cycles or until failure occurred. The mean demineralization depth for each treated enamel surface was also measured using a profilometer. RESULTS For all the adhesives, the groups with pre-etching showed significantly higher SBS and SFS than groups without pre-etching. However, there was no significant difference in SBS and SFS among groups with > 3 s of preetching. In addition, although the groups with pre-etching showed significantly deeper demineralization depths than groups without pre-etching, there was no significant difference in depth among groups with > 3 s of pre-etching. CONCLUSION Three seconds of phosphoric acid pre-etching prior to application of self-etch adhesive can enhance enamel bonding effectiveness.","PeriodicalId":94234,"journal":{"name":"The journal of adhesive dentistry","volume":"33 1","pages":"501-511"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74622107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Editorial: The \"etch-and-rinse\" and \"self-etch\" camps.","authors":"B. Van Meerbeek, R. Frankenberger","doi":"10.3290/j.jad.a37487","DOIUrl":"https://doi.org/10.3290/j.jad.a37487","url":null,"abstract":"","PeriodicalId":94234,"journal":{"name":"The journal of adhesive dentistry","volume":"37 1","pages":"463"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81822746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
PURPOSE To establish a fluorescence-based method to simultaneously assess micro-permeability and collagen cross-linking induced by chemical agents at the resin-dentin interface. MATERIALS AND METHODS Three chemical agents were investigated (proanthocyanidin-rich grape seed extract: GSE; carbodiimide hydrochloride/N-hydroxysuccinimide: EDC/NHS; glutaraldehyde: GD) along with a control (distilled water) as primers applied on flat occlusal dentin surfaces of 48 teeth and restored with two commercially available etch-and-rinse adhesives. Resin-dentin interfaces were polished and infiltrated with rhodamine-B solution for confocal laser scanning microscopy analysis. Parameters were chosen that would allow acquisition of a simultaneous appearance of collagen and interfacial micro-permeability (rhodamine-B). Fluorescence emission intensity (FEI) was converted into numerals and values were calculated for each group. Data were statistically analyzed using one-way ANOVA and post-hoc Scheffe's and multiple comparisons tests (α = 0.05). T-tests with Pearson correlations were used to investigate correlations between collagen cross-linking and micro-permeability. RESULTS The FEI of collagen was the highest for GD, followed by GSE, with no significant differences between EDC/ NHS and the control group (p > 0.05). Micro-permeability was significantly affected by the adhesives (p < 0.05). Micro- permeability was the lowest for GSE groups, regardless of the adhesives (p < 0.001). Weak correlations were found between micro-permeability and collagen auto-fluorescence. CONCLUSIONS Non-enzymatic collagen cross-linking induced by GSE and GD can be detected by increased collagen auto-fluorescence, and results in reduced interfacial micro-permeability. Increased collagen auto-fluorescence was correlated with fluorescent collagen cross-links and decreased micro-permeability at the resin-dentin interface. Collagen auto-fluorescence is a useful tool to detect auto-fluorescent exogenous cross links and their potential impact on the quality of the resin-dentin interface.
{"title":"Assessing Collagen and Micro-permeability at the Proanthocyanidin-treated Resin-Dentin Interface.","authors":"B. Aydın, L. Hassan, G. Viana, A. Bedran-Russo","doi":"10.3290/j.jad.a37359","DOIUrl":"https://doi.org/10.3290/j.jad.a37359","url":null,"abstract":"PURPOSE To establish a fluorescence-based method to simultaneously assess micro-permeability and collagen cross-linking induced by chemical agents at the resin-dentin interface. MATERIALS AND METHODS Three chemical agents were investigated (proanthocyanidin-rich grape seed extract: GSE; carbodiimide hydrochloride/N-hydroxysuccinimide: EDC/NHS; glutaraldehyde: GD) along with a control (distilled water) as primers applied on flat occlusal dentin surfaces of 48 teeth and restored with two commercially available etch-and-rinse adhesives. Resin-dentin interfaces were polished and infiltrated with rhodamine-B solution for confocal laser scanning microscopy analysis. Parameters were chosen that would allow acquisition of a simultaneous appearance of collagen and interfacial micro-permeability (rhodamine-B). Fluorescence emission intensity (FEI) was converted into numerals and values were calculated for each group. Data were statistically analyzed using one-way ANOVA and post-hoc Scheffe's and multiple comparisons tests (α = 0.05). T-tests with