International journal of medicinal mushrooms最新文献

Sanghuangporus is a precious genus of medicinal mushroom and internationally recognized as one of the best medicinal species for anti-cancer effects. In this study, the morphology, functional components and activities of four species Sanghuangporus were evaluated and analyzed. The thickness, color, ring lines and density of Sanghuangporus fruiting bodies parasitic on different species of trees had certain differences. The research result on nutritional components indicated that four species Sanghuangporus contained the same species of amino acids, but the contents were different. However different Sanghuangporus contained diverse species and contents of fatty acids and microelements. There was a characteristic nickel element in S. sanghuang. At the same time, only S. lonicericola and S. sanghuang contained antimony. In the results of functional components, the infrared spectra of different Sanghuangporus demonstrated high similarity, but there were differences in the species and content of functional groups, and the chemical composition and proportion of the four species also were extremely different. UPLC-QTOF-MS/MS identified 72 characteristic compounds in the Sanghuangporus. S. lonicericola uniquely contained hydroxycitric acid, S. toxicodendri contained fisetin. S. sanghuang contained 6-hydroxypurine, D-raffinose and 3-hydroxyanthranilic acid. The results of antioxidant and hypoglycemic experiments exhibited that the four species of Sanghuangporus had different activity capacity. S. vaninii showed the highest antioxidant activity and could be used in the development of natural antioxidants. S. sanghuang had good hypoglycemic activity, which was more suitable for the development of hypoglycemic products. This survey provides the basis and theory for the study of the components and activities of different species of Sanghuangporus and contributes new resources to dietary supplement and medicine.

Tricholomopsis aurea is used as food in different parts of the world, but has not been investigated for its nutrients, metabolites, and biological potentials like other edible mushrooms. This work aimed to quantify the metabolic and nutrient content of T. aurea and evaluate the antioxidant and anti-inflammatory activities of the extract and isolated compounds. The method employed involves chromatographic, spectroscopic, bovine serum albumin microplate, analytical and standard assays. Oleic, elaidic, petroselinic acids, ergosterol, ergosterol peroxide, 22E724R)-24-ethylcholesta-5,7,22-trien-3β-ol, and adenosine were isolated and identified using 1D and 2D-NMR spectroscopy and spectrometric data. The metabolic content revealed high phenolics (799.62 μgGaE/g of dry matter (DM)), low flavonoids (24.54 μgQE/g DM), alkaloids (32.92 μgQiE/g DM), and saponins (88.00 μgQSE/g DM). The nutrients content was made up of proteins (4.79%), lipids (10.43%), fibers (16.01%), ashes (15.96%), carbohydrates (8.74%), dry matter (85.93%), and moisture (14.07%) with energy value of 362.89 kcal. In mg/100 g, the minerals were phosphorus (283.97%), calcium (817.25%), potassium (67.10%), magnesium (94.42%), iron (57.27%), and sodium (74.4%). The extract displayed the antioxidant activity against TAC and FRAP (100-1000 μg/mL), DPPH (SC50 of 248.95 μg/mL) and ABTS (SC50 of 180.7 μg/mL), while the test compounds were not active. The extract, adenosine, ergosterol peroxide, and ergosterol showed anti-inflammatory activity with IC50 of 49.19 μg/mL, 4.91 μg/mL, 6.85 μg/mL, and 29.51 μg/mL, respectively. Conclusively, this study will help to promote the application of T. aurea in traditional dishes and functional or nutraceutical foods.

Mushrooms are widely recognized for their rich nutritional profile and diverse array of bioactive compounds, including proteins, polysaccharides, and secondary metabolites, which contribute to various health benefits. Among these compounds, phospholipids stand out due to their essential role in cellular processes and their potential applications in food, nutraceuticals, and pharmaceuticals. This review comprehensively examines the phospholipid composition in mushrooms, highlighting the methods of analysis, and delving into their functional applications. By comparing lipid profiles across different stages of growth and between various species, this review highlights the significant role of phospholipids in mushroom cellular structure and function. The review also discusses the biosynthetic pathways and extraction methods employed for this class of compounds. By shedding light on this underexplored aspect of mushroom chemistry, this work aims to spur further research and innovation in the utilization of mushrooms in food science and medicine.