Pearson correlations were used to investigate correlations between collagen cross-linking and micro-permeability. RESULTS The FEI of collagen was the highest for GD, followed by GSE, with no significant differences between EDC/ NHS and the control group (p > 0.05). Micro-permeability was significantly affected by the adhesives (p < 0.05). Micro- permeability was the lowest for GSE groups, regardless of the adhesives (p < 0.001). Weak correlations were found between micro-permeability and collagen auto-fluorescence. CONCLUSIONS Non-enzymatic collagen cross-linking induced by GSE and GD can be detected by increased collagen auto-fluorescence, and results in reduced interfacial micro-permeability. Increased collagen auto-fluorescence was correlated with fluorescent collagen cross-links and decreased micro-permeability at the resin-dentin interface. Collagen auto-fluorescence is a useful tool to detect auto-fluorescent exogenous cross links and their potential impact on the quality of the resin-dentin interface.","PeriodicalId":94234,"journal":{"name":"The journal of adhesive dentistry","volume":"20 1","pages":"529-534"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75684175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
PURPOSE To evaluate the fracture strength and the failure mode of endodontically treated teeth restored with composite resin overlays with and without glass-fiber reinforcement. MATERIALS AND METHODS A total of 32 extracted molars were divided into four equal groups. In the NFR-NFRC (no foundation restoration, no fiber-reinforced composite) and NFR-FRC (no foundation restoration, fiber-reinforced composite) groups, only a 5-mm-thick composite resin layer sealed the pulp chamber floors, whereas in the FR-NFRC (foundation restoration, no fiber-reinforced composite) and FR-FRC (foundation restoration, fiber-reinforced composite) groups, a 3.0-mm foundation restoration was used. NFR-NFRC and FR-NFRC groups were restored with composite resin overlays, whereas NFR-FRC and FR-FRC groups were restored with fiber-reinforced composite resin overlays. All specimens were subjected to mechanical loading in a computer-controlled masticator and then the fracture resistance was evaluated. Differences in means were compared using two-way ANOVA and Tukey's test. The level of significance was set at ɑ = 0.05. RESULTS All specimens successfully completed the fatigue test. The least fracture-resistant group was NFR-FRC, exceeded by FR-NFRC, NFR-NFRC, and FR-FRC, in that order, with FR-FRC being the most fracture-resistant group. Statistically significant differences were detected between the pairs NFR-NFRC/FR-FRC (p = 0.001), NFR-FRC/FR-FRC (p = 0.001), and FR-NFRC/FR-FRC (p = 0.001). Eight vertical root fractures occurred in group FR-NFRC, six in group NFR-NFRC, four in group NFR-FRC, and none occurred in group FR-FRC. CONCLUSIONS Within the limitations of this in vitro study, the incorporation of glass fibers and the presence of a foundation restoration were found to increase the fracture resistance and can favorably influence the fracture mode.
{"title":"Fracture Strength of Endodontically Treated Teeth Restored with Composite Overlays with and without Glass-fiber Reinforcement.","authors":"C. Monaco, A. Arena, R. Scotti, I. Krejci","doi":"10.3290/j.jad.a35908","DOIUrl":"https://doi.org/10.3290/j.jad.a35908","url":null,"abstract":"PURPOSE To evaluate the fracture strength and the failure mode of endodontically treated teeth restored with composite resin overlays with and without glass-fiber reinforcement. MATERIALS AND METHODS A total of 32 extracted molars were divided into four equal groups. In the NFR-NFRC (no foundation restoration, no fiber-reinforced composite) and NFR-FRC (no foundation restoration, fiber-reinforced composite) groups, only a 5-mm-thick composite resin layer sealed the pulp chamber floors, whereas in the FR-NFRC (foundation restoration, no fiber-reinforced composite) and FR-FRC (foundation restoration, fiber-reinforced composite) groups, a 3.0-mm foundation restoration was used. NFR-NFRC and FR-NFRC groups were restored with composite resin overlays, whereas NFR-FRC and FR-FRC groups were restored with fiber-reinforced composite resin overlays. All specimens were subjected to mechanical loading in a computer-controlled masticator and then the fracture resistance was evaluated. Differences in means were compared using two-way ANOVA and Tukey's test. The level of significance was set at ɑ = 0.05. RESULTS All specimens successfully completed the fatigue test. The least fracture-resistant group was NFR-FRC, exceeded by FR-NFRC, NFR-NFRC, and FR-FRC, in that order, with FR-FRC being the most fracture-resistant group. Statistically significant differences were detected between