Fruiting bodies of mushrooms represent an important functional food owing to various beneficial health enhancing effects. The edible mushroom Pholiota limonella (Strophariaceae, Agaricomycetes) has been found to contain various bioactive components. In this study, the proximate amino and fatty acids, as well as volatile compounds of P. limonella were analyzed to assess its nutritional and aromatic profiles. The in vitro antioxidant activity of P. limonella extract was evaluated using DPPH, ABTS, and hydroxyl radical-scavenging assays. The results showed that P. limonella possesses antioxidant activity and provided a good research basis for the comprehensive development of P. limonella as functional food product.

Spent mushroom cultivation substrate is sometimes used to make organic fertilizers, but most of it is discarded. This study aimed to evaluate the efficiency of spent mushroom substrate (SMS) as a substitute substrate for cultivating Pleurotus pulmonarius. Seven types of SMS were employed to cultivate P. pulmonarius to assess yield, biological efficiency, nutritional composition, and bioactive compound content in the fruiting bodies. The SMS of Agaricus marmoreus showed the highest yield (253.88 g/bag) and biological efficiency (63.47%). Variations in the nutritional composition of fruiting bodies cultivated on different SMSs were observed. The highest levels of ergothioneine (2.17 mg/g dry matter) and adenosine (7.71 mg/g dry matter) were found in fruiting bodies grown on P. pulmonarius and Hymenopellis radicata SMSs, respectively. These findings present a promising strategy to reduce cultivation costs associated with P. pulmonarius and manage SMSs effectively.

Cordyceps militaris, an entomopathogenic fungus traditionally used in East Asian medicine, contains 1,3-β-glucans with well-known immunomodulatory properties. Our previous research has demonstrated that both fruit body powder and hot water extract of C. militaris can activate bone marrow-derived dendritic cells through dectin-1 signaling. However, the immunological effects on Fms-like tyrosine kinase 3 ligand-induced dendritic cells (fDCs), which closely resemble steady-state conventional dendritic cells in vivo, remain unexplored. In this study, we investigated the expression of dectin-1 in fDCs and its response to C. militaris fruit body powder (RK). Flow cytometric analysis revealed that conventional and plasmacytoid dendritic cells within the fDC population expressed dectin-1, with conventional dendritic cells showing particularly robust expression. Similar expression patterns were observed in freshly isolated splenic DCs. Importantly, RK induced significant tumor necrosis factor-α production in wild-type fDCs, whereas this effect was completely abolished in dectin-1-knockout fDCs. These findings demonstrate that C. militaris fruit-body powder activates fDCs through a dectin-1-dependent pathway, providing new insights into its immunomodulatory mechanisms and potential therapeutic applications.

An innovative menu based on the use of fresh and dried medicinal mushrooms, which is energetic, healthy, easy to make, and adaptable to any type of diet, is presented. Three edible mushrooms (Lentinula edodes, Grifola frondosa, and Pleurotus ostreatus), which are known for their nutritional and medicinal properties, are paired with typical Sicilian products and used for the preparation of a menu consisting of an appetizer, a first and second course, pre-dessert, and dessert. The proposed menu meets all our criteria.

The tiger milk mushroom (Lignosus rhinocerus), a Southeast Asian medicinal mushroom, has traditional uses for respiratory ailments, wound healing, and general health. This study evaluated the acute dermal toxicity of L. rhinocerus sclerotium (TM02Ŵ) and cold-water extract (xLrŴ) for safe topical application. Healthy female Sprague Dawley rats were topically administered TM02Ŵ or xLrŴ at 2000 mg/kg body weight, while physiological saline served as a control. Visual toxicity observations, body and organ weight measurements, food and water intake, hematological and serum biochemical analyses, and histological examinations were performed. Findings were contextualized with prior oral toxicity studies of L. rhinocerus. Both TM02Ŵ and xLrŴ demonstrated excellent dermal safety with no observed mortality, behavioural abnormalities, or significant differences in food or water intake, body weight, or organ weights compared with controls. Hematological and biochemical parameters remained within normal ranges, and histopathology of the skin and major organs revealed no adverse effects. Consistent with earlier chronic oral toxicity studies, the preparations showed no evidence of systemic toxicity or organ damage. TM02Ŵ and xLrŴ are classified as Category 5/Unclassified under the Globally Harmonized System (GHS) criteria, affirming their suitability for topical use. These findings, coupled with earlier oral toxicity studies, highlight the safety of L. rhinocerus as a versatile ingredient for therapeutic and cosmetic applications. Further research on chronic dermal exposure is recommended to reinforce its use in skincare.

The global increase in cancer cases, particularly prostate cancer, poses a significant health challenge worldwide. Conventional treatments such as surgery, radiation therapy, hormone therapy, chemotherapy, and immunotherapy offer valuable options but are associated with limitations and potential side effects. As a result, there is growing interest in complementary therapies, including natural compounds such as β-glucans, derived from sources such as yeast and mushrooms. In this review, we explored the potential therapeutic role of medicinal mushrooms β-glucan in prostate cancer treatment. β-glucans has demonstrated anti-cancer properties in preclinical studies, including inhibition of proliferation, induction of apoptosis, and modulation of immune responses. Studies in prostate cancer cell lines and animal models have shown promising results, with β-glucan inhibiting tumor growth, inducing DNA damage, and regulating tumor markers such as p53 and prostate specific antigen. β-glucans acts through various pathways, including stimulation of dendritic cells, modulation of cytokine secretion, suppression of myeloid-derived suppressor cells, and enhancement of immune responses. Moreover, β-glucans exhibits anti-androgenic and immune-modulatory effects, making it a promising candidate for prostate cancer treatment. In this study, we also focused on the mechanism of action of β-glucans through various pathways including tumor cell death by oxidative stress created through ROS generation and autophagy. Although preclinical studies support the potential therapeutic efficacy of medicinal mushrooms β-glucans, further research is needed to elucidate its clinical utility and safety in human trials.

Physiological studies were performed to investigate the optimal mycelial growth parameters for Ganoderma resinaceum EGM (AC: LN774970) and G. mbrekobenum EGDA (previously identified as Ganoderma sp. EGDA, AC: LN774971). The study found that the highest growth of mycelia was at 11 d for Ganoderma species. The nutritional studies were carried out to know the best growth conditions including temperature, culture media, pH, carbon source, and nitrogen source. The most appropriate temperature for the highest growth for Ganoderma species was at 30°C; this benefit enables the cultivation of these species in the spring. The most favorable media for their growth was potato dextrose broth as semi-synthetic followed by Saburaoud broth as synthetic media. They also could grow at pH ranges from 3.0-9.0, with the highest mycelial growth at pH 5.0. This study confirmed that both Ganoderma species prefer glucose as a carbon source and peptone as an organic nitrogen source. Decolorization of poly R associated with peptone was 21.37% and 18.66% for both EGM and EGDA strains, respectively. The highest cellulase production was achieved after 11 d of incubation with activity reached 0.305 μmole/mL/min and 0.236 μmole/mL/min for G. mbrekobenum EGDA and G. resinaceum EGM, respectively. The highest laccase activity was obtained after 8, 12 d of incubation with a value of 5.53 μmole/mL/min and 31.32 μmole/mL/min, for EGM and EGDA strains, respectively. Moreover, the highest peroxidase activity for both EGM and EGDA strains was recorded after 26 and 20 d of incubation with a value of 0.333 μmole/mL/min and 0.230 μmole/mL/min, respectively. G. mbrekobenum EGDA possessed higher flavonoids and phenolic compounds (4.098 mg/g and 6.298 mg/g dry weight, respectively) than G. resinaceum EGM (1.137 mg/g and 2.420 mg/g dry weight, respectively).