the pairs NFR-NFRC/FR-FRC (p = 0.001), NFR-FRC/FR-FRC (p = 0.001), and FR-NFRC/FR-FRC (p = 0.001). Eight vertical root fractures occurred in group FR-NFRC, six in group NFR-NFRC, four in group NFR-FRC, and none occurred in group FR-FRC. CONCLUSIONS Within the limitations of this in vitro study, the incorporation of glass fibers and the presence of a foundation restoration were found to increase the fracture resistance and can favorably influence the fracture mode.","PeriodicalId":94234,"journal":{"name":"The journal of adhesive dentistry","volume":"81 1","pages":"143-9"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83897274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Esteves-Oliveira, P. Jansen, M. Wehner, A. Dohrn, M. BeLLO-siLva, C. P. Eduardo, H. Meyer-Lueckel
PURPOSE To evaluate the suitability of an ultra-short pulsed laser (USPL) to treat zirconia ceramic surfaces and increase their adhesion to dual-curing resin cement. MATERIALS AND METHODS Twenty 10 × 10 × 5 mm³ blocks were prepared from a zirconia ceramic (Y-TZP). The specimens were polished and randomly assigned to four groups (n = 5) which received the following surface treatments: sandblasting (SB) with Al₂O₃ particles and silica coating (SC) with SiO₂ particles as positive controls; two groups received USPL irradiation, one with 10 scan repetitions (L10) and the other with 20 (L20). Laser irradiation was performed at 1030 nm, 2.3 J/cm², 6 ps pulse duration. The ceramic blocks were duplicated in composite resin and cemented with a dual-curing resin cement. Half of the blocks were then stored in water (37°C) for 24 h and the other half for 1 month. At each time, 40 to 60 sticks per group were subjected to microtensile bond strength testing. Data were analyzed statistically using the Kruskal-Wallis test (α = 0.05). RESULTS Laser-treated zirconia presented statistically significantly higher roughness than did SB and SC. After 24 h, the highest bond strength means (MPa) were achieved by L10 (42.3 ± 10.8) and L20 (37.9 ± 14.4), and both of them were statistically significantly higher than SB (22.0 ± 5.3) and SC (20.8 ± 7.1) (p < 0.05). After 1 month of storage, L10- and L20-treated zirconia still showed significantly higher bond strengths than did SB- and SC-treated zirconia (p < 0.05). CONCLUSION USPL irradiation significantly increases bond strength of zirconia ceramic to dual-curing resin cement and might be an alternative for improving adhesion to this material.
{"title":"Surface Characterization and Short-term Adhesion to Zirconia after Ultra-short Pulsed Laser Irradiation.","authors":"M. Esteves-Oliveira, P. Jansen, M. Wehner, A. Dohrn, M. BeLLO-siLva, C. P. Eduardo, H. Meyer-Lueckel","doi":"10.3290/j.jad.a36918","DOIUrl":"https://doi.org/10.3290/j.jad.a36918","url":null,"abstract":"PURPOSE To evaluate the suitability of an ultra-short pulsed laser (USPL) to treat zirconia ceramic surfaces and increase their adhesion to dual-curing resin cement. MATERIALS AND METHODS Twenty 10 × 10 × 5 mm³ blocks were prepared from a zirconia ceramic (Y-TZP). The specimens were polished and randomly assigned to four groups (n = 5) which received the following surface treatments: sandblasting (SB) with Al₂O₃ particles and silica coating (SC) with SiO₂ particles as positive controls; two groups received USPL irradiation, one with 10 scan repetitions (L10) and the other with 20 (L20). Laser irradiation was performed at 1030 nm, 2.3 J/cm², 6 ps pulse duration. The ceramic blocks were duplicated in composite resin and cemented with a dual-curing resin cement. Half of the blocks were then stored in water (37°C) for 24 h and the other half for 1 month. At each time, 40 to 60 sticks per group were subjected to microtensile bond strength testing. Data were analyzed statistically using the Kruskal-Wallis test (α = 0.05). RESULTS Laser-treated zirconia presented statistically significantly higher roughness than did SB and SC. After 24 h, the highest bond strength means (MPa) were achieved by L10 (42.3 ± 10.8) and L20 (37.9 ± 14.4), and both of them were statistically significantly higher than SB (22.0 ± 5.3) and SC (20.8 ± 7.1) (p < 0.05). After 1 month of storage, L10- and L20-treated zirconia still showed significantly higher bond strengths than did SB- and SC-treated zirconia (p < 0.05). CONCLUSION USPL irradiation significantly increases bond strength of zirconia ceramic to dual-curing resin cement and might be an alternative for improving adhesion to this material.","PeriodicalId":94234,"journal":{"name":"The journal of adhesive dentistry","volume":"24 1","pages":"483-492"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72689859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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